LOCUS       AB811615               15239 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli DNA, O-antigen biosynthetic locus, strain:
            F11621-41.
ACCESSION   AB811615
VERSION     AB811615.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 15239)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..15239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F11621-41"
                     /db_xref="taxon:562"
                     /note="serogroup:O38"
     gene            <1..867
                     /gene="wcaM"
     CDS             <1..867
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="BAQ00854.1"
                     /translation="IGANITNCKFSDLQGDAIEWNVAINDSDILISDHIIERINCTNG
                     KINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKAR
                     NITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFRV
                     NNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKVMQ
                     ESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIVNV
                     EKINFRLPERRE"
     gene            1025..2020
                     /gene="gne"
     CDS             1025..2020
                     /gene="gnu"
                     /note="O038_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAQ00855.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            2263..3156
                     /gene="galF"
     CDS             2263..3156
                     /gene="galF"
                     /note="O038_02"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ00856.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAVIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGESYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     CDS             3488..4303
                     /note="O038_03"
                     /gene="wffK"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00857.1"
                     /translation="MKIAIGISTLNNGINQVWEKIKNIPDEFLIIISHQVTDKQKQYG
                     DICKKNNVIIITTYCKGLSKSRNILLATAFQKSVDYMIISDDDVAYLVNGLYELKDRI
                     IEDRGKYHYQIQSCTQDGRLRKKYPLMRKRLNRLSAFNISSIEMCLNVNQIQECNVWF
                     DECFGLGARYKAGEEPIFVTDLMKTKNNIIFIPVTITVHPIESSGKKIYNETDALSDR
                     SAIFVRCGGRYLGLLYIFIFWVKKFLFKKKCGRIKKIAALFILLKGYSRYEYL"
     gene            4290..5516
                     /gene="wzy"
     CDS             4290..5516
                     /gene="wzy"
                     /note="O038_04"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ00858.1"
                     /translation="MNIYKNSNENSLSNNNCIVYFIPLLLLATFFFPLVVLIFIGALS
                     PLLHPVLRNFYFYALLVFIIIFFSTLKPFGDIAEYLHVYHELNYNLIDVFGYSRFGDG
                     LEFMFLAIMKFIGYISGGNDEVFLLSTYFLIVFFLSKILKDVDKKYKLFLLSLFFFNL
                     GFIEVTSYFLRQVLSVVVFLYAINERSFKKYIFFLLSVFFHMSAVVNVFIYAVYMIFG
                     SKKYSYAKIVFSILIGLLVVFFVVYNTPIYSVLLSKFTSVSGNDKFTRLPLNYIIITV
                     VNICFIIMIGKRSKCDDFNKILFCKECFLFLILLPFPALSNRLGMIIFGFYPYFILPY
                     LKAFERKGKSKYALLICLYVVNLVPFLYLMYNVSLGNNMFTFLNNHPFTEGVYGMIDY
                     ILEAIDKGVNYINEGN"
     gene            5520..6827
                     /gene="wzx"
     CDS             5520..6827
                     /gene="wzx"
                     /note="O038_05"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ00859.1"
