LOCUS       AB811611               16305 bp    DNA     linear   BCT 13-DEC-2014
DEFINITION  Escherichia coli DNA, O-antigen biosynthetic locus, strain: E40.
ACCESSION   AB811611
VERSION     AB811611.1  GI:733166207
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. (2014) In press
   PUBMED   25428893
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16305)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..16305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E40"
                     /db_xref="taxon:562"
                     /note="serogroup:O33"
     gene            <1..904
                     /gene="wcaM"
     CDS             <1..904
                     /gene="wcaM"
                     /codon_start=2
                     /transl_table=11
                     /protein_id="BAQ00789.1"
                     /db_xref="GI:733166208"
                     /translation="YAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILIS
                     DHLIERINCTNGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVEN
                     GKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIK
                     GKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKP
                     QHLFMRNIKVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSV
                     EIDRINHHIVNVEKINFRLPERRE"
     gene            1062..2057
                     /gene="gne"
     CDS             1062..2057
                     /gene="gnu"
                     /note="O033_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAQ00790.1"
                     /db_xref="GI:733166209"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            2300..3193
                     /gene="galF"
     CDS             2300..3193
                     /gene="galF"
                     /note="O033_02"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ00791.1"
                     /db_xref="GI:733166210"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVQY
                     GLRNLKEGAKFRKGIEKLLSE"
     CDS             3493..5004
                     /note="O033_03"
                     /gene="wbeS"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ00792.1"
                     /db_xref="GI:733166211"
                     /translation="MIIAKGDKMIGIILAAGVGSRLRPMTNSKPKCLVTVAGKPILDY
                     QLNSYRLAGIKDIFIIVGYEGDKIKKHCKYIKDLNITIIENNEYEDTNNMYSLFLAKK
                     YAYGESFILNNADLAIDSNIIEKICESPFSDLIAVDVGVFNEESMKVTVNDDNKVSNI
                     SKLIDEKESVGCSIDFYKFSKDSSKIFFDEIERIVLRENNKKDWTEIAMQRLFIDRKM
                     KFDVLDISGCSWVEIDNYADLALADKIFSQKNKKISDYKCYCFDLDGTVYVGREPIKE
                     VIDEINSLKKSGKLIRFISNNSSKCKSEYVNKLKNYGIDVSTEDIKISSDSVIDFLNK
                     EQAKKIYVVGTKSLQKNIIDAGFEICSHEPDFIVLGYDTELTYSKLVTACRLINCGVD
                     YIATHCDVFCPSENGPIPDIGTVVTMLEMTTGRKPYRVFGKPNPDLLNLILNEDRLEK
                     DDLLMIGDRIYTDIQMAENTGIDSVLVLTGDTKREDIEDSSVKPTYILQHFSQ"
     gene            5058..6302
                     /gene="wzx"
     CDS             5058..6302
                     /gene="wzx"
                     /note="O033_04"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ00793.1"
                     /db_xref="GI:733166212"
                     /translation="MLNFTLIKNIFYLFIVQIINYVAPFLVLPYLSRVLSVDNFGLLM
                     MIISASSIALIVTDYGFSLSGPLFVAVNKHNKVVINQYIGTIYLIKSVLISIIWFLFL
                     FIYFISDNEITSHFSNILWLGVIITTQSFQPIWFFQGLEKMKNITFSLIISKSVYVIL
                     IFCLVKTNHVERVFLALVLSNVVTLVISNYLLYRNGYAIGTPCNKLFRDEIKNSFPFF
                     LSRAAVGVYTSASTFIVGSFAGLNQAAVYSSAEKLYQAGQNALSPISQALYPYLARSG
                     DKKTLYKFVVLFFILLCMICILSSYYSNTIVMLFFGNKYNAASQVLNVFLLSLVITFV
