LOCUS       DQ851855                8132 bp    DNA     linear   BCT 01-AUG-2007
DEFINITION  Escherichia coli strain O22 O antigen gene cluster, partial
            sequence.
ACCESSION   DQ851855
VERSION     DQ851855.1  GI:113367011
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Liu,Y., Fratamico,P. and DebBroy,C.
  TITLE     Identifying Escherichia coli O22 and O91 serogroups by multiplex
            PCR of wzx and wzy gene of O-antigen gene cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Liu,Y., Fratamico,P. and DebBroy,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2006) ERRC-USDA, 600 E. Mermaid Lane, Wyndmoor,
            PA 19038, USA
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="O22"
                     /db_xref="taxon:562"
     misc_feature    <1..>8132
                     /note="O antigen gene cluster"
     gene            56..1147
                     /gene="wbhP"
     CDS             56..1147
                     /gene="wbwZ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABI34559.1"
                     /db_xref="GI:113367012"
                     /translation="MKVLFLITRGDELGGAQTHVKDVILGLINKYNIECYLACGTKGI
                     FTDIMEENNINVIHIDSMKREICFGDIIALKKLNDIIKDINPDIISCHSSKAGVLGRL
                     ASLGTRTKKVFTAHGWAFTEGISPKKAAIYKKIELLLSYITDATINVSYYDKKLALKA
                     GLKSQHYVIHNCIPDVHYEKNNGIANKTVLEFIMVARFCAQKDHETLLKAFSNIDKEK
                     WRLTLIGGGDSKSIKELAKKLNIDNNINFVGQTKNVVDFLNHSDVFLLISNWEGFPIS
                     ILEAMRSSLPIIATDVGGVSEAVKHGYNGFLINKGNVAELEETIIKIIESYSSLKILS
                     QNSRKTYCERFKVDKMIDLYASIFDEVLK"
     gene            1144..1932
                     /gene="wbhQ"
     CDS             1144..1932
                     /gene="wfbH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABI34560.1"
                     /db_xref="GI:113367013"
                     /translation="MKKTIILIPVYNDKGDLLKTLNSINEDDDNFEILIIDDGSVIPL
                     TLPREKYKFKINIFRLSENMGITKALNTGLDIISNRKDIDYIARLDAGDIQQPNRITI
                     QKKTFESDSELVMLGTNVKFYDTNHNYLFDTKLPLTDIQIKKEKYIRSCFIHPSVMFT
                     KRIIDQGMRYSTKFPAAEDYEFFLRIILNYKVANLDKSYVYCFDRATGISIRKRNIQI
                     NSVLKCLFKHRDYTSTYWYFGVFKVLMQKIFPRKVFNLIKSKQK"
     gene            1929..2798
                     /gene="wbhR"
     CDS             1929..2798
                     /gene="wbhR"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABI34561.1"
                     /db_xref="GI:113367014"
                     /translation="MIKTTVIIPYKNNPDALKRALVSVSSQTVPVSIIVIDDNSEKWC
                     SAEKIVQEQNKFSDITIITHKNNQGGGAARNSGIIKAKTQYIAFLDCDDYWDEDKIEK
                     QEVLFENIKNEKAIIFSSIKVVNENLDVIKLYDNDNIIVNNFASYVFKDSGLIQTSSI
                     FLDTNFAKKNLFDASLKRHQDYDFCLKLQKDGGIFYGCGGTFSYWVVPQDPLIALKKG
