LOCUS       AB811609               15777 bp    DNA     linear   BCT 13-DEC-2014
DEFINITION  Escherichia coli DNA, O-antigen biosynthetic locus, strain:
            Kattwijk.
ACCESSION   AB811609
VERSION     AB811609.1  GI:733166180
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. (2014) In press
   PUBMED   25428893
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15777)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..15777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Kattwijk"
                     /db_xref="taxon:562"
                     /note="serogroup:O28ac"
     gene            237..1628
                     /gene="wcaM"
                     /pseudogene="unknown"
     CDS             237..1628
                     /gene="wcaM"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     gene            1763..2668
                     /gene="galF"
     CDS             1763..2668
                     /gene="galF"
                     /note="O028ac_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ00764.1"
                     /db_xref="GI:733166181"
                     /translation="MDTIMVNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEVVAAGIKEIVLVTHASKNAVENHFDTSYELEALLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQAQPLGLGHSILCARPIVGDNPFVVVLPDIILDGASADPLRYNLAAMVARYNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLTVEGKVSRIVEFIEKPDQPQTLDSDLMAVGR
                     YVLSADIWAELEKTAPGAWGRIQLTDAIAELAKKQSVDAMLMSGDSYDCGKKMGYMQA
                     FVQYGLRNLKEGAKFRKGIEKLLSE"
     CDS             2996..3391
                     /note="O028ac_02"
                     /gene="wfxA"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00765.1"
                     /db_xref="GI:733166182"
                     /translation="MKKIITFGTFDVFHVGHVNILERAASLGDYLIVGVSSDELNYSK
                     KQRYPIYSQEDRTKIISSLKFVNEVFIEESLEKKLEYIKTYNADILVMGDDWAGRFDW
                     VKSDCEVIYLPRTPSISTTEIIEVVKSIR"
     gene            3402..4631
                     /gene="wzx"
     CDS             3402..4631
                     /gene="wzx"
                     /note="O028ac_03"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ00766.1"
                     /db_xref="GI:733166183"
                     /translation="MKLKQNVLFGNIAALAILQLATYITPLLIVPYLTRVLGPGGYGT
                     VAICLSIIQMSLILTDFGFNLSSTEYIARNCQSKDKINKHIGAVFQIKILLIFLCVIF
                     LYFLVNDQLQQFKIEFFFYSCIAIIGQTFQSLWFFQGVEKMKRITLFLTISKFSYVIL
                     TFVIVKKPEQGRFVILAYALSQVIGALLSQYYMRKEGYKYTPANLSSMLGVLRTSALF
                     FWSRLAVSTYTTASTLVVGAAGHVQAGYYAASEQLYKAGQNITTPVSQALYPYMTRAK
                     NWALFFKMMSILTVVMITASLTAIWLSQWLIALIFGPEYIVSQNVFIVFMLALIVTFV
                     STNFGYPVFSALGKVKVANYTVIFGALTHVVILGVLFCLGEISAYNVVRAVLFTEIVI
                     LILRMFFFVKLYRREVK"
     CDS             4628..5806
                     /note="O028ac_04"
                     /gene="wfxB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycerophosphotransferase"
                     /protein_id="BAQ00767.1"
                     /db_xref="GI:733166184"
                     /translation="MIIKKVISKLFGLFFCALDYVWKKQDINIFGAGRGSRYFDNSKT
                     LFEYYISQGDRAYWFYTGKKPDVVGISPDAFVQANSLYGMLIALRAKKAFACYGSLDF
                     WLYRFSRKTVSVQLWHGIPIKKVFFGEKNLTQRKKLQYLFEISAFQYFIVSSKLEQYI
                     ISAQTGMHLDRVKVLGYPRNDNLFKSANEVIIPEVVALNSNFKKVVLFAPTFRNNDYS
                     VFSSFNDDEWNVFFDFLKTENFLCIFRAHAVEFLQNNDDNAKRLSKYSSNVIFLGQDK
                     YQDAQDLMVYSDILISDYSGMIPDYLLLDKPIIRFIFDEQQYDNERGVNFLQKNLPIG
                     SAAYNMPTLIEALRQCVESTEESVAVNAFAKKMYHDNLCGKYSEKIYNYFNSLSQGKV
                     "
     gene            5803..7182
                     /gene="wzy"
     CDS             5803..7182
