LOCUS       FJ539194                8027 bp    DNA     linear   BCT 06-JUL-2010
DEFINITION  Escherichia coli strain P11a(O42:K-:H37) O-antigen gene cluster,
            partial sequence.
ACCESSION   FJ539194
VERSION     FJ539194.1  GI:217426215
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Fratamico,P.M., Yan,X., Liu,Y., DebRoy,C., Byrne,B., Monaghan,A.,
            Fanning,S. and Bolton,D.
  TITLE     Escherichia coli serogroup O2 and O28ac O-antigen gene cluster
            sequences and detection of pathogenic E. coli O2 and O28ac by PCR
  JOURNAL   Can. J. Microbiol. 56 (4), 308-316 (2010)
   PUBMED   20453897
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   Fratamico,P.M., Yan,X., DebRoy,C., Bryne,B., Monaghan,A., Liu,Y.
            and Bolton,D.
  TITLE     Sequencing of the Escherichia coli O2, O28ac, O63 and O42 O antigen
            clusters and detection of pathogenic strains by multiplex PCR
            assays targeting virulence genes in the respective O-antigen gene
            clusters
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8027)
  AUTHORS   Yan,X. and Fratamico,P.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2008) Microbial Food Safety Research Unit,
            USDA-ARS, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="P11a(O42:K-:H37)"
                     /serotype="O42"
                     /db_xref="taxon:562"
     misc_feature    <1..>8027
                     /note="O-antigen gene cluster"
     gene            518..1588
                     /gene="wzx"
     CDS             518..1588
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACK44377.1"
                     /db_xref="GI:217426216"
                     /translation="MSLILTDFGFNLSSTEYIARNCQSKDKINKHIGAVFQIKILLIF
                     LCVIFLYFLVNDQLQQFKIEFFFYSCIAIIGQTFQSLWFFQGVEKMKRITLFLTISKF
                     SYVILTFVIVKKPEQGRFVILAYALSQVIGALLSQYYMRKEGYKYTPANLSSMLGVLR
                     TSALFFWSRLAVSTYTTASTLVVGAAGHVQAGYYAASEQLYKAGQNITTPVSQALYPY
                     MTRAKNWALFFKMMSILTVVMITASLTAIWLSQWLIALIFGPEYIVSQNVFIVFMLAL
                     IVTFVSTNFGYPVFSALGKVKVANYTVIFGALTHVVILGVLFCLGEISAYNVVRAVLF
                     TEIVILILRMFFFVKLYRREVK"
     gene            1585..2763
                     /gene="wbeS"
     CDS             1585..2763
                     /gene="wbeS"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol phosphotransferase"
                     /protein_id="ACK44378.1"
                     /db_xref="GI:217426217"
                     /translation="MIIKKVISKLFGLFFCALDYVWKKQDINIFGAGRGSRYFDNSKT
                     LFEYYISQGDRAYWFYTGKKPDVVGISPDAFVQANSLYGMLIALRAKKAFACYGSLDF
                     WLYRFSRKTVSVQLWHGIPIKKVFFGEKNLTQRKKLQYLFEISAFQYFIVSSKLEQYI
                     ISAQTGMHLDRVKVLGYPRNDNLFKSANEVIIPEVVALNSNFKKVVLFAPTFRNNDYS
