LOCUS       AB812074               17248 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: E29518.
ACCESSION   AB812074
VERSION     AB812074.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 17248)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..17248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E29518"
                     /db_xref="taxon:562"
                     /note="serogroup:O176"
     gene            <1..961
                     /gene="gne"
     CDS             <1..961
                     /gene="gnu"
                     /note="O176_01"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAQ01929.1"
                     /translation="FVGTRLLETAIADFNIKNLDKQQSHFYPEITQIGDVRDQQALDQ
                     ALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVKNIIFTSSVAVYG
                     LNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIRPTVIFGERNRGN
                     VYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGYEVYNYVDKPDLN
                     MNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAVSSVRVKKFCATT
                     QFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            1203..2096
                     /gene="galF"
     CDS             1203..2096
                     /gene="galF"
                     /note="O176_02"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01930.1"
                     /translation="MTTLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWTELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     CDS             2539..3123
                     /note="O176_03; transposse of ISAs1 family"
                     /gene="ISAs1"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     CDS             3320..3673
                     /note="O176_04; transposse of ISAs1 family"
                     /gene="ISAs2"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     CDS             4008..5183
                     /note="O176_05"
                     /gene="wfbV"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01931.1"
                     /translation="MKILYVSARFPWPLLTGDALRAYNQIKSLSKFHSIDVFSIEAPP
                     VSRGDIDLYISESMSSQISKLQKIINIISYGKDTAFQCAMYNDMKSWQELRRLIISNK
                     YDVIIFQLVRLEYLIKKTVELKNQLGLDISIYCDYVDALSLNMENRTATEGLLLKKIC
                     SSESRKLKKIENEIYDLVDVKIIISERDGKYINNKGFNVVPNGVTIPVRNESLYHNES
                     DGVVNLSFWGNMSYYPNVRAAQYLADIFRQLEVGKYKLHIIGAKPSKKVRDLGNSDNI
                     IVHGFIDDLPNFLANMDLAVFPIFDGSGLQNKVLEAFALEIPVITTNVVLDSIPKLKK
                     FAMVANCKADFIRNIESFELSKEKSPSESGVLKVLNEHYNWENINQVMIQNEKNTAF"
     CDS             5161..6180
                     /note="O176_06"
                     /gene="wfbW"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01932.1"
                     /translation="MKKILLFNFYNGVLYRGIPIYSSNLKIGLEKEGCEVNEWKCPNA
                     LNKMPRFLLDLLFVFSEQILMPLICFFGRYDKVIYPYNSCSILSAIVGKSLLIIHDFI
                     PNKKNKSKLSLSALYIVLTQRVHTLLKRDVAFVSRTTQRIGKNVSWLKKCGVYLFPNS
                     FYILERELANLKCKFFQKDEYAVLISGNGTNKEFTKAMELWKGCNICYSHHLKVIGLG
                     GHQEWAQKILKSLNINNVSILPVLSDSALLCEIINASFVWAHSNHEGFGRPVIEGRMC
                     GKNVLATNISAFREHKDDNVFLYNQNSFCENLRLAHSFGCKPNYKIKYHLILEEQIKK
                     WLMKN"
     gene            6162..7598
                     /gene="manC"
     CDS             6162..7598
                     /gene="manC"
                     /note="O176_07"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="BAQ01933.1"
                     /translation="MVDEKLIPIIMAGGTGSRLWPLSRELYPKQFLSLQHELTMLQST
                     IMRLDGITNCEPVVICNESHRFIVAEQLLQIQKLAKNIILEPVGRNTAPAVALAALTA
                     KAIHSDEDPLLLILAADHVFTDEIAFRNSISKAIPFANVGKLVTFGILPNKPETGYGY
