LOCUS       GU299792               15394 bp    DNA     linear   BCT 08-JUN-2010
DEFINITION  Escherichia coli strain G1674 O antigen gene cluster, complete
            sequence.
ACCESSION   GU299792
VERSION     GU299792.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15394)
  AUTHORS   Li,D., Liu,B., Chen,M., Guo,D., Guo,X., Liu,F., Feng,L. and Wang,L.
  TITLE     A multiplex PCR method to detect 14 Escherichia coli serogroups
            associated with urinary tract infections
  JOURNAL   J. Microbiol. Methods 82 (1), 71-77 (2010)
   PUBMED   20434495
REFERENCE   2  (bases 1 to 15394)
  AUTHORS   Li,D., Liu,B., Chen,M., Guo,D., Guo,X., Liu,F., Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..15394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G1674"
                     /db_xref="taxon:562"
                     /note="serogroup: O2"
     misc_feature    1..15394
                     /note="O antigen gene cluster"
     gene            105..1190
                     /gene="rmlB"
     CDS             105..1190
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ADC54932.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     EVSDSERYSFEHADICDAEAMARIFSQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSGLDDEKKKNFRFYHISTDEVYGDLPHPDEVNNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESWIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            1190..2089
                     /gene="rmlD"
     CDS             1190..2089
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ADC54933.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTNYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPDFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGSGDAPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2148..3023
                     /gene="rmlA"
     CDS             2148..3023
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ADC54934.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEDFIGG
                     DDCALVLGDNIFYGHDLPKLMEAAVNKESGATVFAYHVNDPERYGVVEFDNNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     IKNQYGQYLLKMISE"
     gene            3038..3451
                     /gene="fdtA"
     CDS             3038..3451
                     /gene="fdtA"
                     /note="dTDP-6-deoxy-3,4-keto-hexulose isomerase"
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA"
                     /protein_id="ADC54935.1"
                     /translation="MMDIKLISLQKHGDERGALIALEEQRNIPFEVKRIYYILETLNG
                     VRRGFHAHKVTRQLAIVVKGACKFHLDNGKETKQVELNDPTIALLIEPYIWHEMYDFS
                     DDCVLLVIADDFYKESDYIRNYDDFIRRVNSIENS"
     gene            3510..3905
                     /gene="fdtC"
     CDS             3510..3905
                     /gene="fdtC"
                     /note="dTDP-D-Fucp3N acetylase"
                     /codon_start=1
                     /transl_table=11
                     /product="FdtC"
                     /protein_id="ADC54936.1"
                     /translation="MILKGAVIGNNCNICANTLIENNVVIGNNVTVKSGVYIWDGVKI
                     EDNVFIGPCVAFTNDKYPRSKVYPDEFLQTIIRKGASIGANATILPGIEIGEKAIVGA
                     GSVVTKNVPPCAIVVGNPARFIKWVEDNE"
     gene            3898..5004
                     /gene="fdtB"
     CDS             3898..5004
                     /gene="fdtB"
                     /note="dTDP-6-deoxy-D-xylo-hex-3-ulose aminase"
                     /codon_start=1
                     /transl_table=11
                     /product="FdtB"
                     /protein_id="ADC54937.1"
