LOCUS       EU296417               13698 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serogroup O40 O-antigen gene cluster, complete
            sequence.
ACCESSION   EU296417
VERSION     EU296417.1  GI:187880607
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13698)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 13698)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..13698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O40"
     gene            2431..3600
                     /gene="wffO"
     CDS             2431..3600
                     /gene="wffO"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffO"
                     /protein_id="ACD37115.1"
                     /db_xref="GI:187880608"
                     /translation="MKSVNTKIKIAHLQLMPLLSGVQRVSLQEIENLPPEYFEIDLIC
                     KEGGPLVDALNKKVRKFFIPTLCRNISPVEDLKSLISLYKIFKRERYDIVHTHSSKTG
                     ILGRIAARMARVPCVVHTVHGFAFESTKKQAIKNLYKWLEMIGAKCSTKIICLHEEDK
                     NICLNILKIKADKIVVIPNGVDINKFTPATNKGKIKEEILSLRESNFVFTMVGRLWPQ
                     KNPLYFVEVAKQIIKNELIPGSIFVLVGDGELMSVIKEHYLEDELLHNILLLLGWRND
                     ISDSLKARDVFVLPSLWEGMPLAILEAQSTGLPWVVSNINGNKSLVTNKFDGYLVELN
                     DINSFINALVNISEKNVYARMSNNCRAKIVSNYNITQRIEKIKIMYYQLLNVNMN"
     misc_feature    2431..12265
                     /note="O-antigen gene cluster"
     gene            5652..6404
                     /gene="wffP"
     CDS             5652..6404
                     /gene="wffP"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffP"
                     /protein_id="ACD37116.1"
                     /db_xref="GI:187880609"
                     /translation="MKCFVVSLKNSDRRKDFDHRFKIFNYQFFDAITECTSNQFNSAI
                     AKSIYGRCLRKGEIGCSLSHFNIIRDFALNSKENWLLIMEDDAVPESQFIHFINEFEN
                     KHVTTNAEVILLGHSKTSRKHIFVQRLKQPLRNYKNINGISFGENKNVTLCGTVAYLI
                     NKQAANIISGNSQPYWLADDWFLFQNMGIRVYHPQIPLVYEDLSYDSSTENDIYYHHN
                     FIKEPLKNIIHIIIGQYKFWNDSRKLRKSNNK"
     gene            6401..7606
                     /gene="wzy"
     CDS             6401..7606
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACD37117.1"
                     /db_xref="GI:187880610"
                     /translation="MSHFGGGLMKSVNKFNFYLAIFIGMFDLTIFSTGNSLGQALTTV
                     LLLFSILSDINKVFLKPFFFVISYIVLALLVSLFHGETSYHYSFIIVKTGLILFNAMV
                     ISQILILYADKKQISNFVNFIFLIQTISLLLYSNELFQMMRGFFLSARSEHLYENFGD
                     SYWYRDATISNQGYSGYALGALPLVIIAVHNIMKSKFFSIRYFVAVVCCGLGVSIAML
                     NGRSVFLFIPLYMIYLMLRPKEICLIYKIIPVILIIVSAIIIALTNLIYPSNFVLAFF
                     EQKGKSYGIDSVDDLMYNHTILHFDITLFGLGKYLLSDGSAFVRSDIGYYRQMGVGGI
                     FLVLFYIFSIWLAVRYVIYRDLSLFILFIILLVNFKQEAFNNAMLMYGAFLLAAYWVA