                     VFSSFNDDEWNVFFDFLKTENFLCIFRAHAVEFLQNNDDNAKRLSKYSSNVIFLGQDK
                     YQDAQDLMVYSDILISDYSGMIPDYLLLDKPIIRFIFDEQQYDNERGVNFLQKNLPIG
                     SAAYNMPTLIEALRQCVESTEESVAVNAFAKKMYHDNLCGKYSEKIYNYFNSLSQGKV
                     "
     gene            2760..4139
                     /gene="wzy"
     CDS             2760..4139
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ACK44379.1"
                     /db_xref="GI:217426218"
                     /translation="MKQARIKPFSYLSLSFFVLFLIFLNISAVSFDEYLTDLQYSYAY
                     VLFAISLVAVYYAQGESYTSLGFLFNTCLFLFIGGRFIATTFGIDSESVFTLDFMTTY
                     IPTSDEKLEIYTRVVSFQIFLNLGYLFLRKEDSLLNLNKNDNSLPGRLLFLIVIVLFL
                     LCLVEQYSMLSKAMSEGYLARYGDQAESYDAGAAFKYTLLFVFTGLLFATCKKKYIII
                     NFMIYMTLATIIILIGARGAFVSTILMGVWLYGLNKKISLKKLSIIFLSLAGILLIIM
                     NFSARGNYYSNELIDSLLRFLYDQGNTLAVVGYVRTITDYPVFAYISTFLPGSSFFAS
                     MFHGGSDLNSYMLSFPAYANYVANPNMYFAGYGLGWSIISSVEMFSADILFIFCIFSV
                     LIGMFLKFANEQAKRNSFWFGAVACLCAKILFTARADIKTIIPLFMYYAIMYLIISTA
                     MRILRQRYG"
     gene            4132..5142
                     /gene="wbeX"
     CDS             4132..5142
                     /gene="wbeX"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACK44380.1"
                     /db_xref="GI:217426219"
                     /translation="MDNRDVMFSVIVPCYNVVSHINETVASLNKAIDSACTTVEVIFV
                     NDGSTDKTLDVLNEICSNKPSYRVIDQENGGVSNARNTGIRESTGKYILFLDSDDTYK
                     NNIFSILSNISFSDDIVFFSYEKIDQSGNTRVYTIPNSYMAENSITVLKDLFSKKIYL
                     NICALAVNRDCIFQNNIFFDESIQHCEDLQFIIKMITYSKSFQFINEPLFTYNYTPGS
                     AVNSKVDEKHYSKFIAFENMRDVFIKNDKYKELIPAYDFYVATVYLLLLKTIVSNGVV
                     NNKQLSKFVEYSFILKRKMELPPNTMGAFVLIFNIINYIFPMKVKFLMMSFILRMNKG
                     LR"
     gene            5139..6056
                     /gene="wbeY"
     CDS             5139..6056
                     /gene="wbeY"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACK44381.1"
                     /db_xref="GI:217426220"
                     /translation="MSITNKTIALVIVTYNRCNLLMEMLSSIENMSVKPDIVYVIDNN
                     SSDNTSSVVTECDSRKNINIKYHNTGYNAGGAGGFYIGSKMAYEDGWDRIWLADDDIV
                     LDKECLNNAMEYDDGRTILQPMRYNMDGSCAEISAIQYDLSNPFYLRPKRKTVQNIFN
                     KNILSYDIQSIPFEGPIIPREVFNVIGFPDERFFIFNDDLDFAIRAQRAGFSIKCITN
                     AKIVRKIPFVQSVALKTWKGYFMFRNYFRVQKVYGSSPLIYLRILLVFCLALGHSLVR
                     MDINSIKMLCGALKDGLSQEFKLTEKYKP"
     CDS             6053..7123
                     /codon_start=1
                     /gene="wbeZ"
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="ACK44382.1"
                     /db_xref="GI:217426221"
                     /translation="MSTTDSPNIKTSNNNFDIVRLILALVVVFSHIRDLNNINSRFFS