                     /translation="MNKIDFLDLFAINQRQHKELVSAFSRVLDSGWYIMGEELEQFEK
                     EFAEYCGVKYCIGVANGLDALILVLRAWKELGYLEDGDEVLVPANTYIASILAITENK
                     LVPVLVEPDIETYNINPALIENYITEKTKAILPVHLYGLLCNMPEISAIARKYNLLIL
                     EDCAQAHGAIRDGRKAGAWGDAAGFSFYPGKNLGALGDAGAVTTNNAELSSTIKALRN
                     YGSHKKYENIYQGLNSRLDELQAALLRVKIHTLPEDTAIRQRIAEKYIREIKNPAITL
                     PVYEGQGAHVWHLFVVRIANREKFQSYLLEKGIKTLIHYPLPPHKQQAYQNMSSLSLP
                     ITEQIHDEVISLPISPVMSEDDVNYVIKMVNDYK"
     gene            5004..6266
                     /gene="wzx"
     CDS             5004..6266
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADC54938.1"
                     /translation="MKKFLQVTILSAIYTFIKMIAGFIIGKVVAIYTGPSGVAMLGQV
                     QSLITIVAGTTSAPVSTGLVRYTAENWQEGQEACAPWWRACLRVTLFLFLLIIPVVII
                     LSKNISELLFSDGQYTWLIIFACCILPFSIINTLIASVLNGQQFYKQYILVGMFSVFI
                     STMFMILLIVAYNLKGALIATAINSAIAGLVLVLFCLNKSWFRFKYWWGKTDKDKIIK
                     IIHYTLMALVSVISMPTALMCIRKILIAKTGWEDAGQWQAVWKISEVYLGVVTIALST
                     YFLPRLTIIKTSFLIKKEVNSTILYIISITSFMALSIYLFRDLVITVLFTEQFRSARE
                     LFLLQLIGDVIKIAGFLYAYPLQSQGHTKLFISSEVIFSMLFIITTYIFVVNYGVHGA
                     NISYVITYSLYFVFAFVFTNFINVRRNN"
     gene            6361..7332
                     /gene="wekP"
     CDS             6361..7332
                     /gene="wekP"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WekP"
                     /protein_id="ADC54939.1"
                     /translation="MNYGHDVSFVVPDNRTNPYYATTAKIVTSKSSQNRVKILRIIKN
                     YYNLWRKCIELNPDAVVASFHLTAYLVALLPITRRKKYYYIQAYEVNFFDNIIWKLIA
                     GLTYYLPLKKILNSPNLLPHKHDDFIGVVPAGVDLNVFYPKPSNRLLNGHTSIGIIGR
                     KEKHKGTSEIISVLCSLENKAGIIINIAIYLEEVDKQRLIAAGFQVNFFPITSDLELA
                     SFYRSNDIMIAVGLIEDGAFHYPCAESMACGCLVISNYAPLTETNSVLKLVKFDACKL
                     GEAINLCLNLDLEEKSKEIQSNISVLNKYDWKIVGETFNSLLLDANK"
     gene            7353..7910
                     /gene="rmlC"
     CDS             7353..7910
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ADC54940.1"
                     /translation="MNIVKTDIPDLIVLEPKVFSDERGFFMESYNQIEFEKAIGRHVN
                     FVQDNHSKSSKGVLRGLHYQLAPYAQAKLVRCVVGQVFDVAVDLRKNSPTFKKWFGIT
                     LSAENKRQLWIPEGFAHGFLVTSDEAEFIYKTTNYYAPGHQQAIIYNDPILNIDWPFC
                     SSALSLSQKDQEAKLFSELLDSELF"
     gene            7980..8942
                     /gene="wzy"
     CDS             7980..8942
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADC54941.1"
                     /translation="MTENSPKYKHDKSLISFIYLFFIFTLIVGFIIANTQFLGRSRDY
                     DNYIQIFSGKEGEGVLELFYRGLMLITTSYETIIFIILTCSFFIKARFLANYSRNFSG
                     LTLFFIYYASVALWVLDYTQFRNGLCISILMFSVYYLFINKPTYFYFSVLCAIATHWS
                     ALPFLLLYPFVYSTKIRRLGYFCFSILVLIAISGEGKEIISFIRNFGVGQKIGNEAGV
                     NLINSLSLTAISWFIISYISSIGNERRNLRLFFCYGVMQYVTFSLFSLPVMAFRILEM
                     YFFLMLTIGVFIKQKKNYYFVFCKVLILLYLTYYYHMVFGVINV"
     gene            8935..9822
                     /gene="wekQ"
     CDS             8935..9822
                     /gene="wekQ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WekQ"
                     /protein_id="ADC54942.1"
                     /translation="MCKAKVLAIIVTYNPEIIRLTECINSLAPQVERIILVDNGSNNS
                     DLIKNISINNLEIILLSENKGIAFAQNHGVKKGLEAKEFDYLFFSDQDTCFPSDVIEK
                     LKSTFTKNNKKGKNVACASPFFKDHRSNYMHPSVSLNIFTSTKVICSEVDDDLYPPHV
                     IASGMLMSREAWRVVGPFCEKLFIDWVDTEWCWRALANNMIIVQTPSVIISHELGYGQ