                     SFNFGYPAFAAIKRVQIVNYTVVLGGGLQLLMIIILIVSEKITPLNMARSVLFTETLV
                     LISRLGLYFYLILKNDNVSGLK"
     CDS             6299..7438
                     /note="O033_05"
                     /gene="wfxR"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycerophosphotransferase"
                     /protein_id="BAQ00794.1"
                     /db_xref="GI:733166213"
                     /translation="MKKIKFIVKFILGRIVYFLSGFFPRNPNIWVFGSFNGFEDNPKY
                     LYLHVLNKNKEIRSIWIAKDKMELLQARNSGGEAYLFNSIKGFLFTLNAGIYIYSAYI
                     TDISFIASRKAKKVNLWHGIPIKKIEFDITSKPLVNHFSKANFINKMIYAYKHIVPDL
                     LLCPSKYSAEYSFKSAFRISERQCLFARYPRVSALLEMKMNKENKKIILYMPTWRDKK
                     PRFLDTAKIEFEKLNTIMRTYDALFVIKLHPLTIIDRAFEYSNISICNKDSDLNELLF
                     VSDCLVTDYSSVVFDYLHLNKPIIFYNFDMDDYLKGREFYFDYNSIICGEIASDYSSL
                     ESKILAVIKGVDLFERKRKEIYDKFTSETGGNQLIVSSIKKIFEK"
     gene            7450..8652
                     /gene="wzy"
     CDS             7450..8652
                     /gene="wzy"
                     /note="O033_06"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ00795.1"
                     /db_xref="GI:733166214"
                     /translation="MITNYSWHMKNNNIVGMFVLIVSSTIMIYDPWFFGVGRGVVIIG
                     SIFTLISFASGRFFIRKEEVKLLLLLMLFTGYSLLPAIYNYTLDTSVFFMYFKMCIYY
                     ILCFGFVRCFLKKEKLLFVLKGSVVIQCVFILLCLISSDVRNFIFSVHTVEDRFLISE
                     QAYRLYFLSSSSFFQLSLFFGFLFHFMVALYKENKVGLLILILIFVCGAFSGRVFFLF
                     AVISVIFYGINIRYVPLYSVIAFVLIFFAIKFSDNIFIRHALEPLINYINTGEFMTAS
                     SDKLVEKMLFIPDFSALVVGDGVYYNDDGSYYMHTDSGIIRQFLYGGGGYFISCMALT
                     WYLLTKVSKNWLEKNKKFTLSTMIIFLIGNIKADVLMYPGIMINLIFILIFASGDNND
                     KSVYRILK"
     CDS             8621..9364
                     /note="O033_07"
                     /gene="wclP"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00796.1"
                     /db_xref="GI:733166215"
                     /translation="MIKVFIVSLNDSPRRTSISQLCEKYGVEFEFVDAVNGRKLDDKY
                     INKINSYEWTKLKYKRKLGPAEIGCTLSHLSIYKKNINSWFIVLEDDVDFDERFVKLI
                     NTDITGLRTDALYLLGGQEGLESFKNVIFSRFGGIKVNTDIVFLKTICSERFLFRTCC
                     YMIHHSLAKRIVDFNQNKLTLADDWFNLSKFNIINNIYYIDLVAHPSDLSTSLIHSDR
                     LKLIKTRQKIYRTFMKRIVLKFRSILRFF"
     CDS             9367..10260
                     /note="O033_08"
                     /gene="wccM"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00797.1"
                     /db_xref="GI:733166216"
                     /translation="MFSIIIPSFNREKQLRNALAELNKQLYKEFEVIVVDDASDSEYD
                     LSLDLYHFELKYFRSNKNLGPSGARNIGVSLARYDWIIFLDDDDYFMPEKLMILRETI
                     LNNDIDFIYHRARIFMINEKISYISSQKDIDKIPGEVYQHMLSGNFIGGPPNFAIKKS
                     LFNKLHGFSDNVRAIEDYEFLLRLVRDVSKKRIIFVDNVLTGCNYITKSTSVSKNINN
                     LKQACTYISEEYIRDNREFNLFKINTSLMTAHALLMSLNRKSAFFYFKAGIVMFNIKY
                     IVSGIISFISPVLMIKLRGKV"
     CDS             10269..11114
                     /note="O033_09"
                     /gene="wbwC"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00798.1"
                     /db_xref="GI:733166217"
                     /translation="MSDGKENRYQKFSVLMSVYYKEDPVFFYDAVNSVFENTIPPDDV
                     VIVVDGPIPEGITDVILALREKYELNIVYLSENVGLGRALNIGINYCKYNIIMRMDTD
                     DYCINTRFQEQLDFFSTHSDVVLLGGDIAEFDTELSNFIGVRHVAYTCQSIRDMAKKR
                     NPFNHMTVAFKKDIILSVGGYKHHPFMEDYNLWLRVIAKGHKVANLPKVMVNVRAGES
                     MLSRRKGIRYIYSEYVLYKLKLSLGIDSLVHGFFIFLMRAFIRVIPIGMISILYSSIR
                     KNMNT"
     gene            11237..12643
