LOCUS       AB811621               13542 bp    DNA     linear   BCT 13-DEC-2014
DEFINITION  Escherichia coli DNA, O-antigen biosynthetic locus, strain: P1c.
ACCESSION   AB811621
VERSION     AB811621.1  GI:733166379
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. (2014) In press
   PUBMED   25428893
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13542)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..13542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="P1c"
                     /db_xref="taxon:562"
                     /note="serogroup:O46"
     gene            <1..899
                     /gene="wcaM"
     CDS             <1..899
                     /gene="wcaM"
                     /codon_start=3
                     /transl_table=11
                     /protein_id="BAQ00951.1"
                     /db_xref="GI:733166380"
                     /translation="AILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISD
                     HVIERINCTNDKINWGIGIGLSGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENG
                     KHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKG
                     KYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQ
                     HLFMRNINVMQESSVGPALIMNFDMRKDVRGVFMAKRETLLSLANIHAVNEMGQNSVD
                     IDRINHHIVNVEKINFRLPERR"
     gene            1060..2055
                     /gene="gne"
     CDS             1060..2055
                     /gene="gnu"
                     /note="O046_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAQ00952.1"
                     /db_xref="GI:733166381"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            2298..3191
                     /gene="galF"
     CDS             2298..3191
                     /gene="galF"
                     /note="O046_02"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ00953.1"
                     /db_xref="GI:733166382"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGELSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     CDS             3499..4344
                     /note="O046_03"
                     /gene="wfdR"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00954.1"
                     /db_xref="GI:733166383"
                     /translation="MINVRKIAKLIIGDNLYWKLKFFYATKKWPNIYDPTFFNEKVRW
                     RMLHDQNYLYVKCADKYEVRNYIEKKIGSEYLVPLRYMFTNNDDITDKCILSNSVAKS
                     NHGAGMIEFIPETYSLSELKNTFGKWLNLDYTSESNEKHYSRIPRKILIEESLCKEGK
                     PPVDYKFHTFKQADGSFRFVLQLVNGRFHNESRGYYLEDLNKCIWSHGEGFHSIPQEH
                     IKPLKKAMELSSILCEDFNYVRVDWYIYDNKLYFGELTFTPGAGSSYEFGNELEKLMA
                     KYWQL"
     gene            4346..5476
                     /gene="wzx"
     CDS             4346..5476
                     /gene="wzx"
                     /note="O046_04"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ00955.1"
                     /db_xref="GI:733166384"
                     /translation="MKIIYNTLWMIFEKLVLSLSLLIINAYVARYLGPQQYGVIAYSI
                     TLLSISVVISNFGTNTLIFQKISKNHSVAKIIIPPIQILKTILYIICILIMFFCLYIF
                     SYDFDPYLTFGLAVAFYFQAIDSYTWYNDAKLKSKYNSLINTIALIGAIISRYYLVKL
                     SMDLKWFAIPYVCNYGISFLLKRYFYIKEEKTKSIKSFIFEKKDHTLKVISFFIISGA
                     PLFLSEISILIYTRLTNLYLGQIKDIDAVGIFNASYVLATAWTVVPLAFVTSVFTIIY
                     KEKDISNRALIGTFLTLLMLFFGFLLVILCYFFKQQIISIVWLRFRVIFFYITCTYSW