                     YDTNLSIDFYQKYKTLMGEKTSCYFLVKTPMWFSLKQRKLFSFLLNLSRKCGVKTTLR
                     VLMTAFELAFKRV"
     gene            2799..4034
                     /gene="wzx"
     CDS             2799..4034
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ABI34562.1"
                     /db_xref="GI:113367015"
                     /translation="MNNNIIDTAGRLKLIAFNFMIFVFLVRVPGFPFIILSIISITIL
                     SLFLIKVKVALNNKLIPLYCFVIYMILRCVIGFVFNGGYEERYFMVLLLMLFVLIVFT
                     VELKDVRLEQCYNGISAAVIVTLLIVLYEIISGNHMSTSRYSDINNEAFLWSVSKPTA
                     FYYNENDMLSFVVCFLPFTFVLKNTMLRVAIFLLAAASAIYIGSKAAIITILIYGLFT
                     KIISKKAILLLVVTGVVLVNLFATSLIENTSSFISGSSERLLGFWDTILSTRGGDEST
                     SERLSIYKANLDWLGNHPLSATFGNGRFGDYESELMKVYGLRMGEFHNLHLEMITIFG
                     LLFYLLFFSYYFYLWICLFLKKNKNTACYPLLISSAMYPLIISFGPSSSLKYPFLFIL
                     CLLMILGFRNDEKHAKKNI"
     gene            4003..4587
                     /gene="wbhS"
     CDS             4003..4587
                     /gene="wbhS"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="ABI34563.1"
                     /db_xref="GI:113367016"
                     /translation="MMKSTLKKIYRTLLLIQMSPFLVWALLKNKLPYSNYKNITIAMS
                     KFTGEKGVILRQYFYKLTLDSCGHNLRVHYGAFFVYPNVKVGNNCTVEEYSIVSLCDL
                     GDDVIIAARVSIMSGGNHHEIDKLDIKFVDSLLPLKKVKIGSNVWIGTHAIVMNDIPD
                     GMVVGAGSVVTKVFQPDSIIAGIPAKVMRKRGKS"
     gene            4626..5744
                     /gene="wbhT"
     CDS             4626..5744
                     /gene="wbhT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABI34564.1"
                     /db_xref="GI:113367017"
                     /translation="MRTQGLFFRDQARALSKEHEVDVLYCHRGKRFYSNLYTDDGIKT
                     FYWGYRLRIGSILGMISRIFIYCMMFILYEKKRKVDVIHCHSVAFNQDGSAGIAGVIL
                     GKLFSIPVVITEHATVFSNKNYGRFEKRLMRWALSNADMIICVSEGLRDILEEMTLRQ
                     DILIIPNTIDFKTFVNNDIVVHKIKKENDKIKICSVGYLMEKKGFDRLINVINKLIKD
                     FGYDIELSIVGAGPQYDSLSILIRQYDLQDSIFLLGELDKKQISELMLSSDLFILLSR
                     VETFGVVIIEGLATGLYCVATKCGGPEYIIKDKKLGHLLKNTNDVEYLSSEIDNLLQN
                     KTYDKDQIYRMNYARQEYGFERFLELFNDLVLKKYENK"
     gene            5731..6948
                     /gene="wzy"
     CDS             5731..6948
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ABI34565.1"
                     /db_xref="GI:113367018"
                     /translation="MKINDLLKKYTLFSLSPLLSAFIQLASIFLLGHSLSGPELGIAG
                     IYNTIVFILVFISDGGSSSYFIYRKFLPKYDVNLINIFNVAISAFAIIIIGYYVPEKS
                     DLLGLIVVSLTVTLPLYNYARLLVEKKYNIIAIVELKSKLLFFITLYIAINEFDYQGS
                     AVVMSWAVSYFLRYCLFWWFSKKIELYKNDNLPQNKPAAILKSWWMYIHNQILSQVLN
                     YFTITFDIFIISHFGGLEKVGVYSLCKDATLKISAVLSPVISKLLISHVVNIEENKIS
