LOCUS       AF529080               13270 bp    DNA     linear   BCT 29-OCT-2002
DEFINITION  Escherichia coli serotype O26:K60:H311b O-antigen gene cluster,
            partial sequence.
ACCESSION   AF529080
VERSION     AF529080.1  GI:24417728
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13270)
  AUTHORS   D'Souza,J.M., Wang,L. and Reeves,P.
  TITLE     Sequence of the Escherichia coli O26 O antigen gene cluster and
            identification of O26 specific genes
  JOURNAL   Gene 297 (1-2), 123-127 (2002)
   PUBMED   12384293
REFERENCE   2  (bases 1 to 13270)
  AUTHORS   D'Souza,J.M., Wang,L. and Reeves,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2002) Microbiology, University of Sydney, School
            of Molecular and Microbial Biosciences, Sydney, NSW 2006, Australia
FEATURES             Location/Qualifiers
     source          1..13270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /serotype="O26:K60:H311b"
                     /db_xref="taxon:562"
     gene            161..1054
                     /gene="galF"
     CDS             161..1054
                     /gene="galF"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate uridylyltransferase"
                     /protein_id="AAN60453.1"
                     /db_xref="GI:24417729"
                     /translation="MMNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGELSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSK"
     gene            1426..2511
                     /gene="rmlB"
     CDS             1426..2511
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AAN60454.1"
                     /db_xref="GI:24417730"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEQSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVENVKSGAYQSWIAQNYEGRQ"
     gene            2511..3410
                     /gene="rmlD"
     CDS             2511..3410
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /protein_id="AAN60455.1"
                     /db_xref="GI:24417731"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWLIHY
                     STDYVFPGNGDMPWRETDATAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQKFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3468..4346
                     /gene="rmlA"
     CDS             3468..4346
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AAN60456.1"
                     /db_xref="GI:24417732"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGLRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNYVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            4351..4893
                     /gene="rmlC"
     CDS             4351..4893
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /protein_id="AAN60457.1"
                     /db_xref="GI:24417733"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSEIAEFVYKTTNYYHPESEGCIKWDDSFLMIDWPNK
                     PIAISEKDKKGSSILRLNDY"
     gene            4893..6155
                     /gene="wzx"
     CDS             4893..6155
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAN60458.1"
                     /db_xref="GI:24417734"
                     /translation="MLKKKLQKIKEYHSVLELAIIQGANAIFPVLVFPFFLITLGENI
                     FSSIAVGEVLALYVLIFSLYSFDIISVQKVISSVTKDEIFKVYILTLICRLCLFVISG
