LOCUS       AB812079               16810 bp    DNA     linear   BCT 13-DEC-2014
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: 99-1287.
ACCESSION   AB812079
VERSION     AB812079.1  GI:733167498
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. (2014) In press
   PUBMED   25428893
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16810)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..16810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="99-1287"
                     /db_xref="taxon:562"
                     /note="serogroup:O182"
     gene            <1..905
                     /gene="wcaM"
     CDS             <1..905
                     /gene="wcaM"
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ02007.1"
                     /db_xref="GI:733167499"
                     /translation="YAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILIS
                     DHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVEN
                     GKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIK
                     GKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKP
                     QHLFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSV
                     DIDRINHHIVNVEKINFRLPERRE"
     gene            1080..1973
                     /gene="galF"
     CDS             1080..1973
                     /gene="galF"
                     /note="O182_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ02008.1"
                     /db_xref="GI:733167500"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     CDS             2284..3114
                     /note="O182_02"
                     /gene="wbxK"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="BAQ02009.1"
                     /db_xref="GI:733167501"
                     /translation="MYLRKVDMININMRKNNASREYLDICWDTFFKSKNRGLDLVAHF
                     PWLTSGSSSFISYEVEKNNQIVGGLIVLERDIRNLKIGLVGLVCVKKECRGQGIFKAL
                     MRKMLISCKESHFSALYLWSSKHSIYEKFDFELHDKNILVKIKVINDGDFGKPILNNY
                     GDVNIIHRTDLAVPPFASGIYELKTECGSIVYCESNGNKYIINHENTVENVILEFGKF
                     NKGEFFLNTIIDRKNNILSMKDYFDVEIIRQNIQMVNLIDKASIDKVYKINFLVNERI
                     "
     CDS             3117..4214
                     /note="O182_03"
                     /gene="wbxL"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminotransferase"
                     /protein_id="BAQ02010.1"
                     /db_xref="GI:733167502"
                     /translation="MIPIVKVFMPPKDVLMPALEQVLYSGMVGEGKHVYTFEDKFKKL
                     FGINNAIAVSSGTAALHMALILAGIKKGDEVITTSMTAEPTNTSILQAGGVPVFADVD
                     KNTGCLDPDSIIEMISDKTKAILLVHYAGYLVDVPELKEKLRRKINRKIKIIEDCAHA
                     LGATIDGRMVGTFGDYGIFSFQAIKHMTTIDGGFLVIKDTSQTKLAKKMRWFGMEKGV
                     ERTSLDLDVLGFKYNMNNVTGVIGEIQLDYVKDNISRHQKNAQFYNMMLNDITGIEVV
                     ESRQSHSPSYWLYTLLCDDSDDIINKLNEINVAASKLHRPNHYHSLLSQCRRASMENL
                     DSFYKRLVHLPCGWWIDEITRSKIIEALRRG"
     CDS             4216..5367
                     /note="O182_04"
                     /gene="wbxM"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminotransferase"
                     /protein_id="BAQ02011.1"
                     /db_xref="GI:733167503"
                     /translation="MINLFNVSFDHDIQKVVEDTLVSKAIASGAHVCQLESRLSDLFS
                     EKYALTVNDMTNGITLLLDVFGLKKGDEVLVSPFSCLATTSPMALLGIKPVWVDIEPS
                     TLAMNIKSIESNITKKTKAILVYHVAGYPSDIVEISKIAKKNNLLLIEDCNSAFGSKY
                     RNKSLGSWGDATVLSFYPNRQVGSIDGGCVLFKDKNHYELAKLRRRYGVDFNDFRKVN
                     GEINEAKDVETYGYSATINNVSAGIIMAKLPKYANEMLLTQKNVSKLNNVVMSLQNKY
                     KGSIKLVTPLKDSCVNNWVYFVRVRNKESFMNEMKKKGVHVSSLHLRNDLYSCFHAER
                     KHLTGVDIISDTLVAIPCGYWISEEDIDIIGKYIEQTLIFLVKNETSYN"
     gene            5375..6718
                     /gene="wzx"
     CDS             5375..6718
                     /gene="wzx"
