LOCUS       AB811608               14024 bp    DNA     linear   BCT 13-DEC-2014
DEFINITION  Escherichia coli DNA, O-antigen biosynthetic locus, strain: K1a.
ACCESSION   AB811608
VERSION     AB811608.1  GI:733166167
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. (2014) In press
   PUBMED   25428893
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14024)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..14024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="K1a"
                     /db_xref="taxon:562"
                     /note="serogroup:O28ab"
     gene            435..1826
                     /gene="wcaM"
     CDS             435..1826
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ00752.1"
                     /db_xref="GI:733166168"
                     /translation="MDKKFSRRRFLSAGAALAAVPLLHSPAARALGRNDAVDIRNYLV
                     GNDWIRTFRQAFDDADIIDVPKGVICDNINTGIIMPPGKTLQVNGTLKGNGRGRFALQ
                     DGCKVVGEGRLNNITLDVRGSDCTIEGLAMGGFGPVAQIYIGGKEKRTMRNLTITRIN
                     VSQANYGILRQGFHNQLDGVKITHSKFSHLQGDAIEWNVAINDKNILISDHAIDHIDC
                     TNGKTNWGIGIGLAGSTYDNDYPEHQAVKNFVVANITGSHCRQLVHVENGKHFIIRNI
                     KARNITPEFSKKAGIDNATVAIYGCDNFVIDGMDMVNSAGMLIGYGVIKGKYLSIPQN
                     FRLNNVRMDNRQLTLKLRGIQISSGNAPSFVALTNLDLNYASLEIHNKPQHIFMRNIK
                     VIQPSSRGPALQLHFDLRKDVRGKFMAKQETLLSMSNIHAVNEKGQQSVDIDRIDQHV
                     VNVDALNFTLPKR"
     gene            1961..2869
                     /gene="galF"
     CDS             1961..2869
                     /gene="galF"
                     /note="O028ab_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ00753.1"
                     /db_xref="GI:733166169"
                     /translation="MDTIMVNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEVVAAGIKEIVLVTHASKNAVENHFDTSYELEALLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQAQPLGLGHSILCARPIVGDNPFVVVLPDIILDGASADPLRYNLAAMVARYNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLTVEGKVSRIVEFIEKPDQPQTLDSDLMAVGR
                     YVLSADIWAELEKTAPGAWGRIQLTDAIAELAKKQSVDAMLMSGDSYDCGKKMGYMQA
                     FVQYGLRNLKEGAKFRKGIETLLNKD"
     CDS             3046..3873
                     /note="O028ab_02"
                     /gene="wfbZ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00754.1"
                     /db_xref="GI:733166170"
                     /translation="MTRIFMFSLGVIFTVYQKTDLSKFVKAIESVVNQTKLPQSIIIV
                     CDGCNSDLFKKAISGVVPHSIKLFFYSYSVNSGPGFARDFGIRKAEEDLIAVMDSDDV
                     SVADRFALQLAFFESNENISVCGGFIEEFDNLSGQRKLRRVPLQHSDIQNAIKIKSPV
                     NNVTAMFKRSDYLSSGGYPALRSSEDYSLWARFISSGYKIHNLDSVLVQVEFDIDAIQ
                     RRNGLLHFKNDFITQREMLKNGLIDKFEFVRNITKYCIFRFLPVGVKKMLYQHVLRK"
     CDS             3896..4288
                     /note="O028ab_03"
                     /gene="wfbX"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycerol-3-phosphate
                     cytidyltransferase"
                     /protein_id="BAQ00755.1"
                     /db_xref="GI:733166171"
                     /translation="MNRVITFGTFDVFHVGHINILERAASYGEELIVGVSSDALNFSK
                     KQRYPIYSESDRMKIISSLKFVTSVFLEESLELKLDYIKKYKASTLVMGDDWEGRFDW
                     VKSECDVIYLPRTPSISTTEIIEVVRKL"
     gene            4307..5566
                     /gene="wzx"
     CDS             4307..5566
