LOCUS       AY771223               14483 bp    DNA     linear   BCT 08-AUG-2005
DEFINITION  Escherichia coli O45 O antigen gene cluster, complete sequence.
ACCESSION   AY771223
VERSION     AY771223.1  GI:59803190
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14483)
  AUTHORS   DebRoy,C., Fratamico,P.M., Roberts,E., Davis,M.A. and Liu,Y.
  TITLE     Development of PCR assays targeting genes in O-antigen gene
            clusters for detection and identification of Escherichia coli O45
            and O55 serogroups
  JOURNAL   Appl. Environ. Microbiol. 71 (8), 4919-4924 (2005)
   PUBMED   16085897
REFERENCE   2  (bases 1 to 14483)
  AUTHORS   Debroy,C., Fratamico,P.M., Kundrat,J., Roberts,E., Davis,M.A. and
            Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2004) Veterinary Science, Gastroenteric Disease
            Center, Wiley Lane, University Park, PA 16802, USA
FEATURES             Location/Qualifiers
     source          1..14483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O45"
     gene            59..1198
                     /gene="rmlB"
     CDS             59..1198
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-D-glucose-4,6-dehydratase"
                     /protein_id="AAX07738.1"
                     /db_xref="GI:59803191"
                     /translation="MLRFQNASIQTNTLDGTDNVKILVTGGAGFIGSAVVRHIIKNTQ
                     DDVVNVDKLTYAGNLESLSEVSDSDRYAFEHADICDKDAMDRILAKHKPDAVMHLAAE
                     SHVDRSITGPAAFIETNIVGTYVLLEAARAYWSTLDEQAKKAFRFHHISTDEVYGDLP
                     HPDEHPASTELSLFTETTAYAPSSPYSASKASSDHLVRAWLRTYGFPTIVTNCSNNYG
                     PYHFPEKLIPLVILNALDGKVLPIYGKGDQIRDWLYVEDHARALYTVVTQGKPGETYN
                     IGGHNEKQNLDVVHTICDLLDEIVPKEGSYRDQITYVTDRPGHDRRYAIDANKISAEL
                     GWTPQETFESGIRKTVEWYLTNTEWVENVKSGNYKSWIAQNYQNR"
     gene            1246..2196
                     /gene="wbhP"
     CDS             1246..2196
                     /gene="wbhP"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase"
                     /protein_id="AAX07739.1"
                     /db_xref="GI:59803192"
                     /translation="MTMKVLVTGGTGFLGSALVKALRSAGHQVIFTSRRDENIDNGMY
                     NLGEISAQTNWTTLLQGCDTVIHTAGRAHILNDKAEDTLTEFRRVNHDATMKLAHDAI
                     SCNVKHFIFVSSIGVNGNSTSGIPFSETTPARPTSDYAISKLEAEESLLKAFTGSNMG
                     ITIVRPALICGPNAPGNIQRLLKLVSRNLPLPFKSVKNKRALASLDNVVSFICECVTN
                     VKSKNELYLLADEGHPSTEEIITAFSTGMGMKARLVYFPKTILKIILSILGKKSIYDQ
                     LFGDLEVDSRKSREHLQWTPPVSLQETMVKTAKYFIEGNK"
     gene            2196..3230
                     /gene="wbhQ"
     CDS             2196..3230
                     /gene="wbhQ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAX07740.1"
                     /db_xref="GI:59803193"
                     /translation="MLYLGIVVFAFFISCALTWGLRLYAIKNNVIDQPNQRSSHSVPT
                     PRGGGVAIVLTLLASLIWLVLTNHITQATFLGFFVTGLLIAVIGFLDDHGHIAARWRL
                     LMHFIAAAIGLFYLGSFPSINMFGYDVSLSWFGMILGSIYLVWMLNLYNFMDGINGLA
                     SAQAITFSLCSILIITINNYSDSSDAMTMLALALAGSVAGFIVWNFPVARIFMGDAGS
                     GFLGITIGLMILYFAKLDSRVLIAELCLLGVFIVDATTTLLRRLLAGKKVYEAHASHC
                     YQILARKYKSHVPVTMAAIAINFMWLLPIAYLIISAKIDGIVGITIAWLPLMILAFRC
                     GAGVKDKERA"
     gene            3231..3791
                     /gene="wbhR"
     CDS             3231..3791
                     /gene="wbhR"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="AAX07741.1"
                     /db_xref="GI:59803194"
                     /translation="MDDLIIVGAGGHGRTLYETATLLGYEKIVFLDDNESRSDNSKVR