                     /gene="wzy"
                     /note="O028ac_05"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ00768.1"
                     /db_xref="GI:733166185"
                     /translation="MKQARIKPFSYLSLSFFVLFLIFLNISAVSFDEYLTDLQYSYAY
                     VLFAISLVAVYYAQGESYTSLGFLFNTCLFLFIGGRFIATTFGIDSESVFTLDFMTTY
                     IPTSDEKLEIYTRVVSFQIFLNLGYLFLRKEDSLLNLNKNDNSLPGRLLFLIVIVLFL
                     LCLVEQYSMLSKAMSEGYLARYGDQAESYDAGAAFKYTLLFVFTGLLFATCKKKYIII
                     NFMIYMTLATIIILIGARGAFVSTILMGVWLYGLNKKISLKKLSIIFLSLAGILLIIM
                     NFSARGNYYSNELIDSLLRFLYDQGNTLAVVGYVRTITDYPVFAYISTFLPGSSFFAS
                     MFHGGSDLNSYMLSFPAYANYVANPNMYFAGYGLGWSIISSVEMFSADILFIFCIFSV
                     LIGMFLKFANEQAKRNSFWFGAVACLCAKILFTARADIKTIIPLFMYYAIMYLIISTA
                     MRILRQRYG"
     CDS             7175..8185
                     /note="O028ac_06"
                     /gene="wbeX"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00769.1"
                     /db_xref="GI:733166186"
                     /translation="MDNRDVMFSVIVPCYNVVSHINETVASLNKAIDSACTTVEVIFV
                     NDGSTDKTLDVLNEICSNKPSYRVIDQENGGVSNARNTGIRESTGKYILFLDSDDTYK
                     NNIFSILSNISFSDDIVFFSYEKIDQSGNTRVYTIPNSYMAENSITVLKDLFSKKIYL
                     NICALAVNRDCIFQNNIFFDESIQHCEDLQFIIKMITYSKSFQFINEPLFTYNYTPGS
                     AVNSKVDEKHYSKFIAFENMRDVFIKNDKYKELIPAYDFYVATVYLLLLKTIVSNGVV
                     NNKQLSKFVEYSFILKRKMELPPNTMGAFVLIFTIINYIFPMKVKFLMMSFILRMNKG
                     LR"
     CDS             8182..9099
                     /note="O028ac_07"
                     /gene="wfdK"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00770.1"
                     /db_xref="GI:733166187"
                     /translation="MSITNKTIALVIVTYNRCNLLMEMLSSIENMSVKPDIVYVIDNN
                     SSDNTSSVVTECDSRKNINIKYHNTGYNAGGAGGFYIGSKMAYEDGWDRIWLADDDIV
                     LDKECLSNAMEYDDGRTILQPMRYNMDGSCAEISAIQYDLSNPFYLRPKRKTVQNIFN
                     KNILSYDIQSIPFEGPIIPREVFNVIGFPDERFFIFNDDLDFAIRAQRAGFSIKCITN
                     AKIVRKIPFVQSVALKTWKGYFMFRNYFRVQKVYGSSPLIYLRILLVFCLALGHSLVR
                     MDINSIKMLCGALKDGLSQEFKLTEKYKP"
     CDS             9096..10166
                     /note="O028ac_08"
                     /gene="wfxC"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="BAQ00771.1"
                     /db_xref="GI:733166188"
                     /translation="MSTTDSPNIKTSNNNFDIVRLILALVVVFSHIRDLNNINSRFFS
                     IFSGDFAVECFFVISGYLIIRSFKNNQNIRKYIFSRGMRILPMYYVSIVSFFVILFFI
                     SHLSVVEYLKEGGIRYLVFNGVFLNFIQPSLPGVFVDNPRISAVNGALWTIKVEILFY
                     IFVPFFVLFIKKINRHIIPIFIILILSSIVFKWIVTDYHQILHIPEQLVNQLPSVLSY
                     FLLGGLVNYLDLSKISKKTVFWFFIVSAFYLLLSSKTSLDLLVRPFVIAGFILPICLQ
                     RKFLISVPEKMGDLSYGIYVTHFPLVQLLIAVGLYNSFWLGFCLTFTSLFILSFISWH
                     LIEKPALRFAKSAGKNKVVRGV"
     gene            10163..11308
                     /gene="gif"
     CDS             10163..11308
                     /gene="gif"
                     /note="O028ac_09"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="BAQ00772.1"
                     /db_xref="GI:733166189"
                     /translation="MRIAIVGAGFSGAVIARELAEHGHSVDIFEKRDHVAGNCYSKRD
                     PDTNVMVHVYGPHIFHTDDKEVWEYINKFGMFEPYVNRVKAITKGAVYSLPINLHTIN
                     QFFHKTLNPAEAKDFINSLCELFEFEPITFEQQALSFIGRDLYEAFFRTYTIKQWGLE
                     PSELPASILKRLPVRFNYNDNYFSHRYQGMPRDGYTNIISAILDHASIAVKLNTDFKK
                     NVGNEYDHVFYSGTIDGYFDYSHGRLPYRTLDFVTERHKGDYQGTAVINYCDDSEKFT
                     RITEHKYFSPWEEHSNTIIYKEYSRTCTETDSPYYPIRQVQEKELLRKYVDLANQTNN
                     VSFIGRLGTYRYLDMDVTIREALNAVKLFMKIKKENEKVPAFFTEIL"