                     KIFAGRSVTIHNSFRNFYKIRNAIYLMLHSNYSFKYRYHAFFHATKNVVFEILYSKEK
                     LNSLKVCFKAVRDGMFNNF"
     gene            9853..10929
                     /gene="wbuV"
     CDS             9853..10929
                     /gene="wegR"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WegR"
                     /protein_id="ADC54943.1"
                     /translation="MEENNMKTVAVVGTVGVPACYGGFESLVQNLIDYQSDGIQYQIF
                     CSSKKYDKKFKNYKNAELIYLPINANGVSSIIYDIMCLIICLFKRPDVVLILGVSGCL
                     FLPIYKLFSKSKIIVNIDGLEWRRNKWGTFAKKFLKISEAISIRIADIIISDNQAIAD
                     YVENKYKKKSVVIAYGGDHATNLSTPIDNDQKKEGYYLGLCRIEPENNIEMILNAFIN
                     TDKKIKFMGNWDNSEYGRQLKKYYSNYPNITLLEPNYNIEELYKLRKNCLAYIHGHSA
                     GGTNPSLVEAMHFNIPIFAFDCDFNRYTTNNLAHYFNDSEQLSLLAESLSFGNLKCRV
                     LDLKNYAEDMYNWRHIAAMYESIY"
     gene            13682..15235
                     /gene="wekS"
     CDS             13682..15235
                     /gene="wekS"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WekS"
                     /protein_id="ADC54944.1"
                     /translation="MDSVFLFSIEYTFISVAIFLIKRKFVSNILWVIYLSILGVQAAS
                     LFSSGQYLMPLALSNAAEIESLGIGEVIKVFFVFILFIIISFITLPSGALTERIKYYK
                     FNLLCIVLLLVCSFISAGPVYAFYETAKLFYLQQTFKPSKKIGDAGKFFLKTSVFINP
                     IENNYSFKGRNVIVIFAEGFSSEIMGNAKPRAFSVTPNLDKLSTEALSFKNYYNHTAA
                     TFRGLRGQLTSGYQFRDGLTDAGTGIAQLSKAEINNIYLHRQTSLPDILKEYGYKSYF
                     IASTEKNSPLNTMLKTLNFDKVLGMGDFIGYQRDRMTDKQTFNALQYFLRSQENKKGP
                     FFIGVYPSGTHHGQDSPNEKYFDGNNPLYNKFYNYDFQLGKFIDFFRRSSFYNNTLLI
                     ITSDHSTFPSTEYKKAFNSDSRYFVGEIPFLVIGGDITPEILDADGKNSLSFAPTILH
                     MLGIQYSMNYFLGCSLIDKECTSPFSHLSAIGSDYFVTSKENHVELIPNNNDSELIKL
                     AESFYNISG"
BASE COUNT     4870 a   2461 c   3145 g   4918 t
ORIGIN
        1 atgtagctgt aagccaaggg cggtagcgtg cattaatact tctattaatc aaatctcgag
       61 cagtctattt cacagtatgc tctctggcta tatggaataa aaaagtgaag atacttgtta
      121 ctggtggcgc aggctttatt ggttctgctg ttgttcgtca cataataaat aatacgcaag
      181 atagtgttgt taatgtcgat aaattaacat acgccggaaa cctggaatcg ctcgctgaag
      241 tttctgattc tgagcgttat tcatttgagc atgcagatat ctgcgatgcc gaagcgatgg
      301 cgcgtatttt ttcacaacac cagccagatg cggtgatgca ccttgcggct gagagccacg
      361 ttgaccgctc aataactggc ccggcggcat tcattgaaac taatattgta ggtacttacg
      421 tacttttaga agctgcgcgc aattattggt ctggtctgga tgatgaaaag aaaaaaaact
      481 tccgctttta tcatatttct actgatgaag tatatggtga cttaccccat ccggatgaag
      541 taaataataa cgaagcgtta ccgctattta cggaaacgac agcctacgcg ccaagtagcc
      601 cgtattctgc ttctaaagct tccagcgatc atttggttcg cgcatggaaa cgtacgtatg
      661 gtttaccgac cattgtgact aattgctcga acaactatgg tccgtatcac ttcccggaaa
      721 agcttattcc attggttatt cttaatgcac tggaaggtaa ggcattacct atttatggca
      781 aaggggatca aattcgcgac tggttgtatg tagaggatca tgctcgtgcg ttatataccg
      841 tcgtaaccga aggtaaagcg ggtgaaactt ataacattgg cggacacaac gaaaagaaaa
      901 acatcgatgt tgtgctgact atttgtgatt tgttggatga gattgtaccg aaagagaaat
      961 cttatcgtga gcaaattact tatgttgctg atcgcccagg gcatgatcgc cgttatgcaa
     1021 ttgatgccga taaaattagc cgcgaattgg gctggaaacc acaggaaacg tttgagagct
     1081 ggattcgcaa aacggtggaa tggtatctgg ctaatacaaa ttgggttgag aatgtgaaaa
     1141 gcggtgctta tcagtcatgg atcgaacaaa actatgaggg ccgtcagtaa tgaatatcct
     1201 gcttttcggc aaaacagggc aggtgggttg ggaactgcag cgtgctctgg cgccgctggg