                     /translation="MKKYLSNLLWLLSDRVFMLVFQLSLFASIKRIYGLDILGSWATI
                     MNISQILLSLFLFGIDIVVVKRIVENPSSTGTEIGCALFLQFLGLLLYASAFITIVIY
                     FYYDIPFAFIFVAILIVANFFSLYAKVIFFHYSALVESKYRAITILSSVAVSYGYLWC
                     CIYFGWHVFYAYVFFYLIQALFSFTIYKFYFPYSAKWTIDLELVKMYFFMGSKLIVST
                     ISVSLFTQCDVILLESLTGTKEAGAFSAALRLSAIWFMCGGLIANAFFPKIVQLEKIG
                     EEESFIFLKWICGVVSVISIYGAIIMIALSPIIIKILYGDNMDLSAQVLMVHMWSGVF
                     VFLGSFSSKWLFSKNYINLEVIKTIIAAILNITLNIIVIPKYGAVGAASVSLLSYFIA
                     NFLIFIFIPKTKNMFKMQLQSLKYIIFPWRLINDFGRVRCQFQ"
     CDS             6812..7678
                     /note="O038_06"
                     /gene="wffL"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00860.1"
                     /translation="MSISVITPVFNRALLVYELYESLMSQQSYDFEWVIIDDGSTDNL
                     KEVIDKIASTSPFKIIYRYKKNGGKHTALNIGIEMSSFNWIFIVDSDDILTPNAIALA
                     NEKIQAIVDDKCKGMVFLKGYKTTKEIVGKAETIENISLEKFAGTKGDKALIIKRDSL
                     LKNQFPVFDGENFITEALVWNSILENGYFKYFNEIIYYSEYLPGGLTSNYTDLLRKNI
                     NGTMAFVINNLNLKGLGINVIKQTVFHFIPIFNISNLIVVKKKTKFTVFVLFITCLFL
                     VKIKNKVKGKSL"
     CDS             7675..8361
                     /note="O038_07"
                     /gene="wffM"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00861.1"
                     /translation="MIPAIIHYIWLGKSEIPKIYLDCMESWKEHAVNYDCYLWNEDSY
                     RKEFGQNDFVEEMIQRKKFAFAADLIRCDVLYRFGGIYLDTDMELVRDISALRKNIAF
                     IGEEDIDTPSCGILGCEPKFWLFQELKAAVIKANGMQTIPFLLKNILDLHGVKKIDSQ
                     DISTIKDITIYSDKYFYPYNPYGSAKRSQLLYRYITKDCYAIHHWAKSWKLSFLERIK
                     RKIIMRYRKE"
     CDS             8363..9394
                     /note="O038_08"
                     /gene="wfoE"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-glucose 4-epimerase"
                     /protein_id="BAQ00862.1"
                     /translation="MNILVTGGAGYIGSHTVLRLLENENEITVVDNLVNSSSEVIKRV
                     ENITQKSICFIEMDILNTELLHEVIINKDIDAVIHFAGLKSVSESISRPLEYYKNNVQ
                     GTISVLSAMINSKAKKIIFSSSATVYGEPEQIPLNEKCKVGGTTNPYGTSKLMAEQIL
                     CDFAKANYGFDIISLRYFNPVGAHPSGMIGEAPNGIPNNLVPYLTKVAIGELDSLKIF
                     GNDYPTRDGYGVRDFIHVMDLADGHIAALNAEFKENSIRIYNLGTGKGYSVLELVDTF
                     ERIIARKINKCVISRRDGDIAECWSDPMLAFNELGWSAKFNLEDMLRDSWNWQIKNPK
                     GYDQNKGRK"
     CDS             9397..10143
                     /note="O038_09"
                     /gene="wffN"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00863.1"
                     /translation="MDYLVSIIMPSYNSEFTIKESIKSVIEQTYSNWELLITDDCSTD
                     RTCQIVKEFVEQDDRIKLFVSDKNKGAGAARNNSIKESSGRFLAFLDSDDLWAPDKLK
                     EQINYMIMNGYALTYTAYSKIDAYGNIKKDIQPPSKVDFSSLLKSNVIGCLTAIYDTE
                     VVGKVYMPLIRKRQDMALWLIILQKIDYAHCLNKNLAFYREGHLSLSSNKIKIIKSQW
                     EFYRYYLGFGYVKAMYYFLHYIQRALRKHA"
     gene            10281..11687
                     /gene="gnd"
     CDS             10281..11687
                     /gene="gnd"
                     /note="O038_10"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ00864.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVVEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            11936..13102
                     /gene="ugd"
