LOCUS       EU694097               14049 bp    DNA     linear   BCT 22-SEP-2008
DEFINITION  Escherichia coli serogroup O3 O antigen gene cluster, complete
            sequence.
ACCESSION   EU694097
VERSION     EU694097.1  GI:203285031
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14049)
  AUTHORS   Ren,Y., Liu,B., Cheng,J., Liu,F., Feng,L. and Wang,L.
  TITLE     Characterization of Escherichia coli O3 and O21 O antigen gene
            clusters and development of serogroup-specific PCR assays
  JOURNAL   J. Microbiol. Methods 75 (2), 329-334 (2008)
   PUBMED   18700154
REFERENCE   2  (bases 1 to 14049)
  AUTHORS   Ren,Y., Liu,B., Cheng,J., Liu,F., Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2008) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P. R. China
FEATURES             Location/Qualifiers
     source          1..14049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O3"
     misc_feature    1..14049
                     /note="contains O antigen gene cluster; also contains galF
                     and gnd"
     mobile_element  1155..2412
                     /note="remnant"
                     /mobile_element_type="insertion sequence:H-repeat"
     gene            2537..3700
                     /gene="wdaW"
     CDS             2537..3700
                     /gene="wclR"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACH97149.1"
                     /db_xref="GI:203285032"
                     /translation="MKSTHSKIKIAHVQLMPLLSGVQRVSLQEFELLPNEQFDINLIC
                     KESGPLTDYLDDSVRAFFVPTLCRNISLIKDMKSLISLYKLLKKEKYDIVHTHSSKTG
                     ILGRIAARLAGVPCVVHTVHGFAFESTKRKSVKLVYKWLEIFAAKCTTRLICLHNEDK
                     EICIKELYVDPMKISVIPNGVDLEKFAPAINKGDLKEKILGLKRNSFVFTMVGRLWPQ
                     KNPLYFAEAAKYIIENNLIPDSVFVIVGDGELMNDLKYNYQTDMNLKKRLLLLGWRND
                     IPNILKASDVFVLPSLWEGMPLAILEAQSTGLPCIVSNINGNNCLVKNEFDGFLIELN
                     DIDSFINALVRVTDDKVLNIMSKNCRNKIVNGFNIVKRVDKIKDLYIDMVVNK"
     mobile_element  3977..4253
                     /note="remnant"
                     /mobile_element_type="insertion sequence:H-repeat"
     gene            4566..6059
                     /gene="wzx"
     CDS             4566..6059
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACH97150.1"
                     /db_xref="GI:203285033"
                     /translation="MHVRVAVNFASKITFALISIITIPVIKSKLGVNAYGVLTFFLSI
                     QVVILLLEGGMSATMARGLLNKKYKIYNISKAEYQSVYFIFFILVSLFVFSIFIVFNH
                     NISTLIYNLDDREVYSFISADYVILLAGAMIALQFYIIYYEALFVAYEKQVSYGIHII
                     VFNVIKTIISLFLLIVFNLGLDVYFLVHVFCTFILIILLHIKAIKNGLLEITTLRVKK
                     IYTKISFMKEEFHFSKNILCVSILSAIAFQADRLFITKYMGISAVGMYGIAYTLSTVP
                     TLFTSSLYNVIYPRLIILTREQSSSAISFFELVSKLIYIIIIPTCVIISLNSTQILNI
                     WIGKHEGIISLLLSILIIATLFQGLQVIPFALQMAQERLKFTVFMNAIFVPSLILAYY
                     LVSIHKILLYISIVWSVYNIITFFSLTIYLYAKNKMYTRLLILYFNICLCAVVTVVIC
                     SIFKNQESQIFLSLVNISLQFFMSMSLCIILLFWKTIYGALRCVCCR"
     gene            6041..7210
                     /gene="weiN"
     CDS             6041..7210
                     /gene="wclS"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACH97151.1"