     1261 taatctgatc gctcttgatg ttcactccac taattattgt ggagatttca gcaaccccga
     1321 aggtgtggca gaaaccgtca aaaaaattcg tcctgacgtt attgttaatg ctgctgctca
     1381 cactgcagta gataaagcag aatcagaacc ggatttcgca caattactta acgcgacaag
     1441 cgtcgaagcg attgcaaaag ctgctaatga agtcggggcc tgggttatac actactctac
     1501 tgattatgtt ttcccaggca gtggtgacgc gccatggctg gaaacggatg caacagcacc
     1561 gctaaatgtt tacggtgaaa caaaattagc tggggaaaag gcattacaag aacattgcgc
     1621 aaagcatctt attttccgta ccagctgggt atacgctggt aaaggaaata actttgctaa
     1681 aacgatgttg cgtttggcaa aagaacgcga agaactggct gtgataaacg atcagtttgg
     1741 cgcaccaaca ggtgctgaat tgctggctga ttgcaccgct catgccattc gcgtggcatt
     1801 aaaaaaacca gaagtcgctg gcttgtacca tctggtagca agtggcacaa caacctggca
     1861 cgattatgct gcgctggttt ttgaagaggc gcgcaaagca gggattaatc ttgcacttaa
     1921 caaacttaac gccgtgccaa caacggccta tcccacacca gcccgtcgac cccataactc
     1981 tcgcctcaat acagaaaagt ttcagcagaa ctttgcgctt gtcttgcctg actggcaggt
     2041 gggcgtgaaa cgtatgctca acgaattatt tacgactacg gcaatttaac aaatttttgc
     2101 atctcgctca tgatgccaga gcgggatgaa ttaaaaggaa tggtgaaatg aaaacgcgta
     2161 aaggtattat tctggctggt ggttccggca ctcgtcttta tcctgtgacg atggcagtga
     2221 gtaaacaatt gctgccgatt tatgataagc cgatgattta ttatccgctt tcaacgctta
     2281 tgttagcggg tattcgcgat attcttatta ttagtacgcc acaggataca ccgcgtttcc
     2341 aacaattatt gggggacggg agccagtggg gtcttaatct acagtataaa gtacaaccga
     2401 gtccggatgg cctggcgcaa gcgtttatta ttggcgaaga ctttattggt ggtgatgatt
     2461 gtgcactcgt acttggcgat aatatcttct atggacacga cttgccgaaa ttgatggaag
     2521 ctgctgttaa caaagaaagc ggtgcaacgg tatttgctta tcacgttaat gatcctgaac
     2581 gctatggtgt cgtggagttt gataataacg gtacggcaat tagcctggaa gaaaaaccgc
     2641 tggagccaaa aagcaactat gcggttactg ggctttattt ctatgacaat gacgttgtgg
     2701 aaatggctaa aaaccttaag ccttctgccc gtggcgaact ggaaattacc gatattaacc
     2761 gtatttatat ggaacaagga cgtttgtctg tagccatgat ggggcgtggc tatgcatggt
     2821 tggatacagg gacgcatcaa agccttattg aagcaagtaa cttcattgca acaattgaag
     2881 agcgtcaggg attaaaggta tcttgcccgg aagagattgc ttaccgtaaa gggtttattg
     2941 atgccgagca ggtgaaagta ttagccgaac cgcttatcaa gaatcaatat ggtcaatatt
     3001 tgctgaaaat gatcagcgaa tagtatatgg gaactcaatg atggatatta aattaatctc
     3061 tttgcaaaaa catggggatg agcgcggtgc attaattgct cttgaagagc aacgaaatat
     3121 acctttcgaa gtcaaaagaa tatattacat acttgagact cttaatggag taagacgcgg
     3181 atttcatgcg cacaaggtta ctcgtcagtt agctattgta gtcaagggag cttgtaaatt
     3241 tcatctggat aatggtaaag aaacaaagca ggtggaactt aatgatccaa caattgcgtt
     3301 gctgatagaa ccctatatat ggcatgaaat gtatgatttt agtgatgatt gtgtgctgct
     3361 tgtaattgcg gatgatttct ataaagagtc tgattatatc cgcaattatg atgattttat
     3421 tagaagagta aattcaattg agaattcata agctaagtga cgtccagaca acatcaattg
     3481 gtgatggaac aactatctgg cagtttgttg tgatactaaa aggtgctgta attggtaata
     3541 attgcaacat ctgtgcaaat accttaattg aaaataacgt tgtaattggt aacaatgtca
     3601 cagtcaaaag cggtgtgtat atttgggatg gcgttaaaat agaggataat gtttttattg
     3661 gtccttgtgt agcatttaca aatgataagt atcctcgctc taaagtctat cctgatgaat
     3721 ttttgcaaac aataatacgc aaaggagcat caataggtgc taacgcaacc atcctgccag
     3781 gaattgaaat tggtgaaaaa gcaatcgttg gtgcggggag tgttgtaacc aaaaatgtac
     3841 cgccatgcgc aatagtagta ggtaatccag ctcgatttat taaatgggta gaggataatg
     3901 aataaaattg attttttaga tctttttgca attaaccagc gacagcacaa agaattagtc
     3961 tctgcgttta gtagggtgct agattctggt tggtatatca tgggcgaaga acttgagcag
     4021 ttcgagaaag agttcgcaga atactgtgga gttaagtatt gcattggtgt agcaaatggc
