LOCUS       AB812081               16331 bp    DNA     linear   BCT 13-DEC-2014
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: 99-6301.
ACCESSION   AB812081
VERSION     AB812081.1  GI:733167535
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. (2014) In press
   PUBMED   25428893
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16331)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..16331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="99-6301"
                     /db_xref="taxon:562"
                     /note="serogroup:O185"
     gene            <1..906
                     /gene="wcaM"
     CDS             <1..906
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ02042.1"
                     /db_xref="GI:733167536"
                     /translation="NYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILI
                     SDHLIERINCTNGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVE
                     NGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVI
                     KGKYLSIPQNFRVNNIQLDNTYLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNK
                     PQHLFMRNINVMQESTVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSS
                     VDIDRINHHIVNVEKINFRLPERRE"
     gene            1064..2059
                     /gene="gne"
     CDS             1064..2059
                     /gene="gnu"
                     /note="O185_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAQ02043.1"
                     /db_xref="GI:733167537"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPSERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            2302..3195
                     /gene="galF"
     CDS             2302..3195
                     /gene="galF"
                     /note="O185_02"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ02044.1"
                     /db_xref="GI:733167538"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKAIEDTLLK"
     CDS             3356..4174
                     /note="O185_03"
                     /gene="wbwC"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ02045.1"
                     /db_xref="GI:733167539"
                     /translation="MQKIEKISVLLSIYFKENEIYLDEAFKSLAIQKTMIDEVVAVID
                     GPISNGLWRVVRKWDRELPLKIVELNENVGLGKALNAGLQACSNDLVARMDTDDICLP
                     ERFEKQIKMFIRNPELVIIGGAVDEFTEKNNIRKLKKVPCGYDNISKTIAKRNPFNHM
                     TVMFRKQKIIEIGGYNHHYLMEDYNLWLRCYAAGFAMDNVNDVLVLARAGEEMINRRK
                     GLKYIKSEYELYKLIVDLGINKGVSAKLIFLGRSLVRLLPKQLLSSIYNQFLRK"
     CDS             4171..4776
                     /note="O185_04"
                     /gene="wbaI"
                     /codon_start=1
                     /transl_table=11
                     /product="putative phosphatase"
                     /protein_id="BAQ02046.1"
                     /db_xref="GI:733167540"
                     /translation="MKKKILLLDLCGSLVKENTTFAFLERFDYVRRKSLIFISLYLIY
                     KFTKFDLLRRWAIAKLKGVPKTILRKQAKIFVKECHYNDAVLNLIEKEDFDEIYIVSA
                     TLDFIAEEFYSELNYNGFYATCLDYLDSVATGKIKVDLLNNKENIINIISDVNKSFTM
                     ISDNFGDLNMMKKCDVAIAVANNKKAVVYWVRNGIEKIIKV"
     CDS             4778..5551
                     /note="O185_05"
                     /gene="wbaJ"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ02047.1"
                     /db_xref="GI:733167541"
                     /translation="MWCKFYIPFLYFIETRTKTRAKTISWCLIYLFPVLLLFFLFEST
                     KESVELWGQLGLTIVLTYNLYELGYIYNDAETIKKEIKPTLRLSEKEIRYYEGNKKTI
                     YIFRLFVSVVVTFCVYKLGGEFLWYPWLLIPMYAIYNSIRNRLNLLLHFILVTIRYSS
                     PLVVCTGDLRSIIIFTICFSIINVIERCGEKRFELYYFQRELFKNHSLLRCRYYLFIF