                     /gene="wzx"
                     /note="O028ab_04"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ00756.1"
                     /db_xref="GI:733166172"
                     /translation="MIGLKKNIIFMLIYQIVNYLVPLALTPYLVRILGPSSYGELNYS
                     ISIVLYICLFVDFGFTLSATKRITKYIGNSDKISKLFYTVTVAKTFLLITGITILILS
                     ISTLDTINSVKTIIVISAIQIIATVISPLWLFNGLQLTAVFAVFNIIFKVASIPLVFF
                     LVKTPTDVGYAAFFQTAPLFFSSILALIYLYKKKILIYTNYTPRFKLIIRTLNSSLFY
                     YIGTMSVSLYTMSTPIILGLVSTSEQVGFYSASDKIRAAVVGVYVVIGNVIYPRVQRF
                     YHEDDNLGFQYVKKIIIAVIPLCTIASIMLFYISPFITKYFLGEEFGESELILKIMSP
                     MMFLIPLSIILSNYILLGMGHKKIFSRIPMITATIHIIVATILSLKFGSIGASIAILI
                     SEIVSFILLFITCCKLGYIKKLCVLKI"
     CDS             5548..6471
                     /note="O028ab_05"
                     /gene="wfeS"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00757.1"
                     /db_xref="GI:733166173"
                     /translation="MCTEDLISIIIPVFNAQETIDQCLNSIICCKYQNLEIIVINDGS
                     TDASSEIINDFSRRDERIKIITCENNGVSSARNIGIRHATGIWCCFIDSDDFISADYF
                     DILINDDACNYDIIQMKNIVFSPKQNNMNFFKRKQIFPMSGSAALKEKYAIYVWGKLY
                     KSSILKNTSFSRELSIGEDYYYNCQVFKNASIYYSNEGVYYYSLSPGSLSRGDSTGSK
                     IWERFKSANKIFYYLRDNDEKLALQIFTTFGFIGTLRAYSSFKWILYLIKEIDVEQIK
                     KIKFSAVWTSSCNLKAKILSTLVLCSMMVIK"
     gene            6468..7688
                     /gene="wzy"
     CDS             6468..7688
                     /gene="wzy"
                     /note="O028ab_06"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ00758.1"
                     /db_xref="GI:733166174"
                     /translation="MSIGILVLVFYVIVNFVMGIVFFNDGFLGGDFYGEKIDNKDLFL
                     WVLGGELLLLITFYAIAVLIVKFTFNKRYPEDYYTVSELALKRFNIFLIVLQTSFILF
                     IFATGFGRISLLMDENSSAANINSPIKYIFVILLPDFLFIVYFLVQKNNKKTIVNLSL
                     YLISNIVRGWVSGPVLLIMAIFLVKKFSFKKIKIKTIISLVTCFIIFVPLVTSLKFGV
                     RFQNDEISAVDLVQDSIENNVSYYLRTLNRFQHISEAYSFMDSMTFIRKDYADNRIAL
                     PFFTNDFINITSKIFSFEPQNLLTYGSKVLLDRPFGNIQSGILPWLSIDYFFSISYIL
                     TFFIFTILLSRFCKMLTLGYPVSKNIVIWFTIYYYAHGWLFSYMNLLIAMMIFIIFSK
                     VFGGKILNKDNNYA"
     CDS             7681..8859
                     /note="O028ab_07"
                     /gene="wfbV"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphotransferase"
                     /protein_id="BAQ00759.1"
                     /db_xref="GI:733166175"
                     /translation="MHKFQTLFWSVIFNILSLLFFLFPLKRNRVIFNSAENKNFNYNS
                     RYLFEFLKNYNNEYEIYFVINDADLRAKLSIEHGGKYFITTYSLKSCFLIMRSKFWII
                     STFESPYVCFVRHHRRIVFHLGHGIPLKAIGQLAKFPTKLKLKLQKILRIRPITHALC
                     YSEEYKTNMSAIFNSDFIEYVPLGQPRNDSIVLKDSVETGKLIKESILNVPSFDKAIL
                     YSPTWRPYDKARFFPFADLEANELNSILSQTKTMLFLRGHPFFESFCPDSFENLSNIH
                     WLNNDVIDDITSHLPFFDKIITDYSSIFIDYLCVNKPIGFLQYDYQQYDDMVGFATDD
                     REIFCGVKIESSDDFISFIIDQDDSWMPERLKIARLLNIKNSGNSLENKMFLDSLRKK
                     "
     gene            9008..10414
                     /gene="gnd"
     CDS             9008..10414
                     /gene="gnd"
                     /note="O028ab_08"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ00760.1"