                     VIGKTSDLKLYASTAKHIVIGIGNNKLRKSFHQQLELLSIYPITLIHPFAFVSPSATL
                     GHGSVVLAGAVVGANSALGVGTIVNCHSTVDHDSTLGDFAHLGVGVHLAGGARIGKSA
                     FLQAGTVGGYSATVEEHVICPPGTIL"
     gene            3830..5716
                     /gene="wbhS"
     CDS             3830..5716
                     /gene="wbhS"
                     /note="role in galactose modification"
                     /codon_start=1
                     /transl_table=11
                     /product="TrsG protein-like"
                     /protein_id="AAX07742.1"
                     /db_xref="GI:59803195"
                     /translation="MLTYLISLPRPIKRIITLSIDVVLLMCAFWFAFWVRIDSMTPLS
                     SVSHWLLLTSTIVITLAIFVKLGLYRAVIRYITAKILLVVAIGMLISSLLLIIAAFYT
                     NLYLPRTIPFIYFSFGVLFVAGSRLAMRMLVNHGLKLGTRVIIYGAGASGRQLLPALS
                     QMDEYYPVAFVDDNRSLQGQVIHGVSVFSPEKLAWLIEKYEAKKILLAMPSASRARKN
                     EIISTIEGLQCEVLSIPGMLDLIEGKARIDALRKVVITDLLGRDPVTPIPELILKNIH
                     GKTVMVTGAGGSIGSELCRQIIRNKPFQLILFELSEFSLYSIDSELRNLILSQSLNVK
                     IYPILGNVQDMAHLERLIKAFSVETIYHAAAYKHVPLVEFNVIDGVRNNIFGTLCCAQ
                     AAENCGVESFVLISTDKAVRPTNTMGATKRMAELILQALASESSKTCFSMVRFGNVLG
                     SSGSVVPLFEKQIAAGGPVTLTHPDITRYFMTIPEAAQLVIQAGAMGSGGDVFVLDMG
                     DPVKIIDLAHRMIALQGLSVRDDKNPYGDIAIETTGLRPGEKLYEELLIGDNVSITQH
                     PRIKTANEVMLPWNEMKDIIAELDIACRNFDYEGVRKILLNAPTAFNPTDDICDLLWK
                     EHKS"
     gene            5797..6336
                     /gene="rmlC"
     CDS             5797..6336
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose-3,5-epimerase0"
                     /protein_id="AAX07743.1"
                     /db_xref="GI:59803196"
                     /translation="MNVIQTPLKDCVIIEPKVFGDSRGFFLEAWHKEKYENAGIKGNF
                     VQDNRSRSSRNVLRGLHFQKTKPQGKLVSVISGEVYDVAVDLRHDSETFGQYVSVLLS
                     GKKNNQLYVPPGFAHGFCVLSEYADFHYKCTDFYDPKDEGGIIWNDPDIAIDWPVTEP
                     LLSEKDIKLITLAEYKKSL"
     gene            6333..7151
                     /gene="wbhT"
     CDS             6333..7151
                     /gene="tll"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase"
                     /protein_id="AAX07744.1"
                     /db_xref="GI:59803197"
                     /translation="MKAYYTVIGGRGFIGSEIVENLLAKGEDVEVPPRDDKTLFERDL
                     GIVIYAAGNGDCANTPFEVYKSNTELLATLLERAQFSKLIYISSTRVYMGQDSASEMD
                     NLTVCTSDNRRLFNLTKLVSEELCLKSNRDTIIVRPSNVYGLALKSKVFLPSIIRNAI
                     SNKHIDMYVAPAYAKDYVSVTDVAELIYQISCKNEYSIYNIASGVNTTAADIADLIAS
                     KTGCTIKWHEGFKGEYFPPTDNTRTMNEFGHVYNNVLSDIEIMIDNFRKNMTDV"
     gene            7144..8406
                     /gene="wzx"
     CDS             7144..8403
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAX07745.1"
                     /db_xref="GI:59803198"
                     /translation="MSNFLLSAIKKNVADEDKRKVFVNFFYLSLERITQLVIIIIINR
                     LLINHYDFGDFSSWQYSLVILAIFMTGTWICGAEMVIPKLLDAPSNINTTMSNVIILR
                     LAAGTFVALCMVAWGFFAASGLSRQFIVGLAISVALRETFIVGLTWYQSQARLKLPCL
                     VLMLAAIIKLIVIYIGLRNQLPINYLWVAWVIESLLPCGFIFYFFRKATGFRFVKVDS
                     EIFSYLKIGVAIWCCLIMQQVTMKFDRVYLEGKISGEMYSNYAAALQLVDNWYAICIL
                     FVQAIAPIFIFKFIDIINIKRKLPFCVFATLAVTCTGALFTTVLADMIIHILYGEKLV
                     NAYSYLRTFVWLTPILAIDQLLSMVIIRMNQLNKLAIKWLIAFCLVVAVVPAIYHYMG
                     ISNIVIGLAVVYSFNIIYSTRCIGAVQ"
     gene            8387..9352
                     /gene="wbhU"