                     ILRGKKIKLESDDYAFEVERFVEKPDIETAEEYVSNGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILLACEKSISEMSFDLDFIRINEEAFASCPDESIDYAVMEHTSDAVVIPMDAG
                     WSDVGSWSSLWEVSIQNSEGNVCHGDVINYKTKNSYVYSESSLVTTIGVKDLVIVQTK
                     DALLVAHRDAVQDVKKVVEKLKSDGRPEYQEHREVYRPWGKYDSIDSGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTINGDTRLIGENESIFIPLGARHCLENPGKI
                     PLDLIEVRSGPYLEEDDIVRLSDRYGRK"
     gene            7649..9019
                     /gene="manB"
     CDS             7649..9019
                     /gene="manB"
                     /note="O176_08"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="BAQ01934.1"
                     /translation="MNILSCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINCTVHNQKSIINK
                     ILNEFQAQAKNIDYTDGISMEFERWRFNLRSSNTEPVLRLNVESKKDKDLMEEKTKEL
                     LSRIVK"
     gene            9142..10473
                     /gene="wzy"
     CDS             9142..10473
                     /gene="wzy"
                     /note="O176_09"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01935.1"
                     /translation="MILYKERSVQANVITKLMAICIVICFAMFILLLKNMLGNNGYAT
                     LLHVLAPFLSICLIAVMIFFYIKRASFISLQLMYAFAFSLIIGVPGIYLLNFRNPVKG
                     FEIVCLWAILLNIVLYFCSYNNKTITQRKKVNKYFVVIFVIVGICQLIKTGLYFIFIV
                     NSGVGHLAIYTEGDALVSQVPFVIRAISGMSLIMSLATFYYRASTGVRWFSFLLLASE
                     LLIGIRSKFFFSLISLIILSLYCNKELIKKRFLKISRLLYLVIGFVLFSLVSYYREGY
                     DINFVKYMVIVLDSLSSTLAGLQDIFVLPDSQGWNRLDPHLIITQLFPISGLGFLNST
                     QIYKEFSVIVLGDISSGIALSSSGILESTILDVDFNFFIYLSYLLLMLSLIQHFLNSP
                     KVFLNFIAIAMLPGFLYSIRGELILPIAYIIKSSPIIIVSNFLVSDKNADE"
     gene            10470..11708
                     /gene="wzx"
     CDS             10470..11708
                     /gene="wzx"
                     /note="O176_10"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01936.1"
                     /translation="MNMIRDIFNDGIYSLINKIGYFGAKTIAIILITRTLGVDEGSQF
                     IFLISIIEIMRVIGDFGVDVYVIRKYGDVPNKERLLCTVFYQKIIVGFVFAAILIVYC
                     SILKYNASIYVPLSLSIIFSLLFNLSSSYFQSLNINKAITTSIISASIFIGVLFSSFY
                     YFNVVVNAYHFLVIEIVYCMSVSFTLINKTDLKIIRQFKKVDISAILYLYRNTYSIGI
                     TALIVIIYSRLDNIYLKLYDEQSLAVYGQIFRFVDPLVMISSVFSTVAYAKFSNFKLH
                     NNEASIKSFLLMIVIYILVSFLIYYYVLISIGQWFILPTSLFKPLLIGFLCMASIKCM
                     NGSLTAILQSQGLYKIGLYISILCIIIAVPLMYVFVKHYHALGCIYAVLIVETVSFIF
                     LSASVYFLTSNRNHKDLNVK"
     gene            11976..13382
                     /gene="gnd"
     CDS             11976..13382
                     /gene="gnd"
                     /note="O176_11"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01937.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAEDPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            13632..14798
                     /gene="ugd"
     CDS             13632..14798
                     /gene="ugd"
                     /note="O176_12"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01938.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            14908..15921
                     /gene="wzz"
     CDS             14908..15921
                     /gene="wzz"
                     /note="O176_13"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01939.1"
                     /translation="MRTWKFPSVRVMMRVENNNVSGQNHDPEQIDLIDLLMQLWRGKM
                     TIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAMNVIYGQAAPKVSD
                     LQETLIGRFSSAFSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKL
                     AQYIQQVDDKVNQELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQY
                     ANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDI
                     ESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNY
                     NAK"
     gene            complement(16104..16715)
                     /gene="hisI"
     CDS             complement(16104..16715)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01940.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIASDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLTTVIENLRKRHQ"
BASE COUNT     5043 a   3247 c   3797 g   5161 t
ORIGIN
        1 cttcgtagga acccgactac ttgaaacggc aattgctgac tttaatatca agaacctgga
       61 caaacagcag agccactttt atccagaaat cacacagatt ggcgatgttc gtgatcaaca
      121 ggcactcgac caggcgttag ccggttttga cactgttgtg ctactggcag cggaacaccg
      181 cgatgacgtc agccctactt ctctctatta tgatgtcaac gttcagggta cccgcaatgt
      241 gctggcggcc atggaaaaaa atggcgttaa aaatatcatc tttaccagtt ccgttgctgt
      301 ttatggtttg aacaaacaca accctgacga aaaccatcca cacgatcctt tcaaccacta
      361 cggcaaaagt aagtggcagg cagaggaagt gctgcgtgaa tggtataaca aagcaccaac
      421 agaacgttca ttaactatca tccgtcctac cgttatcttc ggtgaacgca accgcggtaa
      481 cgtctataac ttgctgaaac agatcgctgg cggcaagttt atgatggtgg gcgcagggac
      541 taactataag tccatggctt atgttggaaa cattgttgag tttatcaagt acaaactgaa
      601 gaatgttgcc gcaggttatg aggtttataa ctacgttgat aagccagacc tgaacatgaa
      661 ccagttggtt gctgaagttg aacaaagcct gaacaaaaag atcccttcta tgcacttgcc
      721 ttacccacta ggaatgctgg gtggatattg ttttgatatc ctgagcaaaa ttacgggcaa
      781 aaaatacgct gtcagctcag tgcgcgtgaa aaaattctgc gcaacaacac agtttgacgc
      841 aacgaaagtg cattcttcag gttttgtggc accgtatacg ctgtcgcaag gtctggatcg
      901 aactcttcag tatgaattcg tccatgccaa aaaagacgac ataacgtttg tttctgagta
      961 atgcagacga gtcagagaaa aaaggaccag ggtggactac gattattcgg aaagaggtac
     1021 tgattaaatt atcagtatgt tgatttccga gtaatccgag taacccagat gctgatggtg
     1081 tgtagtgtta tcgtaaggtt taatcaaagt tatgcggaac taagctttgg tatatagcca
     1141 taccgtttgg ctaaccgatg gcagtaattt acgcactatc agtcacttag aggtaggaaa
     1201 gaatgacgac tttaaaagca gttataccgg tagcaggtct tgggatgcat atgttgcctg
     1261 ccactaaggc gattcccaaa gagatgctac cgatcgtcga caagccaatg attcagtaca
     1321 ttgttgacga gattgtggct gcagggatca aagaaatcct cctggtaact catgcgtcca
     1381 agaacgcggt cgaaaaccac ttcgacacct cttatgaatt agaatctctc cttgaacagc
     1441 gcgtgaagcg tcaactactg gcggaagtgc aatctatctg cccgccgggc gtgactatta
     1501 tgaacgtgcg tcagggcgaa cctttaggtt taggtcactc cattttgtgt gcacgacccg
     1561 ccattggtga caatccattt gtcgtggtgc tgccagacgt tgtgatcgac gacgccagtg
     1621 ccgatccgct gcgttacaac cttgctgcca tgattgcacg cttcaacgaa acgggccgca
     1681 gccaggtgct ggcaaaacgt atgccgggtg acctctctga atactccgtc attcagacca
     1741 aagagccgct ggaccgtgaa ggtaaagtca gccgcattgt tgaatttatc gaaaaaccgg
     1801 atcagccgca gacgctggac tcagacatca tggccgttgg tcgctatgtg ctttctgccg
     1861 atatttggac ggaacttgaa cgcactcagc ctggtgcatg gggacgtatt cagctgactg
     1921 atgccattgc tgaactggcg aaaaaacagt ccgttgatgc aatgctgatg actggagaca
     1981 gctacgactg cggtaaaaaa atgggttata tgcaggcgtt tgtgaagtat ggactacgca
     2041 acctgaaaga aggggcgaag ttccgtaaag gtattgagaa gctgttaagc gaataatgaa
     2101 aatctgaccg gatgtaacgg ttgataagaa aattataacg gcagtgaaga ttcgtggtga
     2161 aagtaatttg ttgcgaatat tcctgccgtt attttatata aacaatcaga ataacaatga
     2221 gttagcaata ggattttagt caaagttttc caggattttc cttgtttcca gagcggattg
     2281 gtaagacaat tagcgtttga atttttcggg tttagcgcga gtgggtaacg ctcgtcacat
     2341 cgtagacatg catgcagtgc tctggtagct gtaaagccag gggcggtagc gtgccaagga
     2401 aagatcacgt aacgctgctt ttttggacta aataattcgc attttatgtt taaaattgag
