LOCUS       AB812082               22931 bp    DNA     linear   BCT 13-DEC-2014
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: 182-02.
ACCESSION   AB812082
VERSION     AB812082.1  GI:733167551
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. (2014) In press
   PUBMED   25428893
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22931)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..22931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="182-02"
                     /db_xref="taxon:562"
                     /note="serogroup:O186"
     gene            <1..906
                     /gene="wcaM"
     CDS             <1..906
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ02057.1"
                     /db_xref="GI:733167552"
                     /translation="NYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILI
                     SDHLIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVE
                     NGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVI
                     KGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNK
                     PQHLFMRNIKVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSS
                     VDIDRINHHIVNVEKINFRLPERRE"
     gene            1069..1974
                     /gene="galF"
     CDS             1069..1974
                     /gene="galF"
                     /note="O186_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ02058.1"
                     /db_xref="GI:733167553"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLNE"
     gene            2347..3420
                     /gene="rmlB"
     CDS             2347..3420
                     /gene="rmlB"
                     /note="O186_02"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAQ02059.1"
                     /db_xref="GI:733167554"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNSNEALPLFTEMTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVMTQGVVGETYNIGGRNEKKNLDVVNTICDL
                     LDEIVPKQGSYRDQIIYVTDRPGHDRRYAIDASKISDELGWKPQETFESGIRKTIGWY
                     LNNLEWCRRVQDGSYQRERLGLQ"
     gene            3421..4290
                     /gene="rmlA"
     CDS             3421..4290
                     /gene="rmlA"
                     /note="O186_03"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAQ02060.1"
                     /db_xref="GI:733167555"
                     /translation="MKGIILAGGSGTRLYPVTMAVSKQLLPVYDKPMIYYPLSTLMLA
                     GIRNILIISTPQDIPRFMGLLGDGSQWGLQLEYKIQNSPDGLAQAFILGEDFIGNDNC
                     ALILGDNIFYGHDLQKHLEIALSKDKGATVFAYHVKDPMRYGVVEFDKQGKAISLEEK
                     PEIPKSNYAVTGLYFYDNNVVEIAKSLKPSKRGELEITDVNRLYLERGELSVAMMGRG
                     YAWLDTGTHESLIEASNFIQTIEARQGLKVSCPEEIAFNKKFIDKTQLIKLAKPLEKN
                     SYGKYLIKLAESN"
     gene            4390..5853
                     /gene="wzx"
     CDS             4390..5853
                     /gene="wzx"
                     /note="O186_04"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ02061.1"
                     /db_xref="GI:733167556"
                     /translation="MLLRRYMNSNVKKNISAVNGLKWSAIERICSQGIQLLLMIVLAR
                     QLGPGAFGLIGMLTIFITIGQVFIDSGFSAALIRKNERTESDYATVFYFNMTVAILFY
                     AVLFFCAPFIAEFYKRNELIELTRVLGLTIIISAFIIVQRIQLSVILDFKTQAISSLS
                     SVIISGGCALLMAYNGFGVWSLVIQTITMGLVNLVILNIYNPWLPKRSFSKKSFHGFF
                     SFGSRLLISSLIDSIYTNIYLVVIGKSFSASTLGQFTQANLLSNTPAMTLTTVVQRVT
                     YPLLSNVNNAKGNIDEIYLRILRLTAAAVFPVMFLLAIIAKPFVVLFLGQQWEPVAEL
                     MSILCIGYCLYPVHAINLNLLQVKGRTDLFLKLEIIKKTLITVILIVTIPFGVKIICI
                     GIFAQYYISLLINTYYTGKLSSLSAIAQIKALLPIWLMASISSAISWFLIPREIFSGL
                     YQIIGILITNISLYGIGMYLFQKDIYEMVKFLFIKTK"
     gene            5858..6988
                     /gene="vioA"
     CDS             5858..6988
                     /gene="vioA"
                     /note="O186_05"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-xylo-4-hexulose aminotransferase"
                     /protein_id="BAQ02062.1"
                     /db_xref="GI:733167557"
                     /translation="MNKEMKMLNGKILVTQPFLPELREFIPYLEKIWENKWLTNNGPF
                     HQQLENDLCRYLGVEYVSLFNNATIALITAVQSLELTGEVITTPYSFVATTHSLMWNN
                     LNPVFVDVSRDTFNINPSQIEAAITEKTTAIMAVHCYGNPCDVIAIEKIAKKYKLKVI
                     YDAAHAFGVNFKGESLLKYGDLSVVSFHATKVFNTFEGGVIICPNAETKLKIDQLKNF
                     GFEDELTIKSIGINGKMSEVNAAFGLVQLKHVNEAISKRKEINDLYGKLLGNVKGISL
                     AKFDKLATKNFSYYPILIEDDYGMSRDELCHLLQKNNIFARKYFYPLISDMDLYKNME
                     SARKENLHIARDISNKVLCLPIYADLDLDIVRFIARVIGNKK"
     CDS             6985..7692
                     /note="O186_06"
                     /gene="wbaN"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ02063.1"
                     /db_xref="GI:733167558"
                     /translation="MKLAIMQPYLFPYLGYYQLMSSVDKFIIYDDVSYIKNGWINRNR
                     ILVNGNAHYFTVPVIGGSCNNKINTVKIDKTKKKAINKIIITIEQAYKKSVFFDEVFP
                     VIYGVLSKEYDFISDLAITSLLSIKNKLDIGAEVVLTSTNYGNNNLTSQDRVIDINVK
                     EHASTYINSEGGRLLYDKKTFKLNGVNLKFIHPEILPYKQLCNGEFVPSLSIIDVVMN
                     NGWDTTKQLVNSFELKD"
     CDS             7696..8670
                     /note="O186_07"
                     /gene="wbaO"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ02064.1"
                     /db_xref="GI:733167559"