     CDS             8387..9352
                     /gene="wbhU"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAX07746.1"
                     /db_xref="GI:59803199"
                     /translation="MVQYNDKISIVVPAYCEPSKVKRFMDAILNIDYPDNLIELILID
                     DCSPVSLEEIAASYGEVFKDKINFLFHRNQINSGRAISRNVGISLATNSLIMFIDIDN
                     LLEPYAIKKIVSFFHGKHFTAARINIRIDPARLSTSNYLRYFDSRYLGARNIPEGIIS
                     TRFFASDGIILTRDIINTIGGFDETFYHYGCEDEELGIRVSKAKYDFYFLPNAKAEDS
                     DTPTLRRASERMVVYASKSFPVLKEKHPECVKDSLFSSYEVMLDDTRLRSKLLINVIH
                     LLPLTTTRKSLLWLCDKLDAKAIKVPDFIYKFVLALSYIEGGKLR"
     gene            9366..10514
                     /gene="wzy"
     CDS             9366..10514
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAX07747.1"
                     /db_xref="GI:59803200"
                     /translation="MSIFLMSPGFVYASIWLFTIILYSLGVTTNISPLSNEMVVFLSI
                     NVTLGLIFSLPFITPGSINRVRLDEIMPSWRATKIWLIIWCFVIIPDVVAAGGIPMIM
                     QFRGGYNYTEFGIPTYHGIVNMLFLFVFPSLYYHFLLSKRKIILLIILLMSIWEMLVF
                     RRGILMSGLVEIFFLFFIYKKFTKKMFIYTVFSVITIVLLFGAVGDIRGAENPYQYLL
                     SPEGQFLSSLPSGFTWFYVYVTAGLANLAYNFAHIVPVYDFTSFQDLFPSVIRNLIYS
                     DLGFHDTMELVDDNANVSTMFEKLMPDLGIAGSLIVVTCLLLLFSIAYKNLLKSYHYS
                     LFPYAIAMQCAIFSGFYNLFFIQTYFLLFIVTLVFVRIKIFTGSHSHV"
     gene            10507..11043
                     /gene="wbhV"
     CDS             10507..11043
                     /gene="wbhV"
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="AAX07748.1"
                     /db_xref="GI:59803201"
                     /translation="MFEFVKNDVLRYYKLTGRNKSKVSIFGIISAFFGMKMTPVILYR
                     MSHFCFKKKIPILGKVLSLINFVVFGVEIASNCEIGGGFFIPHSQGIVIGASKIGVNA
                     TIYQQVTLGAKNIDIPFNHLNRPVLGDNVVIASGAKVLGGIRIGDNVIVAANAVVMHS
                     CDNNVLLVGIPAVVKKSI"
     gene            11045..12055
                     /gene="wbhW"
     CDS             11045..12055
                     /gene="wbhW"
                     /codon_start=1
                     /transl_table=11
                     /product="unknown"
                     /protein_id="AAX07749.1"
                     /db_xref="GI:59803202"
                     /translation="MKVLFLCPRFFNYENEISDGLRRTGATVDYFDEKPFNNVFFKIL
                     LRLWKGNNFIKRISDAYFEKILLQTNDDYDYVIVLKGESLDRKNLLKFKNKYKNAKFI
                     YYAWDSIKNYPHIQECLNLFDRVFTFDDNDAREYDFMTHLPLFYSPDFVSTAKKEASK
                     NFKPSIAFLGTVHSDRYRVLGEVYEKYKNEYDLRFVLYFPSIVVLVGFLLTNFKSIIR
                     FKLFSFTLRSRSKKQIASFFSSADAVLDIQHPRQTGLTMRTIECLPLKRKFITTNSRV
                     KNYDFYSAENFYFIDRDNILIDSDFFEIPYNDAHLDAISRYSIDSWVKTLCSTHIRED
                     VV"
     gene            12102..12974
                     /gene="rmlA"
     CDS             12102..12974
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phospate thymidyltransferase"
                     /protein_id="AAX07750.1"
                     /db_xref="GI:59803203"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMVYYPMSVLMLA
                     GIRDILLITTPEDLPSFKRLLGDGSRFGINLSYEIQQSPDGLVQAFIIGEEFIADDKC
                     ALVLGDNIFFGQSFGRKLESVVSGNAGATVFGYQVMDPERFGVVEFDKNFKALSIEEK
                     PAAPKSNWAVTGLYFYDENVVDMAKCVKPSARGELEITTLNEMYLEQGNLQVELLGRG
                     FAWLDTGTHDSLIEASQFIHTIEKRQGFKIACLEEIAYRNGWLTESEVVTYGNEMSKT
                     NYGQYLLNLIKNSK"
BASE COUNT     4170 a   2593 c   3055 g   4665 t
ORIGIN
        1 ttattggtag ctgtaagcca agggcggtag cgtgctcaat ttctgaaatc gttaataaat
       61 gctcaggttt cagaacgcca gtatccagac aaatacacta gacggaacag ataacgtgaa