     CDS             11936..13102
                     /gene="ugd"
                     /note="O038_11"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ00865.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKLSP
                     IVDKEIEEYLATKDLNFRATTDKFDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            13192..14226
                     /gene="wzz"
     CDS             13192..14226
                     /gene="wzz"
                     /note="O038_12"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ00866.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNYDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQT
                     RQNLLDIENLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(14323..14934)
                     /gene="hisI"
     CDS             complement(14323..14934)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ00867.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     4520 a   2730 c   3293 g   4696 t
ORIGIN
        1 atcggtgcca acatcaccaa ctgtaagttc agcgacttac aaggcgacgc cattgaatgg
       61 aacgtggcaa ttaacgacag tgatattttg atctccgacc acatcatcga gcgcatcaac
      121 tgtactaacg gcaaaatcaa ctggggcatt ggcataggtc tcgcgggaag cacttatgat
      181 aataattacc cggaagacca ggcagtgaaa aactttgtcg tggcgaatat cacgggatcg
      241 gattgtcggc agttgatcca tgttgaaaat ggtaaacatt ttgttattcg taatatcaaa
      301 gctcgcaata tcacaccgga tttcagtaag aaagcaggta ttgataacgc gacagtcgct
      361 atttacggtt gtgacaattt cgtgattgat aatattgaaa tgattaatag cgccgggatg
      421 ttaatcggct atggggtaat taaaggcaaa tatctctcga taccgcaaaa tttccgagtg
      481 aataatattc aactggataa cacccatctt gcttataaat tgcgcggcat ccaaatctcc
      541 gccgggaatg ctgtctcctt tgtggcgctg actaacattg agatgaagcg tgcgtcgctg
      601 gagttacaca acaaaccgca acatcttttt atgcgtaata tcaaagtgat gcaggaatcc
      661 tctgttggac cagcattgag catgaacttc gacatgcgca aagacgttcg cggcgtcttt
      721 atggcgaaaa aagaaacact gctgtcactt gcaaatgttc atgcggtgaa tgaaaaagga
      781 caaagctccg tcgatatcga cagagttaat caccatattg ttaatgtgga aaagattaac
      841 tttagattgc cggaacggag agagtagatt tgcgaccatt cataaactta ctggatataa
      901 tgttctaaaa gattataatc ctcacactaa ctgttaaagc tggggctggg cagcattgac
      961 tactcaggga aggtactcat ctttatcttg tgtttcttac gtaacaacca gactaaagtg
     1021 atttatgaac gataacgttt tgctcatagg agcttccgga ttcgtaggaa cccgactact
     1081 tgaaacggca attgctgact ttaatatcaa gaacctggac aaacagcaga gccactttta
     1141 tccagaaatc acacagattg gcgatgttcg tgatcaacag gcactcgacc aggcgttagc
     1201 cggttttgac actgttgtgc tactggcagc ggaacaccgc gatgacgtca gccctacttc
     1261 tctctattat gatgtcaacg ttcagggtac ccgcaatgtg ctggcggcca tggaaaaaaa
     1321 tggcgttaaa aatatcatct ttaccagttc cgttgctgtt tatggtttga acaaacacaa
     1381 ccctgacgaa aaccatccac acgatccttt caaccactac ggcaaaagta agtggcaggc
     1441 agaggaagtg ctgcgtgaat ggtataacaa agcaccaaca gaacgttcat taaccatcat
     1501 ccgtcctacc gttatcttcg gtgaacggaa ccgcggtaac gtctataact tgctgaaaca
     1561 gatcgctggc ggtaagttta tgatggtggg ggcagggact aactataagt ccatggctta
     1621 tgttggaaac attgttgagt ttatcaagta caaactgaag aatgttgccg caggttatga
     1681 ggtttataac tacgttgata agccagacct gaacatgaac cagttggttg ctgaagttga
     1741 acaaagcctg aacaaaaaga tcccttctat gcacttgcct tacccactag gaatgctggg
     1801 tggatattgt tttgatatcc tgagcaaaat tacgggcaaa aaatacgctg tcagctctgt
     1861 gcgtgtgaaa aaattctgcg caacaacaca gtttgacgca acgaaagtgc attcttcagg