                     /db_xref="GI:733166176"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVVA
                     ENPGKKLVPYYTVQEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNDELANT
                     FTEWNEGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPKAELAGDKAAFVEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENAGIANLLLAPY
                     FKNIADEYQQALRDVVAYAVQNGIPVPTFGAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWLD"
     gene            10647..11813
                     /gene="ugd"
     CDS             10647..11813
                     /gene="ugd"
                     /note="O028ab_09"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ00761.1"
                     /db_xref="GI:733166177"
                     /translation="MKVTISGTGYVGLSNGLLIAQNHDVVALDIIPSRVDMLNERISP
                     IVDKEIQQYLRSGELRFTATTDKYLAYQNADYVIIATPTDYDPKTNYFNTSSVESVIQ
                     DVIEINPQAVMIIKSTVPVGFTDSVKKKYATENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFARMLQQGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ETLGLNSRQIIEGVCLDPRIGDHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNIISAI
                     VDANRTRKDFIAEAILARKPKVVGIYRLIMKSGSDNFRASSVQGIMKRLKAKGITVIV
                     YEPVMKEDEFFSSRVVRDLDAFKQEADVIVSNRMAEELTDVAEKIYTRDLFGND"
     gene            11959..12957
                     /gene="wzz"
     CDS             11959..12957
                     /gene="wzz"
                     /note="O028ab_10"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ00762.1"
                     /db_xref="GI:733166178"
                     /translation="MDVTKVYMIQDNNNGRSGYEREPEQIDLIDLLIQLWRGKLTIIV
                     CVIVAIALAVAYVFLAKEKWTSTATITRPDAAQIATYHNAINTLDSSITLSDVQNQVI
                     NRFNSAFIALTEALDNQQEPEKLTIEPSVKGQNLPLTVTYVSDTAQSAQRGVAEYIQK
                     IDEQIADELELDLNDTIKLRVMNIKDDLENQETVAQEQKDLRIKQITESLKYAQKSNV
                     TKPQLQQTQDVTQETMFLLGTEALGAMVANEASRPLTFGAGYYGLKKNLLDIEKLKVD
                     PKTIHVYRYVMKPTLPIRRDSPKRGLTLVLAVLLGGMIGAGVVLGRNALRNYKPKA"
     gene            complement(13017..13616)
                     /gene="hisI"
     CDS             complement(13017..13616)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ00763.1"
                     /db_xref="GI:733166179"
                     /translation="MLTEQLDWDKTDGLMPAIVQHAVSGEVLMLGYMNKEALAQTESS
                     GKVTFYSRTKERLWTKGETSGNFLNVVSITPDCDNDTLLVLVNPIGPTCHKGTSSCFG
                     DASHQWLFLYQLEQLLAERKTADPESSYTARLYASGTKRIAQKVGEEGVETALAATVK
                     DRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     3927 a   2837 c   3091 g   4168 t
ORIGIN
        1 gcctgcgcac gctgatcgaa cagtatcaac tggaaggcta cgtggagatg ccgggtttca
       61 agcccagcca cgaggtaaaa gcgatgctcg acgacgcgga tgtgtttctg ctgccgtcaa
      121 ttaccggcgc ggatggcgat atggaaggca ttcccgtggc gctgatggaa gcgatggcgg
      181 tgggcatccc ggtgctctca accgagcata gcggtattcc ggaactgata gacgccaacg
      241 cctccggctg gctggtggcg gagaacgacg ccccggcact ggcgggcaaa ctcagcgaac
      301 tgcgccatct tgatgaacaa acgctgcgcc cggtgctcca acgcgcccgc gaaaaagtcg
      361 aaaccgagtt caatcagcag gtgattaacc gccagctggc gagcctgctg caaacccttt
      421 gctattgagg ttgcatggat aaaaaattct cccgacgtcg cttcctgtcg gcaggcgctg
      481 cgcttgccgc cgtacctctg ctccacagcc ccgctgcacg cgcgctgggg cgtaatgatg
      541 cagtcgatat ccgcaactat ctggtcggca atgactggat ccgtacattt cgtcaggcgt