                     RDKIEITKK"
     gene            7603..8721
                     /gene="wffQ"
     CDS             7603..8721
                     /gene="wffQ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffQ"
                     /protein_id="ACD37118.1"
                     /db_xref="GI:187880611"
                     /translation="MKIILWHLGRKGAGPKYNLEMALALKKEGYDVYACISKQAENAN
                     DYKKHNINHLSLNTYTGGVSALINSLRLPWLINEFKDYLKINRITHVYCTMPHIWNFF
                     FSKRFKKLDVSYLLTIHDAELHSGEENKLIDILMKKDRKNADGIVVLTKHVKEQLKNL
                     GKSIFIIPHPAFSFNNNKNRAKSIVPDQKIKLLFFGRIHHYKGLDILIDAFEILNKKN
                     NRYSLSIYGSGNISPYQKKILELSNITVHNRWIDDVEIPSIISSHDVCVIPYRDASQS
                     GVIPTVMTCGVPLVVTPVAGLREQVVHLQTAIFAEEVSSDSVARAIDSLVKDISLYEK
                     ISKNALLYAQEEMGWEKAAYRSVEALISITHNSKKYNQ"
     gene            8803..10083
                     /gene="wzx"
     CDS             8803..10083
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACD37119.1"
                     /db_xref="GI:187880612"
                     /translation="MFQNGVYISFYSFIAKATVFLCGPLTLLLISHKLSTEEIAFYYT
                     FFNLIALQQLVELGLGFTLKQCISHAYKIENDVWLYDSKKIVKSYIQFGLYWFIGVSC
                     IIIFVVGPLGLIFYGDYEGSIKWQGAWLGTVGVLAFSIILVPIQIAIEATQNQIANIS
                     LSSYIFSHNSLSLWVFIYNDFKLYSIPLSMLIATYFLLIALKNHYIQLYNKLAFVKEM
                     DKFWTIFSKLWPLFSRVAIVWGVGFLLWNGFNLISFKIFSPEEAGRIIFSITLARAGF
                     TIAESIISAQTTVVSNMISKGENTEAAKYYRKYQLISFAVLIIGYGFYYVVKCFFSDF
                     YLFDKLMPNSFITSVFVFFVLLLILTTNSNFIRCYKIEPFVKVSVWHSLGVPMSFLLS
                     SLYFPMYLYPCSFVLFISIIWSQLISKRILSKIG"
     gene            11438..12265
                     /gene="wffR"
     CDS             11438..12265
                     /gene="wffR"
                     /note="remnant of pyruvyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffR"
                     /protein_id="ACD37120.1"
                     /db_xref="GI:187880613"
                     /translation="MYSNVGDLLIYFGTEAFIKEYNLNVIYRAGMNVDLKKLKDVDVI
                     LFQGGGNFGGLYSHHQQLREKITLKLPNKKIICLPQTLHFYNEEELERSAKIFKKHSD
                     FHLYVRDQESLTIGYQFTDNIKLIPDMAHSLHPLIDINECDINRESYRSPYVRSDKEA
                     NSNNANNMLSKRSFDWANIISPSMYLMSRSIIILDKLRYSNSMKFWDKIASDMVFKSI
                     NYFMSHNEVYSGRLHGIILAALLGKKIRCFDNNYKKNSRYISCWLKEYPSLKNASDF"
BASE COUNT     4247 a   2340 c   2884 g   4227 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgagcagcg cgtgaagcgt
      121 caactgctgg cggaagtgca gtccatctgt ccaccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attttatgtg cacgacctgc tattggtgac
      241 aacccatttg tcgtggtact gccagacgtt gttattgatg acgccagcgc cgacccgctg