     2461 ctattcctca ttacctgaag ctgtttattt attgcttata catgatcaaa tactccttac
     2521 ataattaagg agaacaaaat ggaacttaaa aaattgatgg aacatttttc tattattccc
     2581 gattacagac aagcctggaa agtggaacat aaattgtcag atattctact gttgactatt
     2641 tgtgccgtca tttctggtgc agaaggctgg gaagatagag aggattttgg agaaacacat
     2701 ctcgattttt tgaagcaata tggtgatttt gaaaatggta ttcctgttca cgataccatt
     2761 gccagagttg tatcctgtat cagtcctgca aaatttcacg agtgctttat taactggatg
     2821 cgtgactgcc attcctcaaa tgataaagac gttattgcaa ttgatggaaa aacgctccgg
     2881 cactcttatg acaagagtcg ccgcaaggga gcgattcatg tcattagtgc gttctcaaca
     2941 atgcacagtg tggttctcgg cagattaaga cggatgagaa atccaatgag attacagcca
     3001 ttcctgaact tcttaacgtg ctggatatta aaggaaaaat catcacaact gatgcgatgg
     3061 gttgccagaa agatattgca gagaagatac aaaaacaggg aggtgattat ttattcgcgg
     3121 taaaaggaaa ccaggggcgg ctaaataaag cctttgagga aaaatttccg ctgaaagaat
     3181 taaataatcc agagcatgac agttacgcaa tcagtgaaaa gagtcacggc agagaagaaa
     3241 tccgtcttca tattgtttgc gatgtccctg atgaacttat tgatttcacg ttgaatggaa
     3301 atggctgaag aaattatgcg tggcagtctc ctttcggtcc ataatagcag aacaaaagaa
     3361 agagccagaa atgacggtca gatattatat cagttctgat gatttaaccc cagagaagtt
     3421 cgccagagca atccgaaacc actggcacgt ggagaataag ctgcactggc gtctggatgt
     3481 ggtaatgaat gaagacggct gcaaaataag aagaggaaac gcagcagaat tattttcagg
     3541 gatacggcac atcgctatca atattttgac gaatgataag gtattcaagg cagggttaag
     3601 acgtaagatg cgaaaagcag ccatggacag aaactatctg gcgtcagtcc ttgcgtggag
     3661 cgggctttcg taatcttgcc ctgtctccag ctcacgttca gaggaagtct gctgattttg
     3721 ctgtttactg cgccagttgt agagctgcga ttcatacagg ctgagttcgc gggctgcggc
     3781 agcaataccg atgcgttccg caagcttcag atcttcactg cgaaattcag gcgaatgctg
     3841 tttatggttt tttttactgg ttgatactgt ttttgccatg tgagagtctc ctctgactga
     3901 gagtatactc acttagctgc gtgtccacta ttgctgggta agatcaatac tgaaatatta
     3961 atttgaagct ttaacgatca tttttatgta gaatttcaag gttagttatg aaaatattat
     4021 atgtaagtgc acgctttcca tggccattac tgactggcga tgctctccga gcatacaacc
     4081 aaattaaatc actttcaaag ttccactcga tagatgtatt ttctattgaa gctcctcctg
     4141 tatctagagg ggatatagac ttatacataa gtgagagtat gtctagtcaa atttcaaaat
     4201 tacaaaaaat aataaacatc atatcttacg gtaaagatac agcatttcaa tgtgcaatgt
     4261 ataacgacat gaaatcgtgg caagaactta gaaggttaat aatatcaaat aaatatgatg
     4321 tgattatatt tcaattggtt aggttggaat atttaataaa gaaaactgta gaattaaaga
     4381 atcagcttgg tttagatatt tcgatatatt gtgattatgt tgatgcactt tcattaaata
     4441 tggaaaatcg tacagcgacc gaaggtttac tattgaaaaa aatatgttca agtgaaagcc
     4501 ggaagcttaa aaaaattgag aatgaaatat atgacttagt tgatgttaaa ataattatat
     4561 cagaaagaga tggcaaatat ataaataata aaggatttaa tgttgttcct aatggagtga
     4621 caatcccagt gcgcaatgaa agcttatatc ataacgaaag tgatggggtt gtgaatttgt
     4681 cattttgggg taatatgagt tattatccca atgttagagc ggctcaatat ttagctgata
     4741 tatttaggca attagaagtt ggtaaatata aactccatat tattggtgct aaaccaagca
     4801 aaaaggtacg tgatttagga aatagtgata atataattgt tcatggtttt atagatgact
     4861 tacctaattt tttggcgaat atggatttgg cggtatttcc tatttttgat ggttctgggt
     4921 tgcaaaataa agtactagaa gcgtttgctt tagaaattcc tgtaatcaca acaaatgttg
     4981 tattagactc aataccaaaa ttaaaaaaat ttgctatggt tgcaaactgt aaagcggatt
     5041 ttatacggaa tatcgaatcc tttgaattat cgaaggaaaa atcaccttca gagtctggtg
     5101 tattaaaagt tttaaatgag cattataatt gggaaaatat caatcaggta atgatacaaa
     5161 atgaaaaaaa tactgctttt taatttttat aacggggtgt tatatagagg tatacctatt
     5221 tattcatcta acttaaagat aggtttagag aaagaaggat gtgaagttaa tgaatggaaa
     5281 tgtccaaatg cattaaataa aatgccaagg tttttattgg atctgttgtt tgtttttagt
     5341 gaacaaattt taatgccttt aatttgtttt tttggacgtt atgataaggt tatatatcct