                     LSFLSFRCYFLSDDNINAWVQIYSKKDGYYGGDKYSIKLLSY"
     CDS             5647..6552
                     /note="O046_05"
                     /gene="welQ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00956.1"
                     /db_xref="GI:733166385"
                     /translation="MKDQALVSIIIPTYGRCDTLNLAVQSVLKQDYKNIEVLVIDDND
                     NPIMSKKVRANLSEYQSDPRLKYFSDGVNRGGAGARNKGIELSIGYYITFLDDDDLFL
                     ENKISKQIDFLEKEQLDVCLCDMYFKQGDRFIKKSNCYANGTTLKEFILKGNCYTPMI
                     MCKRHVLIDIGGFVLTPRFQDHLLMIRILSNTSKVGHLSEQLFVHNNHNGERITYSNK
                     SAEAYILREKMEREYIYLLNSNERKTFEFKKALIRMKIYRLKNEHMKLLKITLYAMKN
                     LTNFSDVYALGKTLIRVMFFGSKNV"
     gene            6549..7688
                     /gene="wzy"
     CDS             6549..7688
                     /gene="wzy"
                     /note="O046_06"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ00957.1"
                     /db_xref="GI:733166386"
                     /translation="MKTNNKKIKALIFFISIFLYFFKSVFISLELPEDLIGGVSNIIN
                     ALILAILSPVILLSNKKQCAFIIFLIILFVYNAIFYNNIYMFGFIIIACMLILSNGIY
                     WRDIIRYLSLAHFLVFLFIIPLVFFAEKYSFIDDRFGVRYTFGFHNPNTFSQYLISLY
                     VVFLLLFVEVIKKKSLQFLTVIILTLFIYGVISLTGSRTGMILTIITGFGFLACTLSK
                     TNDKKRRKFVYLYILGAGILCFIQYYLALTYNHSEFSKNINTILSGRIWFSSQLTSQL
                     WPVPYFHGVNINDYLPIDFFYVAYFYNLGIFIGSWFIYLFIRKMFVQTYTPVMVIALW
                     LSLAITVTENYYAIPLYNISLFIVFSSRYVINENENSCTYPSSQK"
     CDS             7651..8478
                     /note="O046_07"
                     /gene="welR"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00958.1"
                     /db_xref="GI:733166387"
                     /translation="MKTAVLIQAHKNENYIRELALNNPSVRFYVHMDAKYPNKIQWIK
                     NECIDNIYLIENPVSVYWGGSSQIFATLLLMKKAYSDKRNKFFHLVSSECVPLKSFVE
                     IENEWSMNENCQFIESHRDKNNEWRLKVRVPHSNTKYLRTFLGRCANKLFKVTTFLWD
                     SVGLNPENYFFGSQWFSVTRNFVEQVIDEDNEDFFKTFHNITCADEHAFAIFARTKYS
                     NPNDIQDNNKRFIKFLGKSSPEYLSLDQVGQIKNLNSYWFCRKVRCNDLLKIIRQEK"
     CDS             8481..9209
                     /note="O046_08"
                     /gene="wbdN"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00959.1"
                     /db_xref="GI:733166388"
                     /translation="MLFSIIMPAYNSQATIKESIASVLNQTYQNFELIIINDNSSDAT
                     LSIITNFCHDRRILVLNNEENMGVAHSRNRGLEMASGEIIAFLDSDDIWYPNKLEEQY
                     NCFLSGHKIVCSYYDVIDSEGDIVGTRNAPTLVTFEKMLKSNFIGNLTGAYASSFFGK
                     CYQRNIGHEDYIMWLELVKKQPAYCIKNKLAAYRISNKSLSSNKMKVVIWQWKIYRKV
                     LGMNIIKSLYYFLNYIYFAIKKRN"
     gene            9328..10734
                     /gene="gnd"
     CDS             9328..10734
                     /gene="gnd"
                     /note="O046_09"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ00960.1"
                     /db_xref="GI:733166389"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAELIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            10983..12149
                     /gene="ugd"
     CDS             10983..12149
                     /gene="ugd"
                     /note="O046_10"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ00961.1"
                     /db_xref="GI:733166390"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            12239..13273