     gene            11487..12893
                     /gene="gnd"
     CDS             11487..12893
                     /gene="gnd"
                     /note="O028ac_10"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ00773.1"
                     /db_xref="GI:733166190"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVVA
                     ENPGKKLVPHYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNDELATI
                     FTEWNEGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPKAELAGDKAAFVEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENSGIANLLLAPY
                     FKNIADEYQQALRDVVAYAVQNGIPVPTFGAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWLD"
     gene            13127..14293
                     /gene="ugd"
     CDS             13127..14293
                     /gene="ugd"
                     /note="O028ac_11"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ00774.1"
                     /db_xref="GI:733166191"
                     /translation="MKVTISGTGYVGLSNGLLIAQHHDVVALDIIPSRVDMLNARVSP
                     IVDKEIQQYLSSGELRFSATTDKYLAYQNADYVIIATPTDYDPKTNYFNTSSVESVIQ
                     DVIDINPQAVMIIKSTVPVGFTDSVKKKYATDNIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFARMLQQGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ETLGLNSRQIIEGVCLDPRIGAHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNIISAI
                     VDANRTRKDFIAEAILARKPKVVGIYRLIMKSGSDNFRASSVQGIMKRLKAKGITVIV
                     YEPVMKEDEFFSSRVVRDLDAFKQEADVIVSNRMAEELTDVAEKIYTRDLFGND"
     gene            14439..15437
                     /gene="wzz"
     CDS             14439..15437
                     /gene="wzz"
                     /note="O028ac_12"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ00775.1"
                     /db_xref="GI:733166192"
                     /translation="MDVTKVYMIQDNNNGRSGYEREPEQIDLIDLLMQLWRGKLTIIV
                     CVIVAIALAVAYVFLAKEKWTSTATITRPDAAQIATYHNAINTLDSSITLSDVQNQVI
                     NRFNSAFIALTEALDNQQEPEKLTIEPSVKGQNLPLTVTYVSDTAQSAQRGVAEYIQK
                     IDEQIADELELDLNDTIKLRVMNIKDDLENQETVAQEQKDLRIKQITESLKYAQKSNV
                     TKPQLQQTQDVTQETMFLLGTEALGAMVANEASRPLTFGAGYYGLKKNLLDIEKLKVD
                     PKTIHVYRYVMKPTLPIRRDSPKRGLTLVLAVLLGGMIGAGVVLGRNALRNYKPKA"
     gene            complement(15497..>15777)
                     /gene="hisI"
     CDS             complement(15497..>15777)
                     /gene="hisI"
                     /codon_start=3
                     /transl_table=11
                     /protein_id="BAQ00776.1"
                     /db_xref="GI:733166193"
                     /translation="WLFLYQLEQLLAERKTADPESSYTARLYASGTKRIAQKVGEEGV
                     ETALAATVKDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     4442 a   3084 c   3484 g   4767 t
ORIGIN
        1 cgaccgagca tagcggtatt ccggaactga tagacgccaa cgcctccggc tggctggtgg
       61 cggagaacga cgctccggcg ctggcgggca aactcagcga actgcgccat cttgatgagc
      121 cgacgctgcg cccggtgcta caacgcgccc gccaaaaagt tgaaaccgag ttcaatcagc
      181 aggtgattaa ccgccagctc gcgagcctgc tgcaaaccct ttgctattga ggttgcatgg
      241 ataaaaaatt ttcccgacgt cgcttcctgt cggcaggcgc tacgcttgcc gccatcccac
      301 tgctacacag ccccgctgta cgcgcgctgg ggcgtaatga tgccgttgat atccgcaact
      361 atctggtcgg taatgactgg atccgtgcat ttcgtcaggc gtttgacgat gccgatatta
      421 tcgacgtccc caaaggcgtg atttgcgaca atattaatac cggcatcatc atgccaccgg
      481 gtaaaacgct gcaggtaaac ggcagcctga aaggcaatgg tcgtgggcgt tttgcgttgc
      541 aggatgggtg taaagtggtg ggggagggga gtctcaataa tattactctc gacgtgcgcg
      601 gatctgactg caccatcgaa ggtctggcga tgagcggctt tggcccggtg gcgcaaatct
      661 acatcggcgg caaacaaaaa aggacgatgc gcaatctcac catcacgcgg attaatatca
      721 gccaggctaa ctacggtatt ttacgccagg gtttccataa ttaactcgac ggcgtaaaaa