                     /translation="MREHNYAIGGYFSLELQALKNNTANEKVYLQSARACFQLLLESI
                     EVSRVWLPYYICDVVVDTINEIGIEILYYSISKDFIPQTFPVLEENDVFVYVNYFGVC
                     DEQTKLILQKYPPEKVILDNSQAFYSGHNNNLGTIYSPRKFFGVPDGGILITNQTIIL
                     PSSQDNDSSQYINHLIGRLISHPSEYYTDYIKAEERLKKIKKAKVMSYLTRKLLDSIN
                     YHEIKKIRDDNFKFLHHALEKINGIKIPEIVNGPLCYPLLSKNNKLKDILIKNEIYVP
                     TYWKDVLNRVDINSTEFEFVSNLIPLPCDQRYSSIQMKKIINIVLEEN"
     CDS             8670..9236
                     /note="O186_08"
                     /gene="wbaP"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="BAQ02065.1"
                     /db_xref="GI:733167560"
                     /translation="MNIIGKTVKLRAVEIDDLELLNKWANDPEIWYMLGGWHFPYSKN
                     NTEKWIKNIDNNDSKNQIFAIETEEHGLIGTANLVNIDWKNKNAFHGIMLGNVETRGK
                     GYAQDVVMSLMRYAFDELGLNRLDGDMIEYNKLSINFYIKRCGWKIEGIKKEWFFRKG
                     QYFDKVVVGITKKEYLEHIEKSKYWETK"
     CDS             9236..9634
                     /note="O186_09"
                     /gene="wbaQ"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ02066.1"
                     /db_xref="GI:733167561"
                     /translation="MERFKLGDTATYTQTITDADIKSFAGISGDNNPVHMSDEYAEGS
                     RFKKRIAHGLISASFFSALFGTKLPGPGCVYVNQSLKFLRPVYINDTVTARVVLTDID
                     VVKRRLFFDTICEVNRKKVITGKAEIYLPE"
     CDS             9708..10733
                     /note="O186_10"
                     /gene="wfbE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ02067.1"
                     /db_xref="GI:733167562"
                     /translation="MNVSMSDIKVSVCIISFNQQNYIRQCLDGVFSQKTNFEYEVIIR
                     DDCSTDNTYLTIMEYIDTLDEEKKKNIKITVLDGTKNIGANNNFIETFKTSVGQWLAI
                     CEGDDYWCDQGKLQKQYDYAISHSDCSLVVHPALISENNVIRKTSWACMNKTINQLSD
                     VIRAKGQFSPTGSYFFKREILNVLPLWFSTAPVGDYYMEIFATSLGSCHTIPDAMSVY
                     RINSTGSWSDLLKKDRNGQRIINTYLSQLEYLDKLAEIFPSCVDDITIKRSHAQYAAA
                     MGYLANGDYTNFRILMDKSDTNGWYDNMHSIFYYLRNSRALSMLMFRFKPAIKSAVMW
                     GRRVFNN"
     gene            10717..11967
                     /gene="wzy"
     CDS             10717..11967
                     /gene="wzy"
                     /note="O186_11"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ02068.1"
                     /db_xref="GI:733167563"
                     /translation="MYLTTNYCDFLSRTRIVRRIFLLVYLISNVYAYIVFTETGVLIG
                     DYQGVLIIYKDQVVYLLMLTIMSYYIVCGPVFNCLSKVKIRLRTFKGLNSFAYILFFF
                     QILFAVFNISTGSNAAGTDFQTGGVIRLLWLFLPVDYLFYIYYFVGREKKVSKIYLAN
                     VVIFILSMLSRGWLGWTLVLLYAELCFFFYSQKKIKIKYLILLFFLLIVAPLAFSLKI
                     QLRADLYSSGIGGVISTLSNIDYIQSYNNFIAGFLSRIQQLSNIVFFYDHQQELYKFV
                     SSDIVSNYAWEGLPQQTVAKLLGLDPGVDMHIFLYSHYISSTSEAVTTLQVGFISWLF
                     LGTLSSVFYPLFVFAIICISLFLSKKLGGEKLCALTWIMIFLSIMCGWYNAYLVYMQA
                     LITFYFIMGFLNLITLEKTKINHT"
     CDS             11964..13085
                     /note="O186_12"
                     /gene="wbwG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ02069.1"
                     /db_xref="GI:733167564"
                     /translation="MMDDEKYMSNLVIVNATALASSGALTILNQFLEHALNDSKHKYL
                     CFIHESVKKKSVNNVTVITIKKQSFIQRVWWDLYGLNKYIKKNNLKPKKIVSLQNTSV
                     NSNFEQIIYLHQPIPFSNFRIKIKFQYAIFFMYKYVYPFFIFFRTKNTTFVVQTDWMK
                     DAIVAKKKIAKERVHVIKPDIILPVNNLAYPDEHDKDNSSEVFFLYPATPLFYKNHLI
                     ILDAMRILKTEGILANTKFQVTFKQDDNDELAIKIAKYDLVDNISFLGVLSYEELFHK
                     YLKADAILFPSYLESFGLPLAEGAMLGKYIICSDLPYARDVLNNYSNVEYINHDDASK
                     WALAMKEIILKNRNNTLSESENSYMYCPKTSWADFFELI"
     gene            13086..14129
                     /gene="fnlA"
     CDS             13086..14129
                     /gene="fnlA"
                     /note="O186_13"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase"
                     /protein_id="BAQ02070.1"
                     /db_xref="GI:733167565"
                     /translation="MRYTMFKNKVLLITGGTGSFGNAVLQRFLDSDIGEIRIFSRDEK
                     KQDDMRKKYASDKLKFYIGDVRDYSSVLTATRGVDFIYHAAALKQVPSCEFYPMEAVK
                     TNVIGTDNVLEAAIANKVSRIVCLSTDKAVYPINAMGTSKAMMEKVIVAKSRNLPKDI
                     TICATRYGNVMASRGSVIPLFINQILEGRPITITDPCMTRFMMTLDDAVDLVLHAFEH
                     GTNGDIFVQKAPAATIDTLTKSLLKLTKQHSHPINIIGTRHGEKLFEVLCSREEMLVA
                     EDQGNYYRIPSDKRDLNYEKFFDKGTKEIQFVEDYNSHNTRRLDVDEMVALLRKLDYI
                     NKIEAGEKADPDA"
     gene            14095..14991
                     /gene="qnlA"
     CDS             14095..14991
                     /gene="qnlA"
                     /note="O186_14"
                     /codon_start=1
                     /transl_table=11
                     /product="L-QuiNAc synthase"
                     /protein_id="BAQ02071.1"
                     /db_xref="GI:733167566"
                     /translation="MRLVKRQIPMRKKILIVGANGMLGSSLLRYFSSIGDYEVLGTTR
                     SMVVAKQLEQKHNVKIIDNVDVIDFKRLETVVVEHKPNIVFNCVGIIKQLDAAKNNIL
                     SIEINSLLPHKLAQLCSAHSAKLIHFSTDCIFKGTKGNYVEDDESDAIDLYGKSKFLG
                     EVEYNGHLTLRTSIIGHELGSNHSLVDWFLSQKKSVKGFTNAIFSGLPTCYMAEVIHK
                     YVLPNNLAGLFHLSVEPISKYDLLNIIKIVYGVSTDIEPTNEFKIDRSLNSTLFRNKT
                     NFVPESWDKLIEKMKDEYNKYF"
     gene            14969..16123