     4081 cttgatgcgt tgatactagt attgagggca tggaaagaac ttggctatct tgaagacggt
     4141 gacgaggtat tagtaccggc aaatacatat attgcttcta ttcttgctat aacagagaac
     4201 aaacttgttc ctgttcttgt tgaaccagat atagaaactt ataatattaa tcctgcttta
     4261 attgaaaatt acattacgga aaaaactaaa gcaatattac cggttcactt atatggtcta
     4321 ttgtgcaata tgccagaaat tagtgcaatc gccagaaaat ataatctgtt gattcttgaa
     4381 gattgtgcac aagcacatgg tgcaatacgt gatggtcgca aagctggagc ttggggggat
     4441 gctgcaggat ttagttttta tccaggaaaa aaccttggag ctttggggga tgcgggagct
     4501 gttactacaa ataatgcaga attatcctca actataaaag ctttgcgaaa ttatgggtca
     4561 cataagaaat atgaaaatat ttatcaggga ttgaatagtc gattggatga actgcaagca
     4621 gccttattgc gtgtaaaaat ccatacatta ccggaagata ctgcgattcg gcaaaggatt
     4681 gctgaaaaat atattcgtga aataaaaaac cctgcgatta cgttaccagt gtacgaaggc
     4741 caaggtgcgc acgtttggca tttatttgta gtaagaatcg ctaatcgtga aaaattccag
     4801 tcatacttat tagagaaggg tatcaaaacc ttaattcact atccattacc accccataag
     4861 cagcaagcat atcaaaatat gtctagcctt agccttccaa ttactgagca aattcatgat
     4921 gaagtcattt ctttacctat aagtccggta atgagtgaag atgatgtcaa ttatgtaatc
     4981 aaaatggtca atgattacaa gtaatgaaaa aatttcttca ggtaactata ttatccgcta
     5041 tctatacatt cattaaaatg attgcgggtt ttatcatcgg taaggtagta gcaatttata
     5101 cagggccatc aggggtagca atgcttggcc aagtgcaaag tttaatcaca atagttgcag
     5161 gtactacctc tgcacctgta agcactggcc ttgttcgata tactgcggaa aattggcaag
     5221 aaggacaaga agcatgcgcg ccatggtggc gcgcatgctt aagggttact ctgtttttat
     5281 tcttgcttat tattcccgtt gttattatat tgtcgaaaaa tattagtgag ttacttttta
     5341 gcgatggaca atacacatgg ttaatcattt tcgcatgttg tatattgcca ttctccatta
     5401 taaatacatt gatcgcttca gttttaaatg gtcaacaatt ttataagcaa tatatattgg
     5461 ttgggatgtt ttctgtattc atttctacta tgtttatgat tttgttgatt gtagcttata
     5521 atcttaaagg tgcattgatt gccacagcta taaatagtgc tattgctggt cttgtattgg
     5581 ttttattttg tctcaataaa tcttggttta gatttaaata ttggtggggt aaaacggata
     5641 aagacaaaat tataaaaatt attcattata ctctgatggc tctggtttct gttatctcca
     5701 tgcctacagc attgatgtgt attagaaaaa tattgattgc taaaactggt tgggaggatg
     5761 cagggcaatg gcaggccgta tggaagatat ctgaggttta tcttggtgtt gtgacaattg
     5821 ctttgtcaac atatttctta ccaagattga caattataaa aacaagtttc cttataaaaa
     5881 aagaagtaaa tagtactata ttatacataa tatctattac ttcattcatg gcgttgagta
     5941 tctatttatt ccgcgatttg gtaataacag ttttatttac tgaacagttt cgctcagctc
     6001 gtgaattatt tttattacaa cttatagggg atgtaataaa aattgctggg tttctttatg
     6061 cataccctct tcaaagtcag gggcatacta aactattcat cagttcagaa gtgatttttt
     6121 ctatgctctt tatcattacc acctatattt ttgttgtaaa ttatggagta catggtgcta
     6181 acataagtta tgtcattaca tatagtttat attttgtgtt tgcatttgtg tttactaatt
     6241 ttattaatgt tagaagaaat aattaaaaac agaggttgaa ttttgaaaat aattatacct
     6301 gtcttaggat ttggcagggc tggtggtgaa agagttcttt ctaagctggc aactgaattg
     6361 gtgaattatg gacatgatgt aagttttgtt gttccagata atagaactaa tccatattat
     6421 gctaccacag caaaaattgt cacgagtaaa tctagtcaaa accgtgtaaa aatattgaga
     6481 atcattaaaa attactataa tctgtggcgt aaatgcatag agttaaatcc tgatgctgta
     6541 gttgctagtt ttcatttgac tgcctatctt gtcgcattat taccaatcac ccgtcgtaag
     6601 aaatattatt atattcaggc gtatgaagtt aatttttttg ataatataat atggaaatta
     6661 atagcgggtt taacatatta tttaccgctt aaaaaaatac taaatagtcc taatttgctt
     6721 cctcataaac atgatgattt tataggagta gttcctgcag gagtagattt aaacgttttc
     6781 tatccgaaac catcaaatag gttattaaat ggtcacacat caatagggat tattggtaga
     6841 aaagagaagc acaaaggaac tagcgaaatt atttcagtat tgtgttcact ggaaaataaa