                     FTSLCVYLNSLSELSLLIMVMSLYYFVYRLMIFFMEKIK"
     gene            5548..6918
                     /gene="wzy"
     CDS             5548..6918
                     /gene="wzy"
                     /note="O185_06"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ02048.1"
                     /db_xref="GI:733167542"
                     /translation="MNNSAIIVFVDSIIIAFFIVGYLLNEAGFVSVDYYTLAISCFLF
                     IAFAYLQTALLSGVLSIISLFIMTISIFIFGRIFSYLLYSEMDYYTVTLFYTTTISSL
                     DSVNLLLTIGIIIYSILLGYFFTQCIENKNKNVNVVTYNLRLPNGPVLFLVGCLVFPY
                     IYSGLEAVNSGGYLSFYTKNLELLEKGSIIPNLYSLGITIATVFLSISISTDNKKNTN
                     FYLCVLLMNSLMLAFIGQRGPLLSFFLFYLWYRNKYIKKIPLLRLVIIGGIAIVFSQT
                     LLLFRADYSNSNINLFEHYFSFLFQQGVTLHVIYFTKYIDNFPGYLLAQNFIPGANAI
                     YNMFAEHTILYNSSSAYLSDLLNPALFKSGYGVGWSFVNDIVLLCNKNVGAMIIVACL
                     FGVFLSLAENRGNSKGLYITALSVITAYRIGFLPRAGLNGIFPLWIYSLVIITLLMVL
                     KKKWKN"
     CDS             6906..7802
                     /note="O185_07"
                     /gene="wcaA"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ02049.1"
                     /db_xref="GI:733167543"
                     /translation="MEKLVSVLITSYNRPLLLRRAILSVFNQTYSKIELIVVDDNSSY
                     EVDLIVKELQEVATPNIKNFIYKKNPRNMGANFSRNRGLKHAKGFFITGLDDDDYFKE
                     DRLQILVNHYNKEFSFICDNYIIVTGTNNHARFQRECIIDLQSILKENEAGNQILVER
                     ERLLSVGGFSIDFKRLQDQDVWTKLIQVYGSARRLNANTYYMDISHEGVRITKNIKDY
                     VAYRKYYFKYKNIMYNDTKNYNLFRLMMLRCLKIKIIFSFIAKKQYWTLFATKLLLKR
                     AFPGIIYLKQKVYENHFKKRVR"
     gene            7810..9243
                     /gene="wzx"
     CDS             7810..9243
                     /gene="wzx"
                     /note="O185_08"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ02050.1"
                     /db_xref="GI:733167544"
                     /translation="MLLITGVKWVALSQIARALSQLVLIFVLARYLTPYDYGLISIVS
                     VVAAFAMLFRDMGTSSILIQRRRISENFIKSTYTLNFISGLVIFIVMTLLAYPISLFY
                     NDTRLFLLILIISFSFPLQSFIAFNFALLERNQQFNITALIELISLSISIIVVFIVIA
                     MGFGLYAVSLQALLYAVIVVFLLSLKNRRKYKFSWRMKYIKLIFGYCVNVMSFNMVNY
                     FSRNADNIIVGKIFGQYVLGNYALAYRLMLFPVQNITYIFNRALLPKLSHSKDIITKK
                     RIYLDSLKFIAVLSIPAMIGLASLSNELVFFLFGKNWDNAALLLKWLAPVGIIQALTS
                     TTGVVFLSLGNVKLLARIGCVSAIIQVMSFVIGACFNIVIFCQLYLCANVLIFYICFY
                     YVMRLLQGNIKNILCITCKPLFCSVCMLILILIAKNNFITQSNNMELLSLIYYIVIGA
                     GSYVCLMFIIDKNTIVGLLNNALPKRK"
     CDS             9221..10198
                     /note="O185_09"
                     /gene="wbaK"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ02051.1"
                     /db_xref="GI:733167545"
                     /translation="MLCQKENEISKLISIVMPVYNVQDYIVEAIDSIINQSMQPHSVI
                     IVDDGSSDDSIPLLKKRIEGKDNYKIFSKENGGLSSARNYGLTKVSTPYVYFFDSDDA
                     LSPMFIRDINEILTNYKELDIIAFSGSILGLPSNLAGKIPSYKRTKFQICKSGDVFLE
                     RLFSTNSYFSSVCINVFRTELLMRNSFFKNIVHEDEEYLPRIILDSKLCYCTDEIYFY
                     RRVRENSIMTSKISSRNIIGYLEVLKTYRCIIQTTKNRLVKYYLKKRYNDFLIYIFLL
                     CKKNKIVLPKNIEQKMNCEQAFGMYYAFRLLLIKKFFPVYHFLYQVKNR"
     CDS             10232..11248
                     /note="O185_10"
                     /gene="wbaL"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-glucose 4-epimerase"
                     /protein_id="BAQ02052.1"
                     /db_xref="GI:733167546"
                     /translation="MAVLVTGGAGYIGSHTVLQLLNHNYDVIVIDNFSNSSKIALKRV
                     EYLTSKKIIIYEGDIRNIDLIDSVFANNDIEAVIHFAALKSVSESIKKPIQYYENNVI