      121 aattcttgtg acgggggggg ccggctttat cggctcagcg gttgtacgac atattattaa
      181 aaatacccag gacgacgtcg tcaatgtaga taagctgacc tatgccggca accttgagtc
      241 gctgagtgaa gtcagcgaca gcgatcgata tgcttttgag catgcggata tttgcgataa
      301 agatgctatg gatcgcattt tagcaaagca taagcctgat gcggtgatgc atttggcggc
      361 tgaaagtcat gtcgatcgtt ctattaccgg cccggcggct tttatcgaaa ccaatatcgt
      421 tggcacttat gtgcttctgg aagccgcccg ggcttactgg tcaacgttgg atgagcaagc
      481 gaagaaagcg ttccgttttc atcacatttc taccgacgaa gtgtatggtg atttgccgca
      541 tcctgatgaa catcccgcat caactgagct gtcactcttc actgaaacca ccgcatatgc
      601 acctagcagc ccatattcag cgtcgaaagc ctcgagcgac catctcgttc gtgcctggtt
      661 gcgcacctat ggtttcccga ccattgtcac caactgttca aataactatg gcccgtatca
      721 tttccctgaa aaactgatcc ctctggttat tctgaatgcg ctggacggta aagttttgcc
      781 tatttacggc aagggggacc agattcgtga ctggctgtac gttgaagacc acgcccgcgc
      841 actctacacc gttgtcactc aggggaaacc gggtgaaacg tacaatattg gcggtcataa
      901 cgaaaagcag aacttggatg tggtccatac gatttgcgat ctgcttgatg agattgtgcc
      961 aaaagaggga tcttatcgcg atcagataac gtatgttact gatcgtccgg gccatgaccg
     1021 tcgctacgcc attgatgcaa ataaaattag tgctgagttg ggatggacgc cacaggagac
     1081 ctttgaaagt ggtattcgta aaaccgttga atggtatttg acaaatacag aatgggtgga
     1141 aaacgttaaa agcggtaatt ataaatcctg gattgcgcaa aattatcaaa atcgttaaga
     1201 gcagttcatt tctatatttg tttatagttc atcaactgat aggggatgac aatgaaagtt
     1261 ttagtgacag ggggaactgg ttttctcggt tctgctttag tcaaagcgct gaggtctgca
     1321 ggtcatcagg taattttcac ttcccgtcga gacgaaaata tcgacaatgg gatgtacaac
     1381 cttggcgaaa tttctgcaca aacaaactgg acaacccttc tccagggttg tgatactgtc
     1441 attcacacag caggcagagc gcatatcctt aatgataaag ccgaagatac gctgacagaa
     1501 tttcgtcgtg tgaatcacga tgccacgatg aaattagctc atgatgcaat aagttgtaat
     1561 gtgaaacatt tcatcttcgt cagttcaata ggtgttaatg gtaattccac ttcggggata
     1621 ccttttagtg aaacaacacc tgccagaccc acttcagatt atgctatttc aaagctagaa
     1681 gccgaggaaa gtcttcttaa agcgtttact ggtagcaata tggggataac catagtgcgt
     1741 ccggcattga tttgtggtcc aaatgcaccg ggtaacattc aacgattgtt aaaactagtg
     1801 tcgcgaaatc tgccactgcc ttttaaatcg gtaaaaaata agcgtgctct tgcttcactg
     1861 gacaatgttg tgagctttat ctgtgaatgt gtcacgaatg tgaagtcaaa gaatgaactc
     1921 tatcttttgg ctgacgaagg gcatccttca actgaagaaa ttataacggc attttctacc
     1981 gggatgggta tgaaagcgcg acttgtctat ttccccaaaa caatactgaa aattatcctt
     2041 tctatactgg gcaagaaaag catatatgat caactgtttg gcgatctgga agttgattca
     2101 agaaaatcaa gagagcacct tcaatggacg ccccccgtct cgttacaaga aacgatggta
     2161 aaaacagcga agtattttat tgaaggtaac aaataatgtt atatctgggc attgtcgttt
     2221 ttgcattctt tatatcatgc gcgttgactt ggggtttaag gttatatgcc atcaagaata
     2281 atgtaattga tcaaccaaac caaagaagtt ctcacagtgt tcctacgcct cgtgggggag
     2341 gggtggctat tgtattaaca ttgttggctt cattaatatg gctggtcctt acaaatcata
     2401 taactcaggc aacattttta ggtttcttcg tgactggctt attgattgcg gttattggtt
     2461 tcctcgacga tcatggccat atcgctgcac gctggcgctt gttaatgcat ttcatcgctg
     2521 cagctatagg gcttttttat ctaggatctt tcccaagcat taatatgttt ggctacgatg
     2581 tttctttgtc atggtttgga atgatcctcg gtagtatcta tcttgtatgg atgttgaatt
     2641 tatataactt tatggatggt attaatggtc ttgcaagtgc gcaagctatt accttttcgc
     2701 tttgtagtat cttgataata actatcaata actactctga ttcatctgat gcaatgacta
     2761 tgcttgcatt agcactcgct ggatcggtcg cgggattcat cgtgtggaac ttccctgtgg
     2821 caaggatctt catgggcgat gctggaagcg gttttctggg tatcaccatt gggctgatga
     2881 tcctttattt tgcaaagcta gactcacgtg ttctgatcgc agaactctgt ctgctgggtg
     2941 ttttcattgt tgatgcaact actaccttac tgagaagatt actggcgggt aagaaagtct