                     /gene="wzz"
     CDS             12239..13273
                     /gene="wzz"
                     /note="O046_11"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ00962.1"
                     /db_xref="GI:733166391"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQASLIGRYSTAFSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSSNYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(13369..>13542)
                     /gene="hisI"
     CDS             complement(13369..>13542)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="BAQ00963.1"
                     /db_xref="GI:733166392"
                     /translation="AQKVGEEGVETALAATVHDRFELTNEASDLMYHLLVLLQDQDLD
                     LTTVIENLRKRHQ"
BASE COUNT     4142 a   2451 c   2845 g   4104 t
ORIGIN
        1 acgccatctt acgccaggga tttcataacc agattatcgg tgccaacatc accaattgta
       61 agttcagcga cttacagggg gacgctattg aatggaacgt ggcgattaac gacagtgata
      121 ttttgatctc cgaccatgtc atcgagcgca tcaactgtac taacgacaaa atcaactggg
      181 gcatcggcat aggtctttcg ggtagtacct acgataacaa ctacccggaa gaccaggcag
      241 tgaaaaactt tgtcgtggcg aatatcacgg gatcggattg tcggcagttg atccatgttg
      301 aaaatggtaa acattttgtt attcgtaata tcaaagcccg caatatcacg ccggatttca
      361 gtaagaaagc aggcattgat aacgcgacag ttgctattta cggttgtgac aatttcgtga
      421 ttgataatat tgaaatgatt aatagcgccg ggatgttaat cggctatggg gtaattaaag
      481 gcaaatatct ctcgataccg caaaatttcc gagtgaataa tattcaactg gataacactc
      541 accttgctta taaattgcgc ggcatccaaa tctccgccgg gaatgctgtc tcctttgtgg
      601 cgctgactaa cattgagatg aagcgtgcgt cgctggaact gcacaacaag ccgcaacatc
      661 tttttatgcg taatatcaat gtgatgcagg aatcctctgt tggaccagca ttgatcatga
      721 acttcgacat gcgcaaagac gttcgcggcg tctttatggc aaagcgtgag acgctgcttt
      781 cacttgcgaa tattcatgcg gtgaatgaaa tggggcaaaa ctccgtcgat atcgacagga
      841 ttaatcacca tattgttaat gtggaaaaga ttaactttag attgccggaa cggagatagt
      901 agatttgcga ccattcataa acttactgga tataatgttc taaaagatta taatcctcac
      961 actaactgtt aaagctgggg ctgggcagca ttgactactc agggaaggta ctcatcttta
     1021 tcttgtgttt cttacgtaac aaccagacta aagtgattta tgaacgataa cgttttgctc
     1081 ataggagctt ccggattcgt aggaacccga ctacttgaaa cggcaattgc tgactttaat
     1141 atcaagaacc tggacaaaca gcagagccac ttttatccag aaatcacaca gattggcgat
     1201 gttcgtgatc aacaggcact cgaccaggcg ttagccggtt ttgacactgt tgtactactt
     1261 gcagcggaac accgcgatga cgtcagccct acttctctct attatgatgt caacgttcag
     1321 ggtacccgca atgtgttggc ggccatggaa aaaaatggcg ttaaaaatat catctttacc
     1381 agttccgttg ctgtttatgg tttgaacaaa cacaaccctg acgaaaacca tccacacgat
     1441 cctttcaacc actacggcaa aagtaagtgg caggcagagg aagtgctgcg tgaatggtat
     1501 aacaaagcac caacagaacg ttcattaacc atcatccgtc ctaccgttat cttcggtgaa
     1561 cgcaaccgcg gtaacgtcta taacttgctg aaacagatcg ctggcggcaa gtttatgatg
     1621 gtgggcgcag ggactaacta taagtccatg gcttatgttg gaaacattgt tgagtttatc
     1681 aagtacaaac tgaagaatgt tgccgcaggt tatgaggttt ataactacgt tgataagcca
     1741 gatctgaaca tgaaccagtt ggttgctgaa gttgaacaaa gcctgaacaa aaagatacct
     1801 tctatgcact tgccttaccc actaggaatg ctgggtggat attgctttga tatcctgagc
     1861 aaaattacgg gcaaaaaata cgctgtcagc tctgtgcgcg tgaaaaaatt ctgcgcaaca
     1921 acacagtttg acgcaacgaa agtgcattct tcaggttttg tggcaccgta tacgctgtcg
     1981 caaggtctgg atcgaactct gcagtatgaa ttcgtccatg ccaaaaaaga cgacataacg