                     IFSGDFAVECFFVISGYLIIRSFKNNQNIRKYIFSRGMRILPMYYVSIVSFFVILFFI
                     SHLSVVEYLKEGGIRYLVFNGVFLNFIQPSLPGVFVDNPRISAVNGALWTIKVEILFY
                     IFVPFFVLFIKKINRHIIPIFIILILSSIVFKWIVTDYHQILHIPEQLVNQLPSVLSY
                     FLLGGLVNYLDLSKISKKTVFWFFIVSAFYLLLSSKTSLDLLVRPFVIAGFILPICLQ
                     RKFLISVPEKMGDLSYGIYVTHFPLVQLLIAVGLYNSFWLGFCLTFTSLFILSFISWH
                     LIEKPALRFAKSAGKNKVVRGV"
     CDS             7120..>8027
                     /codon_start=1
                     /gene="glf"
                     /transl_table=11
                     /product="UDP-galactopyranose"
                     /protein_id="ACK44383.1"
                     /db_xref="GI:217426222"
                     /translation="MRIAIVGAGFSGAVIARELAEHGHSVDIFEKRDHVAGNCYSKRD
                     PDTNVMVHVYGPHIFHTDDKEVWEYINKFGMFEPYVNRVKAITKGAVYSLPINLHTIN
                     QFFHKTLNPAEAKDFINSLCELFEFEPITFEQQALSFIGRDLYEAFFRTYTIKQWGLE
                     PSELPASILKRLPVRFNYNDNYFSHRYQGMPRDGYTNIISAILDHASIAVKLNTDFKK
                     NVGNEYDHVFYSGTIDGYFDYSHGRLPYRTLDFVTERHKGDYQGTAVINYCDDSEKFT
                     RITEHKYFSPWEEHSNTIIYKEYSRT"
BASE COUNT     2429 a   1246 c   1514 g   2838 t
ORIGIN
        1 catgtgaata ttcttgaaag ggctgccagt cttggcgact atctaatagt tggcgtctct
       61 tctgatgagt tgaattattc aaaaaaacaa cgctatccta tttattctca agaagatcga
      121 accaaaatta tttcatcact aaagtttgtc aatgaagttt tcatagaaga atcccttgag
      181 aaaaagctcg agtacattaa aacatataac gcggatattc ttgttatggg ggatgattgg
      241 gctgggagat tcgattgggt gaaaagtgat tgtgaagtta tttatctgcc tcgaacacca
      301 tctatatcga ctactgaaat tatagaagtc gtcaagtcta ttaggtaggg caggtagatt
      361 gaaacttaag cagaatgtat tgtttggtaa tattgcggcg ctggcgatat tacaattagc
      421 tacatatatc acgcctttgt taattgtccc ttatcttact cgagtgttag gtccgggagg
      481 ctatggcact gttgcaattt gtttgtcaat aattcagatg tcactaatat tgactgactt
      541 tggttttaat cttagttcta cagaatatat agcaagaaat tgtcaatcta aagataaaat
      601 aaataaacat attggtgcag tttttcaaat taaaatatta ttgatttttc tgtgtgtgat
      661 ttttctttat tttttagtaa atgaccaatt gcaacaattt aaaatagagt ttttctttta
      721 ttcatgcatt gctattattg ggcaaacatt tcaatcccta tggtttttcc aaggggtaga
      781 aaagatgaag cggattacac tttttttaac tatatcaaag ttttcatatg ttatcctgac
      841 ttttgtaatt gtaaaaaaac cagagcaagg acgatttgtc attcttgctt atgcattatc
      901 tcaagtcatt ggcgcattat tatctcaata ttacatgcgt aaagaaggtt ataaatatac
      961 ccctgcaaat ttatccagca tgttgggggt tttacgtacc agtgcattat tcttttggtc
     1021 ccgacttgct gtctccacat atacaactgc gagcacattg gtcgtaggag ctgcaggaca
     1081 tgtgcaagct ggttattatg ctgccagcga acaactgtat aaggcaggtc aaaatataac
     1141 gaccccagtc agccaggcat tatatcctta tatgacacgg gcaaaaaatt gggccctgtt
     1201 ttttaaaatg atgtctattc ttacggttgt gatgattacc gctagcctaa ctgctatttg