                     GTLHLIDSMLKNKIYNFIFSSSATVYGNPTAIPVHENCSTGGTTNPYGTTKYIVERML
                     EDITNYEFKFNATCLRYFNPVGAHYSGLIGEDPNGVPSNLFPYLTQVAVGKLEKLTIY
                     GGDYPTVDGTGVRDYIHVMDLAEGHIAALKKQNIYGGLRYYNLGTGKGYSVKEIIDAF
                     QKITGIKIPYVISERRLGDIAECWSKPDLAIKELHWEAKRTLEDMITDAWRWQNKNPN
                     GYSE"
     gene            11356..12762
                     /gene="gnd"
     CDS             11356..12762
                     /gene="gnd"
                     /note="O185_11"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ02053.1"
                     /db_xref="GI:733167547"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            13011..14177
                     /gene="ugd"
     CDS             13011..14177
                     /gene="ugd"
                     /note="O185_12"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ02054.1"
                     /db_xref="GI:733167548"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            14267..15301
                     /gene="wzz"
     CDS             14267..15301
                     /gene="wzz"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ02055.1"
                     /db_xref="GI:733167549"
                     /translation="MVIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(15396..16007)
                     /gene="hisI"
     CDS             complement(15396..16007)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ02056.1"
                     /db_xref="GI:733167550"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALGK
                     TLESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     4928 a   2866 c   3448 g   5089 t
ORIGIN
        1 aattacgcca tcttacgcca gggatttcat aaccagataa tcggtgccaa catcaccaac
       61 tgtaagttca gcgacttaca gggcgacgct attgaatgga acgtggcgat taacgacagt
      121 gatattttga tatccgacca tctcattgag cgcattaact gtaccaacgg caaaatcaac
      181 tggggcatcg gcataggcct tgcgggaagt acctacgata ataattatcc ggaaaaccag
      241 gcagtgaaaa actttgtcgt ggcgaatatc acgggatcgg attgtcggca actgatccat
      301 gttgaaaatg gtaaacattt tgttattcgt aatatcaagg cccgcaatat cacaccggat
      361 ttcagtaaga aagcaggcat tgataacgcg acagtcgcta tttacggttg tgacaatttc
      421 gtgattgata atattgaaat gattaatagc gccgggatgt taatcggtta tggggtaatt
      481 aaaggcaaat atctctcgat accgcagaat ttccgggtga ataatattca actggataac
      541 acctaccttg cttataaatt gcgcggcatc caaatctctg ccgggaatgc tgtctccttt
      601 gtggcgctga ctaacattga gatgaagcgt gcgtcgctgg agcttcacaa taaaccgcaa
      661 catcttttta tgcgaaatat caatgtgatg caggaatcca ctgttggacc cgcactgagc
      721 atgaacttcg acatgcgcaa agacgtacga ggtgtcttta tggcgaaaaa agaaacactc
      781 ctgtctcttg caaatgttca tgcggtgaat gaaaaaggac aaagctctgt cgatatcgac
      841 aggattaatc accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg
      901 gagtagattt gcgaccattc ataaacttac tggatataat gttctaaaag attataatcc
      961 tcacactaac tgttaaagct ggggctgggc agcattgact actcagggaa ggtactcatc
     1021 tttatcttgt gtttcttacg taacaaccag actaaagtga tttatgaacg ataacgtttt
     1081 gctcatagga gcttccggat tcgtaggaac ccgactactt gaaacggcaa ttgctgactt
     1141 taatatcaag aacctggaca aacagcagag ccacttttat ccagaaatca cacagattgg
     1201 cgatgttcgt gatcaacagg cactcgacca ggcgttagcc ggttttgaca ctgttgtgct
     1261 actggcagcg gaacaccgcg atgacgtcag ccctacttct ctctattatg atgtcaatgt
     1321 tcagggtacc cgcaatgtgc tggcggccat ggaaaaaaat ggcgttaaaa atatcatctt
     1381 taccagttcc gttgctgttt atggtttgaa caaacacaac cctgacgaaa accatccaca
     1441 cgaccctttc aaccactacg gcaaaagtaa gtggcaggcg gaggaagtgc tgcgtgaatg
     1501 gtataacaaa gcaccatcag aacgttcatt aactatcatc cgtcctaccg ttatcttcgg
     1561 tgaacgcaac cgcggtaacg tctataactt gctgaaacag atcgctggcg gcaagtttat