                     /db_xref="GI:203285034"
                     /translation="MCLLSVVIATHNRAGYAKKSIESILSIDVEEFQLIIHDTSDNNV
                     LEEFCAEIQDARLKYIHCNELLSMTENFNRALEYVQGEYTIMIGDDDTILSTAIKYCR
                     YAKEKKIDVISQKISAEYCWPDFRSKISGDFNASSLIVNKFTSNQVTYEGRKQYYEAV
                     KNCFQGTASLPKLYHGIVKTSLLLKIKENTGTYFHGVSPDISIALSLAYNCERYIEVD
                     YPITLPGSSGGSNAGRSAMRTHVGKLDDDPHMKRFKSYVWPNLLPRYFSVETVWAQAG
                     LVTIQKIDNNKGFNFIKFYALCLIKHRPYKKFIIDEIKKYKKVKGGFNCIIYTKIIYH
                     MSYFYINGISNLSKKIIKYVFKKLKSQEKQKNIKANDIFEARMIIEKELSKETFE"
     gene            7207..8292
                     /gene="wzy"
     CDS             7207..8292
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACH97152.1"
                     /db_xref="GI:203285035"
                     /translation="MIIFSTFLLFYFFIFLSLLPVLIKEGARGNGKYFYFFDLYIYLV
                     LASLIIYIICTRDIFSDSDTERYLSILEYYRINGFYLFDKNYIFAVYTYLYSLYIDGY
                     YMYMIIPLITLLYSYCKLVKPLKYPVYSFSLVLIATFPVFWQLSASAFKQCIAISFIL
                     LALVHIIQTKYKQAVLLSLIASSFHFTALTFFIFSLFFYIRKERVSLNLFFLIWFFSV
                     IFSISGLNSYIPSIIPFYDLVGESKVNIYLESTNNATGFRVDFFLFSLAPVLIIFINR
                     TLVIKDRYIKMIALSYLYFNSIYNIMSFIPFSDRVAAYSWCLSPIILLWSALKCRSSI
                     YMLSSIGIALLNPLIFMYYNSMFIFLK"
     gene            8299..9312
                     /gene="fnlA"
     CDS             8299..9312
                     /gene="fnlA"
                     /note="UDP-D-GlcNAc 4,6-dehydratase, 3-and 5-epimerizase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlA"
                     /protein_id="ACH97153.1"
                     /db_xref="GI:203285036"
                     /translation="MFNGKKILITGGTGSFGNAVLRRFLETDIQEVIIFSRDEKKQDE
                     MRTLYRNDKMKFIIGDIRDYTSVLAATSDVDYIFHAAALKQVPSCEFYPWEAVKTNII
                     GTENLLNAAKQNKVKKTICLSTDKAVYPINAMGISKAMMEKVMTAKARTFNNEDSVVC
                     GTRYGNVMASRGSVIPLFIQQIQTNQPLTITDPEMTRFMMTLDDAVDLVLFAFNNGQN
                     GDIFVQKAPAATIAVLAKALVELMGVPNHPIKVVGSRHGEKIYETLLSREEMAKAIDH
                     GDYYQIQPDNRDLNYDKYLSEGSKKIELFDDYNSHNTYRLNVDEMKKLLLKLNIIQNV
                     LNS"
     gene            9325..10194
                     /gene="qnlA"
     CDS             9325..10194
                     /gene="qnlA"
                     /note="UDP-2-acetamido-2,6-dideoxy-L-lyxo-4-hexulose
                     C4-reductase"
                     /codon_start=1
                     /transl_table=11
                     /product="QnlA"
                     /protein_id="ACH97154.1"
                     /db_xref="GI:203285037"
                     /translation="MQKILILGVSGMLGHTLFRFLSSQQDLLVTGTVRHITQEIKLVS
                     SKNIVLMRDVFNKEKLENIINSNDVIINCIGAIKQKYDNKNTDYILLNSYFPHLLNEI
                     CIESNKRLIHFSTDCIFNGEKGNYDDGSLSNVIDMYGKSKYLGEVYGDKTLTLRTSLI
                     GHELKSSYSLIDWFLNSNSTVNGYTKAIFSGLPTIEIAHFLYEHVLQSQLHGIYNLSA
                     APISKFDLLTLVNEVYKLNKTIVPKSDFVIDRSLDSHRLRSLTKYTPPDWNTLVVKMY
                     LDYLSLGALLNER"
     gene            10184..10651
                     /gene="wclT"
     CDS             10184..10651
                     /gene="wclT"