     1261 gttaagtcag tggctgattg ctttaatctt tggtccagaa tatatagtta gccagaatgt
     1321 attcatagtt ttcatgcttg ccctaatagt cacatttgta agtacaaatt ttgggtaccc
     1381 tgtattttcc gcgcttggga aagttaaagt tgcaaattac actgttattt tcggtgcttt
     1441 aacgcatgta gttattctgg gtgttttgtt ttgtttgggt gaaataagtg cctataacgt
     1501 agttagggct gtattattta cagaaatagt catattgata ttgcgaatgt tcttctttgt
     1561 gaagttatac aggcgagaag taaaatgata attaaaaaag ttatatccaa attatttggc
     1621 ttgttttttt gtgcgctgga ttacgtttgg aaaaaacaag atataaatat atttggagca
     1681 ggtagaggct ctcgttattt tgataactcg aaaacactct ttgagtatta tatatctcaa
     1741 ggtgatcgag cctactggtt ctacacagga aagaagccag atgttgttgg aatctctcct
     1801 gatgcatttg tacaggctaa cagtttgtat ggaatgttga ttgcattacg tgcaaaaaaa
     1861 gcattcgcat gttacggttc tttagatttt tggctatata ggttttctcg taaaacagta
     1921 tccgttcaat tatggcatgg catacccatc aaaaaggttt tctttggtga gaaaaattta
     1981 acgcagcgta aaaaacttca gtatttattt gagatttcag cttttcagta tttcatagta
     2041 tcctcaaaac ttgaacagta tattatttca gctcaaaccg ggatgcatct ggatcgagtt
     2101 aaggtcttag gttatcccag gaatgataat ttatttaaat cagcaaatga agtgattatc
     2161 cctgaagtag ttgcattgaa cagtaatttc aaaaaagttg ttttattcgc accaacattt
     2221 aggaataacg attattctgt atttagttca tttaatgatg atgaatggaa tgtttttttc
     2281 gatttcttaa aaacagagaa ttttctttgt atctttcgag cgcatgctgt tgagtttctc
     2341 caaaataatg atgataacgc taaaagattg tctaaatact caagtaatgt catttttctt
     2401 gggcaagaca aatatcagga tgcacaggat ttaatggttt acagcgatat tctgattagt
     2461 gactattccg gtatgattcc agattacttg ctactggata aacctatcat cagatttatt
     2521 tttgatgagc agcagtatga taacgagcgt ggcgttaact ttttgcaaaa aaatctgcct
     2581 ataggctcgg ccgcgtacaa tatgccgaca ttaatagaag cattgagaca atgtgtagaa
     2641 agcacagaag agtcggtggc agtaaatgcg tttgcaaaaa aaatgtatca cgataattta
     2701 tgtgggaaat attcagaaaa aatatataac tattttaatt ctttatcaca ggggaaggta
     2761 tgaaacaagc aagaataaag cctttctcat atttatcatt atcttttttt gtgttgttct
     2821 taattttttt aaatatttca gcagtttcat tcgatgagta tctgacagac cttcaatatt
     2881 catacgccta tgtgctgttt gctatatctc ttgtggcggt ctactacgct caaggtgagt
     2941 cttatacgag tcttggtttc ttgtttaata cctgtttatt tttgttcata ggcggtcgtt
     3001 ttattgcgac aacgtttgga atagacagtg agtcggtgtt tactttagat ttcatgacta
     3061 catatatacc aacatcggat gagaaattag aaatatacac aagagtagta tcatttcaaa
     3121 tatttttaaa tttgggctat ttgtttttac gtaaggaaga tagtttatta aatctgaata
     3181 aaaatgacaa ttcacttccg ggtcgtcttt tatttttaat tgttatagtg ctgtttttat
     3241 tatgccttgt ggaacaatac tcaatgctgt cgaaagctat gtctgagggg tacctggcca
     3301 gatatggcga tcaagccgaa agttatgatg caggggctgc atttaaatat acgctgcttt
     3361 ttgtttttac aggcttatta tttgctacat gtaaaaagaa gtacattatt ataaatttca
     3421 tgatttatat gaccctggct actatcatta tactaattgg tgcacgggga gcatttgtat
     3481 caaccatact aatgggggta tggttatacg gtttaaataa gaaaataagt ttaaagaaac