                     /gene="gnd"
     CDS             11237..12643
                     /gene="gnd"
                     /note="O033_10"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ00799.1"
                     /db_xref="GI:733166218"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDIGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELIAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDGDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            12891..14057
                     /gene="ugd"
     CDS             12891..14057
                     /gene="ugd"
                     /note="O033_11"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ00800.1"
                     /db_xref="GI:733166219"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDNIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            14147..15181
                     /gene="wzz"
     CDS             14147..15181
                     /gene="wzz"
                     /note="O033_12"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ00801.1"
                     /db_xref="GI:733166220"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGS
                     AAPKVSEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGKTP
                     EGAQKQLAKYIQQVDDQVNEELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(15367..15978)
                     /gene="hisI"
     CDS             complement(15367..15978)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ00802.1"
                     /db_xref="GI:733166221"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     4856 a   2831 c   3468 g   5150 t
ORIGIN
        1 ttacgccatc ttacgccagg gatttcataa ccagattatc ggtgccaaca tcaccaattg
       61 taagttcagc gacttacaag gcgacgccat tgaatggaac gtggcaatta acgaccgcga
      121 tattttgatc tccgaccatc tcatcgagcg catcaactgt accaacggca aaatcaactg
      181 gggcatcggc ataggccttg cgggaagtac ctacgataat aattatccgg aaaaccaggc
      241 agtgaaaaac tttgtcgtgg cgaatatcac gggatcggat tgtcgacagt tgatccatgt
      301 tgaaaatggt aaacattttg ttattcgtaa tatcaaagcc cgtaatatca caccggattt
      361 cagtaagaaa gcaggtattg ataacgccac ggtcgctatt tacggttgtg ataatttcgt
      421 gattgataat attgaaatga ttaatagtgc cgggatgtta atcggctatg gggtaattaa
      481 aggcaaatat ctctcgatac cgcagaattt ccgggtgaat aatattcaac tggataacac
      541 ccaccttgct tataaattgc gcggcatcca aatctccgcc gggaatgctg tctcctttgt
      601 ggcgctgact aacattgaga tgaagcgtgc gtcattggag ttacacaaca agccgcaaca
      661 tctttttatg cgtaatatca aggtgatgca ggaatcctca gttggaccag cattgagcat
      721 gaactttgac atgcgcaaag acgttcgagg cgtgtttatg gcgaaaaaag aaacactgct
      781 gtctcttgca aatgttcatg cggtgaatga aaaagggcaa agctccgtcg agatcgacag
      841 gattaatcac catattgtta atgtggaaaa gattaacttt agattgccgg aacggaggga
      901 gtagatttgc gaccattcat aaacttactg gatataatgt tctaaaagat tataatcctc
      961 acactaactg ttaaagctgg ggctgggcag cattgactac tcagggaagg tactcatctt
     1021 tatcttgtgt ttcttacgta acaaccagac taaagtgatt tatgaacgat aacgttttgc
     1081 tcataggagc ttccggattc gtaggaaccc gactacttga aacggcaatt gctgacttta
     1141 atatcaagaa cctggacaaa cagcagagcc acttttatcc agaaatcaca cagattggcg
     1201 atgttcgtga tcaacaggca ctcgaccagg cgttagccgg ttttgacact gttgtgctac
     1261 tggcagcgga acaccgcgat gacgtcagcc ctacttctct ctattatgat gtcaacgttc
     1321 agggtacccg caatgtgctg gcggccatgg aaaaaaatgg cgttaaaaat atcatcttta
     1381 ccagttccgt tgctgtttat ggtttgaaca aacacaaccc tgacgaaaac catccacacg
     1441 atcctttcaa ccactacggc aaaagtaagt ggcaggcaga ggaagtgctg cgtgaatggt
     1501 ataacaaagc accaacagaa cgttcattaa ccatcatccg tcctaccgtt atcttcggtg
     1561 aacggaaccg cggtaacgtc tataacttgc tgaaacagat cgctggcggc aagtttatga