      601 ttgacgacgc cgacattatc gacgtcccca aaggcgtgat ttgtgacaac atcaacaccg
      661 gcatcatcat gccgccgggt aaaacgctgc aggttaacgg caccctgaaa ggcaatggtc
      721 gtgggcgttt tgcgctgcag gatgggtgta aagtggtggg ggaggggagg ctcaataata
      781 ttaccctcga cgtgcgcggt tctgactgca ccatcgaagg gctggcgatg ggcggctttg
      841 gcccggtggc acaaatctat atcggtggca aagaaaaaag gacgatgcgc aatctcacca
      901 ttacgcggat taatgtcagc caggccaact acggtatttt acgtcaggga ttccataatc
      961 aactcgacgg cgtaaaaatc acccacagca aatttagcca tcttcagggc gatgccattg
     1021 aatggaacgt cgcgattaac gacaagaata tcctcatctc cgatcatgcg attgaccata
     1081 tcgattgcac caatggcaag accaactggg gtattggcat tgggcttgcg ggcagcacct
     1141 acgataacga ttacccggag catcaggcgg tgaaaaactt tgtcgtcgcc aacatcaccg
     1201 gtagccactg tcgacagctg gttcacgtcg aaaacggtaa acactttatt atccgaaata
     1261 ttaaggcacg aaatatcacc ccggaattta gtaagaaagc gggaattgat aacgcaacgg
     1321 tagcaatata tggatgtgat aatttcgtta tcgatggtat ggatatggtt aatagcgcgg
     1381 gtatgctaat tggctatggt gttatcaaag ggaaatatct gtcgataccg cagaatttca
     1441 ggttgaataa tgtgcggatg gataatcgcc aattaacgct aaagctgcgg ggaatacaga
     1501 tttcatcggg caatgcgccc tcgtttgtgg cgctcacgaa cctcgacctg aactatgcat
     1561 cgctggagat tcacaacaaa ccgcagcaca tctttatgcg aaatatcaaa gtgatacaac
     1621 cctcttcgcg cgggcccgcg ctacagctcc acttcgacct acgtaaggat gtacgcggga
     1681 agtttatggc caaacaggag acgctgctct cgatgtcaaa tattcacgct gtcaatgaaa
     1741 aaggccagca atcggtggat attgaccgta ttgatcagca cgttgtcaat gtcgatgcgc
     1801 tgaattttac cctacccaaa cgctgaattt ttagccgaac ccacctcttc agacgcattc
     1861 tcggattgta tccagagatt tagcggtatt tacccgatac tatgagaaaa catagtggat
     1921 caaacaacgt taatagctat aattcatcaa ccgtatatag gtggatacga taatggttaa
     1981 tttgaaagca gtcattccgg tagccgggct gggcatgcat atgctccctg ccaccaaggc
     2041 cattcctaaa gagatgctac cgatcgttga caagccgatg atccaataca tcgtcgacga
     2101 agttgttgct gcagggatca aagaaatcgt cctggtcacc cacgcttcta aaaatgccgt
     2161 ggaaaaccac ttcgacacct cctacgaact ggaagctctg ctggagcaac gcgtgaagcg
     2221 tcagctgctg gcggaagttc agtccatttg cccaccgggc gtaaccatca tgaacgtgcg
     2281 tcaggcacag ccgctgggcc tgggccactc cattctgtgc gcgcgtccga tcgttggcga
     2341 taacccgttt gtggttgttc tgcccgatat cattcttgat ggcgcatccg ccgacccgtt
     2401 gcgttataac cttgccgcga tggttgcacg ctataacgaa actggccgca gccaggtgct
     2461 ggcgaaacgt atgccgggcg atctttctga atactcggtt attcagacta aagagccgct
     2521 gaccgtagaa ggtaaggtga gccgtatcgt cgagtttatc gaaaaaccgg atcaaccgca
     2581 aacgctggat tccgacctga tggccgtagg gcgctatgtg ctttccgctg acatttgggc
     2641 cgaactggaa aaaaccgcgc cgggtgcctg gggacgtatt caactgacgg atgctatcgc
     2701 cgaactggcg aagaaacagt ccgttgatgc gatgctgatg agcggcgaca gctacgactg
     2761 tggtaagaaa atgggttaca tgcaggcgtt cgtccagtac gggctgcgca acctgaaaga
     2821 aggggcgaag ttccgcaaag ggattgagac gttgctgaac aaagattaat tttataatat
     2881 aagttattaa cttgtaggat tggaaaattt accaatccta cgattttagg tttttttttg
     2941 caaatttaag ttatgggctc tggtaacgat agctaggggc ggtagcgttt ctattcagtt
     3001 ttccttgaac aattttttga gtttagctta ttgtaacaaa taataatgac gagaatattt
     3061 atgtttagcc ttggcgtaat atttacagta tatcaaaaga cagatctttc gaaattcgtt
     3121 aaagctattg aatctgttgt caatcaaaca aagttgccac aaagtattat aatcgtttgt
     3181 gacggttgca actcagattt atttaaaaaa gctatatcag gggttgtccc tcatagcatc
     3241 aaattgttct tttactcata ttcagttaac tctggaccag gatttgcgcg tgattttggc