     3541 tttccattat tttcttgtcg ttggccggaa tacttttaat tatcatgaat ttttctgcac
     3601 gtggcaatta ttattcaaat gaactcatcg attctttatt gcgtttcctc tatgatcagg
     3661 gtaatacact tgctgtggtg ggttatgtaa gaaccattac cgattatcca gtgttcgcat
     3721 atatttcaac atttttgcct ggaagttcat tctttgcatc aatgtttcat ggtggaagtg
     3781 atttaaatag ctacatgcta agttttcccg cttatgcaaa ctatgttgct aatccaaata
     3841 tgtattttgc tggatacggc ctgggatggt caatcatcag ttctgtggaa atgttctccg
     3901 ccgatatctt attcattttt tgcatttttt cggtccttat tggtatgttt ttaaagtttg
     3961 ctaatgaaca ggcaaaaagg aatagtttct ggtttggggc tgttgcgtgt ttgtgtgcga
     4021 aaattttatt tacagctcgt gctgatatca aaacgataat tccactattt atgtattatg
     4081 caataatgta tctaattata agcactgcta tgcgaatttt gaggcaaaga tatggataat
     4141 agggatgtaa tgttttcggt tatagttcca tgctataacg tggtttcgca tataaatgag
     4201 actgtagctt cacttaataa agctatcgat tcggcctgta ccacggtcga agtcattttt
     4261 gttaatgatg gctctacaga taaaacattg gatgttttaa acgaaatctg cagtaacaaa
     4321 ccatcatatc gtgttattga tcaggaaaat ggtggtgtct caaacgcaag gaatacagga
     4381 ataagagagt ctacagggaa atatattctc tttctggatt cagatgatac atacaaaaat
     4441 aatatatttt cgattttatc gaacatatca ttttcagatg atattgtttt ctttagctat
     4501 gaaaagatag accagagcgg taatacgagg gtttatacta ttccaaactc ttatatggcc
     4561 gagaattcaa ttactgtatt aaaagatctt tttagtaaaa aaatctattt aaatatttgt
     4621 gcgctagctg taaataggga ttgtatattt cagaataata tttttttcga tgaaagtatt
     4681 caacattgtg aggatcttca attcatcatt aaaatgatta cttattcaaa atcattccaa
     4741 ttcataaatg aacctttatt tacctataat tacactcccg gttctgctgt taatagcaag
     4801 gtagatgaga aacattattc aaaatttatt gcctttgaga acatgcgcga tgtttttata
     4861 aaaaatgata aatataaaga gttaatcccg gcgtacgatt tttacgttgc aacagtatac
     4921 ttattactac ttaaaacgat tgtctcaaat ggtgtggtta acaacaaaca actaagtaag
     4981 ttcgttgaat attcttttat cctcaagaga aaaatggaat taccaccaaa tacaatgggt
     5041 gcttttgttt tgatatttaa cataattaat tatatttttc ctatgaaagt aaaattttta
     5101 atgatgtcat ttatcttacg gatgaataaa ggtttaagat gagtattaca aataaaacaa
     5161 ttgcgctggt cattgtaaca tataatagat gtaacctttt gatggaaatg ctttcctcta
     5221 tcgaaaacat gagtgttaag cctgatattg tctatgttat agacaataac agtagtgata
     5281 atacttcatc ggttgtcaca gagtgtgatt caagaaaaaa tattaatata aaatatcaca
     5341 acactggata taacgctgga ggtgctggcg gcttttatat cggcagtaaa atggcttatg
     5401 aagatggttg ggatagaatt tggcttgcgg atgatgacat tgttttggat aaggagtgtc
     5461 ttaataacgc aatggagtat gatgatggac gcactatctt acaacctatg cgttataata
     5521 tggacggatc ctgtgccgaa atttcagcta tccagtacga tttaagtaat cctttttatt
     5581 tacgtccaaa acgcaagacg gttcagaata ttttcaacaa aaatattctg tcatatgata
     5641 tccaatccat tccgtttgaa ggaccgatta ttcccaggga agtatttaat gtcataggtt
     5701 ttccagatga acgtttcttt atatttaatg acgatttaga ttttgcgatt agagctcaga