     3001 atgaagcaca tgcaagtcat tgttaccaaa ttcttgcccg taaatataag agccatgttc
     3061 ctgttactat ggcggctata gctattaact tcatgtggct gctccctatt gcttacttaa
     3121 tcatctctgc aaaaattgat ggaattgtag gcattaccat cgcttggtta ccattgatga
     3181 ttcttgcatt cagatgtggt gctggagtta aagataaaga gagggcataa atggatgatc
     3241 tgatcattgt tggagctgga ggacatggac gtacattgta cgaaactgct acgctattgg
     3301 gatatgaaaa aattgtattc ctcgacgata atgaatctcg ctccgacaat tcaaaggttc
     3361 gtgttatcgg gaaaacctca gatctgaagc tttatgctag tactgctaaa cacattgtaa
     3421 ttggcatagg caataataag ctcagaaagt catttcacca acagctagaa ttgctaagca
     3481 tttatccgat aacgctaatt catccttttg catttgtgag cccgtccgct actttgggac
     3541 atggcagtgt agttttagcc ggtgcggttg taggagcaaa ctcagctctt ggtgtaggta
     3601 cgatagttaa ttgtcattct acagtcgatc atgattcaac tctcggtgat ttcgcccatt
     3661 taggtgttgg ggtgcatctt gcagggggag cgcgtattgg taaatcggca tttctgcaag
     3721 cgggaacagt ggggggatat agtgcaacag tggaagagca tgtcatatgt cctccaggta
     3781 ctattttata aattaatagc aagctttaac gaggcgacta gtttttagta tgctaactta
     3841 tttaatttct ttgccaagac caataaaacg gattataacg ctctctattg atgtagtact
     3901 gttaatgtgc gcattttggt ttgcattttg ggtccgtatt gattcaatga cgccattatc
     3961 aagcgtatct cattggttac tgctaacatc gacaatagtg attactttgg ccatcttcgt
     4021 aaaacttggc ctttatagag cagtcataag gtatataact gcgaaaatac tgcttgtagt
     4081 tgctattggg atgctaattt cgtcgttgtt gctgattatt gctgcatttt acacgaatct
     4141 ctatcttcca agaacaatcc cgtttatcta cttctctttt ggagtattgt tcgttgcagg
     4201 ctcacgcctt gcaatgagaa tgttggtcaa tcacggttta aaattgggaa cccgcgtaat
     4261 tatatacggg gctggagcgt ctggacgcca gttacttcct gcgctaagcc aaatggatga
     4321 atactatcct gtggcttttg ttgatgataa tcgctcattg caggggcagg tcatccatgg
     4381 tgtaagcgta ttttcacccg aaaaattagc ttggttgata gagaagtacg aagctaagaa
     4441 aattctcctg gcaatgccaa gtgccagccg tgccagaaaa aatgagatta tatctaccat
     4501 tgagggactt cagtgcgagg tgctttcaat acctgggatg ttagacctca ttgaaggaaa
     4561 agcaagaatc gatgccttac gtaaggtagt aattactgac cttttaggtc gcgatcctgt
     4621 taccccgatc cctgaattaa ttttaaagaa tattcatggt aaaacagtca tggttactgg
     4681 cgcgggtggt tctataggct ctgaactatg tcgacagata attagaaata aaccttttca
     4741 actaattctt ttcgaattat cagaattctc actatattca attgattctg aattgcgaaa
     4801 tttaatactt tcacaatctt taaatgttaa gatttatcca attcttggca acgttcagga
     4861 tatggcgcat cttgaaaggt taatcaaggc atttagcgtt gagaccatct accatgcggc
     4921 tgcttataag catgttcctc tagttgaatt taacgttatt gatggtgttc gtaataatat
     4981 cttcgggacc ttatgttgtg cccaagctgc cgaaaactgt ggtgtcgaaa gttttgtgct
     5041 tatctctacg gataaagctg tgcgaccaac gaacactatg ggcgcaacaa aaagaatggc
     5101 agaactaata cttcaggctc tcgcatcaga gtcaagtaaa acctgtttca gcatggtccg
     5161 gtttggaaat gtgctaggtt catccggctc cgtagttcca ttatttgaaa aacaaattgc
     5221 tgctggtggt ccggtgacgt taacgcatcc tgacatcaca cgttatttca tgaccattcc
     5281 tgaggcagca cagcttgtta ttcaagctgg agcaatgggc agtggtgggg atgttttcgt
     5341 attggatatg ggggatccgg tcaaaattat tgacctcgcc caccgtatga ttgccttaca
     5401 aggactttca gttcgcgatg ataaaaatcc ttatggcgat attgctatcg aaacgactgg
     5461 cttgcgtccg ggtgagaagc tgtatgaaga gttgttgatt ggcgataacg tctcaataac
     5521 acagcatcca cggattaaga cagctaatga agtcatgctt ccgtggaatg aaatgaaaga
     5581 cattatcgca gaattagata tcgcatgtcg caactttgat tatgaggggg ttcgcaagat
     5641 attgctaaat gcacctaccg cgtttaatcc tactgatgat atctgtgact tactttggaa
     5701 agaacacaaa agttaagtat ttatagtttc aatccgtgtt cgaaattaac taacaaagca
     5761 gcttgtgagt atatctttcc agctgaggaa ttttaaatga atgtaattca aacaccttta
     5821 aaagattgtg tgatcattga acctaaggtt tttggcgatt ctcgcggttt tttccttgag