      301 cgttacaacc ttgctgccat gattgcgcgc ttcaacgaaa cgggccgcag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactctgtca tccagaccaa agagccgctg
      421 gaccgcgaag gtaaagtcag ccgcattgtt gaattcatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaactagaac gcactcaacc aggtgcatgg ggacgtattc agctgactga tgctattgct
      601 gaactggcaa aaaaacagtc tgttgatgca atgctgatga ctggtgacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttc gtgaagtatg gattacgcaa cctcaaagaa
      721 ggggcgaagt tccgtaaagg cattgagaag ctgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt tgataacaaa attataacgg cagtgaagat tcgtggagaa agtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aagattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgtcacatc gtaggcatgc
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagca tgggtatttt gaagcattta
     1081 tacctcctta ttcagtgaga agggacagat tacgtaacac tgctttgttg tactaaataa
     1141 ttcgcatttt atgttcaaca atcgagatat tccttattac ctaaagctgt ttttcattgt
     1201 ttatacatga tcgaatactc cttacataaa taaggagaac aaaatggaac ttaaaaaatt
     1261 gatggaacat atttctatta tccccgattc cagacaagtc tggaaagtgg aacataaatt
     1321 atcggatatt ctactgttga ctatttgtgc cgttatttct ggtgcagaag gctgggaaga
     1381 tatagggttt ttgggaaact catctcgatt ttttgaagca atatggtgat tttgaaaatg
     1441 gtattcctgt tcacggtacc attgccagag ttatatcctg tatcagtcct gcaaaatttt
     1501 acgagtgcct tattaactgg atgcgtggat gccattcttc aaatgataaa gacgtcatcg
     1561 caattgatgg aaaacactcc ggcaatctta tgacaagagt cgtcgcaagg gagtgattca
     1621 tgtcattagt gcgttcttaa caatgcacag tctggttcag gacagatgag aaatccaatg
     1681 agattacagc tatccctgag cttattaaca cgttggatat taaaggaaaa attatcacaa
     1741 ctgatgcgat gggttgcctg aaagatattg ctgagaagat acaaaaacag ggaggtgatt
     1801 atttattcgc tgtacacgga aaccaggggg gcttaataaa gcctttcagg aaaaatttcc
     1861 gctgaaagaa ttaaataatc cagagcatga cagttacgca atgagtgaaa agagtcacgg
     1921 cagagaagaa atccgtcttt atattgtttg cgatgtccct gatgaactta ttgatttaac
     1981 gtttgaatgg aaagggctga aaaaattctg cgtggcagtc tcctttcggt caataatagc
     2041 agaacaaaag aaagagccag aaatgacgtt cagatattat atcagttctg ctgatttaac
     2101 cgcagaaaag ttcgccacag caatccgaaa ccactggtac gtggagaata agctgcactg
     2161 gcgtctggat gtggtaatga atgaagacga ctgcaaaata agaagaggaa acgcagcaga
     2221 attattttca cggatgcggt acatcgctat taatattttg acgaatgata aggtattcaa
     2281 ggcagggtta agacgtaaga tgcgaaaagc agccatggac agaaactacc tggcgtcagt
     2341 tcttgtgggg aacgggtttt cgtaattttg ccatgggtgc taagaaagaa acgagaatat
     2401 tattattcca tagattggat ctatcataag atgaaatcag ttaatactaa aattaaaatt
     2461 gcacatctac aattaatgcc cttgttaagt ggtgtccagc gagtatcttt gcaggaaatt
     2521 gaaaatttac ctcctgagta ttttgagatt gatttgatat gtaaagaggg tgggccgtta
     2581 gttgatgcgc taaataaaaa agttagaaag ttctttatcc cgactctttg tagaaatatt
     2641 tctccggttg aggatttaaa atccttgatt agcttatata aaatattcaa aagagaaaga
