LOCUS       AB812075               17202 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: E54071.
ACCESSION   AB812075
VERSION     AB812075.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 17202)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..17202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E54071"
                     /db_xref="taxon:562"
                     /note="serogroup:O178"
     gene            <1..911
                     /gene="wcaM"
     CDS             <1..911
                     /gene="wcaM"
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01941.1"
                     /translation="ANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDIL
                     ISDHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHV
                     ENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGV
                     IKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEVKRASLELHN
                     KPQHLFMRNIKVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQS
                     SVDIDRINHHIVNVEKINFRLPERRE"
     gene            1069..2064
                     /gene="gne"
     CDS             1069..2064
                     /gene="gnu"
                     /note="O178_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAQ01942.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            2307..3200
                     /gene="galF"
     CDS             2307..3200
                     /gene="galF"
                     /note="O178_02"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01943.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     CDS             3524..4126
                     /note="O178_03"
                     /gene="wbuN"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydrolase"
                     /protein_id="BAQ01944.1"
                     /translation="MKKILLIDICGTITEKNTTLDFISYIGNKPSILKIIIGRICWRL
                     FKKDTIRKWYLNELKGLSYTELVNLAREYVNQLNYDPQIICLINKHRGECDLYFVSAT
                     LDFIAKAMTEKFNVSGFFASELQFEDGICSGKLKIDLLDNKNSVIAHMFNGDNNVCII
                     SDNYGDYSVMEKCCSSWAVVRNKRALHYWSKKSVNIINRM"
     CDS             4129..4911
                     /note="O178_04"
                     /gene="wbuO"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ01945.1"
                     /translation="MYIFICPFAYTYNTRLRSLISLLSWGVIYFLLLLLFVMVQNDGI
                     NYTEIMIFCVSIIIVYNNYEIGYIINDTETIKKEKKPTMRLGRNALDIYESNKLLIYG
                     IRVCLSIVLTLFLIKILKVQGYYIISAWLILPVFIIYNSIRNRLNLALHFTLVTLRYC
                     SPILIVLQPEHIINVILNVSFIFPLPNLIERCSEPRFDFSIFHKVKSNHNKWRVFYYF
                     MATTITGLALYTKMLSEYVVFICCLYMLVYRVLSPLLIQKKN"
     CDS             5430..6500
                     /note="O178_05"
                     /gene="wetO"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01946.1"
                     /translation="MKKVTFLINSLTQGGAEKIALQLYLNLSNSVNVINFVTLTNEDF
                     YKKEIKKHVINHSNKITKTTKFMSLAKYFRGNNDVVLCFSLDLACYLYILRVLNIFKG
                     EVICRFINNPDNEIGTSLINKIKKRFLFYVLKKCDLVICQSDAMKDLLISKYEFNNNK
                     TVRIYNPISIANNNESSFEQTDKEILKLLFVGRLSEQKNLDDIIRISQLLRDKGILFL
                     WKVVGKGPLENTFKDLIREHNFQESIVMCGSSHDIENYYKWADITTLVSHYEGLPNVL
                     LESISHRTPCVSYDCPTGPSEIIIHEKNGVLVPQYDVDNFYQSLIRVKELNLKNDNIE
                     NTLMKFSEKKVCDEYYKILFKR"
     gene            6506..7735
                     /gene="wzx"
     CDS             6506..7735
                     /gene="wzx"
                     /note="O178_06"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01947.1"
                     /translation="MKKVLGNFLNLTLLQLSTMVAQILTYPYLNRTLGSEIYGEIILQ
                     QIIVFHLQMIVFFGTDLSVVRLAAKYSTSYKKLKILVSKIILGRFYISLIISLSYVIY