     2041 tttgtttctg agtaatgcag acgagtcaga gaaaaaagga ccaggttgga ctacgattat
     2101 tcggaaagag gtactgatta aattatcagt atgcggaatt ccgagtaatc cgagtaaccc
     2161 agatgctgat ggtgtgtagt gttatcgcaa ggtttaatca aagttatgcg gaactaagct
     2221 ttggtatata gccataccgt ttggctaatc tatggcaata atttacgcac tatcagtcac
     2281 ttagaggtag gaaaataatg acgaatttaa aagcagttat accggtagcg ggtcttggga
     2341 tgcatatgtt gcctgccact aaggcgatac ccaaagagat gctaccgatc gtcgacaagc
     2401 caatgattca gtacattgtt gacgagattg tggctgcagg gatcaaagaa atcctcctgg
     2461 taactcacgc gtccaagaac gcggtcgaaa accacttcga cacctcttat gaattagaat
     2521 ctctccttga gcagcgcgtg aagcgtcaac tactggcgga agtgcaatct atctgcccgc
     2581 cgggcgtgac tattatgaac gtgcgtcagg gcgaaccttt aggtttaggt cactccattt
     2641 tgtgtgcacg acccgccatt ggtgacaatc catttgtcgt ggtgctgcca gacgttgtga
     2701 tcgacgacgc cagcgccgac ccgctacgct acaaccttgc tgccatgatt gcgcgcttca
     2761 atgaaacggg acgcagccag gtgctggcaa aacgtatgcc gggtgagctc tctgaatact
     2821 cagtcattca gacaaaagaa ccactggatc gtgaaggtaa agtcagccgc attgttgaat
     2881 tcatcgaaaa gccggatcag ccgcagacgc tggactcaga catcatggcc gttggtcgct
     2941 atgtgctttc tgccgatatt tggccggaac ttgaacgtac tcagcctggt gcatggggac
     3001 gtattcagct gactgatgcc attgccgagc tggcgaaaaa acagtccgtt gatgccatgc
     3061 tgatgaccgg cgacagctac gactgcggta aaaaaatggg ttatatgcag gcgtttgtga
     3121 agtatggact acgcaacctg aaagaagggg cgaagttccg taaagggatt gagaagctgt
     3181 taagcgaata atgaaaatct aaccgaatgt aacggttgac aagaaaatta taacggcggt
     3241 gaagattcgt ggcgaaagta atttgttgcg aatattcctg ccgtagtttt atataaacaa
     3301 tcagagtaac aacgagttag caataggatt ttaatcaaag ttttccagga ttttccttgt
     3361 ttccagagct gattggtaag acaattagcg tttgaatttt tcgggtttag cgcgagtggg
     3421 taatgctcgt cacatcgtag gcatgcatgc agtgctctgg tagctgtaaa gccaggggcg
     3481 gtagcttggt aaaaaatcat gataaatgta agaaaaatag caaaattaat tattggcgat
     3541 aatttgtatt ggaagttgaa gtttttttat gccactaaaa aatggcctaa tatttatgat
     3601 ccaacttttt ttaatgaaaa agttcgctgg cgaatgctgc acgaccaaaa ttatctttat
     3661 gttaaatgcg ctgataagta tgaggtcagg aactatattg agaaaaaaat tggcagtgaa
     3721 tatttagttc cgcttcgtta tatgtttact aataatgatg atattactga taaatgtatt
     3781 ctgagtaata gtgtagcaaa gtctaaccat ggtgcaggta tgatagagtt cattcctgag
     3841 acgtattcat taagcgagtt gaagaatact tttggaaaat ggctaaatct tgactatacc
     3901 tccgagtcca atgaaaaaca ttatagtagg attccgagaa aaattcttat tgaagagtct
     3961 ttgtgtaagg agggaaaacc tcctgtcgat tataaatttc atacatttaa acaagcagat
     4021 ggttcatttc gttttgttct tcagttggta aatggtcggt ttcataatga atcaagaggt
     4081 tattatctgg aggatcttaa taaatgcata tggtctcatg gtgaaggttt tcattctatt
     4141 ccacaagagc atataaaacc tttaaaaaag gctatggaat tgtcatcaat tctatgtgaa
     4201 gattttaatt acgtacgagt tgattggtat atctatgata ataagcttta ttttggtgaa
     4261 ttaaccttta cacctggtgc tggaagttca tatgaatttg gtaatgagct tgaaaaatta
     4321 atggccaaat attggcagtt gtaaaatgaa aataatatat aacacacttt ggatgatatt
     4381 tgaaaaatta gtattatccc tatctttatt aatcattaat gcctatgttg cgagatattt
     4441 agggcctcag caatatgggg ttatagccta ttctattaca ctgctcagta tttctgtggt
     4501 tattagtaat tttggcacta acacattaat tttccaaaaa ataagcaaaa atcacagtgt
     4561 tgcaaaaatc attataccgc cgatacagat attgaaaaca atattataca taatatgtat
     4621 acttattatg tttttctgtt tatatatttt tagttatgat tttgatcctt atctgacatt
     4681 tggtttagct gtagcatttt actttcaagc tattgatagc tatacatggt ataatgatgc