      781 tcacccacag taaatttagc tacctgcagg gtgatgccat tgaatggaac gtcgcgatta
      841 acgacaagaa tatcctcatc tccgatcatg tgattgacca tatcgattgc accaatggca
      901 agaccaactg gggtattggc attgggcttg cgggcagcac ctacgataac aattacccgg
      961 agcaccaggc ggtgaaaaac tttgtcgtcg ccaatatcac cggcagccac tgccgacagt
     1021 tggttcacgt cgaaaacggt aaacacttta ttatccgtaa tattaaggca cgaaatatca
     1081 ccccggaata cagtaagaaa gccggaattg ataacgctac ggtagcaata tatggatgtg
     1141 ataatttcgt tatcgatggt gtggatatgg ttaatagcgc gggcatgcta attggctatg
     1201 gtgttatcaa agggaaatat ctgtcgatac cgcagaattt caggataaat aatgtgcgga
     1261 tggataatcg acaattaacg ctaaagctgc ggggaataca gatttcatcg ggcaatgcgc
     1321 cctcgtttgt tgcgcttacc aacctcgatc tgaaccatgc gtcgctggag atccacaaca
     1381 agccgcaata catatttatg cgtaatatca aggtgataca gcctgcggcg cgcgggcccg
     1441 cactacagct ccacttcgac ttacgcaagg atgttcgcgg gaagtttatg gccaaacagg
     1501 agacgctgct ctcgatgtca aatattcatg cggtcaatga aaacggccag cggtctgtgg
     1561 atattgaccg tattgatcag cacgttgtca atgtcgatgc gctgaatttt accctgccta
     1621 aacgctgaat ttttagccga acccacctct tcagacgcat tctcggattg tatccagaga
     1681 tttagcggta tttacccgat actatgagaa aacatagtgg atcaaacaac gttaatagct
     1741 ataattcatc aaccgtatat aggtggacac gataatggtt aatttgaaag cagtcattcc
     1801 ggtagccggg ctgggcatgc atatgctccc tgccaccaag gccattccta aagagatgct
     1861 accgatcgtt gacaagccga tgatccaata catcgtcgac gaagttgttg ctgcagggat
     1921 caaagaaatc gtcctggtca cccacgcctc caaaaatgcc gtggaaaacc acttcgacac
     1981 ctcctatgaa ctggaagctc tgctggagca acgcgttaag cgtcagctgc tggcggaagt
     2041 tcagtccatt tgcccgccgg gcgtaaccat catgaacgtg cgacaggcgc agccgctggg
     2101 cctaggccac tccattttgt gcgcgcgtcc gatcgttggc gataacccgt ttgtggttgt
     2161 tctgcctgat atcattctcg atggcgcatc cgctgaccca ttgcgttata acctcgctgc
     2221 gatggtggca cgttataacg aaacaggccg cagccaggtg ctggcgaaac gtatgccggg
     2281 cgatctttct gagtactcgg ttattcagac taaagagccg ctgaccgtag aaggtaaagt
     2341 gagccgtatc gttgagttca tcgaaaaacc agaccaaccg caaacgctgg attccgacct
     2401 gatggccgta gggcgctatg tgctttccgc cgacatctgg gccgaactgg aaaaaaccgc
     2461 gccgggtgcc tggggacgta ttcagttgac ggatgctatc gccgagctgg cgaagaaaca
     2521 gtccgttgac gcgatgctga tgagcggcga cagctatgac tgcggtaaga aaatgggtta
     2581 catgcaggcg tttgtccagt atgggctgcg caacctgaaa gaaggggcga agttccggaa
     2641 agggattgag aaactgcttt cagaataaga cactacgcaa caacacggaa tgttggtgat
     2701 gtaacagaac ggcagatgat tgtggttgag gaactctcat ttcacagcag ttgccgtttt
     2761 tgcttttaaa aacaacaact aaaattagtg attgcgcggt gttttttgat aaaaaatgcc
     2821 ggatttctcc ttgtcttaca cagggattgc gcccaaaata gtgggctaaa ttggtgggcg
     2881 agattcgatg caaacgtgaa ggtttgtggg ggctggtcca cgacatactg aattttgtgc
     2941 agtgcactgg tagctgttga gccaggggcg gtagcgtgaa tattatgaga gagctatgaa
     3001 gaaaattata acctttggga cttttgacgt tttccatgtt gggcatgtga atattcttga
     3061 aagggctgcc agtcttggcg actatctaat agttggcgtc tcttctgatg agttgaatta
     3121 ttcaaaaaaa caacgctatc ctatttattc tcaagaagat cgaaccaaaa ttatttcatc
     3181 actaaagttt gtcaatgaag ttttcataga agaatccctt gagaaaaagc tcgagtacat
     3241 taaaacatat aacgcggata ttcttgttat gggggatgat tgggctggga gattcgattg
     3301 ggtgaaaagt gattgtgaag ttatttatct gcctcgaaca ccatctatat cgactactga
     3361 aattatagaa gtcgtcaagt ctattaggta gggcaggtag attgaaactt aagcagaatg
     3421 tattgtttgg taatattgcg gcgctggcga tattacaatt agctacatat atcacgcctt
     3481 tgttaattgt cccttatctt actcgagtgt taggtccggg aggctatggt actgttgcaa
     3541 tttgtttgtc aataattcag atgtcactaa tattgactga ctttggtttt aatcttagtt
     3601 ctacagaata tatagcaaga aattgtcaat ctaaagataa aataaataaa catattggtg
     3661 cagtttttca aattaagata ttattgattt ttctgtgtgt gatttttctt tattttttag
     3721 taaatgacca attgcaacaa tttaaaatag agtttttctt ttattcatgc attgctatta