                     IYCSGLNDWFYFFVFSIFEAVITLRWFYHGIQQLNKFTLPFCLVRFLGVLLLFIFVNT
                     QDDWYKVIIITVLSSLLSVIVSFYSYYVKYNLTYIKFRQIYIIFSDSFSLFATNIVSV
                     IKDRSGGIFISYFMGTSSLVYYDFCMKIVGVISSITSSISASLYPMFSGNYNHSTFKR
                     YNLLILLFSIIPFIITLLADKYVIFLIDYIFSINIFDIKLLLPVFGFMIFVRSHGYFI
                     GLCYLMALNYKKSYASSLIYSGMFYLFFMSGMIFLGYKNILTLAVGLSLSLLLEYFHR
                     IYKFISTRKNANESHAE"
     gene            7716..8813
                     /gene="wzy"
     CDS             7716..8813
                     /gene="wzy"
                     /note="O178_07"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01948.1"
                     /translation="MNLTLNNFMAYILAITMVIQSYSKVFFASDTIFQNIFFYALVFF
                     SILIVSISKFRLIDIFLIITSLIIYLAFGNGFALKLFLVSLAVRCLDINKLLRCYLLL
                     ATIAFITVILFNVGGDNSLIYFKGDGVFRIARETLGFDNPNKPFYYLLPIFCCFVFLY
                     FKRYPISCITAIIIVTYAVYIKTLTTTGLFSNALLVLMLLIYYFLPNKTNRILGNPLL
                     ITCSIIVLYFASFFIAFTYHSDSNVNFFLSHRPEYWYEIIRTTNIYLLIFGQALDLQL
                     VPLDNSYIHSVIYMGAFFCIIMIFCYWLGLTRAKLRGVNISIISILCIYVFFYSFGET
                     LLVEPTLNITFIIIFNYIRDHNKYESIDLQP"
     CDS             8788..9939
                     /note="O178_08"
                     /gene="wetP"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01949.1"
                     /translation="MKVLIFSHEYPPIGGGAGVVANQLIDHFCEDSAIEQIDLLTRFS
                     HQYNINKRVHNIFMVNIHNIIWPLEYFFYLKKTINLSLYDLIICNDSISIYIAGMLFS
                     SRELEKTVCFLHGSEPEFIYQNNNIQKRLLNLRFFFHRALIKCSFIGAHSEFMKEKFL
                     KNLPKKLTINSDKIIPLYFGYDASLFNTDNKIENKNTIRNKYKIKESDILLLTVSRVE
                     EKKGFVKMLNTFEIMKSMNENLKWMIVGDGGFLESLKEIAFRKGIFDSLIIVGKIPRS
                     ELCMFYNSADIFWLLSEYQEAFGLVYIESQACGVPAIGYNNSGVREAVVDGVTGYLIN
                     NLDEMLDIVVTKKFQIITNESLYDFSKKFDSKQCYLKILKAFNEKVINE"
     CDS             9932..10771
                     /note="O178_09"
                     /gene="wbwC"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01950.1"
                     /translation="MSDVIMHDKKTEEFSVLMSLYCNEKPEFLNQCLISIHEQSVKPN
                     EIIIVYDGYIPAELDNVVIEWSKVLPVKVCKLHTNMGLGDALNFGIKHCNNELIARMD
                     TDDICAKDRFKLQLQYFNENPTLTLIGGGIEEYDEEMQVLRGTRFTKEKHADIVKYAC
                     FKNPFNHMTVMFKKKDIQSVGGYKKHLLMEDYNLWLRLLNNGYKTYNLPEILVYARTG
                     INMVRKRRGMSYFKSEIQLFKLKRMLNCNSYSKNCVIFLIRVLPRFLPVSVLSIVYKI
                     MRK"
     gene            10883..12289
                     /gene="gnd"
     CDS             10883..12289
                     /gene="gnd"
                     /note="O178_10"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01951.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEEGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            12538..13704
                     /gene="ugd"
     CDS             12538..13704
                     /gene="ugd"
                     /note="O178_11"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01952.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKELNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPDAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSDRAQRFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     CDS             complement(13770..14774)
                     /note="O178_12"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide sugar epimerase"
                     /protein_id="BAQ01953.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFVDERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYSLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPHWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            15126..16160