     2701 tatgatattg tgcatacaca ctcttcgaaa acaggtattc taggaagaat agctgcacgt
     2761 atggctagag ttccgtgtgt tgtgcataca gttcatggtt ttgcatttga aagtacgaaa
     2821 aaacaggcaa taaaaaatct atacaaatgg ctggagatga ttggggcaaa atgctctaca
     2881 aaaataatat gtctccatga ggaagacaaa aatatatgtt taaatatact aaaaataaaa
     2941 gcagataaaa ttgtagttat tccaaatggt gttgatataa ataagtttac tccagcaacg
     3001 aataaaggta aaataaaaga agaaatatta agcttgagag aatcaaattt tgtttttact
     3061 atggtaggac gattatggcc acaaaaaaat cccttatatt ttgttgaggt tgctaaacaa
     3121 attataaaaa atgaactaat acctggttcg atttttgtac tcgttggtga tggtgaattg
     3181 atgagtgtaa taaaagaaca ttatcttgaa gatgagttgt tacataatat attgttatta
     3241 ttaggttggc gaaatgatat ctcagatagt ttgaaagcta gggatgtatt cgtactccct
     3301 tctctttggg aaggaatgcc attggccata ctggaagccc aatcaacagg gttaccatgg
     3361 gtagtatcta acattaatgg aaataaatca ttagttacga ataaattcga tggctacttg
     3421 gttgaactta acgatattaa tagctttatc aacgcactag ttaatattag tgaaaaaaat
     3481 gtttatgcca gaatgtctaa taattgccgt gcaaaaatag ttagtaatta caatataaca
     3541 caaagaattg aaaaaataaa aatcatgtat tatcaactac taaatgtaaa catgaactag
     3601 catgtttttg taatgtatgt ttatttcata taaggttgca gggaaagaat atcagaagta
     3661 cactttttgt actgaacaat tcgcatttta tgttcaataa ttgagatatt ccttattacc
     3721 taaagctgtt ttccattgtt tatacatgat cgaatactcc ttacataaat aaggagaaca
     3781 aaatggaact taaaaaattg atggaacata tttctattat ccccgattcc agacaagtct
     3841 ggaaagtgga acataaatta tcggatattc tactgttgac tatttgtgct gttatttctg
     3901 gtgcagaagg ctggggtgat atagagggtt ttggggaaac tcatctcgat tttttgaagc
     3961 aatatggtga ttttgaaaat ggtattcctg ttcacgatac aattgccagg gttgtatcct
     4021 gtatcagtcc tgcaaaattt cacgagtgct ttattaactg gatgcgtgac tgccattctt
     4081 caaatgataa agacgtcatc gcaattgatg gaaaacactc cggcattctt atgacaagag
     4141 tcgctgcagg ggagcgattc atgtcattag tgcgttctca acaatgcaca gtctggtcat
     4201 cggacagatc aagacagatg agaaatccaa tgagatcaca actatccctg aacttcttaa
     4261 catattggat attaaagcag ggcaagatcg tgaaaagcgt gatataagtc actcttttta
     4321 tcctggtttt aactctcacg ggtgattaaa atttaatctt tggaataact aaaaagataa
     4381 aaggacaccc agatgagtat tcaaagtttg cttgattata tttctgtgat ccctgatgga
     4441 cgacaacaag gaaagttaga cataaattat ctgatatttt gctcctcacc gtctgtgcag
     4501 taattgctgg tgccgatgag tggcaggaaa ttgaagattt tggacatgaa agactggaat
     4561 ggctaaagaa atatggtgat tttgctaatg gcattccggt ggatgacacc attgcacgcg
     4621 ttgtgagtaa cattgacagt ttggcctttg aaaagatgtt tattgaatgg atgcaggaat
     4681 gccatgaaat cactgatggc gaaattatag caatagatgg aaagaccata agaggctcct
     4741 ttgataaggg aaaaagaaaa ggagcaatcc atatggtgag tgcattctcg accgaaaatg
     4801 gtattgtact ggggcaggtg aaaacggaag ccaaaagtaa tgaggttaca gccattccag
