LOCUS       JQ751058               14733 bp    DNA     linear   BCT 02-OCT-2012
DEFINITION  Escherichia coli strain 86-381 serogroup O180 O-antigen gene
            cluster, complete sequence.
ACCESSION   JQ751058
VERSION     JQ751058.1  GI:393186183
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14733)
  AUTHORS   Wang,Q., Perepelov,A.V., Beutin,L., Senchenkova,S.N., Xu,Y.,
            Shashkov,A.S., Ding,P., Knirel,Y.A. and Feng,L.
  TITLE     Structural and genetic characterization of the Escherichia coli
            O180 O antigen and identification of a UDP-GlcNAc 6-dehydrogenase
  JOURNAL   Glycobiology 22 (10), 1321-1331 (2012)
   PUBMED   22730467
REFERENCE   2  (bases 1 to 14733)
  AUTHORS   Wang,Q., Perepelov,A.V., Beutin,L., Senchenkova,S.N., Xu,Y.,
            Shashkov,A.S., Ding,P., Knirel,Y.A. and Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2012) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, No. 23 Hongda Street, TEDA,
            Tianjin 300457, China
FEATURES             Location/Qualifiers
     source          1..14733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="86-381"
                     /host="swine"
                     /db_xref="taxon:562"
                     /note="serogroup: O180"
     misc_feature    1..14733
                     /note="O-antigen gene cluster"
     gene            107..1192
                     /gene="rmlB"
     CDS             107..1192
                     /gene="rmlB"
                     /note="RmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-D-glucose 4,6-dehydratase"
                     /protein_id="AFN02890.1"
                     /db_xref="GI:393186184"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     EVSDSERYSFEHADICDAVAMARIFSQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDDEKKNNFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            1192..2091
                     /gene="rmlD"
     CDS             1192..2091
                     /gene="rmlD"
                     /note="RmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /protein_id="AFN02891.1"
                     /db_xref="GI:393186185"
                     /translation="MNILLFGKTGQVGWELQRSLAPLGNLIALDVHSTDYCGNFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPDFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGSGDAPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAVNKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARKAGINLALNKLNAVPTSAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPEWQVGVKRMLNELFTTTAI"
     gene            2149..3027
                     /gene="rmlA"
     CDS             2149..3027
                     /gene="rmlA"
                     /note="RmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AFN02892.1"
                     /db_xref="GI:393186186"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEDFIGG
                     DDCALVLGDNIFYGHDLPKLMETAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLEPKSNFAVTGLYFYDNDIVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            3032..3571
                     /gene="rmlC"
     CDS             3032..3571
                     /gene="rmlC"
                     /note="RmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /protein_id="AFN02893.1"
                     /db_xref="GI:393186187"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSGKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYYSESDRGIIWNDPMLKIQWPLE
                     VEPILSEKDQKQAHFLTKQ"
     gene            3616..4920
                     /gene="wbpA"
     CDS             3616..4920
                     /gene="gnaA"
                     /note="GnaA"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP glucose 6-dehydrogenase##"
                     /protein_id="AFN02894.1"