                     /note="O182_05"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ02012.1"
                     /db_xref="GI:733167504"
                     /translation="MLYFGKFGALAVGFFLMPAYSYYLGPASFSTVAFILSLVNAAVT
                     LDFGMSTIIGRDSSDSTISNDKKYKQFLASQFLVIFFYLSVFSIVAIYSFLFQKGDLG
                     VKISFLSVILIMSSLIQNISVSYLNGIKEFKISGSLIFLSVTLRGVISLLVIKYIDNN
                     IISFMSAQSIIGLLFSLIFMLLPSRLDKEIKINNSWGGFFEPSIYIKFLLPLVKKGFP
                     LLFMGVSATLVMQFDKIILSYYASDATLSAYYLAFTFSTIPILAIAGPIKQYFQPHIV
                     SYLTQNNVLYRKNSIVFFWCLIVFVAVPSALGYSSLYQILSIWLGNNELIDKVDFFSH
                     ILLPAFVLGAISYFPSVLLVVAEDYRFQSVFAIITSIIFVFLLFILAISSNVRFIPWL
                     FIGYFCVVILFCSIRCFSLTKVNLCIFDMVKNSPFAILIIALSYWVGNIVSAMIF"
     gene            6772..7782
                     /gene="fnlA"
     CDS             6772..7782
                     /gene="fnlA"
                     /note="O182_06"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase"
                     /protein_id="BAQ02013.1"
                     /db_xref="GI:733167505"
                     /translation="MFKDKTLMITGGTGSFGNTVLKRFLNTDVKRIIVFSRDEKKQED
                     MRIALANPKLKFIIGDTRDYESVYRAMNGVDYVFHAAALKQVPSCEFYPMEAVKTNVN
                     GTENVLNAAIDCGVKRVVLLSTDKAVYPINAMGISKAMAEKVLIAKARRQDNDKTIMC
                     ATRYGNVMASRGSVIPLFFEKIKLNKPLTVTDPNMTRFLMSLEDSVDLVLHAFKNGRQ
                     GDIFVQKAPACTIGDLTEAIKQLCDSNIETKIIGTRHGEKLYESLVSREEMVKAEDMG
                     RYYRIPADNRDLNYDKYIIKGQIDINKLDDYTSHNTERLNVEQIKALLLTLKYVQKEL
                     GI"
     CDS             7834..8997
                     /note="O182_07"
                     /gene="wbaA"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ02014.1"
                     /db_xref="GI:733167506"
                     /translation="MVYMIKLSILIPTKNRSEYLDDVLSSLLTWKRDDFEVIIQDNSD
                     DLKTHNVVEKYRYDHRVKYFYVKEHLSVIDNFEYAVKNSSGKIITAIGDDDGILPIVM
                     DVADWMLKNSVEAVLTNKAEYVWPDLISKYNSNYNNSKLKFNKNYSYDYXKINCIDEF
                     LQVCRHGGTSMGRLPRIYYGLVCRESLDHVFNLTGQYFPGPSPDMANAASLSLVIKSF
                     YYLNFPVFIAGSSSKSTAGLGLKRKHLGAISEIKHLPMNTMAEWNKNIPDFWSGPTIW
                     AQSISSVIKKMEPTYDLNYEYLYARCFVFFPQKSKCIYLALRQYGISGKTVIKIIYWY
                     VYNWKLRFVHLLPKLLAKIGKKDHNIITIDEQLKISQAVDSLLSFTQKSDLSK"
     gene            8994..10220
                     /gene="wzy"
     CDS             8994..10220
                     /gene="wzy"
                     /note="O182_08"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ02015.1"
                     /db_xref="GI:733167507"
                     /translation="MSENILSRLTLNSAQKCLLTGLIIILLGPYGGALGFALVVCGAG
                     LEKGRIERYILCVFSFFIFTLCSVQGVFHSSNFNTISLFTALCTVMVLFLVDNNSLIL
                     ILDKLIFVISVTVPIALIIAISQTLGVYPFEFRSNEVLGVMRFTFLFAEPSHYSILLS
                     LCSLISLVKKTNLFTRFILLFGLLMTWSLSGFCIFTFGYIAYKFYTRGIIKYFIISLL
                     LGSIITFLWFFKLQYSDFWLVAKIDSIIQLFSGNTVGASSALLRFNSIIIGPYFINEK
                     INSDNFLLSFFGLGFGNLSDFVSVYYQSNFGLPDVYDANNIISNVIISSGIVGLVFYI
                     LSFSYFTGLNLVNILFAAIILLCLSLFSGYAFGPFAILTLLLSRAFILLVINHDKARI
                     NRCTYFNTWFENKTGS"
     CDS             10153..10938
                     /note="O182_09"
                     /gene="wbsG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ02016.1"
                     /db_xref="GI:733167508"
                     /translation="MTKHASIDVLISTHGSRIKQVPNILHDFKRNVRYIICHQNFELY
                     EPNEILLNRTDVIYIRSTSNGVTKSRNILLDKADADLIYFCDDDVVLSENFDEIIINS
                     HKKYSDDVILFNIRDENGELRKKYPNEVVKKTRFNILSVGTIEISLKNSPLLPKFPED
                     MGAGTSLPVGDEAVFLSTFIKKGKSIRYIPNTIAIHPRESTGLEVTNGSIYSRGVALN
                     RIYGIYAFLVAIFFLLRRRSLLKTDRGVLQGGILFFKGVLLGK"
     CDS             10951..11700
                     /note="O182_10"