     6901 gctggaatta taatcaatat tgcgatctat cttgaagaag ttgataagca gcgtttaatc
     6961 gctgccgggt ttcaggttaa tttttttccg attacttctg atttagaatt ggcatccttt
     7021 tatcgaagca atgacatcat gattgctgtt gggttaattg aagatggcgc tttccattat
     7081 ccttgtgctg aatcaatggc ttgtggttgt cttgttattt caaattatgc gccacttact
     7141 gaaactaaca gtgtacttaa attagtcaag tttgatgctt gcaaacttgg tgaagcaatt
     7201 aatctttgtc tcaatcttga cctagaagaa aaaagcaaag aaatccaatc taatatttct
     7261 gtgttgaata aatatgactg gaaaattgtt ggtgaaactt tcaatagttt attgttagat
     7321 gcaaataaat agtatacgtt gatggggaaa atatgaatat tgttaaaact gatattccag
     7381 atctgatcgt tcttgaacca aaagtgttta gtgatgaacg cggctttttt atggagagtt
     7441 ataatcagat tgaatttgag aaggcaatag gaaggcacgt aaattttgtt caggataatc
     7501 attcaaaatc tagtaaaggc gtactacgtg ggttgcatta tcaattagca ccgtatgcac
     7561 aggctaaatt agttcgatgt gttgtaggtc aggtatttga tgttgctgtt gatcttagaa
     7621 aaaattcacc aacgttcaaa aaatggtttg gaataaccct ttccgcagaa aataaacgac
     7681 aattatggat acccgaagga tttgctcatg gtttcttggt gaccagtgat gaagctgagt
     7741 tcatttataa gacaactaac tactatgctc ctggtcatca gcaagcaatt atttacaatg
     7801 atcctatttt aaacatcgat tggcctttct gcagtagtgc tctgtcatta tcacaaaaag
     7861 atcaagaagc aaaattattt tcagaattat tggacagtga actgttctaa taaagtgtgc
     7921 caccttatcc gtctgaagga taggtggttg cttatatttt ttttgagtat gtttgtataa
     7981 tgacagaaaa tagtccgaaa tataaacacg ataaaagctt aataagtttt atctacttat
     8041 tttttatatt tacacttatt gtaggcttta ttatcgcaaa tacccagttt ttggggcgaa
     8101 gtagagacta tgataattat atacagatct tttctggtaa agaaggggag ggggttcttg
     8161 aattatttta tcgcggattg atgttaataa cgaccagcta tgaaactatc atttttataa
     8221 ttttaacatg ttcttttttt ataaaggcaa ggtttctcgc taactattcg cgtaattttt
     8281 caggcttgac cttattcttt atttattatg caagcgttgc actttgggtt ttagattata
     8341 ctcaattcag aaatggtcta tgtatttcca ttttaatgtt ttccgtatac tatttattta
     8401 taaataaacc gacttatttt tatttctcgg tattatgtgc aattgcaact cattggtctg
     8461 ctttgccttt tttgctttta tatccttttg tctattcaac aaaaataaga cgccttggtt
     8521 atttttgttt cagtattctt gttttgattg cgatctcagg agaaggaaaa gagatcatat
     8581 cttttataag aaattttgga gtgggacaaa aaataggaaa tgaagctggt gtaaatttaa
     8641 taaattcatt atcccttacc gctatttcct ggtttattat tagttacata tcaagcattg
     8701 gaaatgaaag gagaaattta aggcttttct tttgttatgg tgtcatgcaa tacgtgactt
     8761 ttagcctttt ctctctacct gttatggctt tccgtatttt ggaaatgtat tttttcctta
     8821 tgctaaccat tggggtgttt attaagcaaa aaaagaatta ttattttgtt ttttgcaaag
     8881 tgttaatttt attgtatcta acatactatt atcatatggt ctttggagtg attaatgtgt
     8941 aaggctaagg tgttggctat aattgttact tacaacccgg aaattattcg attgacggaa
     9001 tgtattaact ctttagcccc acaagttgag agaataattc ttgtagataa tggctcaaat
     9061 aatagtgatt tgataaaaaa tatcagtatt aataaccttg aaattatttt actttcggaa
     9121 aacaaaggca ttgcatttgc tcagaaccat ggtgttaaga agggcctgga agcaaaagag
     9181 tttgactatt tatttttctc agatcaggat acttgctttc ctagcgatgt tattgaaaaa
     9241 cttaagagta catttacgaa aaataataaa aaaggtaaaa atgttgcttg tgcttctcct
     9301 ttttttaaag accatcgttc aaattatatg catccgtcag tcagcctaaa tatttttacg
     9361 agtacaaaag ttatatgtag tgaagtagac gatgatcttt atcccccgca tgttattgct
     9421 tctgggatgt taatgtctcg tgaagcatgg cgcgtcgtcg gaccattttg tgaaaaactc
     9481 tttatagact gggttgatac agaatggtgt tggcgtgcat tagctaataa tatgattatt
     9541 gttcagacac catcagtcat catttctcat gaacttgggt atgggcagaa aatttttgct
     9601 ggtcgatctg ttacaataca taattctttc agaaattttt ataaaatacg caatgcaata
     9661 tacttaatgc tgcattcaaa ttatagcttc aagtatcgtt atcatgcttt ttttcatgcg