                     ICLLFITYLINKTLSVYLGLFLLYPVGMILQSNYFFQATNNNRPLAVFVLIARGMSLC
                     LIYFYNGPAGYLTSYYYVICVSGSYFLSGVLSLIYIYYQNKTNKAKIQWAEILEYICT
                     GYHLFIANIFVILYRNSNIIILGTLASPVATSLYATAEKIIKCIQSIATPLNQYYFTR
                     LIKQHELKLEPYKVGEYKSLLYASTNIQLKFMVFIVLSLGGVGTILGYKVQSIAEIRS
                     AFIPLSIMSFAIFMGIYNFMFGSVGLSIRGYKKEFSYIVAITGVSTIILSLCLSYFFA
                     EIGAAIAYVFAEFILLILILRIYKVKRL"
     gene            6221..7243
                     /gene="wzy"
     CDS             6221..7243
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAN60459.1"
                     /db_xref="GI:24417735"
                     /translation="MYYIIFVMVLGLWIIAFNYARANKLSNLVIVLIITTLFIINRQN
                     QDYEAYVDIFNVNELYAEIGYRWLIYGVKYLGGTHEVIIGLLGLFLGTTFLRLIQYSK
                     YTAFGLLLYMLCPMPIDIVQIRNTFLFLFVINSLIELEKEHKFNSLCFVFMAPLFHSL
                     GIVYVLAWVIIQFRTWRGYNKLMVFGLVLSFIVVPLLIKMLILLFNTRTLHAYIADGI
                     KVHSLIIWAGPYLFDLFLLCYFRKKIVITDLHTKQWIDIIFSLMLFLSVFSPLLLYID
                     EINRIFRNALLLKYLVMMSIAQYLSRPTRYILYSYLLLFTFALSIYYTLQIDYDYIVF
                     GLPYSL"
     gene            7259..8053
                     /gene="wbuA"
     CDS             7259..8053
                     /gene="wbuA"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAN60460.1"
                     /db_xref="GI:24417736"
                     /translation="MFYFICVNYNNSDYTEALIKSVINQEKDSFVIVVDNCSNEQELK
                     KLNDIESKYNEKVRLIKSDVNLGYFGGLNLGLKGLPRNHPIVVGNNDLVYESNFTQII
                     SQTTYPDDALVIAPNVITKDGYHQNPHCRKRVSKFRKFLYDIYFSNYIAALVLTWGSR
                     FLNYLKGGRNNSFDQGRGYIHMGIGACYVLMPSFFKYFDELDNKVFLYGEEAYLAGQL
                     MEVNGKIFYEPDAIVHHEESATLAKVASKTKYGYMKSSYYDYKKYL"
     gene            8065..9099
                     /gene="fnl1"
     CDS             8065..9099
                     /gene="fnlA"
                     /note="involved in O-antigen biosynthesis"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlA protein"
                     /protein_id="AAN60461.1"
                     /db_xref="GI:24417737"
                     /translation="MFKNKTLVITGGTGSFGNAVLKRFLDTDITEIRIFSRDEKKQDD
                     MRKKYNNSKLKFYIGDVRDYNSVLNATRGADFLYHAAALKQVPSCEFHPMEAVKTNVL
                     GTENVLEAAIANGIKRVVCLSTDKAVYPINAMGISKAMMEKVIVAKSRNLDSSKTVIC
                     GTRYGNVMASRGSVIPLFVDLIKAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNN
                     GDIFVQKAPAATIQTLAIALKELLNAHEHPINIIGTRHGEKLYEALLSREEMIAAEDM
                     GDYYRVPPDLRDLNYGKYVEHGDRRISEVEDYNSHNTERLDVEGMKELLLKLPFIRAL
                     RSGEDYELDS"
     gene            9083..10207
                     /gene="fnl2"
     CDS             9083..10207
                     /gene="fnlB"
                     /note="involved in O-antigen biosynthesis"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlB protein"
                     /protein_id="AAN60462.1"
                     /db_xref="GI:24417738"
                     /translation="MSWIHNMKILVTGAAGFIGRNLVFRLKEAGYNELIAIDRNSSLT
                     DLEQGLKQADFIFHLAGVNRPVKESEFEEGNCDLTQQIVDILKKNNKDTPIMLSSSIQ
                     AECDNAYGKSKAVAEKIIQQYGETTNAKYYIYRLPNVFGKWCQPNYNSFIATFCHRIA
