LOCUS       AB811619               19971 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli DNA, O-antigen biosynthetic locus, strain:
            Bi7455-41.
ACCESSION   AB811619
VERSION     AB811619.1  GI:733166346
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 19971)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..19971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Bi7455-41"
                     /db_xref="taxon:562"
                     /note="serogroup:O43"
     gene            <1..906
                     /gene="wcaM"
     CDS             <1..906
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="BAQ00920.1"
                     /db_xref="GI:733166347"
                     /translation="NYAILRQGFHNQLIGANITNCKFSDLQGDAIEWNVAINDRDILI
                     SDHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVE
                     NGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVI
                     KGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAASFVALTNIEMKRASLELHNK
                     PQHLFMRNIKVMQESSVGAALRMNFDMRKDVRGVFMAKRETLLSLANIHAVNETGQSS
                     VDIDRINHHIVNVEKINFRLPERRE"
     gene            1064..2059
                     /gene="gne"
     CDS             1064..2059
                     /gene="gnu"
                     /note="O043_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAQ00921.1"
                     /db_xref="GI:733166348"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            2302..3195
                     /gene="galF"
     CDS             2302..3195
                     /gene="galF"
                     /note="O043_02"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ00922.1"
                     /db_xref="GI:733166349"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAVLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            3513..4826
                     /gene="wzx"
     CDS             3513..4826
                     /gene="wzx"
                     /note="O043_03"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ00923.1"
                     /db_xref="GI:733166350"
                     /translation="MSLVKIIPKHLWVAASSWGARGISACVQIVSVHYLVSLLGEDKY
                     SAFILLGGLITWCNLSDFGIGNSILNYIAERRAQDKNYEYFILVGSVIVFILFFIVAV
                     FLCFLSMTVAEQYLKIYSDAIINNKIILFYISTLIFCGTALAGIIYKIWYAEQIGWLS
                     NLFPAVCALIGLLNIIVVRKIDGNFSDLLIVFVLFYLPALIIPIYLLLKRYRFEIRKT
                     KLNRRLFAGASLKIIERARPFFIFAVMGAIVLQTDLIVLSQKGDASEIILYGVLLKIF
                     NVIYFIYSAVLQAWWPVCTELRIKKQWVELKKSIRLSVGLGALAITTLIFLVYCLQDV
                     VFKLLGLGGIEKGSFILYCLFCLYFIIRAWCDTYAVLLQTMNSMKPLFIIVPIQALIN
                     FILQWYFISILSLTGILIGSIISFLCTVAIYLPYKFNQQIKKQTH"
     CDS             4839..5585
                     /note="O043_04"
                     /gene="wccT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00924.1"
                     /db_xref="GI:733166351"
                     /translation="MMRKKLSIVTVVYNGERFIENAINSVISQKNEQVEYIIVDGGST
                     DSTLNIIEKYKNKIDIFISEKDKGIYDAMNKGWSKATGEYVAFLNSDDFYQPGLLNSV
                     LNETNKNPDMIVTNTLIQDGEGKRKLFNRVTRKEDYKLHLRLPFMHPSVFIKKCIISK
                     HNGFSLNYKIASDCDLLLKVLNESISIEYVNAYIVMRLGGVSDVNYKLGRKEYRDIYF
                     NHFNRKIKANIGYVESLLSHYCYKAIRILK"
     CDS             5582..6103
                     /note="O043_05"
                     /gene="wccU"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="BAQ00925.1"
                     /db_xref="GI:733166352"
                     /translation="MKSLKIYLWELFFSKIPCNFIRRIFFSKVLNNKINNNISLLRNI
                     HLTSTDGISIDEKTTINKNVYIDGRGGVTIGSNVSISPYVRIITASHDVNCPNFSLIL
                     KPVIIKDYVWICTSSIILPGVTLGYGAIVAAGAVVTKNIPDYAIVAGNPAKVIGYRSE
                     TLHYNPLWRPTFQ"
     CDS             6082..7170
                     /note="O043_06"
                     /gene="wbeX"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00926.1"
                     /db_xref="GI:733166353"
                     /translation="METHVSMRKYYICRDFSEACGISKYGYVFFSTILKEYGFEKIHL
                     KDVNDVSQVLSKINCDDKVWIEIGLGTYLETLLYKKLIRKNNQNVIITLHDAPFIEYP
                     ILKFKNQPFNLLSKINQYVFLRWPFTSFFYSYLHKTKRIYTLNPKGTESLLNRYRLHN
                     VVTIPHVLHNIEEVDNKKSGILQFLYFGFIGKNKGLEYALSLHEKINTLVKTPITMKI
                     IGKTLDEKSEKYFQKIKRRYNENVEYLGYVEDKELDILMQQDNIILLPTKNYKVIKPT
                     SGSVLNSLKYLNIVFTTKVNANECLISDKENGFFLQGDINSDTFNILSIINNSDLRNK
                     IRLNIYNDLMMKYIPIKVINEIKVDDYR"
     gene            7157..8368
                     /gene="wzy"
     CDS             7157..8368