     5401 tataattcat gttctatttt atccgctata gttggaaaaa gtttgctgat tatacatgat
     5461 tttatcccaa ataaaaaaaa caaatcaaaa ttaagcttat ctgcattata tattgttctg
     5521 actcagcgag tgcatactct tttaaaaaga gatgtggctt ttgtatctag aacaacgcaa
     5581 agaataggga aaaacgtatc ttggttaaaa aaatgtggag tatatctatt ccccaattca
     5641 ttctatattt tagagcgtga attggcaaat ttgaaatgta agttctttca aaaggatgaa
     5701 tatgctgttc ttatatctgg caacggaaca aataaagaat ttacaaaagc aatggaattg
     5761 tggaaaggat gtaatatttg ttattctcat catctaaaag ttatagggtt agggggacat
     5821 caggaatggg cgcaaaaaat attaaaatcg cttaatatta ataatgtttc tattttacct
     5881 gttctttcag acagtgcgtt gctttgcgaa attattaatg cttcatttgt gtgggcgcat
     5941 tcaaatcacg aaggatttgg tcgccctgtt attgaaggtc gtatgtgtgg gaaaaatgta
     6001 ttagcaacta atatatccgc attcagagag cataaagatg ataatgtctt tttatataat
     6061 caaaactctt tttgcgaaaa cttaagatta gcccattcat ttggttgtaa accgaactat
     6121 aagattaagt atcatttaat tttagaggaa cagataaaaa aatggttgat gaaaaattaa
     6181 tccctataat aatggctggt ggtactggca gtagattgtg gccattgtca agagaactct
     6241 acccaaaaca atttctttcc ttgcaacatg aattaacaat gctgcaatca accattatgc
     6301 gactagatgg tattacaaac tgtgagccag ttgttatttg taatgaaagt catcggttta
     6361 tcgtcgctga gcagttgtta caaattcaga agttagcaaa aaatataata ttagagcctg
     6421 taggacgtaa cacagcccca gctgttgcgt tagctgcgtt aacagcaaaa gcaattcact
     6481 cagatgaaga tccattatta ctaatattag ctgcagacca tgtttttact gacgaaatag
     6541 catttagaaa ctctattagt aaagcgatac cgtttgctaa tgtcgggaag cttgtaactt
     6601 ttggtattct gccaaataag cctgaaacag ggtatggata tattctacgt ggaaaaaaaa
     6661 tcaaacttga aagtgatgat tatgctttcg aagtagagcg ttttgttgaa aaaccagata
     6721 ttgaaacagc tgaagaatat gtttcaaatg gcgaatatta ctggaatagt gggatgtttc
     6781 tttttcgcgc aggacgttat cttgaagagc ttaaaaaata tcgtccagat atactattag
     6841 cttgcgaaaa atcgatatca gagatgagtt ttgatcttga ttttattcga attaacgagg
     6901 aagcgtttgc ctcctgtcca gatgagtcta ttgattatgc tgttatggag catacatctg
     6961 atgctgtcgt tatacctatg gatgcagggt ggagcgatgt tggctcttgg tcatctttat
     7021 gggaggtaag tattcaaaac tcggaaggga acgtttgcca cggtgatgtg atcaattaca
     7081 aaactaaaaa tagttatgtg tattctgagt ctagcttagt caccacaatc ggggtgaaag
     7141 atttagtaat tgttcaaact aaagatgcgt tactagtagc tcaccgtgat gctgtacagg
     7201 atgttaaaaa agttgtcgaa aaactcaaat cagatggtcg tcctgagtat caagaacatc
     7261 gtgaggtata tcgtccgtgg ggtaagtatg attcaatcga tagcggtgat cgttatcaag
     7321 taaaacgtat tacagtaaaa cccggcgaag gtctatcagt gcaaatgcac catcaccgtg
     7381 cagagcactg ggttgtggtg gctggtacgg ctaaagtaac catcaatggt gatactagat
     7441 taattggtga aaatgagtct atatttattc cattaggagc acgtcattgt cttgaaaacc
     7501 cggggaaaat tcctcttgat ctgattgagg tgcgttccgg gccttatctt gaggaagatg
     7561 atattgtgcg tttatcggat cgttatgggc gcaaatgaaa ttactcattt atttctttaa
     7621 ataaatcaaa cgttaagtaa attgaaaaat gaatatatta tcttgcttta aagcctatga
     7681 tattcgcggg aaattaggcg aagaactaaa tgaagatatc gcctggcgca ttggtcgcgc
     7741 ctatggcgaa tttctcaaac cgaaaaccat tgtgttaggc ggtgatgtcc gcctgaccag
     7801 cgaaacctta aaactggcgc tggcgaaagg tttacaggat gcgggcgtcg atgtgctgga
     7861 tattggcatg tccggcaccg aagagatcta tttcgccacg ttccatctcg gcgtggatgg
     7921 cggcattgaa gttaccgcca gccataaccc gatggattat aacggcatga agctggtgcg
     7981 cgaaggggct cgcccgatca gcggtgatac cggactacga gatgtccagc gtctggcaga
     8041 agccaacgac tttcctcccg tcgatgaaac caaacgcggt cgctatcagc aaatcaatct
     8101 gcgtgacgct tacgttgatc acctgttcgg ttatatcaac gtcaaaaacc tcacgccgct
     8161 caagctggtg atcaactccg ggaacggcgc agcgggtccg gtggtggacg ccattgaagc
     8221 ccgctttaaa gccctcggcg cacctgtgga attaatcaaa gtgcacaaca cgccggacgg
     8281 caatttcccc aacggtattc ctaacccgct actgccggaa tgtcgcgacg acacccgcaa
     8341 tgcggtcatc aaacacggtg cggatatggg cattgctttt gatggcgatt ttgaccgctg