                     /gene="wbdV"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ02017.1"
                     /db_xref="GI:733167509"
                     /translation="MKISIVTATYNSWPYIKETYDSIANQSYKNWEWHVTDDCSSDYT
                     YQYLKQLADRDCRIVIHRNEINSGAAVARNKSLSQCSGDYIAFIDSDDLWNPEKLESQ
                     LKFMVDNQLDFSFTGYEVIDESGKSIKKYIDINQTQAISYEDMLKKKATLGCSTVMLR
                     RLAFSELSMPDLRTGQDYALWLKLLKTGIRAVPYNKSLMKYRIVSSSISRNKYRKAKR
                     QWQIYRELEKLSLVKSIYCFLFYAWRAIFRK"
     gene            11835..13241
                     /gene="gnd"
     CDS             11835..13241
                     /gene="gnd"
                     /note="O182_11"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ02018.1"
                     /db_xref="GI:733167510"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            13490..14656
                     /gene="ugd"
     CDS             13490..14656
                     /gene="ugd"
                     /note="O182_12"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ02019.1"
                     /db_xref="GI:733167511"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAECFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERELATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            14695..15780
                     /gene="wzz"
     CDS             14695..15780
                     /gene="wzz"
                     /note="O182_13"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ02020.1"
                     /db_xref="GI:733167512"
                     /translation="MLLTEQFIIFITYPIAFTRIIAKLWRLKIISSVRVMMRVENNNV
                     SGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPD
                     VGQIAGYTNAMNVIYGPAVPKVSDIQASLIGRYSTAFSALAETLDNQEEAEKLTIEPT
                     VKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSL
                     KTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSDALESMI
                     KHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITL
                     ILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(15878..16489)
                     /gene="hisI"
     CDS             complement(15878..16489)
                     /gene="hisI"
                     /note="O182_14"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ02021.1"
                     /db_xref="GI:733167513"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     5074 a   2942 c   3578 g   5215 t
ORIGIN
        1 attacgccat cttacgccag ggatttcata accagattat cggtgccaac atcaccaact
       61 gtaagttcag tgatttacag ggcgacgcca ttgaatggaa cgtggcaatt aacgacagtg
      121 atattttgat ctctgaccat gtcatcgagc gcatcaactg taccaacggc aaaatcaact
      181 ggggaatcgg cataggcctt gcggggagta cttatgataa taactacccg gaagaccagg
      241 cagtgaaaaa ctttgtcgtg gcgaatatca cgggatcgga ttgtcggcag ttgatccatg
      301 ttgaaaatgg taaacatttt gttattcgta atatcaaagc ccgcaatatc acgccggatt
      361 tcagtaagaa agcaggtatt gataacgcca cagtcgctat ttacggttgt gacaatttcg
      421 tgattgataa tattgaaatg attaatagcg ccgggatgtt aatcggctat ggggtaatta
      481 aaggcaaata tctctcgata ccacaaaatt tccgagtgaa taatattcag ctggataata
      541 cccaccttgc ttataaattg cgcggcatcc aaatctccgc cgggaatgct gtctcctttg
      601 tggcactgac taacattgag atgaagcgtg cgtcgcttga gctacacaat aaaccgcaac
      661 acctttttat gcgaaatatc aatgtgatgc aggaatcctc agttggacca gcattgagca
      721 tgaacttcga catgcgcaaa gacgttcgcg gcgtctttat ggcgaaaaaa gaaacactgc
      781 tgtctcttgc aaatgttcat gcggtgaatg aaagagggca aagctccgtc gatatcgaca
      841 ggattaatca ccatattgtt aatgtggaaa agattaactt tagattgccg gaacggaggg
      901 agtagatttg cgaccattcc tggaaaaatg gagccatact taggaacaat gctactgcaa
      961 tccacaacga agcggcgtaa catcacaagt aattcagtaa tcaattcagg gtaattgatg
     1021 ctggcgaaaa aaatcgaaca agctataatt cagcaaccat tttacaggtg gatgaaataa
     1081 tgacgaattt aaaagcagtt attcctgtag cgggtcttgg gatgcatatg ttgcctgcca
     1141 ctaaggcgat tcccaaagag atgctaccga tcgtcgacaa gccaatgatt cagtatattg