                     NDEAITINDPSAVVNLVYIDDFCSDILKLLEGANETGYRTFGPIYSVTVGEVAQLIYR
                     FKESRQTLIIEDVGNGFTRALYSTWLSYLSPKQFAYTVPSYSDDRGVFCEVLKTKNAG
                     QFSFFTAHPGITRGGHYHHSKNEKFIIIRGSACFKFENIVTGERYELNVSSDDFKIVE
                     TVPGWTHDITNNGSDELVVMLWANEIFNRSEPDTIARVLS"
     gene            10222..11334
                     /gene="fnl3"
     CDS             10222..11334
                     /gene="fnlC"
                     /note="involved in O-antigen biosynthesis"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlC protein"
                     /protein_id="AAN60463.1"
                     /db_xref="GI:24417739"
                     /translation="MSVVGTRPEIIRLSRVLAKLDEYCDHLIVHTGQNYDYELNEVFF
                     KDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVFEQEKPEAMLVLGDTNSCISAIPAK
                     RRRIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGVPADRII
                     KTGSPMFEVLTHYMSQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAKLANILN
                     TVAEKYDIPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNARAVLSDS
                     GTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESARVLQALEIIATQPRGEVR
                     LLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            11334..12542
                     /gene="wbuB"
     CDS             11334..12542
                     /gene="wbuB"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAN60464.1"
                     /db_xref="GI:24417740"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLSRGHDVTVITPDNT
                     LQAIYSVSMTDGIKVWRFKSGPLKDIGKAKRAINETLLSFRAWHALKHLIQHDTFDGI
                     VYYSPSIFWGGLVKKIKERCQCPSYLVLRDMFPQWVIDAGMIKAGSPIEKYFRYFEKK
                     SYQQADWIGLMSDKNLEIFRQANKGYPCEVLRNWASMTPVSAGDDYHSLRQKYALKDK
                     IIFFYGGNIGHAQDMANLMRLARNMMRHHDAHFLFIGQGDEVDLIKSLSAEWNLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DVINKHRAGFIHVNGEDDKLFESAQLLLSDSVLRKQLGQNANVLLKSQFSVESAAHTI
                     EVRLEAGECV"
     gene            12533..12940
                     /gene="wbuC"
     CDS             12533..12940
                     /gene="wbuC"
                     /note="function unknown"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC protein"
                     /protein_id="AAN60465.1"
                     /db_xref="GI:24417741"
                     /translation="MRLVDDNIMDELFRTAANSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEQNGELQKKFVVGDGTGISVVEFSPGD
                     IHSVKCLSPKALMLEIKEGPFDPLKAKAFSKWL"
     gene            13051..>13270
                     /gene="gnd"
     CDS             13051..>13270
                     /gene="gnd"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="AAN60466.1"
                     /db_xref="GI:24417742"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLM"
BASE COUNT     3887 a   2315 c   2961 g   4107 t
ORIGIN
        1 tttgggcggt gtttgcggag ttcccggcac aaaacatcaa acggcgccgc cagtatcggt
       61 ggtgggagac cccagctcag gcgtatgatg tggcatctgg ccggttgtat ttaggcgttt
      121 gggtctcccc atggcgatgg ttaaaggtat attggacggt atgatgaatt tgaaagcagt
      181 tattcctgta gcgggtcttg ggatgcatat gttgcctgcc actaaggcga ttcccaaaga
      241 gatgctaccg atcgtcgaca agccaatgat tcagtacatt gttgacgaga ttgtggctgc