     9721 acaaagaatg ttgtatttga aattttatat tcgaaagaaa aattaaattc actgaaggtt
     9781 tgttttaaag ctgtacgtga tggtatgttc aataattttt aatacgaaaa tagttaggct
     9841 caaggtgttt aaatggaaga aaataatatg aagacggtcg ctgtagttgg cacagtgggt
     9901 gttcctgctt gttatggtgg gttcgaatca cttgttcaga atctaattga ttatcaatct
     9961 gatggtatac aatatcagat attttgctct tcaaaaaaat atgataaaaa atttaaaaat
    10021 tataaaaatg cagaattaat ctatttgccg ataaatgcca atggcgtctc tagcataatt
    10081 tatgatatta tgtgtttaat tatttgttta ttcaaaaggc cagatgttgt tttaatattg
    10141 ggggtgtctg gttgtttatt tctaccaatt tataaactat tttcaaaatc aaagattatt
    10201 gtcaatattg atgggcttga atggcgtaga aataaatggg gaacgtttgc taagaaattt
    10261 cttaaaatat ctgaggcgat atctattaga atagctgata ttatcatttc agataatcaa
    10321 gcaatagctg attatgtgga aaataagtac aagaaaaaaa gtgtagttat agcttatggc
    10381 ggagatcatg ccactaatct tagtacaccg atagacaatg atcaaaaaaa agaaggttat
    10441 tatttggggc tttgtaggat agagcctgag aataatatag aaatgattct gaatgccttc
    10501 attaatacag ataaaaaaat taaatttatg ggtaattggg ataacagcga gtatggacgc
    10561 cagctaaaaa aatattattc aaactatcca aatatcaccc tactagaacc taactataat
    10621 attgaagagc tttataaact aagaaaaaat tgtcttgcat acattcatgg acactcggct
    10681 ggtggaacaa acccttcttt agttgaagcg atgcatttta atattcctat ttttgctttc
    10741 gattgtgact ttaatcgtta cacaactaac aatttagctc attactttaa tgattctgaa
    10801 caacttagct tattagcaga aagtttgtct tttggaaatc ttaaatgtcg agtattagat
    10861 ttaaaaaatt atgctgaaga tatgtataac tggaggcata tagctgctat gtatgaatct
    10921 atttattaaa cgcattaaca ataatataat tgaccttata tagcagggaa agatcacgta
    10981 acgctgcttt tttgtactaa ataattcgca ttttatgttt aaaaattgag atatccctta
    11041 ttacctaaag ctgtttttta ttgcttatac atgatcaaat actccttaca taattaagga
    11101 gaacaaaatg gaacttaaaa aattgatgga acatatttct attatccccg attacagaca
    11161 agcctggaaa gtggaacata aattatcgga tattctactg ttgactattt gtgccgttat
    11221 ttcgggtgca gaaggctggg aagatataga ggattttggg gaaacacatc tcgatttttt
    11281 gaagcaatat ggtgattttg ataatggtat tcctgttcac gatactattg ccagagttgt
    11341 atcctgtatc agtcctgcaa agtttcacga gtgctttatt aactggatgc gtgactgcca
    11401 ttcttcagat gataaagacg tcatcgcaat tgatggaaaa acactccggc attcttatga
    11461 caagagtcgc cgcaagggag cgattcatgt cattagtgcg ttctcaacaa tgcacagtct
    11521 ggtcatcgga cagatcagga cggatgagaa atccaatgaa attacagcta tccctgaact
    11581 tcttaacatg ctggatatta aaggaaaaat catcacaact gatgcgatgg ggtgccagaa
    11641 agatattgca gagaagatat aaaaacaggg cggtgattat ttattcgctg taaaaggaaa
    11701 tcaggggcgg cttaataaag cctttgagga aaaatttcca ctgaaagaat taaataatcc
    11761 agaacatgac agttacgcca ttagtgaaaa gagtcacggc agagaagaaa tccgtcttca
    11821 tattgtttgc gatgtccctg atgaacttat tgatttcaca tttgaatgga aaggactgaa
    11881 gaaattatac gttgcagtct cctttcggtc cataatagca gaacaaaaga aagagccaga
    11941 aatgacggtc agatattata tcagttctgc tgatttaacc gcagagaagt tcgccacagc
    12001 aatccgaaac cactggcacg tggagaataa gctgcactgg cgtctggacg tggtaatgaa
    12061 tgaagacgac tgcaaaataa gaagaggaaa cgccgcagaa ttattttcag ggatacggca
    12121 catcgctatt aatattttaa cgaatgataa ggtattcaag gcagggttaa gacgtaagat
    12181 gcgaaaagca gccatggaca gaaactatct cgcgtcagtc cttgcgggga gcgggctttc
    12241 gtaatcttgc cctgccttat atagctatca gagtaaaaaa tgtaacgctt attattttgt
    12301 taaataatac gcattatata ttcaataatt aaaatgctct taattaaata aaactattta
    12361 attaataata taaaatttca ttatgttact taagagatga aaattataac ttaaaaaagt
    12421 gatggaacat atttctatta tccctgatta cagacaagcc tggaaagtgg aacataaatt
    12481 gtcggatatt ctactgttga ctatttgtgc cgttattttt ggtgcagaag gttgggaaat
    12541 atagaggatt ttggggaaac acatctcgtt tttttgaagc aatatggtga ttttgaacat