     1621 gatggtggga gcagggacta actataagtc catggcttat gttggaaaca ttgttgagtt
     1681 tatcaagtac aaactgaaga atgttgccgc aggttatgag gtttataact acgttgataa
     1741 gccagacctg aacatgaacc agttggttgc tgaagttgaa caaagcctga acaaaaagat
     1801 cccttctatg cacttgcctt acccactagg aatgctgggt ggatattgtt ttgatatcct
     1861 gagcaaaatt acgggcaaaa aatacgctgt cagctctgtg cgcgtgaaaa aattctgcgc
     1921 aactacacag tttgacgcaa cgaaagtgca ttcttcaggt tttgtggcac cgtacacgct
     1981 gtcgcaaggt ctggatcgaa ctctgcagta tgaattcgtc catgccaaaa aagacgacat
     2041 aacgtttgtt tctgagtaat gcagacgagt cagagaaaaa aggaccaggt tggactacga
     2101 ttattcggaa agaggtactg attaaattat cagtatgtgg atttccgagt aatccgagta
     2161 acccagatgc tgatggtgtg tagtgttatc gcaaggttta atcaaagtta tgcggaacta
     2221 agctttggta tatagccata acgtttggct aacctatggc aataatttac gcactatcag
     2281 ccacttagag gtaggaaaat aatgacgaat ttaaaagcag ttataccggt agcaggtctt
     2341 gggatgcata tgttgcctgc cactaaggcg attcccaaag agatgctacc gatcgtcgac
     2401 aagccaatga ttcagtacat tgttgacgag attgtggctg cagggatcaa agaaatcctc
     2461 ctggtaactc acgcgtccaa gaacgcggtc gaaaaccact tcgacacctc ttatgaatta
     2521 gaatctctcc ttgagcagcg cgtgaagcgt caactgttgg cggaagtaca atctatctgt
     2581 ccgccgggcg tgaccattat gaacgtgcgt cagggtgaac ctttaggttt aggccactcc
     2641 attttatgtg cgcgacctgc cattggtgac aacccatttg tcgtggtact gccagacgta
     2701 gtgatcgacg acgccagcgc cgacccgctg cgctacaacc ttgctgccat gattgcgcgc
     2761 ttcaacgaaa cgggccgcag tcaggtgctg gcaaaacgta tgccgggtga cctctctgaa
     2821 tactccgtca tccagaccaa agaaccactg gatcgcgaag gtaaagtcag ccgcatcgtc
     2881 gaatttatcg aaaaaccgga tcagccgcag acgctggact cagacatcat ggccgttggt
     2941 cgctatgtgc tttctgccga tatttggccg gaacttgaac gcactcaacc tggtgcatgg
     3001 gggcgtattc agctgactga tgctattgcc gaactggcga aaaaacagtc cgttgatgcc
     3061 atgctgatga ctggtgacag ctacgactgt ggtaaaaaaa tgggctatat gcaggcgttt
     3121 gtgaagtatg gattacgcaa cctgaaagaa ggggcgaagt tccgtaaagc tattgaagat
     3181 actttgctaa aataagtaag atttgcgtta gtttaatagc ggaaataggc ggtagtatga
     3241 aaattatgaa gcattttctc attatcgtct tagtttttta aatattaaca agtatttaaa
     3301 taatgtggca ttggtttttg agtgtgttgg tagctgttaa gccaagggcg gtagcgtgca
     3361 aaagatagaa aaaatatcag ttttattatc tatatatttt aaagaaaacg aaatttatct
     3421 tgatgaggca tttaaaagtc ttgcgataca aaaaacaatg atcgatgaag tagttgcagt
     3481 tattgacggt ccgatcagta atggattatg gagagtagtt agaaaatggg atagagaatt
     3541 gcctttaaaa atagttgaac tcaatgagaa tgttggatta ggtaaggcat taaatgctgg
     3601 cctacaggca tgttcgaatg atttggtcgc acgaatggat actgatgaca tttgtctacc
     3661 tgagcgattc gaaaaacaaa taaagatgtt tattcgtaat ccggaattag taataattgg
     3721 cggggcagtt gatgagttta cagaaaaaaa caatatacgt aaattaaaaa aagtaccatg
     3781 tggttatgat aatattagta aaacaatagc aaaacgaaat ccttttaatc acatgactgt
     3841 aatgtttaga aaacaaaaaa ttattgagat tggtggttat aaccaccatt atcttatgga
     3901 agactataac ttgtggcttc gttgttatgc cgcaggtttc gctatggata atgttaatga
     3961 tgtattagtt ttagcacgtg ccggcgaaga aatgatcaat agaagaaaag gattaaaata
     4021 tatcaaaagt gaatatgaac tttataaact tattgttgat ttaggcatta ataaaggggt
     4081 aagtgcaaaa ttaatatttt taggtcgttc attagtcagg ctattaccta aacaattatt
     4141 atcatctatc tataatcagt tcttacgcaa atgaagaaaa aaattctatt attggattta
     4201 tgtggatctt tggttaaaga aaatacgact tttgcttttc ttgaacgatt tgattatgtc
     4261 agaaggaaat ctcttatttt tatttcttta tatttaattt ataaattcac gaaatttgat
     4321 cttttaaggc gctgggcaat agcaaaactt aaaggtgttc ccaaaactat actgcgaaaa
     4381 caagctaaaa tatttgtgaa agaatgccat tataatgacg cggtattaaa tttaattgaa
     4441 aaggaagatt ttgatgagat atatatagtt tcagcaacgc ttgatttcat cgcggaagag
     4501 ttttattcag aacttaacta taatggtttt tatgctactt gcttggatta tcttgatagt