     5881 gcctggcata aagaaaaata tgaaaatgca ggaatcaaag gtaattttgt acaggataac
     5941 cgctcacgtt cgagtcgaaa tgttttgcgt ggattgcatt tccagaaaac gaaaccccag
     6001 ggtaaattag tcagcgtaat cagcggggaa gtttatgatg ttgcagtgga tttgcgccac
     6061 gattctgaga cgtttggtca atatgtgagt gttcttcttt ctggcaagaa gaataaccaa
     6121 ttgtacgttc ctcccggatt tgcccatgga ttttgtgtat taagcgaata tgccgatttt
     6181 cactataagt gtactgattt ttacgatcca aaggatgaag gtgggattat ctggaatgat
     6241 ccggatattg cgattgactg gccggtaaca gaaccacttc tctcagaaaa agatattaaa
     6301 ttaattacac ttgcagagta taaaaaatct ttatgaaagc atattataca gtgatcggtg
     6361 gacgtggttt cattggcagt gaaatcgttg aaaatctttt ggctaagggt gaggatgttg
     6421 aggttccacc gcgtgatgat aagactcttt ttgaaagaga tctgggaatt gtcatctatg
     6481 ctgctggcaa tggtgattgt gcaaacacac cgtttgaagt atataaatct aacaccgagt
     6541 tgctggcaac gcttttggaa cgcgctcaat tctcgaaatt aatctatatt tcatcaacgc
     6601 gtgtttacat gggacaagat agcgcgtcgg aaatggataa tcttacggta tgcacgagcg
     6661 ataaccgaag gcttttcaat cttactaaac tggtaagtga agagttatgc ttaaaaagca
     6721 accgagatac gattattgtc agacctagta atgtatatgg tttggcatta aagagtaagg
     6781 tattcctacc tagcataatc agaaatgcaa tctcgaataa acatattgat atgtatgttg
     6841 cccctgccta tgcaaaagat tatgtttcag tcacggacgt tgctgaactt atttaccaga
     6901 tcagctgtaa aaatgagtat agcatctaca atattgcttc aggtgttaat acaacagctg
     6961 ctgatattgc agacttaatt gcaagtaaaa ctggttgcac gatcaaatgg catgaaggtt
     7021 tcaagggtga atatttcccg ccaacagata ataccagaac catgaatgag tttggccatg
     7081 tctataacaa tgtattatca gatatagaaa taatgattga taactttaga aagaatatga
     7141 cagatgtcta attttttatt atcagccata aaaaaaaatg tggcggatga agataaaaga
     7201 aaagtctttg taaacttctt ttatcttagc ctcgagagaa tcactcaact tgttatcatt
     7261 attatcatta acagattatt gataaatcac tatgattttg gtgatttcag cagttggcaa
     7321 tattctctcg tcattttagc catatttatg acaggcacat ggatctgtgg agccgagatg
     7381 gtaattccta aattactgga tgcaccatct aacataaata ccacaatgag caacgtgatt
     7441 atattacgtc tggctgcagg gactttcgtt gcgttgtgca tggtggcatg gggatttttt
     7501 gctgcaagtg ggctgtccag acagttcata gttggtttgg ccatatctgt cgcattacga
     7561 gaaactttca tcgttggttt aacatggtat caaagccagg ctcgtctcaa attaccttgt
     7621 ctggtactta tgttagctgc aataattaaa ctcattgtta tttatattgg tttaaggaat
     7681 cagttgccaa taaattatct ttgggtagca tgggtcattg aatcactttt accttgtggc
     7741 tttatctttt attttttcag aaaggctacc gggtttcgat ttgtgaaggt tgattctgaa
     7801 attttctcat atcttaagat tggtgttgcc atatggtgtt gtttgatcat gcaacaggtc
     7861 actatgaagt ttgatcgtgt ttatctggaa ggtaaaattt ccggagaaat gtattcaaac
     7921 tatgcagcag cacttcaatt ggtcgataac tggtatgcaa tatgtattct gtttgtccag
     7981 gcaattgcac caatttttat atttaaattc attgatataa taaatattaa aagaaaattg
     8041 cctttttgtg ttttcgctac acttgcagta acctgcacgg gcgcattatt tacaacagtt
     8101 cttgcagaca tgatcattca tattctttat ggtgaaaagc ttgttaatgc ctatagttat
     8161 ttgcgaacgt ttgtctggtt aaccccaatt ttagctatag atcaactgct cagcatggtt
     8221 ataattagaa tgaatcagct aaataaatta gctattaagt ggttgatagc tttctgccta
     8281 gtagtggctg ttgtgcctgc tatttaccat tatatgggta ttagtaacat cgtcatcggt
     8341 ttagcagtag tttactcctt caatataatt tattctacga ggtgcattgg tgcagtacaa
     8401 tgataagatt tcaatagtgg tccctgccta ttgtgaacca tcgaaagtta agcgtttcat
     8461 ggatgccata ctgaatattg attatccaga taaccttatt gaattaattc ttattgacga
     8521 ttgtagtccg gtttcactgg aagaaatcgc agcctcttat ggagaagtct ttaaagataa
     8581 aataaatttt ttattccaca ggaatcagat aaattctggc agggctattt cccgcaatgt
     8641 tggtatttct ttagcgacaa actcgttgat aatgtttatt gatatcgata acctgcttga
     8701 accttatgca ataaaaaaaa ttgtttcttt tttccatggt aagcacttca ctgcagccag