     4861 agttgcttaa cctactggat ttaaagaaaa gtttgataac cattgatgct atgggctgtc
     4921 agaaagatat cgcttctaaa attaaagata aaaaggcaga ttatcttctg gcagtaaaag
     4981 gcaatcaggg gaaattacat catgcattcg aagaaaaatt ccctgtaaaa atgttctcta
     5041 attataaagg cgattcattt gatactcagg agataagcca tggaagaaaa gaaacacgtt
     5101 tacatattat cagtaacgta acgcctgaat tttgtgattt tgaatttgaa tggaagggat
     5161 taaaaaagct ttgtgtggca ttgtcattca ggtaaaagaa agaagataaa tcagcggaag
     5221 gtttaatcat tcgatattac atttcatcaa aggatatgga tgctaaagaa tttgcacatg
     5281 ctatcagagc tcactggaaa atcgagaata atcatcattg ggtgctcgat gtaaaaatga
     5341 atgaagatgg aagcagaata agaagaggaa acgcagccaa aagaatatct ggaataaaga
     5401 aagtggccct gaatttattg agagattgca aaggttttaa gggcggggta aagagaaaaa
     5461 gaaagaaggc tgcgttaaat acaagttaca tagagaaagt aattgcatcc tgcgcaatgc
     5521 ctggatttcg gtctgacaaa atgaaaaatt taacacaaat ttaattcatg ctcttgccct
     5581 gcggttgtgg aagtttgaac tatagaaata cttaatcaac ttaagttcta agtataggaa
     5641 atgaatagaa catgaaatgt tttgtagtgt cattgaagaa tagtgatcgt agaaaagatt
     5701 ttgaccatag atttaaaatt tttaattatc aattttttga cgctatcact gaatgcacaa
     5761 gtaatcaatt caactcagct attgcaaaat caatatatgg aaggtgcttg agaaaagggg
     5821 agattggttg ttcgttgagc cattttaata ttattagaga ttttgcatta aactccaaag
     5881 aaaattggtt gctaatcatg gaggatgatg ctgttccaga gagtcaattt attcatttta
     5941 taaatgaatt tgaaaataaa catgtaacta caaacgctga agtaatatta ttaggtcatt
     6001 caaaaacaag ccgtaagcat atatttgttc aacgtttgaa gcagccactg cgtaactata
     6061 aaaacataaa cggaataagt tttggtgaaa ataaaaatgt taccctttgt gggacagttg
     6121 catatctaat taataaacaa gccgccaaca taatatctgg taatagccaa ccgtattggc
     6181 ttgctgacga ttggttctta ttccaaaata tggggattag ggtttatcat ccacaaattc
     6241 cgctagtgta tgaagatctc tcttatgata gctctacaga aaatgatatt tattaccatc
     6301 acaattttat aaaagaaccc ctaaaaaata tcatacacat aatcataggg caatataaat
     6361 tctggaatga ctctcgaaag ttacggaaat caaataataa atgagtcatt tcggaggggg
     6421 gcttatgaag agtgtaaata agtttaattt ttacttggct atttttatag gcatgtttga
     6481 tttaactatt tttagcacgg gtaatagctt agggcaagca cttacaacgg tattgttatt
     6541 attttccata ttaagtgata tcaacaaggt ttttttgaaa ccattttttt ttgttatttc
     6601 atacatagtt cttgcgctac tggtttcttt atttcatggg gagacatcgt atcattattc
     6661 atttattatt gttaaaacag ggcttattct gttcaatgca atggtaattt cacaaattct
     6721 aattttgtat gctgataaaa aacaaatttc aaattttgtt aattttattt ttctaatcca
     6781 gaccatttca ttattattat attcaaatga acttttccaa atgatgcgcg gtttttttct
     6841 ttcagcccgc tcagagcatc tatatgaaaa ttttggtgat tcatattggt acagagatgc
     6901 aacaatctca aatcaaggat attctggata tgcattaggc gcacttcctt tagtaattat
     6961 tgccgtccat aacataatga aatcaaaatt ctttagtatt agatattttg ttgcagtagt
     7021 gtgttgcgga ttaggggtgt caattgcaat gttaaatggt cgttcagtat ttttgtttat