                     /codon_start=1
                     /transl_table=11
                     /product="WclT"
                     /protein_id="ACH97155.1"
                     /db_xref="GI:203285038"
                     /translation="MKDNLSFSKLISEAEINSRNRSHLNLHESFSDPIQKTLICCCSN
                     TYIPPHYHKFAHQSELFIRLKGCFSLLIFSVDGVLTRRITIDEENPLYEIKPGVIHTV
                     VALDRHNILLEIKRGPYVESEAKNFPDWVTLENSDNQLDLERLRTLKVGDKLS"
     gene            10686..11483
                     /gene="wclU"
     CDS             10686..11483
                     /gene="wclU"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACH97156.1"
                     /db_xref="GI:203285039"
                     /translation="MLPLISIITPTYNRAYTLDRLYNSLVTQDSEKFEWIIIDDGSTD
                     ETRKKCLGYINDSIFKIQYYKQNNSGKPAAINLGVTKAVGDYIFIVDSDDALTLNAIG
                     TIYDCIVKYTVASDFEFSGLGFRKSFFDGKMLGKHIERNTNSPMLLHSTDCNNLFKVD
                     LAYCFKRELMIKYPFPKFKDEKFVPELFIWNKITDVAKIIFFPNVVVYYCEYLADGLT
                     NNFKKQLRHNPIGFAVYYKDQFHREHSIWGKIKMAIRYIQCIIYKFI"
     gene            11486..12604
                     /gene="wdaZ"
     CDS             11486..12604
                     /gene="wclV"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACH97157.1"
                     /db_xref="GI:203285040"
                     /translation="MQGSSIHLISCYDFYMKILHVVPQLKHGGVETAVFNSIERIRKS
                     GYDFNVLTIERGNCFNNSYVKVEHCLNSSVYNPVCFYKFIRFIHKEKPDLIVYSLWKS
                     ALLGISLCLLKPFLKSKPKTALIIHNTRFAHFVDYIITKLSLKITDSIYFDSEKSKAY
                     FLNRDYATGEVISFIDKKMVAKKFNNINGEIKFVFIGRLHSQKGVDRALKYIHLFKKQ
                     HEEIRFDIYGPDEGELNNIINKVNKLGLQDKVVYKGVVDNNKVNDILTQYDFYLQFSH
                     FEGMAMSVVDAMQVGVIPIVNNVGEIPNYVKNNINGIILDDKKLGDENYLNLNISCLL
                     NIIKNDGGYFLYNNAIKTFQDNNTYVDDYIKKIINANN"
BASE COUNT     4594 a   2132 c   2710 g   4613 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 caactgctgg cggaagtaca atctatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggtgaac ctttaggttt aggccactcc attttgtgtg cacgccccgc cattggcgac
      241 aacccatttg tcgtggtgct gccggacgtt gttatcgatg atgccagcgc cgacccgttg
      301 cgttacaacc ttgctgccat gattgcgcgc ttcaatgaaa cgggacgcag tcaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca tccagaccaa agaaccactg
      421 gatcgcgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgttggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcacgcagcc aggtgcatgg gggcgtattc agctgactga tgccatcgct
      601 gaactggcga aaaaacagtc cgttgacgcc atgctgatga ctggggacag ttacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttc gtgaagtatg gactacgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ctgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcgaa agtaatttgt
      841 tgcgaatttt cctgccgttg ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgctacatc gtaggcatgc
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tatctagggg aagatcatcg
     1081 tgtgcggtaa gcacaaaaat gcaaaaagga acgttttgca tgtaataagc ataagcttgt
     1141 aaagagatca gggaacaggg aaagatcaca taacgccggt tttttgtact aaataattcg
     1201 cactaaatgt tcaataattg agacattcct tattgtctaa aactgttttt cacagcttat
     1261 atatgatcaa atactcctta cataaataag gagaacaaaa tggaacttaa aaaactgatg