     8401 cttcctgttt gacgaaaaag ggcagtttat tgagggttac tacattgtcg gtctgctggc
     8461 agaagcgttc ctcgaaaaaa atcccggcgc gaagatcatc catgatccgc gtctctcctg
     8521 gaacaccgtt gatgtggtga ctgctgcagg cggcacgccg gtaatgtcga aaaccggaca
     8581 cgcctttatt aaagaacgta tgcgcaagga agacgccatc tacggtggcg aaatgagcgc
     8641 ccaccactat ttccgtgatt tcgcttactg cgacagcggc atgatcccgt ggctgctggt
     8701 cgccgaactg gtgtgcctga aaggaaaaac gctgggcgaa ctggtgcgcg accggatggc
     8761 ggcgttcccg gcaagcggtg agataaattg tactgttcat aatcaaaaaa gtattattaa
     8821 taagatactt aatgaatttc aagctcaagc aaaaaatata gactatactg atgggataag
     8881 tatggaattc gaaaggtggc gttttaattt gagaagctcg aatacagagc cagtattacg
     8941 tttaaatgtt gaatccaaga aggataaaga tttaatggaa gaaaagacta aggaattatt
     9001 aagtagaata gtaaaataat aatatatagc atgtcaaata ctcttaattt gacatgctaa
     9061 aattaaaatc atgaatgtaa aatgtgacta cgagagttct aattcttttc aatatccact
     9121 acccagttta gtaaacaaaa aatgattcta tataaagaac gaagtgtaca ggcaaatgtt
     9181 attacaaaat taatggcgat atgtattgtt atatgttttg ccatgtttat attgttgctt
     9241 aagaatatgt taggcaataa cggatatgct acgctcttac atgttctggc tccgttctta
     9301 tctatctgtt taattgctgt aatgattttc ttttatataa aaagagcgtc atttatttcg
     9361 ctacaattga tgtatgcctt tgcattttca ttaattattg gcgtgccagg tatatatcta
     9421 ttgaatttta ggaaccctgt aaaaggtttt gaaatagtat gtttgtgggc tattttgctg
     9481 aatatagtgc tttatttttg ctcatacaat aacaaaacta taacacaaag aaaaaaagtt
     9541 aataaatatt ttgttgtaat atttgttatt gtgggtatat gtcaattgat taaaacaggg
     9601 ttatatttca tatttattgt taactctgga gttggacatt tggcaatata caccgaggga
     9661 gacgcattag tatctcaagt tccttttgtt atacgagcaa ttagtggtat gtcgttgatt
     9721 atgtctttgg ctacttttta ttatagagct tctacggggg tacgatggtt tagttttctt
     9781 ttgttagctt cggaactact tattggcatt agaagtaagt tttttttctc tctaataagt
     9841 ctaataattt tgtctttata ttgtaataaa gagttgataa aaaaaagatt tttaaagatc
     9901 tcgagactat tgtatttagt aattggattt gtgttatttt cacttgtctc atattataga
     9961 gaagggtatg atataaattt cgttaaatat atggttatag tactggactc actttcctct
    10021 actttagctg gcttacaaga tatattcgtt ttacctgatt ctcaaggttg gaatagatta
    10081 gatcctcatc taataataac gcaacttttc ccaataagtg ggttgggatt cttaaacagt
    10141 acacagatat ataaagagtt ttccgttatt gttcttggtg atatttcaag tgggatagct
    10201 ctgtcatcct cgggtatatt agaatcaact attttagatg ttgattttaa ttttttcatt
    10261 tacttatcct atttactgtt aatgctttct ttaattcaac attttctaaa cagtccaaaa
    10321 gtattcttaa attttatcgc aattgctatg cttccaggat ttttatattc aataagggga
    10381 gaattgattt tgccaattgc atatattatt aaatcttctc ctattataat tgtatcaaat
    10441 tttttggtct cagataaaaa tgcggatgag tgaacatgat tagagatatt ttcaatgatg
    10501 gcatatattc tctcatcaat aaaataggtt attttggtgc aaagacaata gctataatat
    10561 taattactag aactttaggt gttgacgaag gaagtcaatt tatatttctt attagtatca
    10621 tagaaataat gcgagtaata ggtgattttg gtgttgatgt gtatgtgatt agaaaatatg
    10681 gggacgtgcc taataaggaa aggcttttat gcactgtttt ttatcaaaaa attatagtcg
    10741 gctttgtctt tgcagcgata ctaatagtgt attgttcaat tttaaaatat aacgcgagca
    10801 tatacgttcc gttatctttg tcaataattt tttcattatt atttaatctt tccagtagct
    10861 atttccaatc cttaaacatt aataaggcta ttaccacatc aattatcagt gccagtattt
    10921 ttattggtgt attgttctca tctttttatt attttaatgt tgttgttaat gcttatcact
    10981 ttttagtgat tgaaatagtt tattgcatgt cagtatcttt tacgctaatt aataaaaccg
    11041 atctaaaaat aattaggcaa tttaagaagg ttgatatatc ggcaattcta tatttatacc
    11101 ggaatactta tagcattggt attacagcat taattgtaat tatctatagt agattggata
    11161 acatctattt aaaactttac gatgagcaaa gccttgctgt ttatggtcaa atttttagat
    11221 ttgttgatcc attggttatg atttcttctg ttttttcaac agtagcatat gcaaaattca
    11281 gcaattttaa gttacataat aacgaggcat ctataaaatc atttctttta atgatagtaa