                     /gene="wzz"
     CDS             15126..16160
                     /gene="wzz"
                     /note="O178_13"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01954.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAQYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(16257..16868)
                     /gene="hisI"
     CDS             complement(16257..16868)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01955.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     5225 a   3048 c   3544 g   5385 t
ORIGIN
        1 acgctaatta cgccatctta cggcagggat ttcataacca gataatcggt gccaacatca
       61 ccaattgtaa gttcagcgac ttacaggggg acgctattga atggaacgtg gcaattaacg
      121 acagtgatat tttgatctcc gaccatgtca tcgagcgcat caactgtact aacggcaaaa
      181 tcaactgggg cattggcata ggccttgcgg gaagcactta cgataataat tacccggaag
      241 atcaggcagt gaaaaacttt gtcgtggcga atatcacggg atcggattgt cggcagttga
      301 tccatgttga aaatggtaaa cattttgtta ttcgtaatat caaagcccgc aatatcacgc
      361 cggatttcag taagaaagca ggtattgata acgcgacagt cgctatttac ggttgtgaca
      421 atttcgtgat tgataatatt gaaatgatta atagcgccgg gatgttaatc ggctatgggg
      481 taattaaagg caaatatctc tcgataccgc aaaatttccg agtgaataat attcaactgg
      541 ataataccca ccttgcttac aaattgcgcg gtatccaaat ctctgccggg aatgccgtct
      601 cctttgtggc actgactaac attgaggtga agcgtgcgtc gctggagtta cacaacaaac
      661 cgcaacatct ttttatgcgt aatatcaaag tgatgcagga atcctcagtt ggacccgcat
      721 tgagcatgaa cttcgacatg cgcaaagacg ttcgcggcgt ctttatggcg aaaaaagaaa
      781 cactgctgtc tcttgcaaat gttcatgcgg tgaatgaaag agggcaaagc tccgtcgata
      841 tcgacaggat aaatcaccat attgttaatg tggaaaagat taactttaga ttgccggaac
      901 ggagggagta gatttgcgac cattcataaa cttactggat ataatgttct aaaagattat
      961 aatcctcaca ctaactgtta aagctggggc tgggcagcat tgactactca gggaaggtac
     1021 tcatctttat cttgtgtttc ttacgtaaca accagactaa agtgatttat gaacgataac
     1081 gttttgctca taggagcttc cggattcgta ggaacccgac tacttgaaac ggcaattgct
     1141 gactttaata tcaagaacct ggacaaacag cagagccact tttatccaga aatcacacag
     1201 attggcgatg ttcgtgatca acaggcactc gaccaggcgt tagccggttt tgacactgtt
     1261 gtactactgg cagcggaaca ccgcgatgac gtcagcccta cttctctcta ttatgatgtc
     1321 aacgttcagg gtacccgcaa tgtgctggcg gccatggaaa aaaatggcgt taaaaatatc
     1381 atctttacca gttccgttgc tgtttatggt ttgaacaaac acaaccctga cgaaaaccat
     1441 ccacacgacc ctttcaacca ctacggcaaa agtaagtggc aggcagagga agtgctgcgt
     1501 gaatggtata acaaagcacc aacagaacgt tcattaacca tcatccgtcc taccgttatc
     1561 ttcggtgaac gcaaccgcgg taacgtctat aacttgctga aacagatcgc tggcggcaag
     1621 tttatgatgg tgggcgcagg gactaactat aagtccatgg cttatgttgg aaacattgtt
     1681 gagtttatca agtacaaact gaagaatgtt gccgcaggtt atgaggttta taattacgtt
     1741 gataagccag atctgaacat gaaccagttg gttgctgaag ttgaacaaag tctgaacaaa
     1801 aagatccctt ctatgcactt gccttaccca ctaggaatgt tgggtggata ttgctttgat
     1861 atcctgagca aaattacggg caaaaaatac gctgtcagct ctgtgcgcgt gaaaaaattc
     1921 tgcgcaacaa cacagtttga cgcaacgaaa gtgcattctt caggttttgt ggcaccgtat
     1981 acgctgtcgc aaggtctgga tcgaactctg cagtatgaat tcgttcatgc caaaaaagac
     2041 gacataacgt ttgtttctga gtaatgcaga cgagtcagag aaaaaaggac caggttggac
     2101 tacgattatt cggaaagagg tactgattaa attatcagta tgcggaattc cgagtaatcc
     2161 gagtaaccca gatgctgatg gtgtgtagtg ttatcctaag gtttaagcaa agttatgcgg
     2221 aactaagctt tggtatatag ccataacgtt tggctaacct atggcaataa tttacgcact
     2281 atcagccact tagaggtagg aaaagaatga cgaatttaaa agcagttata ccggtagcag
     2341 gtcttgggat gcatatgttg cctgccacta aggcgattcc caaagagatg ctaccgatcg
     2401 tcgacaagcc aatgattcag tacattgttg acgagattgt ggctgcaggg atcaaagaaa