     1321 gaacatattt ctattatccc cgattacaga caagcctgga aagtagaata taaattttca
     1381 gatactctac tattgactat ttgtgctgtc atttctggtg cagaaggttg ggaagatata
     1441 gaggattttg gtgaaacgca tctcgatttt ttgaagcaat atggtgattt tgaaaatggt
     1501 attcctgtcc atgataccat tgccggagtt gtatcctgta ttagtcctgc aaaatttcac
     1561 gagtgcttta ttaactggat gcgtgattgc cattgttcag attataaaga cgtcattgca
     1621 attgatggga aaacgctccg gcactcttat gataagagtc gtcgcagggg agcgattcat
     1681 gtcattagtg tgtcctcaac aatgcacagt ctggttctcg gacagatcaa gacggatgaa
     1741 aaatctaatg agattacagc tattcctgaa cttcttaaca tgatggatat taaaggaaaa
     1801 atcatcacaa cggatgcgat ggggtgccag aaagatattg cagaaaaaat acaaaaacag
     1861 ggaggtgatt atttattcgc tgtaaaggga aatcaggggc gactaaataa agccttcgag
     1921 gaaaaatttc cgctgaaaga attaaataat ccaaagtatg acagttacgc aattagtgaa
     1981 aagaatcacg gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt
     2041 attgatttca cgtttgaatg gaaaggactg aaaaaaatta tgcgtggcag tctcctttcg
     2101 gtcaataata gcagaacaaa agaaagagcc agaaatgacg gtcagatatt atatcagttc
     2161 tgctgattta accgctgaga agtttgcgac agcgattcga aatcactggc acgtggagaa
     2221 taagttgctc tggcgtctgg atgtagtaat gaatgaaaga cgactgcaaa ataggaagag
     2281 gaaacgttca gggataagac atattgcaat taatattttg acgaaagata aggtattcaa
     2341 gcacggttaa gacgttcgat gaagacagcg actacctgcg tcagtctttg tgaggagcgg
     2401 actctagtaa tccaactttg ttgttaggtt ttactcgcgg aataatcatg ggcgaatttt
     2461 attaataatg tatattttcc taattaaaaa catgttaaga aaagaaacaa aactttcaag
     2521 ctaggttact tatgatatga agtcaacaca ttctaaaatt aaaattgcac atgttcaatt
     2581 gatgccacta ctgagtggtg ttcagcgagt atcgttacaa gaattcgaat tattaccaaa
     2641 tgaacagttc gacattaatt taatttgcaa agagagtgga ccattaactg attatttaga
     2701 tgatagtgta agggcttttt ttgtcccgac attatgtaga aatatatcgc tcataaagga
     2761 tatgaaatca ttaatatctt tatataaatt attgaaaaaa gaaaaatatg atatcgtcca
     2821 tacacactcc tctaaaactg gtattctagg gcggatagct gcgcggctgg caggagttcc
     2881 ttgtgtagta catactgtac atggctttgc ctttgaaagc acaaaaagaa aatcagtaaa
     2941 gctcgtttat aaatggctgg aaatatttgc agccaaatgc actacccgat taatttgtct
     3001 tcataatgaa gataaagaaa tatgtataaa agaactatat gttgatccaa tgaaaatttc
     3061 tgtaattcca aatggagttg atttggaaaa attcgctcca gcaattaata aaggagactt
     3121 aaaagaaaaa atattagggt taaaaagaaa tagctttgta tttaccatgg tgggacggtt
     3181 gtggccacaa aaaaatccgt tatattttgc tgaagcagca aagtatatta tagaaaataa
     3241 cttaatacct gattcagttt ttgtcatagt tggtgatggg gagttgatga atgatttaaa
     3301 atacaattat caaacagata tgaatttaaa aaaaaggtta ttacttttag gctggcgaaa
     3361 tgatatccca aacatattaa aagcaagtga cgtttttgtt cttccttctt tatgggaagg
     3421 aatgcctctt gccatattag aagctcaatc tacagggtta ccatgtatcg tttctaatat
     3481 caatggtaat aattgtttgg ttaagaatga atttgatgga ttcttaattg aacttaatga
     3541 tattgatagc tttataaatg cgttagttag agtgacagac gataaagtct taaacattat
     3601 gtcaaaaaat tgccgtaata aaattgttaa tggatttaat atagttaaaa gagtggataa
     3661 aattaaagat ctatatattg atatggttgt taataaatga ttgtgtgctt tttgaatgtt
     3721 tatatcaata tcgcactgta tattttattt tatgtagatt tattatgaat ataaaggcat
     3781 ggtgatttac atcattattt atatacagac gctgttattg gtatagagaa atatcatata