     4561 gttgcaactg ggaagataaa agtggattta ttaaataata aggaaaatat tataaatata
     4621 attagtgatg ttaataaatc attcacaatg ataagtgata attttggtga tcttaatatg
     4681 atgaaaaaat gcgatgtggc catagctgtg gctaataata aaaaagcagt tgtttattgg
     4741 gtaagaaatg gcattgaaaa aataattaag gtgtagtatg tggtgcaaat tttatatccc
     4801 atttttatat tttattgaaa caagaactaa aacgcgtgct aaaacaattt cttggtgcct
     4861 aatttatcta tttccagttt tgttgctgtt ttttttattt gaaagcacta aagagagtgt
     4921 tgagttatgg gggcaactgg gattaactat agtactaaca tataatctgt atgaattagg
     4981 atatatctac aatgatgcag aaacaataaa aaaagaaata aaacctactc tgcggctttc
     5041 tgaaaaagaa atacgctatt atgagggtaa taaaaagaca atatatatct ttagattatt
     5101 tgtatctgtt gttgtcactt tttgtgttta caagttaggt ggagagtttc tctggtatcc
     5161 atggttactt atcccgatgt atgcgatata taattcaata agaaatcgat taaatctttt
     5221 attgcatttt atattagtta caatcaggta ctcttcacca ttagttgttt gtacagggga
     5281 tttgcgtagc ataataatat ttacaatatg tttctcaatt ataaatgtga tcgagaggtg
     5341 tggtgagaaa agatttgagt tgtattattt tcagcgagag ttatttaaaa atcattcatt
     5401 gttaagatgc cgctattatc tttttatttt ttttacctca ttatgtgtat acttaaatag
     5461 cctttctgag cttagtctat taataatggt aatgtcttta tactactttg tttatcggct
     5521 tatgatattt tttatggaaa aaataaaatg aataattctg cgataatagt attcgtcgat
     5581 tctattatta ttgccttttt tattgttggt tatttattaa atgaggccgg ttttgttagt
     5641 gttgactatt acacattagc aataagttgt tttttattta tagcatttgc atatttacaa
     5701 acggcacttt tatcaggggt actaagcata atatcgcttt ttataatgac aataagtata
     5761 tttatttttg ggcgcatatt ttcatattta ttatactctg aaatggatta ctatacagta
     5821 acgctttttt ataccacgac tataagttca cttgattctg tgaatttgtt attgacgatt
     5881 gggataataa tatattcaat tcttttaggg tattttttta ctcagtgtat tgaaaataag
     5941 aataaaaacg tcaatgtagt aacatataat ctgaggttgc ctaatggacc agttttattt
     6001 ttggtcggtt gccttgtttt tccatatata tattcaggtt tagaggcagt aaattcaggc
     6061 ggatatcttt cattttatac aaaaaattta gaattattgg agaaaggaag tattatacca
     6121 aatctttatt ctttaggaat tacgatagcg actgtttttt tgtctatatc aatatcaact
     6181 gacaataaaa agaacactaa tttttatctg tgcgttttat tgatgaactc tttaatgttg
     6241 gcttttatcg gtcagcgagg tccattatta tcattctttt tattttatct ttggtataga
     6301 aataagtaca ttaagaaaat acccctcctt aggttagtta taataggtgg tattgctata
     6361 gttttctccc aaacattgtt attatttaga gcggattata gtaattcaaa tataaatttg
     6421 ttcgaacatt acttctcatt cttatttcaa caaggagtga cattacatgt catctatttc
     6481 actaaatata tagataattt tcctggctat ttattagctc aaaactttat ccctggagct
     6541 aatgctatat ataacatgtt tgcagaacac actatattat acaatagctc ttctgcgtat
     6601 ctatccgatt tattaaatcc agcgttattc aaaagtggtt atggtgttgg ctggagtttt
     6661 gtcaatgata tagttttgct gtgtaataaa aatgttggag ctatgattat tgttgcttgc
     6721 ttatttgggg tgtttttaag tttagcagaa aatagaggta actctaaggg gctttatatt
     6781 actgcattat cagttataac agcatatagg ataggctttt taccaagagc agggcttaat
     6841 ggcatattcc cgctgtggat ctattctttg gttataatta ctttgttgat ggttttgaaa
     6901 aagaaatgga aaaattagta agcgttctta taacatctta taatcgccct ttgctgttgc
     6961 gcagagcaat attatcagta tttaatcaaa cttattcaaa aatagaatta atcgtagtcg
     7021 atgataactc tagttatgaa gttgacttga ttgtaaaaga attgcaagaa gtagcaacac
     7081 caaatattaa gaattttata tataagaaaa acccaagaaa tatgggggct aatttttcta
     7141 gaaatagagg attaaagcat gcaaaaggtt tcttcattac agggttggat gatgatgatt
     7201 atttcaaaga agataggtta cagattctgg ttaatcatta taacaaggaa ttttccttta
     7261 tctgcgataa ctatataata gtgactggaa caaataatca tgcacgattt caaagagaat
     7321 gtatcattga tttacaaagt atactcaagg aaaatgaagc tggtaaccag attttagttg
     7381 agcgagagcg tttgttatca gttggaggat tttctattga ttttaaacgg ttacaagatc
     7441 aagatgtttg gactaaactt attcaagttt atgggagtgc caggaggtta aacgctaata