     1621 tggtgggcgc agggactaac tataagtcca tggcttatgt tggaaacatt gttgagttta
     1681 tcaagtacaa actgaagaat gttgccgcag gttatgaggt ttataactac gttgataagc
     1741 cagacctgaa catgaaccag ttggttgctg aagttgaaca aagcctgaac aaaaagatcc
     1801 cttctatgca cttgccttac ccactaggaa tgctgggtgg atattgcttt gatatcctga
     1861 gcaaaattac gggcaaaaaa tacgctgtca gctcagtgcg cgtgaaaaaa ttctgcgcaa
     1921 caacacagtt tgacgcaacg aaagtgcatt cttcaggttt tgtggcaccg tatacgctgt
     1981 cgcaaggtct ggatcgaact ctgcagtatg aattcgttca tgccaaaaaa gacgacataa
     2041 cgtttgtttc tgagtaatgc agacgagtca gagaaaaaag gaccaggttg gactacgatt
     2101 attcggaaag aggtactgat taaattatca gtatgcggaa ttccgagtaa tccgagtaac
     2161 ccagatgctg atggtgtgta gtgttattct aaggtttaag caaagttatg cggaactaag
     2221 ctttggtata tagccataac gtttggctaa cctatggcaa taatttacgc actatcagcc
     2281 acttagaggt aggaaaagaa tgacgaattt aaaagcagtt ataccggtag caggtcttgg
     2341 gatgcatatg ttgcctgcca ctaaggcgat tcccaaagag atgctaccga tcgtcgacaa
     2401 gccaatgatt cagtacatcg ttgacgagat tgtggctgca gggatcaaag aaatcctcct
     2461 ggtaactcac gcgtccaaga acgcggtcga aaaccacttc gacacctctt atgaattaga
     2521 atctctcctt gaacagcgcg tgaagcgtca actactggcg gaagtacagt ccatttgccc
     2581 gccgggcgtg accattatga acgtgcgtca gggcgaacct ttaggtttgg gccactccat
     2641 tttatgtgca cgaccagcta ttggtgacaa cccatttgtc gtggtactgc cggacgttgt
     2701 tatcgacgac gccagcgctg acccgctgcg ctacaacctt gctgccatga ttgcgcgctt
     2761 caacgaaacg ggccgcagcc aggtgctggc aaaacgtatg ccgggcgatc tctctgaata
     2821 ctccgtcatt cagaccaaag aaccgctgga tcgtgaaggt aaagtcagcc gcattgttga
     2881 atttatcgaa aaaccggatc aaccgcagac gctggactca gacattatgg ccgttggtcg
     2941 ctatgtgctt tctgccgata tttggccgga actggaacgc actcagccag gtgcatgggg
     3001 gcgtattcag ctgactgatg ccattgctga actggcgaaa aaacagtcag ttgatgcaat
     3061 gttaatgacg ggggacagct acgattgcgg taaaaaaatg ggctacatgc aggcatttgt
     3121 gcagtacgga ctacgcaacc tgaaagaagg ggcaaagttc cgtaaaggga ttgagaagct
     3181 gttaagcgaa taaaatggtt aagaaggaaa ttataacggc aatgaagatt tgttcgaaag
     3241 ttgtgtattg aaaatattct tgccgctgat tttttatagc taatcatatt agcaagaggt
     3301 ttataagaag atgttagtca agtttttccg ggatttacct tgttttcaga gtcgataggg
     3361 aagacaatta gcgtttagat ttttctggat tggttagaga gtgtaatgct cgttacattg
     3421 tagatatgta tacagtgctc tggtagctgt taagccaagg gcggtagcta tttgaaatca
     3481 tactaaaaca atatgattat tgcaaaaggt gataaaatga tcggtattat tctggctgca
     3541 ggtgttgggt ctcgtttgcg gcccatgact aatagtaaac ctaagtgtct tgtgactgtt
     3601 gctggcaagc ctatcttgga ttatcagtta aactcctatc gcctggctgg tattaaagat
     3661 atattcatta tcgttggcta tgaaggtgat aaaataaaaa aacactgtaa atatattaaa
     3721 gatctcaata ttacaataat agaaaataat gagtatgaag atacaaataa tatgtattca
     3781 ctctttttag caaagaaata tgcatatgga gaatctttta tattaaacaa tgcagatcta
     3841 gctattgatt caaatatcat agagaagatt tgtgagagtc ccttcagtga tcttattgct
     3901 gttgatgtag gtgtttttaa tgaagagtct atgaaggtta ctgtaaatga tgataataaa
     3961 gtaagtaata tatctaaatt aatcgatgag aaagagtctg ttggttgttc tatagatttc
     4021 tataaattct ctaaggattc aagtaagatc tttttcgatg aaatagaacg aattgtgtta
     4081 agggagaata ataagaaaga ttggactgag attgcaatgc aacgtctatt tatagataga
     4141 aagatgaaat ttgatgtttt agatattagt ggatgttctt gggttgaaat tgataactat
     4201 gctgatttag ctcttgctga taaaatattc tcccaaaaaa ataaaaaaat atcggattat
     4261 aaatgttatt gctttgattt agatggtaca gtctatgtcg gtagagagcc gattaaagaa
     4321 gttatagatg aaataaatag tttaaagaaa agtggtaagt tgattcgttt tatatcaaat
     4381 aactcatcaa agtgcaaaag tgaatatgta aataagctga aaaattacgg tatagatgta
     4441 tctactgaag atattaaaat atcatctgat agtgttattg actttttaaa taaagaacag
     4501 gctaagaaaa tatacgtagt tggaacaaaa agtttacaaa aaaatataat tgatgctggt