                     /gene="qnlB"
     CDS             14969..16123
                     /gene="qnlB"
                     /note="O186_15"
                     /codon_start=1
                     /transl_table=11
                     /product="L-QuiNAc synthase"
                     /protein_id="BAQ02072.1"
                     /db_xref="GI:733167567"
                     /translation="MNTINISNSKIRVVTVVGTRPEIIRLSRVIAVLDEYTEHFLVHT
                     GQNYDYELNEVFFNELEIRKPDFFMNAAGQNAAETIGNVIIEADKIFDKLQPEALLIL
                     GDTNSALVSIAAKRRKIPIFHMEAGNRCFDYRVPEEINRKIVDHISDINLTYSEIARD
                     YLLREGLPADQIIKTGSPMREVLNFYKDKISSSSILEKLNLQASSYFLVSSHREENVD
                     SPEKLRSLIETLNIVSEKYKLPVIVSTHPRTRNRIDALGITVSNNIIFSKPFGFLDYI
                     KLQQNARVVLSDSGTITEESSVLNFPALNLREVHERPEGFEEAVVMFVGLDRNRIIQG
                     IDILQGQKRGDNDRDLHMVTDYQADNVSIKILRIIMSYTNFINQKVWKKF"
     CDS             16108..17316
                     /note="O186_16"
                     /gene="wbwH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ02073.1"
                     /db_xref="GI:733167568"
                     /translation="MEKILMRIALICDDYLPDSTRVSAKMMHELACELLEKGHEPIVI
                     CPCNKIQTLEILNLDGVVVYKFPNGAIKNVSKISRAINESMLSFNAWRFIGKYIRETK
                     IDGVVYYSPSIFFGKLANKIKENWHCKSYLILRDSFPQWLVDQGIIKEGGLAERYFRY
                     FEQINYDAADYIGLMSDRNKDIFINKYQNKYKVQTLFNWADFKGIDNIPSATLRSKLA
                     LQNKVIFFYGGNIGHAQDMMNLMRLVRSASYRDDVHFLLIGQGDEVSLVKQFIIDNSL
                     KNCTYLPSITQSEFKSVLKIVDVGLFSLAKNHTVHNFPGKLLGYMANKLPILGSVNAS
                     NDVMEIINGAKAGFVFVNGNDEALLNAAINLADDTQLRKNLGCNAYSLLQEKFSVEMA
                     AEKILSSLFS"
     CDS             17335..17832
                     /note="O186_17"
                     /gene="wbaR"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ02074.1"
                     /db_xref="GI:733167569"
                     /translation="MKVSFVSQDDFENLIQEAFASVRLRSHLLLHESPNDAVQRIMIG
                     LVKGTYIPPHFHEFQHQWEHFHVFQGEVELMLFDSNGCLNKKVILGGQSKNIIAQISP
                     LTPHTLVCRSPTAVIMEIKEGPFDEKCAKVIPSWSYSEDYSILSRDRIIAMMGQLSIG
                     DRFSL"
     gene            17984..19390
                     /gene="gnd"
     CDS             17984..19390
                     /gene="gnd"
                     /note="O186_18"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ02075.1"
                     /db_xref="GI:733167570"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            19639..20805
                     /gene="ugd"
     CDS             19639..20805
                     /gene="ugd"
                     /note="O186_19"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ02076.1"
                     /db_xref="GI:733167571"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQHFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            20895..21929
                     /gene="wzz"
     CDS             20895..21929
                     /gene="wzz"
                     /note="O186_20"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ02077.1"
                     /db_xref="GI:733167572"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQTSLIGRYSTAFSALAETLDNQEEAEKLTIEPTVKNQPLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIESLKVDDLDIHAYRYVMKPTLPVRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(22026..22637)
                     /gene="hisI"
     CDS             complement(22026..22637)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ02078.1"
                     /db_xref="GI:733167573"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPKALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     7199 a   3922 c   4730 g   7080 t
ORIGIN
        1 aattacgcca tcttacgcca gggatttcat aaccagatta tcggtgccaa catcaccaat
       61 tgtaagttca gcgacttaca aggcgacgcc attgaatgga acgtggcaat taacgacagt
      121 gatattttga tatctgacca tctcatcgag cgcatcaact gtactaacgg aaaaatcaac
      181 tggggcattg gcataggcct tgcggggagt acttatgata ataactatcc ggaagaccag
      241 gcagtgaaaa actttgtcgt ggcaaatatc acgggatcgg attgtcggca gttgatccat
      301 gttgaaaatg gtaaacattt tgttattcgt aatatcaaag cccgcaatat cacgccggat
      361 ttcagtaaga aagcaggtat tgataacgcg acggtcgcta tttacggttg tgacaatttc
      421 gtgattgata atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt
      481 aaaggcaaat atctctcgat accgcagaat ttccgggtga ataatattca actggataac
      541 acccaccttg cttataaatt gcgcggcatc caaatctctg ccgggaatgc tgtctccttt
      601 gtggcgctga ctaacattga gatgaagcgt gcgtcgctgg agttacacaa caaaccgcaa
      661 caccttttta tgcgaaatat caaggtgatg caggaatcct cagttggacc agcattgagt
      721 atgaactttg acatgcgcaa ggacgttcga ggcgtgttta tggcgaaaaa agaaacactg
      781 ctgtctcttg caaatgttca tgcggtgaat gaaaaagggc aaagctccgt cgatatcgac
      841 aggattaatc accatattgt taatgtggaa aagattaact ttagattgcc ggaacggaga
      901 gagtagattt gcgaccattc ctggaaaaat ggagtcatac ttaggaacaa tgctactgca
      961 atccacaacg aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat
     1021 gctggcgaaa aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata
     1081 atgacaaatt taaaagcagt tattcctgta gcgggtctag ggatgcatat gttgcctgcc
     1141 actaaggcga ttcccaaaga gatgctaccg atcgtcgaca agccaatgat tcagtacatt
     1201 gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag
     1261 aacgcggtcg aaaaccactt cgacacctct tatgaattag aatctctcct tgaacagcgc
     1321 gtgaagcgtc aactgctggc ggaagtgcaa tctatctgcc cgccgggcgt gaccattatg