                     PMYKKVYKATIIISISVFGIWALCGSSIIHFIRPQLDQGILTFIIGWSICGILRMMIN
                     PIVSIFQATGKTRNELYVNITSAVSFIFFMGMLSIYFDNIADAVILALICMYSISFVY
                     ATVLLRKYFSKIY"
     gene            6959..7975
                     /gene="galE"
     CDS             6959..7975
                     /gene="galE"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose C4-epimerase"
                     /protein_id="ABI34566.1"
                     /db_xref="GI:113367019"
                     /translation="MKILITGGTGYIGSHTILMLLNEGYDIIVIDNFSNSSPKVIERI
                     EFLSQKKVCFHEGDIRDERFLKSVFKEHVITTVIHFASLKSVSESISMPIEYYDNNIS
                     GTLTLLKVMNYFGVNNFIFSSSATVYGAPSRIPLDESCDTGDTTNPYGTSKYFLEQIL
                     IDTQRANPALNIMLLRYFNPVGAHSSGFIGEDPNGIPNNLFPYITQVAIGRLPRLSIF
                     GDDYPTEDGTGVRDYIHVMDLASGHVAALNRQHVLTGLRCYNLGTGKGYSVKEIVETF
                     QKITGKKIPYIVSARRPGDIAECWSSPELAQKELHWEAKRSLEDMVIDAWNWQNKNPN
                     GYLE"
BASE COUNT     2757 a   1056 c   1500 g   2819 t
ORIGIN
        1 tgattggtag ctgtaagcca agggcggtag cgttcttatc tatatggaat aaaagatgaa
       61 agtattattt ttaattacaa gaggagatga gttaggtggc gcacagactc atgtcaaaga
      121 tgttatatta ggattaataa ataagtacaa catagaatgt tatctcgcct gtggtacaaa
      181 aggcatattc acagacatta tggaagaaaa taatattaat gtaatacata ttgattcgat
      241 gaaacgggaa atatgctttg gtgatatcat tgcactaaag aaattaaatg atattattaa
      301 agatattaat cctgatatta ttagttgtca ttcatcgaag gcaggtgtat taggtcgttt
      361 ggcatctttg ggaacccgaa ccaaaaaagt tttcactgca catggttggg cttttacgga
      421 aggaatatct cctaagaaag ctgcaatata taaaaaaatt gagttacttt tatcttacat
      481 aacagatgca acaattaacg tttcatatta tgacaagaaa ttagctctta aggcagggtt
      541 gaaatcgcag cactatgtga tacataattg tattcctgat gtccattatg aaaaaaacaa
      601 tggtattgcc aataaaacag ttcttgaatt tattatggtt gcacgttttt gtgcgcaaaa
      661 agatcatgaa actttactta aagccttttc aaatatagat aaagaaaagt ggcgattgac
      721 tttgattggt ggtggagata gtaagtcaat taaggagtta gcgaaaaaac ttaatataga
      781 taacaatatt aattttgtag gtcaaacaaa aaatgttgtc gactttttga accattctga
      841 tgtttttctt cttatatcta actgggaagg cttccctatt tctatactag aagcaatgag
      901 aagtagttta cccatcatag ccactgacgt gggaggagtt agcgaagctg tcaagcatgg
      961 ttacaatggc tttttgatta ataaaggaaa tgtagctgaa ctcgaagaaa caataattaa
     1021 aataattgaa tcttattcat cattaaaaat acttagtcaa aattcaagaa aaacatattg
     1081 tgaaagattc aaagttgaca agatgataga tttatatgca tcaatattcg atgaggtatt
     1141 aaaatgaaaa aaacgattat cttaatacct gtctataatg ataaaggtga tttactgaaa
     1201 acattaaata gcatcaatga agatgatgat aatttcgaaa tattaattat cgatgacgga
     1261 agtgtcatac cattaacatt acctcgcgaa aaatataaat ttaaaattaa tattttcagg
     1321 cttagtgaga atatggggat aacaaaagcg ctaaatacag ggttagacat aataagcaat
     1381 agaaaagata tagattatat agcgcggtta gatgcgggag atatacagca accaaatcga