     4561 tttgagattt gttctcatga acctgatttt atcgtgttag gatatgatac tgagctgact
     4621 tattcaaaat tggtaaccgc ttgtaggtta attaattgcg gggttgatta tattgcaacg
     4681 cattgtgatg ttttttgtcc ctcggaaaac ggtcctattc ctgatattgg cactgtagta
     4741 acaatgctag aaatgacaac tgggcgtaag ccgtaccgcg tatttggtaa accaaatcca
     4801 gatcttttga atttgattct taatgaagat cggctggaaa aagatgattt gttaatgatt
     4861 ggtgaccgta tatatacaga tattcaaatg gctgaaaata caggcattga ttctgttctt
     4921 gtattaacag gtgacactaa acgagaggat attgaggatt catctgtaaa accgacctat
     4981 atacttcaac acttctctca gtaattttca acaaataata acctgtgttt taagcaggtt
     5041 attatttgga gccatctatg ttaaatttta cccttattaa gaatatattt tatttattta
     5101 ttgtacaaat aataaattat gttgctcctt ttttagtttt gccttattta agcagggttc
     5161 tgtccgttga taactttggc ttgctaatga tgataatatc tgcaagttct atcgcattaa
     5221 ttgttactga ttatggattc agcttatcag gacctctgtt tgtggctgta aataagcata
     5281 ataaagtagt cataaatcaa tatattggaa caatatattt aataaaatct gtgttaatta
     5341 gcattatatg gtttttattt ctctttatat attttataag tgataatgaa attacatcac
     5401 atttttcaaa tatattatgg ctaggtgtta taataacaac gcagtccttt cagcctattt
     5461 ggttttttca agggctagag aaaatgaaaa atataacgtt ttctctaatt atatccaaat
     5521 cggtttatgt aatattgatt ttctgccttg taaagacaaa tcacgtggag cgtgttttct
     5581 tggcattagt tctaagtaat gttgtaactt tagtaataag taattatctt ttatatcgta
     5641 atggttatgc gataggcaca ccttgtaata aattatttag agacgaaata aaaaatagtt
     5701 ttccattttt tttatcaagg gctgctgtag gtgtttatac tagtgcaagt acctttattg
     5761 ttggcagttt tgcagggtta aatcaggcgg ctgtttattc gagcgctgaa aaattatatc
     5821 aagcagggca aaatgcttta tcgccaatat ctcaagcatt atatccatat ttagcaaggt
     5881 ctggcgataa gaaaacttta tataaatttg ttgttctgtt ttttattttg ctctgtatga
     5941 tatgcatatt gagttcgtat tattctaata ccatagtgat gttatttttt ggtaataaat
     6001 ataatgcggc atctcaggtt ttaaatgttt ttctcttaag tcttgttatt acttttgtta
     6061 gttttaactt tggatatcct gcttttgctg caattaaacg agtgcagatt gtcaattata
     6121 cagttgttct ggggggcgga ttgcagttat tgatgattat cattttaata gttagtgaaa
     6181 agatcacccc tctgaatatg gctcgaagtg tgttatttac tgaaacacta gtgttgattt
     6241 ctagattagg tttatatttc tatcttattc tcaagaatga taatgtatca ggtttgaaat
     6301 gaaaaaaata aaatttatcg ttaaatttat tttgggacga attgtttact ttttgtctgg
     6361 tttttttcct agaaatccaa atatatgggt ttttggtagc tttaatgggt ttgaggataa
     6421 tccaaaatat ttatatctac atgttcttaa taagaacaaa gaaataaggt ctatatggat
     6481 agcaaaagat aaaatggaat tgctgcaggc taggaatagt ggaggtgagg cttatctttt
     6541 taatagcata aaaggatttt tgtttacctt aaatgcaggt atatatatat attcagcata
     6601 tattacggat atatctttta ttgcatccag aaaagctaaa aaagttaatt tatggcatgg
     6661 gattcctatc aagaaaatag agtttgacat aacatctaaa ccgttggtca accatttctc
     6721 taaagccaat tttataaata agatgatata tgcatataag catatagttc ctgatcttct
     6781 tctttgtcca agtaaatact cggctgagta ttcatttaaa tcggcattcc gtatttctga
     6841 gagacagtgt ttatttgcaa gatatccaag agtatccgca ttacttgaaa tgaagatgaa
     6901 taaagagaat aaaaaaataa ttctttatat gccaacttgg cgtgataaaa aacctaggtt
     6961 tctagatacg gcaaaaattg aatttgaaaa gctgaatact atcatgagaa catatgatgc
     7021 attatttgtt attaaactac atccacttac tatcatagat agggcttttg aatattctaa
     7081 cattagtatt tgcaataaag actctgattt aaatgaactt ttatttgttt ccgattgtct
     7141 ggtaacagat tattcgtctg ttgtttttga ttatctacat ttgaataaac caataatatt
     7201 ttataatttt gatatggatg actatctgaa agggcgagag ttttatttcg attataactc
     7261 aataatttgt ggcgaaattg cttctgatta ttctagttta gagtcaaaaa ttttagctgt
     7321 gattaaagga gtagatcttt tcgaaaggaa aaggaaggaa atatacgata aatttacttc
     7381 tgagactgga ggaaatcaac ttatagttag ttctattaag aaaatatttg aaaaataaag
     7441 agagttctta tgataactaa ttattcgtgg catatgaaaa ataataatat tgtaggaatg