     3781 ttgggcaaac atttcaatcc ctatggtttt tccaaggggt agaaaagatg aagcggatta
     3841 cacttttttt aactatatca aagttttcat atgttatcct gacttttgta attgtaaaaa
     3901 aaccagagca aggacgattt gtcattcttg cttatgcatt atctcaagtc attggcgcat
     3961 tattatctca atattacatg cgtaaagaag gttataaata tacccctgca aatttatcca
     4021 gcatgttggg ggttttacgt accagtgcat tattcttttg gtcccgactt gctgtctcca
     4081 catatacaac tgcgagcaca ttggtcgtag gagctgcagg acatgtgcaa gctggttatt
     4141 atgctgccag cgaacaactg tataaggcag gacaaaatat aacgacccca gtcagccagg
     4201 cattatatcc ttatatgaca cgggcaaaaa attgggccct gttttttaaa atgatgtcta
     4261 ttcttacggt tgtgatgatt accgctagcc taactgctat ttggttaagt cagtggctga
     4321 ttgctttaat ctttggtcca gaatatatag ttagccagaa tgtattcata gttttcatgc
     4381 ttgccctaat agtcacattt gtaagtacaa attttgggta ccctgtattt tccgcgcttg
     4441 ggaaagttaa agttgcaaat tacactgtta ttttcggtgc tttaacgcat gtagttattc
     4501 tgggtgtttt gttttgtttg ggtgaaataa gtgcctataa cgtagttagg gctgtattat
     4561 ttacagaaat agtcatattg atattgcgaa tgttcttctt tgtgaagtta tacaggcgag
     4621 aagtaaaatg ataattaaaa aagttatatc caaattattt ggcttatttt tttgtgcgct
     4681 ggattacgtt tggaaaaaac aagatataaa tatatttgga gcaggtagag gctctcgtta
     4741 ttttgataac tcgaaaacac tctttgagta ttatatatct caaggtgatc gagcctactg
     4801 gttctacaca ggaaagaagc cagatgttgt tggaatctct cctgatgcat ttgtacaggc
     4861 taacagtttg tatggaatgt tgattgcatt acgtgcaaaa aaagcattcg catgttacgg
     4921 ttctttagat ttttggctat ataggttttc tcgtaaaaca gtatccgttc aattatggca
     4981 tggcataccc atcaaaaagg ttttctttgg tgagaaaaat ttaacgcagc gtaaaaaact
     5041 tcagtattta tttgagattt cagcttttca gtatttcata gtatcctcaa aacttgaaca
     5101 gtatattatt tcagctcaaa ccgggatgca tctggatcga gttaaggtct taggttatcc
     5161 caggaatgat aatttattta aatcagcaaa tgaagtgatt atccctgaag tagttgcatt
     5221 gaacagtaat ttcaaaaaag ttgttttatt cgcaccaaca tttaggaata acgattattc
     5281 tgtatttagt tcatttaatg atgatgaatg gaatgttttt ttcgatttct taaaaacaga
     5341 gaattttctt tgtatctttc gagcgcatgc tgttgagttt ctccaaaata atgatgataa
     5401 cgctaaaaga ttgtctaaat actcaagtaa tgtcattttt cttgggcaag acaaatatca
     5461 ggatgcacag gatttaatgg tttacagcga tattctgatt agtgactatt ccggtatgat
     5521 tccagattac ctgctactgg ataaacctat catcagattt atttttgatg agcagcagta
     5581 tgataacgag cgtggcgtta actttttgca aaaaaatctg cctataggct cggccgcgta
     5641 caatatgccg acattaatag aagcattgag acaatgtgta gaaagcacag aagagtcggt
     5701 ggcagtaaat gcgtttgcaa aaaaaatgta tcacgataat ttatgtggga aatattcaga
     5761 aaaaatatat aactatttta attctttatc acaggggaag gtatgaaaca agcaagaata
     5821 aagcctttct catatttatc attatctttt tttgtgttgt tcttaatttt tttaaatatt
     5881 tcagcagttt cattcgatga gtatctgaca gaccttcaat attcatacgc ctatgtgctg
     5941 tttgctatat ctcttgtggc ggtctactac gctcaaggtg agtcttatac gagtcttggt
     6001 ttcttgttta atacctgttt atttttgttc ataggcggtc gttttattgc gacaacgttt
     6061 ggaatagaca gtgagtcggt gtttacttta gatttcatga ctacatatat accaacatcg
     6121 gatgagaaat tagaaatata cacaagagta gtatcatttc aaatattttt aaatttgggc
     6181 tatttgtttt tacgtaagga agatagttta ttaaatctga ataaaaatga caattcactt
     6241 ccgggtcgtc ttttattttt aattgttata gtgctgtttt tattatgcct tgtggaacaa
     6301 tactcaatgc tgtcgaaagc tatgtctgag gggtacctgg ccagatatgg agatcaagcc
     6361 gaaagttatg atgcaggggc tgcatttaaa tatacgctgc tttttgtttt tacaggctta
     6421 ttatttgcta catgtaaaaa gaagtacatt attataaatt tcatgattta tatgaccctg
     6481 gctactatca ttatactaat tggtgcacgg ggagcatttg tatcaaccat actaatgggg
     6541 gtatggttat acggtttaaa taagaaaata agtttaaaga aactttccat tattttcttg
     6601 tcgttggccg gaatactttt aattatcatg aatttttctg cacgtggcaa ttattattca
     6661 aatgaactca tcgattcttt attgcgtttc ctctatgatc aaggtaatac acttgctgtg
     6721 gtgggttatg taagaaccat taccgattat ccagtgttcg catatatttc aacatttttg