     1921 ttttgtggca ccgtatacgc tgtcgcaagg tctggatcga actctgcagt atgaattcgt
     1981 ccatgccaaa aaagacgaca taacgtttgt ttctgagtaa agcagacgag tcagagaaaa
     2041 aaggaccagg ttggactacg attattcgga aagaggtact gattaaatta tcagtatgtg
     2101 gatttccgag taatccgagt aacccagatg ctgatggtgt gtagtgttat cgtaaggttt
     2161 aatcaaagtt atgcggagct aagctttggt atatagccat aacgtttggc taaccgatgg
     2221 caataattta cgcactatca gccacttaga ggtaggaaaa gaatgacgaa tttaaaagca
     2281 gttataccgg tagcaggtct tgggatgcat atgttgcctg ccactaaggc gattcccaaa
     2341 gagatgctac cgatcgtcga caagccaatg attcagtaca ttgttgacga gattgtggct
     2401 gcagggatca aagaaatcct cctggtaact cacgcgtcca agaacgcggt cgaaaaccac
     2461 ttcgacacct cttatgaatt agaatctctc cttgaactgc gcgtgaagcg ccaactgttg
     2521 gcggaagtac agtccatctg tccgcctggc gtgaccatta tgaacgtgcg tcagggcgaa
     2581 cctttaggtt taggccactc cattttatgt gcacgacccg ccattggcga caacccattt
     2641 gtcgtggtac tgccagacgt agtgatcgac gacgccagcg ccgacccgct gcgctacaac
     2701 cttgctgccg tgattgcgcg cttcaatgaa acgggccgta gccaggtgct ggcaaaacgt
     2761 atgccgggtg acctctctga atactccgtc attcagacca aagaaccgct ggaccgtgaa
     2821 ggcaaagtca gccgcattgt tgaattcatc gaaaaaccgg atcagccaca aacgctggac
     2881 tcagacatca tggccgttgg tcgctatgtg ctttctgccg atatttggcc ggaacttgaa
     2941 cgcactcaac ctggtgcatg ggggcgtatt cagctgactg atgccattgc tgaactggcg
     3001 aaaaaacagt ccgttgatgc aatgctgatg actggtgaaa gctacgactg cggtaaaaaa
     3061 atgggctata tgcaggcgtt tgtgaagtat ggattgcgca acctgaaaga aggggcgaag
     3121 ttccgtaaag ggattgagaa gctgttaagc gaataatgaa aatctgaccg gatgtaacgg
     3181 ttgataagaa aattataacg gcagtgagga ttcgtggcga aagtaatttg ttgcgaatat
     3241 tcctgccgtt gttttatata aacaatcaga ataacaacga gttagcaata ggattttagt
     3301 caaagttttc caggattttc cttgtttcca gagcggattg gtaagacaat tagcgtttga
     3361 atttttcgag ttaagcgcga gtgggtaacg ctcgtcacat cgtagacatg catgcagtgc
     3421 tctggtagct gtaaagccag gggcggtagc gttatttata ttcataggtc taaaatagat
     3481 aaccgagatg aaaatagcta taggtatatc aacattaaat aatggaataa atcaggtttg
     3541 ggaaaaaata aaaaatatac cagatgaatt tttaataata atttcacatc aagtgactga
     3601 taagcaaaaa caatatgggg atatttgtaa gaaaaacaat gtgataataa taacgacata
     3661 ctgtaaaggg ttaagtaaaa gcagaaatat tttattggct actgcatttc aaaaaagcgt
     3721 tgattatatg attataagtg atgatgatgt cgcttacctt gttaatggac tttatgaact
     3781 aaaagataga ataatcgaag atagaggcaa gtatcattat caaattcaaa gttgtacgca
     3841 agacggaaga ctaaggaaaa aatatccgct aatgaggaaa aggctaaata gattgtcagc
     3901 atttaacatt tcttcaatag aaatgtgcct taatgtgaat caaatacagg aatgtaatgt
     3961 ttggtttgat gagtgtttcg gattgggggc tagatataaa gcgggtgaag aaccgatttt
     4021 tgttacggat cttatgaaaa caaaaaataa tattattttt atacctgtca caattactgt
     4081 gcatccaata gaaagttctg gtaaaaaaat ttataatgaa acagatgccc tatcagatag
     4141 aagtgctatt tttgtacgct gtggtggtag atatttaggg ttactatata tcttcatatt
     4201 ctgggtgaaa aaatttcttt ttaaaaaaaa atgcggcaga attaaaaaaa tagctgcatt
     4261 atttattctg ctaaaaggat attcccgata tgaatattta taaaaacagc aatgaaaatt
     4321 cattgtcaaa taataactgt attgtatatt ttattccttt gttattattg gcaacattct
     4381 ttttccctct tgttgtattg atatttattg gtgccctctc gccattgtta cacccagtat
     4441 tgcgtaattt ttatttttat gcactattgg tgtttattat tatttttttt tccacactaa
     4501 aaccatttgg agatattgca gagtatcttc atgtttacca tgagttaaat tataatttaa