                     /db_xref="GI:393186188"
                     /translation="MKNLLINKINDKDAVIGIVGLGYVGLPLMLRFSEVGYKVIGYDI
                     DQKKVDELTAGRSYIEHISASSIQDANKRGFEATTDFSRAGEADTLILCVPTPLNKYR
                     EPDLSFVLNTTDALVPHLRKGHFVSLESTTYPGTTDEELLPRIESRGLKVGEDIFLCY
                     SPEREDPGNPNFTTRTIPKVCGGVTEDCLEIGLALYGAVIDQVVPVSSTRAAEMTKLL
                     ENIHRAVNIGLVNEMKIVADKMNIDIFEVIRAAATKPFGFVPYYPGPGLGGHCIPIDP
                     FYLTWKAREYGVNTRFIELAGEVNSSMPDWVVDKVVHALNLDKKSVNGSNILVLGIAY
                     KKNVDDMRESPSVFIMEKLRSLGAEVSYSDPHVATFPKMREHHFELASVTLTKEKLSS
                     YDCVVIATDHDKFDYELIKEHSKFIVDTRGKFRDNEKNVVRA"
     gene            4922..5980
                     /gene="wbpB"
     CDS             4922..5980
                     /gene="mndA"
                     /note="MndA"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="AFN02895.1"
                     /db_xref="GI:393186189"
                     /translation="MNNYEPIVDRKIKFALVGCGRISKNHFASLEKHAERAELVDVCD
                     IDSNALERAVKSTGANGHKDINSMLEKSDADVVILTTPSGLHPVQAIKSFANGFHVVS
                     EKPMATRMEDGVAMVKAADEANKRLFVVKQNRRNATLRLLKDAIESGRFGRIYMVNVN
                     VFWTRPQDYYNASPWRGTWEYDGGAFMNQASHYVDLLEWLIGPIDSLFAFTGTLERKI
                     EAEDTGAVALKWRCGAMGSMNVTMLTYPQNLEGSITILGEKGSVRIGGVAVNEIQHWE
                     FSEPHEMDTRIKEVSYQTTSVYGFGHPLYYDNVINVLRGNAVPETDGRQGLKSLEVLS
                     AIYRSARDGKKVGLPLDL"
     gene            6007..6585
                     /gene="wbpD"
     CDS             6007..6585
                     /gene="mndC"
                     /note="MndC"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="AFN02896.1"
                     /db_xref="GI:393186190"
                     /translation="MTYTVHETAIVDQGAVIGDGTKVWHWVHICGGAQIGKNCSFGQN
                     VFVGNDVKIGNNVKVQNNVSIYDAVHLEDDVFCGPSMVFTNVYNPRSHVLRKHEYRKT
                     LVRKGATIGANATIVCGNEIGEYAFIGAGSVVNKDVKPYAMMVGVPARQIGWISRHGA
                     KLDLPLSGNATAKCSETGESYRLIHDHIEFVE"
     gene            6588..7688
                     /gene="wbpE"
     CDS             6588..7688
                     /gene="mndB"
                     /note="MndB"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="AFN02897.1"
                     /db_xref="GI:393186191"
                     /translation="MEFIDLSAQYRYLKTQIDTAISKVLQTGSYIMGSEVQELEIELA
                     RYVGVKHAITCANGTDALTLALMTLGIKEGDAVFCPTFTFFATAETIAFENATPIFVD
                     SEEDTFNISAADLEKQIEKVIAEGKLKPKAIIAVDLFGLPAEYDAIKKIADQYDLKII
                     EDAAQGFGGHFNGKKAGSFGDISTTSFFPAKPLGCYGDGGAIFTDNDEYASIIRSLRI
                     HGKGKDKYDNVNIGMNSRLDTIQAAVLLEKLKVFPQELEARNNLATQYTEALRGIYKT
                     PVVPEGYFSSWAQYTIIADDRDMVMNKCKEQGVPTMVYYTTCLHLQTAFEKLGYHEGC
                     FPVAEKLSKSVLSLPMHPYMAKDSFEKIIKAL"
     gene            7685..8944
                     /gene="wzx"
     CDS             7685..8944
                     /gene="wzx"
                     /note="Wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AFN02898.1"
                     /db_xref="GI:393186192"
                     /translation="MSLNYIRVKSEFFRNVVTLIFGTTIAQLLPILISPILTRAYTPE
                     EFGVFAMFITLGTILGTIATGRYELAIMLPKTQRKALSVAILSVVLTTLVCFIAIGFF
                     YGFRNNIAYLLDHYAIAPWLLLVPLYSFFYGNYQVVNYWHNRHIRFKTLSLSRVLQSF
                     VTCCIQLIAGIFHLGIGGLIIGLIIGQCTSFLWLFICGWMEWKTLFDKLHLRRVLFEG
                     RKYISFPLIDGPTSLLNVLSSQLPNILFAILFSPTAAGYYFLTQRILQAPVTLVSGAF
                     LDVFKQKASEEFKIYGHAKHIYKKTFVSLFFISIIPSVVAFIILPELFSLFFGKSWYE
                     AGLFAQIMMPAIVMRFVVSPLSFIIYIAGKQKWNLICMIMLCGGVIASLFLCKDATEA
                     MKTISASYVIYYCLHLVISMRLAGFFK"
     gene            9096..10310