     3841 acattagttt tcgtacaaaa tgtaaaaaat gaaagattta ttactaagta agactatttt
     3901 tgataaccta tatattatca aatactctgt acataaagga gggaaatgga acatgaaaaa
     3961 taatagtcgt atttctgtta accaatttca aacaacctgg gaaagtggaa cgtaaattat
     4021 tagatattta ctactgatta tttgctcctg cttcgtcatt tctgacgcag aaggatggga
     4081 gtatacaaag gatgtttgtg agacgtgtgt cgatttcttg aagcaatacg atgatttggg
     4141 acatggtact cctgtttata ataccatagc tgagttctat tccgtatcag tcctattaac
     4201 tggttacgtg atgttattct tcagattata aagacatcat tgcagtaggt ggaacttaat
     4261 agcggagcag aataaagagt ttaaaatgat attcaatatt gtatcagttc tgctggttgt
     4321 ttaaattctg agaagtccgc gatcgtgatt tgaaactgct agaatgttga gaataatcag
     4381 cgctggtgtc tggatgtagt aatgaatgaa gagaagtgct caataagcaa agcagctatg
     4441 gacagtgatt agcccgcatc agttcttgca ggggtgtgtt tttattatct ttccctgatt
     4501 attgggtgac tgtgtgactt atacattctc ttattttgag tgtgatggta aaaatgtttg
     4561 actctatgca tgttcgggtt gctgtaaatt ttgcatcaaa gataacattt gctttgatat
     4621 ctataattac aattccagtt ataaaatcaa aattaggagt aaatgcttat ggtgtcctaa
     4681 cattctttct atcaatccag gtagttatat tactgcttga ggggggaatg agtgccacaa
     4741 tggctagagg actattgaat aaaaaatata agatatacaa tataagtaaa gcagagtacc
     4801 aaagtgttta ttttatcttt tttatattag tgtcattatt tgtattttca atttttattg
     4861 tctttaatca taatatttcc acattgattt acaaccttga tgatagggaa gtctatagtt
     4921 ttattagtgc tgattatgtt attttgctgg ctggtgcaat gattgccttg cagttttata
     4981 tcatatatta tgaagcatta tttgtggctt atgaaaaaca agtctcttat ggaattcata
     5041 taatagtatt taatgtaatc aaaaccataa tatcattgtt cttgttgatt gtctttaatc
     5101 ttgggttgga tgtttatttt ttagttcatg tattttgtac ctttatactt attattttat
     5161 tgcacattaa ggcaataaaa aacggattat tagaaataac aacattgcga gtgaaaaaaa
     5221 tatacacaaa aatatcattt atgaaagaag aatttcattt ttctaaaaat atattatgcg
     5281 tgtccattct ttctgcaatt gcattccaag ctgacagact atttattaca aaatatatgg
     5341 gtattagtgc tgttggaatg tatggtattg cttatacatt atcaacagta ccaactttat
     5401 ttacatcatc attgtataat gttatctatc caagacttat tattttaaca agagagcaaa
     5461 gcagcagtgc gatttcattt tttgaattag tatcgaaatt aatatatatt atcataatac
     5521 ccacttgtgt gattatttcg cttaattcga cccaaatcct aaatatttgg ataggaaagc
     5581 acgaaggaat aatatcattg ctgttaagta ttctaataat agcgacgtta tttcaggggt
     5641 tgcaggtgat tccttttgca ctacagatgg ctcaagaacg actaaaattt acagttttca
     5701 tgaatgctat atttgtgccc agcctaatat tggcatatta tttggttagt attcataaaa
     5761 ttctgttata catctccata gtgtggtcag tatataatat aataactttt ttctctttaa
     5821 ctatttattt atatgctaaa aataaaatgt atacaagact acttatttta tattttaata
     5881 tatgcttgtg tgctgtcgta acagtagtta tatgtagcat ttttaagaat caagaatctc
     5941 aaatattctt gtctctagtt aatatttctc ttcagttttt tatgtcgatg agtttgtgca
     6001 taatactgtt gttctggaaa actatatatg gagcattgag atgtgtttgt tgtcggtagt
     6061 aattgcgact cataatcgcg cgggatatgc taaaaaaagc atagaaagta ttctttcaat
     6121 tgatgttgaa gaatttcaac taattattca tgatacaagc gataataatg tacttgagga
     6181 attttgcgct gaaatccagg atgcgagatt aaaatatatc cattgcaatg aattattgtc
     6241 gatgacagaa aattttaata gagcattaga atatgttcag ggagaatata ctataatgat
     6301 aggagatgat gatacgattt tatcgactgc gataaaatat tgtagatatg cgaaagaaaa
     6361 aaaaattgat gtaatctcgc agaagatatc tgcagagtat tgttggccgg attttagatc