     4561 ttgatgtttt tggctattca agatttggtg atgggctgga atttatgttt ttagccatca
     4621 tgaaatttat tgggtatatt tcaggtggca atgatgaagt atttttactt tctacttatt
     4681 ttctgatcgt ctttttttta tccaagattc ttaaagatgt tgataaaaaa tataaacttt
     4741 ttttattatc gcttttcttt tttaacttag gatttataga agttacatca tattttcttc
     4801 gacaggtttt atctgtggtt gtttttctgt atgctataaa tgagcgttca tttaaaaaat
     4861 atatcttttt tttgttgagt gttttttttc acatgtctgc agttgttaat gtttttatat
     4921 atgcagttta tatgatcttt ggttcaaaaa aatactcata tgcaaaaatt gttttttcta
     4981 ttcttattgg acttttagtt gtgttttttg tggtttataa tacgccaatt tattctgtgc
     5041 tattatcgaa attcacttca gtttctggga acgataaatt cacacgtttg ccgttaaatt
     5101 atattatcat tacagttgta aatatatgct ttattataat gatagggaag agaagtaaat
     5161 gtgatgattt taataagatt ttattttgca aggagtgttt tctattctta atattattac
     5221 ctttcccagc actttcgaat agattgggta tgattatttt tggtttttat ccctacttta
     5281 tcttacctta tctaaaagct tttgagagaa aaggtaagag taaatatgcc ctacttattt
     5341 gtttatatgt agtaaattta gtgccttttt tgtatttaat gtataatgtg tctttaggga
     5401 ataacatgtt tactttccta aataatcatc catttactga gggtgtgtat ggtatgattg
     5461 attacatatt ggaggcaatt gataaaggtg ttaactatat caatgaaggt aactagcacg
     5521 tgaaaaaata cttaagtaac ctattgtggt tgttgagtga ccgagtgttc atgctggtat
     5581 ttcagttgtc actttttgct tctatcaaaa gaatctatgg gttagatatc ttgggtagtt
     5641 gggcaacgat aatgaatatc tcacaaatat tactatcgtt atttttattt ggcattgata
     5701 tagtcgtagt taaaagaatt gttgaaaatc catcatcaac gggcactgag attggatgtg
     5761 cattattctt acagttttta ggactgttat tatatgcatc tgcttttatt accattgtta
     5821 tttattttta ctatgatata ccgttcgctt ttatattcgt ggcgatatta atcgttgcta
     5881 atttttttag cttgtatgct aaagtaatat tttttcatta ttcggcattg gttgaatcaa
     5941 aatatcgtgc tatcacaatt ctaagtagtg tggccgtttc atatggttat ctttggtgtt
     6001 gtatatattt tggttggcat gtcttttatg cttatgtttt cttttattta attcaagctc
     6061 tttttagttt tactatatac aaattctatt tcccttattc agcaaaatgg acgattgatt
     6121 tagaattagt caaaatgtat ttttttatgg ggagtaaact cattgtatca actattagtg
     6181 tatcactatt tacacagtgt gatgttatct tattagagtc tcttacaggt acaaaagagg
     6241 ctggggcatt tagtgcagct cttaggttat cagccatctg gtttatgtgt ggaggtttga
     6301 tagcgaacgc tttttttcca aaaattgttc agcttgaaaa aataggagag gaagaatcat
     6361 ttatcttttt gaaatggata tgcggagttg taagtgtaat ctctatatat ggtgcaatta
     6421 ttatgattgc tctgtcacct ataattataa aaatactata tggcgataat atggatttat
     6481 cggcgcaagt attaatggtt catatgtgga gtggtgtttt tgttttttta ggatcatttt
     6541 catctaaatg gttatttagt aagaattata ttaatttaga agtgataaaa accattattg
     6601 ctgcaatttt gaatataact ttgaatatca ttgttatacc aaaatatgga gcggttggcg
     6661 ctgcttctgt atcattgctt tcttatttta ttgctaactt tttgattttt atatttatac
     6721 caaaaactaa aaatatgttt aaaatgcaat tacagagttt gaagtatatt atttttccat
     6781 ggcgtttgat taatgatttt ggaagggtta gatgtcaatt tcagtaatta ctcctgtatt
     6841 caatcgagct ttgttagtct atgaattata tgaatcctta atgtcacaac aatcttatga
     6901 ttttgaatgg gtgataattg atgatggttc tactgataat ttaaaagagg ttattgacaa
     6961 aatagcgtcg acatccccat ttaaaatcat atatagatat aagaagaacg gtggaaaaca
     7021 cacagcctta aatataggga tagaaatgtc atcgtttaac tggatattta ttgttgatag
     7081 tgatgatata ttaacgccca acgctattgc tctagccaat gaaaaaatcc aggcaattgt
     7141 tgatgataaa tgtaaaggga tggttttcct gaaggggtat aaaactacaa aagagattgt
     7201 aggtaaagcg gaaacaattg aaaatatttc tctggaaaag tttgcaggta caaagggaga