     3301 atcagaaagg cagaagaaga tttaattgct gttatggatt ctgacgatgt atcggtagct
     3361 gaccgatttg cattgcaact cgctttcttc gaaagtaatg aaaacatatc tgtttgtggt
     3421 ggttttattg aagagtttga taatctctcg ggtcaaagaa aattgagacg agtgccactg
     3481 caacattcag atattcaaaa tgctattaaa ataaagtcac ctgtaaataa tgttacagct
     3541 atgttcaagc gatctgatta tttatcttcg ggtggttacc cagcattacg aagttcagaa
     3601 gattattcat tatgggcccg ttttatttcc tcaggatata aaatacacaa tttagactct
     3661 gttttggttc aagtcgaatt tgatatcgat gcaattcaaa gaagaaatgg attattgcat
     3721 tttaaaaatg actttattac acaaagagaa atgcttaaaa atggattgat tgataagttt
     3781 gagtttgtaa gaaatattac taaatattgt atttttagat ttttaccagt tggtgtaaaa
     3841 aaaatgttat accagcatgt attaagaaaa tgaaatttaa atttggagtt gttgtatgaa
     3901 cagggtaatt acatttggaa ctttcgatgt gtttcatgtt gggcatataa atatcttaga
     3961 gagagcggct agttatggtg aggagctaat tgtaggggta tcttctgatg ccttaaattt
     4021 tagcaaaaaa caaagatatc cgatttactc tgagtccgat cgtatgaaaa tcattagttc
     4081 tttgaaattt gtcacctcag tttttcttga agaatcatta gagttgaaac ttgattacat
     4141 aaaaaaatat aaggcttcta ctcttgtaat gggggatgac tgggaaggtc gttttgactg
     4201 ggtgaaatca gaatgtgatg tgatatattt acctcgtacg ccttcaattt ctacaacaga
     4261 aataatcgaa gtcgtgagaa aattataact taaactagat ataataatga ttgggctaaa
     4321 gaaaaacatt atcttcatgt taatatacca gatagttaac tatctggttc cacttgcatt
     4381 gactccatat ttagttagaa ttttggggcc tagtagctat ggggaattaa attattcaat
     4441 atcaatagtt ttatatattt gtttatttgt tgattttggg tttacactta gtgcgactaa
     4501 gcgaattacg aaatatattg ggaactctga caaaatatcg aaattgtttt acactgtaac
     4561 tgtcgctaaa acctttttat taataactgg tattacgatc ctcatactta gtatttcgac
     4621 acttgacact ataaactcag taaaaactat aattgttata tcggctatcc agataatcgc
     4681 aacagtgatc tctccattat ggctatttaa tggactacag ctaacggccg tttttgcagt
     4741 ttttaatatt atatttaaag tcgcatctat accccttgtt ttttttcttg taaaaacacc
     4801 gacagatgtt ggttatgccg catttttcca aacggcacct ttatttttta gctctatttt
     4861 agctttgatt tatctgtata agaaaaaaat actgatttat acgaattata caccccgttt
     4921 taaattaatt atacgcacct taaattcatc acttttttat tatattggga ctatgtcagt
     4981 aagtctttat acgatgagca ctcctattat acttggatta gtgtcgacca gtgagcaggt
     5041 aggtttttat agtgcaagtg ataagattag ggctgcagtt gtcggtgttt atgttgttat
     5101 aggtaacgtc atttacccca gagttcagcg tttttatcat gaagatgata atttgggctt
     5161 tcaatatgtt aaaaaaatca ttatcgctgt aattcctttg tgcacaattg catcaataat
     5221 gttgttttat atttctccat ttattacgaa atattttctt ggtgaggaat ttggcgaatc
     5281 ggaattaata cttaaaataa tgtctccaat gatgttccta ataccactat ctatcatttt
     5341 gtcgaactat atattactgg ggatggggca taaaaaaata ttttcacgta taccgatgat
     5401 tacagcaact attcatataa ttgtagcaac gattctaagt cttaaattcg gttctattgg
     5461 agcatcaatt gcaatactta ttagcgaaat agttagtttt attttgcttt ttataacttg
     5521 ttgtaagtta ggatatatta aaaaattatg tgtactgaag atttaatatc aataattatt
     5581 ccagttttta atgcacaaga aacgatcgat caatgtctca atagtattat atgctgtaaa
     5641 tatcagaact tagaaataat tgttatcaat gatggaagca cagacgcgag tagcgagatt
     5701 ataaatgatt ttagcagaag agatgagcgg ataaaaatta taacttgcga aaacaatgga
     5761 gttagctcag ctagaaatat aggaattagg catgctacag gtatttggtg ttgtttcatt
     5821 gattccgatg attttatttc tgcagactat tttgacatac ttattaatga cgatgcttgt
     5881 aattatgata ttatccaaat gaaaaatatt gtattttctc caaaacaaaa taatatgaat