     6421 taagatctca ggggatttta acgcatcgtc tctgatagtg aacaaattta caagtaacca
     6481 agttacttat gaggggcgta aacaatacta tgaagcagtt aaaaactgtt ttcaaggtac
     6541 tgcaagcctc cccaagttat atcatggtat tgttaaaact tctttattgt taaagataaa
     6601 agaaaataca ggtacttact ttcatggggt tagccctgat atttcaattg cattgtcgtt
     6661 agcgtataac tgcgaacgat atattgaagt ggattatcca attacccttc caggatcttc
     6721 ggggggaagt aacgcaggta gaagtgcaat gcgtactcat gttggtaaat tagacgacga
     6781 tccacatatg aaaagattta aaagttatgt ctggccaaac ttattgccaa gatatttcag
     6841 tgtagagact gtgtgggctc aggcaggctt agtaacaatc caaaaaatag ataataataa
     6901 aggatttaat tttattaagt tctatgcatt atgtttgatt aaacatcgac cctataaaaa
     6961 atttattatt gatgagataa aaaaatacaa gaaagtaaaa ggagggttta attgtataat
     7021 ttatacaaaa attatttatc acatgtctta tttttatatt aatggaatat caaatctttc
     7081 taaaaaaatt ataaaatatg tcttcaaaaa acttaagtct caagagaagc aaaaaaatat
     7141 aaaagcaaat gatatttttg aagccaggat gataattgaa aaggaattat caaaggaaac
     7201 atttgaatga ttatattttc tacttttcta cttttctact tttttatttt tctttcattg
     7261 cttcctgttt taattaaaga gggagctaga gggaatggca aatattttta tttctttgat
     7321 ttatatattt acttagtatt agctagtctt attatttata taatttgtac acgagatatt
     7381 ttttctgact ccgatacaga acgttatctt agtatattag aatattatag aataaatggc
     7441 ttttatcttt ttgataagaa ctatattttt gctgtgtata catatttgta ctctctatat
     7501 atagatggtt actatatgta tatgataatc ccacttatta ccctgctata ttcatattgt
     7561 aagttagtaa aaccactaaa atatcctgtt tatagttttt ctttagtatt aatagcaaca
     7621 tttcctgttt tttggcaatt gagcgcaagc gcatttaaac aatgcattgc aatatctttt
     7681 attcttttag cattagtgca tattatacaa acaaaatata aacaagcggt gcttttatct
     7741 cttatagcta gttccttcca tttcacagca ttaacgtttt ttatattttc gttgtttttc
     7801 tatattagaa aggaaagggt cagtttaaat ttattttttt taatttggtt ttttagtgtc
     7861 atttttagca taagcggatt aaattcgtat attccttcaa ttataccttt ttatgattta
     7921 gtgggtgaaa gtaaggtcaa tatttatctt gaatcaacaa ataatgcaac tggttttcgt
     7981 gttgatttct ttttgttttc actagcccct gtgttaatta tatttattaa cagaacatta
     8041 gtaataaaag atcgctatat taaaatgatt gcattaagtt atttatattt caattctata
     8101 tataatataa tgtcattcat cccattttca gatagagttg ccgcatattc atggtgtctt
     8161 tcccccatta ttctattatg gagtgctctt aagtgtagat catcaattta tatgttaagt
     8221 tctataggga tcgcgttatt aaatcctttg atttttatgt actataattc aatgtttatt
     8281 tttttaaagt gaggatatat gtttaacggt aaaaagattt taatcactgg tggtactggg
     8341 tcatttggta atgcagtgtt acgtcggttt cttgagacag atattcaaga ggttattatt
     8401 ttcagtagag atgaaaaaaa acaagatgag atgagaacgc tatatcgcaa tgataaaatg
     8461 aaatttatta taggtgatat tagagattat acatctgttt tagctgcaac tagtgacgtt
     8521 gactatatat tccatgctgc ggctttaaaa caggtgcctt catgtgaatt ttatccatgg
     8581 gaagcagtta aaacgaatat aattggtaca gaaaatttat taaatgctgc aaaacaaaat
     8641 aaagttaaaa aaacaatttg tttgagcact gataaagctg tgtatccaat taacgcaatg
     8701 ggaatttcga aggctatgat ggaaaaagtg atgacagcaa aggctagaac gttcaacaat
     8761 gaagattcag ttgtttgcgg aactcgttat ggcaatgtta tggcatcacg cggctctgtt
     8821 atccctctgt tcattcagca gattcaaaca aatcaaccat taactataac agaccctgaa
     8881 atgactagat tcatgatgac gttagatgat gctgtagatc tcgtattatt tgcatttaat