                     /gene="wzy"
     CDS             9096..10310
                     /gene="wzy"
                     /note="Wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AFN02899.1"
                     /db_xref="GI:393186193"
                     /translation="MFNALPGGILIYYTNVTNSVLDKESAEYRIMGLYLILYMMIAFP
                     LGLLFIKPFFFKVGDIGFLTRYQNKPLVGLASTNDAHARVMLTLLTGGCALSLLYVTW
                     SVGNIPQLSFIYHMTHDDILRIRVENSRHFNGLLFIKTIFFEQLTPLLSLVVYAFYRQ
                     TKKRYDKLLFVILFIMSLYTVTFSLSKSTLVVYLAFFIFMRIYLNGRIPVMKFFIAAA
                     ILFILLIFMFFIVTDQSTADVIVYLLNRMFIDQVSGLYLMLQLFPQTYPFIGVDSLSS
                     ILSSLMGAEFVQPSTRLAMEYAFPTAVAEGKMNLLSTLFIGEAWANFGWLGVAISPLY
                     VGMITGFIYYFVLSKRKTPLLIGLLTYCSFGTNFSSQLNMYIYNSVMISLILALGLIY
                     FISVLAGQLSKR"
     gene            10381..11472
                     /gene="wbpI"
     CDS             10381..11472
                     /gene="mndD"
                     /note="MndD"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="AFN02900.1"
                     /db_xref="GI:393186194"
                     /translation="MKMRIVTILGARPQFIKAGSVSREIQRQKNSGKNIDEVIIHTGQ
                     HYDMNMSDVFFKEMKIPKPDYYLGIGGNNHGAMTGQMIEAIEGILIKEKPDAVMVYGD
                     TNSTLAGAIAASKLHINVAHVEAGLRSFNMSMPEEINRILTDRVSSLLFCPTRAAMDN
                     LEKEGIRSWGNGVNAILSGDVMQDGALFYKKFAQKPSNLDLADDFILCTIHRAENTDV
                     SERLVSIIDALTEIAMTRQVVLPLHPRTKKVILSQNIDISKLTIIEPVGYLDMVWLIS
                     NCAIVMTDSGGLQKEAFFFSKPCLTLRDETEWVELVENGFNILVGANKQSILNAYTNC
                     VNINTDYLGGDLYGNGKASKKIVNELLNI"
     gene            11525..12604
                     /gene="wfcS"
     CDS             11525..12604
                     /gene="wfcS"
                     /note="WfcS"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFN02901.1"
                     /db_xref="GI:393186195"
                     /translation="MKIAYLIRHDITRNDGVTKKILGQIDHWKNLGHEVQVYAYLPRI
                     GDSILPAKQYVMGGVIKSRIFLDKKLLMDIDSFCPNMIYFRYDTWSRSLSKLLTSYPS
                     IVELNTLDLSEFFELIKLEKNIKSILRYCAYRMLRSLVLRKVVGIVSVTNEINRDKSN
                     AQYNKPSIAIPNGIDLSTYNSIKQNARDNSRIGLFFIGTPGQPWHGVDYIEQLASKMK
                     ALDFHIVGIDGVNHENVYYHGYLDADKYKKILKKCDICIGSLGLHRINMKEACPLKVR
                     EYLAYGYPIILGYHDTAFVNEKNNPNFAYYLNTKDGISQIMIDGLQDFIIRNKDVIVT
                     SNDVAYIDTKVLEARRISFFKRVIN"
     gene            12626..13462
                     /gene="wfcT"
     CDS             12626..13462
                     /gene="wfcT"
                     /note="WfcT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFN02902.1"
                     /db_xref="GI:393186196"
                     /translation="MDKCPLIDILLPVFNGSKYLRQQLDSLLNQSYKNIRVIIRDDLS
                     SDDSRNLIKNYAENTDGKIVYIDSDKNLGSSLSFHELLKQSTADYVMFCDQDDVWLPN
                     KVHDSFTAITNANLFCNNIRCVFTDLVIVDEALNELHRSMRGALKFKAVSDDYRNYLC
                     QSQITGCTMILNRPAVELLKKFPPPNRHIIHDHWYSVIVSIYGEVRYLDKPTILYRQH
                     FNNQVGFKNINLNYFIQKIKAIFKIYHYDMGINYALPKEYQIGTMQWFIRKVRLNLER
                     IN"
     gene            13500..14555
                     /gene="wfcU"
     CDS             13500..14555
                     /gene="wfcU"
                     /note="WfcU"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AFN02903.1"
                     /db_xref="GI:393186197"
                     /translation="MKKISVIGIVGLPAAYGGFETLVENLTKNASSDIHYEVFCSSRQ
                     HSSKLKKYNGAVLRYVPLKANGIQSIFYDIFSVFRSLTSKPDVILVLGVSGAVVLPIV
                     KLFSRAKIITNIDGLEWKRDKWRKLARCFLKFSEFLAVKYSDVVITDNQAISDYVLEE
                     YGNTSSVIAYGGDHSLITSAVNLPKYSAPFYLTICRIEPENNIHIILEAFSQTNHNLK