     7081 cccattatat atgatatatc ttatgctgag gcctaaagaa atatgtctga tatataaaat
     7141 aattcctgtt attctgatta ttgtaagtgc aattattatt gcacttacaa atttgatata
     7201 tcccagtaat tttgttttgg ctttttttga acaaaagggt aaatcatatg gcatagattc
     7261 agttgatgat ttaatgtata accatactat cttacatttt gatataacgt tatttgggtt
     7321 gggaaaatat ctactttccg atggttcagc atttgttaga tctgatattg gatactatag
     7381 acaaatggga gtcggtggaa tatttttggt gttattttat attttcagca tatggttagc
     7441 tgtaagatat gttatctatc gtgatttgtc attatttatt ttattcataa tattactggt
     7501 taattttaag caagaagcat ttaataatgc aatgttaatg tatggtgctt ttttactagc
     7561 agcatattgg gtagctcggg ataaaataga gataacaaag aaatgaagat aattttgtgg
     7621 catttggggc gtaaaggtgc tgggccaaaa tataacttag aaatggcact agctttaaaa
     7681 aaagaaggct atgacgtata tgcttgtatt tccaagcagg cggaaaatgc aaatgattat
     7741 aagaaacata acattaatca cttatcgctt aatacatata cggggggtgt ttccgcatta
     7801 atcaattctc tgcgattacc ttggttgatt aatgaattta aagattattt aaagataaat
     7861 cgtattacgc atgtttattg tacaatgccg catatatgga atttcttctt ttctaagaga
     7921 tttaagaaat tagacgtatc ctatttgctc acaattcatg atgcagaatt acatagtggt
     7981 gaggaaaata aattaattga tattttgatg aaaaaagata gaaaaaatgc agatggaatt
     8041 gttgttctta caaaacatgt aaaagaacaa ttaaaaaatt tagggaaatc aatttttata
     8101 attccacatc cagccttttc atttaataac aataaaaaca gagcgaaaag tattgtaccc
     8161 gaccaaaaaa taaaattgct tttttttggt agaattcatc actacaaagg gctggatatt
     8221 cttattgatg catttgaaat actgaacaaa aaaaataaca ggtatagtct ttctatctac
     8281 ggtagtggga atatctctcc atatcaaaag aaaattctag aactaagcaa tattacagtg
     8341 cataatcgtt ggatcgatga tgtagaaatt ccttcaatca tctctagcca tgatgtctgt
     8401 gttattcctt atcgggatgc aagtcagtca ggagtaattc ctacggtaat gacttgtggt
     8461 gtgcctcttg ttgtgacccc ggttgctggg cttagagagc aggttgttca cttacaaaca
     8521 gcaatatttg cggaagaggt ttcttctgat tccgttgcaa gagcaataga ttcattggtt
     8581 aaggatatat ctttgtatga aaagatatca aagaatgctc tactatacgc tcaggaagag
     8641 atgggatggg aaaaagccgc atatcgttct gtggaagctc ttatctcaat tactcataat
     8701 tctaaaaagt ataatcaatg atatctactt catatgcatg atagtactat tgtattcatg
     8761 gcgacaagtt ataaatattg gtaatttaat gttaggtgat agatgtttca aaatggcgtc
     8821 tatatatctt tttattcttt cattgcaaag gctacggtat ttctatgtgg acctttaaca
     8881 ttacttctga tctctcataa attaagtact gaagagatcg ctttttatta taccttcttc
     8941 aatttaatcg cgttacaaca acttgttgaa ctaggtttag gttttactct aaaacagtgt
     9001 atatcacatg cttataaaat tgaaaatgat gtatggttat atgattcgaa gaaaatagtt
     9061 aaatcatata tacaatttgg tttgtactgg tttattgggg tgtcgtgtat tattatattt
     9121 gtcgttggac cattgggatt aatcttctat ggtgattatg aaggtagtat taagtggcag
     9181 ggggcttggt taggaactgt aggtgttctt gcatttagta taatactggt gccaatacaa
     9241 attgctattg aagcaacaca aaatcaaatt gcaaatatat cgttgtcaag ttatatattc