     1381 aacgtgcgtc agggcgaacc tttaggtttg ggccactcca ttttatgtgc acgacctgcc
     1441 attggtgaca atccatttgt cgtggtgctg ccggacgttg tgatcgacga cgccagcgcc
     1501 gacccgctac gttacaacct tgctgccatg attgcacgct tcaacgaaac gggccgtagc
     1561 caggtgctgg caaaacgtat gccgggcgat ctctctgaat actctgtcat ccagaccaaa
     1621 gagccgctgg accgcgaagg taaagtcagc cgcattgttg aatttatcga aaaaccggat
     1681 cagccgcaga cgctggactc agacatcatg gccgttggtc gctatgtgct ttctgccgat
     1741 atttggccag aacttgaacg cactcagcct ggtgcatggg ggcgtattca gctgactgat
     1801 gccattgccg aactggcaaa aaaacagtcc gttgatgcca tgctgatgac aggtgacagc
     1861 tacgactgcg gtaaaaaaat gggctatatg caagcgttcg tgaagtatgg gctgcgcaac
     1921 ctcaaagaag gggcgaagtt ccgtaaaggg attgagaagc tgttaaacga ataatgaaaa
     1981 tctgaccgaa tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcgaaa
     2041 gtaatatgtt gcgaatattc ctgccgttgt tttatataaa caatcagaat aacaacgagt
     2101 tagcaatagg attttagtca aagttttcca ggattttcct tgtttccaga gcggattggt
     2161 aagacaatta gcgtttgaat ttttcgggtt tagcgcgagt gggtaacgct cgccacatcg
     2221 taggcatgca tgcagtgctc tggtagctgt aaagccaggg gcggtagcgt gcattaatac
     2281 ctctattaat caaactaaga gccgctaatt taacagcatg ctctgaagta atatggaata
     2341 aattaagtga aaatacttgt tactggtggc gcaggattta ttggttctgc tgtagttcgt
     2401 cacattataa ataatacgca ggatagtgtt gttaatgtcg ataaattaac gtacgccgga
     2461 aacctggaat cacttgctga tgtttctgat tctgaacgct atgtttttga acatgcggat
     2521 atttgcgatg ctgctgcaat ggcgcggatt tttgctcagc atcagccgga tgcagtgatg
     2581 cacctggctg ctgaaagcca tgtggatcgt tctatcactg gccctgcggc atttattgaa
     2641 accaatattg ttggtactta tgtcctttta gaagccgctc gcaattactg gtctgctctt
     2701 gatagcgaca agaaaaatag tttccgtttt catcatattt ctactgacga agtatatggc
     2761 gacttacccc atcctgatga agtaaatagt aatgaagcgt taccgctatt tacggaaatg
     2821 acagcttatg caccaagtag cccatattct gcttctaaag catccagcga tcatttagtc
     2881 cgcgcgtgga aacgtaccta tggtttaccg accattgtga ctaattgttc taacaattat
     2941 ggtccttatc atttcccgga aaaactgatt ccattggtta ttcttaatgc tctggaaggt
     3001 aaggcattac ctatttatgg taagggggac caaattcgcg actggttgta tgtagaagat
     3061 catgcccggg cattgtacac cgttatgacc cagggtgtag taggtgaaac ctacaatatc
     3121 ggtggtcgta acgagaaaaa aaacctggac gtggtaaata ctatttgcga tctgcttgat
     3181 gagattgtac ctaaacaagg gtcttatcgc gatcaaatta tctacgttac tgaccgtcct
     3241 gggcacgatc gccgctatgc gattgatgca tcaaaaatca gcgatgagct gggctggaaa
     3301 ccgcaggaaa cctttgaatc gggaattcga aaaactattg gttggtattt aaataattta
     3361 gagtggtgcc gtcgtgtgca agacggtagc tatcaacgtg aacgtttagg attacagtaa
     3421 atgaaaggta tcatacttgc aggtggttca ggaactcgtt tatatccagt cacaatggct
     3481 gtaagtaaac aattgttgcc agtttatgat aaaccaatga tttattaccc attaagtaca
     3541 ttaatgttgg ctggtatacg aaatatactt attataagta cacctcaaga tattcctcgt
     3601 tttatggggt tattaggtga tggtagtcaa tggggacttc agttagaata caaaatacag
     3661 aatagtcctg atggattagc gcaagcattt atattaggtg aggattttat tggaaacgat
     3721 aattgtgcat tgattttagg ggataatata ttctatggac atgatctcca gaaacaccta
     3781 gaaatagcgc tctcaaaaga taaaggtgct acagttttcg cttatcatgt taaagaccct
     3841 atgagatacg gcgttgtaga gtttgataaa caaggtaaag ccatttcact tgaagagaaa
     3901 cctgaaattc caaaaagtaa ttatgcagta acgggattgt atttttatga taataatgtt
     3961 gttgagatag cgaaatcatt aaaaccctcg aagcgaggag agctagaaat aacggatgtt
     4021 aatcgactct atttggagcg gggtgagctt tctgtagcta tgatggggcg aggttatgct
     4081 tggctagata ctggaactca tgaaagtcta attgaagcta gcaatttcat ccagacaatt
     4141 gaagctagac agggtttaaa agtatcctgc cctgaagaaa tagcatttaa taaaaaattt
     4201 attgataaaa cgcagttaat aaaattagct aagcctttgg agaaaaattc atacggaaaa
     4261 tatttaataa aactggcaga gtcgaattga ttttttatga atgattaatt tgttcataga
     4321 ataaaaagtg aatgcctcca tggttaatga gttaatttaa caactaagaa ggtgagtatc
     4381 tatctctaag tgttattgag aaggtatatg aattcaaatg taaaaaaaaa tattagcgct
     4441 gtaaatggat tgaagtggag tgcgatcgaa agaatatgtt cacaaggtat ccagctactt
     4501 ttaatgatag tcttggctag acaattaggg cctggtgcat ttggccttat tggaatgctg
     4561 acaatattta ttacaatagg tcaggtcttt attgatagtg gttttagtgc cgctctcatt
     4621 cgaaaaaatg aaagaacaga atcagactat gcaactgttt tttactttaa catgacagtt
     4681 gccattctgt tttatgcagt gttatttttt tgcgccccat tcatagctga attttataag
     4741 cgtaacgaat taattgaatt aacaagagtt ctgggtttaa caataataat aagtgctttt
     4801 attattgttc aacgaataca attaagtgtc attttggatt tcaaaactca agctatatcg
     4861 tcattatcca gtgtcataat ctcaggaggg tgtgcactat taatggcata taatggtttt
     4921 ggtgtatggt cgttagttat acagactatt accatggggc ttgttaattt agttatttta
     4981 aatatatata atccatggtt accgaagagg agtttttcaa aaaaatcatt tcatggattt
     5041 ttttcttttg gctccagact tctgatttca tcactgatag attcaatata cactaatatt
     5101 tatttggtag ttatagggaa gtcctttagc gctagcacac tgggccaatt tacacaagct
     5161 aatttattat caaatacgcc ggccatgacg ttaacgacag ttgtacaaag agttacctat
     5221 ccattattaa gtaatgtgaa taatgctaag gggaatattg acgagatata tcttaggata
     5281 ttaaggctta ctgccgcagc cgtttttcca gtaatgttcc tattggcaat aattgctaaa
     5341 ccttttgtcg ttctatttct tggccaacaa tgggaacctg ttgcggaatt aatgagcata
     5401 ttatgtatag gatactgttt atatccagta catgctatta atctaaactt attacaggta
     5461 aaaggacgaa ctgatttatt tttgaagtta gaaataataa aaaaaactct catcacggtt
     5521 attctaatag taacaatacc atttggtgtt aaaataatat gtataggtat ttttgcacag
     5581 tattatatat ccttgttgat aaatacatac tatacaggga aactcagcag cttaagtgca
     5641 attgcacaga taaaggcatt attgccaatt tggttaatgg catctatcag ttcggcaatt