     2461 tcctcctggt aactcacgcg tccaagaacg cagtcgaaaa ccacttcgac acctcttatg
     2521 aattagaatc tctcctggaa cagcgcgtga agcgtcaact gttggcggaa gtacaatcta
     2581 tctgtccgcc gggcgtgacc attatgaacg tgcgtcaggg tgaaccttta ggtttgggcc
     2641 actccatttt gtgtgcacgc cccgccattg gcgacaaccc atttgtcgtg gtgctgccgg
     2701 acgttgttat cgatgatgcc agcgccgacc cgctgcgcta caaccttgct gccatgattg
     2761 cgcgtttcaa tgaaacgggc cgcagccagg tgctggcaaa acgtatgccg ggtgacctct
     2821 ctgaatactc tgtcatccag accaaagagc cgctggaccg tgaaggcaaa gtcagccgca
     2881 ttgttgaatt tatcgaaaaa ccggatcagc cgcagacgct ggactcagac atcatggccg
     2941 taggtcgtta tgtgctttct gccgatattt ggccggaact ggagcgcact cagcctggtg
     3001 catggggacg tattcagctg actgatgcta ttgccgaact ggcgaaaaaa cagtccgttg
     3061 atgcaatgct gatgactggt gacagctacg actgcggtaa aaaaatgggc tatatgcagg
     3121 cgtttgtgaa gtatggactg cgcaacctga aagaaggggc gaagttccgt aaaggcattg
     3181 agaagctgtt aagcgaataa tgaaaatctg accggatgta acggttgata agaaaattat
     3241 aacggcagtg aggattcgtg gcaaaagtaa tttgttgcga atattcctgc cgttgtttta
     3301 tataaacaat cagaataaca acgagttagc aataggattt tagtcaaagt tttccaggat
     3361 tttccttgtt tccagaacgg attggtaaga caattagcgt ttgaattttt cgagttaagc
     3421 gcgagtgggt aacgctcgtc acatcgtagg catgcatgca gtgctctggt agctgtaaag
     3481 ccaggggcgg tagcgtggaa attacgagag ttatagggtt taaatgaaaa aaattttatt
     3541 aattgacata tgtggaacaa taactgagaa aaataccaca ttagatttta tttcatacat
     3601 tggaaataag ccaagtattc taaaaattat tatcggacgt atttgctggc gcttatttaa
     3661 aaaagatact ataagaaaat ggtatctcaa tgaattgaaa ggattaagtt atacggagtt
     3721 agttaattta gcaagggaat atgtgaacca gttgaattat gacccccaga ttatatgttt
     3781 aataaataaa catagaggcg agtgtgactt atattttgtg tctgctacgt tagattttat
     3841 agcaaaagca atgacggaaa aatttaatgt atctggtttt tttgcatcgg aattacaatt
     3901 tgaagatggt atttgttctg gaaaattaaa aatagatttg cttgataata aaaatagcgt
     3961 gattgcacat atgtttaatg gtgataataa tgtttgtata attagcgata attatggcga
     4021 ttatagcgtt atggaaaaat gttgtagttc atgggctgta gtgagaaata aaagagcatt
     4081 acattattgg agtaaaaaat cggttaacat catcaacagg atgtaaatat gtatattttc
     4141 atatgtccgt ttgcttatac ttataatact aggttaagat cattaatttc actactgtca
     4201 tggggggtaa tttattttct tttgttatta ctgtttgtta tggtacaaaa tgatggaatt
     4261 aattacacag aaattatgat tttttgtgtt tctattataa ttgtttataa taattatgaa
     4321 attggctata ttattaatga tactgaaacg attaagaaag aaaaaaaacc aacaatgcgt
     4381 cttggaagaa atgcactaga tatatatgaa tctaataaat tattaatata tggaattaga
     4441 gtgtgtttat caattgtatt aacattgttt ttaattaaga ttcttaaagt gcaaggttat
     4501 tatataatat cagcatggct aatccttcct gtttttatta tatataattc tataaggaac
     4561 agattaaatt tagctcttca ttttacattg gttactttaa gatattgttc acctatatta
     4621 atagtattac agccagaaca tataataaac gttattctca atgtttcttt tattttccct
     4681 ttaccaaatc taattgaaag atgttcagaa ccaagatttg atttttcgat ttttcataaa
     4741 gtaaaatcaa atcataacaa atggagagtc ttttattatt ttatggcgac cactattact
     4801 ggattagccc tttatacgaa aatgttatct gaatacgtgg ttttcatatg ttgtttgtat
     4861 atgctggtat atagagtttt atcaccctta ttgattcaga aaaaaaattg actataagat
     4921 tatataaata ataaagtacc ttatgctaag gagattgaac tgaagaaggt gatttaaaac
     4981 cttctatact atactttatt aaatactaag ataacgcgta aatggttaaa atgacagtga
     5041 ttaaaatgat aaatatgtta tctttcaatt aattcctaat ttgaattcta ttcagtacct
     5101 aaatattatc ttcatttaaa ttcaatggat ggttttggaa atttaatatg acttattctg
     5161 atttctagaa aaataataag aattgttatt atatcttggg gggattaaag aattcatatt
     5221 agtaatcaat gagaataaaa taaaagaggg aattaattta cgttattttg ataaacattt
     5281 ttcacaatta aaatttttaa atgcgttaag tatttatcaa tacaaaatat aaaagggtta
     5341 tcccgaaagc tactgctcac gttagtgttg agggaatata ttttttactc ttacaaagag