                     LIGNWHSSIYGKELFEKYSRFSNIELIHPIYELEKLYVLRETCCGYIHGHSAGGTNPS
                     LVEAMHFSKPVYAYDCIFNRFTTENGAFYFCNSQQLIRLLNDDSLNTCKNAQLMKQIA
                     MSRYTWKTITSQYEELY"
BASE COUNT     4486 a   2451 c   3127 g   4669 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgcattaata cctctattaa tcaaactgag
       61 agccgcttat ttcacagtat gctctgaagt aatatggaat aaaaaagtga agatactcgt
      121 tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa ataatacgca
      181 ggacagtgtt gttaatgtcg ataaattaac ttacgccgga aacctggaat cactcgctga
      241 agtttctgat tctgaacgtt attcatttga gcatgcagac atctgcgatg ccgtagcaat
      301 ggctcgtatt ttttcacagc atcagccaga cgcggtgatg cacctggcgg cagagagcca
      361 cgttgaccgt tcaataactg gcccggcagc ctttattgaa accaatattg tggggactta
      421 cgtcctttta gaagcagctc gtaattactg gtctgcattg gatgatgaaa agaaaaacaa
      481 tttccgcttc catcatattt ctactgatga agtctatggt gatttacctc atcctgatga
      541 agtaaataat acagaagaat tacccttatt tactgagacg acagcttacg cgccaagcag
      601 tccttattcc gcatccaaag catccagcga tcatttagtc cgtgcgtgga aacgcaccta
      661 tggtttgccg accattgtga ctaactgctc gaataactac ggtccttatc actttccaga
      721 aaaattgatt ccgttggtca ttcttaatgc gctggaaggt aaggcattac ctatttatgg
      781 taaaggggat caaattcgcg actggttgta tgtagaggat catgctcgtg cgttatatac
      841 cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca acgaaaagaa
      901 aaatatcgat gtagtgctca cgatttgtga tttgttggat gaaattgtac cgaaagaaaa
      961 atcttatcgt gagcaaatta cttatgtcgc tgaccgcccg ggacatgatc gccgttatgc
     1021 cattgatgcc gataaaatta gccgcgaatt gggctggaaa ccgcaagaaa cgtttgagag
     1081 tgggattcgt aaaacggtgg aatggtatct ggctaataca aattgggttg agaatgtgaa
     1141 aagcggtgct tatcagtcat ggattgaaca gaactatgag gggcgtcagt aatgaatatc
     1201 cttcttttcg gcaaaacagg gcaggtgggg tgggaactgc agcgttctct ggctcccctg
     1261 ggtaatctga ttgctcttga tgttcactcc actgattatt gtggcaattt cagcaacccc
     1321 gaaggtgtgg ctgagactgt caaaaaaatt cgccctgatg ttattgttaa tgctgctgct
     1381 cacactgcag tagacaaagc ggaatcagaa ccggatttcg cacaattact taacgcgaca
     1441 agcgtcgaag cgatagcaaa ggcagctaat gaagttggag cctgggttat acactactcc
     1501 actgattacg ttttccctgg cagcggtgac gcgccatggc tggaaacgga tgcaacagca
     1561 ccgctaaatg tttacggtga aacaaaatta gccggagaaa aagcattaca agaacattgc
     1621 gcgaagcacc ttattttccg taccagttgg gtctatgcag gtaagggaaa taacttcgct
     1681 aaaacgatgt tgcgtctggc aaaagaacgc gaagagttgg ctgtgataaa cgatcaattt
     1741 ggtgcaccaa cgggagctga attgctggcc gattgcaccg ctcatgccat tcgcgtggca
     1801 gtaaacaaac cagaagttgc tggtttgtac catctggttg ctggcggtac aacaacctgg
     1861 cacgattatg ccgctctggt atttgaagag gcgcgcaaag cggggattaa ccttgctctt
     1921 aacaaactta acgccgtgcc gacatcggct tatcctactc cagcccgccg ccctcataat
     1981 tctcgtctta atacagaaaa atttcagcag aactttgctc ttgtcttgcc tgaatggcag
     2041 gtaggcgtga aacgtatgct caatgaatta tttacaacta cagcaattta aaaatttatt
     2101 gtatctcgcc aatgatattg ggcgtgataa attaaaagga atggtgaaat gaaaacgcgt
     2161 aaaggtatta ttttagctgg cggatcaggt actcgtcttt atcctgtgac tatggcagtg
     2221 agtaaacaac tcctgcctat ttatgataag ccgatgatct attacccgct ctctacactg
     2281 atgttagcgg gtattcgcga tattctgatt atcagtacgc cacaggatac accgcgtttc
     2341 caacaattgt tgggggacgg gagccagtgg gggcttaatc tacagtataa agtacaaccg
     2401 agcccggatg gcctggcgca agcgtttatc attggtgaag attttattgg tggtgatgat
     2461 tgtgcactcg tacttggcga taatatcttc tatggacatg atttgccgaa gttaatggaa
     2521 actgctgtta ataaagaaag cggtgcaacg gtatttgcct atcacgtaaa tgatccagaa
     2581 cgctatggtg tcgtggagtt tgataaaaac ggtacagcca ttagccttga agaaaaaccg
     2641 ttagagccaa aaagtaactt cgctgtaact gggctatatt tctatgataa tgacattgta
     2701 gaaatggcga aaaatcttaa gccttctgca cgtggcgagc tggaaattac agatattaac
     2761 cgtatctata tggaacaggg gcgtttatct gttgccatga tgggacgtgg ttatgcctgg