                     /gene="wzy"
                     /note="O043_07"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ00927.1"
                     /db_xref="GI:733166354"
                     /translation="MTIAKKKQLNLINFIRRFSILFFLIIFLSLSPNYIFIFYPELKF
                     VIAILGAILALLGICDVRKIFKTYSSLFLFLFYGVLFFTSIISYIIYGNLNGVYFSLS
                     ILFKFLIVIIFVMSLKDIDILFLIKILINIMAVVCLFSLIGEFLYILNIIAVTNSFEF
                     QTYLYHVISYWGAFTVSFDIYGMKIIRNQSFFQEPGFFAFYIFITMIFISIVQKLYSK
                     NHFFILYGLFFLTMLSTLSLTGIALSAILSVYIFRGVVINSIASIISMSILTYILISD
                     NPYVNKIGSLDERLYGLINGANVFYSNPMVFLLGAGYESEPLFMFDGKFNNLFFEILL
                     YSGIFNLFIYMGFIIYIFRKCYGIKFKYFLVIIYCMTTPLFWSPIMIIFNVVLLRYNG
                     VKQSLLRLNEK"
     CDS             8365..9414
                     /note="O043_08"
                     /gene="wdaP"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00928.1"
                     /db_xref="GI:733166355"
                     /translation="MILLDGIIFSLQMSGGISVYFYELIKHLDSQRKKHEIILYKNNN
                     DLIKNYATNAEFRFSINIERFLRCRNFCSEATIFHSSYYRLPERQFRGKIITTVHDFT
                     DEIYPRNAYAKILSYQKKKTILNSDGIICISDNTKRDLLNFIPEAKGIPIKVIYNGVG
                     DFSYNPDCVRNIASFKQPYALFVGGRKKYKNFNVCVQTLSQYKNINLVIVGGGDLDFQ
                     ELKMLNRYLKGRYYKKGYVDDQQLNNLYTCALFLFYPSQYEGFGIPVLEAMKSGCPVI
                     ATDCSSVREIASGYALLAKEATVEQFSKCINLLLDESFREKMIYNGLHYAKKFTWDKC
                     FSDTVSFYDEVRYLK"
     gene            9411..10529
                     /gene="gmd"
     CDS             9411..10529
                     /gene="gmd"
                     /note="O043_09"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="BAQ00929.1"
                     /db_xref="GI:733166356"
                     /translation="MKVALITGITGQDGSYLAEFLLEKGYEVHGIKRRASSFNTDRVD
                     HIYQDPHISNPKFHLHYGDLTDTSNLTRILSEVRPDEVYNLGAMSHVAVSFECPEYTA
                     DVDAIGTLRLLESIRFLGLENKTRFYQASTSELYGLVQEVPQKETTPFYPRSPYAVAK
                     LYAYWITVNYRESYGMFACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLYLG
                     NLDSLRDWGHAKDYVRMQWMMLQQDKPEDFVIATGKQISVREFVRMSAKEVGLELEFS
                     GKGVNEIATVVHKTSDCAVGVNIGDVIVRVDPRYFRPAEVDTLLGDPTKAKEVLGWEP
                     EITIEKMCSEMVASDLEKAKQHAFLKSHGYDVAVSLER"
     gene            10533..11498
                     /gene="fcl"
     CDS             10533..11498
                     /gene="fcl"
                     /note="O043_10"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthetase"
                     /protein_id="BAQ00930.1"
                     /db_xref="GI:733166357"
                     /translation="MTKKRIYVAGHRGMVGSAICRHLSEFHDVELVVKTHKELDLTNQ
                     QDVETFFEQEKIDEVYLAAAKVGGIHANNTYPAEFIYQNLMIESNIINSAHRAGINKL
                     LFLGSSCIYPKFAEQPMKESSLLTGVLETTNEPYAIAKIAGIKLCESYNRQYGCDYRS
                     VMPTNLYGTNDNFHPNNSHVIPALMRRFHEAKELGLNEVVVWGTGTPKREFLYVDDMA
                     AASVYVMELDDEVYKKNTQPMLSHINVGTGVDCSIREMAETMALVVGYEGKIVFDRTK
                     PDGAPRKLMDVTRLEKLGWKYRYNLKEGLELTYKWFINNLDSFRR"
     gene            11501..11956
                     /gene="gmm"
     CDS             11501..11956
                     /gene="gmm"
                     /note="O043_11"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="BAQ00931.1"
                     /db_xref="GI:733166358"
                     /translation="MNKRLAPELFKSIVEHTPLISIDLIIRNEEGKALLGQRLNRPAQ
                     NYWFVPGGRILKDESFENAFKRVTLEELGVQISINEAKFLGAYEHFYSDNFSGTNFST
                     HYIVLGYEINTMSHQINYPTLQHSTYNWFDIAELLADSSVHQYTKNYFK"
     gene            11953..13371
                     /gene="manC"
     CDS             11953..13371
                     /gene="manC"
                     /note="O043_12"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="BAQ00932.1"
                     /db_xref="GI:733166359"
                     /translation="MKVIYMLLPVVMAGGSGTRLWPLSRTLYPKQFLSLTSRLTMLQE
                     TLRRLEGVEHRPALVICNEVHRFIVAEQMRNEHLANSGILLEPKGRNTAPAVALAALK
                     AVSSGEDPILLVLAADHDIQDQERFISSILAAKEFAEEGKLVTFGIVPTKPETGYGYI
                     KTGENLNEYGFKVSAFVEKPELDVAKKYLEDGGYLWNSGIFMFRASVFIDELNKFRPD
                     ILKICQQALKSSTQDLDFIRIDNDSFSCCPEESIDYAVMEKTTEAVVVPLNAHWSDVG
                     SWSALWEISSKDKSGNAIRGDVLIHDSSDSYLYSQHRLIGVVGVKDLVVVETKDAILV
                     AHKDKVQKVKNIVEQLKVNNRAEYQQHREIFRPWGSHDSIAEGSRFQVKHVVIHPGHK
                     TAKQIHYHRTEHWIVVSGTAKVHYEDEIFLVSENESTYIPIGVPHFIENPGKIPLEII
                     EVRSGVYLDEDDVVRILNDDGY"
     CDS             13425..14183
                     /note="O043_13"