     1441 ataacaattc agaagaaaac atttgaatcc gatagtgaat tagtgatgct tgggacgaat
     1501 gttaaatttt acgacacaaa tcacaattat ttatttgata ctaaattgcc tttgacagat
     1561 atacagataa aaaaagaaaa atatataaga tcttgtttta tacatccttc ggtaatgttt
     1621 actaaaagga ttattgatca aggaatgcga tattcgacaa aatttcctgc agcagaagat
     1681 tatgaatttt ttcttagaat tatattaaat tataaagtgg ctaatttaga taagtcatat
     1741 gtgtattgct ttgatcgggc taccgggatc agtatcagaa agcgaaatat ccaaatcaat
     1801 agtgtcttaa aatgcttgtt taagcatcga gattatacat caacatattg gtatttcggt
     1861 gttttcaaag tgttaatgca aaaaattttc ccgagaaaag tgtttaattt aattaagtcg
     1921 aaacaaaaat gattaagaca acagtaataa ttccctataa aaataatcca gatgctctca
     1981 aacgagcact ggttagcgtg agtagccaaa ctgtacctgt tagtattatt gtcatcgatg
     2041 ataattcaga aaaatggtgt tcggcggaga aaatagttca agaacaaaat aaattctccg
     2101 atattacgat aatcacgcat aaaaacaatc aagggggggg cgctgctagg aatagtggca
     2161 ttatcaaagc aaaaacacaa tatatcgctt ttttggattg tgatgattac tgggatgaag
     2221 ataaaattga aaagcaagaa gtactattcg aaaacataaa aaatgaaaaa gcaataattt
     2281 tttcatcaat aaaagtagtt aatgaaaatt tagatgtgat taaattgtat gataatgaca
     2341 atattattgt aaataatttt gccagctatg tatttaaaga tagtgggtta atacagacat
     2401 catcaatttt tttggataca aattttgcta aaaaaaactt atttgatgca agtttaaaaa
     2461 gacatcagga ctatgatttt tgtttaaagc tacaaaaaga tggtgggatt ttttatgggt
     2521 gtgggggaac ttttagttat tgggtcgtgc ctcaagatcc attgattgca ctaaaaaaag
     2581 gatacgatac aaatttaagt attgattttt atcaaaaata taaaacgtta atgggggaaa
     2641 aaactagttg ctattttcta gtaaaaacgc ctatgtggtt cagtttgaaa caacgtaagc
     2701 tgttttcttt cttactaaac ttgtctagaa agtgcggagt aaaaacaact ctccgagttt
     2761 taatgactgc atttgagctg gctttcaaaa gggtctaaat gaataacaac attatagata
     2821 ctgcaggtag attaaaattg attgctttca attttatgat ttttgttttt ttggttagag
     2881 ttcctggatt tccctttata attttatcta ttatcagtat aacgatacta tccttatttt
     2941 taataaaagt taaggttgca ttaaataaca agctaattcc tctttattgt tttgtgatat
     3001 atatgatcct tcgttgcgtt ataggttttg tatttaatgg gggatatgaa gaacgatatt
     3061 ttatggtttt attgttaatg ctatttgttc taattgtatt tactgtagaa ttgaaagatg
     3121 taagattaga acagtgttat aatgggatat cagctgcagt aattgttacc ttgctaattg
     3181 tgttatacga aataattagt ggtaaccata tgagtacatc acgttattca gatataaaca
     3241 atgaggcatt tttatggagt gtttcaaaac caacagcctt ctattataat gaaaatgata
     3301 tgttgagctt tgttgtttgc tttttaccgt ttacttttgt attaaagaac actatgttgc
     3361 gtgtcgcaat atttttactg gctgctgcta gtgctatata tataggttcg aaagctgcaa
     3421 taataacgat tctaatttat gggttattta caaaaataat ctctaaaaag gcgatattac
     3481 ttttagtggt aactggtgtt gttcttgtca atttatttgc aacatcacta attgaaaata
     3541 ccagttcctt tatatccggc tcttcagaaa gattacttgg tttttgggat acgatacttt
     3601 cgactcgtgg tggagatgaa agtacctcag aacgtctttc catttacaaa gctaatctgg