     9301 tctcacaata gtttatctct ttgggtattc atttataatg actttaagtt atattccatt
     9361 cctctaagta tgcttatagc aacatatttt ttgttgattg cactaaaaaa tcattatata
     9421 caactttata ataaattagc ctttgttaaa gaaatggata aattctggac catcttttct
     9481 aaactttggc ctttattttc tagggtggca atagtatggg gtgttggttt tctattgtgg
     9541 aatgggttta atttaatctc attcaaaatt ttttcgcctg aagaggctgg gagaattatc
     9601 tttagtatta cgttagctcg tgccggattt actatagcag agtcaattat tagcgcacaa
     9661 actacagttg tttcaaatat gatttctaaa ggagaaaata ctgaagctgc taagtattat
     9721 cgaaaatatc aactgatttc ctttgctgtt ttaattatag ggtacggctt ttattatgtt
     9781 gtgaagtgtt ttttttctga tttttacttg tttgacaaat taatgccaaa ctcatttata
     9841 acaagtgtat ttgttttttt tgtattgttg cttatcttga caacaaatag caattttatt
     9901 cgttgttata aaattgaacc tttcgttaag gtttctgttt ggcatagtct tggtgtgcca
     9961 atgagttttc ttttatcatc gctttatttc ccgatgtatt tatatccttg ttcctttgtt
    10021 ctgtttatat caataatttg gtctcagtta atttctaagc gcatactttc aaaaataggg
    10081 taaaaaatga ttaataattg gtttacattg atagatgatc ctacccacgt aatatggaca
    10141 caggcctaag cgaggttctg gttttcaaat tgttccggac tgaggccgcc acaccaactg
    10201 tgccgccgcc accgattgta atcacattcg atataattaa ataccgttgc ccgcattatt
    10261 tcccggctga taaagtgttc tccatggata cattccactt tcagcgaatg aaagaagctt
    10321 tccacgcagg cattatcgta gcagcaacct tttgcgctca tacttccacg cagattatgc
    10381 cgcttcagtt gcgcctgata atctgctgaa cagtactggc ctccacggtc cgtgtgaacg
    10441 ataacgttcc ggggcctctt acgccgccac agcgccatct gcagggcatc gcaggccagt
    10501 tgcgccgtca tgcgtggcga cattgaccag ccaataacgg cacgtgacca caggtcaatg
    10561 accactgcca gatacagcca gccttcatct gtacgtaagt acgtgatgtc tcctgcccac
    10621 ttctggttcg ggccactggc gtaaaaatcc tgctccaaca gattttctga cacaggcagg
    10681 ccgtgtgcgc ggtagctgac cgggctgaac ttccgggagg cctttgccct cagtccctga
    10741 cggcgcaggc ttgccgccac ggtttttacg ttaaaggggt aaccctgagc acgcagttca
    10801 tccgtcaggc gtggggcacc gtaacgctgt tttgaccggg taaaagccgc gaggacaacg
    10861 ctttcgcagt gttggcggaa ctgctgacgc gtgcttatcc ttgtccgccg ctgacaccac
    10921 gtataccagc cgctgcgggc cacccggagc acgcggcaca ttgctttgat gctgaactca
    10981 gcctgatgtt tttcaataaa gacatacttc atttcaggcg cttcgcgaag tatgtcgcgg
    11041 ccttttggag gatagccagc tcttcatccc gttctgccag ctggcgtttg agacgtgcaa
    11101 tctcggtaga catctccagt tcacgttcag aagacgtctg ctgattttgc tgtttactgc
    11161 gccagttgta gagctgtgat tcatacaggc tgagttcacg ggctgcggca gtaacaccga
    11221 tgcgttcagc aagcttcagg gcttcactgc gaaattcagg cgaatgctgt ttacggggtt
    11281 ttttactggt tgatactgtt tttgtcatgt gagtcacctc tgactgagag tttactcact
    11341 tagccgcgtg tccactattg ctgggtaaga taaaaatagc cattcggaaa ttgctaattt
    11401 aataggtgaa gctagaatag cttttataga tattccgatg tattccaatg ttggtgattt
    11461 attgatatat tttggtacgg aggcatttat aaaagaatat aacttaaatg ttatttatcg