     5701 agttggttct taataccaag agagatattt tcgggattat atcaaataat aggaatattg
     5761 ataaccaata tttcattata tggaatagga atgtatcttt tccaaaaaga tatttatgaa
     5821 atggtaaagt ttttatttat aaaaacaaaa taattttatg aacaaagaga tgaaaatgtt
     5881 aaatggcaag attttagtaa cgcaaccatt tttacctgaa ctaagagagt ttattcccta
     5941 tctggaaaaa atatgggaaa ataaatggtt aacaaacaat ggtccatttc atcagcaatt
     6001 agaaaatgat ttgtgccgtt atttaggcgt ggagtacgtc tccttattta ataatgctac
     6061 aattgctctg attacggcag tccaatcatt agaattgact ggtgaagtaa ttacaacccc
     6121 gtattcattt gtggcaacaa ctcactcatt gatgtggaat aatctaaatc cagtatttgt
     6181 cgatgtcagt agagatacat ttaatatcaa tccgtctcaa attgaagcgg cgataacaga
     6241 aaaaacaaca gccataatgg cagtccattg ttatggtaat ccttgtgatg tgattgcaat
     6301 agaaaaaata gccaaaaagt ataaacttaa agtgatatat gatgctgctc atgcttttgg
     6361 ggtaaatttt aagggggaaa gtttattaaa atacggagat ttatcggttg ttagttttca
     6421 tgcaactaaa gtatttaata catttgaagg tggcgtgatt atttgcccaa atgcagaaac
     6481 taagctcaaa atagatcagc taaaaaactt tggttttgaa gatgagctaa caataaaatc
     6541 aattggtatt aatggaaaaa tgagtgaggt caatgcagca tttggccttg tacagttgaa
     6601 acatgttaat gaagctataa gtaaaagaaa agaaattaat gatttatatg gcaagttatt
     6661 agggaatgtg aaaggcatat cattagcaaa atttgataaa ctagctacga aaaatttttc
     6721 atactacccg attcttattg aagatgacta tgggatgagt cgagatgaat tatgtcattt
     6781 acttcaaaaa aataatatat ttgctaggaa atatttttat cctttaataa gcgatatgga
     6841 tttatataaa aatatggagt cagcgagaaa agaaaatcta cacattgctc gagatatttc
     6901 caacaaagta ctttgcttac caatttatgc agatcttgat ttagacattg tgagatttat
     6961 agcgagagta ataggtaata aaaaatgaaa ttagcaataa tgcaacccta tctatttcct
     7021 tatctaggtt attatcaatt aatgtcatca gttgataagt ttattattta tgatgatgtt
     7081 tcatacatca aaaatggttg gataaacaga aatagaattc ttgttaatgg taatgctcat
     7141 tattttactg tgccagttat aggtgggagt tgtaataata aaataaatac tgttaaaatt
     7201 gacaagacaa agaaaaaagc tatcaataaa atcattatta caattgaaca agcctataaa
     7261 aaatccgttt tttttgatga ggtctttcca gtcatttatg gtgtgttatc taaagagtat
     7321 gatttcatat ccgatctggc aataacgtca ttattgtcaa taaaaaacaa acttgatatt
     7381 ggagcagaag ttgttttgac ttcgaccaac tatggtaaca ataatttaac ttcgcaagat
     7441 cgcgtcattg atattaatgt aaaagagcat gcgtccactt atattaattc tgaaggcggg
     7501 agattacttt atgataaaaa gacattcaaa ctaaatggcg taaatttgaa atttatacat
     7561 ccagaaattt taccttacaa acaactttgt aatggtgagt ttgtaccatc cttatcaatt
     7621 atagatgtag taatgaataa tggttgggat actacaaagc agttagtaaa tagctttgaa
     7681 ctgaaggatt gaacaatgcg tgagcataat tatgcgatcg gaggatattt ttcactagaa
     7741 ttgcaggcgc ttaaaaataa cactgcaaat gaaaaagtat atttacaatc agcgagggca
     7801 tgttttcaat tactgctaga aagcattgaa gttagtagag tatggttacc atattatata
     7861 tgtgatgtcg tcgttgatac aataaatgaa attgggattg aaatcttgta ttatagtatt
     7921 tctaaagatt ttattccgca gacgtttcca gttttagaag aaaatgatgt ttttgtctat
     7981 gttaattatt ttggggtgtg tgatgagcaa acaaagttaa ttttgcaaaa atatcctcca
     8041 gagaaagtta tcttagataa ctcacaagct ttttatagcg gacacaataa taatttagga
     8101 acaatttatt cacctaggaa attttttggc gtccctgatg gtggtattct gataactaat
     8161 cagactataa tattaccttc ttcacaggat aatgattcat cacagtatat aaatcatctc
     8221 attgggcgtc taatatccca tcccagcgaa tactatactg attatattaa agctgaagaa
     8281 cgattaaaaa aaattaaaaa agccaaagtg atgtcttatt taactcggaa attattagac
     8341 tccatcaatt atcatgagat taaaaaaata agggatgata attttaaatt ccttcaccat
     8401 gccctcgaaa agataaatgg aataaaaatt ccggagatag tcaatggtcc attatgctat
     8461 ccattgcttt caaaaaacaa caaactgaaa gatattttaa tcaagaatga aatttatgtg
     8521 cctacatatt ggaaagatgt attgaatcga gtagatataa actcgacaga atttgaattt
     8581 gtctcaaact taatacctct gccatgtgat caacgttact catctatcca gatgaagaaa
     8641 ataatcaata ttgtacttga ggaaaactaa tgaatatcat aggaaaaaca gttaagttgc
     8701 gtgctgtgga aattgatgat ttagaattgt taaataaatg ggctaacgat ccggaaattt
     8761 ggtatatgct tggtggttgg catttcccct attccaaaaa taatactgaa aaatggataa
     8821 aaaatattga taataatgat tcgaaaaatc aaatattcgc tattgaaact gaagagcatg
     8881 gattgattgg cactgctaac ttagtaaata ttgattggaa aaataaaaat gcatttcatg
     8941 ggataatgtt gggaaatgtt gaaacacgag gtaaagggta cgcgcaagac gttgtaatga
     9001 gtctgatgag atatgccttt gatgaattag gattaaatcg acttgatggt gatatgattg
     9061 aatataataa attatcaatt aatttttaca tcaaaagatg tggttggaaa atcgagggaa
     9121 ttaagaaaga gtggttcttt agaaaggggc aatattttga taaagtagtt gttggaataa
     9181 ccaaaaaaga atatttagaa catattgaga aaagcaagta ttgggagaca aaatgatgga
     9241 acgttttaaa ttgggggaca cagcaactta tactcaaact ataactgatg cagatattaa
     9301 aagcttcgca ggtatatctg gggataataa tccagtacat atgagtgacg aatatgcaga
     9361 aggttcaagg tttaaaaagc gtatagctca tggtctgata tctgctagtt ttttttcagc
     9421 tctatttggt acaaaattac ctgggcctgg atgtgtatat gtaaatcaaa gcttgaaatt
     9481 tcttagacct gtttatatta atgatacggt tacagcgcgt gtagtcttaa cagacattga
     9541 tgttgtaaaa agaagactct tttttgatac gatttgtgaa gttaaccgaa aaaaagttat
     9601 cacggggaag gctgaaattt acttgccaga ataaagcgct ttaaatttga tgttaacttg
     9661 ctacatattg atgttttatc tctttttatc tttgaagttt atcaaaaatg aatgtttcca
     9721 tgtcagatat aaaagttagt gtatgtatta tttcttttaa ccaacaaaac tatatacgac
     9781 agtgcttgga tggtgtcttt tctcagaaaa caaattttga gtatgaggtt attatacgtg
     9841 atgattgcag taccgataat acatatttaa caataatgga atatattgac actttagatg
     9901 aagagaaaaa aaagaacata aaaataacgg tacttgatgg cacgaaaaat attggagcaa
     9961 ataataattt tatcgaaaca tttaagactt cagtaggtca atggctagct atatgtgagg