     1201 ttgacgagat tgtggctgca gggatcaaag aaatcctcct ggtaactcat gcgtccaaga
     1261 acgcggtcga aaaccacttc gacacctctt atgaattaga atctcttctt gagcagcgcg
     1321 tgaagcgtca gctgctggcg gaagttcagt ccatctgccc gccgggcgtg accattatga
     1381 acgtacgtca gggcgaacct ttaggtttag gtcactccat tttgtgtgca cgacccgcca
     1441 ttggtgacaa cccatttgtc gtggtgctgc cagacgttgt gatcgacgac gccagtgccg
     1501 atccgctgcg ttacaacctt gctgccatga ttgcgcgttt caacgaaacg ggccgcagcc
     1561 aggtgctggc aaaacgtatg ccgggtgacc tctctgaata ctccgtcatt cagaccaaag
     1621 aaccgctgga tcgcgaaggc aaagtcagcc gcattgttga atttatcgaa aaaccggatc
     1681 agccgcagac gctggactca gacatcatgg ccgttggtcg ctatgtgctt tctgcagata
     1741 tttggccgga acttgaacgc actcagccag gtgcatgggg acgtattcag ctgactgatg
     1801 ccattgccga actggcgaaa aaacagtctg ttgatgccat gctgatgaca ggtgacagct
     1861 atgactgcgg taaaaaaatg ggttatatgc aggcatttgt gaagtatgga ctacgcaacc
     1921 tgaaagaagg ggcgaagttc cgtaaaggga ttgagaagct gttaagcgaa taatgaaaat
     1981 ctgaccggat gtaacggttg ataaggaaat tataacggca gtgaagattc gtggcgaaag
     2041 taatttgttg cgaatattcc tgccgttgtt ttatataaac aatcagaata acaacgagtt
     2101 agcaatagga ttttagtcaa agttttccag gattttcctt gtttccagag cggattggta
     2161 agacaattag cgtttgaatt tttcgagtta agcgcgagtg ggtaacgctc gtcacatcgt
     2221 agacatgcat gcagtgctct ggtagctgta aagccagggg cggtagcgtg ttttatttac
     2281 tgcatgtatt tgagaaaagt agacatgata aatattaata tgcgtaaaaa taacgcaagc
     2341 cgagagtatt tagatatttg ttgggatact ttcttcaaaa gtaaaaatag aggcctcgat
     2401 cttgtcgctc atttcccgtg gctaacttca gggtcgtcta gttttatttc ctatgaagtc
     2461 gaaaaaaata atcaaatagt tggtggtctc attgttctag aaagggatat aaggaactta
     2521 aaaattggac ttgtgggttt agtttgcgtc aaaaaagaat gccggggaca aggtattttt
     2581 aaagccctta tgcgtaaaat gctgatatcg tgcaaagagt ctcatttttc ggcattatat
     2641 ctttggtcgt caaagcatag tatttacgaa aagtttgatt ttgaacttca tgataaaaat
     2701 atcttagtca aaatcaaggt gataaatgat ggtgattttg gaaaacccat attaaataat
     2761 tatggagatg taaatatcat acaccgcaca gatttggctg ttccaccttt tgcatctggt
     2821 atttatgaat taaaaactga atgtggctct attgtatatt gtgaaagcaa tggtaataaa
     2881 tatataatta accacgaaaa tacagtcgag aatgttatac ttgaatttgg aaaattcaat
     2941 aaaggtgagt tttttttaaa taccataatc gatcgaaaaa ataatatttt atctatgaaa
     3001 gattatttcg atgtggaaat aataagacag aatatacaga tggttaattt aattgataaa
     3061 gccagtattg acaaagtgta taaaattaat tttttagtaa atgaaaggat ttagtaatga
     3121 ttccaattgt taaagttttt atgccaccta aagacgtttt gatgcctgct ttagaacagg
     3181 ttctttacag tggaatggtc ggtgaaggta agcatgttta tacctttgaa gataaattta
     3241 aaaagctctt tgggattaat aatgctattg ctgttagtag cggtactgcg gcattacaca
     3301 tggcattaat cttggctggt ataaaaaaag gcgatgaagt tattaccacc agcatgacag
     3361 cagaaccaac aaatacatca attttgcaag caggaggggt tcctgttttt gctgatgtag
     3421 ataaaaacac aggatgtctt gatcccgatt ctattattga gatgataagc gataaaacta
     3481 aagctatatt acttgtccat tatgcaggat atctggttga tgttcctgaa ctaaaggaaa
     3541 aattaagaag aaaaataaat agaaaaatta agattattga agactgtgca cacgctttag
     3601 gcgcgacaat tgatgggaga atggtaggaa ccttcggtga ctatggcata ttctcgtttc
     3661 aggctatcaa acatatgaca acaatcgatg gtgggtttct tgtaattaag gatacctctc
     3721 agacgaaatt ggctaaaaaa atgcgatggt ttggcatgga aaagggagtg gaaagaacat
     3781 ctcttgacct agatgttctg ggatttaaat ataatatgaa taatgttact ggtgttatag
     3841 gcgaaataca acttgattac gtaaaagata atatatctcg ccatcaaaaa aatgcccaat
     3901 tttataatat gatgctgaat gatattacgg gcatagaggt agtagaatct aggcaaagcc
     3961 actcaccgag ttactggctc tatactttat tatgtgatga ttctgatgat atcattaata
     4021 aattgaatga aataaatgtg gctgcttcga aactacatag gccaaaccat tatcattcac
     4081 ttctatcgca atgcagaagg gctagcatgg aaaatcttga ttcattctat aaaagattag
     4141 ttcatttacc ttgtggttgg tggatcgacg aaataacacg tagcaaaatt attgaagcat
     4201 tgagacgggg gtaatatgat taatttattc aatgtctcat ttgaccatga tattcagaaa
     4261 gtggtagaag acactctggt ttctaaggct attgcatcag gtgcacacgt atgtcagcta