     2821 ctggacacgg ggacacatca aagcctgatt gaagcaagta acttcattgc tacaattgaa
     2881 gagcgtcagg gattaaaagt atcttgcccg gaagagattg cttatcgtaa aggatttatt
     2941 gatgcagaac aggtgaaagt gttagctgaa ccgctgaaaa aaaatgctta tggtcagtat
     3001 ctgctgaaaa tgattaaagg ttattaataa aatgaacgta attaaaacag aaattcctga
     3061 tgtgctgatt tttgaaccaa aagtttttgg cgatgagcgc ggcttctttt ttgagagctt
     3121 taaccagaaa gtatttgaag aagctgtagg gcgtaaggtt gaatttgttc aggataacca
     3181 ttcgaagtct ggtaaaggtg ttttacgcgg gctacattat caattagaac cttatgcaca
     3241 aggaaaattg gtgcgctgcg ttgttggtga agtttttgat gtggctgttg atattcgtaa
     3301 atcttcacct acttttggca aatgggttgg ggtgaattta tctgctgaga ataagcgtca
     3361 gttgtggata cctgaagggt ttgcgcatgg gtttttggtg ctgagtgaga cggcggagtt
     3421 tgtttataaa acaacaaact actattactc agaaagtgat agaggcataa tatggaatga
     3481 tccaatgtta aaaattcaat ggccattaga agttgaacct attctgtcag aaaaagatca
     3541 gaaacaagct cactttttaa caaagcaata atgtattaaa caaaatttta ccgggattag
     3601 atctccgagg tttcaatgaa aaacttgcta attaataaaa taaacgataa agacgctgta
     3661 attggtattg ttggtttggg ctatgttggc ctaccattaa tgttacgatt ctctgaggtt
     3721 ggttataaag ttattggcta tgacattgat caaaaaaagg ttgatgagct tactgctggg
     3781 agaagttata ttgaacacat ttctgcttct agtatccagg acgcaaataa gcgagggttt
     3841 gaagcaacta ctgatttttc tcgtgctgga gaagccgata ctttaattct atgtgtgcct
     3901 acaccactca ataaataccg tgaaccggat ttgtcatttg ttcttaatac aacggatgct
     3961 ctcgtacccc atctgcgcaa aggccatttc gtttctcttg aatcgacaac ctatccaggc
     4021 actacagacg aagagctgct gccacgcata gaatctcgtg gattgaaagt aggtgaggat
     4081 atattcctgt gttattcgcc ggaacgtgaa gatcctggca accctaactt cactactcgt
     4141 accattccta aagtatgtgg aggcgtcact gaagattgcc tagagatcgg tttggctctt
     4201 tatggtgcag taattgatca ggttgttcct gtatcaagca cccgggcagc agaaatgacg
     4261 aaacttttgg agaatattca ccgtgcagtg aatatcggtt tagtcaacga aatgaaaatt
     4321 gttgcggata aaatgaatat cgatatcttt gaagttatcc gcgcagcagc gactaagcca
     4381 tttggttttg ttccttatta cccgggccca ggtcttgggg gacactgcat cccgattgat
     4441 ccgttctatt tgacttggaa agcacgtgaa tatggcgtaa atacacgatt tatcgagtta
     4501 gcaggggaag taaattcctc tatgcctgat tgggttgtcg acaaagtggt tcatgcgctt
     4561 aacttagata aaaaatctgt taatggcagc aacattctcg tattaggtat cgcatataag
     4621 aaaaatgtag atgacatgcg tgaaagtcca tctgtgttta ttatggagaa attacgctcg
     4681 ttgggcgctg aagtgtctta cagtgatccc catgtcgcaa catttccgaa aatgcgtgag
     4741 catcattttg agctcgctag cgttactctt accaaagaaa aactctcatc ctatgattgc
     4801 gttgttatag caacagacca tgacaaattt gactatgaac ttattaaaga gcattcgaag
     4861 tttatagtgg atacgcgcgg gaagtttaga gataacgaaa aaaatgttgt aagggcttaa
     4921 tatgaataat tatgaaccaa ttgttgatcg taagataaaa tttgcgttgg taggttgtgg
     4981 cagaatttct aaaaatcatt ttgcatcatt agaaaagcat gctgaacgcg ctgaattagt
     5041 cgatgtttgt gatattgact ctaacgcact tgaaagagca gttaaatcaa ctggcgcaaa
     5101 tggccataaa gatattaact caatgctgga aaaatcagat gcggatgttg ttattctgac
     5161 tactccctct ggactacatc ctgtccaagc aattaaaagt tttgccaacg gctttcatgt
     5221 tgtttccgaa aaaccaatgg cgacacgtat ggaagatggg gttgccatgg ttaaggccgc
     5281 agatgaagcg aataaacgtt tatttgttgt caaacagaat agacgtaatg ctactcttcg
     5341 tctgttaaaa gatgcaattg aaagtggccg ctttggacgt atttatatgg tcaatgtaaa
     5401 tgtattttgg acgcgcccgc aggattatta taatgcttcc ccttggcgtg ggacttggga
     5461 atatgatggg ggcgctttta tgaatcaagc tagtcactac gttgacctcc tggaatggtt
     5521 aataggtcca atcgatagct tgtttgcctt tacggggaca cttgaacgga aaattgaggc
     5581 tgaggataca ggagctgttg ccctaaaatg gcgctgtggt gctatgggga gcatgaatgt
     5641 cacgatgctt acctacccgc agaatctaga aggatcaatt actatcttag gagaaaaagg
     5701 cagcgttcgt atcggtggtg ttgcggtgaa tgaaattcaa cactgggagt tttctgaacc