                     /gene="wdaG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00933.1"
                     /db_xref="GI:733166360"
                     /translation="MDVKMKVSIITVTYNSEKTLRNTLESIELQTYNDIEYIIIDGGS
                     TDNTLKIINEVSTRVTRCLSESDKGIYDALNKGINLSTGDIIGFVHSDDVLARPDIIE
                     TIVGRFHETKADVVYGDLVFFEKNQIDIIKRYWRSGPFKRSKLSLGWAPPHPSFYMRR
                     ELYKDDGYFDLSYRIAADYDQMVRVLKRDDIKVSYVPQVFVKMRLGGESTRIDNAISS
                     TKEIVAVMKNHNVNWKFAIIIRKISKLMQLFAHK"
     gene            14208..15578
                     /gene="manB"
     CDS             14208..15578
                     /gene="manB"
                     /note="O043_14"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="BAQ00934.1"
                     /db_xref="GI:733166361"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLSK"
     gene            15742..17148
                     /gene="gnd"
     CDS             15742..17148
                     /gene="gnd"
                     /note="O043_15"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ00935.1"
                     /db_xref="GI:733166362"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENAQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            17396..18562
                     /gene="ugd"
     CDS             17396..18562
                     /gene="ugd"
                     /note="O043_16"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ00936.1"
                     /db_xref="GI:733166363"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMIIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            18652..19689
                     /gene="wzz"
     CDS             18652..19689
                     /gene="wzz"
                     /note="O043_17"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ00937.1"
                     /db_xref="GI:733166364"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQ
                     AAPKVPDLQEALIGRFSSAFSALAETLGNQKEPEKLTIEPSVKNQQLPLTVSYVGQTA
                     EDAQMKLAQYIQQVDDKVNQELEKDLKDNLALGRKNLQDSLRTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQ
                     TRQNLLDIENLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVL
                     GRNALRNYNSK"
     gene            complement(19786..>19971)
                     /gene="hisI"
     CDS             complement(19786..>19971)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="BAQ00938.1"
                     /db_xref="GI:733166365"
                     /translation="TKRIAQKVGEEGVETALAATVHDRFELTNEASDLMYHLLVLLQD
                     QNLDLTTVIENLRKRHQ"
BASE COUNT     6139 a   3491 c   4266 g   6075 t
ORIGIN
        1 aattacgcca tcttacgcca gggatttcat aaccagctta tcggtgccaa catcaccaac
       61 tgtaagttca gcgacttaca aggcgacgcc attgaatgga acgtggcgat taacgaccgt
      121 gatattttga tatctgacca tgtcatcgag cgcatcaact gtaccaacgg caaaatcaac
      181 tggggtatcg gcataggcct tgcgggaagt acctacgata ataactaccc ggaagaccag
      241 gcagtgaaaa actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat
      301 gttgaaaatg gtaaacattt tgttattcgt aatatcaaag cccgcaatat cacgccggat
      361 ttcagtaaga aagcaggtat tgataacgcc acagtcgcta tttacggttg tgacaatttc
      421 gtgattgata atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt
      481 aaaggcaaat atctctcgat accgcaaaat ttccgagtga ataatattca actggataac
      541 acccatcttg cttataaatt gcgcggcatc caaatctccg ccgggaatgc tgcctccttt
      601 gtggcactga ctaacattga gatgaagcgt gcctcgctgg agcttcacaa taaaccgcaa
      661 catcttttta tgcgtaatat caaagtgatg caggaatcct cagttggagc cgcattgcgc
      721 atgaacttcg acatgcgcaa agacgttcgc ggcgtcttta tggcaaagcg tgagacgctg
      781 ctttcacttg cgaatattca tgcagtgaat gaaacggggc aaagctccgt cgatatcgac
      841 aggattaatc accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg
      901 gagtagattt gcgaccattc ataaacttac tggatataat gttctaaaag attataatcc
      961 tcacactaac tgttaaagct ggggctgggc agcattgact actcagggaa ggtactcatc
     1021 tttatcttgt gtttcttacg taacaaccag actaaagtga tttatgaacg ataacgtttt
     1081 gctcatagga gcttccggat tcgtaggaac ccgactactt gaaacggcaa ttgctgactt
     1141 taatatcaag aacctggaca aacagcagag ccacttttat ccagaaatca cacagattgg
     1201 cgatgttcgt gatcaacagg cactcgacca ggcgttagcc ggttttgaca ctgttgtact
     1261 actggcagcg gaacaccgcg atgacgtcag ccctacttct ctctattatg atgtcaacgt
     1321 tcagggtacc cgcaatgtgc tggcggccat ggaaaaaaat ggcgttaaaa atatcatctt
     1381 taccagttcc gttgctgttt atggtttgaa caaacacaac cctgacgaaa accatccaca
     1441 cgaccctttc aaccactacg gcaaaagtaa gtggcaggca gaggaagtgc tgcgtgaatg
     1501 gtataacaaa gcaccaacag aacgttcatt aaccatcatc cgtcctaccg ttatcttcgg