     7261 taaagcatta atcataaaac gtgattcttt acttaaaaat caatttcctg tttttgatgg
     7321 tgaaaatttt ataactgagg cattggtttg gaattcaatt ttagaaaatg gctactttaa
     7381 atatttcaat gagatcatct attatagtga atatttacca ggaggtttaa cttctaatta
     7441 tactgatctt ttgcggaaaa atatcaatgg cacgatggct tttgttatca ataacttgaa
     7501 tctaaaaggt cttggtatta atgtcattaa acaaaccgtt tttcatttta ttcccatttt
     7561 taatattagt aatctaatag ttgtaaagaa aaagacaaag tttactgttt tcgttttatt
     7621 tattacatgt ctttttcttg taaaaatcaa gaataaagtt aaaggaaaat cgttatgata
     7681 ccggcaataa ttcattatat ttggcttggt aaaagtgaaa tccctaaaat atatttagat
     7741 tgtatggagt cgtggaaaga acatgctgta aattacgatt gctatttatg gaatgaggat
     7801 tcttatagaa aggaatttgg tcagaatgat tttgttgaag aaatgatcca aaggaaaaag
     7861 tttgcatttg ctgcagattt gatccgatgt gatgtattat atcgttttgg tggtatatat
     7921 ctcgatactg atatggaatt agttcgggat atttctgcat tgcgaaaaaa tattgcattt
     7981 atcggcgaag aagatattga tacgcctagt tgtggtattt tgggttgtga acccaaattt
     8041 tggcttttcc aagagctaaa agcagctgtc ataaaagcaa atggtatgca aacaattcct
     8101 tttcttttaa agaatatttt ggacttacat ggtgtaaaaa aaatagattc acaagatatt
     8161 tctactatta aagatatcac aatatactct gataagtatt tttatccata taacccttat
     8221 ggtagtgcca aacgatcaca attactttat agatatataa caaaagattg ttatgctata
     8281 catcattggg ctaaaagttg gaagctttct tttttagaga gaattaaaag aaaaattatc
     8341 atgcgatatc gtaaggagta atatgaatat cctagtaaca ggtggtgctg gttatattgg
     8401 ttcgcatacg gtacttcgct tattagaaaa tgaaaatgaa attactgttg tggataattt
     8461 ggttaattct tcatctgagg taattaagcg tgtagagaat atcacgcaaa aaagcatttg
     8521 ctttatcgaa atggatatac ttaacacaga attattacac gaagtaatta ttaataaaga
     8581 tattgatgcg gtcattcatt ttgctgggtt gaaatctgtt tctgagtcaa tcagtaggcc
     8641 tcttgaatat tataagaata atgttcaggg aacaattagt gtccttagtg ctatgataaa
     8701 tagcaaagcc aagaaaatta tttttagttc ttctgcaacg gtttatggtg agcctgaaca
     8761 aattccatta aatgaaaaat gtaaagtagg tggtacaaca aatccttatg gcacttcgaa
     8821 attaatggca gaacaaatcc tttgtgattt tgcaaaagca aattatggtt ttgatattat
     8881 ttcattacga tactttaatc cggtaggagc acatcccagt ggaatgatag gtgaggctcc
     8941 gaatgggatt cctaataatc ttgtaccata tctcactaag gttgctatag gcgaattaga
     9001 ttcattgaaa atatttggga atgattatcc taccagggac ggatatggag ttcgggattt
     9061 tattcatgtt atggatcttg ccgatggaca tattgctgct ttgaatgcag agtttaaaga
     9121 aaattctatc aggatatata atttaggtac aggtaaagga tattcagtat tagagttggt
     9181 tgatactttc gagagaatta tagctagaaa aataaataag tgtgttatat caaggcgaga
     9241 tggagatata gcagaatgtt ggtctgatcc aatgttagcc ttcaatgaac tcggttggtc
     9301 agccaaattc aatttagagg atatgctacg agattcttgg aattggcaaa taaaaaaccc
     9361 gaaaggctac gatcaaaata aagggaggaa ataataatgg attatcttgt gtcaattatt
     9421 atgcccagtt ataattctga attcacaatt aaggaaagta taaaatcggt aatagaacaa
     9481 acatattcta attgggagtt gttaattact gatgactgct caacagatag aacttgtcag
     9541 atagttaaag aatttgttga gcaagatgac aggataaaac tttttgtttc agataaaaat
     9601 aaaggcgcag gcgcagcaag aaacaactca ataaaagagt ctagtggacg atttttggct
     9661 tttttagata gtgatgatct atgggcaccc gataaactaa aagagcagat taattatatg
     9721 attatgaatg gctacgccct tacttatact gcatatagta aaattgatgc atatggtaat
     9781 attaaaaaag atattcagcc cccatcaaaa gtcgattttt cttctttact gaaatccaat
     9841 gttatcggat gccttactgc aatttatgac acggaagttg tgggtaaagt atatatgcca