      301 agggatcaaa gaaatcctcc tggtaactca tgcgtccaag aacgcggtcg aaaaccactt
      361 cgacacctct tatgaattag aatctcttct tgaactgcgc gtgaagcgtc aactgctggc
      421 ggaagtacag tccatctgtc cgccaggcgt gaccattatg aacgtgcgtc agggcgaacc
      481 tttaggttta ggccactcca ttttgtgtgc acgacccgcc attggtgaca atccatttgt
      541 cgtggtactg ccggacgttg tgatcgacga cgccagtgcc gacccgctgc gctacaacct
      601 tgctgccatg attgcgcgtt tcaatgaaac gggccgtagc caggtgctgg caaaacgtat
      661 gccgggtgag ctctctgaat actctgtcat ccagaccaaa gaaccgctgg atcgcgaagg
      721 taaagtcagc cgcattgttg aatttatcga aaaaccggat cagccacaaa cgctggactc
      781 agacatcatg gccgttggtc gctatgtact ttctgccgat atttggccgg aactggaacg
      841 tactcagcct ggtgcatggg gacgtattca actgactgat gctattgccg agctggcgaa
      901 aaaacagtcc gttgatgcca tgctgatgac tggggacagt tacgactgcg gtaaaaaaat
      961 gggctatatg caggcgttcg tgaagtatgg attacgaaac ctgaaagaag gggcgaagtt
     1021 ccgtaaaggc attgagaagc tgttaagcaa ataatgaaaa tctgaccgga tgtaacggtt
     1081 gataagaaaa ttataacggc agtgaagatt cgtgatgaaa gtaatttgtt gcgaatattc
     1141 ctgccgttgt tttatataaa caatcagaat aacaacgagt tagcaatagg attttagtca
     1201 aagttttcca ggattttcct tgtttccaga gcggattggt aagacaatta gcgtttgaat
     1261 ttttcgggtt tagcgcgagt gggtaacgct cgtcacatcg tagacatgca tgcagtgcac
     1321 tggtagctgt aaagccaggg gcggtagcgt gcattaatac ccctattaat caacctaaga
     1381 gccgcttatt tcacagcatg ctctgaagta atatggaata ataaagtgaa gatacttgtt
     1441 actggtggcg caggatttat tggttctgct gtagttcgtc acattataaa taatacgcag
     1501 gatagtgttg ttaatgtcga taaattaacg tacgccggaa acctggaatc acttgctgat
     1561 gtttctgatt ctgaacgcta tgtttttgaa catgcggata tttgcgatgc agctgcaatg
     1621 gcgcggattt ttgctcagca tcagccggat gcagtgatgc acctggctgc tgaaagccat
     1681 gttgaccgtt caattaccgg ccctgcggca tttattgaaa ccaatattgt tggtacttat
     1741 gtccttttgg aagccgctcg caattactgg tctgctcttg atagcgacaa gaaaaatagc
     1801 ttccgttttc atcatatttc tactgacgaa gtctatggtg atttgcctca tcctgacgaa
     1861 gtaaataata cagaagaatt acccttattt actgagacga cagcttacgc gccaagcagt
     1921 ccttattccg catccaaagc atctagcgat catttagtcc gcgcgtggaa acgtacctat
     1981 ggtttaccga ccattgtgac taactgttcg aataactacg gcccttatca ctttccggaa
     2041 aaattgattc cgctggtaat tcttaatgct ctggaaggta aggcattacc tatttatggc
     2101 aaaggggatc aaattcgcga ttggctatat gttgaagatc atgcgcgtgc gttatatacc
     2161 gttgtcaccg aaggtaaagc gggtgaaact tataacattg gcggacacaa cgaaaagaaa
     2221 aacatcgatg ttgtgctgac tatttgtgat ttgttggatg agattgtacc gaaagagcaa
     2281 tcttatcgtg agcaaattac ttatgttgcc gatcgtccgg gacacgatcg ccgttatgcg
     2341 attgatgctg agaagattag ccgcgaattg ggctggaaac cgcaggaaac gtttgagagc
     2401 gggattcgga agacagtgga atggtacctg tccaatacaa aatgggtcga aaatgtgaaa
     2461 agtggtgcct atcagtcatg gattgcacag aactatgagg gccgtcagta atgaatatcc
     2521 tccttttcgg caaaacaggg caggtaggtt gggaactaca gcgtgctctg gcacctctgg
     2581 gtaatttgat tgctcttgat gttcactcta ctgattattg cggtgatttt agtaatcctg
     2641 aaggtgtagc tgaaaccgta agaagcattc ggcctgatat tattgtcaac gcagccgctc
     2701 acaccgcagt agacaaagca gaatcagaac cggagtttgc acaattactt aacgcaacaa
     2761 gtgtcgaagc gattgcgaaa gcagccaatg aagtcggcgc ctggcttatt cattactcga
     2821 ctgattacgt cttccctgga aatggcgata tgccatggcg ggagacggat gcaaccgcac