     7501 tttgttttaa ttgtttcttc tacaatcatg atatatgacc catggttttt tggtgtgggg
     7561 cgtggtgttg ttattattgg tagcattttt actttaattt cttttgcatc aggacgattt
     7621 tttataagga aagaggaagt taaacttctg ctgcttttaa tgctttttac aggatattcc
     7681 ttattgcctg caatatataa ttatacctta gatactagtg tattttttat gtattttaaa
     7741 atgtgcatat attatatact ttgttttggg tttgttaggt gttttttgaa aaaagaaaaa
     7801 ctattattcg ttttgaaagg aagtgttgtt atacaatgtg tatttatctt actgtgttta
     7861 atttcctctg atgtccgaaa ctttatattt tcagttcaca ctgttgaaga tagatttctt
     7921 atttcagagc aagcatatag actttatttc cttagctcat catctttttt tcaattaagt
     7981 ctcttttttg gattcttatt ccatttcatg gtcgctttat ataaagaaaa taaagttggt
     8041 ttacttattt tgatattgat ttttgtatgt ggggcttttt ctggtcgagt atttttccta
     8101 tttgcagtta tttctgttat tttttatggt attaacataa gatatgtgcc gttatatagt
     8161 gtcatagctt ttgttttgat attttttgct atcaagtttt ctgataatat ttttatcaga
     8221 catgcactgg aaccattgat taattacatt aatacaggag agttcatgac tgcatcaagt
     8281 gataagttgg tggaaaaaat gttgtttatt cctgattttt ctgcattggt cgtaggtgat
     8341 ggtgtatatt ataatgatga tggttcctat tatatgcata ctgattcagg tatcataagg
     8401 caatttttgt atggtggagg ggggtatttt atatcttgta tggcattaac ttggtattta
     8461 ctgactaaag tctcgaaaaa ctggcttgaa aaaaataaaa aatttactct atcaacaatg
     8521 attatttttt tgatcggaaa tatcaaagcc gatgttctaa tgtatccagg cattatgata
     8581 aacttaattt ttattttaat tttcgcgtca ggagataata atgataaaag tgtttatcgt
     8641 atccttaaat gattcaccga gaagaacatc aatctcacaa ttgtgtgaaa aatacggtgt
     8701 agaatttgag tttgttgatg cagttaatgg cagaaaatta gatgataaat atatcaataa
     8761 aattaattct tatgagtgga caaagttaaa atataaaaga aagttaggtc cagcagagat
     8821 tggttgtaca ttaagtcatc tcagtatata caaaaaaaat attaatagtt ggttcatcgt
     8881 tttggaggat gatgtcgatt ttgatgaacg atttgtaaaa ttaatcaata ctgatataac
     8941 gggtttaagg actgatgctc tctatcttct tggtggacaa gagggacttg agtcatttaa
     9001 aaatgttatt ttttcaagat ttggtggtat taaggtgaat acagatatag tctttcttaa
     9061 gactatctgt agcgaacgtt ttttatttcg aacatgttgc tatatgattc atcattcgtt
     9121 agctaagaga atagtagact tcaaccaaaa taaacttact ttagctgatg attggtttaa
     9181 tttatcaaag tttaatatca taaacaatat atattatatt gatcttgttg cacatcctag
     9241 tgatctgagt acttctctaa ttcattcaga taggctaaaa ttaattaaaa caaggcaaaa
     9301 aatatataga acattcatga aaagaatagt attaaaattc aggtctatat tgaggttttt
     9361 ctgatcatgt tttcaattat aatacctagc tttaatcgag agaaacaact tcgtaatgca
     9421 ttggccgaac taaataagca actctacaaa gagtttgagg ttattgttgt tgatgatgct
     9481 tcggacagtg aatatgattt gagcttggat ctttatcatt ttgaactgaa atatttcaga
     9541 agcaataaaa atttaggacc atcaggtgct agaaatattg gagttagttt agctcgttat
     9601 gattggatca tttttttgga cgatgatgat tatttcatgc cggaaaaact gatgatcttg
     9661 agggagacta tattaaataa tgatattgac tttatctatc atagagctcg tatatttatg
     9721 atcaatgaaa aaattagtta tatttctagt caaaaggata tagataaaat tccaggtgag
     9781 gtatatcaac atatgctttc tggtaatttt attgggggcc ctccaaattt tgcaataaaa
     9841 aaaagcctgt ttaacaaact tcatggcttt tctgataatg tacgagcaat tgaagattac
     9901 gagtttctac taaggttagt ccgtgacgtt agtaaaaaaa gaattatttt tgtagataat
     9961 gtgcttactg ggtgtaatta tataacaaaa tcaaccagcg tctctaaaaa tataaacaat
    10021 ttaaaacaag catgtacata tatatcagag gaatatattc gagacaaccg cgaatttaat
    10081 ctttttaaaa taaatacctc gttgatgact gcacatgctt tgttaatgag tttaaatcgt
    10141 aaatctgctt tcttttattt taaagcaggg attgttatgt ttaatataaa atatattgta
    10201 tctgggatta tttcttttat atctccagtt ttgatgatta agcttagggg caaggtttaa
    10261 taataattat gagtgatggg aaagaaaata gatatcaaaa gtttagtgtt ttgatgtccg
    10321 tgtactataa agaagatcct gttttttttt atgatgctgt taatagtgtg tttgagaata
    10381 cgatccctcc agatgatgtg gtcattgtcg ttgatggacc tatcccggaa ggtattacgg