     7501 cttactatat ggatatttct catgaaggtg ttagaataac caagaatatt aaagattatg
     7561 tggcatatcg gaagtattat tttaaatata agaatataat gtacaatgat acaaagaatt
     7621 ataacctgtt taggttaatg atgttaagat gtttaaaaat taaaattata ttttctttta
     7681 tagcaaaaaa acaatactgg acattatttg caactaagtt gctactcaaa agagcgttcc
     7741 ctggcattat atatttgaag caaaaagttt atgaaaacca ttttaaaaaa agagtgagat
     7801 aataataaaa tgcttttgat aacaggagta aaatgggtcg ctttatctca aattgcaaga
     7861 gctctgagcc aattagtttt gatctttgta ctggctcgtt atttaacgcc ttatgactat
     7921 ggtttgattt ccattgtaag cgtagtggct gcatttgcca tgctctttag agatatgggg
     7981 acgtcatcaa tattaattca acggagaaga ataagtgaaa attttattaa atccacatac
     8041 acattaaact ttataagcgg tctcgttatt tttatcgtaa tgacattatt agcttatcca
     8101 atatcccttt tttacaatga cacaaggttg tttctattaa tattgatcat ctctttttca
     8161 tttcctttac aaagttttat tgcttttaat tttgctttat tagaacgaaa ccaacagttc
     8221 aatattactg ctctcataga gttgatatca ttatcgattt caatcattgt tgtttttata
     8281 gtaattgcca tgggctttgg actttatgct gtgtctctac aggccctttt gtatgcagtt
     8341 attgttgttt ttttattgag ccttaaaaat agaagaaaat ataaattttc ttggcggatg
     8401 aaatatataa aattaatatt tggctattgt gttaatgtaa tgtcatttaa tatggtgaat
     8461 tatttctcac gtaacgctga taatattata gttggaaaaa tttttgggca gtatgtttta
     8521 ggtaattatg cattagcata tagacttatg ttatttccag ttcaaaatat aacttatata
     8581 tttaatagag ctttattgcc taagctaagt cattcaaaag atattataac aaaaaaaaga
     8641 atatatttag actcgctaaa gttcattgca gttttgtcaa ttcctgccat gattgggctc
     8701 gcttcgttaa gtaacgagtt agtttttttt ctgtttggta aaaattggga taatgctgct
     8761 cttcttttaa aatggttggc acctgttggg attatccaag cactcacgag cactacagga
     8821 gtagtttttt tatctttagg taacgttaag ttgctggcaa ggattggttg tgtgagtgca
     8881 ataattcaag ttatgtcatt tgttattggg gcatgcttca atatcgttat attctgccag
     8941 ttatatcttt gtgcgaatgt attaatattt tacatctgct tttattacgt catgagattg
     9001 ctccaaggta acataaaaaa cattttatgt ataacatgta aacctttgtt ttgtagtgtt
     9061 tgcatgctaa ttttgatact gattgctaaa aataatttta taactcaatc taataatatg
     9121 gaattattat ctttgattta ttatatcgtt ataggtgcag ggagttatgt ttgtcttatg
     9181 tttattattg ataaaaatac cattgtggga ttattaaaca atgctttgcc aaaaagaaaa
     9241 tgagatttcc aaattaatat caattgttat gcctgtgtac aatgtccaag attatattgt
     9301 tgaggctata gattctatta taaatcagtc tatgcaacca cattcagtta tcatcgtgga
     9361 tgatggttct agtgatgatt cgatacctct ccttaaaaaa agaatagagg gtaaggataa
     9421 ttataagata ttttctaaag aaaatggtgg tttaagcagt gctaggaatt atggtctgac
     9481 aaaagttagc acaccatacg tctatttttt tgactctgat gatgctttga gtccaatgtt
     9541 tataagggat attaatgaaa tattgactaa ttataaagag ttagacatta tagcattttc
     9601 aggatcaata ttagggctcc cttctaattt ggcaggtaaa attccttcat ataaacgaac
     9661 taaatttcaa atttgtaagt ctggtgatgt ttttttagaa agattatttt caaccaattc
     9721 ttacttttca tctgtttgca ttaatgtatt tagaactgaa ttattaatgc gaaattcatt
     9781 ttttaaaaat atagttcatg aggatgaaga gtacttacct aggattattt tagattctaa
     9841 gttgtgttat tgtaccgatg agatttattt ttaccgaagg gtaagggaaa actcaataat
     9901 gacaagtaaa attagctctc gcaatataat tggttatctg gaagtattaa aaacctatag
     9961 atgtattata caaacaacaa agaatagatt ggtcaagtat tatcttaaga aaagatacaa
    10021 tgattttttg atttatatat tccttttatg taaaaaaaat aaaatagttt tgccgaaaaa
    10081 catagagcag aaaatgaatt gcgaacaggc ttttggtatg tattatgcgt ttcgcttgtt
    10141 gttgattaaa aaattctttc cagtttatca tttcttgtat caagtaaaaa acagatagtt
    10201 tcttataaaa aatgttttta aaggagaggg tatggctgtt ctcgtgactg gtggggcagg
    10261 atatattggt tcacatacag tattgcaact tttaaatcat aactatgatg ttattgttat
    10321 tgataatttt agtaactctt caaagattgc tttaaaaaga gtggagtatt tgacttctaa
    10381 gaaaataatt atatatgaag gagatatacg caacattgac ttgattgatt ctgtgtttgc