     8941 aacgggcaaa acggtgacat ttttgttcag aaagcgcctg ccgcgacaat tgctgtgctt
     9001 gctaaagcat tagttgaatt gatgggggtg ccaaaccatc ctattaaagt agttggttca
     9061 cgtcatgggg aaaagatata tgaaacctta cttagccgtg aagaaatggc aaaagcgatt
     9121 gatcatggag attattatca gattcaacca gataatcgtg atctgaatta tgacaaatat
     9181 ttaagcgaag ggagtaaaaa aattgagctg tttgatgatt acaattccca taatacatat
     9241 agactcaatg ttgatgaaat gaaaaaatta ttgttaaaac tcaatatcat tcaaaatgtg
     9301 ttaaatagtt aaggataatt tataatgcaa aaaatattga tcttaggtgt gagtggtatg
     9361 cttgggcata ccctctttcg ttttttatca tctcagcagg atcttttagt aactggtaca
     9421 gtacgccata ttacgcaaga aataaaatta gtatcatcta agaatatagt cctgatgaga
     9481 gatgtcttta acaaagaaaa attagaaaac attataaatt cgaatgatgt tattataaat
     9541 tgcattggcg caattaagca gaaatatgat aataaaaaca cagattatat tctattaaat
     9601 tcgtactttc cacatcttct taatgaaatt tgtatagaat ctaataaaag actaattcat
     9661 ttctcaacag attgtatttt taacggtgag aaaggaaatt atgatgatgg tagtttatca
     9721 aatgttatag atatgtatgg taaatctaaa tatttaggtg aggtttatgg tgataaaaca
     9781 cttactttaa ggacatcatt aattgggcat gaattaaaat catcgtatag tttgattgat
     9841 tggtttttga actcaaactc aactgttaat ggttatacaa aagctatttt cagtggattg
     9901 ccaaccatag aaattgcgca ttttttatat gagcatgttt tacaatccca gttgcatggt
     9961 atttataatt tatcagcagc accaatttca aaattcgatt tattgacttt agtcaatgaa
    10021 gtatacaaac ttaataaaac catcgttcct aaaagtgact ttgtgattga tcgttctctt
    10081 gactctcata ggttgcgttc attaactaag tatacacctc ctgactggaa tactcttgtt
    10141 gtaaagatgt atttagatta cttaagttta ggagcattgc ttaatgaaag ataacttatc
    10201 tttttctaaa ctgatttctg aagctgagat taactccaga aatcgttctc accttaattt
    10261 gcatgaatct ttttctgatc ctatacagaa aactttgatt tgctgttgtt cgaatacgta
    10321 tattcctcct cactatcata aatttgccca tcaaagtgaa ttgtttatta gattaaaagg
    10381 atgttttagt ctattaattt tttctgttga tggagtgctt acccgtcgta taactataga
    10441 tgaggagaat cctctttatg agataaaacc aggtgttatt catacggtgg tagctttaga
    10501 cagacataac attttacttg aaattaagag gggaccgtat gtcgaatctg aagcgaaaaa
    10561 ttttcctgat tgggtaacat tagaaaatag cgataatcaa cttgatttag agcgattgcg
    10621 aacattgaag gtcggtgata agttatcata acaaggctgt tataaattta ttttaggtga
    10681 tatctatgct tccattaata tctattatta cgccgacata taatagagca tatactctag
    10741 atcggttata taattcttta gtaacacaag actctgaaaa attcgaatgg ataataatcg
    10801 acgatggtag tacagatgaa acacggaaga aatgtttagg ttatataaat gattccattt
    10861 ttaaaataca atattacaaa caaaataata gtggtaaacc cgcagctatc aatttaggag
    10921 taaccaaagc tgttggcgat tatattttta ttgtagatag tgatgatgct ttaacattaa
    10981 atgcaattgg tactatttat gactgtattg taaaatacac agtcgcatct gactttgaat
    11041 tttccggctt aggatttaga aaatcttttt ttgatgggaa aatgctcggc aaacatattg
    11101 agcggaatac gaattcacct atgctgttac atagcacaga ttgtaataat ttatttaaag
    11161 tcgatttagc atattgtttt aaacgagagt tgatgattaa atatcctttc ccaaagttta
    11221 aagatgaaaa atttgttcca gaactgttta tatggaataa aattacagat gttgccaaaa
    11281 taatattttt tcctaatgtt gttgtatatt attgtgagta tctggctgat ggacttacaa
    11341 ataattttaa aaaacaactt cgacataatc caattggctt tgcagtatat tataaagatc
    11401 agttccatag agaacattct atatggggga aaataaaaat ggcaataaga tatatccaat
    11461 gtattatata taaattcata tagtcatgca gggttcatcc attcatttaa ttagttgcta