    11341 tctatattct tgttagtttc cttatttact attatgtcct catctcaatt gggcaatggt
    11401 ttatattacc aaccagttta tttaagccat tgcttattgg ttttttgtgt atggcttcga
    11461 ttaagtgtat gaatggttca cttaccgcca tattacagtc tcaaggttta tataaaatag
    11521 gattgtatat ttcaatactt tgtattataa ttgcagtgcc gcttatgtat gtgtttgtaa
    11581 aacattatca tgcattaggg tgtatttatg cagtacttat tgttgagact gttagtttta
    11641 tatttttaag tgcaagcgta tattttttga cctctaatag gaatcataaa gatttaaatg
    11701 tcaaatagaa agaaattgac tgtattttca gggtaattag tctcttaatg ttacaatttc
    11761 acgtcacttt tttatgtttg acattttcgt tataaactta atattttgcc tgatccttat
    11821 tgtcttttat ggaacagccc ttatacaagc tgggtatttg ttatcaaaaa tagctaaata
    11881 attattatta acatagttat tctactaatc taaattttaa ccgtgctttt tacataagcc
    11941 gccacagcgg ttatcatccc tgacaggagt aaacaatgtc aaagcaacag atcggcgtag
    12001 tcggtatggc agtgatgggg cgcaacctag cgctcaacat cgaaagccgt ggttataccg
    12061 tctctatttt caaccgctcc cgtgagaaga cggaagaagt gattgccgaa aatccaggca
    12121 agaaactggt tccttactat acggtgaaag agtttgttga atctctggaa acgcctcgtc
    12181 gcatcctgtt aatggtgaaa gcaggtgcag gcacggatgc tgctattgat tccctcaagc
    12241 cgtacctcga taaaggtgac atcatcattg atggtggtaa caccttcttc caggacacca
    12301 ttcgtcgtaa ccgtgagctt tctgccgaag gctttaactt catcggtacc ggtgtttccg
    12361 gcggagaaga aggcgcgctg aaaggtcctt ccattatgcc tggtgggcag aaagaagcct
    12421 atgaactggt tgcgccgatc ctgaccaaaa tcgccgcagt agctgaagac ggtgagccat
    12481 gcgttaccta tattggtgcc gatggcgcag gtcactatgt gaagatggtt cacaacggta
    12541 ttgaatacgg cgatatgcag ctgattgctg aagcctattc tctgctaaaa ggtggcctga
    12601 acctcaccaa cgaagaactg gcgcagactt ttaccgagtg gaataacggt gaactgagca
    12661 gctacctgat cgacatcacc aaagatatct tcaccaaaaa agatgaagac ggtaactacc
    12721 tggttgatgt gattctggat gaagcagcaa acaaaggtac gggcaaatgg accagccaga
    12781 gtgcgctgga tctcggcgaa ccgctgtcgc tgataaccga gtctgtgttt gcacgttata
    12841 tctcttctct gaaagatcag cgcgttgccg cgtctaaagt tctctctggc ccgcaagcgc
    12901 agccagcagg cgacaaggct gagttcatcg aaaaagttcg ccgtgcgctg tatctgggca
    12961 aaatcgtttc ttacgctcag ggcttctctc agctacgcgc cgcgtctgaa gagtacaact
    13021 gggatctgaa ctacggcgaa atcgcgaaga ttttccgtgc tggctgcatc atccgtgcgc
    13081 agttcctgca gaaaatcacc gatgcttatg ccgaagatcc gcagatcgct aacctgctgc
    13141 tggctccgta cttcaagcaa attgctgatg actaccagca ggcgctgcgt gatgtcgttg
    13201 cttatgcagt acaaaacggt atcccggttc cgaccttcgc cgctgcggtt gcctattacg
    13261 atagctaccg tgccgctgtt ctgcctgcga acctgatcca ggcacagcgt gactatttcg
    13321 gtgcacatac ttataagcgc attgataaag aaggtgtgtt ccatactgaa tggctggatt
    13381 aatctgattt aaccaacaat aaaattgagg cccggcgtat attgcaccgg gctttttttt
    13441 tgccaattat cttatagact aaatttcact gcttaatatt aacttaataa atatcagcta
    13501 ttcttataaa gaaattctga attgtttttc gttgcgttga ccatcttaca acgtagcgtt
    13561 aaaactttta actcttatca ggatgctaaa aacatcatga ttcacagtta agttaattct
    13621 gagagcatga aatgaaaatt accatttccg gtactggcta tgtaggcttg tcaaacgggc
    13681 ttctaatcgc acaaaatcat gaggttgtgg cattagatat tttaccgtca cgcgttgcta
    13741 tgctgaatga tcggatatct cctattgttg ataaggaaat tcagcagttt ttgcaatcag
    13801 ataaaataca ctttaatgcc acattagata aaaatgaagc ctaccgggat gctgattatg
    13861 tcatcatcgc cactccaacc gactatgatc ctaaaactaa ttatttcaat acatccagtg
    13921 tagaatcagt aattaaagac gtagttgaga taaatcctta tgcggttatg gtcatcaaat
    13981 caacggttcc cgttggtttt accgcagcga tgcataagaa atatcgtact gaaaatatta
    14041 ttttctctcc ggaatttctc cgtgaaggta aagcccttta cgataatctc catccttcac
    14101 gtattgtcat aggtgagcgt tcagaacgcg ctgaacgttt tgctgcgttg ttacaggaag
    14161 gggcgattaa gcaaaatatc ccgaccctgt ttaccgactc cactgaagca gaagcgataa
    14221 aacttttcgc taatacctat ctggcgatgc gcgtagcgta ctttaatgaa ctggatagct