     3661 attggcttgg taaccatcct ttgtcagcta catttgggaa cggaaggttt ggtgattatg
     3721 aaagcgaatt gatgaaagtt tatggtttaa gaatggggga atttcataat ttacatcttg
     3781 aaatgataac tatattcggt ttgttatttt atttgttatt ttttagctac tacttttatt
     3841 tatggatttg tttatttctt aaaaagaaca aaaatacagc ctgttatcct ttgttaatat
     3901 catcagcaat gtatccacta ataatctctt ttgggccaag tagttctttg aagtatccat
     3961 ttctttttat tttatgttta ctaatgatat taggatttcg aaatgatgaa aagcacgcta
     4021 aaaaaaatat atagaacatt gttattaatt caaatgtctc cgtttttagt gtgggcgtta
     4081 ttaaaaaata aattgcctta ctcaaactat aaaaatataa ctattgctat gtctaagttc
     4141 acgggagaaa aaggagttat actaaggcag tatttttata aactcacctt agattcctgt
     4201 gggcataatt taagagtgca ttatggggct ttttttgttt atccaaacgt taaggttggt
     4261 aataattgca ctgtagagga atactctatt gtcagcttat gtgatctagg tgatgatgtt
     4321 attatcgcag caagagtatc gattatgagc ggtgggaatc atcacgaaat agataagttg
     4381 gatataaagt ttgttgactc acttcttcct ttaaaaaaag ttaaaattgg ttccaatgtt
     4441 tggattggaa cccatgctat tgtcatgaat gatattccag atggtatggt cgttggtgct
     4501 gggagtgttg ttacaaaagt atttcaacct gattcaatta ttgcgggtat tcctgcaaag
     4561 gttatgagaa aaagagggaa atcgtgaaaa ttacagttat tccatcttgg tatccgaatc
     4621 ctaaaatgag aacacaagga ctttttttta gagaccaagc tagagcactt tcaaaagagc
     4681 atgaagttga tgttttatat tgccatagag gaaaaagatt ttattcaaat ttatataccg
     4741 atgatggcat taaaactttt tactggggat atagactgcg cataggaagc attttaggaa
     4801 tgatatctag aatcttcata tattgcatga tgtttatcct ctatgaaaag aaaagaaaag
     4861 tagatgttat acattgtcat agtgttgctt tcaatcaaga tggcagtgcc ggtattgctg
     4921 gtgtgatttt aggtaaatta ttttctattc ctgttgttat aacagaacat gcaacggttt
     4981 tttcaaataa aaattatggt aggtttgaga aaagattaat gcgatgggct ttatctaatg
     5041 ctgatatgat aatttgtgtt agtgaaggct tgcgtgacat tttagaagaa atgaccctaa
     5101 gacaggatat actaataata ccaaacacaa ttgattttaa aacttttgtg aataatgata
     5161 ttgtcgtaca taaaattaaa aaagaaaacg acaaaatcaa aatatgttca gtaggttatc
     5221 taatggagaa aaagggcttt gatcgactta tcaatgtgat aaataaattg attaaagatt
     5281 ttggttatga tatagagttg agtattgttg gtgcgggtcc acaatatgat tcgctatcaa
     5341 ttctcattcg acaatatgac ttacaagatt caatatttct actgggggaa ctcgataaaa
     5401 agcaaatatc tgaattgatg ctctcaagcg atttgtttat tttgctaagt cgagtagaaa
     5461 cttttggggt tgttattatt gaggggttag caaccggatt atattgcgtt gcaactaaat
     5521 gtggcgggcc agaatatata attaaagata aaaaactagg tcatttactc aaaaatacta
     5581 atgatgttga atatctaagt tcggaaatag ataatctttt acaaaataaa acttatgaca
     5641 aggatcagat ttatagaatg aattatgctc gtcaagaata tggattcgaa cgtttcttgg
     5701 aattatttaa tgatctggtt ttgaaaaaat atgaaaataa atgatttatt gaaaaaatac
     5761 acactttttt cattgtcgcc actactttcc gcgtttattc agctagcttc tatttttctt
     5821 ttaggtcatt cattatcagg tccggaacta ggtatagcag gaatttataa cacaatagtt