     4321 gaaagtaggt tatctgattt attttcagaa aaatatgctc tgacagtaaa tgacatgact
     4381 aatggtataa ctttattatt agatgtcttt ggacttaaga aaggagatga agttttagta
     4441 tcacctttta gctgtttggc aacaacttca ccaatggcct tgttaggtat taaacctgta
     4501 tgggttgata ttgaaccatc aactctggct atgaatatta aatcaataga aagtaacata
     4561 acaaaaaaaa ctaaagcaat attggtatat cacgtggcag gttatcccag tgatattgtt
     4621 gagatttcta aaattgcgaa aaaaaataac ctattgttaa ttgaagattg taattctgca
     4681 tttggaagca agtatagaaa taaatcgctt ggttcttggg gggatgctac agttttatca
     4741 ttttatccaa atagacaagt tggttctatt gatggaggat gtgtactttt taaggataaa
     4801 aatcactatg aactggcgaa attaaggaga cgctatggag ttgattttaa tgatttccgt
     4861 aaagtaaatg gagaaattaa tgaagctaaa gatgtagaga cttatggcta ttcagcaaca
     4921 attaataacg ttagtgctgg aattattatg gccaaactac ctaagtatgc taatgaaatg
     4981 ttgctcaccc aaaaaaatgt atcgaaattg aataatgttg ttatgtctct acaaaataaa
     5041 tacaaaggaa gtattaagct ggtgacacct ttaaaggact catgcgttaa taattgggtt
     5101 tattttgttc gtgtaaggaa taaggaatct ttcatgaatg aaatgaaaaa gaaaggagtg
     5161 catgtatcgt ctttgcattt aaggaatgat ctgtattcat gttttcatgc agaaagaaaa
     5221 catttaacag gggttgatat tatatcagat acactagttg caataccttg tgggtattgg
     5281 atatcagaag aagacatcga tattattgga aaatatatag aacaaacatt aatatttttg
     5341 gttaaaaatg aaacatcgta taattgattt ggggatgtta tattttggca agtttggcgc
     5401 gttggcagtt ggtttttttc ttatgcctgc gtatagttat taccttggcc ctgcttcctt
     5461 ttcaactgtc gcctttatcc tttcgttagt taatgcagcc gtcacattag attttggcat
     5521 gagtactata attggaagag attcttctga tagtacaatt agtaatgaca agaagtataa
     5581 acaattttta gcatcacaat ttcttgtgat atttttttat ttatctgtat tttccattgt
     5641 tgctatatac tcttttcttt tccaaaaagg agatttgggg gtaaagataa gttttctgtc
     5701 agttattctt atcatgtcat ctctaattca gaatatttcc gtatcttatc tgaatggaat
     5761 taaagaattt aaaattagtg gttctcttat ttttttgtca gttacattac gaggggtaat
     5821 aagtctttta gtaataaaat atattgataa taatattata tcatttatgt ctgctcagtc
     5881 tataataggg cttttatttt ctttgatttt tatgctttta ccttctagac tggataaaga
     5941 aataaaaata aataatagtt ggggtggttt ttttgaacct tcaatctata taaaattttt
     6001 gctacctttg gtaaagaaag gtttcccttt gctttttatg ggagtatcgg ctacattggt
     6061 tatgcagttt gataaaatta ttctttcata ttacgctagc gatgcaacgc tttcagccta
     6121 ttacttagca tttactttca gcacaatacc tatacttgcg atagctggac ctattaaaca
     6181 atattttcaa cctcatatag ttagttattt aactcaaaat aatgtgctgt acaggaaaaa
     6241 tagcattgtc tttttttggt gcctgattgt ttttgttgca gtaccttcag cattaggata
     6301 ttcatcgtta taccaaatat tatctatttg gcttggaaat aatgagttga ttgataaagt
     6361 agactttttt tcacatatcc tgttgcctgc attcgttttg ggcgctataa gttattttcc
     6421 gtctgtatta ttagtggttg cagaggatta tagatttcag tctgtatttg caattatcac
     6481 gtctattatc tttgtttttt tattatttat acttgctata agtagtaatg ttcgtttcat
     6541 accatggttg tttataggtt atttttgtgt ggttatttta ttttgcagta taagatgttt
     6601 tagtttaact aaagtcaatt tatgtatatt tgatatggta aagaacagtc cttttgcaat
     6661 tctaataatt gcgctgtcct attgggttgg taatattgta tcagcaatga ttttttaatg
     6721 attgcttata ttatctatat atatcaatgt taattaaagc tgggattaat aatgtttaaa
     6781 gataagactc taatgattac tggtggtact ggatcatttg ggaatactgt gctgaagcgc
     6841 tttcttaata cagatgtaaa acgaattatt gtttttagtc gagatgagaa aaaacaagaa
     6901 gatatgcgga ttgctctcgc aaacccaaaa ctgaaattta ttattggtga tactcgtgat
     6961 tatgaatcag tataccgcgc aatgaatggg gttgattatg tattccatgc tgcagcatta
     7021 aaacaagttc catcttgtga attttacccg atggaagctg ttaaaactaa cgttaatggg
     7081 actgaaaatg tattaaatgc agctattgat tgtggcgtaa aacgtgttgt tttacttagt
     7141 acagataaag ccgtatatcc tataaatgcg atgggaatat caaaagcgat ggcggaaaag
     7201 gtgctaattg caaaagctag acggcaggat aacgacaaaa caatcatgtg tgcaacccgt
     7261 tatgggaatg ttatggcatc ccgtggctca gtaataccat tgttttttga aaaaataaaa
     7321 ctcaataaac cattgactgt aactgatcct aatatgacaa ggttcctcat gtcccttgaa
     7381 gattctgttg atcttgttct tcatgcattt aaaaatggaa gacaaggcga tatttttgtt