     5761 acatgaaatg gacactagaa tcaaagaagt aagttatcaa acaacaagcg tctatggatt
     5821 cggtcatccc ctatattatg ataatgttat taatgtgtta cgtgggaatg ctgttcctga
     5881 gacagatgga cgccaaggat taaaatctct cgaagttctg tctgcaattt atcgttcagc
     5941 acgtgatggt aaaaaagttg gtttaccatt agatctttaa agcaatcaca aaatgtcagg
     6001 atacgtatga cttatactgt tcacgaaact gcaattgttg atcaaggagc cgtcattggt
     6061 gacggcacca aggtatggca ctgggtccat atctgtggtg gagcacaaat tggtaaaaat
     6121 tgttcctttg gacaaaatgt ttttgtcgga aatgacgtga aaattggcaa caatgtgaag
     6181 gttcaaaata atgtctcaat ttatgatgca gtgcaccttg aggatgacgt tttctgtgga
     6241 ccaagtatgg tttttacgaa tgtctataat cctcgatccc atgtattacg aaaacatgaa
     6301 tacaggaaaa cgttggtgag aaaaggcgca acgattgggg cgaatgcaac tatagtatgt
     6361 ggtaacgaga tcggtgaata tgcctttatt ggtgctggta gtgtagtaaa taaggatgtt
     6421 aagccctatg cgatgatggt tggagttcct gcccgtcaaa ttggttggat aagtcgccat
     6481 ggtgcgaaac tagatttacc gctttctgga aacgctacag ctaaatgctc tgagacaggt
     6541 gaaagttacc gactaattca cgatcatatt gaatttgtgg agtgattatg gaatttatag
     6601 atttaagcgc gcagtacaga tatctaaaaa cacaaatcga tactgccatt tctaaagtgt
     6661 tgcaaaccgg tagctacatt atgggtagtg aagttcagga acttgagatt gagcttgccc
     6721 gatatgttgg agttaaacat gcgattactt gcgcaaatgg tacagatgcc ttgacgctgg
     6781 cgttaatgac cttaggtatt aaggagggcg atgctgtttt ctgcccaacc tttacattct
     6841 ttgcaacagc ggaaactata gcttttgaaa atgcaacacc tattttcgtt gattcagaag
     6901 aagacacttt caatatttct gctgcagacc ttgaaaaaca aattgaaaag gtcattgctg
     6961 aaggtaagtt aaaacctaaa gcaattattg ctgtggatct atttggactg cctgctgagt
     7021 atgatgctat caaaaaaatt gctgaccaat acgatctaaa aattatcgaa gatgctgctc
     7081 aaggttttgg tggtcatttt aatgggaaaa aagcgggtag tttcggtgac attagtacga
     7141 ccagtttttt ccctgctaaa ccactggggt gttatgggga cggtggggcg atctttaccg
     7201 acaatgacga atatgcttca ataatcaggt cactgcgtat acatggaaaa gggaaagata
     7261 agtacgacaa tgttaacatc ggtatgaaca gccgtctgga tacgattcag gctgcggtac
     7321 ttcttgaaaa actgaaagta ttcccgcaag aacttgaggc tcgtaataat cttgctaccc
     7381 aatatactga agctttgcga ggaatataca aaactccagt tgttcctgaa ggatatttta
     7441 gctcttgggc acaatataca atcattgctg acgatcgtga tatggtcatg aacaaatgta
     7501 aagagcaggg ggtgccgacg atggtgtatt atacaacctg tcttcattta caaacagctt
     7561 ttgaaaaact tggttatcat gaaggatgtt tcccagttgc tgaaaaactc agtaaatcag
     7621 ttttaagtct tccaatgcat ccttatatgg cgaaggattc atttgagaaa ataatcaaag
     7681 cactatgagc ttaaattata ttagagttaa atcagaattt tttcgaaatg tagtaacatt
     7741 aatttttgga actaccattg cacaactact tccaatactg atcagcccta ttttaactcg
     7801 agcatatacc ccagaggaat ttggggtatt cgctatgttt ataacgttgg gaactattct
     7861 aggcacgata gctacaggtc ggtacgaatt ggcaataatg cttccaaaaa cacaacgtaa
     7921 ggcgctctca gttgcaattc tatctgtagt gctaacaaca ttggtctgtt ttattgctat
     7981 tggttttttt tatggtttca ggaacaatat tgcatattta ttggatcatt atgctattgc
     8041 gccgtggctc ttgttagttc ccttatattc atttttttat ggtaactatc aagttgtaaa
     8101 ttattggcat aataggcata taaggtttaa gacgttatct ctcagtcgtg tgttacaaag
     8161 ttttgtaaca tgctgtatac aattaattgc aggaatattt catctgggca ttggtggact
     8221 cattattggt ctaatcatag gtcagtgtac atcattctta tggttgttta tatgtggctg
     8281 gatggagtgg aaaactttat ttgataaact acatctacgt cgagttttat ttgaagggag
     8341 aaaatatata agttttcctt tgattgatgg acctaccagt ttattgaatg tattgtcttc
     8401 acaacttcca aatatattat ttgctatatt gttttcccct acagctgctg gctattattt
     8461 tctaactcag cggatattac aagcaccggt tactctggtc tctggtgctt ttcttgatgt
     8521 ttttaaacag aaggcaagtg aagagtttaa aatttatgga catgctaaac atatatataa
     8581 aaagaccttt gtgagtcttt tttttatatc tattatacct tccgttgttg catttatcat
     8641 actgcctgaa cttttctctc tattttttgg aaaatcatgg tacgaggctg gcctctttgc
     8701 acaaataatg atgccagcaa tagttatgag attcgtagtg agcccgctga gttttattat