     2881 cactaaatgt ttacggtgaa accaagttag ccggagaaaa agcgttacag gaatattgcg
     2941 caaagcatct tattttccgg accagctggg tctatgcagg aaaaggaaat aacttcgcca
     3001 aaacgatgtt acgtctggca aaagagcgtg aagaattagc ggttattaat gatcagtttg
     3061 gtgcgccaac aggtgctgaa ctgctggctg attgtacagc acatgccatt cgtgtcgcac
     3121 tgaataaacc agaagtcgca ggcttgtacc atctggtagc cagtggtacc acaacctggc
     3181 acgattatgc tgcgctggtt tttgaagagg cacgcaaagc aggtattccc cttgcactca
     3241 acaagctcaa cgcagtacca acaacagcct atcctacacc agctcgtcgt ccgcataact
     3301 ctcgccttaa tacagaaaaa tttcagcaga aatttgcgct tgttttgcct gattggcagg
     3361 ttggcgtgaa acgaatgctc aacgaattat ttacgactac agcaatttaa tagtttttgc
     3421 atcttgttcg tgatggtgga gcaagatgaa ttaaaaggaa tgatgaaatg aaaacgcgta
     3481 aaggtattat tttagctggt ggttcgggta ctcgtcttta tcctgtaact atggctgtca
     3541 gtaaacagtt gttaccgatt tatgataaac cgatgatcta ttacccgttg tctacactga
     3601 tgttagcggg tcttcgcgat attctgatta ttagtacgcc acaggatact cctcgttttc
     3661 aacaactgct gggtgacggg agccagtggg ggctaaatct tcagtacaaa gtgcaaccga
     3721 gtccagatgg tcttgcgcag gcatttatca tcggtgaaga gtttatcggt ggtgatgatt
     3781 gtgctttggt tctaggtgat aatatctttt acggtcacga tctgccgaag ttaatggatg
     3841 tcgctgttaa caaagaaagt ggtgcaacgg tatttgccta tcacgttaat gatcctgaac
     3901 gctacggtgt cgttgagttt gataaaaacg gtacggcgat cagcctggaa gaaaaaccgc
     3961 tacaaccaaa aagtaattat gcggtaaccg ggctttattt ttatgataac tacgttgtcg
     4021 aaatggcgaa aaatcttaag ccttctgccc gcggtgaact ggaaattacc gatattaacc
     4081 gtatttatat ggaacagggg cgtttatccg ttgccatgat gggacgtggt tatgcatggc
     4141 tggacacggg gacacatcaa agtcttattg aggcaagcaa ttttatcgca acaatagaag
     4201 aacgtcaggg gctgaaagtt tcctgcccgg aagaaattgc ttaccgtaaa gggtttatcg
     4261 atgctgagca ggtgaaagta ttagctgaac cgttgaagaa aaatgcttat ggtcagtatc
     4321 tgctgaaaat gattaaaggt tattaataaa atgaacgtaa ttaaaacaga aattccagat
     4381 gtactgattt ttgaaccgaa agtttttggt gatgagcgtg gtttctttat ggaaagcttt
     4441 aatcagaaag tttttgaaga agctgtaggg cggaaggttg aatttgttca ggataatcat
     4501 tctaaatcat ctaagggtgt gttacgcgga ctgcactatc agctggaacc ctatgctcaa
     4561 ggtaaattag ttcgttgtgt ggtcggtgaa gtatttgatg tagcagttga tattcgtaaa
     4621 tcgtcaccta catttgggaa atgggttggg gtgaatttgt ctgctgagaa taagcgtcag
     4681 ttgtggatcc ctgaggggtt tgcgcatggt tttttggtgt taagcgagat agcagagttt
     4741 gtttataaga cgacaaatta ttatcatcct gaatcagaag gttgcataaa atgggatgat
     4801 tcttttttga tgattgattg gccaaataaa ccgatagcaa tctcagaaaa agataaaaaa
     4861 ggctcatcaa ttttgaggct aaatgactat tgatgttgaa aaaaaaactt caaaaaataa
     4921 aggaatatca ttcagtattg gagttggcaa taattcaggg tgcgaatgcc atatttcctg
     4981 tgttggtatt cccatttttt cttattacct taggggaaaa catcttttca agtattgctg
     5041 ttggtgaagt actagcacta tatgtgctta tattttcgct atacagtttt gatattataa
     5101 gtgtgcagaa ggtaatttca agtgtgacaa aagatgaaat atttaaagtt tacattctga
     5161 cactaatctg taggttgtgt ttatttgtta tttcaggaat atgtctttta tttataacgt
     5221 atttaattaa taaaacatta agtgtatact tgggattgtt tttattgtac ccagtaggga
     5281 tgatattgca atctaattat ttttttcagg ctacgaataa caataggcca ttggctgttt
     5341 ttgtactaat tgctcgtggt atgtcattat gtcttattta tttttataat ggaccagcag
     5401 gctatttaac aagttattat tatgtcattt gtgtgtctgg ttcgtatttt ttatctggcg
     5461 tgctatcgct tatatatata tattatcaaa ataagactaa taaagctaaa attcaatggg