     5401 tgatgaactt cggttaaaag tgaatataaa tgaaaaaagt tacttttctt attaatagct
     5461 taacacaagg tggagcagaa aaaattgcat tgcaacttta cctaaatttg agtaattcag
     5521 ttaatgttat taattttgtt acattgacaa atgaagattt ttataagaag gaaataaaaa
     5581 aacatgtgat aaatcatagc aataaaataa ctaaaacaac aaaatttatg tcattagcaa
     5641 agtattttag aggtaataat gacgttgtct tatgtttttc attagatctt gcttgttatt
     5701 tatatatcct tcgcgttcta aatattttta aaggagaggt gatttgtaga ttcataaata
     5761 atcctgacaa tgaaatagga acttcattaa ttaataaaat aaaaaaaaga tttctttttt
     5821 atgtgttaaa aaaatgcgat cttgttattt gccaatcaga tgctatgaag gatcttttga
     5881 taagcaaata tgaattcaat aataataaaa cagtaaggat ttataatcca atatctattg
     5941 caaataataa tgaatctagt ttcgagcaaa ctgataaaga aattttaaaa ttactttttg
     6001 ttggaaggct tagcgaacaa aaaaatttag atgatataat tcgtatttct caactattga
     6061 gagataaagg aatattattt ttgtggaagg ttgtcggaaa agggccgctg gaaaacacat
     6121 tcaaggattt gataagagaa cacaatttcc aagagagtat cgtaatgtgt ggttcgtcac
     6181 atgatataga aaactactat aagtgggcag atatcacaac tttagtttct cattatgaag
     6241 ggcttccgaa tgtattgtta gaatccatct cgcatagaac gccatgcgta agttatgact
     6301 gtcccactgg tccttctgaa ataattattc atgaaaaaaa tggtgtttta gtacctcaat
     6361 acgatgtgga caatttttat caatctctta ttcgagttaa agagctaaat ttaaaaaatg
     6421 ataatattga gaatacatta atgaaatttt cggagaaaaa agtttgtgat gaatactata
     6481 aaattctttt taagagataa tcagattgaa gaaagtatta ggaaatttct taaatttgac
     6541 acttttacag ttgtcaacaa tggttgcaca aatccttact tacccttact taaataggac
     6601 tctgggcagt gagatatatg gggaaataat tcttcagcag ataatagttt ttcatcttca
     6661 aatgatagtt ttttttggta cagacctgtc cgtagtgagg ttggcagcaa aatatagtac
     6721 aagctataaa aaactgaaaa ttttggtgag taaaataatc cttggtcgat tttatatttc
     6781 tttaatcatt tctttatctt acgttattta tatttattgt agcggtttaa atgattggtt
     6841 ttattttttt gtattttcta tttttgaagc agttattact ttgagatggt tttatcatgg
     6901 tattcagcaa cttaataaat ttaccttacc tttttgttta gtcagatttt taggagtgtt
     6961 gcttctattt atctttgtta atactcagga tgattggtat aaggttataa taataactgt
     7021 attgtcgtca ttactcagtg ttatagtatc tttctattcc tactatgtta aatataatct
     7081 tacttatatt aagtttcgtc aaatatatat tatattttct gattcatttt cgctctttgc
     7141 tacaaacatt gtttcggtga ttaaggaccg atctggaggt atttttatca gctattttat
     7201 gggaacatct tcactagttt attacgattt ttgcatgaaa attgttggtg taatttcaag
     7261 tataacttct agcatatcag cttctttgta cccgatgttc tcaggaaatt ataaccattc
     7321 aacattcaaa agatataatt tactaatatt gttgttttcg ataattccat ttattattac
     7381 tctgctcgca gataaatatg tgattttttt gatagattat atatttagta ttaatatatt
     7441 tgatattaaa ttattgctgc cagtatttgg atttatgatt tttgtaagat ctcatggtta
     7501 cttcataggc ctctgttatc taatggcatt aaactataaa aagagttatg cttcatcatt
     7561 gatttattcg ggtatgtttt atttgttttt tatgagcggt atgatttttt taggctataa
     7621 aaatatttta acacttgctg tagggttatc attatcattg ttactagagt attttcatcg
     7681 gatatataaa tttattagta caaggaaaaa tgccaatgaa tctcacgctg aataatttta
     7741 tggcatatat actcgccatt acaatggtaa ttcaatctta tagtaaggtt ttctttgcat
     7801 ctgatactat ctttcagaat atcttttttt atgctttagt gtttttttca atattaattg
     7861 taagtatatc aaagttcaga ttgattgata tatttttgat tattacatca ttaataattt
     7921 atttagcatt cggtaacggc tttgcattaa agcttttctt agtttcatta gcagtacgtt
     7981 gtttagatat aaacaaattg ctccgttgtt atctcttact agcgactatt gcatttataa
     8041 cagttatatt attcaatgtg ggaggtgata actctttaat ttattttaaa ggagatggtg
     8101 tatttagaat tgcaagggaa acattaggtt ttgataatcc taacaaacca ttttattatc
     8161 ttttgccgat attttgttgt tttgtttttc tatatttcaa acgttatcca atatcatgca
     8221 taacagctat tataattgtt acatatgcag tatatataaa aacactaaca actacaggac
     8281 ttttcagtaa tgctttgctt gttttaatgt tgttgatata ttattttctt cccaataaaa