     1561 tgaacgcaac cgcggtaacg tctataactt gctgaaacag atcgctggcg gcaagtttat
     1621 gatggtgggc gcagggacta actataagtc catggcttat gttggaaaca ttgttgagtt
     1681 tatcaagtac aaactgaaga atgttgccgc aggttatgag gtttataatt acgttgataa
     1741 gccagatctg aacatgaacc agttggttgc tgaagttgaa caaagtctga acaaaaagat
     1801 cccttctatg cacttgcctt acccactagg aatgttgggg ggatattgtt ttgatatcct
     1861 gagcaaaatt acgggcaaaa aatacgctgt cagctctgtg cgcgtgaaaa agttctgcgc
     1921 aacaacacag tttgacgcaa cgaaagtgca ttcttcaggt tttgtggcac cgtatacgct
     1981 gtcgcaaggt ctggatcgaa ctctgcagta tgaattcgtc catgccaaaa aagacgacat
     2041 aacgtttgtt tctgagtaat gcagacgagt cagagaaaaa aggaccaggt tggactacga
     2101 ttattcggaa agaggtactg attaaattat cagtatgcgg aattccgagt aatccgagta
     2161 acccagatgc tgatggtgtg tagtgttatc ctaaggttta agtaaagtta tgcggaacta
     2221 agctttggta tatagccata acgtttggct aacctatggc aataatttac gcactatcag
     2281 ccacttagag gtaggaaaag aatgacgaat ttaaaagcag ttataccggt agcaggtctt
     2341 gggatgcata tgttgcctgc cactaaggcg attcccaaag agatgctacc gatcgtcgac
     2401 aagccaatga ttcagtacat tgttgacgag attgtggctg cagggatcaa agaaatcctc
     2461 ctggtaactc acgcgtccaa gaacgcggtc gaaaaccact tcgacacctc atatgaatta
     2521 gaatctctcc ttgagcagcg cgtgaagcgt caactactgg cggaagtgca atctatctgt
     2581 ccgccgggcg tgaccattat gaacgtgcgt cagggtgaac ctttaggttt aggccactcc
     2641 attttatgtg cacgacccgc cattggcgac aacccatttg tcgtggtgct gccagacgtt
     2701 gtgatcgacg atgccagcgc cgacccgctg cgctacaacc ttgcagccat gattgcgcgc
     2761 ttcaacgaaa cgggacgcag ccaggtactg gcaaaacgta tgccgggtga cctctctgaa
     2821 tactccgtca tccagaccaa agaaccgctg gaccgtgaag gcaaagtcag ccgcattgtt
     2881 gaatttatcg aaaaaccgga tcagccgcag acgctggact cagacatcat ggccgtaggt
     2941 cgttatgtgc tttctgccga tatttggccg gaacttgaac gcacgcagcc aggggcatgg
     3001 ggacgtattc agctgacaga tgccatcgct gaactggcga aaaaacaatc tgttgatgca
     3061 gtgctgatga ctggtgacag ctacgactgc ggtaaaaaaa tgggctatat gcaggcgttt
     3121 gtgaagtatg gcttacgcaa cctgaaagaa ggggcgaagt tccgtaaagg gattgagaag
     3181 ctgttaagcg aataatgaaa atctgaccgg atgtaacggt tgataagaaa attataacgg
     3241 cggtgaagat tcgtggcgaa agtaatttgt tgcgagtgtt cctgccattg ttttatataa
     3301 acaatcagaa taacaacgag ttagcaatag gtttttagtc aaagttttcc aggattttcc
     3361 ttgtttctag agcggattgg taagacaata aaaacctgtc agtttagtta tttttacagg
     3421 aataacttta gatatcgttg gtatatatgc agtatggtgg tagctgtaga gccaagggcg
     3481 gtagcgtgga taatttaaag gattaggggt gaatgagctt agttaaaata attccaaaac
     3541 atttatgggt cgccgcttca tcttggggag ctagagggat tagcgcttgt gtacaaattg
     3601 taagtgtgca ttatttagtc tctttactcg gagaggataa atattctgct tttattttac
     3661 tgggggggtt aattacatgg tgtaatttat ctgacttcgg tatcggaaat agtatattaa
     3721 attatatcgc agaaagaagg gcacaagaca aaaattacga atattttata ttagtaggat
     3781 ctgttatcgt ttttatatta ttttttattg tagcagtgtt tctttgtttt ttatcaatga
     3841 cagtggcaga acaatatcta aaaatatact cagatgctat tattaataat aaaattattt
     3901 tgttttatat atcaacattg attttttgtg gtactgcgct tgctgggatt atttacaaaa
     3961 tatggtatgc tgagcaaatt ggttggttat caaacttatt tccggcagtc tgcgcattaa
     4021 ttgggctttt aaatattatt gttgttcgta aaattgacgg taactttagt gatttgctga
     4081 ttgtttttgt tttattttat ttgccggctt tgattatacc tatttatctt ttgttgaaaa
     4141 gatatcgatt tgaaataaga aaaacaaaat tgaatcgaag gctttttgct ggtgcaagtc
     4201 ttaagattat tgaaagggct cgaccatttt ttatttttgc agtcatgggg gcgattgtcc
     4261 ttcaaactga tttaatagta ctatctcaaa aaggagatgc aagtgaaatc atattatatg
     4321 gagtgttatt aaagatattt aatgtgatat attttattta tagcgcagtt ctccaggcgt
     4381 ggtggccagt ttgtaccgaa ttacgcataa aaaaacaatg ggtggaactt aaaaaaagta
     4441 taaggctatc tgtagggtta ggtgcattag caataaccac gttaattttt cttgtttatt
     4501 gtttacaaga tgttgtattt aaattgctag gtttaggggg aatagaaaaa ggttcattta
     4561 tattatattg tttgttttgt ttgtatttta tcattcgtgc atggtgtgat acctatgcag
     4621 ttttgttaca aacaatgaat agtatgaagc cgctgtttat tattgtaccg atccaagcgt
     4681 tgattaattt tatattgcag tggtatttta tatcaattct atcactgaca ggtatattaa
     4741 ttggttcaat aatatcattc ttgtgtactg tcgcaatata tttaccctac aaattcaatc
     4801 aacaaataaa aaaacaaaca cactgataga gggagtggtt gatgcggaaa aaattatcaa
     4861 tagtgactgt cgtatataat ggggagaggt tcattgaaaa tgcaatcaat agtgttatat
     4921 cccaaaaaaa tgagcaagtt gaatatatta tagtagatgg ggggtctaca gattcaacgt
     4981 taaatattat agaaaaatat aaaaataaaa tagatatttt tatttcagaa aaagataaag
     5041 gtatatatga tgcaatgaat aaaggatgga gcaaagctac tggagaatat gtggctttct
     5101 tgaatagtga tgatttttat cagcctggat tgctaaattc cgttttaaat gaaactaata
     5161 aaaatccgga tatgatagta actaatacgt tgattcagga tggcgaggga aaaagaaaat