     7441 caaaaggcac ctgcttgtac cattggggac ttaacagagg ccataaaaca gttatgtgac
     7501 agtaacatag aaactaaaat aatagggaca cgtcacggtg aaaaacttta tgagtcgctg
     7561 gtttctcgag aagaaatggt aaaagctgaa gatatgggac gatattatcg cattccagcg
     7621 gataatcgag acttaaatta tgataaatat attattaaag gtcagataga tatcaataaa
     7681 cttgatgact atacttcaca taacactgaa cggttgaatg ttgaacaaat taaggcgttg
     7741 cttttgacat tgaaatatgt tcaaaaggaa cttggcattt aaataaattg ttatgttgca
     7801 aatccaataa taatattgga tttgcttatc tgagtggttt atatgatcaa acttagtatc
     7861 ttaataccta cgaaaaatcg tagtgagtac ttggatgatg ttttatcgag tttattgact
     7921 tggaaacgag atgattttga agttattatt caagataata gcgatgattt gaaaacacat
     7981 aatgttgtag aaaaatacag atatgatcat cgcgttaagt atttttatgt taaagaacat
     8041 ctatctgtga ttgataattt tgagtatgcc gtaaaaaata gctcaggaaa gatcattaca
     8101 gcaattggtg atgatgatgg aatattacct attgtaatgg atgttgcgga ttggatgctg
     8161 aaaaattcag tagaagcggt tttaactaat aaagcggagt acgtgtggcc tgatcttata
     8221 agcaagtaca actcgaatta taataatagt aaactcaaat ttaataaaaa ttattcttat
     8281 gattatraaa aaattaattg tatcgatgag ttcttgcagg tttgtcgcca cggaggtacc
     8341 agtatgggaa ggttaccacg catatattat ggccttgtct gtagggaatc ccttgaccat
     8401 gtatttaatc taacaggaca atatttcccc ggaccaagcc ctgatatggc taatgcagca
     8461 agtttgagtt tagttataaa gagtttctat tatttaaatt ttccagtttt tattgctggt
     8521 tcatcctcaa aaagtacagc cggtttgggg ttaaaacgta agcatcttgg tgctatatcc
     8581 gaaattaaac acctacctat gaatacaatg gctgagtgga ataaaaatat acccgatttt
     8641 tggtctggtc ctactatatg ggcacaaagt atttcaagtg taataaaaaa aatggagcca
     8701 acttatgatt taaattatga atatctatat gcccgatgtt ttgtattttt tcctcagaaa
     8761 agcaaatgta tttatttagc attaagacaa tatggtatat caggaaaaac tgttatcaaa
     8821 ataatatatt ggtatgtata taattggaaa ttaagatttg ttcatttgtt acctaagctt
     8881 ttagctaaga tagggaaaaa agatcataac attatcacta ttgatgaaca attaaaaata
     8941 tcacaggcag ttgactcgtt actttctttt actcaaaaaa gtgatttaag caagtgagtg
     9001 aaaatatatt atccagattg acattaaata gtgcccagaa atgtctactg acaggattaa
     9061 ttattatact tttggggccg tatggtgggg cgttgggatt tgctctggtt gtctgtgggg
     9121 caggattaga aaaaggtcga attgaaagat atattttatg tgttttttct ttcttcatat
     9181 ttacattatg ctcagtacag ggcgtatttc actcttctaa ttttaatact atatctttat
     9241 tcaccgcatt atgtacggtg atggttctat tcttggtgga taacaattca ttaatattga
     9301 ttctggataa gttaatattc gtaatatctg taactgttcc aatagctctg attattgcca
     9361 tttcacagac tttaggtgta tatccttttg aatttaggag taatgaagtt cttggagtaa
     9421 tgcgatttac atttttattc gccgaaccat ctcattattc tatacttttg tctttatgct
     9481 ctttaatttc tttggtgaaa aagacaaatt tatttacaag gtttattctt ttatttggac
     9541 ttttgatgac atggtcacta tctggtttct gtatttttac ttttggttat attgcttata
     9601 agttttatac aagaggcata ataaaatatt ttatcatttc actgcttctc ggatctataa
     9661 ttactttttt atggtttttt aaattacaat atagtgattt ctggttggtt gctaaaattg
     9721 attcaataat tcaattgttc tcaggtaaca cagttggtgc aagcagtgca cttcttagat
     9781 ttaactcgat tattattggc ccatatttca ttaatgagaa aattaactca gataattttc
     9841 ttttatcctt ttttggatta ggttttggaa acttatctga ctttgtctcg gtatattatc
     9901 aatctaattt tggattgcca gacgtctatg atgctaataa tattatttca aatgttataa
     9961 tatcaagtgg tatagttgga ttggttttct atatcctttc ctttagctac ttcactggat
    10021 taaacttagt taatatattg ttcgcagcta taatattact atgtttgtct ttgtttagtg
    10081 gctatgcatt cggaccattt gcgattctta ctttactttt atcaagagct tttattttat
    10141 tggttataaa tcatgacaaa gcacgcatca atcgatgtac ttatttcaac acatggttcg
    10201 agaataaaac aggttcctaa tatattacat gattttaaaa gaaatgttcg ctatataatc
    10261 tgtcaccaga attttgaact atatgaacca aacgagattt tgttaaatag aactgatgta
    10321 atatatatta ggtcaacaag taatggggtg actaaaagta gaaatattct tttagataaa
    10381 gcagatgcag atttaattta tttttgtgat gatgatgttg tattaagtga aaattttgat
    10441 gagattatta taaatagtca taaaaaatat tctgatgatg taattctttt taacattcgg
    10501 gatgaaaatg gtgaacttcg taaaaaatat ccaaatgaag tagtaaaaaa aacgcgattt