     8761 ttatattgcg ggaaaacaaa aatggaattt aatttgcatg attatgctct gtggtggagt
     8821 aattgcttca ttgttccttt gtaaggatgc tacagaagca atgaaaacta tctcagcaag
     8881 ttatgttatt tattattgcc tgcatctcgt tatatcaatg aggttggcag gttttttcaa
     8941 gtaactgtta agcaattgac tcagcgaata tttttatatg cttattctca tagtttttgt
     9001 tgtgattata tcttggtttc tttttaaaat agctgctggc agcatgtcta ttggccaatt
     9061 aaataataat atcagcaatt ttttattata atataatgtt taatgcattg cctggtggta
     9121 tattgattta ttacaccaat gttacaaatt cggtattaga taaagagtct gctgaatata
     9181 gaattatggg actttattta atattgtata tgatgatagc attccccttg ggattgttgt
     9241 ttataaaacc attttttttt aaagttggtg atattggctt cttaacccgt tatcagaata
     9301 aaccacttgt cggactggca tcaacgaatg atgcacatgc acgagttatg ttaacattat
     9361 taacgggagg ttgtgcctta tcattattat atgtcacatg gtcagttgga aatatacctc
     9421 agctatcttt catttatcat atgacgcatg atgatatttt aagaatacgg gttgaaaact
     9481 cgcgacattt taatggcttg ttatttatta aaacaatttt ctttgagcaa ttaacgcctt
     9541 tgttatcttt ggttgtatac gctttttata gacaaacaaa aaaaagatat gacaaactcc
     9601 tatttgtcat attatttatt atgtcgctct acactgtaac attttctctt tcaaaatcaa
     9661 cacttgtagt ttatctggca ttcttcattt ttatgcgaat ctatttaaat ggtcgaatcc
     9721 ctgttatgaa gttttttatc gctgcagcta tattatttat tcttctcata tttatgtttt
     9781 tcatagttac agaccagtca actgctgatg ttattgtata tttgctaaat cgaatgttta
     9841 ttgatcaggt ttctggactg tatttaatgt tacaactatt tccgcaaact tatcccttta
     9901 ttggtgttga ttcattatca agtatcttgt caagtcttat gggggcagaa tttgtacagc
     9961 cttcgacacg cctggcaatg gaatatgcat tcccaactgc agttgcagaa gggaagatga
    10021 atcttttaag taccttgttt attggtgaag catgggcaaa ctttgggtgg ttgggtgttg
    10081 caatatctcc gttatacgtc gggatgataa ctggatttat ttattatttt gttcttagca
    10141 aacggaagac accattattg attggacttt taacatattg tagctttggg actaactttt
    10201 catctcagct taatatgtat atttataatt ctgtgatgat ttcattaata ttagctctcg
    10261 gcttaattta ctttatttct gttttggcag gacagttatc aaaacgataa gttctaatag
    10321 atatataatc gtcacttggc aaatgatatt attaataagt taataaatta aatattggag
    10381 atgaaaatga gaattgttac tattttaggc gcaagacctc aatttatcaa agcaggttct
    10441 gttagtcgtg aaattcagcg tcaaaagaac tcaggtaaaa atattgatga agtgattatc
    10501 catactggac aacattatga tatgaatatg agtgatgttt tctttaaaga aatgaaaatt
    10561 ccaaaaccag attattatct aggtattggg gggaataatc atggtgctat gacagggcaa
    10621 atgattgaag caattgaagg gatcttaatt aaagaaaagc ctgatgcagt tatggtttat
    10681 ggagatacta attcaacttt agctggagcc attgctgcat caaaactcca tattaatgtt
    10741 gctcatgttg aggctggttt acgtagtttt aatatgagca tgcctgagga aattaatcga
    10801 atactaacag atcgcgttag ttcgttactc ttttgtccaa caagagctgc tatggataat
    10861 ctcgaaaaag aaggtatcag aagttggggt aacggcgtta atgcgatttt aagtggtgat
    10921 gtcatgcaag atggcgcctt gttttacaaa aaatttgcac agaaaccaag caatttagac
    10981 ttagctgatg attttatcct gtgcacaatc catcgagcgg aaaatactga tgtgtcagag
    11041 cgtttagtat caattattga tgcattaaca gagatcgcaa tgactaggca agttgtgtta
    11101 cctttacatc cgcgaacgaa gaaagtcatc ttaagccaaa acattgatat ttccaaactg
    11161 actattattg agcctgtagg atatctcgat atggtctggt tgataagcaa ttgtgctatt
    11221 gtaatgactg atagcggtgg acttcagaag gaagcttttt tcttttctaa accctgtctc
    11281 acgttacgtg atgaaactga atgggtagaa cttgttgaaa atggttttaa tattcttgtt
    11341 ggagctaata agcagtcaat tcttaatgca tatacaaact gtgtaaatat taatactgat
    11401 taccttgggg gtgatttgta tggtaatggc aaagctagta agaaaatagt taatgagctg
    11461 ctgaatattt agaagcttct atgctaatct gtactcgata ttttatcgtt tgaattatac
    11521 aaatatgaaa attgcatact taattagaca cgatattact cgtaacgacg gagtaacgaa
    11581 aaaaatccta gggcaaattg atcattggaa aaatcttgga catgaagttc aagtttatgc
    11641 gtatcttcct cgaataggtg attcaatttt accagcaaaa caatacgtta tgggaggtgt
    11701 cataaaaagt agaatctttc tggataaaaa attattgatg gatattgata gtttttgtcc