     5221 tatttaatag agtgactaga aaagaagatt ataaattaca tttacgactg ccgtttatgc
     5281 atccatccgt gtttataaaa aaatgtatta tatccaaaca taatggattt agtttaaatt
     5341 ataaaatagc atcagattgt gatcttttgc ttaaagtgct aaacgaatca atttcaattg
     5401 aatatgtcaa tgcttatatt gttatgcggt taggtggtgt aagtgatgtc aattataaat
     5461 tgggcagaaa agaatataga gatatatatt ttaatcattt taatagaaaa ataaaagcta
     5521 atataggtta tgttgagagt ttattatcgc attattgtta taaagctata aggattctaa
     5581 aatgaaatcg ttaaagattt atttatggga gttgtttttt tcaaaaatac catgtaattt
     5641 tattcggcga atatttttct ctaaggtttt aaataacaaa attaataata atatatcatt
     5701 attgagaaat attcatctaa cttcaactga tggtatatca attgatgaaa aaacgaccat
     5761 aaataaaaat gtttatatcg atggaagagg aggggtgaca attggttcta atgttagtat
     5821 ctccccttat gtcaggatta ttaccgcgag tcacgatgta aattgtccta atttttcttt
     5881 aatactgaag cctgtgataa ttaaagatta tgtctggata tgcacatctt ctattatttt
     5941 accaggcgta accttgggct atggagccat tgtcgcagca ggagcagttg ttacaaaaaa
     6001 tattcctgat tatgctattg tcgctggcaa ccctgcgaag gtaattgggt atcgtagtga
     6061 aaccctgcac tataatccat tatggagacc cacgtttcaa tgagaaaata ttatatttgt
     6121 agagattttt ccgaagcctg tggaatttca aaatatggat atgtattctt tagtacaata
     6181 ctaaaagaat atggtttcga aaagatccat ttaaaagatg taaatgatgt tagtcaagta
     6241 ttatcgaaga tcaattgtga tgataaagtt tggattgaaa ttgggttggg aacatattta
     6301 gaaactctat tgtacaagaa attaattagg aaaaataatc aaaatgtaat aattacatta
     6361 catgatgcac cgtttattga atatcctatt ttaaaattta aaaatcaacc tttcaattta
     6421 ttatccaaaa taaatcaata tgtttttttg agatggccat ttacgtcatt tttttatagt
     6481 tatttacata aaacaaagcg tatatataca ctaaacccta aaggtacaga atcacttctt
     6541 aaccgttatc gtttgcataa tgtagttact attccacatg ttttacataa tatagaagaa
     6601 gtagataata agaaaagtgg tatactacag ttcttatact ttggttttat aggtaaaaat
     6661 aaaggattag aatatgcgct cagtttacat gagaaaataa atacattagt taaaaccccc
     6721 attacaatga aaattattgg aaaaacacta gatgaaaaat ctgagaagta ctttcaaaaa
     6781 ataaaaagaa gatataatga gaatgttgaa tatttagggt atgttgaaga caaagaactt
     6841 gatatattaa tgcaacaaga taatattatt cttttaccaa caaaaaatta taaagttata
     6901 aaacctacca gtggaagtgt tcttaattcg ttaaaatatc ttaatattgt atttacaaca
     6961 aaagttaatg ctaatgaatg tctaataagt gataaagaaa atggcttttt tttacaagga
     7021 gatataaata gcgatacatt taatatttta agcattatta acaattctga tttaagaaat
     7081 aaaataagat taaatatata taatgatttg atgatgaagt atataccaat caaagttatt
     7141 aatgagatca aagttgatga ctatcgctaa aaaaaaacaa ttgaacttaa taaattttat
     7201 tcgcaggttc tctatactgt tttttttaat tattttttta tctttatctc cgaattacat
     7261 atttattttt tatcctgaac taaaatttgt aattgctatt ttgggtgcaa tacttgcatt
     7321 acttggcata tgtgatgtta gaaaaatatt taaaacatat tcgtctctat tcttattcct
     7381 tttttatgga gtcctttttt ttacttcgat aattagttat attatatatg gtaacttgaa
     7441 tggagtgtat ttttccttat ctattttatt taagtttctg atagttatca tattcgtaat
     7501 gtctttaaaa gatattgata ttctatttct gataaagata ttaataaata ttatggctgt
     7561 tgtatgtttg ttttctttaa ttggagagtt tttatacatt ctaaatatta tagcagtaac
     7621 aaacagtttt gaatttcaaa catatttata tcatgttatt tcttattggg gagcttttac
     7681 agtttcattt gatatatatg gcatgaagat aattagaaat caatcttttt tccaagagcc
     7741 gggttttttt gcattttaca tttttatcac aatgattttt atttcaatag tgcagaagct
     7801 atatagcaaa aaccattttt tcatactata tggtttgttc ttcttgacta tgctatctac
     7861 gctttctctt acaggaattg cgttatcagc catattaagt gtgtacatat ttaggggggt
     7921 tgttattaat tcaatcgcaa gcattatcag catgtcaatt ttgacttaca tattgatctc
     7981 tgataacccc tatgttaata aaataggttc tttagacgaa cgtttgtacg gattaattaa
     8041 tggggctaat gtattttatt caaaccccat ggtttttctt ttaggtgccg gttatgagtc
     8101 ggaaccgcta tttatgtttg atgggaaatt caataattta tttttcgaaa tactattata
     8161 ctctggtatt tttaatttgt ttatttatat gggttttatt atttatatct tcaggaaatg
     8221 ttatgggatt aagtttaaat attttcttgt cattatatat tgtatgacaa caccactatt
     8281 ttggtcgcct attatgataa tttttaacgt tgttttactt cgctacaatg gggtaaagca
     8341 gagtctgtta aggttaaatg aaaaatgatt ttattagatg gaataatttt ttcgttgcaa
     8401 atgtcgggtg gaatatcagt ttatttttat gagctaatta aacatctcga tagccaaaga
     8461 aaaaagcatg aaatcatttt gtataaaaat aataatgatt taattaaaaa ttatgcgacg
     8521 aatgcagagt ttcgattttc tataaatata gaaagatttc tgagatgtcg taatttttgt
     8581 tcagaagcaa caatatttca ttcaagttat tatcgtttgc cagagaggca gtttagagga
     8641 aaaataataa ctacagttca tgattttacg gatgagatat atcccagaaa tgcttatgct
     8701 aagatactta gttaccaaaa aaagaaaact attttaaata gcgatggaat tatttgcatc
     8761 tctgataata ccaaaaggga tctattgaat tttattcctg aagcaaaagg aattcctata
     8821 aaagttatat ataatggagt tggagatttc tcttataatc cagattgtgt aagaaacatt
     8881 gcttcattta aacagccata tgcattattc gttggaggac gaaaaaaata caagaacttt