     8341 caaatcgaat attaggtaat cctcttttaa ttacttgctc aattattgta ttatattttg
     8401 ctagtttttt tattgccttc acttatcatt ctgatagtaa tgtgaatttc tttctttctc
     8461 acaggccaga atattggtat gagataatta gaacaacaaa tatttatttg ctaatatttg
     8521 gacaagcatt agatcttcaa ttggttccgt tagataattc ctatatacat tctgtcattt
     8581 atatgggggc gtttttctgt attataatga ttttttgcta ttggctgggg ttaaccagag
     8641 ctaaactcag aggggtaaac atttcaatta taagtatatt atgcatatat gtgttttttt
     8701 attcttttgg tgagactttg ttagttgagc caacactcaa catcacattt attattatat
     8761 ttaactatat acgggatcat aataaatatg aaagtattga tcttcagcca tgagtatcca
     8821 cctataggag gaggggctgg tgtagtagct aatcaactaa tcgaccattt ttgtgaagac
     8881 agcgcgatcg aacaaattga tctgcttact cgtttttcgc atcagtacaa tattaataag
     8941 agagtgcata atatttttat ggttaacatc cataatatta tatggcctct agagtacttt
     9001 ttttatctta agaaaactat aaatctttct ctttatgatc ttattatctg taatgactcc
     9061 atatctatat atattgcagg tatgctgttc tctagtagag aactagaaaa aaccgtatgt
     9121 tttttacatg gttctgaacc tgagtttata tatcaaaata ataatatcca aaaaaggtta
     9181 cttaatttaa ggtttttttt tcacagagct ctaattaagt gtagctttat cggagctcat
     9241 agtgaattta tgaaagaaaa attcctgaag aacttgccta aaaaattaac tattaattca
     9301 gataaaataa tccccttata ctttggctat gatgcatcat tgttcaatac tgataataaa
     9361 atagaaaata aaaatacaat tcgtaataaa tataaaataa aagaaagtga tatattatta
     9421 cttactgttt caagggtgga agaaaaaaag ggatttgtaa aaatgttaaa tacttttgaa
     9481 ataatgaaat ccatgaatga gaatctaaaa tggatgatag ttggggatgg gggattcctt
     9541 gaaagtttga aagaaattgc atttaggaaa gggatttttg attcactcat tatcgtaggt
     9601 aaaattccaa ggagtgagtt gtgcatgttt tataattctg cagatatttt ttggttatta
     9661 tcagaatacc aagaggcttt tggtttggta tatattgaat ctcaggcatg tggggtacct
     9721 gccattggct ataataattc aggagtaaga gaggctgttg ttgatggagt tacggggtat
     9781 ttaattaata atcttgatga aatgttggat atcgtagtga caaagaaatt ccaaattata
     9841 actaatgaat ccctatatga tttttcaaaa aaattcgact caaaacaatg ttacttaaaa
     9901 atactaaaag cgtttaacga gaaagttatc aatgagtgat gttataatgc acgataaaaa
     9961 aactgaggaa ttctcagtat taatgtcttt atattgtaac gaaaaacctg aatttttaaa
    10021 tcaatgctta ataagtatcc atgagcaatc tgtcaagcct aatgagatca tcattgttta
    10081 tgatggctat ataccggctg aattagataa tgtcgtaata gagtggagta aagttttacc
    10141 agtaaaagtt tgtaagttac acacaaatat ggggcttggc gatgcactta actttggtat
    10201 caaacattgt aataatgaat tgatcgcaag aatggatact gacgatattt gtgctaagga
    10261 tagattcaaa ttgcaactgc aatatttcaa tgaaaaccct actcttactt taatcggggg
    10321 aggtattgaa gaatatgatg aggaaatgca agtcctgaga ggaacgcgtt ttacaaaaga
    10381 aaaacatgct gatattgtca aatatgcttg cttcaaaaac ccattcaatc atatgactgt
    10441 aatgtttaag aaaaaagata ttcagtctgt cggtggatat aaaaaacatc tattaatgga
    10501 agattataat ctttggttgc gtcttttaaa taatgggtat aaaacatata acttaccaga
    10561 aatacttgtt tacgcgcgaa cgggtattaa catggttaga aaacgtagag gtatgagtta
    10621 ttttaaaagt gaaattcagc tttttaaact gaaaaggatg ttgaactgta attcatatag
    10681 taaaaactgt gttatctttt taattagagt actcccacga ttccttccgg tatctgtact
    10741 ttcaattgta tataaaataa tgcgaaagtg acaaacattt ttttataaag agaaaattaa
    10801 ttgcttcttc cttttttgaa aacaataaat attgtatatt actgttcttt tagaactact
    10861 tccccgcaga caggagtaaa caatgtcaaa gcaacagatc ggcgtcgtcg gtatggcagt
    10921 gatggggcgc aaccttgcgc tcaacatcga aagccgtggt tataccgtct ctattttcaa
    10981 ccgttcccgt gagaagacgg aagaagtgat tgccgaaaat ccaggcaaga aactggttcc
    11041 ttactatacg gtgaaagaat ttgttgaatc tttggaaacg cctcgtcgca tcctgttaat
    11101 ggtgaaagca ggtgcaggca cggatgctgc tattgattcc cttaagccat acctcgataa
    11161 aggtgacatc attattgatg gtggtaatac cttcttccag gataccatcc gtcgtaaccg
    11221 tgagctttct gccgaaggct ttaacttcat tggtaccggt gtttccggcg gtgaagaagg
    11281 agcgctgaaa ggtccttcta ttatgcctgg tgggcagaaa gaagcctatg aactggttgc