     5881 tttattttag tttttatttc ggatggcggt agttcaagtt attttattta tcgtaagttt
     5941 ttgccaaaat atgatgttaa cttgatcaat atttttaatg tggccatatc tgcattcgct
     6001 ataattatca taggttatta cgttccagaa aaatccgatt tattgggatt aattgtagta
     6061 tcactaacag ttacgcttcc attatataat tatgctcgtt tgttagtaga aaagaaatac
     6121 aatattatcg ctatagttga actaaagtca aaattgttat tttttattac attatacata
     6181 gcaattaacg agtttgacta tcaaggcagt gccgtagtaa tgtcatgggc tgtttcttat
     6241 tttttaagat attgtttatt ttggtggttt tctaaaaaaa tagaattata taaaaatgat
     6301 aatttacccc aaaataaacc agcggcgatt ttaaaaagtt ggtggatgta cattcataat
     6361 caaattttgt cacaagtact aaattatttt acaattacat ttgatatttt tattatatcg
     6421 catttcgggg gacttgaaaa agtaggtgtg tacagtttgt gcaaagatgc cactttaaaa
     6481 ataagtgctg ttttaagccc agtaataagc aagttgttaa tctctcacgt tgttaatatt
     6541 gaagagaata aaatatcgcc tatgtataaa aaagtataca aggcaactat tataatctct
     6601 ataagtgtat ttggcatttg ggctttatgc ggaagtagta ttattcactt tattagaccc
     6661 caactggatc aaggtatttt gacctttata atcggatggt ctatatgtgg cattcttaga
     6721 atgatgatta atccaattgt ttctattttt caggcgacag gaaaaacaag aaatgaatta
     6781 tatgttaata ttacttcggc ggtctccttt attttcttta tgggaatgct atcaatatat
     6841 tttgataata tagccgatgc tgttattctt gctttaatat gtatgtattc tatttcgttc
     6901 gtttatgcta ctgttttatt gagaaaatat ttttctaaaa tttattaagg gtgggtttat
     6961 gaaaatatta ataactggtg ggactggtta tataggttcc catacaattt taatgttgct
     7021 aaatgaaggt tatgatataa ttgttataga taatttttct aattcatcgc ctaaagttat
     7081 agaaagaatt gagtttcttt ctcaaaaaaa agtatgcttt catgaagggg atataagaga
     7141 tgaaagattt ttgaaatctg tttttaagga acatgtcatt acgacggtaa ttcattttgc
     7201 ttcacttaaa tctgtttctg aatctatttc aatgcccatt gaatattatg ataataatat
     7261 ttctggaact ctgaccttgt tgaaggtaat gaattatttt ggtgttaata attttatctt
     7321 tagttcatct gcaacagttt atggtgctcc ttctagaatt ccgcttgatg agtcgtgtga
     7381 tactggagat actacaaatc catatggaac ttcaaaatat tttttggagc agatattgat
     7441 tgatacacaa cgtgctaatc ctgcattaaa tattatgctg ctgaggtatt ttaatcctgt
     7501 cggagcccat tcatcaggtt tcatcggcga ggacccgaat ggaattccaa ataatctatt
     7561 tccatatatt acccaagtgg ccattggaag attaccgagg ttgtcaatat ttggagatga
     7621 ttatccaacc gaggatggta ctggagtaag agattatata catgttatgg atttagcatc
     7681 tggtcatgtt gctgctctca atagacagca tgttttaacg ggattgcggt gttacaatct
     7741 tggcacaggg aaaggttatt cagtcaaaga gattgttgaa acttttcaaa agataactgg
     7801 aaaaaaaata ccatatattg tttctgcacg tcgtcccggt gatatagcag aatgttggtc
     7861 ctcaccagag ttagctcaaa aagaacttca ttgggaagct aagcgtagtc ttgaggatat
     7921 ggttattgat gcttggaact ggcaaaataa gaatccaaat ggatatttag aatgatgtta
     7981 tattatttct tttaaaggca gtttgctact atcaatattt tgtttgaaat agtctaatat
     8041 atgattccaa aaccgcatac atcgcggtga ccaccccctg acaggagtaa acaatgtcca
     8101 agcaacagat cggcgtcgtc ggtatggcag tg
//