    14281 atgcagaaag tttaggtctg aatactcgcc agattatcga aggcgtttgt ctcgatccgc
    14341 gtattggcaa ccattacaac aatccgtcgt ttggttatgg tggttattgt ttgccgaaag
    14401 ataccaaaca gttactggcg aattatcagt ctgtgccgaa taacctgatt tcggcaattg
    14461 tcgatgctaa ccgcacgcgt aaagatttta ttgccgatgc cattttgtca cgcaagccgc
    14521 aagtggtggg tgtttatcgt ctgattatga agagcggttc agacaacttc cgcgcgtctt
    14581 ccattcaggg gattatgaaa cgtatcaagg cgaaaggcgt tgaagtcatc atttacgaac
    14641 cggtgatgaa agaagactca ttcttcaact ctcgcctgga acgagatctc gccactttca
    14701 aacaacaagc tgacgtcatt atctccaatc gtatggcaga agagcttaag gatgtggcag
    14761 ataaggtata cacccgcgat ctctttggca gcgactaaca tcttgttatc agggctattt
    14821 acgccctgat tgtcttttgt tacttcctca ataattcatt atttttatca cttatcctat
    14881 agcattcacg aggattatcg ctaaactatg cggacttgga aatttccgtc agttagggta
    14941 atgatgagag tagaaaataa taatgtttct gggcaaaacc atgacccgga acagattgat
    15001 ttgattgatt tactaatgca gttgtggcgt ggcaagatga caatcatcat ctctgtcatt
    15061 gttgctattg ccctggctat agggtatctg gccgttgcca aagaaaagtg gacttccaca
    15121 gctatcgtta ctcaacctga tgtggggcaa atcgctggct ataacaatgc catgaatgtt
    15181 atctatggtc aggctgcacc gaaagtgtca gatttgcagg agacgttgat tggtcgcttc
    15241 agttctgcct tctctgcatt agcagaaacg ctggataatc aggaagaacc agaaaaactt
    15301 accatcgaac cttctgttaa gaaccagcaa ttaccattga ctgtttctta tgttgggcaa
    15361 actgcagagg gcgcacaaat gaagttggcc caatacattc agcaagttga cgataaagtg
    15421 aatcaagagc tagaaaagga tctcaaagac aacattgctc tgggacggaa aaacttgcag
    15481 gactctttaa gaacgcagga agtggtcgcg caggagcaga aagatctgcg tatccgtcag
    15541 attcaggaag cattgcagta tgcgaatcag gcgcaggtga caaaaccaca ggttcagcag
    15601 actgaagatg tgacgcaaga tacgttgttc cttcttggga gcgaagcact ggagtcgatg
    15661 attaagcatg aggcgacccg tccgttggtg ttctcaccaa actactatca gactcgtcaa
    15721 aacctgctgg atatcgaaag cttaaaggtt gatgatcttg atattcatgc ttaccgttat
    15781 gtgatgaaac cgacgttacc tattcgtcgc gatagcccga aaaaggcaat taccttgatt
    15841 ctggcagtgc tactgggtgg catggttggc gcggggattg tgctggggcg taacgctcta
    15901 cgcaattaca acgcgaaata atctttgcag ttttaaagaa aatgggcagg gtggtgacac
    15961 cttgcccgtt ttttttgccg gatgcgatgc tggcgcatct tatccagcca aaatgtgcag
    16021 agcgaatagt aggcccgata agcttgcgca tcgggcaatg gctacggatg cgactacaac
    16081 atcgcatccg gtcagtccgc aactcactga tgccgtttgc gcaggttctc aatcaccgtc
    16141 gttaaatcca gcccctgatc ctgcaacagc accagcaggt gatacatcaa gtcagatgcc
    16201 tcgttggtca gctcaaagcg gtcatgtacc gttgctgcca gcgcagtttc caccccttct
    16261 tcgcccactt tctgcgcgat gcgtttggtg ccgctggcat acagtttggc tgtgtaagag
    16321 gtttccgggt cggcagattt acgttcggcg agcagttgtt ccagttgata caggaacagc
    16381 cactggtgag cggtgttgcc gaagcaactg ctggtgcctt tgtggcaggt cgggccgatg
    16441 ggattcgcca gcactaataa agtgtcgttg tcgcagtccg acgcaatact cactacgttg
    16501 aggaaattac ccgacgtttc gcctttggtc cacagtcgct gtttagtgcg cgagaagaag
    16561 gtaactttgc cactttcgat ggttttgtcc agagcttccg ggttcatgta gcccagcatt
    16621 aacacttcac cggataccgc gtgttgcaca atcaccggca tcagtccgtc ggttttttcc
    16681 cagtccagtt cgcgacgttg ttgttctgtt aacatatcct gatctccacg ccctgtgttg
    16741 ccaggtacgc ttttaattca ccaatattga ttatttgttt gtggaatacg gaagctgcca
    16801 gcgcgccgtc aacgtcggca tcgcggaagg cttccaggaa gtgttccatg gtgcccgcgc
    16861 caccggaggc aattagcggg acgtggcaag cttcacgcac ttttttcagt tgttcgaggt
    16921 cgtaaccgtt acgcacgccg tcctgattca tcatgttgag gacgatttct ccggcaccgc
    16981 gtttttgcac ttcctgtacc cagtcgagag tttcccactg agtaacgcgg gtgcggcttt
    17041 catcgccggt atattgattc acatgatatt taccggtttc agcgtcgtac caggtatcaa
    17101 taccgaccac aatacactgc acgccaaagc gatccgccag gcgagtgatt aatgtcggct
    17161 ccgccagcgc aggggagttg atggaaattt tatctgcgcc aaaggaaaga attttcgcgg
    17221 catcttccag agacttaatc ccacccgc
//