     9901 ctcatacgaa agcgtcaaga tatggcatta tggctaataa tcctgcaaaa aatagattat
     9961 gcccactgcc taaataaaaa cctagcattt tatcgcgaag gtcatttaag tttatcatcg
    10021 aataagataa aaataataaa atcgcaatgg gagttttacc gttattatct tggttttgga
    10081 tatgtaaagg caatgtatta ttttctgcat tatatccagc gtgcattaag aaaacacgct
    10141 taagagttta actctatttg tttaatttta aaacattgat aataattgag tctatcattc
    10201 atttattgca tgtcttattt tatatctgtt atccttatta accgtcaaat cccgcggtaa
    10261 ccccctgaca ggagtaaaca atgtcaaagc aacagatcgg cgtagtcggt atggcagtga
    10321 tggggcgcaa cctcgcgctc aacatcgaaa gccgtggtta taccgtctct attttcaacc
    10381 gttcccgtga aaaaacggaa gaagtgattg ccgaaaatcc gggcaagaaa ctggttcctt
    10441 actatacggt gaaagagttt gttgaatctc tggaaacgcc tcgtcgcatc ctgttaatgg
    10501 tgaaagcagg tgcaggcacg gatgctgcta ttgattccct caagccatac ctcgataaag
    10561 gtgacatcat cattgatggt ggtaatacct tcttccagga cactattcgt cgtaaccgtg
    10621 agctttctgc agaaggcttt aatttcattg gtaccggtgt ttccggcggt gaagaaggtg
    10681 cgctgaaagg tccttccatt atgcctggtg ggcagaaaga agcctatgaa ctggttgctc
    10741 cgatcctgac caaaatcgcc gcagtggttg aagacggcga gccatgcgtt acctatattg
    10801 gtgccgatgg tgcaggtcac tatgtgaaga tggttcacaa cggtattgaa tacggcgata
    10861 tgcagctgat tgctgaagcc tattctctgc ttaaaggtgg cctgaatctt tccaacgaag
    10921 aactggcgca gacgtttacc gagtggaata atggtgaact gagcagctac ctgatcgaca
    10981 tcaccaaaga tatcttcacc aaaaaagatg aagacggtaa ctacctggtt gatgtgattc
    11041 tggatgaagc agcaaataaa ggtacgggca aatggaccag ccagagtgcg ctggatctcg
    11101 gcgaaccgct gtcgctgatt accgagtctg tgtttgctcg ttatatctct tctctgaaag
    11161 atcagcgcgt tgccgcgtct aaagttctct ctggcccgca agcgcagcca gctggcgaca
    11221 aggctgagtt catcgaaaaa gttcgtcgtg ctctgtatct gggcaaaatc gtttcttacg
    11281 cccaaggctt ctctcagctg cgtgcggcgt ctgaagagta caactgggat ctgaactacg
    11341 gcgaaatcgc gaagattttc cgtgctggct gcatcatccg tgcgcagttc ctgcagaaaa
    11401 tcactgatgc atatgccgaa aatccgcaga tcgctaatct gctgctggct ccgtacttta
    11461 aacaaattgc cgatgattac cagcaggcgc tgcgtgatgt cgttgcttat gcagtgcaga
    11521 acggtatccc ggttccgacc ttcgccgctg cggttgccta ttatgacagc taccgtgccg
    11581 ctgttctgcc tgcgaacctg atccaggcac agcgtgacta tttcggtgcg catacttata
    11641 agcgcattga taaagaaggt gtgttccata ctgaatggct ggattaatct gatttaaatc
    11701 aattaaccaa cacaaggccc ggagaaaccc tccgggcttt tttattatac aaagcggcag
    11761 gttatggcct ttttttataa tttatgatta aaaaagcgat ataatacaga gccgcacagc
    11821 aggatcgctg tcttgacagt tcaaccgcat cagcgttaaa aatcccgcag tagatgaagt
    11881 tgtggtggtg gactaatgac cactctaaat gtttaaccag aagaagtcag agctaatgaa
    11941 aataacaatt tcaggaacag gttatgttgg tctttcaaat ggtattctga ttgcgcaaaa
    12001 ccacgaagtg gttgcactgg atatcgttca ggccaaagtg gacatgctta acaagaagct
    12061 gtcaccgatt gttgataagg agattgaaga gtatctggcg actaaagatc tcaatttccg
    12121 cgctacgaca gataagtttg acgcgtataa aaatgccgat tacgttatta ttgccacgcc
    12181 taccgattat gacccgaaaa caaattattt taatacctca agcgtggaag cggtcattcg
    12241 cgatgtgaca gaaattaatc ctaatgctgt catggtgatt aaatctacaa ttccggtagg
    12301 tttcaccaaa tcaattaaag aacgtctggg tatcgataac cttattttct ctccggaatt
    12361 cctccgcgaa ggtaaagctc tttacgataa ccttcatccg tcacgtattg tcatcggtga
    12421 gcgttcagaa cgcgcagaac gttttgcggc gttattacag gaaggcgcga ttaagcaaaa
    12481 tatccctaca ctgtttaccg actccactga agcagaagcg attaaactgt ttgctaatac
    12541 ctatctggcg atgcgcgtag catactttaa tgaactggat agctatgcag aaagtttagg