    10441 aaataatgat atcgaggctg ttattcattt tgctgcgcta aaatctgttt cggaatcaat
    10501 taaaaaaccc attcagtatt atgagaataa tgttattgga actttgcatt taattgactc
    10561 gatgttaaaa aataaaattt acaattttat ttttagttca tccgcaacag tatatggcaa
    10621 tcctacagct attccggtgc acgaaaattg cagtacagga ggaacaacaa atccatatgg
    10681 gacgacaaaa tatattgttg aaagaatgct tgaagatata accaattatg aatttaaatt
    10741 taatgcaact tgcttgagat attttaatcc tgtaggcgca cattattctg gtttaattgg
    10801 tgaggatcct aatggtgtac caagcaacct ttttccctat ttaacacagg ttgctgttgg
    10861 gaaacttgag aagttgacca tatatggggg ggattatcct acggttgatg gcaccggagt
    10921 tagagattac atacatgtta tggacttagc ggaaggtcat attgcagcgc taaaaaaaca
    10981 aaatatatac ggtgggttac gttattataa cctaggcaca ggtaaaggat attcagtaaa
    11041 agagattatt gatgcctttc aaaaaataac cggaataaaa ataccttatg ttatttctga
    11101 gcgaaggctt ggagatatcg ctgaatgttg gtcaaaacca gatttagcta ttaaagagct
    11161 tcattgggaa gcaaagcgta cacttgagga tatgattact gatgcttgga gatggcaaaa
    11221 taaaaatccg aatggatatt ctgagtgata ctgagtcagt atttatcata atattatcta
    11281 ctatcatcaa atgatatcag ttaatataac gctaactcta aaagtattta gttttccacc
    11341 tgacaggagt aaacaatgtc aaagcaacag atcggtgtcg tcggtatggc agtgatgggg
    11401 cgcaaccttg cgctcaatat cgaaagccgt ggttataccg tctctatttt caaccgttcc
    11461 cgtgaaaaga ccgaagaagt gattgccgaa aatccaggca agaaactggt tccttactat
    11521 acggtgaaag agtttgttga atctctggaa acgcctcgtc gcatcctgtt aatggtgaaa
    11581 gcaggtgcag gcacggatgc tgctattgat tccctcaagc catacctcga taaaggtgac
    11641 atcatcattg atggtggtaa taccttcttc caggacacca ttcgtcgtaa ccgtgagctt
    11701 tctgccgaag gctttaactt cattggtacc ggtgtctccg gtggtgaaga aggcgcgctg
    11761 aaaggtcctt ccattatgcc tggtgggcag aaagaagcct atgaactggt tgcgccgatc
    11821 ctgaccaaaa tcgccgcagt agctgaagac ggtgagccat gcgttaccta tatcggtgcc
    11881 gatggtgcag gtcactatgt gaagatggtt cacaacggta ttgaatacgg cgatatgcag
    11941 ctgattgctg aagcctattc tctgcttaaa ggtggcctga atctttccaa cgaagaactg
    12001 gcgcagacgt ttaccgagtg gaataacggt gaactgagca gctacctgat cgacatcacc
    12061 aaagatatct tcaccaaaaa agatgaagac ggtaactacc tggttgatgt gattctggat
    12121 gaagcagcaa acaaaggtac gggcaaatgg accagccaga gtgcgcttga tctcggcgaa
    12181 ccgctgtcgc tgattaccga gtctgtgttt gcacgttata tctcttctct gaaagatcag
    12241 cgtgttgccg catctaaagt tctctctggc ccgcaagcac agccagcagg cgataaggct
    12301 gagtttatcg aaaaagttcg tcgtgcgctt tatctgggca aaatcgtttc ttacgctcag
    12361 ggcttctctc agttgcgtgc tgcgtctgaa gaatacaact gggatctgaa ctacggcgaa
    12421 atcgcgaaga ttttccgtgc tggctgcatc atccgtgcgc agttcctgca gaaaatcacc
    12481 gatgcatacg ccgaaaatcc gcagatcgct aacctgctgc tggctccgta ctttaagcaa
    12541 atcgccgatg actatcagca ggcgctgcgt gatgtcgttg cttatgcagt gcagaacggt
    12601 atcccggttc cgacattcgc cgctgcggtt gcctattatg acagctaccg cgccgctgtt
    12661 ttgcctgcga atctgatcca ggcacagcgt gactatttcg gtgcgcatac ttataagcgc
    12721 attgataaag aaggtgtgtt ccataccgaa tggctggatt aatctgattt aaatcaatta
    12781 atcaatgcaa ggcccggaga aaccctccgg gcttttttat tatacaaagc ggcaggttat
    12841 ggcctttttt tataatttat ggttaaaaaa gcgatataat acagagccgc acagcaggat
    12901 cgctgccttg acagttcatc tacatcagcg ttaaaaatcc cgcagtagat gaagctgtgg
    12961 tggtggatta atgaccactc taaatgttta accggaagaa gtcagagcta atgaaaataa
    13021 caatttcagg aacaggttat gttggtcttt caaatggtat tctgattgcg caaaaccacg
    13081 aagtggttgc actggatatc gttcaggcca aagtggacat gcttaacaag aagcagtcac
    13141 cgattgttga taaggagatt gaagagtatc tggcgactaa agatctcaat ttccgcgcta
    13201 cgacagataa gtatgacgcg tataaaaatg ccgattacgt tattattgcc acgcctaccg
    13261 attatgaccc aaaaacaaat tattttaata cctcaagcgt ggaagcggtc attcgcgatg
    13321 tgacagaaat taatcccaac gcggtaatgg tgattaaatc tacaattccg gtaggtttca