     5521 cggaaatttt agaatatata tgcacaggtt atcatctgtt tattgctaat atatttgtta
     5581 ttctatacag aaatagtaat attattattc ttggcactct tgcttcgcct gttgcaacgt
     5641 ctctgtacgc gacggcagag aaaattatta aatgtattca gtctatagca accccgttaa
     5701 atcaatacta tttcacgagg ttgataaagc aacatgaatt gaaattagaa ccatacaaag
     5761 ttggagaata taaaagcctg ctatatgcaa gcacaaatat tcagctaaag ttcatggttt
     5821 tcattgtcct gagtttaggg ggggtgggta ctatattggg atataaggtt caaagtatcg
     5881 ctgaaattag aagcgcgttc atccctttat caataatgtc ttttgcaata tttatgggga
     5941 tatacaattt tatgtttggt tcggttggat tgtccataag agggtataaa aaagaatttt
     6001 cttatatagt ggccattacg ggtgtttcaa ctattatttt atcattatgc ctgagttatt
     6061 tctttgctga aataggcgct gcaattgctt atgtatttgc tgagtttatc ttacttattc
     6121 tcatacttag aatttataaa gtgaaacgat tataattccc ggtaagaact gatagtgcaa
     6181 aatcatcatg gctatagact gaataaattg cggggaaaga atgtattaca tcatatttgt
     6241 gatggtttta ggtttatgga ttattgcatt caactacgct agagcaaaca agctaagtaa
     6301 tttggtaatt gtgcttatta ttactacttt atttattatt aataggcaga atcaagacta
     6361 tgaagcgtat gttgatatat ttaatgtcaa tgaactttat gccgagattg gttatcgctg
     6421 gttaatttat ggtgttaagt atttaggcgg tacccatgaa gtcataattg gcttgctggg
     6481 tttattcctt gggaccacat tcctacgatt aatacaatac agtaagtata cagcatttgg
     6541 tttgctgctt tacatgttgt gtccaatgcc tattgacatt gttcaaatca ggaatacatt
     6601 tcttttttta tttgtcataa attctcttat cgagttagaa aaagagcata aatttaactc
     6661 tctttgtttt gtctttatgg cacctctttt ccatagttta ggcattgttt atgtattggc
     6721 gtgggtaata atccaatttc gtacctggag gggttataat aagttaatgg tttttggact
     6781 ggtgctgtct tttattgtag tgccactctt gattaaaatg ttaattttat tatttaacac
     6841 taggacgtta catgcatata tagctgatgg cattaaggtt cattctctta ttatatgggc
     6901 aggtccttat ctttttgatc tttttctgtt atgttacttc agaaaaaaaa tagtaataac
     6961 tgatttacat actaaacaat ggattgatat aatattctcg ctaatgttat ttctttccgt
     7021 attttcacca ctacttttat atatcgacga aataaatagg atttttcgaa atgcgctatt
     7081 actgaagtat ctggtaatga tgtctatcgc acagtattta agtagaccga ccagatatat
     7141 actttattca tatttactgt tattcacttt tgcattatca atatattata cactccaaat
     7201 cgattatgat tacattgtat ttgggttacc atactcatta tgatacttat gaggatatat
     7261 gttttatttt atctgtgtaa attataataa ctctgattat acagaagcat taattaagag
     7321 cgttattaat caagaaaaag atagctttgt aattgttgta gataactgtt ctaatgagca
     7381 agagctaaaa aagttaaatg atattgagag taaatataac gagaaggtca gattaattaa
     7441 aagtgatgtg aatttgggat attttggcgg attgaatctt ggcctaaaag gactaccaag
     7501 aaatcatcca atagttgttg ggaataatga tttggtttat gagagtaatt ttacgcaaat
     7561 aatatcacag actacatatc cggacgatgc tttagttatt gctccaaatg tgattacaaa
     7621 ggatggttat caccagaacc cacactgccg taagcgagta agtaagttta ggaaattttt
     7681 gtatgatata tatttttcaa attatatcgc tgctttagtt ttaacgtggg gaagcaggtt
     7741 tttaaattat ttgaaaggtg gaagaaataa ttcctttgat cagggtagag gttatataca
     7801 tatgggtatt ggagcctgtt atgtccttat gccttccttc ttcaaatatt ttgatgaatt
     7861 agataacaaa gtttttttat atggtgagga agcatatttg gctggccaat taatggaagt
     7921 aaatgggaag attttctatg agcctgatgc tattgtccat catgaagaaa gtgcaacttt
     7981 ggcgaaagtt gcttctaaaa caaaatatgg ctatatgaaa agctcatatt acgactataa
     8041 aaaatatctg taaaaggtga aaatatgttt aagaataaaa cactcgttat cactggtggc