    11341 gccgatcctg accaaaatcg ccgccgttgc tgaagatggc gaaccgtgcg ttacctatat
    11401 tggtgccgat ggcgcaggcc actatgtgaa gatggttcac aacggtatcg aatacggtga
    11461 tatgcagctg attgctgaag cctattctct gcttaaaggt ggcctgaatc tttccaacga
    11521 agaactggcg cagaccttta ccgagtggaa taacggtgaa ctgagcagct acctgatcga
    11581 catcaccaaa gatatcttca ccaaaaaaga tgaagagggt aactacctgg ttgatgtgat
    11641 tctggatgaa gcggctaaca aaggtaccgg taaatggacc agccagagtg cgctggatct
    11701 cggcgaaccg ctgtcgctga ttaccgagtc tgtgtttgca cgatacatct cttctctgaa
    11761 agatcagcgc gttgccgcat ctaaagttct ctctggtccg caagctcagt cagcaggcga
    11821 caaggctgag ttcatcgaaa aagttcgccg tgcactgtat ctgggcaaaa tcgtttctta
    11881 cgcccagggc ttctctcagc tacgcgctgc gtctgaagag tacaactggg atctgaacta
    11941 cggtgaaatc gcgaagattt tccgtgctgg ctgcatcatt cgtgcgcagt tcctgcagaa
    12001 aatcaccgat gcttatgccg aaaatccgca gatcgctaac ctgctgctgg ctccgtactt
    12061 caagcaaatt gccgatgact accagcaggc gctgcgtgat gtcgtcgctt atgcggtaca
    12121 gaacggtatc ccggttccga ccttcgccgc tgcggttgcc tattatgaca gctaccgttc
    12181 tgctgttctg cctgcgaacc tgatccaggc acagcgtgac tatttcggtg cgcatactta
    12241 taagcgcatt gataaagaag gtgtgttcca taccgaatgg ctggattaat ctgatttaaa
    12301 tcaattaatc aaagcaaggc ccggagaaac cctccgggct tttttattat acaaagcggc
    12361 aggttatggc ctttttttat aatttatgat taaaaaagcg atataataca gagccgcaca
    12421 gcaggatcgc tgccttgaca gttcatctac atcagcgtta aaaatcccgc agtagatgaa
    12481 gctgtggtgg tggattaatg accactctaa atgtttaacc ggaagaagtc agagctaatg
    12541 aaaataacaa tttcaggaac aggttatgtt ggtctttcaa atggtattct gattgcgcaa
    12601 aaccacgaag tggttgcact ggatatcgtt caggccaaag tggacatgct taacaagaag
    12661 cagtcaccga ttgttgataa ggagattgaa gagtatctgg cgactaaaga gctcaatttc
    12721 cgcgctacga cagataagta tgacgcgtat aaaaatgccg attacgttat tattgccacg
    12781 cctaccgatt atgacccaaa aacaaattat tttaatacct caagcgtgga agcggtcatc
    12841 cgtgatgtga cagaaattaa tcccgacgct gtaatgatta taaaatcaac tatccctgtc
    12901 ggttttacag agtccattaa agaacggttt ggtattgaaa atgtgatctt ttcgccggag
    12961 tttttacgtg aagggaaagc actttatgat aacttacacc catcacgcat tgtgattggc
    13021 gagcaatccg atcgcgctca acgttttgca gcgttattac aggaaggcgc cattaagcaa
    13081 gatataccaa cgttgtttac tgactcaacc gaagctgagg cgattaaact ttttgctaat
    13141 acttatctgg cgatgcgtgt ggcttatttc aatgaattag atagttacgc tgaaagtctg
    13201 gggcttaatt cccgccagat tattgagggt gtatgccttg acccgcgtat cggtaatcac
    13261 tacaacaacc cgtcattcgg atatggtggt tattgtctgc cgaaagatac taagcagtta
    13321 ctggcaaact atcagtcagt accgaataac ttgatctcag caattgtcga tgccaatcgc
    13381 acgcgaaaag actttattgc cgattctatc cttgcacgta aaccgaaagt tgttggcgtc
    13441 tatcgtctga ttatgaagag tggttcggac aatttccgtg cttcatcgat tcagggtatt
    13501 atgaagcgaa tcaaggcgaa aggtgtgcca gtaatcgttt atgagccagc gatgaaagag
    13561 gacgattttt tccggtcgcg cgtggtacgt gatctggatg cgttcaaaca agaagctgat
    13621 gttattattt ctaaccgtat gtctgccgat ctggctgatg tagcagataa agtttatacg
    13681 cgcgacttgt ttggcaatga ttaattattt tgtttcattc taagaaaagg ccctaatata
    13741 ttagggcctt ttcttatggt tttgtaaaat caaactttat agaagttacg ataccattct
    13801 acaaagttct ttaccccctc tttaactgac gtttcaggtt tgaatcctat tacgtcatac
    13861 agtgcttttg tatcagcact ggtttccagt acatcaccgg gttggagagg catcatattc
    13921 ttgttggctt caatacccag agcctcttct aaggcattga tatagtccat caactccaca
    13981 ggcgaactat taccaatgtt atagacacga tatggtgctg aacttgttgc tggcgagcct
    14041 gtttctacag cccagtgtgg atctttttct ggaataacat cctgtaagcg aataatagct
    14101 tcggcaatat catcaatata agtaaagtca cgcttcattt tgccgaagtt gtaaacatca
    14161 atgcttttac cttccagcat ggctttagtg aatttaaata atgccatatc cggacgtccc
    14221 catggaccat aaaccgtaaa gaaacgcagc cctgtggtcg gtaagctata caaatgagaa