     8941 aatgtctgtg tccaaacact cagtcaatat aaaaatatta atcttgtgat tgttggtgga
     9001 ggtgatctgg attttcaaga attaaaaatg ttaaatagat atttaaaagg acgttattat
     9061 aaaaaaggtt atgtagatga ccagcaatta aataatcttt acacctgcgc tttattttta
     9121 ttttatccat cccaatatga agggtttggt attcctgtgt tagaagcaat gaagtctggg
     9181 tgtcctgtaa ttgcaactga ttgttcttct gtaagagaga tagcatcagg atatgctctg
     9241 ctagccaaag aggcaactgt tgaacaattt tctaagtgta ttaatttatt attagatgaa
     9301 agttttagag agaaaatgat atataatggt ctacattatg ccaagaaatt cacttgggat
     9361 aaatgctttt ctgatactgt tagtttttat gatgaggtga gatatttaaa atgaaagtag
     9421 ctctgattac gggtattact ggacaagacg gttcttattt ggctgaattt ttattagaaa
     9481 agggatacga agttcacggt atcaaacgtc gagcatcttc gttcaacact gatcgagttg
     9541 atcatattta tcaggatcca catattagta atccaaaatt ccatcttcat tatggtgatc
     9601 taactgatac ttctaactta acacgtatac tttcagaagt taggccagat gaagtatata
     9661 atttaggagc tatgagtcat gttgcagttt cctttgaatg tccagaatat accgctgatg
     9721 ttgatgcaat tggaacgtta agattgttgg agtctattcg ttttttagga ttagaaaata
     9781 agacaagatt ttatcaagca tcaacatctg agttgtatgg attggttcaa gaagttccac
     9841 aaaaagaaac aacgcctttt tatccaagat caccatatgc agttgccaag ctttatgcct
     9901 attggataac tgttaactat cgtgaatcat atggtatgtt tgcttgtaat ggcattttgt
     9961 tcaatcatga atccccacgc cgtggagaaa cattcgtaac gcgtaaaatt acacgtgcta
    10021 tagctaacat ttctcaaggt attgaaaaat gcctatatct cggtaatctg gattcgcttc
    10081 gtgattgggg acatgctaaa gattatgttc gtatgcaatg gatgatgctc caacaagaca
    10141 aaccagagga tttcgttata gcaacaggta aacaaatatc tgtgcgtgaa tttgttcgta
    10201 tgtccgctaa agaagttggg ctggaacttg aattttcagg taaaggtgtt aatgaaattg
    10261 cgacggttgt acataaaaca tctgactgtg ctgttggggt aaacattgga gatgttattg
    10321 ttcgcgtaga ccctcgctat ttccgtcccg cagaggttga tacgctgttg ggggatccaa
    10381 ctaaagcgaa agaagtgtta ggatgggaac cagaaattac tattgaaaag atgtgttcag
    10441 aaatggttgc tagtgattta gaaaaagcaa aacagcatgc atttcttaaa agccatggtt
    10501 atgacgtagc agtatctctg gagcgttgag taatgacaaa gaagcgtatt tatgttgcgg
    10561 ggcatcgtgg tatggttggt tctgctatat gccgacactt atcagaattt catgatgtcg
    10621 agttagttgt aaaaactcat aaagaactcg atttgactaa tcaacaagat gtcgagactt
    10681 tttttgaaca agagaaaata gatgaagtat atcttgccgc tgcaaaagta ggtggaattc
    10741 acgctaataa tacatatccg gcagaattta tttatcagaa tcttatgata gaaagtaata
    10801 taatcaattc ggctcataga gcaggtatta ataaattact ttttttaggt tcaagttgta
    10861 tttatcctaa gtttgcagag cagccaatga aagaatcttc acttttaaca ggtgtactcg
    10921 aaactacaaa tgagccatat gcaatagcta aaattgcagg tataaaattg tgtgagtctt
    10981 acaatcgtca atatggatgt gattatcgca gtgtaatgcc tactaatttg tatggtacga
    11041 atgataattt tcatcctaat aactcacatg taattccagc gctcatgagg cgttttcatg
    11101 aagcaaagga gttggggtta aatgaagtag ttgtctgggg gactggaaca ccaaaacgtg
    11161 agtttttata tgttgatgat atggcagctg cgtcagtata tgtgatggag cttgatgatg
    11221 aagtttacaa aaaaaatacg caaccaatgt tatctcatat taacgtaggt actggtgttg
    11281 attgttctat acgcgaaatg gcagaaacaa tggctttagt tgttggctat gaaggaaaaa
    11341 ttgtttttga tagaactaaa ccggatggcg caccgcgtaa acttatggat gttactcgtc
    11401 ttgaaaaact gggctggaaa tatcgttata atttaaagga aggactagaa ttaacttata
    11461 agtggtttat aaataacctt gactcattta ggagataata ttgaataaga gattagcacc
    11521 tgagttattt aaatcaatag tcgaacatac tccactaatc tcgatagatc ttattataag
    11581 aaacgaagaa ggtaaggcgc tgcttggcca gcgcctcaat cgtccagcac aaaattattg
    11641 gtttgtgccg ggggggcgaa ttcttaaaga tgagtctttc gagaacgcat tcaaaagagt
    11701 tacacttgaa gaactaggtg ttcaaattag tattaatgaa gcaaaatttc ttggtgcata
    11761 tgaacatttc tacagcgaca acttttcagg aactaatttt tcaacgcatt atatagtact
    11821 tgggtatgag attaatacga tgtcacatca aattaactat ccaacactgc aacatagcac
    11881 ttacaattgg ttcgatatag ccgaattgtt agctgattct tcagttcatc agtacacaaa
    11941 aaattatttt aagtgaaggt tatttatatg ctacttcctg ttgtaatggc cggtggttct
    12001 ggaactagat tgtggccact ttctcgaaca ctatatccga aacaattcct atcgttaact
    12061 agtcgtttga ctatgctaca agagacctta agaaggcttg agggggttga acatcgtcct
    12121 gctctggtta tttgtaatga agttcatcgt tttatcgttg cagaacagat gcgtaatgaa
    12181 catttagcga atagtggcat tttacttgag ccgaaaggtc gtaatacagc tcctgcagtt
    12241 gctcttgctg ctcttaaagc agtttcatca ggcgaggatc ctatactgct tgtattagca
    12301 gccgatcatg atatacaaga ccaagagcgc tttatatcgt cgatattggc tgcaaaagaa
    12361 tttgcagagg aaggtaagct ggttactttt ggtattgtgc caaccaaacc cgaaactgga
    12421 tatggctaca taaaaaccgg agagaattta aatgaatatg gctttaaagt ttctgctttt
    12481 gttgaaaaac ctgagctaga tgtggccaaa aaatatctcg aagatggagg ttatctttgg
    12541 aatagtggaa tattcatgtt cagggcctct gtgtttattg atgagttaaa taaatttaga