     8761 aattaatata cgaatcgacc ctgccagact ttccactagc aattatctcc gttattttga
     8821 tagccgttat ctcggagcgc gtaatatccc tgaagggatc ataagtactc gtttctttgc
     8881 aagtgacggt attatcctga cacgagacat cattaataca atcggtggtt ttgatgagac
     8941 gttctatcat tacggctgtg aagatgagga gcttggaatc cgagtttcaa aagcgaaata
     9001 tgacttttat tttttaccca atgcaaaggc agaagacagt gatacaccaa cattgcgccg
     9061 agcatcagaa agaatggttg tgtatgcatc aaaatctttc cctgtattga aagaaaaaca
     9121 cccggagtgc gtgaaggata gtctcttttc atcctacgag gtaatgttag atgatacgag
     9181 actccgcagt aaattactca taaatgttat tcatcttctt cctcttacaa caacccgcaa
     9241 aagccttctt tggttgtgtg ataaattaga tgcaaaagcg attaaagttc ctgacttcat
     9301 atataagttc gtattggcac tttcatatat tgaaggtgga aaacttcggt agctgaggat
     9361 ataggatgag tattttttta atgtccccag ggtttgtgta tgcttcaatt tggctgttca
     9421 caattatatt atattcgctt ggagtgacta ctaatattag cccgctctca aatgagatgg
     9481 tggtgttttt atcaattaat gttactctgg gtttgatttt ttcacttcca tttattactc
     9541 ctggcagtat taatcgcgtt cgtctggatg aaattatgcc atcttggcga gcgactaaaa
     9601 tatggctcat catttggtgc tttgtgataa ttcctgatgt ggttgctgct ggcgggatac
     9661 caatgattat gcaatttagg ggcggctata attataccga gttcggtata ccgacatatc
     9721 atgggatagt taatatgcta ttcctttttg tttttccgtc actgtattat cattttcttt
     9781 tatcaaaaag aaaaattatt ctgctgatta tactattgat gagtatatgg gaaatgctgg
     9841 tcttcagacg aggtatactg atgtcaggcc tcgtggaaat attcttttta ttttttatct
     9901 ataaaaaatt cactaaaaag atgtttattt atacagtatt ttctgtaatc acaatagttc
     9961 tactttttgg agctgtagga gatatcaggg gcgctgaaaa tccttatcaa tatttgttgt
    10021 caccagaagg acaatttctg tcgagtttgc cttcaggctt cacatggttt tatgtctatg
    10081 ttacggcagg ccttgcaaac cttgcatata actttgcgca catagttccg gtatacgatt
    10141 tcacaagctt ccaggaccta ttccccagtg ttatcagaaa tctcatttac agtgatttag
    10201 gattccatga taccatggag ctagtagacg ataatgccaa cgtttcgaca atgtttgaaa
    10261 agctaatgcc cgatcttggg atcgcgggct cccttattgt ggttacatgt ttacttctgc
    10321 tttttagtat tgcttacaag aatttgctta aatcgtatca ttatagttta tttccttatg
    10381 cgattgcaat gcaatgtgca atattcagtg gcttttataa tttattcttt attcaaacat
    10441 attttttact tttcattgtt actcttgttt ttgtgagaat taaaatattt acagggagcc
    10501 attctcatgt ttgagtttgt taaaaatgat gtgctgagat attacaaact gactgggcga
    10561 aataaatcaa aagtatctat ctttggtata atatcggctt ttttcggaat gaaaatgact
    10621 ccagttattt tataccgtat gagccatttt tgctttaaga aaaaaatacc tattttaggg
    10681 aaagttcttt ctctaataaa ttttgtcgta tttggtgttg agattgcttc taattgcgaa
    10741 ataggtggtg gtttctttat tcctcattcg cagggcattg ttattggtgc atctaaaatt
    10801 ggtgttaacg ctacgatata ccaacaagtt actctgggcg caaaaaatat agatattccc
    10861 tttaatcacc taaatcgccc tgttttaggt gataatgtgg ttattgcatc tggggctaag
    10921 gttcttggcg gtattagaat tggcgacaat gttattgtcg ctgctaatgc tgttgtaatg
    10981 cattcctgtg ataacaatgt gctacttgta ggcatcccgg ccgttgtgaa gaagagtatt
    11041 tgaaatgaaa gtgttgtttt tatgtcctcg tttttttaac tatgaaaatg aaatatctga
    11101 tggattaagg cgaaccggtg ccaccgttga ttatttcgat gagaaaccat ttaataacgt
    11161 attttttaaa atattactca gactttggaa aggtaataat tttattaaac gtatttcaga
    11221 cgcgtatttt gagaagattt tgcttcaaac aaatgatgac tatgattacg tcatagtact
    11281 caaaggtgaa tctcttgata gaaaaaattt attgaaattc aaaaataaat ataagaacgc
    11341 taaattcatt tattacgcat gggattctat taaaaactac cctcatattc aagagtgcct
    11401 gaatttattt gaccgcgtat ttacctttga tgataacgat gcgcgagaat atgattttat
    11461 gactcatttg ccattgtttt attccccgga ttttgtaagc acagcgaaaa aagaagcttc
    11521 aaagaatttt aagccatcta ttgcatttct cggtaccgta catagtgaca gatacagagt
    11581 attgggagag gtttatgaga aatataaaaa tgaatatgat ttgaggtttg ttctttattt