    10021 gagatgatta ttggtgtgat caggggaaat tacaaaaaca gtacgactac gctatttcac
    10081 atagtgattg ctcattagtc gtgcatcccg ctctgataag tgaaaataat gtaatacgta
    10141 aaacatcttg ggcgtgtatg aataaaacaa taaatcagct tagcgatgta atacgtgcaa
    10201 aaggacagtt ctcaccaact ggctcctatt ttttcaaacg agaaatctta aatgttctac
    10261 cattgtggtt ttcaacagct cctgttggtg actattacat ggaaatcttt gctacatcgc
    10321 ttggatcttg tcatacaatt cctgatgcta tgtcagttta tagaatcaat tcaacggggt
    10381 cttggtctga cctgttaaaa aaagatagaa atggccaacg gattattaat acttatcttt
    10441 ctcaattgga gtatttggat aaacttgccg aaatctttcc ttcttgtgtc gatgatatca
    10501 caataaaaag atcgcatgca caatatgcag cagcaatggg ttatcttgca aatggtgatt
    10561 atactaattt tcgtatatta atggataagt cagatacaaa cggctggtat gataatatgc
    10621 attctatttt ttattattta agaaatagca gggcgctttc catgttaatg ttcagattca
    10681 agcctgcaat taaatctgct gtcatgtggg ggcgtcgtgt atttaacaac taattattgc
    10741 gactttttgt cccgaactcg aatcgttagg cgcatatttt tgttagttta tttgatttca
    10801 aatgtttacg cctatatagt gtttactgaa acaggagtat taatagggga ttatcaagga
    10861 gtactcataa tatataaaga tcaggttgtg tatttattga tgttaacaat aatgtcatat
    10921 tatattgttt gtggtcctgt ttttaactgt ctatcaaaag taaaaattag actaaggaca
    10981 tttaaaggct taaactcttt tgcttatatc ctgttttttt ttcaaatcct atttgctgtt
    11041 tttaatattt caacaggttc gaatgccgcg ggcactgatt ttcaaactgg tggagtcata
    11101 agactattat ggcttttttt accagttgac tatctatttt atatatatta tttcgtaggc
    11161 cgagagaaaa aagtaagtaa aatatatctg gccaatgtgg ttatttttat tctatcaatg
    11221 ttaagtagag gatggcttgg gtggactttg gttttgcttt atgcagagtt atgtttcttt
    11281 ttttattcac aaaaaaaaat taaaataaaa tatcttatct tattgttttt cttgcttatt
    11341 gttgcaccat tagcatttag tctaaaaatt caattgcgtg ctgatttgta ttccagtggt
    11401 attggtgggg ttatatcaac tttaagtaat attgattaca ttcaatctta taataatttc
    11461 atcgctggtt tcttatccag aatacaacag ttatcaaata ttgttttctt ttatgaccat
    11521 caacaagaat tatataaatt tgtttcttca gatattgtat caaactatgc ttgggaagga
    11581 ttacctcaac aaactgtagc taaacttcta ggattagatc ctggtgttga tatgcatatt
    11641 tttctttata gtcattatat ttcttcaact tcagaggctg taactacatt gcaagttgga
    11701 ttcatttctt ggctcttttt aggcaccctg tcatccgtat tttatccact gtttgtcttt
    11761 gctattatat gcatatcctt atttttatct aaaaaacttg gaggggaaaa actatgtgca
    11821 cttacgtgga ttatgatttt tctttctatt atgtgcgggt ggtataatgc ttatttggta
    11881 tatatgcagg cattaattac attttatttt atcatgggtt ttttgaattt aattactcta
    11941 gaaaaaacaa aaattaatca tacatgatgg atgacgagaa atatatgagt aatttagtaa
    12001 tagttaacgc aacagcttta gcgtctagcg gcgctttgac gattttgaac cagtttttag
    12061 aacacgcact aaatgattct aagcataagt atctttgttt tatacatgag agtgttaaaa
    12121 aaaagagtgt taataatgtc accgttatta ctataaaaaa acaaagtttt attcaacgag
    12181 tatggtggga tctatatgga ttgaataaat atataaagaa aaataatttg aaacctaaaa
    12241 aaatagtttc cttgcagaat acatctgtaa atagtaattt cgagcagata atatatctac
    12301 accagccgat tccctttagc aattttagaa taaaaattaa atttcaatat gctatatttt
    12361 tcatgtataa atacgtgtat ccttttttta tattctttag aactaaaaac accactttcg
    12421 ttgttcagac cgattggatg aaagatgcta tcgtagcaaa gaaaaaaatt gccaaagaac
    12481 gagttcatgt aattaaacct gatataatat taccggttaa taatcttgct taccctgatg
    12541 aacatgacaa agataattca tctgaagtgt tttttttata tccggccacg ccacttttct
    12601 ataaaaacca tttgattatc ttagatgcaa tgaggattct aaaaacagaa ggaatacttg
    12661 ctaatacaaa attccaagtg acttttaaac aagatgataa tgatgagtta gcaattaaaa
    12721 tagccaaata tgatttagtt gataatataa gcttcttggg agtattatcc tatgaagaat
    12781 tgtttcataa atacttaaaa gcagatgcta tactttttcc tagctacttg gaaagctttg
    12841 gtttaccgtt agcagagggc gcaatgcttg ggaaatatat aatttgtagc gatttgccat
    12901 atgcaagaga tgtgcttaac aattattcaa atgtagaata tataaatcat gatgatgcca
    12961 gtaaatgggc attagctatg aaggaaataa tattaaagaa tagaaataat actttatctg
    13021 aaagtgaaaa tagctatatg tattgtccga aaacaagctg ggcagatttc ttcgagttaa
    13081 tctaattgag gtatacgatg tttaaaaata aggtacttct cattactggt ggcacaggtt
    13141 cctttgggaa tgccgtttta caacgctttt tagattcaga tattggcgaa attcgaatct
    13201 ttagtcgtga tgagaaaaaa caagatgata tgcgtaaaaa atacgcaagt gataagttaa
    13261 aattttatat aggggatgtg agggactata gcagtgtttt aacagcgact cgtggggttg
    13321 attttattta tcatgcagca gcattaaagc aagtaccttc atgtgagttt tatccaatgg
    13381 aagctgtcaa gactaacgtt attggaactg ataacgtact tgaagctgca attgcaaaca
    13441 aagtttcacg tattgtttgt ttgagtaccg ataaagctgt ttatccaatt aatgctatgg
    13501 gtacatctaa ggctatgatg gaaaaagtta tcgtagctaa gtcaagaaac ttacctaaag
    13561 atattactat ttgtgcgacg cgatatggaa atgtaatggc ttcccgtggt tcggtcatcc
    13621 ctctgtttat taatcaaata cttgaaggac gcccaatcac tatcaccgat ccgtgtatga
    13681 ctcgattcat gatgactttg gatgatgctg tagatcttgt attacatgct tttgaacatg
    13741 gtactaatgg tgatattttt gttcaaaagg caccagctgc aacaatagat acacttacca
    13801 agtcattact taagttaact aaacaacatt ctcacccaat taatatcatt ggcactcgcc
    13861 atggtgaaaa actatttgag gtactctgca gtcgggaaga aatgttggtt gcagaagatc
    13921 aaggtaatta ctatcgtata cctagtgata aacgtgatct aaattatgaa aaattcttcg
    13981 acaaagggac taaagaaatt cagtttgttg aagactataa ttctcataat acccgtcgtt
    14041 tagatgttga tgaaatggtt gctcttctta gaaaattaga ttacatcaat aaaattgagg
    14101 ctggtgaaaa ggcagatccc gatgcgtaaa aaaattctaa ttgttggcgc aaatggcatg
    14161 ttaggtagta gtttactacg ctacttttca tcaattggag attatgaagt tcttggtacg
    14221 acaagaagta tggtcgttgc aaaacaactt gagcaaaagc acaatgtgaa aattattgac
    14281 aacgttgatg ttattgattt taaacgatta gagactgtag tagtagagca taagccaaat