     5941 ttttttaaga gaaaacaaat ttttccgatg tctggctctg cagcattgaa agaaaaatat
     6001 gcgatctatg tctgggggaa attatataaa agttcaattt taaagaatac atctttctcg
     6061 agagaattat cgataggtga ggattattat tacaactgcc aagtctttaa aaatgcttct
     6121 atatattatt ctaatgaagg tgtttattac tattcgttaa gtcctggttc gctttcaaga
     6181 ggtgattcaa cagggagtaa aatttgggaa aggtttaaga gcgcgaataa aatattttat
     6241 tacttgagag acaatgatga aaaattagca ttacagattt ttaccacttt cggatttatt
     6301 ggaacattac gagcttattc ttcttttaag tggatcttgt atcttattaa ggagatagat
     6361 gtggaacaaa ttaaaaaaat aaaattttcc gcagtgtgga catctagttg taatcttaaa
     6421 gcaaaaatcc tatcgacatt ggtactatgt tcgatgatgg tgataaaatg agtattggta
     6481 tattggttct tgtattctat gttatcgtaa atttcgttat gggaatcgtc ttttttaatg
     6541 atggtttctt aggaggcgat ttctacggcg aaaaaatcga taataaagat ctgtttttat
     6601 gggtgttagg tggggaatta ttattattaa ttacatttta cgctattgca gtattaattg
     6661 ttaagttcac ttttaacaaa cgctatcccg aagattacta tactgtaagt gagttggctt
     6721 taaaaaggtt taatattttc ttaattgtac ttcaaacaag ttttattctg tttatttttg
     6781 cgactggttt tggaagaatt tcactgctta tggatgagaa ttcatctgca gcaaacatta
     6841 attcaccaat aaaatatatt tttgtaattc tactacctga ttttctattc attgtttatt
     6901 ttttagtaca aaaaaacaac aaaaagacaa ttgttaactt atccctttat ctgattagta
     6961 atattgtaag aggatgggtg tctggacctg tcctgctaat catggctatt tttttagtaa
     7021 agaaattttc atttaaaaaa ataaaaataa aaactattat ctctttagtt acatgtttta
     7081 taatttttgt acctttggtg acgtcattga aatttggtgt tagatttcaa aatgatgaaa
     7141 taagcgcagt ggatctcgtt caagattcta ttgaaaacaa tgttagctat tatcttagaa
     7201 ctctaaatag atttcagcat atatctgaag cgtatagttt tatggattcc atgacattta
     7261 ttcgtaaaga ctatgctgat aacagaatag cactcccatt ctttactaat gactttatca
     7321 atattacttc aaagattttt tcttttgagc ctcaaaatct attgacctat ggtagtaaag
     7381 ttctgcttga tagaccattc ggtaatatac aatctggcat tcttccttgg ctctctattg
     7441 attatttctt ttcaatctct tatattttaa cattttttat atttacaatt ttgctttccc
     7501 gtttttgtaa aatgctaact cttggctatc cggtttcaaa aaatatagtt atttggttca
     7561 ctatatatta ctatgcgcat gggtggttgt tttcgtatat gaacttacta atagcaatga
     7621 tgatttttat tattttttcc aaggtctttg gaggtaagat tttaaataag gataataatt
     7681 atgcataaat tccaaacatt gttttggtct gttattttta acatactatc tcttcttttt
     7741 tttctttttc cattgaaaag aaatagagtt atttttaatt cagctgagaa taagaatttt
     7801 aattataact ctagatacct ttttgagttt ctaaaaaact ataataatga gtacgaaata
     7861 tacttcgtca ttaatgatgc tgacttaagg gcgaaattat caattgagca tggcggaaaa
     7921 tacttcataa ccacgtattc attaaaatca tgttttctaa ttatgcgttc gaagttctgg
     7981 atcatttcaa catttgaaag tccctatgtt tgttttgtcc gccatcatag acgtatagta
     8041 tttcatttgg ggcatgggat tccacttaag gctattggac aattagctaa gtttccaaca
     8101 aaattgaaac ttaagctgca aaaaatttta agaattcgcc ccattacaca cgctttatgc
     8161 tactctgaag aatacaaaac aaatatgagt gccattttta attcggactt catagaatat
     8221 gttcctttgg ggcagccacg taatgactcg attgttctca aagattcagt agaaactggc
     8281 aagttaataa aagaatccat tctcaatgta cctagttttg ataaagccat attgtactct
     8341 cctacatggc gtccatatga taaggcaagg tttttcccat ttgccgattt agaagcgaat
     8401 gaattgaata gtattctaag tcaaacaaag acaatgctat ttttaagagg gcatcctttt
     8461 tttgaatctt tttgcccaga tagctttgaa aatttatcca atatacattg gcttaataat
     8521 gatgtcattg atgacattac ttcacatttg cctttttttg ataagattat cactgattat
     8581 tcaagtatat ttattgatta tttatgtgtt aataaaccaa ttggtttttt gcaatacgat