    11521 cgcaggcatg aatgttgact taaaaaaact aaaggatgta gatgttattt tgtttcaagg
    11581 tggaggcaat tttggtggtt tatattcaca tcatcaacaa ttacgagaga aaataacatt
    11641 aaaactcccg aataaaaaaa taatatgttt gcctcaaacg ttacattttt ataatgaaga
    11701 agagctagag cgatctgcta aaatatttaa gaagcactca gattttcatc tctatgtaag
    11761 agatcaggaa agcctcacta ttggttatca gtttacggat aatataaaac ttatacctga
    11821 tatggcgcat agcttgcatc cgctcataga cattaatgag tgtgatataa acagagaaag
    11881 ttaccgttct ccatatgttc gttctgataa agaagcaaat tcaaataatg ctaataatat
    11941 gctaagtaag cggagctttg attgggcgaa tatcataagc ccaagtatgt atttgatgag
    12001 cagaagcata atcattttgg ataagttgcg ctatagtaat agtatgaagt tttgggataa
    12061 aatagctagc gacatggtgt ttaaatcaat taattatttt atgtcccata atgaagttta
    12121 ctccggcaga ctgcatggta tcattttggc agctttgtta gggaaaaaaa taagatgctt
    12181 tgataataat tataaaaaaa atagtcgtta tatttcttgc tggctaaagg aatatccaag
    12241 cttaaaaaat gctagtgatt tttaatttca gctaataaat ttcatatata ttagaaatac
    12301 attatacaaa tatatatatc cgcatatcgc gcggtaaccc cctgatagga gtaaacaatg
    12361 tcaaagcaac agatcggcgt cgtcggtatg gcagtgatgg ggcgcaacct tgcgctcaat
    12421 atcgaaagcc gtggttatac cgtctctatt ttcaaccgtt cccgtgggaa gacggaagaa
    12481 gtgattgccg aaaatccagg caagaaactg gttccttatt atacggtgaa agagtttgtt
    12541 gaatctctgg aaacgcctcg tcgcatcctg ttaatggtga aagcaggtgc aggcacggat
    12601 gctgctattg attccctcaa gccatacctc gataaaggtg acatcatcat tgatggtggt
    12661 aataccttct tccaggacac cattcgccgt aatcgtgagc tttctgcaga aggctttaac
    12721 ttcattggta ccggtgtttc cggaggcgaa gaaggtgcgt tgaaaggtcc ttccattatg
    12781 cctggtgggc agaaagaagc ctatgagctt gttgcgccga tcctgaccaa aatcgccgca
    12841 gtggctgaag acggcgagcc atgcgttacc tatattggtg ccgatggtgc aggtcactat
    12901 gtgaagatgg ttcacaacgg tattgaatac ggcgatatgc agctgattgc tgaagcctat
    12961 tctctgctta aaggtggcct gaacctcacc aacgaagaac tagcgcagat ctttaccgag
    13021 tggaataacg gtgaacttag cagctacctg atcgacatca ccaaagatat cttcaccaaa
    13081 aaagatgaag agggtaacta cctggttgat gtgattctgg atgaagcagc aaacaaaggt
    13141 acgggcaaat ggaccagcca gagtgcgctc gatctcggcg aaccgctgtc gctgattacc
    13201 gagtctgtgt ttgcacgtta tatctcttct ctgaaagatc agcgcgttgc cgcttctaaa
    13261 gttctctctg gcccgcaagc gcagccagca ggcgataagg ctgagttcat cgaaaaagtt
    13321 cgtcgtgcgc tgtatctggg caaaatcgtt tcttacgctc agggcttctc tcaactgcgt
    13381 gctgcgtctg aagagtacaa ctgggatctg aactacggca aaatcgcgaa gattttccgt
    13441 gctggctgca tcatccgtgc gcagttcctg cagaaaatca ccgatgcata tgccgaaaat
    13501 ccgcagatcg ctaacctgct gttggctccg tacttcaagc aaattgccga tgactaccag
    13561 caggcgctgc gtgatgtcgt tgcttatgca gtacagaacg gtatcccggt tcctaccttc
    13621 gctgctgcgg ttgcctatta cgatagctac cgtgccgctg ttctgcctgc taacaatggt
    13681 ccaggcacag cgcgacta
//