     6781 cctggaagtt cattctttgc atcaatgttt catggtggaa gtgatttaaa tagctacatg
     6841 ctaagttttc ccgcttatgc aaactatgtt gctaatccaa atatgtattt tgctggatac
     6901 ggcctgggat ggtcaatcat cagttctgtg gaaatgttct ccgccgatat cttattcatt
     6961 ttttgcattt tttcggtcct tattggtatg tttttaaagt ttgctaatga acaggcaaaa
     7021 aggaatagtt tctggtttgg ggctgttgcg tgtttgtgtg cgaaaatttt atttacagct
     7081 cgtgctgata tcaaaacgat aattccacta tttatgtatt atgcaataat gtatctaatt
     7141 ataagcactg ctatgcgaat tttgaggcaa agatatggat aatagggatg taatgttttc
     7201 ggttatagtt ccatgctata acgtggtttc gcatataaat gagactgtag cttcacttaa
     7261 taaagctatc gattcggcct gtaccacggt cgaagtcatt tttgttaatg atggctctac
     7321 agataaaaca ttggatgttt taaacgaaat ctgcagtaac aaaccatcat atcgtgttat
     7381 tgatcaggaa aatggtggtg tctcaaacgc aaggaataca ggaataagag agtctacagg
     7441 gaaatatatt ctctttctgg attcagatga tacatacaaa aataatatat tttcgatttt
     7501 atcgaacata tcattttcag atgatattgt tttctttagc tatgaaaaga tagaccagag
     7561 cggtaatacg agggtttata ctattccaaa ctcttatatg gccgagaatt caattactgt
     7621 attaaaagat ctttttagta aaaaaatcta tttaaatatt tgtgcgctag ctgtaaatag
     7681 ggattgtata tttcagaata atattttttt cgatgaaagt attcaacatt gtgaggatct
     7741 tcaattcatc attaaaatga ttacttattc aaaatcattc caattcataa atgaaccttt
     7801 atttacctat aattacactc ccggttctgc tgttaatagc aaggtagatg agaaacatta
     7861 ttcaaaattt attgcctttg agaacatgcg cgatgttttt ataaaaaatg ataaatataa
     7921 agagttaatc ccggcgtacg atttttacgt tgcaacagta tacttattac tacttaaaac
     7981 gattgtctca aatggtgtgg ttaacaacaa acaactaagt aagttcgttg aatattcttt
     8041 tatcctcaag agaaaaatgg aattaccacc aaatacaatg ggtgcttttg ttttgatatt
     8101 tacaataatt aattatattt ttcctatgaa agtaaaattt ttaatgatgt catttatctt
     8161 acggatgaat aaaggtttaa gatgagtatt acaaataaaa caattgcgct ggtcattgta
     8221 acatataata gatgtaacct tttgatggaa atgctttcct ctatcgaaaa catgagtgtt
     8281 aagcctgata ttgtctatgt tatagacaat aacagtagtg ataatacttc atcggttgtc
     8341 acagagtgtg attcaagaaa aaatattaat ataaaatatc acaacactgg atataacgct
     8401 ggaggtgctg gcggctttta tatcggcagt aaaatggctt atgaagatgg ttgggataga
     8461 atttggcttg cggatgatga cattgttttg gataaggagt gtcttagtaa cgcaatggag
     8521 tatgatgatg gacgcactat cttacaacct atgcgttata atatggacgg atcctgtgcc
     8581 gaaatttcag ctatccagta cgatttaagt aatccttttt atttacgtcc aaaacgcaag
     8641 acggttcaga atattttcaa caaaaatatt ctgtcatatg atatccaatc cattccgttt
     8701 gaaggaccga ttattcccag ggaagtattt aatgtcatag gttttccaga tgaacgtttc
     8761 tttatattta atgacgattt agattttgcg attagagctc agagagctgg cttcagcata
     8821 aaatgtatta ctaacgcaaa aatcgtacgt aaaattccct ttgtacagtc ggtagcatta
     8881 aaaacatgga agggctattt catgtttaga aattattttc gggttcagaa agtttatggt
     8941 tcaagcccac ttatttatct gagaattttg ctcgtgttct gccttgcact gggacattca
     9001 ctcgtgcgta tggatatcaa ttcaattaag atgctgtgcg gtgcgttgaa agatggtctc
     9061 tctcaagaat ttaagctgac tgagaaatac aaaccatgag tactaccgat agccctaata
     9121 taaaaacatc aaataacaat tttgatatag taaggctcat tcttgcgtta gttgttgttt
     9181 tctcacatat aagagattta aataatatta attcccggtt cttctccatt ttctcaggtg
     9241 actttgccgt agaatgcttt ttcgtaatta gcggttattt gatcattaga agctttaaaa
     9301 ataatcaaaa tatcagaaaa tatatttttt cgagggggat gagaattctc cctatgtact
     9361 acgtttctat agtatccttt ttcgttatat tattttttat ctctcacctt tccgttgttg
     9421 aatatttaaa ggaaggcgga attcgatatt tagtatttaa tggagtattc ctcaatttca
     9481 tccagccttc gctgccggga gtgttcgttg ataatcctcg aatctctgcg gttaatgggg
     9541 ctctttggac tataaaagtt gagatcctat tctatatatt tgttccattc tttgtcctgt
     9601 tcattaaaaa aatcaatcga catattattc ctatatttat aattcttatt ttatcgtcta
     9661 ttgtattcaa gtggattgtt acagactatc atcaaatttt acatattcct gagcaactgg
     9721 ttaatcagtt accgtcggtg ctaagttatt ttctattagg cggcttggtt aattatctgg