    14341 attgttttca actgcgttgg gataattaaa caacttgatg cagcaaaaaa caatatatta
    14401 tctattgaaa ttaactcatt acttccacat aaattagctc aattatgttc agctcatagt
    14461 gctaaactca tacatttttc aacagattgc atttttaaag gtaccaaagg taattatgtt
    14521 gaggatgatg agtctgatgc aattgattta tatggtaaat ctaagttctt gggtgaagtt
    14581 gaatataatg ggcatttaac tttgcggact tctattattg gccatgagtt gggatcaaat
    14641 catagtcttg ttgactggtt tttatcacag aaaaaatcgg tgaaaggatt tactaatgcg
    14701 atattctcag gcctcccaac ttgttatatg gcagaggtta tccataaata tgttcttccc
    14761 aacaatcttg ctggtttatt tcatttaagt gtagagccaa ttagtaaata tgatttgtta
    14821 aatattatta aaatagtata tggagtaagt actgatatag agccaaccaa cgaatttaaa
    14881 atagaccgca gcttgaattc tacgctattt cgtaataaga caaattttgt cccagaatct
    14941 tgggataaat taattgaaaa gatgaaagat gaatacaata aatatttcta attcaaaaat
    15001 acgggttgtt actgtagttg gcactaggcc tgaaatcatt cgtttatcga gggtaattgc
    15061 tgttctcgat gagtacactg aacattttct tgttcatacc ggacaaaact atgattacga
    15121 gcttaatgag gtattcttta atgagcttga aatcagaaaa cctgattttt tcatgaatgc
    15181 cgcgggccag aatgctgctg agaccatcgg aaatgtaatt attgaagctg ataagatatt
    15241 tgataaattg caacctgaag ctttacttat tcttggtgat acaaatagtg ctctagtttc
    15301 cattgcagct aaaagacgaa aaattcctat ctttcatatg gaagcgggca accgatgctt
    15361 tgattacaga gttccggagg aaataaatag gaagattgtt gatcatattt cagatattaa
    15421 tttaacatat agtgagatag ctagggatta tcttttgagg gaagggcttc ctgctgatca
    15481 aataatcaaa accggtagcc ctatgcgaga ggttctaaat ttttataagg ataaaatttc
    15541 atcttcttct attcttgaaa aacttaacct tcaagcatct agttatttcc ttgttagtag
    15601 tcatcgagaa gagaatgttg attcgcctga gaagttgcgc tctcttattg agacgttaaa
    15661 tatagtatct gagaaatata aactgccagt aatagtatca acgcatcctc gtactcgtaa
    15721 tagaattgat gctttaggta tcacggtaag taataatatc attttttcta agccattcgg
    15781 atttttagat tatataaaac tgcagcaaaa tgctcgtgtc gtgctctctg atagtggaac
    15841 tataactgaa gaatcatcag ttttgaattt tccagcctta aacctgcgag aagtacatga
    15901 gagaccagaa ggatttgaag aagctgttgt tatgtttgtc gggctagata gaaacagaat
    15961 tattcaggga atcgatattt tgcagggtca aaaacgtggt gataatgatc gtgaccttca
    16021 tatggttaca gattatcagg cagacaatgt ttcaattaaa attcttagaa ttattatgag
    16081 ctatacaaac tttattaatc aaaaggtttg gaaaaaattc taatgcgtat tgcgttaata
    16141 tgtgatgact atttgcctga tagtactcgt gttagtgcta agatgatgca tgaattggct
    16201 tgtgaacttt tagaaaaagg acatgagcca atcgttattt gtccttgcaa taaaatacag
    16261 acgctcgaaa ttttgaattt ggatggtgtc gtcgtttata agttccctaa tggtgctata
    16321 aagaatgtat ctaaaatatc aagagcaata aacgaatcca tgctatcatt caacgcatgg
    16381 cgatttattg gaaaatatat tcgagaaact aaaattgatg gtgttgtgta ctactcacca
    16441 tctatttttt ttggaaaatt agcaaataaa atcaaagaaa attggcattg taaatcatat
    16501 ttaatcttaa gggattcttt tcctcagtgg ctggtagatc aggggataat taaggaggga
    16561 gggcttgcgg aacgatattt taggtacttc gagcagatca attatgatgc tgcagattat
    16621 attggtttaa tgtcggatag aaacaaagat attttcatta ataaatatca gaataaatac
    16681 aaggtgcaaa ctttattcaa ttgggctgac tttaaaggta tagataatat tcccagtgcg
    16741 actctacgat caaaattagc acttcaaaac aaagtaattt ttttttatgg aggcaatatt
    16801 ggtcatgctc aggatatgat gaatttaatg aggttggtta gatcagcatc ttatcgtgat
    16861 gatgtgcatt ttttattgat tggacaaggt gatgaagttt cccttgtaaa gcagtttatt
    16921 atagataatt ctttaaaaaa ctgtacgtat ttaccatcca taacacaatc agagtttaag
    16981 tctgtattaa agattgttga tgttggtttg tttagccttg ctaaaaatca tactgttcat
    17041 aacttccctg gtaaattgtt agggtatatg gcaaataaac tccctatatt aggtagtgtt
    17101 aatgcaagca atgatgttat ggagattatc aatggggcaa aggccgggtt tgtttttgtt
    17161 aacggaaatg acgaggcttt acttaatgct gcaataaatc ttgcggatga tactcagcta
    17221 agaaaaaacc tgggatgtaa tgcttactct ttattacaag aaaaattctc tgtggaaatg
    17281 gctgctgaaa agatattaag cagcttattt tcctaacagt aatatggtgg ttttatgaaa
    17341 gtgtcttttg taagtcagga tgatttcgag aatttaattc aggaagcatt tgcatccgtt
    17401 cgcttgagat ctcacttact cctccatgaa agccctaatg atgctgtcca gagaataatg
    17461 ataggacttg tcaaaggaac gtatatcccg cctcattttc atgaatttca gcatcaatgg
    17521 gaacatttcc atgtgtttca aggtgaggtt gaattaatgc tatttgatag taacggctgt
    17581 ttaaataaaa aggttattct tggtgggcag agtaagaata ttattgcaca aatttcacca
    17641 ctcaccccac atacactagt ttgtagatct cctacagcag ttatcatgga gattaaagaa
    17701 ggtccttttg atgaaaaatg cgctaaagtc atcccctcat ggtcatatag tgaagattat
    17761 tccattttat ccagagatag gatcatcgca atgatgggcc aattatcaat tggagataga
    17821 tttagtctct aaacttcaca gcaattagcg aagtaatctt ctttttcata ccttctatat
    17881 tatatttatc cactactatt atagctttga atactgagtt aacatctaca ctacattcaa
    17941 gccgtgcata cgtcgcggtg accacccctg acaggagtaa ataatgtcaa agcaacagat
    18001 tggcgtagtc ggtatggcag tgatggggcg caaccttgcg ctcaacatcg aaagccgtgg
    18061 ttataccgtc tctattttca accgttcccg tgaaaagacg gaagaagtga ttgccgaaaa
    18121 tccgggcaag aaactggttc cttactatac ggtgaaagag tttgttgaat ctctggaaac
    18181 gcctcgtcgc atcctgttaa tggtgaaagc aggtgcaggc acggatgctg ctattgattc
    18241 cctcaaacca tatctcgata aaggcgacat catcatcgat ggtggtaaca ccttcttcca
    18301 ggacaccatt cgtcgtaacc gtgagctttc cgcagaaggc tttaacttca tcggtaccgg
    18361 tgtttccggt ggtgaggagg gcgcactaaa aggtccttcc attatgcctg gtgggcagaa
    18421 agaagcctat gaacttgttg cgccgatcct gaccaaaatc gccgcagtgg ctgaagacgg
    18481 tgagccatgc gttacctata ttggtgccga tggcgcaggt cactatgtga agatggttca
    18541 caacggtatt gaatacggcg atatgcagct gattgctgaa gcctattctc tgcttaaagg