     8641 tatcaacagt atgatgatat ggtcgggttc gcaactgatg atagagagat tttttgtggc
     8701 gtaaaaattg aatcttccga tgatttcatt tccttcataa ttgaccaaga tgattcttgg
     8761 atgccagagc gtttaaaaat cgcacggtta ctgaatataa aaaatagtgg taatagctta
     8821 gaaaataaga tgttcctaga tagtttacgg aaaaaataaa agagacttat taggtttttt
     8881 cctttcgtgc caattaaata aattatctaa aacaaaagct attcacaata atatgtttta
     8941 agatagaatt cttagtgttt tctaatctgc gcaaatgcgc ggtgaccaca cctgacagga
     9001 gtatgtaatg tctaagcaac agatcggcgt agtcggtatg gcagtgatgg ggcgcaacct
     9061 ggcgctcaac atcgaaagcc gtggttatac cgtctccatc tttaaccgct cccgtgaaaa
     9121 aaccgaagaa gttgttgccg aaaatccagg caaaaaactg gttccttatt acacggtaca
     9181 ggaattcgtc gaatctcttg aaacccctcg tcgtatcctg ttgatggtga aagcaggcgc
     9241 gggcaccgat gccgcaattg attccctgaa accgtatctc gataaaggtg acatcatcat
     9301 tgatggcggt aacaccttct tccaggacac cattcgtcgt aaccgtgaac tctccgctga
     9361 aggtttcaac ttcattggga ccggtgtttc cggtggtgaa gagggcgcgc tgaagggccc
     9421 atccatcatg cctggtggtc agaaagaagc ctatgagctg gttgccccaa tcctgactaa
     9481 aattgctgca gttgcggaag atggtgagcc gtgcgtaacc tatatcggcg ctgatggtgc
     9541 gggtcactat gtgaaaatgg tgcacaacgg catcgaatac ggtgatatgc agctgatcgc
     9601 agaagcctat tctctgttga aaggtggtct gaacctctct aacgacgagc tcgcgaacac
     9661 cttcaccgag tggaatgaag gtgagctgag cagctacctg attgacatca ccaaagatat
     9721 cttcaccaag aaagatgaag acggtaacta tctggttgat gtgattcttg atgaagctgc
     9781 taacaaaggt accggtaagt ggaccagcca gagctctctg gatctcggcg agccgctgtc
     9841 cctgatcacc gagtccgtat tcgcgcgtta tatctcttct ttgaaagatc agcgtgttgc
     9901 agcctctaaa gtgctttccg gcccgaaagc ggaactggct ggtgacaaag cggctttcgt
     9961 tgagaaagtt cgccgtgcac tgtatcttgg taaaatcgtt tcttacgcgc agggcttctc
    10021 tcagctgcgt gccgcgtctg acgaatacaa ctgggatctg aactacggtg aaatcgcgaa
    10081 gatcttccgt gctggctgca tcattcgcgc gcagttcctg cagaagatca ccgatgccta
    10141 cgctgaaaat gccggtattg ctaacctgct gctggcgccg tacttcaaaa atatcgctga
    10201 tgagtatcag caggcgctgc gtgacgtggt ggcctacgct gtacagaacg gtatcccggt
    10261 tcctaccttc ggcgctgcgg ttgcctacta tgacagctac cgttcagcgg tactgccggc
    10321 taacctgatt caggctcagc gtgactactt cggtgcgcat acctataaac gtactgataa
    10381 agaaggcgta ttccataccg aatggttgga ctaatcctaa attaatatag aaagcccggc
    10441 gatgccgggc ttttttttgt gctaaattct tattgcgtta tcgttctgtc ttaatattaa
    10501 cttaatatcg acggtttatt ctctctgaag aaagtccgaa ttgtttgtaa ttctattgca
    10561 gtgaatgcgc ttacgagtta aaaaatttta aacgtattga tgtgatatca cattataaat
    10621 aattaagttt attctgagag agtaaaatga aagttaccat ctctggtaca ggctaygtcg
    10681 gtctctcgaa cggcctgctg attgcgcaaa accatgacgt cgtggcgctg gatatcattc
    10741 cttcacgtgt agatatgctc aatgagcgta tctcgcccat tgtggacaag gagatccagc
    10801 agtatctgcg ttcaggggag ctgcgtttca ccgcaaccac tgataaatac cttgcttatc
    10861 agaatgctga ttacgttatt atcgcgacac caacggatta tgaccctaaa actaactatt
    10921 ttaatacgtc cagcgttgag tcggttattc aggatgtcat tgagattaat ccgcaagccg
    10981 tgatgattat taaatcaacg gtgccggttg gttttacgga ttcggtgaag aaaaaatatg
    11041 ccactgagaa tattattttc tcaccagagt ttttgcgcga aggtaaagcg ttgtacgaca
    11101 accttcaccc atcgcggatc gtgattggtg agcgttcaga gcgcgcagaa cgttttgcca
    11161 ggatgctcca gcagggggcg attaagcagg acatccccac attgtttacc gattcgacgg
    11221 aagcagaggc gattaaactc tttgccaaca cttacctggc aatgcgcgtc gcttacttta
    11281 acgagctgga tagctatgcg gaaactctgg gtctcaattc ccgccaaatc atcgaaggcg