     5761 gagctggctt cagcataaaa tgtattacta acgcaaaaat cgtacgtaaa attccctttg
     5821 tacagtcggt agcattaaaa acatggaagg gctatttcat gtttagaaat tattttcggg
     5881 ttcagaaagt ttatggttca agcccactta tttatctgag aattttgctc gtgttctgcc
     5941 ttgcactggg acattcactc gtgcgtatgg atatcaattc aattaagatg ctgtgcggtg
     6001 cgttgaaaga tggtctctct caagaattta agctgactga gaaatacaaa ccatgagtac
     6061 taccgatagc cctaatataa aaacatcaaa taacaatttt gatatagtaa ggctcattct
     6121 tgcgttagtt gttgttttct cacatataag agatttaaat aatattaatt cccggttctt
     6181 ctccattttc tcaggtgact ttgccgtaga atgctttttc gtaattagcg gttatttgat
     6241 cattagaagc tttaaaaata atcaaaatat cagaaaatat attttttcga gggggatgag
     6301 aattctccct atgtactacg tttctatagt atcctttttc gttatattat tttttatctc
     6361 tcacctttcc gttgttgaat atttaaagga aggcggaatt cgatatttag tatttaatgg
     6421 agtattcctc aatttcatcc agccttcgct gccgggagtg ttcgttgata atcctcgaat
     6481 ctctgcggtt aatggggctc tttggactat aaaagttgag atcctattct atatatttgt
     6541 tccattcttt gtcctgttca ttaaaaaaat caatcgacat attattccta tatttataat
     6601 tcttatttta tcgtctattg tattcaagtg gattgttaca gactatcatc aaattttaca
     6661 tattcctgag caactggtta atcagttacc gtcggtgcta agttattttc tattaggcgg
     6721 cttggttaat tatctggatc tatctaagat atcaaaaaaa acagtatttt ggttttttat
     6781 agtatcagct ttttatcttt tactaagtag taaaacgtca ttggatttac ttgttcgccc
     6841 atttgttatt gcagggttca tactgccgat atgtttgcag cgtaaatttt taataagtgt
     6901 gcctgaaaaa atgggtgacc tttcgtatgg tatatatgta acacatttcc ctcttgttca
     6961 attactaatt gcagtagggt tgtataattc attttggtta ggtttttgtt taacatttac
     7021 tagtcttttt attttgtctt ttattagttg gcatcttatt gagaagccag ctcttcgttt
     7081 tgctaaatct gcaggaaaaa ataaagttgt gaggggtgta tgagaattgc tattgttggt
     7141 gctggattta gtggtgcagt tatagccagg gaattggctg aacatggtca ctcagtagat
     7201 atctttgaga aaagagacca tgttgctggt aattgttata gtaaaagaga cccagacaca
     7261 aatgttatgg ttcatgttta tggcccgcat attttccata cggatgataa agaagtatgg
     7321 gaatatatca ataaattcgg aatgttcgag ccatatgtta atagagtcaa ggccataact
     7381 aaaggcgcgg tttattccct gccaatcaat ctccatacaa ttaaccagtt tttccacaaa
     7441 actctgaacc ctgctgaagc gaaagacttc ataaattctt tgtgtgaact attcgaattt
     7501 gagccgatta cattcgagca gcaggcttta tcatttattg gtcgagactt gtacgaggca
     7561 ttttttagaa cgtataccat taaacaatgg ggtcttgaac cttcagagct tcctgccagt
     7621 attttgaaac ggctaccagt tagatttaat tataacgata actatttcag ccatcgttat
     7681 cagggtatgc cacgtgatgg atatactaat ataattagtg ccattttgga ccatgcaagt
     7741 atcgctgtga aattaaacac tgatttcaaa aaaaatgtcg gcaatgaata tgatcacgtg
     7801 ttttatagcg gcacgattga cggttatttt gactactccc atggccgcct tccttacagg
     7861 actctagatt ttgtcacgga aagacataaa ggtgattatc aaggaaccgc tgtaattaac
     7921 tattgtgatg atagtgagaa atttactcgt attactgaac acaaatattt ttccccttgg
     7981 gaagagcaca gcaatacaat aatctataaa gaatacagcc ggacatg
//