    13381 ccaaatcaat taaagaacgt ctgggtatcg ataaccttat tttctccccc gaattcctcc
    13441 gcgaaggtaa agccctttac gataaccttc atccgtcacg tattgtcatc ggtgagcgtt
    13501 cggaacgcgc agaacgtttt gcggcgttat tacaggaagg cgcgattaag caaaatatcc
    13561 caaccctgtt taccgactcc actgaagcag aagcgattaa acttttcgct aatacctatc
    13621 tggcgatgcg cgtagcatac tttaatgaac tggatagcta tgcagaaagt ttagggctga
    13681 atactcgcca gattatcgaa ggcgtttgtc tcgatccgcg tattggcaac cattacaaca
    13741 atccgtcgtt tggctatggt ggttattgtc taccgaaaga taccaagcag ttactggcga
    13801 actaccagtc tgtgccgaat aacctgatct cggcaattgt cgatgcaaac cgcacgcgta
    13861 aagattttat tgccgatgcc attttgtcac gcaaaccgca agtggtgggt atttatcgcc
    13921 tgattatgaa gagcggttca gataacttcc gcgcgtcttc cattcagggg attatgaaac
    13981 gtattaaggc taaaggcgtt gaggtgatca tctacgagcc ggtgatgaaa gaagactcat
    14041 tcttcaactc tcgtctggaa cgtgatctcg ccaccttcaa acaacaagcc gacgtcatta
    14101 tttccaaccg tatggcagaa gagcttaagg atgtggcaga taaggtctac acccgcgatc
    14161 tctttggcag tgactaacat cctgttatca gggcgatttt cgccctgatt ctcttatgtt
    14221 ccctttgtaa taattcattc tttttatcat ttatcctata gcattcatgg tgattatcgc
    14281 taaactatgg cgacttaaaa ttatttcgtc cgttagggta atgatgagag tagaaaataa
    14341 taatgtttct gggcaaaacc atgacccgga acagattgat ttgattgatt tactggtgca
    14401 gttgtggcgt ggcaagatga caattatcat ctctgtcatt gtggctattg ccctggctat
    14461 agggtatctg gccgttgcca aagaaaagtg gacttccaca gctatcgtta ctcaacctga
    14521 tgtggggcaa atcgctggtt ataccaacgc gatgaatgtg atctacggat cggctgtacc
    14581 gaaagtctcc gacattcagg cttcacttat cggacgttac agcactgcat tttcagcatt
    14641 agcggaaacg ctggataatc aggaagaagc agaaaagctg accattgagc ctaccgttaa
    14701 aaatcagtca ttacctttag cggtgtctta tgttggtgaa acacctgaag gtgctcagaa
    14761 gcagctggcg aagtatatcc agcaagttga cgaccaggta aacgaagaac tggaaaaaga
    14821 cctgaaagac aacatcgcgc tgcgtatgaa aaacttgcag gattctctga aaactcagga
    14881 agtagtcgcg caggagcaga aagatctgcg tatccgtcag attcaggaag cgttgcagta
    14941 tgcgaatcag gcgcaggtga caaagccaca ggttcagcag actgaagatg tgacacaaga
    15001 tacgttgttc cttttgggga gcgaagcgct ggagtcgatg attaagcacg aggcgacccg
    15061 tccgttggta ttctcaccaa actactatca gacacgtcaa aacctgctgg atatcgaaag
    15121 cttaaaggtt gatgatcttg atattcatgc ttaccgttat gtgatgaaac cgacgttacc
    15181 tattcgtcgc gatagcccga aaaaggcaat taccttgatt ctggcagtgc tgctgggcgg
    15241 catggttggc gcggggattg tgctggggcg taacgctctg cgtaattaca acgcgaagta
    15301 atcttttggt tttaaagaaa acgggcaggg tggtgacacc ttgcccgttt tttttgccgg
    15361 atgcgacaac aacatcgcat ctgatcaccc gcaactcact gatgccgttt acgcaggttc
    15421 tcaattaccg tcgttaaatc cagatcctga tcctgcaaca ataccagcag gtgatacatc
    15481 aagtcagatg cctcgttggt cagctcaaag cggtcatgta ccgttgctgc cagtgcggtt
    15541 tccacgcctt cttcgcccac tttctgcgca atgcgcttgg tgccgctggc atacagtttg
    15601 gcggtatagg aggtttccgg gtcggcagat ttgcgctcgg cgagcagttg ttccagttga
    15661 tacaggaaca gccactggtg agcggtgtcg ccgaagcagc tgctggtgcc tttgtggcag
    15721 gttggcccga tgggattcgc cagtaccagt aacgtgtcgt tgtcgcagtc cggggcaata
    15781 ctcaccacat tgaggaaatt gcccgacgtt tcgcctttgg tccacagtcg ctgtttagtg
    15841 cgcgagaaga aggtgacttt gccgctttcg agggttttgc ctaaggcttc cgggttcata
    15901 tagcccagca ttagcacttc gccagatacc gcgtgttgca caatcaccgg catcagtccg
    15961 tcggtttttt cccagtccag ttcgcgacgt tgttgttctg ttaacatatc ctgatctcca
    16021 cgccctgtgt tgccaggtac gcttttaatt caccaatatt gattatttgt ttgtggaata
    16081 cggaagctgc cagcgcaccg tcaacgtcgg catcgcggaa agcttcgagg aagtgttcca
    16141 tggtgcccgc gccaccggaa gcaatcagcg ggatgtggca aacttcacgc acttttttca
    16201 gctgctctag gtcgtaaccg ttacgcacgc cgtcctgatt catcatgttg aggacgattt
    16261 ctccggcacc gcgtttttgc acttcctgca cccagtcgag tgtttcccac tgagtgacgc
    16321 gggtgcggct t
//