    10441 atgtaatatt ggcgctaaga gaaaaatatg aacttaacat tgtttattta agtgaaaacg
    10501 ttggtttagg gagagctcta aatataggta ttaattattg caaatataat attataatga
    10561 gaatggatac tgatgattat tgtattaata caaggtttca ggaacaatta gatttttttt
    10621 ccactcattc tgatgtggtc cttcttggtg gggatattgc agaattcgat actgaactgt
    10681 ctaattttat tggtgttaga cacgtggcgt atacttgtca aagtataagg gatatggcaa
    10741 aaaagagaaa tccatttaat catatgacag tagccttcaa aaaagatata atattatcag
    10801 ttggtggata taagcatcat ccatttatgg aggattacaa tctttggtta agagttattg
    10861 ctaagggaca taaagtagct aatcttccta aagttatggt taatgtgaga gctggtgaaa
    10921 gtatgctttc tcgacgaaaa ggaattcgct atatatatag tgagtatgta ttatataaac
    10981 tcaagttgag cttgggtatt gatagtttag ttcatggttt ttttatattt ttgatgcgtg
    11041 catttattcg tgttattcca attggtatga taagtatctt atattcaagt ataagaaaaa
    11101 acatgaatac ataagtggat gttaaagatg atattacacg ttttgtatat ttaaatcgtg
    11161 atttgctata atagaaatgc tcacataatc tatcagatct attaaactaa gtaagccccc
    11221 ctgacaggag taaacaatgt caaagcaaca gatcggcgtc gtcggtatgg cagtgatggg
    11281 gcgcaacctt gcgctcaaca tcgaaagccg tggttatacc gtctctattt tcaaccgttc
    11341 ccgtgaaaag acggaagaag tgattgccga aaatccgggc aagaaactgg ttccttacta
    11401 tacggtgaaa gagtttgttg aatctctgga aacgcctcgt cgcatcctgt taatggtgaa
    11461 agcaggtgca ggcacggatg ctgctattga ttccctcaag ccgtacctcg atataggtga
    11521 catcatcatt gatggtggta acaccttctt ccaggacacc attcgtcgta accgtgagct
    11581 ttctgctgaa ggctttaact tcatcggtac cggtgtttct ggaggtgaag agggcgcgct
    11641 gaaagggcct tccatcatgc ctggtgggca gaaagaagcc tatgaactga ttgctccgat
    11701 cctgaccaaa atcgccgccg ttgctgaaga tggcgaaccg tgcgttacct atattggtgc
    11761 cgatggcgcg ggtcactatg tgaagatggt tcacaacggt attgaatacg gtgatatgca
    11821 actgattgct gaagcctatt ctctcctgaa aggcggcctg aatctctcta acgaagaact
    11881 ggcacagacc tttaccgagt ggaataacgg tgaactgagt agctacctga tcgacatcac
    11941 caaagacatc tttaccaaaa aagatggaga cggtaactac ctggttgatg tgatcctgga
    12001 tgaagcggct aacaaaggta ccggtaaatg gaccagccag agcgcgctgg atctcggaga
    12061 accgctgtcg ctgattaccg agtctgtgtt tgcacgttat atctcttctc tgaaagagca
    12121 gcgtgttgcc gcatctaaag ttctctctgg cccgcaagcg cagccagctg gcgacaaagg
    12181 tgagttcatc gaaaaagttc gtcgtgctct gtatctgggc aaaatcgttt cttacgctca
    12241 gggcttctct cagctgcgtg cggcgtctga agagtacaac tgggatctga actacggcga
    12301 aatcgcgaag attttccgtg ctggctgcat tatccgtgcg cagttcctgc agaaaatcac
    12361 cgatgcttat gccgaaaatc cgcagatcgc taacctgctg ctggctccgt acttcaagca
    12421 aattgccgat gactaccagc aggcgctgcg tgatgtcgtt gcttatgcag tacagaacgg
    12481 tatcccggtt ccgaccttcg ctgctgcggt tgcctattat gacagctacc gtgccgctgt
    12541 tctgcctgca aacctgatcc aggcacagcg tgactatttc ggtgcgcata cttataagcg
    12601 cattgataaa gaaggtgtgt tccatactga atggctggat taatctgatt taaccaacaa
    12661 taaattgagg cccggcgtat attgtgtcgg gctttttttt gccaattatc ttatagacta
    12721 aatttcactg cttaatatta acttaataaa tatcagctat tcttataaag aaattctgaa
    12781 ttgtttttcg ttgcgttgac cagctatcta cgtagcgtta aaacttttaa ctcttatcag
    12841 gatgctaaaa acatcatgat tcacagttaa gttaattctg agagcatgaa atgaaaatca
    12901 ccatttccgg taccggttat gtaggcttgt caaacgggct tctaatcgca caaaatcatg
    12961 aggttgtggc attagatatt ttaccgtcac gcgttgctat gctgaatgat cggatatctc
    13021 ctattgttga taaggaaatt cagcagtttt tgcaatcaga taacatacac tttaatgcca
    13081 cattagataa aaatgaagcc taccgtgatg ctgattatgt catcatcgcc actccaaccg
    13141 actatgatcc taaaactaat tatttcaata cgtctagtgt agaatcagta attaaagacg
    13201 tagtagagat aaatccttat gcggttatgg tcatcaaatc cacggttccc gttggtttta
    13261 ccgcagcgat gcataagaaa tatcgtactg aaaatattat tttctccccc gaattcctcc
    13321 gcgaaggtaa agccctttac gataaccttc atccgtcacg tattgtcatc ggtgagcgtt