    14281 tacgtatgag ccatgagttc attcgctttt ttagttgctg cataaagcga aacaggatga
    14341 tctacagagt catctgtaga gaaaggcatc ttgcggttca tgccataaac agaactggag
    14401 gaagcgtaaa gtagatgctg aacattatta tggcgacatc cttctagtat gttcaggaat
    14461 ccaatcaggt ttgcatctgc atatgcattg ggattttcaa gggagtaacg tacaccggct
    14521 tgcgcagcga ggtttattac gcgatcgaac cgctcgtcta caaacagtgc cgccattttc
    14581 tcgcgatcgg ccaggtcaat tttataaaaa ctgaaattgt cgtgcttgag taaatcaagt
    14641 cgtgcttgtt tgaggttgac atcgtaataa tcatttaagt tgtcaatgcc tacaacctga
    14701 tgaccagctg caagaagccg tttacttaca tagaaaccga taaagccagc agctcccgta
    14761 accagaaatt tcatttataa tcctcgctca ggctagaata tagccaattt tcatctagca
    14821 taactgaaag ttaaatcata ccgttagaca agaaaaaaag ataatcggta tcagttctaa
    14881 acctggctgt tttttctggt aacgtgctca ttttacaatc aaagctgttc taagctgact
    14941 atacaagccg acgtcattat ctccaaccgt atggcagaag agcttaagga tgtggcagat
    15001 aaggtctaca cccgcgatct ctttggcagt gactaacatc ctgttatcag ggcgattttc
    15061 gccctgattc tcttatgttc cctttgtaat aattcattat ttttatcatt tatcctatag
    15121 cattcatggc gattatcgct aaactatggc gacttaaaat tatttcgtcc gttagggtaa
    15181 tgatgagagt agaaaataat aatgtttctg ggcaaaacca tgacccggaa cagattgatt
    15241 tgattgattt actggtgcag ttgtggcgcg gcaagatgac aattatcatc tctgtcattg
    15301 tcgctattgc cctggctata gggtatctgg ccgttgccaa agaaaagtgg acttccacag
    15361 ctatcgttac tcaacctgat gtggggcaaa ttgctggtta taccaacgcg atgaatgtga
    15421 tctacggatc ggctgtaccg aaagtctccg acattcaggc ttcacttatc ggacgttaca
    15481 gcactgcttt ctcagcatta gcggaaacgc tggataatca ggaagaacct gaaaaactga
    15541 ccattgagcc taccgttaaa aatcagtcat tacctttagc ggtttcttat gtgggtgaaa
    15601 cacctgaagg tgcgcagaaa cagctggcgc agtatatcca gcaagttgac gaccaggtaa
    15661 atgaagaact ggaaaaagac ctgaaagaca acatcgcgct gcgtatgaaa aacttgcagg
    15721 attctctgaa aacccaggaa gtagtcgcgc aggagcagaa agatctgcgt atccgtcaga
    15781 ttcaggaagc gttgcagtat gcgaatcagg cgcaggtgac aaagccacag gttcagcaga
    15841 ctgaagatgt gacacaagat acgttgttcc ttttggggag cgaagcgctg gagtcgatga
    15901 ttaagcatga agcgacccgt ccgttggtgt tctcaccaaa ctactatcag acacgtcaaa
    15961 acctgctgga tatcgaaagc ttaaaggttg atgatcttga tattcatgct taccgttatg
    16021 tgatgaaacc cacgttacct attcgtcgcg atagcccgaa aaaggcaatt accttgattc
    16081 tggcggtgct gctgggaggc atggttggcg cggggattgt gctggggcgt aatgctctac
    16141 gcaattacaa cgcgaaataa tctttgcagc attaaagaaa acgggcaggg tggtgacacc
    16201 ttgcccgttt tttttgccgg atgcgacaac aacatagcat ccggtcagca cgcaactcac
    16261 tgatgccgtt tacgcaggtt ctcaattacc gtcgttaaat ccagatcctg atcctgcaac
    16321 agcaccagca ggtgatacat caaatcagac gcctcgttgg tcagttcaaa gcggtcatgt
    16381 accgttgctg ccagtgcggt ttccacccct tcttcgccca ctttctgcgc aatgcgtttg
    16441 gtgccgctgg catacagttt ggcggtatag gaggtttccg ggtcggcaga tttacgttcg
    16501 gcgagcagtt gttccagttg atacaggaac agccattggt gagcggtgtc gccgaagcag
    16561 ctgctggtgc ctttgtggca ggttggtcct ataggattcg ccagtaccag tagcgtgtcg
    16621 ttgtcgcagt ccggcgcaat atttactacg ttgaggaaat tgcccgacgt ttcgcctttg
    16681 gtccacagtc gctgtttagt gcgcgagaag aaggtgactt tgccgctttc gatggttttg
    16741 tctaaggctt ccgggttcat atagcccagc attaacactt cgccggatac cgcgtgttgc
    16801 acaatcaccg gcatcagtcc gtcggttttt tcccagtcca gttcgcgacg ttgttgttct
    16861 gttaacatat cctgatctcc acgccctgtg ttgccaggta cgcttttaat tcaccaatat
    16921 tgattatttg tttgtggaat acggaagccg ccagcgcgcc gtcaacgtcg gcatcgcgga
    16981 aggcttcgag gaagtgttcc atggtgcccg cgccaccgga ggcaatcagc gggacgtggc
    17041 aaacttcacg cacttttttc agctgctcga ggtcgtaacc gttacgcacg ccgtcctgat
    17101 tcatcatgtt gaggacgatt tctccggcac cgcgtttttg cacttcctgt acccagtcga
    17161 gcgtttccca ctgagtgacg cgggtgcggc tttcatcgcc gg
//