    12601 tctgaatact cgccagatta tcgaaggcgt ttgtctcgat ccacgcattg gcaaccatta
    12661 caacaatccg tcgtttggtt atggtggtta ttgtctgccg aaagatacca agcagttact
    12721 ggcgaactac cagtctgtgc cgaataacct gatctcggca attgtcgatg ctaaccgcac
    12781 gcgtaaagac tttattgccg atgccatttt gtcacgcaag ccgcaagtgg tgggtattta
    12841 tcgtctgatt atgaagagtg ggtcagataa cttccgtgcg tcttccattc aggggattat
    12901 gaaacgtatc aaggcgaaag gcgttgaagt gatcatctac gaaccggtga tgaaagaaga
    12961 ttcattcttc aactctcgcc tggaacgtga tctcgccacc ttcaaacaac aagccgatgt
    13021 cattatttcc aaccgtatgg cagaagagct taaggatgtg gcagacaaag tctacacccg
    13081 cgatctcttt ggtagtgact aacatcctgt tatcagggcg attttcgccc tgattctctt
    13141 aagttccctt tgtaataatt cattattttt atcatttatc ctatagcatt catggcgatt
    13201 atcgctaaac tatggcgact taaaattatt tcgtccgtta gggtaatgat gagagtagaa
    13261 aataataatg tttctgggca aaactatgac ccggaacaga ttgatttgat tgatttactg
    13321 gtgcagttgt ggcgtggcaa gatgacaatt atcatctctg tcattgttgc tattgccttg
    13381 gctatagggt atctggccgt tgccaaagaa aagtggactt ccacagctat cgttactcaa
    13441 cctgatgtgg ggcaaatcgc tggttatacc aacgcgatga atgtgattta cggatcggct
    13501 gtaccgaaag tctccgacat tcaggcttcg cttatcggac gttacagcac tgcattctca
    13561 gcattagcgg aaacgctgga taaccaggaa gaagcagaga agctgaccat tgagcctacc
    13621 gtcaaaaatc agtcattacc tttagcggtg tcttatgtgg gtgaaacacc tgaaggtgcg
    13681 cagaaacagc tggcgaagta tatccagcaa gttgacgacc aggtaaatga agaactggaa
    13741 aaagacctga aagacaacat cgcgctgcgt atgaaaaact tgcaggattc tctgaaaact
    13801 caggaagtag ttgcgcagga gcagaaagat ctgcgtatcc gtcagattca ggaagcgttg
    13861 cagtatgcga atcaggcgca ggtgacaaag ccacaggttc agcagactga agatgtgacg
    13921 caagatacat tgttcctttt ggggagcgaa gcgctggagt cgatgattaa gcatgaggcg
    13981 acccgtccgt tggtgttctc accaaactac tatcagactc gtcaaaacct gctggatatc
    14041 gaaaacttaa aggttgatga tcttgatatt catgcttacc gctatgtaat gaaaccgacg
    14101 ttacctattc gtcgcgatag tccgaaaaag gcaatcacct tgattctggc ggtgctgctg
    14161 ggcggcatgg ttggcgcggg gattgtgctg gggcgtaacg ctctgcgtaa ttacaacgcg
    14221 aagtaatctt ttcggtttta aagaaaaagg gcagggtggt gacaccttgc ccgttttttt
    14281 tgccggatgc gatgttgttg tcgcatccgc ttaccccgca actcactgat gccgtttgcg
    14341 caggttctca attaccgtcg ttaaatccag atcctgatcc tgcaacagca ccagcaggtg
    14401 atacatcaaa tcagacgcct cgttggtcag ctcaaagcgg tcatgtacag tcgcggccag
    14461 tgcggtttcc acgccttctt cgcctacttt ctgcgcaatg cgcttggtgc cgctggcata
    14521 cagtttggca gtgtaggagg tttccgggtc ggcagattta cgttcggcga gcagttgttc
    14581 cagttgatac aggaacagcc actggtgagc ggtgtcgccg aagcagctgc tggtgccttt
    14641 gtggcaggtt ggcccgatgg gattcgccag caccagtaac gtgtcgttgt cgcagtccgg
    14701 cgcaatactc accacattga ggaaattgcc cgacgtttcg cctttggtcc acagccgttg
    14761 tttagtgcgc gagaagaagg tgactttgcc gctttcgatg gttttatcta aggcttccgg
    14821 gttcatatag cccagcatta acacttcgcc agataccgcg tgttgcacaa tcaccggcat
    14881 aagtccgtcg gttttttccc agtccagttc gcgacgttgt tgttctgtta acatatcctg
    14941 atctccacgc cctgtgttgc caggtacgct tttaattcac caatattgat tatttgtttg
    15001 tggaatacgg aagcagccag cgcgccgtca acgtcggcat cgcggaaggc ttcgaggaag
    15061 tgttccatgg tgcccgcgcc accggaagca atcagcggga cgtggcaaac ttcacgcact
    15121 tttttcagtt gttcgaggtc gtaaccgtta cgcacgccgt cctgattcat catgttgagg
    15181 acgatttctc cggcaccgcg tttttgcact tcctctaccc agtcgagcgt ttcccactg
//