    10561 aatattttat ctgttggtac aattgaaata tcgctaaaga acagcccgtt attacctaaa
    10621 tttccggagg atatgggggc agggacatca ttacctgtgg gggatgaggc cgtttttttg
    10681 tctaccttta tcaaaaaagg gaagagtata cgttatatac ctaatacaat agctattcat
    10741 ccaagagaat caaccggtct agaggtaact aatggttcta tctactctag aggtgttgct
    10801 cttaatcgta tttatggtat ctatgcattt cttgtggcta ttttctttct gttgagaaga
    10861 aggagccttt taaaaactga tagaggagtt ttgcaggggg gtattttatt ttttaaaggc
    10921 gtcttactcg gtaagtaaaa aggaatagtt gtgaaaatat ctattgtaac agcaacatat
    10981 aattcatggc catatattaa ggaaacttat gattcaatcg caaatcaatc ttataaaaat
    11041 tgggaatggc atgtaacaga cgattgttcg tctgattata cctatcaata tctaaaacaa
    11101 ttagctgata gagattgtag aattgtaatt catcgaaatg agattaatag tggagcggca
    11161 gttgcaagaa ataaatcgtt atcgcaatgc tctggagatt atatcgcttt tatagatagc
    11221 gatgaccttt ggaacccaga gaaactcgaa agccaattga aatttatggt tgataaccaa
    11281 ttagattttt catttacggg atatgaggtt attgatgaat ctggcaagtc aataaaaaaa
    11341 tatattgaca ttaatcaaac acaagctatt agctacgaag atatgctaaa gaaaaaagca
    11401 acgttgggtt gctcaactgt tatgcttcgt aggctggctt tctccgagct ttccatgcct
    11461 gatcttcgca ctggtcagga ttatgcatta tggttaaaat tattaaaaac tggaattaga
    11521 gcagttcctt ataataaatc tttgatgaaa tatcgtatag tttcctcctc tatttcacga
    11581 aataaatatc gtaaagcaaa acgacaatgg caaatatacc gcgagctaga aaaactttct
    11641 ttggttaagt ctatctattg ttttttattt tatgcttgga gagctatttt taggaaataa
    11701 gtcaatttta tttgccattt ttctaaaagg aattaaaata ttgagaagtg taaataataa
    11761 cgagtagtca tcaataaata atagagttat taacttaccg catatcatgt ggtaccccct
    11821 gacaggagta aacaatgtca aagcaacaga tcggcgtcgt cggtatggca gtgatggggc
    11881 gcaaccttgc gctcaacatc gaaagccgtg gttataccgt ctctattttc aaccgttccc
    11941 gtgagaaaac ggaagaagtg attgccgaaa atccgggcaa gaaactggtt ccttactata
    12001 cggtgaaaga gtttgttgaa tctctggaaa cgcctcgtcg catcctgtta atggtgaaag
    12061 caggtgcagg cacggatgct gctattgatt ccctcaagcc atacctcgat aaaggtgaca
    12121 tcatcattga tggtggtaat accttcttcc aggacaccat tcgtcgtaac cgtgagcttt
    12181 ctgccgaagg ctttaatttt atcggtaccg gtgtctccgg tggtgaagaa ggcgcgctga
    12241 aaggtccttc cattatgcct ggtgggcaga aagaagccta tgaactggtt gctccgatcc
    12301 tgaccaaaat cgccgcagtg gctgaagacg gcgagccatg cgttacctat attggtgccg
    12361 atggtgcagg tcactatgtg aagatggttc acaacggtat tgaatacggc gatatgcagc
    12421 tgattgctga agcctattct ctgcttaaag gtggcctgaa tctttccaac gaagaactgg
    12481 cgcagacgtt taccgagtgg aataacggtg aactgagcag ctacctgatc gacatcacca
    12541 aagacatctt caccaaaaaa gatgaagacg gtaactacct ggttgatgtg attctggatg
    12601 aagcagcaaa caaaggtacc ggtaaatgga ccagccagag cgcgctggat ctcggcgaac
    12661 cgctgtcgct gattaccgag tctgtgtttg cacgttatat ctcttctctg aaagatcagc
    12721 gtgttgccgc atctaaagtt ctctctggcc cacaagcgca gccagctggc gacaaggctg
    12781 agttcatcga aaaagttcgc cgtgcactgt atctgggcaa aatcgtttct tacgctcagg
    12841 gcttctctca gctacgcgcc gcgtctgaag agtacaactg ggatctgaac tacggcgaaa
    12901 tcgcgaagat tttccgtgct ggctgcatca tccgtgcgca gttcctgcag aaaatcaccg
    12961 atgcctatgc cgaaaatccg cagatcgcta acctgctgct ggctccgtac ttcaagcaaa
    13021 ttgccgatga ctaccagcag gcgctgcgtg atgtcgttgc ttatgcggta cagaacggta
    13081 tcccggttcc gaccttctct gcggcagttg cctattatga cagctaccgc gccgctgttc
    13141 tgcctgcgaa cctgatccag gcacagcgtg actatttcgg tgcgcatact tataagcgca
    13201 ttgataaaga aggtgtgttc cataccgaat ggctggatta atctgattta aatcaattaa
    13261 tcaatgcaag gcccggagaa accctccggg cttttttatt atacaaagcg gcaggttatg
    13321 gccttttttt ataatttata gttaaaaacg cgatataata cagcgccgca cagcaggatc
    13381 gctgccttga cagttcatca acatcagcgt taaaaatccc gcagtagatg aagttgtggt
    13441 ggtggattaa tgaccactct aaatgtttaa ccggaagaag tcagagctaa tgaaaataac
    13501 aatttcagga acaggttatg ttggtctttc aaatggtatt ctgattgcgc aaaatcacga
    13561 agtggttgca ctggatatcg ttcaggccaa agtggacatg cttaacaaga agcagtcacc
    13621 gattgttgat aaggagattg aagagtatct ggcgactaaa gatctcaatt tccgcgctac