    18601 tggtctgaac ctcaccaacg aagaactggc gcagaccttt accgagtgga ataacggtga
    18661 actgagcagc tacctgatcg acatcaccaa agatatcttc accaaaaaag atgaagacgg
    18721 taactatctg gttgatgtga tcctggatga agcagcaaac aaaggtacgg gcaaatggac
    18781 cagccagagt gcgctggatc ttggcgaacc gctgtcgctg attaccgaat ctgtgtttgc
    18841 acgttatatc tcttctctga aagatcagcg tgttgccgcg tctaaagttc tctctggtcc
    18901 gcaagcgcag ccagcaggcg acaaagctga gttcatcgaa aaagttcgtc gtgctctgta
    18961 tctgggcaaa atcgtttctt acgctcaggg cttctctcag ctgcgagcgg cgtctgaaga
    19021 gtacaactgg gatctgaact acggcgaaat cgcgaaaatt ttccgtgctg gctgcatcat
    19081 ccgtgcgcag ttcctgcaga aaatcaccga tgcttatgcc gaaaatccgc agatcgctaa
    19141 cctgctgctg gctccgtact tcaagcaaat tgccgatgac taccagcagg cgctgcgtga
    19201 tgtcgttgct tatgcagtac agaacggtat cccggttccg accttcgcgg ctgcggttgc
    19261 ctattatgac agctaccgcg ccgcagttct gcctgcaaac ctgatccagg cacagcgtga
    19321 ctatttcggt gcacatactt ataagcgcat tgataaagaa ggtgtgttcc atactgaatg
    19381 gctggattaa tctgatttaa ccaacaataa aattgaggcc cggcgtatat tgcaccgggc
    19441 ttttttttgc catttatctt atagactaaa tttcactgct taatattaac ttaataaata
    19501 tcagctattc ttataaagaa aatctgaatt gtttttcgtt gcgttgacca tctgacaacg
    19561 tagcgttaaa acttttagct cttatcagga tgctaaaaac atcatgattc acagttaagt
    19621 taattctgag agcatgaaat gaaaatcacc atttccggta ccggctatgt aggcttgtca
    19681 aacgggcttc taatcgcaca aaatcatgag gttgtggcat tagatatttt accgtcacgc
    19741 gttgctatgc tgaatgatcg gatatctcct attgttgata aggaaattca gcattttttg
    19801 caatcagata aaatacactt taatgccaca ttagataaaa atgaagccta ccgggatgct
    19861 gattatgtca tcatcgccac tccaaccgac tatgatccta aaactaatta tttcaataca
    19921 tccagtgtag aatcagtaat taaagacgta gttgagataa atccttatgc ggttatggtc
    19981 atcaaatcaa cggttcccgt tggttttacc gaagcgatgc ataagaaata tcgtactgaa
    20041 aatattattt tctctccgga attcctccga gaaggtaaag ccctttacga taatctccat
    20101 ccttcacgta ttgtcatcgg tgagcgttca gaacgcgcag aacgttttgc ggcgttatta
    20161 caggaaggcg cgattaagca aaatatcccg accctgttta ccgactccac tgaagcagaa
    20221 gcgattaaac ttttcgctaa tacctatctg gcgatgcgcg tagcatactt taatgaactg
    20281 gatagctatg cagaaagctt agggctgaat actcgccaga ttatcgaagg cgtttgtctc
    20341 gatccgcgta ttggcaacca ttacaacaac ccgtcgtttg gttatggtgg ttattgtctg
    20401 ccgaaagata ccaagcagtt actggcgaac taccagtctg tgccgaataa cctgatctcg
    20461 gcaattgtcg atgctaaccg cacgcgtaaa gactttattg ccgatgccat tttgtcacgc
    20521 aagccgcaag tggtgggtat ttatcgtctg attatgaaga gcgggtcaga taacttccgt
    20581 gcgtcttcca ttcaggggat tatgaaacgt atcaaggcga aaggcgttga agtgatcatc
    20641 tacgaaccgg tgatgaaaga agattcattc ttcaactctc gcctggaacg tgatctcgcc
    20701 accttcaaac aacaagccga cgtcattatc tccaaccgta tggcagaaga acttaaggat
    20761 gtggcagaca aagtctacac ccgcgatctc tttggaagcg actaacatcc tgttatcagg
    20821 gcgattttcg ccctgattct cttatgttcc ctttgtaata attcattatt tttatcattt
    20881 atcctatagc attcatggcg attatcgcta aactatggcg acttaaaatt atttcgtccg
    20941 ttagggtaat gatgagagta gaaaataata atgtttctgg gcaaaaccat gacccggaac
    21001 agattgattt gattgattta ctggtgcagt tgtggcgtgg caagatgaca attatcatct
    21061 ctgtcattgt tgctattgcc ctggctatag ggtatctggc cgttgccaaa gaaaagtgga
    21121 cttccacagc tatcgttact caacctgatg tggggcaaat cgctggttat accaacgcga
    21181 tgaatgtgat ctacggatcg gctgtaccga aagtctccga cattcagact tcacttatcg
    21241 gacgttacag cactgcattc tcagcattag cggaaacgct ggataaccag gaagaagcag
    21301 agaagctgac cattgagcct accgttaaaa accagccgtt accactggcg gtgtcttatg
    21361 ttggtgaaac acctgaaggt gcgcagaaac agttggcgaa gtatatccag caagttgacg
    21421 accaggttaa tgaagaactg gaaaaagacc tgaaagacaa catcgcgctg cgtatgaaaa
    21481 acttgcagga ttctctgaaa actcaggaag tagtcgcgca ggagcagaaa gatctgcgta
    21541 tccgtcagat tcaggaagcg ttgcagtatg cgaatcaggc gcaggtgaca aagccacagg
    21601 ttcagcagac tgaagatgtg acacaagata cgttgttcct tttggggagc gaagcgctgg
    21661 agtcgatgat taagcacgag gcgacccgtc cgttggtatt ctcaccaaac tactatcaga
    21721 cacgtcaaaa cctgctggat atcgaaagct taaaagttga tgatcttgat attcatgctt
    21781 accgctatgt aatgaaaccg acgttacctg ttcgtcgcga tagcccgaaa aaggcaatta
    21841 ccttgattct ggcggtgctg ctgggcggca tggttggcgc ggggattgtg ctggggcgta
    21901 acgctctgcg taattacaac gcgaagtaat cttttcggtt ttaaagaaaa tgggcagggt
    21961 ggtgacacct tgcccatttt tttggccgga tgcgacaaca atatcgcatc cgctcagccc
    22021 gcaacttact gatgccgttt acgcaggttc tcaatcaccg ttgttaaatc cagatcctga
    22081 tcctgcaaca acaccagcag gtgatacatc aaatcagatg cctcgttggt cagctcaaag
    22141 cggtcatgta ccgttgctgc cagtgcggtt tccacgcctt cttcgcccac tttctgcgca
    22201 atgcgcttgg tgccgctggc atacagtttg gcagtgtagg aggtttccgg gtcggcagat
    22261 ttacgttcgg cgagcagttg ttccagttga tagaggaaca gccactggtg agcggtgtcg
    22321 ccgaagcagc tgctggtgcc tttgtggcag gttggcccga tgggattcgc cagcaccagt
    22381 aacgtgtcgt tgtcgcagtc cggcgcaata ctcaccacat tgaggaaatt gcccgacgtt
    22441 tcgcctttgg tccacagtcg ctgtttagtg cgcgagaaga aggtgacttt gccgctttcg
    22501 atggttttgt ccagagcttt cgggttcata tagcccagca ttaacacttc gccagatacc
    22561 gcgtgttgca caatcaccgg cataagtccg tcggtttttt cccagtccag ttcgcgacgt
    22621 tgttgttctg ttaacatatc ctgatctcca cgccctgtgt tgccaggtac gcttttaatt
    22681 caccaatatt gattatttgt ttgtggaata cggaagctgc cagcgcgccg tcaacgtcgg
    22741 catcgcggaa ggcttcgagg aagtgttcca tggtgcccgc gccaccggaa gcaatcagcg
    22801 ggacgtggca aacttcacgc acttttttca gttgttcgag gtcgtaaccg ttacgcacgc
    22861 cgtcctgatt catcatgttg aggacgattt ctccggcacc gcgtttttgc acttcctgta
    22921 cccagtcgag c
//