    12601 ccagacatac taaaaatatg ccaacaagca ttgaagtcct ctacacaaga tcttgatttt
    12661 atccgtatag ataatgactc atttagttgt tgccctgaag aatctattga ttatgctgtt
    12721 atggagaaaa caacagaggc tgtggtagtt ccattaaatg cccactggag cgatgttggt
    12781 tcttggtctg cattatggga aattagcagc aaagataaaa gtggaaatgc aattagagga
    12841 gatgtattaa ttcatgattc ttcggatagt tatctttact cacaacatag attaattggt
    12901 gtagtaggag ttaaagattt agtagttgtt gaaactaaag atgctattct cgttgcgcat
    12961 aaagataaag ttcagaaggt aaaaaatatt gttgaacaac ttaaagttaa taaccgtgcg
    13021 gaatatcagc agcatcgtga aatatttaga ccatggggca gtcatgactc tatagcagaa
    13081 gggtctcgat ttcaggttaa acatgttgtt attcatcctg gacataaaac tgctaaacag
    13141 atccattacc atcgtactga acattggatt gttgtttccg gaacagcaaa agtccattat
    13201 gaggatgaaa tatttcttgt ttctgaaaat gaatcaactt atataccaat aggtgtaccg
    13261 cattttattg agaatcctgg aaagatccct ttggaaatta ttgaagtacg ttcaggtgtt
    13321 tacttggatg aagatgatgt tgttagaatc ttaaatgatg atggatatta atgcaacatt
    13381 ataattcgta atgctgtact gacgttattt aattatgatt attattggat gttaaaatga
    13441 aagtcagtat tattacagtg acttataata gtgaaaagac tttaaggaat actttagagt
    13501 caatagagct gcaaacctat aacgatatag agtatataat tattgatggt ggttctaccg
    13561 ataatacgtt aaaaataatt aacgaagtat caacaagagt aacaagatgt ctatcagaaa
    13621 gcgataaagg tatctatgat gctttaaata aaggtataaa tttatctacg ggagatatta
    13681 tcgggttcgt tcattctgat gacgttttag caagacctga tattattgaa actattgtag
    13741 gccgtttcca tgagacaaaa gcggatgttg tatacggtga tttggttttt ttcgaaaaaa
    13801 accaaattga tataatcaaa cgatactggc gtagtggccc ttttaaacgt tctaagctat
    13861 cattaggttg ggcaccaccg cacccatctt tctatatgag aagggaatta tataaagacg
    13921 atggatattt tgatttatcc tataggattg ctgcagacta tgatcagatg gtcagggttt
    13981 tgaaacgtga tgatattaaa gttagttatg ttcctcaagt atttgtaaag atgagattgg
    14041 gaggggaaag taccagaatt gataacgcga tatcaagtac aaaggaaatt gtagcagtta
    14101 tgaaaaacca taatgtaaat tggaaatttg ctatcattat cagaaaaata tcaaaactaa
    14161 tgcaattgtt tgcgcataaa taattttata attggtaagg tttttatatg aaaaaattaa
    14221 cctgctttaa agcctatgat attcgcggga aattaggcga agaactgaat gaagatattg
    14281 cctggcgcat tggtcgcgcc tatggcgaat ttctcaaacc gaaaaccatt gtgttaggcg
    14341 gtgatgtccg cctcaccagc gaaaccttaa aactggcgct ggcgaaaggt ttacaggatg
    14401 cgggcgtcga tgtgctggat attggcatgt ccggcactga agagatctat ttcgccacgt
    14461 tccatctcgg cgtggatggc ggcattgaag ttaccgccag ccataatccg atggattaca
    14521 atggcatgaa gctggtgcgc gaaggggctc gcccgatcag cggtgatacc ggactgcgcg
    14581 acgtccagcg cctggcagaa gccaacgact ttcctcccgt cgatgaaacc aaacgcggtc
    14641 gctatcagca aatcaatctg cgtgacgctt acgttgatca cctgttcggt tatatcaatg
    14701 tcaaaaatct cacgccgctc aagctggtga ttaactccgg gaacggcgca gcgggtccgg
    14761 tggtggacgc catcgaagcc cgctttaaag ccctcggcgc gcccgtggaa ttaatcaaag
    14821 tgcacaacac gccggacggt aatttcccca acggtattcc taacccgcta ctgccagaat
    14881 gccgcgacga cacccgcaat gcggtcatta aacacggcgc ggatatgggc attgcttttg
    14941 atggcgattt tgaccgctgc ttcctgtttg acgaaaaagg gcagtttatt gagggctact
    15001 acattgtcgg cctgctggca gaagcgttcc tcgaaaaaaa tcctggcgcg aagatcatcc
    15061 acgatccacg tctctcctgg aacaccgttg atgtggtgac tgccgcaggt ggtacgccgg
    15121 taatgtcgaa aaccggacac gcctttatta aagaacgtat gcgcaaggaa gacgccatct
    15181 acggtggcga aatgagcgcc caccactatt tccgtgattt cgcctactgc gacagcggca
    15241 tgatcccgtg gctgctggtg gctgaactgg tgtgtctgaa aggaaaaacg ctgggcgaac
    15301 tggtgcgcga ccggatggca gcatttccgg caagcggtga gatcaacagc aaactggcgc
    15361 aacccgttga ggcgattaac cgcgtcgaac agcattttag ccgtgaggcg ctggcggtgg
    15421 atcgcaccga tggcatcagc atgacctttg ccgactggcg ctttaacctg cgctcctcca
    15481 acaccgaacc ggtggtgcgg ttgaatgtgg aatcgcgcgg tgatgtgccg ctgatggaag
    15541 aaaaaacaaa acttatcctt gagttactga gtaagtaatc cagtaatttc atataaatag
    15601 gttttaaaag acggaaaaga tgagatatac agtgtggtat agccaaggta atgctattca
    15661 ccatctctat tagtgagtta acatctatac cacatttaag ccgcacactc ggcggtgagc
    15721 accccctgac aggagtaaac aatgtcaaag caacagatcg gcgtcgtcgg tatggcagtg
    15781 atggggcgca accttgcgct caacatcgaa agccgtggtt ataccgtctc tattttcaac
    15841 cgttcccgtg aaaagacgga agaagttatt gccgaaaatc caggcaagaa actggttcct
    15901 tactatacgg tgaaagagtt tgttgaatct ctggaaacgc ctcgtcgcat cctgttaatg
    15961 gtgaaagcag gtgcaggcac ggatgctgct attgattccc tcaagccata cctcgataaa
    16021 ggtgacatca tcattgatgg tggtaacacc ttcttccagg acaccattcg tcgtaaccgt
    16081 gagctttctg cagaaggctt taactttatc ggtactggtg tttccggtgg tgaagaaggt
    16141 gcgctgaaag gtccttccat tatgcctggc ggacagaaag aagcctatga actggttgca
    16201 ccgatcctga ccaaaatcgc cgcagtggct gaagacgggg agccatgcgt tacctatatt
    16261 ggtgccgatg gcgcaggtca ctatgttaag atggttcaca acggtattga atacggtgat