     8101 actggttctt ttggtaatgc cgtacttaag cgttttctag atacagatat tactgaaata
     8161 cgaatattta gcagggatga aaaaaaacaa gatgatatgc ggaaaaaata taataactca
     8221 aaattaaaat tttatatagg tgatgtgcga gactataatt ccgttctaaa tgcaacgcgt
     8281 ggtgccgatt ttctgtatca tgcagcagcc cttaaacaag ttccttcatg tgaatttcac
     8341 cctatggagg cggttaagac aaatgttctg ggtacggaaa atgttctgga ggctgctatt
     8401 gcgaatggga ttaaacgcgt ggtgtgcttg agtaccgata aagccgttta tcctatcaat
     8461 gcaatgggca tatctaaggc aatgatggaa aaagttattg ttgcaaaatc acgtaatctt
     8521 gacagttcaa aaacagttat ctgtggaact cgttatggaa atgtaatggc ttcacgtgga
     8581 tcggtcatcc cattatttgt tgatctaatc aaagctggta aaccattgac cataaccgat
     8641 cccaatatga ctcgtttcat gatgacgctt gaggatgctg tcgatctggt cctttatgct
     8701 ttcgaacatg gaaataatgg tgacattttc gttcagaaag cacctgcggc aacaattcaa
     8761 acattagcca ttgcacttaa ggaattgcta aatgcccatg agcatccaat caatattatt
     8821 ggaactcgac acggggaaaa actttacgaa gcgttattga gccgagagga aatgattgca
     8881 gcggaagata tgggtgatta ttatcgtgtt ccaccagatc tccgcgattt gaactatgga
     8941 aaatatgtgg aacatggtga ccgtcgtatc tcggaagtgg aagattataa ttctcataat
     9001 actgagagat tagatgttga gggaatgaaa gaattactgc taaaacttcc ttttatccgg
     9061 gcacttcgtt ctggtgaaga ttatgagttg gattcataat atgaaaattt tagttactgg
     9121 cgctgcaggg tttatcggtc gaaatttggt tttccgcctt aaggaagctg gatataacga
     9181 acttattgcg atagatcgta actcttcttt gacggattta gagcagggac ttaagcaggc
     9241 agatttcatt tttcatcttg ctggagtaaa tcgtcctgtg aaggagagtg agtttgaaga
     9301 ggggaattgc gatctaactc aacagattgt tgatattctg aaaaaaaata ataaagatac
     9361 tcctatcatg cttagttcat ccatccaagc tgaatgtgat aacgcttatg gaaagagtaa
     9421 agcagttgcg gaaaaaatca ttcagcagta tggggaaacg acaaacgcta aatattatat
     9481 ttatcgcttg ccgaatgtat tcggtaagtg gtgtcaacca aattataact cctttatagc
     9541 aactttctgc catcgcattg caaatgatga agctattaca attaatgatc cttcagcagt
     9601 tgtaaatttg gtgtatatag atgacttttg ttctgacata ttaaagctat tagaaggagc
     9661 gaacgaaact ggttacagga catttggtcc aatttattct gttactgttg gtgaggtggc
     9721 acaattaatt tatcgattta aagaaagtcg ccaaacatta atcatcgaag atgtaggtaa
     9781 tggattcact cgagcattgt actcaacatg gttaagttac ctttctccta aacagtttgc
     9841 gtatacggtt ccttcttata gtgatgacag aggggtattc tgtgaagtat tgaaaacgaa
     9901 aaacgcgggc cagttttcgt tttttactgc gcatccagga attactcggg gggggcatta
     9961 tcatcattcc aaaaatgaga aatttattat catccgagga agtgcttgtt tcaaatttga
    10021 aaatattgtc acgggtgaac gatatgaact taatgtttca tcagatgatt ttaaaattgt
    10081 tgaaacagtt ccgggatgga cgcatgacat tactaataat ggctcggatg agctagttgt
    10141 tatgctttgg gcaaatgaaa tatttaatcg ttctgaacca gatactatag cgagagtttt
    10201 atcgtgaaaa aattgaaagt catgtcggtt gttgggactc gtccagaaat tattcgactc
    10261 tcgcgtgtcc ttgcaaaatt agatgaatat tgtgatcacc ttattgttca tactgggcaa
    10321 aattacgatt atgaattgaa tgaggttttt ttcaaggatt tgggtgttcg caaacctgat
    10381 tattttctta atgccgcagg taaaaatgca gcagagacta ttggacaagt tatcattaaa
    10441 gttgatgagg tctttgaaca ggaaaaacca gaagccatgt tagttcttgg cgatactaac
    10501 tcctgtattt cagcaatacc agcaaagcgt cgaagaattc cgattttcca tatggaggct
    10561 gggaatcgtt gttttgatca acgcgtaccg gaagaaacta acagaaaaat agttgaccac
    10621 accgctgata tcaatatgac atatagtgat atcgcgcgtg aatatctttt ggctgaaggt