    12601 ggtattcctg ctcacgatac cattgccaga gttgtatcct gtatcagtcc tgcaaaattt
    12661 cacgagtgct ttattaactg gatgcgtgat tgttattctt catatgataa agacgtcatc
    12721 gcaattgatg gaaaaacact ccggcattct tatgacaaga gtcgccgcaa gggagcgatt
    12781 catgtcatta gtgcgttctc aacaatgcac agtctggtca tcggacagat caagacggat
    12841 gagaaattca atgaggtcac agctatccca gaacttctta acatatggat attaaaggaa
    12901 aaattatcac aactgatgcg atgggttgcc agaaagatat tgcagagaag atacaaaaac
    12961 gggggaggtg attatttatt cgctgtaaaa gtaaattagg ggcggcttaa taaagccatt
    13021 gagggataat ccccgctgaa agaattaaat aatccaaagc atggcagtta cgaaataagt
    13081 gaacagagtc acggcagaga agacatccgt cttcatattg tttgcgatgt tcctgataaa
    13141 cttattgatt tcacgtttta atggaaagga ctgataaaat tatgcgtggc agtctccttt
    13201 cggtcaataa tagcagaaca aaagaaggag cctgaaatga cggtcagata ttatatcagt
    13261 tctgcgggtt taacagcaga gaagtttgcg acagtgattc gaaatcactg gcacgtgaaa
    13321 aataagctgc actggcgtca gggcgtggta atgaatgaag accactgcaa aataagaaga
    13381 ggaattgcag cagaattatt ttcagggatc aggtatatcg gcattaatat tttgacgaaa
    13441 cataaagtat tcaaggctgg gttaagatgt aagatgagaa gagcagatat ggacagagac
    13501 tacctcgcat cagtttttgc ggggagctgg ctttcgtaat cttgccctgg tgatgcaatg
    13561 ctgaattaat agcattaaag attttaaata aaatggttta actcaacatg aactttataa
    13621 ttattattag aatgattttg cctttctgta ttttttgagt attttacttt actctggaag
    13681 ggtggatagt gtttttttat tttctattga atacacattt ataagtgtgg caatctttct
    13741 gataaagaga aagtttgtaa gtaatatttt atgggtaatt tatctttcca ttttaggtgt
    13801 gcaggcagcg tcgctattta gttcaggaca atatttgatg cctcttgcct tgtcgaatgc
    13861 tgcagaaatt gaatccttgg gtattggtga ggtaattaaa gttttttttg tttttatttt
    13921 gtttattata atttcattca ttaccttgcc ttcaggggct ctaaccgagc gtattaaata
    13981 ttataaattc aaccttctct gtatagtatt attgctcgtg tgttctttca taagtgcggg
    14041 accagtatat gctttttatg aaacagcaaa attgttctat ttacagcaaa cctttaagcc
    14101 tagtaagaag atcggagatg cagggaaatt ctttttaaaa acaagtgttt ttattaaccc
    14161 tatagaaaac aactacagtt tcaaaggccg taatgttatt gtaatatttg ctgagggatt
    14221 ttcatctgaa attatgggta atgccaaacc aagagcattt tcagttacgc ctaatttaga
    14281 taaattatct actgaagcac taagtttcaa aaattattat aaccatacag ctgcaacatt
    14341 ccgaggatta agaggtcagt taacttctgg ttatcaattt cgagatggtt taactgacgc
    14401 tggcacaggt atcgcacaat tatctaaagc agaaattaat aatatttacc ttcaccgtca
    14461 aacatcttta cctgatatat taaaagagta tggatataaa agttatttta tagcttccac
    14521 cgaaaaaaat agcccactca atacaatgtt gaagacactt aattttgata aggttcttgg
    14581 tatgggtgat tttattggtt atcaacgaga cagaatgacg gataagcaaa catttaatgc
    14641 actgcaatat tttttacgtt ctcaggaaaa taaaaaagga cccttcttta ttggtgtcta
    14701 tccatctggt actcatcatg gtcaagatag cccaaatgaa aaatattttg atggtaacaa
    14761 ccctctctac aataagttct ataactatga ttttcagtta gggaaattta ttgacttctt
    14821 tcggcgcagt tcattttata ataatacatt attaataata acgtcagatc attcaacatt
    14881 tccttcgaca gaatataaaa aagcatttaa tagcgattct cgttactttg taggcgaaat
    14941 accatttctg gttattggag gagatattac tcctgaaatt ttggatgcag acggtaaaaa
    15001 ttcattaagc tttgcgccaa caatattgca tatgttaggg attcagtatt caatgaacta
    15061 ttttttggga tgttctctta ttgataagga atgcacatcg ccattttcac atctttctgc
    15121 tattggtagt gactatttcg ttacaagcaa agaaaatcat gttgaactaa taccaaacaa
    15181 taatgatagt gaattaataa agcttgcaga aagtttctat aatatcagtg gataagcaga
    15241 tggttgggca aaaaataatc gcctcatcta attatgaaca tacgactgta aaataagata
    15301 taaattaagc tattctttat cttaaataag ccgcaacccc gcggtgacca cccctgacag
    15361 gagtaaacaa tgtccaagca acagatcggc gtag
//