    16321 atgcagctga ttgctgaagc ctattctctg cttaaaggcg gcctgaatct ctctaacgaa
    16381 gaactggcac agacctttac cgagtggaat aacggtgaac tgagcagcta cctgatcgac
    16441 atcaccaaag acatcttcac taaaaaagat gaagacggta actacctggt tgatgtgatc
    16501 ctggatgaag cggctaacaa aggtaccggt aaatggacca gccagagcgc gctggatctc
    16561 ggagaaccgc tgtcgctgat taccgagtct gtgtttgcac gttatatctc ttctctgaaa
    16621 gatcagcgcg ttgccgcatc taaagttctc tctggtccgc aagcgcagcc agctggcgac
    16681 aaggctgagt tcatcgaaaa agttcgtcgt gcgctgtatc tgggcaaaat cgtttcttac
    16741 gctcagggct tctctcagct gcgtgctgcg tctgaagagt acaactggga tctgaactac
    16801 ggcgaaatcg cgaagatttt ccgtgctggc tgcatcatcc gtgcgcagtt cctgcagaaa
    16861 atcaccgatg catatgccga aaatgcgcag atcgctaacc tgctgctggc cccgtacttc
    16921 aagcaaattg ccgatgacta ccagcaggcg ctgcgcgatg tcgtcgctta tgcagtacag
    16981 aacggtatcc cggttccgac cttcgccgct gcggttgcct attacgatag ctaccgtgcc
    17041 gctgttctgc ctgcgaacct gatccaggca cagcgtgact atttcggggc gcatacttat
    17101 aagcgcattg ataaagaagg tgtgttccat actgaatggc tggattaatc ttatttaact
    17161 aacaataaat tgaggcccgg ggtatattgc gccgggcttt tttttgccaa ttatcttata
    17221 gactaaattt tactgcttaa tattaactta ataaatatca gctattctta taaagaaaat
    17281 ctgaattgtt tttcgttgcg ttgaccatcg aacaacgtag cgttaaaact tttagctctt
    17341 atcaggatgc taaaaacatc atgattcaca gttaagttaa ttctgagagc atgaaatgaa
    17401 aatcaccatt tccggtactg gctatgtagg cttgtcaaac gggcttctaa tcgcacaaaa
    17461 tcatgaggtt gtggcattag atattttacc gtcacgcgtt gctatgctga atgatcggat
    17521 atctcctata gttgataagg aaattcagca gtttttgcaa tcagataaaa tacactttaa
    17581 tgccacatta gataaaaatg aagcctaccg ggatgctgat tatgtcatta tcgccactcc
    17641 aaccgactat gatcctaaaa ctaattattt caatacatcg agtgtagaat cagtaattaa
    17701 agacgttgta gagataaatc cttatgcggt tatgatcatc aaatcaacgg tacccgttgg
    17761 ctttaccgca gcgatgcata agaaatatcg tactgaaaat attattttct ctccggaatt
    17821 tctccgtgag ggtaaagccc tttacgataa tctccatcct tcacgtattg tcatcggtga
    17881 gcgttcagaa cgcgcagaac gttttgctgc gttattacag gaaggagcga ttaagcaaaa
    17941 tatcccgacc ctgtttaccg actccactga agcagaagcg attaaacttt ttgcaaacac
    18001 ctacctggcg atgcgcgtgg cgtactttaa cgaactggat agctacgcag aaagtttagg
    18061 tctgaattcc cgtcaaataa tcgaaggcgt ttgtctcgac ccacgaattg gcaaccatta
    18121 caacaatccg tcgtttggtt atggtggtta ttgtctgccg aaagatacca agcagttact
    18181 ggcgaactac cagtctgtgc cgaataacct gatttcggca attgtcgatg ctaaccgcac
    18241 acgtaaagac tttattgccg atgccatttt gtcacgcaaa ccgcaagtgg tgggtattta
    18301 tcgtctgatt atgaagagcg ggtcagataa cttccgtgcg tcttccattc aggggattat
    18361 gaaacgtatc aaggcgaaag gcgttgaagt gatcatctac gaaccggtga tgaaagaaga
    18421 ttcattcttc aactctcgcc tggaacgtga tctcgccacc ttcaaacaac aagccgacgt
    18481 cattatctcc aaccgtatgg cagaagagct taaggatgtg gcagacaaag tctacacccg
    18541 cgatcttttt ggcagcgact aacatcctgt tatcagggcg attttcgccc tgattctctt
    18601 atgttccctt tgtaataatt cattattttt atcatttatc ctatagcatt catggcgatt
    18661 atcgctaaac tatggcgact taaaattatt tcgtccgtta gggtaatgat gagagtagaa
    18721 aataataatg tttctgggca aaaccatgac ccggaacaga ttgatttgat tgatttactg
    18781 gtgcagttgt ggcgcggcaa gatgacaatt atcatctctg tcattgttgc tattgccctg
    18841 gctattgggt atttggcggt agcgaaggag aaatggacgt caacagcaat tatcactcag
    18901 cctgacgtgg ggcaaattgc tggctataac aatgccatga atgttatcta tggtcaggct
    18961 gcacctaaag tgccggattt acaggaggcg ttaattggtc gcttcagttc tgccttctct
    19021 gcattagcag aaacgctggg taatcagaaa gaaccagaaa aacttaccat cgaaccttct
    19081 gtaaagaacc agcaattacc attgactgtt tcttatgttg ggcaaactgc agaggacgca
    19141 caaatgaaat tggcccaata cattcagcaa gttgatgata aagtaaatca agagctagaa
    19201 aaggatctca aggacaacct tgctttggga cggaaaaact tgcaggactc tttaagaact
    19261 caggaagtgg ttgcgcagga gcagaaagat ctgcgtatcc gtcagattca ggaagcattg
    19321 cagtatgcga atcaggcgca ggtgacaaaa ccgcagattc aacagactgg cgaagatatc
    19381 acacaagata cgttgttcct tttggggagc gaagcgctgg agtcgatgat taagcatgag
    19441 gcgactcgtc cgttggtgtt ttcgccaaac tactatcaga ctcgtcaaaa cctgctggat
    19501 atcgaaaact taaaggttga cgatcttgat attcatgctt accgttatgt gatgaaaccg
    19561 acgttaccta ttcgtcgcga tagcccgaaa aaggcaatta ccttgattct ggcggtgctg
    19621 ctgggcggca tggttggcgc ggggattgtg ttggggcgta atgctctgcg taattacaac
    19681 tcgaagtaat cttttcggtt ttaaagaaaa tgggcagggt ggtgacacct tgcccgtttt
    19741 ttttgccgga tgcgacgaca atatcgcatc cgcttacccc gcaactcact gatgccgttt
    19801 acgcaggttc tcaatcaccg tcgttaagtc cagattctga tcctgcaaca gcaccagcag
    19861 gtgatacatc aagtcagatg cttcgttggt cagttcaaag cggtcatgca ccgttgctgc
    19921 cagtgcggtt tccacccctt cttcgcccac tttctgcgca atgcgtttgg t
//