    10681 gtaccagccg ataggattat taaaaccggt agcccaatgt ttgaagtact cactcattat
    10741 atgtcgcaga ttgatggttc cgatgtactt tctcgcctga atttaacacc tgggaatttc
    10801 tttgtggtaa gtgcccacag agaagaaaat gttgataccc ctaaacagct tgcgaaactg
    10861 gcaaatatac ttaataccgt ggctgaaaaa tatgatatcc cggtagttgt ttctactcat
    10921 ccgcgcactc gtaaccgcat caacgaaaac ggtattcaat ttcataaaaa tatcttgcta
    10981 cttaagccat taggatttca cgattacaac catctgcaaa aaaatgcacg tgctgtttta
    11041 tcggatagcg ggactattac agaggagtcc tccattatga acttccctgc gctcaatata
    11101 cgagaagcgc acgaacgccc tgaaggcttc gaagaagggg ctgtaatgat ggttggtctt
    11161 gagtctgccc gtgtgttaca ggcattagaa attattgcaa cacagcctcg tggagaagta
    11221 cgcttacttc gtcaggtcag tgactatagt atgccaaatg tttcagataa agttgtgcgt
    11281 attatccatt catatactga ctacgttaaa cgggttgtct ggaagcaata ctaatgaaac
    11341 ttgcattaat cattgatgat tatttgcccc atagcacacg agttggggct aaaatgtttc
    11401 atgagttagg cctggaattg ctgagcagag gccatgatgt aactgtaatt acgcctgaca
    11461 acacattaca ggcaatctat tctgttagca tgactgatgg tataaaggtt tggcgtttca
    11521 aaagtggacc tttaaaggat ataggtaagg ctaaacgtgc cataaatgaa actcttttat
    11581 cttttcgtgc atggcacgca ttaaagcatc tcatccaaca tgatacattt gatggtatcg
    11641 tttattattc cccctctatt ttttggggag gcttggttaa aaaaattaaa gagcgatgcc
    11701 agtgcccaag ctatctggtc ctaagggata tgttcccaca gtgggtcatt gatgctggta
    11761 tgataaaagc cggctcgcca attgaaaaat atttcaggta ttttgaaaaa aaatcatatc
    11821 aacaggctga ctggattggg ttaatgtctg ataagaatct tgagatattt cgtcaggcca
    11881 ataaaggtta tccgtgtgaa gttttacgta attgggcctc aatgactcct gtgtctgccg
    11941 gcgatgatta tcattcactt cgtcaaaaat acgctctaaa agataaaatc atttttttct
    12001 atggcggtaa tattgggcat gctcaggata tggcaaactt aatgcgcctt gcgcgtaata
    12061 tgatgcgtca tcatgatgct catttcctgt ttatagggca gggtgatgaa gttgacctga
    12121 ttaaatctct ttctgcagaa tggaatttaa ctaatttcac tcatctacct tcagtgaacc
    12181 aggaagagtt taaattaatt ttatctgaag ttgatgttgg cctgttctcc ctttcgtctc
    12241 gccattcttc acataatttc cccggaaaat tactagggta tatggttcaa tcaatcccga
    12301 tccttgggag tgtgaatggc ggtaatgatc taatggatgt aattaataag cacagggctg
    12361 ggttcattca tgttaatggt gaagatgata aactgtttga atctgcacaa ttgcttctta
    12421 gtgattcagt tttaagaaaa cagttaggtc agaacgccaa tgtgttgtta aagtctcaat
    12481 tttcggttga atcggcggca catactatcg aagtccgact ggaggcagga gaatgcgttt
    12541 agttgatgac aatattatgg atgaactttt tcgcactgca gcaaattctg aacgtttgcg
    12601 cgctcattat ttattgcacg catctcatca ggagaaggtt caacgtttac ttattgcatt
    12661 tgtacgcgac agctatgttg aaccccattg gcatgagtta ccgcatcagt gggaaatgtt
    12721 tgtcgtcatg caagggcaat tagaagtttg tttgtatgag caaaatggtg agctccaaaa
    12781 aaagtttgtt gttggagacg gtacgggaat aagtgtcgtg gaattttccc caggagatat
    12841 acatagtgtc aaatgcctgt caccaaaagc ccttatgtta gagataaagg aggggccatt
    12901 tgacccactg aaagctaagg ctttttctaa gtggttatag ggcgatacac caccgtttat
    12961 tcttctatct tattctatac atgctgggtt accatcttag cttcttcaag ccgcgtaacc
    13021 ccgcgtgacc acccctgaca ggagtaaaca atgtcaaagc aacagatcgg cgtagtcggt
    13081 atggcagtga tggggcgcaa ccttgcgctc aacatcgaaa gccgtggtta taccgtctct
    13141 attttcaacc gttcccgtga aaagacggaa gaagtgattg ccgaaaatcc aggcaagaaa
    13201 ctggttcctt actatacggt gaaagagttt gttgaatctc tggaaacgcc tcgtcgcatc
    13261 ctgttaatgg
//