    13381 cggaacgcgc agaacgtttt gcggcgttat tacaggaagg cgcgattaag caaaatatcc
    13441 cgaccctgtt caccgattca accgaagcag aagcgattaa acttttcgct aatacctatc
    13501 tggcgatgcg cgtagcatac tttaatgaac tggatagcta tgcagaaagt ttgggtctga
    13561 atacgcgtca gattatcgaa ggtgtttgtc tcgatccgcg tattggcaac cattacaaca
    13621 atccgtcgtt tggttatggt ggttattgtc tgccgaaaga taccaagcag ttactggcga
    13681 actaccagtc tgtgccgaat aacctgattt cggcaattgt cgatgctaac cgcacgcgta
    13741 aagactttat tgccgatgcc attttgtcac gcaaaccgca agtggtgggt atttatcgtc
    13801 tgattatgaa gagcgggtca gataacttcc gtgcgtcttc cattcagggg attatgaaac
    13861 gtatcaaggc gaaaggcgtt gaagtgatca tatacgagcc ggtgatgaaa gaagattcat
    13921 tcttcaactc tcgtctggaa cgtgatctcg ccactttcaa acaacaagcc gacgtcatta
    13981 tctccaaccg tatggcagaa gagcttaagg atgtggcaga caaagtctac acccgcgatc
    14041 tctttggcag cgactaacat cctgttatca gggcgatttt cgccctgatt ctctcatgtt
    14101 ccctttgtaa taattcattg tttttatcat ttatcctata gcattcatgg cgattatcgc
    14161 taaactatgg cgacttaaaa ttatttcgtc cgttagggta atgatgagag tagaaaataa
    14221 taatgtttct gggcaaaacc atgatccgga acagattgat ttgattgatt tactggtcca
    14281 actatggcgc ggcaagatga ctatcatcat ttctgtcatt gtggctattg ccctggctat
    14341 agggtatctg gccgttgcca aagaaaagtg gacttcaaca gctatcgtta ctcaacctga
    14401 tgtgggacaa atcgctggtt ataacaatgc cataaatgtg atttatggtt cggcagcgcc
    14461 gaaagtgtct gagatccagt cgatgttaat tggtcgtttc agcacgacat tttctgcatt
    14521 agcagaaacg ctggataatc aggaagaacc tgaaaaactg accattgagc ctaccgttaa
    14581 aaatcagtca ttacctttag cggtttctta tgtcggtaaa acacctgaag gtgcgcagaa
    14641 gcagctggcg aagtatatcc agcaagttga cgaccaggta aatgaagaac tggaacaaga
    14701 cctgaaagac aacatcgcgc tgcaaatgaa aaacttgcag gattctctga agacgcagga
    14761 agtggtcgct caagaacaga aagatctgcg tatccgtcag attcaggaag cgttgcagta
    14821 tgcgaatcag gcgcaggtga caaagccgca gattcaacag acacaggacg taacccagga
    14881 tactatgttc cttttgggga gcgaagcgct ggagtcgatg attaagcacg aagcgacccg
    14941 tccgttggtg ttctcatcaa actactatca gactcgtcaa aacctgctgg atatcgacaa
    15001 tcttgacgtt gataaacttg atattcatgc ttaccgctat gtaatgaaac cgacgttacc
    15061 tattcgtcgc gatagcccga agaaagcaat taccttgatt ctggctgtgc tgctgggcgg
    15121 catggttggc gcggggattg tgcttgggcg taacgcgctg cgtaattaca acgcgaaata
    15181 atattattgt gcatttaaga gaaacgggca gggtggtgac accatgcccg ttttttttgc
    15241 cggatgcgat gctggcgcat cttatccggc ctacgtgtgt tgagataatg tgtaggcacg
    15301 ataagcttgc gcatcgggca atggctccgg gtgtgacaac aacatcacac ctgctcagca
    15361 cgccactcac tgatgccgtt tacgcaggtt ctcaattacc gttgttaaat ccagatcctg
    15421 atcctgcaac aacaccagca ggtgatacat caagtcagat gcctcgttgg tcagctcaaa
    15481 gcggtcatgt accgttgctg ccagtgcggt ttcaacgcct tcttcaccca ctttctgcgc
    15541 aatgcgtttg gtgccgctgg catacagttt ggcggtgtag gaggtttccg ggtcggcgga
    15601 tttacgttcc gcgagcagtt gttccagttg atacaggaac agccactggt gagcggtatc
    15661 gccgaagcag ctgctggtgc ctttgtggca ggttggcccg atgggattcg ccagcaccag
    15721 taacgtgtcg ttgtcgcagt ccggcgcaat atttactacg ttgaggaaat tacccgacgt
    15781 ttcgcctttg gtccacagtc gctgtttagt gcgcgagaag aaggtgactt tgccgctttc
    15841 gatggttttg tccagagctt ccgggttcat atagcccagc attagcactt cgccagatac
    15901 cgcgtgttgc acaatcaccg gcatcagtcc gtcggttttt tcccagtcca gttcgcgacg
    15961 ttgttgttct gttaacatat cctgatctcc acgccctgtg ttgccaggta cgcttttaat
    16021 tcaccaatat tgattatttg tttgtggaat acggaagcag ccagcgcacc gtcaacgtcg
    16081 gcatcgcgga aggcttccag gaagtgttcc atggtgcccg cgccaccgga ggcaatcagc
    16141 gggacgtggc aaacttcacg cacttttttc agctgttcga ggtcgtaacc gttacgcacg
    16201 ccgtcctgat tcatcatgtt gaggacgatt tctccggcac cgcgtttttg cacttcctgc
    16261 acccagtcga gagtttccca ctgagtaacg cgggtgcggc tttca
//