    11761 aaatatgatc tattttcgtt atgatacgtg gagtaggagt ctttctaaac tattaacaag
    11821 ttatccttca atagttgaac ttaatacttt ggatttgtct gagttttttg aattaataaa
    11881 acttgaaaaa aatatcaaat caattttaag atattgtgca tatcgcatgc tacgtagttt
    11941 ggtactgcgg aaagttgtgg gtatagtctc ggtaacaaat gaaataaata gagacaaaag
    12001 taatgcacaa tataataagc cttcgatagc tatacctaac gggattgatt taagtacata
    12061 taatagcata aaacaaaacg cacgagataa ttctcggatt ggcttgtttt ttattggaac
    12121 tccgggacag ccttggcatg gagttgatta tattgaacaa cttgctagta aaatgaaagc
    12181 attagatttt catattgtcg ggattgatgg tgtcaatcat gaaaatgtat attatcatgg
    12241 ttatcttgat gctgataaat ataaaaaaat attaaaaaaa tgtgatatat gcatcggaag
    12301 tttaggcttg catcgtatca acatgaaaga ggcatgccca ttgaaagtcc gagaatatct
    12361 agcatatgga tatccgataa ttcttggtta tcatgatact gctttcgtca atgaaaaaaa
    12421 taatcctaat tttgcatatt atttaaatac aaaagatggg atctcacaga taatgattga
    12481 cggacttcag gattttatca ttaggaacaa ggatgtcatt gttacatcaa acgacgttgc
    12541 atatattgat accaaagttc ttgaagcgag aagaatctct tttttcaaaa gagttattaa
    12601 ttagacacgc tgggaattaa aatttatgga taagtgtcct ttgattgata ttttactgcc
    12661 tgtatttaat ggcagtaaat atctaaggca acagttggat tcactgctaa atcaatcata
    12721 taaaaatata cgtgtaataa tcagagatga tctttcttct gatgattcac gtaatcttat
    12781 aaagaactat gcggaaaata cagatggtaa aattgtctat attgattcag ataaaaacct
    12841 tggttcctca ctgagttttc atgagctcct gaagcagtct acagcagatt atgtcatgtt
    12901 ttgcgatcaa gatgatgttt ggctacctaa taaagttcat gattctttca ctgcaattac
    12961 aaatgctaat cttttttgca acaatattcg ctgtgttttc actgatcttg taattgttga
    13021 tgaagcatta aatgaattgc atagatctat gcgtggtgct cttaaattta aggcagtctc
    13081 tgacgattac agaaactatt tatgtcagag tcagattaca ggatgcacta tgattttgaa
    13141 tcgccctgct gttgagttac ttaaaaaatt ccccccacca aatcgtcata ttatacatga
    13201 tcattggtat tcggtaattg tctctattta cggagaagtt agatatcttg ataagccaac
    13261 aattctatac cgacagcatt ttaataatca agttggtttt aaaaatataa atttaaatta
    13321 ttttatacaa aagataaagg caatatttaa aatatatcac tatgacatgg ggattaatta
    13381 tgctttacca aaagaatacc agattggcac tatgcaatgg tttataagga aagtcagatt
    13441 gaatttagaa cgtatcaatt gaatacatgt gattataata aataaacgga ttggttagaa
    13501 tgaaaaaaat ttccgtgata ggaattgttg gattacctgc ggcatatggt gggtttgaaa
    13561 ctttagttga aaatttgacc aaaaacgcat ctagtgacat tcattacgag gttttttgtt
    13621 catcaagaca acattcttca aaacttaaaa agtataatgg tgctgtattg cgttatgttc
    13681 cgttgaaggc aaatggcatt caaagtatat tctatgatat tttttcagtt ttccgatccc
    13741 ttacgagtaa acctgatgtt atattagtgc ttggtgtttc gggggcagtt gttttaccca
    13801 ttgtgaaact tttctctcgt gcaaaaatta ttacaaatat tgatgggttg gaatggaagc
    13861 gggacaaatg gagaaaatta gcaagatgtt ttctcaagtt ctcagaattt ctggctgtga
    13921 aatattcaga tgtggttatt accgataacc aagctatatc tgattacgtg ttggaagaat
    13981 atggaaatac tagctctgtg attgcttacg gtggtgatca ttcgttaata acttctgctg
    14041 ttaatctgcc gaaatatagt gctccatttt atttgaccat ttgtagaatt gaacctgaga
    14101 ataatataca tattatactg gaagcttttt ctcagacaaa ccataactta aagttgatag
    14161 gtaattggca ctcaagtata tacggtaaag aattatttga aaaatactcg cgattttcaa
    14221 atatagaatt gattcatcca atttacgagc ttgagaaatt atatgtttta cgggagacat
    14281 gctgtggtta tatacatgga cactctgctg ggggaactaa cccttcactt gttgaggcaa
    14341 tgcatttttc caaaccagtt tatgcttatg attgtatctt taatcgattt acaacagaaa
    14401 atggtgcatt ttatttctgt aatagccaac aacttatccg actgctaaat gatgattcgt
    14461 tgaatacatg taaaaatgca cagttaatga agcaaatagc gatgagcagg tacacatgga
    14521 aaacgattac aagtcaatat gaggaactgt attagcttta gtattatgag gtttttattt
    14581 gaaataaact tacacttcaa gaatcacact ttcgttatca gtgattgacc tatttaggtt
    14641 aacattttca gaaatagcag ccgcacacgc ggtaacccct gacaggagta aacaatgtcc
    14701 aagcaacaga tcggcgtcgt cggtatggca gtg
//