    11641 cccttcaatt gttgtcttgg taggttttct tcttactaat tttaagtcaa tcattaggtt
    11701 caaacttttt agttttaccc ttcgctctag aagtaaaaag cagatagcat cattcttctc
    11761 tagtgctgat gcggtccttg atatccagca tccccgtcag acaggtttga cgatgaggac
    11821 aatagaatgc ttgcctctca agaggaaatt tataactacc aattcacgag ttaaaaatta
    11881 cgatttttat tccgctgaaa atttttactt tatcgatcga gataatatcc ttatcgactc
    11941 tgattttttc gaaatcccat ataacgatgc gcacctagat gcaatttcgc gttatagtat
    12001 agactcgtgg gtaaaaacat tatgttcgac tcatatcaga gaagatgttg tttaaataaa
    12061 gatatctaat ctatttaact taaaagtatt gaggtggtaa aatgaaaggc attatcttgg
    12121 ctggcggttc tggaacacgt ttgcacccga taactcgtgg agtctctaaa caactcttac
    12181 ctatttatga taaacctatg gtttattatc caatgtcggt gcttatgctt gcgggaattc
    12241 gagatattct cttaatcaca acacctgaag atttgccttc ttttaaacgt ttactgggtg
    12301 atggcagtcg tttcggaatt aatttatcct atgaaattca gcaatctcct gatggcctcg
    12361 tgcaagcatt cattattggt gaagagttta tcgcggatga taaatgtgct cttgtattag
    12421 gcgataacat tttcttcggg caaagtttcg gcagaaaact tgaatcggtt gtttccggca
    12481 atgccggagc tactgttttt ggttatcagg taatggatcc agaacgtttt ggagttgtcg
    12541 agtttgataa aaacttcaag gcattaagta tagaagaaaa gccagctgcg cctaaatcca
    12601 actgggctgt tactggccta tacttttacg atgaaaacgt ggttgatatg gctaaatgtg
    12661 tcaagccatc agcacgcggt gaacttgaaa ttactacact gaatgaaatg tatctcgagc
    12721 aggggaatct tcaggtagaa ttgctcggcc gtggttttgc atggctcgat actggtaccc
    12781 atgatagctt aatcgaagca tcacaattta ttcatactat agagaaacga cagggattca
    12841 aaatcgcctg cctggaagaa attgcttacc gtaacggctg gctcactgaa agtgaggtcg
    12901 tgacctacgg gaatgagatg agcaagacca attacggtca atatttgctc aatttaataa
    12961 agaatagtaa atagtagtta ttgttatcta acaatatcat atctttcatg gggatctagt
    13021 aataattcta aaatgaaagc aaaatatttt ttctttacct ctgtacttct gttaccttct
    13081 gcgttctccg cgaaaaacag caatgcaatt ttctcttagg cacggtatca tcatcctact
    13141 aagttgaagg tggttggaat aaaaatggaa aggggccttc agattgggat tattttaccc
    13201 ataatgaggt cacaaaatat accattggca ctgttggaaa tttaccactg gaacacgcca
    13261 ttagcacttt ataaaaaagg aagttggtct aatcctgaat caccagtttg gtttgagaaa
    13321 tatgcacaag tgatttttga aaattttggt agcagaagtt attattttct aacttttaat
    13381 gagcctgaag gatacgtttt tactcaggaa cctcttgcag caaaccttat tgataaaaag
    13441 ctagacggat atcgtgatgt tttatccgtt gtttctcgtg gaaaataagc gatagcattt
    13501 cacaatcttt tgatcgctaa tgcgatggtt gtacgtaatt atcacaaagc aaaatatgat
    13561 ggacgtattg gcgtattggg attgcactaa acttatcgcc aagcattgat aatgaccaac
    13621 caaatagtgc tactgagaag ttatgaaacg atattcataa caactagatt ctggatgtta
    13681 tttataagag ggaataccct cattcagtat tgaaaatgta ttagtctaca tatcctaaat
    13741 tcaagcctac atttcaagat atgcaattca ttacctaggg aaaacctgat tttatcggcg
    13801 taaattgtta tggtccaaca ttggttaagt atgataaatc tgaaccgttt gaaatatgta
    13861 acgtttcaaa tccagataaa gggccttctg tcaacggtcc attctctcca gaggcgctgg
    13921 taatgatgat gaaaaagttt gatatacaat attcgcatcc taccttcatt attactgagt
    13981 atggtgcagg ttttggttta gccgataaaa aatttaatga tggcattatt tctgataagt
    14041 tgcgagtaga ctatctcaag agatatgtat ctgcagttat tgaagcaaaa aagaaggttt
    14101 agatatccat ggctatctat tttggagctt gcttgataat ttcgaatagt tatagggtta
    14161 caaaaatagg tttggcataa ttggtgttga ttttaatgat aaggctttga agcgaacacc
    14221 ggagttaagc tattatagct accagaaaat tattaaagaa aataaaaatc aatgtgtttt
    14281 attgatgaga aaataatcca caataaacca tgtcttttgt taaggcttaa gtgtgtcaat
    14341 gaccataatt acctacctct gctctcgatt tctgagttaa gataattcga taatatatcc
    14401 accgggaagt accccggtgg gagcacacct gacaggagta tgtaatgtca aagcaacaga
    14461 tcggcgtcgt cggtatggca gtg
//