     9781 atctatctaa gatatcaaaa aaaacagtat tttggttttt tatagtatca gctttttatc
     9841 ttttactaag tagtaaaacg tcattggatt tacttgttcg cccatttgtt attgcagggt
     9901 tcatactgcc gatatgtttg cagcgtaaat ttttaataag tgtgcctgaa aaaatgggtg
     9961 acctttcgta tggtatatat gtaacacatt tccctcttgt tcaattacta attgcagtag
    10021 ggttgtataa ttcattttgg ttaggttttt gtttaacatt tactagtctt tttattttgt
    10081 cttttattag ttggcatctt attgagaagc cagctcttcg ttttgctaaa tctgcaggaa
    10141 aaaataaagt tgtgaggggt gtatgagaat tgctattgtt ggtgctggat ttagtggtgc
    10201 agttatagcc agggaattgg ctgaacatgg tcactcagta gatatctttg agaaaagaga
    10261 ccatgttgct ggtaattgtt atagtaaaag agacccagac acaaatgtta tggttcatgt
    10321 ttatggcccg catattttcc atacggatga taaagaagta tgggaatata tcaataaatt
    10381 cggaatgttc gagccatatg ttaatagagt caaggccata actaaaggcg cggtttattc
    10441 cctgccaatc aatctccata caattaacca gtttttccac aaaactctga accctgctga
    10501 agcgaaagac ttcataaatt ctttgtgtga actattcgaa tttgagccga ttacattcga
    10561 gcagcaggct ttatcattta ttggtcgaga cttgtacgag gcatttttta gaacgtatac
    10621 cattaaacaa tggggtcttg aaccttcaga gcttcctgcc agtattttga aacggctacc
    10681 agttagattt aattataacg ataactattt cagccatcgt tatcagggta tgccacgtga
    10741 tggatatact aatataatta gtgccatttt ggaccatgca agtatcgctg tgaaattaaa
    10801 cactgatttc aaaaaaaatg tcggcaatga atatgatcac gtgttttata gcggcacgat
    10861 tgacggttat tttgactact cccatggccg ccttccttac aggactctag attttgtcac
    10921 ggaaagacat aaaggtgatt atcaaggaac cgctgtaatt aactattgtg atgatagtga
    10981 gaaatttact cgtattactg aacacaaata tttttcccct tgggaagagc acagcaatac
    11041 aataatctat aaagaataca gccggacatg tactgagact gattctccct actatccaat
    11101 tcggcaggtt caggaaaaag aattactaag aaagtatgtt gatttagcaa atcaaacaaa
    11161 caatgtttca tttattgggc gtttaggtac gtacagatat cttgacatgg atgtaactat
    11221 tcgtgaggca ctaaatgctg taaaactctt tatgaaaata aaaaaggaga acgaaaaagt
    11281 tccagcattt ttcacggaaa tactgtgaag atataatttc tgctccgaga tacataatgt
    11341 cgatagtatt agtaatggtg agcattgtta tttattgtca gtttgacagc actcttggct
    11401 atccagaatt gttggatatg agttaatatc acaccacatt caagccgcgc atatctcgcg
    11461 gtgaccacac ctgacaggag tatgtaatgt ctaagcaaca gatcggcgta gtcggtatgg
    11521 cagtgatggg gcgcaacctg gcgctcaaca tcgaaagccg tggttatacc gtctccatct
    11581 tcaaccgctc ccgtgaaaag acagaagaag ttgttgccga aaatccaggc aagaaactgg
    11641 ttcctcatta cacggtaaaa gaattcgtcg aatctcttga aaccccgcgt cgtatcctgt
    11701 tgatggtgaa agccggtgcg ggtaccgatg ccgctattga ttctctgaaa ccgtatctgg
    11761 ataaaggtga catcatcatt gatggtggta atactttctt ccaggacacc attcgtcgta
    11821 atcgcgagct gtcggctgaa ggctttaact tcatcggtac cggtgtttcc ggcggtgaag
    11881 agggcgcgct gaaaggccca tctatcatgc ctggtggtca gaaagaagcc tatgagctgg
    11941 ttgccccaat cctgaccaaa atcgctgctg ttgcggaaga tggcgaaccg tgcgtgacct
    12001 atattggtgc cgatggtgcg ggtcactatg tgaaaatggt tcacaacggt atcgaatacg
    12061 gcgacatgca gctgatcgca gaagcgtact ctctgctgaa aggtggcctg aacctctcta
    12121 acgacgagct ggcgactatc ttcaccgagt ggaacgaagg cgaactgagc agctatctga
    12181 tcgacatcac caaagacatc ttcaccaaga aagatgaaga cggtaattat ctggttgatg
    12241 tgattctgga tgaagccgct aacaaaggta ccggtaaatg gaccagccag agctccctgg
    12301 atctcggcga gccactgtcc ctgatcaccg agtctgtatt cgcgcgttat atctcctctc
    12361 tgaaagatca gcgtgttgca gcgtctaaag tgctttctgg cccgaaagcg gaactggctg
    12421 gcgacaaagc ggctttcgtt gagaaagttc gccgcgcgct gtatctcggt aaaatcgttt
    12481 cttacgcgca gggcttctct cagctgcgtg ccgcgtctga cgaatacaac tgggatctga
    12541 actacggtga aatcgcgaag atcttccgtg cgggctgtat tattcgtgca cagttcctgc
    12601 agaagatcac cgatgcctat gctgaaaata gcggtatcgc aaacctgctg ctggcaccgt
    12661 acttcaagaa tatcgctgat gagtatcagc aggcgctgcg tgacgtggtt gcctacgctg
    12721 tacagaacgg tattccggtt ccgaccttcg gcgctgcggt tgcctactat gacagctacc