    11521 tgatttttat atgaaaatat tacatgttgt accacagtta aagcatggtg gtgtcgaaac
    11581 tgctgttttt aattctattg aaaggataag aaagagtgga tatgatttta atgttttgac
    11641 tattgaaaga ggaaactgct ttaacaattc atatgtaaaa gtagaacact gcttaaattc
    11701 ttctgtatac aatccagttt gtttttataa atttattagg tttattcata aagaaaaacc
    11761 tgatttgata gtttattctt tatggaaatc tgcattgtta ggaatttcat tgtgtctttt
    11821 aaaacctttt ctcaaaagta agcctaaaac cgcgttaatt atacataata ctaggtttgc
    11881 acattttgtt gattacataa taacaaaact ttcacttaaa ataaccgact ctatatattt
    11941 tgattcggaa aaatcaaaag cttactttct gaatagagat tatgcaacag gcgaggttat
    12001 tagttttatc gataaaaaga tggtggcaaa aaaatttaat aatatcaatg gtgagattaa
    12061 gtttgtattt attggtaggt tgcactctca aaaaggggtt gatagggcat taaaatatat
    12121 acatcttttt aaaaagcaac atgaagaaat acgatttgat atatatggtc cagacgaagg
    12181 agaattgaat aatattataa ataaggtaaa taaattagga ttgcaggata aagttgtcta
    12241 taaaggtgtt gtcgataata acaaagttaa tgatatatta acccaatatg acttttatct
    12301 tcaattcagc cattttgaag gaatggcaat gtctgttgtt gatgcgatgc aggttggggt
    12361 tattccaatc gttaataatg ttggtgagat accaaattac gtaaaaaaca atattaacgg
    12421 cattatctta gatgataaaa agcttggtga tgaaaactat cttaatttaa atatatcatg
    12481 cttattaaat atcataaaga atgatggtgg atatttttta tataataacg cgataaagac
    12541 atttcaggat aataatactt atgtagatga ctatataaag aaaataataa atgctaacaa
    12601 ctgatatcat gtgttaaaat ggtttatata gctcctacta aataatttgg ctttttcgta
    12661 atggctgtgt agccgcaatc tcgcagtaac cccctgacag gagtaaacaa tgtcaaagca
    12721 acagatcggc gtcgtcggta tggcagtgat gggacgcaac cttgcgctca acatcgaaag
    12781 ccgtggttat accgtctcta ttttcaaccg ttcccgtgaa aagacggaag aagtgattgc
    12841 cgaaaatcca ggcaaaaaac tggttcctta ctatacggtg aaagagtttg ttgaatctct
    12901 ggaaacgcct cgtcgcatcc tgttaatggt gaaagcaggt gcaggcacgg atgctgctat
    12961 tgattccctc aagccatacc tcgataaagg tgacatcatc attgatggtg gtaacacctt
    13021 cttccaggac accattcgtc gtaaccgtga gctttctgca gaaggcttta actttatcgg
    13081 taccggtgtt tccggtggtg aagaaggtgc gctgaaaggt ccttccatta tgcctggcgg
    13141 acagaaagaa gcctataaac tggttgcacc gatcctgacc aaaatcgccg cagtggctga
    13201 agacggggag ccatgcgtta cctatattgg tgccgatggc gcaggtcact atgttaagat
    13261 ggttcacaac ggtattgaat acggtgatat gcagctgatt gctgaagcct attctctgct
    13321 taaaggcggc ctgaatctct ctaacgaaga actggcacag acctttaccg agtggaataa
    13381 cggtgaactg agcagctacc tgatcgacat caccaaagac atcttcacta aaaaagatga
    13441 agacggtaac tacctggttg atgtgatcct ggatgaagcg gctaacaaag gtaccggtaa
    13501 atggaccagc cagagcgcgc tggatctcgg agaaccgctg tcgctgatta ccgagtctgt
    13561 gtttgcacgt tatatctctt ctctgaaaga tcagcgcgtt gccgcatcta aagttctctc
    13621 tggtccgcaa gcgcagccag ctggcgacaa ggctgagttc atcgaaaaag ttcgtcgtgc
    13681 gctgtatctg ggcaaaatcg tttcttacgc tcagggcttc tctcagttgc gtgctgcgtc
    13741 tgaagagtac aactgggatc tgaactacgg cgaaatcgcg aagattttcc gtgctggctg
    13801 catcatccgt gcgcagttcc tgcagaaaat caccgatgca tatgccgaaa atccgcagat
    13861 cgctaacctg ctgctggccc cgtacttcaa gcaaattgcc gatgactacc agcaggcgct
    13921 gcgcgatgtc gtcgcttatg cagtacagaa cggtatcccg gttccgacct tcgccgctgc
    13981 ggttgcctat tacgatagct accgtgccgc tgttctgcct gcgaacttaa tccaggccca
    14041 gcgcgacta
//