    11341 tttgcctcga cccgcgcatc ggcgatcact acaacaatcc ctcctttgga tatggcggct
    11401 actgcctgcc gaaggatacc aagcagctac tagcgaacta ccagtcggtg cctaacaaca
    11461 tcatctccgc cattgtcgat gctaatcgta cgcgcaaaga ctttatcgct gaggcgattc
    11521 tggcgcgtaa accgaaagtt gtcggtatct atcgcctgat tatgaaaagc ggctccgata
    11581 atttccgtgc atcctccgtg cagggcatca tgaaacgcct gaaagcgaag gggataacgg
    11641 tgattgtgta cgaaccggtg atgaaagagg atgaattctt cagctcacgt gtcgttcgcg
    11701 atctggatgc cttcaagcaa gaagcggatg ttatcgtttc taaccgtatg gccgaggagt
    11761 taaccgacgt agcagaaaaa atctacacgc gcgatctttt tggcaacgac taatctaaat
    11821 ttttgacaag ctgaatgcct gacaagacgg gcgttcagcc ttttttctga ttcttttcag
    11881 gtttctctgt ttttggtgaa aatcctatag aaattcggat tggtccaggt aaactgatgc
    11941 cctcgtgggt atttcagttt ggatgtcact aaggtttata tgattcaaga taacaataat
    12001 gggcgcagcg ggtatgagcg tgagccggag cagatcgacc ttatcgattt gttgatacaa
    12061 ctctggcgtg gcaagttgac catcatcgta tgcgtcatcg tcgcgattgc gctcgctgtg
    12121 gcttatgtct ttttagcgaa agagaaatgg acctctaccg cgaccatcac tcgcccggat
    12181 gcggcgcaga ttgcgaccta ccataacgcc ataaatactc tggattcatc gattaccttg
    12241 tccgacgttc aaaaccaggt gattaaccgc tttaactctg catttatcgc attgacggaa
    12301 gcgttggata atcagcaaga gcctgagaaa ctgactattg agccctctgt taagggccag
    12361 aatctacccc taactgtgac ttacgtgagt gatacagcac aatccgccca gcgcggtgta
    12421 gctgaataca tccagaaaat cgacgaacaa atcgctgatg agctggaact tgaccttaat
    12481 gacaccatca aattacgtgt gatgaacatt aaggacgacc tggaaaacca ggaaaccgta
    12541 gctcaggaac aaaaagacct gcgtatcaag cagatcactg aatccctgaa atacgcgcag
    12601 aaaagtaacg tcaccaaacc gcagcttcag cagacccaag acgtaactca ggaaaccatg
    12661 ttcctgttgg gcacagaggc tctgggcgca atggttgcca acgaagccag ccgtccgctg
    12721 acctttggtg cgggctacta cggtttgaag aaaaacctgc ttgatatcga aaaactgaaa
    12781 gtcgatccga aaaccattca cgtctatcgc tacgtgatga agccgacgct gccaatccgt
    12841 cgcgacagcc cgaagcgtgg tctgacgctg gtgctggcgg tgctgctggg cgggatgatt
    12901 ggtgccggtg ttgtgttggg ccgcaacgcg ttgcgtaact acaaaccgaa agcataacgc
    12961 ttacgtcaga tagcaaaaaa ccgggctcgc ccggtttttt tacgcctgtt gaaaatttac
    13021 tgatgacgtt ttcgcaggtt ctcaatcacg gtcgttaaat cgagatcctg atcctgcagc
    13081 agcaccagca ggtggtacat caagtccgac gcttcattgg tcagctcaaa gcggtctttc
    13141 accgttgctg ccagtgcggt ttctacgcct tcttcaccca ctttctgcgc gatacgcttg
    13201 gtgccgctgg catacaggcg cgcggtgtag gagctttccg ggtctgcggt tttacgctcg
    13261 gccagcagtt gttccagttg atacaggaac aaccattggt ggctggcgtc gccgaagcaa
    13321 ctggaagtgc ctttgtggca agttggccca atcgggttga ccagtaccag cagggtgtcg
    13381 ttgtcgcagt cgggcgtgat gctgaccacg ttcaggaaat tgccggaggt ttcacctttg
    13441 gtccacaggc gttctttggt gcgcgaataa aacgtcactt tgccggacga ctctgtctgg
    13501 gccagcgcct ctttattcat atagcccagc attaatactt cgccggaaac cgcgtgttgt
    13561 acgatggcgg gcatcagtcc gtcggtttta tcccaatcca gttgttcagt taacataccc
    13621 gtatctccac gccctgtgcg gccaggtacg cttttaattc accaatatta ataatttgct
    13681 tgtggaatac cgaggcggcc agcgcgccat cgacatccgc atcgcggaag gcttcgagga
    13741 agtgttccat ggtacccgca ccgcccgagg caatcagcgg tacgtggcag acgtcacgca
    13801 cttttttcag ttgcgccagg tcgtagccat tacgcacgcc gtcctggttc atcatgttga
    13861 ggacgatctc gcccgcgccg cgtttctgta cttcctgcac ccagtcgagg gtttcccatt
    13921 gggtgacgcg ggtgcggctt tcatcgccgg tgtattgatt gacgtgatat ttgccgctgt
    13981 cggcgtcaaa ccaggtgtcg atcccgacca cgatgcactg cacg
//