    12781 gttcagcggt actgccggct aacctgattc aggctcagcg tgactacttc ggtgcacata
    12841 cctataaacg tactgataaa gaaggcgtat tccataccga atggttggac taatcctaaa
    12901 taaataatgg aagcccggcg atgccgggct tttttttgcg ctcaattctt attgcattat
    12961 ggtcctgtct taatattaac ttaatatcga tggtttattc tctctgaaga aagtccgaat
    13021 tgtttgtaat tctattgcag tgaatgcgct tacgagttaa aaaaatttta aacgtattga
    13081 tgtgatatca cattataaat aattaagttt attctgagag agtacaatga aagttaccat
    13141 ctctggtaca ggctatgtcg gcctctcgaa cggcctgctg atcgcgcaac accacgacgt
    13201 cgtggcgttg gacatcattc cttcacgtgt ggatatgctc aacgcgcgtg tatcacccat
    13261 tgttgacaaa gagatccaac agtacctgag ctcaggcgag ttgcgtttct ccgcaactac
    13321 tgacaaatac ctggcttatc agaatgctga ttacgttatt atcgcgacgc caacggatta
    13381 cgaccctaaa actaactatt ttaatacgtc cagcgttgag tcggttattc aggatgtcat
    13441 tgatattaat ccgcaggccg ttatgattat caaatcaacg gtgccggttg gttttacgga
    13501 ttcggtgaag aaaaaatatg ccactgataa tattattttc tcgccagagt ttttgcgtga
    13561 aggtaaagcg ttgtacgaca accttcaccc ctcgcggatc gtgattggtg agcgttcaga
    13621 gcgcgcagaa cgttttgccc gaatgctcca gcagggcgct attaagcaag acatacctac
    13681 gctgtttacc gattcgacgg aagcagaggc gattaaactc tttgccaaca cctatctggc
    13741 aatgcgtgtt gcttacttta acgagctgga tagctacgcg gaaacgttgg ggctcaattc
    13801 ccgccaaatc atcgaaggtg tctgtctgga tcctcgcatc ggtgctcact acaacaaccc
    13861 ttcctttggt tatggtggct attgcctgcc gaaggatact aagcaactgc tggcgaacta
    13921 ccagtcggtg cctaacaaca ttatctctgc cattgtcgat gcgaaccgga cgcgcaaaga
    13981 ttttatcgct gaggcgattc tggcgcgtaa gccgaaagtg gtcggtatct atcgcctgat
    14041 tatgaaaagc ggctccgata atttccgtgc atcctccgtg cagggcatca tgaaacgcct
    14101 gaaagcgaag gggataacgg tgattgtgta cgaaccggtg atgaaagagg atgaattctt
    14161 cagctcacgt gtcgttcgcg atctggatgc cttcaagcaa gaagcggatg ttatcgtttc
    14221 taaccgtatg gccgaggagt taaccgacgt agcagaaaaa atctacacgc gcgatctttt
    14281 tggcaacgac taatctaaat ttttgacaag ctgaatgcct gacaagacgg gcgttcagcc
    14341 ttttttctga ttcttttcag gtttctctgt ttttggtgaa aatcctatag aaattcggat
    14401 tggtctaggt aaactgatgc cctcgtgggt atttcagttt ggatgtcact aaggtttata
    14461 tgattcaaga taacaataat gggcgcagcg ggtatgagcg tgagccggag cagatcgacc
    14521 ttatcgattt gttgatgcaa ctctggcgtg gcaagttgac catcatcgta tgcgtcatcg
    14581 tcgcgattgc gctcgctgtg gcttatgtct ttttagcgaa agagaaatgg acctctaccg
    14641 cgaccatcac tcgcccggat gcggcgcaga ttgcgaccta ccataacgcc ataaatactc
    14701 tggattcatc gattaccttg tccgacgttc aaaaccaggt gattaaccgc tttaactctg
    14761 catttatcgc attgacggaa gcgttggata atcagcaaga gcctgagaaa ctgactattg
    14821 agccctctgt taagggccag aatctacccc taactgtgac ttacgtgagt gatacagcac
    14881 aatccgccca gcgcggtgta gctgaataca tccagaaaat cgacgaacaa atcgctgatg
    14941 agctggaact tgaccttaat gacaccatca aattacgtgt gatgaacatt aaggacgacc
    15001 tggaaaacca ggaaaccgta gctcaggaac aaaaagacct gcgtatcaag cagatcactg
    15061 aatccctgaa atacgcgcag aaaagtaacg tcaccaaacc gcagcttcag cagacccaag
    15121 acgtaactca ggaaaccatg ttcctgttgg gcacagaggc tctgggcgca atggttgcca
    15181 acgaagccag ccgtccgctg acctttggtg cgggctacta cggtttgaag aaaaacctgc
    15241 ttgatatcga aaaactgaaa gtcgatccga aaaccattca cgtctatcgc tacgtgatga
    15301 agccgacgct gccaatccgt cgcgacagcc cgaagcgtgg tctgacgctg gtgctggcgg
    15361 tgctgctggg cgggatgatt ggtgccggtg ttgtgttggg ccgcaacgcg ttgcgtaact
    15421 acaaaccgaa agcataacgc ttacgtcaga tagcaaaaaa ccgggctcgc ccggtttttt
    15481 tacgcctgtt gaaaatttac tgatgacgtt ttcgcaggtt ctcaatcacg gtcgttaaat
    15541 cgagatcctg atcctgcagc agcaccagca ggtggtacat caagtccgac gcttcattgg
    15601 tcagctcaaa gcggtctttc accgttgctg ccagtgcggt ttctacgcct tcttcaccca
    15661 ctttctgcgc gatacgcttg gtgccgctgg catacaggcg cgcggtgtag gagctttccg
    15721 ggtctgcggt tttacgctcg gccagcagtt gttccagttg atacaggaac aaccatt
//