    13681 gacagataag tatgacgcgt ataaaaatgc cgattacgtt attattgcca cacctaccga
    13741 ttatgatccg aaaacgaatt attttaatac ctcaagcgtg gaagcggtca ttcgcgatgt
    13801 gacagaaatt aatcctaatg ctgtcatggt gattaaatct acaattccgg taggtttcac
    13861 caaatcaatt aaagaacgtc tgggtatcga taaccttatt ttctctccgg aattcctccg
    13921 cgaaggtaaa gctctttacg ataaccttca tccgtcacgt attgtcatcg gtgagcgttc
    13981 agaacgcgca gaatgttttg cggcgttatt acaggaaggc gcgattaagc aaaatatccc
    14041 tacactgttt accgactcca ctgaagcaga agcgattaaa ctgtttgcta atacctatct
    14101 ggcgatgcgc gtagcatact ttaatgaact ggatagctat gcagaaagtt tagggctgaa
    14161 tactcgccag attatcgaag gcgtttgtct cgatccgcgt attggcaacc attacaacaa
    14221 cccgtcgttt ggctatggtg gttattgtct gccgaaagat accaagcagt tactggcgaa
    14281 ctaccagtct gtgccgaata acctgatttc ggcaattgtc gatgcaaacc gcacgcgtaa
    14341 agactttatt gccgatgcca ttttgtcacg caaaccgcaa gtggtgggta tttatcgtct
    14401 gattatgaag agcgggtcag ataacttccg cgcgtcttcc attcagggga ttatgaaacg
    14461 tattaaggcg aaaggcgttg aagtgatcat atacgagcca gtgatgaaag aagactcatt
    14521 cttcaactct cgcctggaac gagaactcgc caccttcaaa caacaagccg acgtcattat
    14581 ttccaaccgt atggcagaag agcttaaaga tgtggcagat aaggtctata cccgagatct
    14641 ctttggcagc gactaacatc ctgtgatcag ggcgattttc gccctgatta tcttttgtta
    14701 cttacggaac aattcattat ttttatcact tatcctatag cattcacgag gattatcgct
    14761 aaactatggc gacttaaaat tatttcgtcc gttagggtaa tgatgagagt agaaaataat
    14821 aatgtttctg ggcaaaacca tgacccggaa cagattgatt tgattgattt actggtgcag
    14881 ttgtggcgcg gcaagatgac aattatcatc tctgtcattg ttgctattgc cctggctata
    14941 gggtatctgg ccgttgccaa agaaaagtgg acttccacag ctatcgttac tcaacctgat
    15001 gtggggcaaa tcgctggtta taccaacgcg atgaatgtga tttacggtcc ggctgtaccg
    15061 aaagtctccg acattcaggc ttcacttatc ggacgttaca gcactgcatt ttcagcatta
    15121 gcggaaacgc tggataacca ggaagaagca gagaagctca ccattgagcc taccgtcaaa
    15181 aatcagtcat tacctttagc ggtgtcttat gttggtgaaa cacctgaagg tgcgcagaaa
    15241 cagctggcga agtatatcca gcaagttgac gaccaggtaa atgaagaact ggaaaaagac
    15301 ctgaaagaca acatcgcgct gcgtatgaaa aacttgcagg attctctgaa aactcaggaa
    15361 gtagttgcgc aggagcagaa agatctgcgt atccgtcaga ttcaggaagc gttgcagtat
    15421 gcgaatcagg cgcaggtgac aaagccgcag attcaacaga cgcaggacgt gacgcaagat
    15481 acgatgttcc tgctgggaag tgacgcgctg gagtcgatga ttaagcatga agcgacccgc
    15541 ccactagttt tctcgccaaa ctactatcag actcgtcaaa acctgctgga tatcgaaagt
    15601 ttaaaggttg atgatcttga tatccatgct taccgctatg tgatgaaacc gacgttacct
    15661 attcgtcgcg atagcccgaa aaaggcaatt accttgattc tggcggtact gctgggcggc
    15721 atggttggcg cggggattgt gctggggcgt aatgctctac gcaattacaa cgcgaaataa
    15781 tctttgcagc tttaaagaaa acgggcaggg tggtgacacc ttgcccgttt ttttttgccg
    15841 gatgcgacaa caatatcgca tccgcttacc ccgcaactca ctgatgccgt ttacgcaggt
    15901 tctcaatcac cgtcgttaaa tccagatcct gatcctgcaa caataccaac aggtgataca
    15961 tcaaatcaga cgcctcgttg gtcagctcaa agcggtcatg tacagtcgcg gccagtgcgg
    16021 tttccacgcc ttcttcgccc actttctgcg caatgcgttt ggtgccgctg gcatacagtt
    16081 tggcggtgta ggaggtttcc gggtcggcag atttacgttc ggcgagcagt tgttccagtt
    16141 gatacaggaa cagccactgg tgagcggtgt cgccgaagca gctgctggtg cctttgtggc
    16201 aggttggccc gattggattt gccagcacca gtaacgtgtc gttgtcgcag tccggggcaa
    16261 tactcaccac attgaggaaa ttgcccgacg tttcgccttt ggtccacagt cgctgtttag
    16321 tgcgcgagaa gaaggtgact ttgccgcttt cgatggtttt gtctaaggct tccgggttca
    16381 tatagcccag cattaacact tcgccggata ccgcgtgttg cacaatcacc ggcatcagtc
    16441 cgtcggtttt ttcccagtcc agttcgcgac gttgttgttc tgttaacata tcctgatctc
    16501 cacgccctgt gttgccaggt acgcttttaa ttcaccaata ttgattattt gtttgtggaa
    16561 tacggaagct gccagcgcgc cgtcaacgtc ggcatcacgg aaggcttcga ggaagtgttc
    16621 catggtgccc gcgccaccgg aggcaattag cgggacgtgg caaacttcac gtactttttt
    16681 cagttgttcg aggtcgtaac cgttacgcac gccgtcctga ttcatcatgt tgaggacgat
    16741 ttctccggca ccgcgttttt gcacttcctg tacccagtcg agcgtttccc actgagtgac
    16801 gcgggtgcgg
//