     4741 aaaattgaaa tcaaagtaca atagtcttat taatacaata gccttaatag gtgctattat
     4801 aagtcgttac tatttggtaa aactatcgat ggatttaaaa tggtttgcta ttccttatgt
     4861 atgcaattac gggatatctt ttttactgaa aagatatttt tatattaaag aagaaaaaac
     4921 taaaagcatc aaatcattta tatttgaaaa aaaagatcat acactaaaag ttatatcttt
     4981 ctttataatt tcaggtgcgc cattattttt aagtgaaata tcaattctta tttatacaag
     5041 acttacaaac ttatatctag gacaaattaa agatattgat gcggtcggca tattcaatgc
     5101 atcttatgtt ttagctactg cctggacagt cgttcctttg gcatttgtta cgagtgtctt
     5161 tactataatc tataaagaaa aggatatttc taacagggct ttaattggaa cattcttgac
     5221 tttgttgatg ttgttttttg gttttttact tgtcatattg tgttattttt tcaaacaaca
     5281 gattatcagt attgtatggc tccggtttcg agttatcttc ttctatatta cctgtactta
     5341 ttcttggctc agttttctct cttttaggtg ttatttccta tcggatgata atatcaatgc
     5401 atgggtacaa atatatagca aaaaagatgg ttattatggg ggtgataagt attccattaa
     5461 attattatct tattaaatcg ttaggtatta ttggtgcagc ttatgctaca gttagtattg
     5521 aacttatatc agccacattg gcgaattatt tttttaaagg tggggtgatt gcaaaaatgc
     5581 atttaaaaat gataacgtct ccctacagtg tatctatgca agctataact aaaatactga
     5641 aataaaatga aagatcaagc attagtctct attattattc caacatatgg gcggtgtgac
     5701 actttaaatc ttgctgtcca aagtgtacta aaacaggatt ataaaaacat agaagtattg
     5761 gttattgatg ataatgataa tccaataatg tctaagaaag ttcgtgccaa tttatcggaa
     5821 tatcaatccg atcccagatt aaagtatttt agtgatgggg ttaatagagg tggagcaggg
     5881 gcaagaaata aaggaattga attgtcaata ggttattata ttacttttct tgatgacgat
     5941 gatttgtttt tagaaaataa aatatcaaag caaattgact ttttagaaaa agaacaactt
     6001 gatgtatgcc tttgtgatat gtattttaaa caaggagacc gttttataaa aaaatcaaat
     6061 tgctatgcaa atggaacaac attaaaagaa tttatactca agggaaattg ttacacgcca
     6121 atgatcatgt gtaaacgaca tgttctaatt gatattggtg gattcgtttt aactcctcgg
     6181 tttcaagatc acttattaat gattaggatt ctttctaata cttcgaaagt ggggcattta
     6241 tctgagcaat tatttgttca taataaccat aatggtgaaa ggattacata tagtaataaa
     6301 tctgctgaag catatatatt acgcgaaaag atggagcgag aatatattta tttattaaat
     6361 tcaaatgaac gtaaaacatt cgaattcaag aaggcactta taaggatgaa aatatataga
     6421 ttaaaaaatg aacatatgaa gttgcttaaa ataacattat atgccatgaa aaacctcact
     6481 aacttttcag atgtttacgc attaggaaaa actttaataa gagtgatgtt ttttgggagt
     6541 aaaaatgtgt gaaaacaaat aacaaaaaaa taaaagcatt gatttttttt atatcaatat
     6601 ttctctactt tttcaaaagt gtttttattt cattagagct tcctgaggat ttaatagggg
     6661 gagtatcaaa tatcataaat gcactaatat tggctatatt atctccagtc attctactgt
     6721 caaataaaaa acaatgtgca tttataattt ttttaattat attgtttgtt tataatgcca
     6781 ttttttataa taacatctat atgtttggtt ttataattat cgcatgcatg ttgatactaa
     6841 gtaacggaat atattggagg gatattatca gatacttgtc actggcccat tttttggtgt
     6901 ttctttttat cattccttta gttttttttg cagaaaaata ctcttttatt gatgatagat
     6961 ttggagttag atatacattt ggcttccata atccgaatac atttagtcaa tatttaattt
     7021 ctttatatgt agtgttcttg ttgttatttg ttgaagttat caaaaaaaaa tctctgcaat
     7081 ttttgactgt tataatattg actctattta tatatggggt gatatcctta actggttcaa
     7141 ggacgggaat gatattaact attattaccg gcttcggttt tttagcatgt acactgtcta
     7201 aaacgaatga taaaaaaaga agaaagttcg tatatttata catactaggt gcaggaatat
     7261 tatgttttat acaatattat cttgccttaa catataatca ttcagagttc tcaaaaaaca
     7321 taaatacaat tctttctggt agaatatggt tttcttcaca attaactagc caattatggc
     7381 ctgtcccata tttccatggt gtgaatatta atgattattt accaattgat ttcttctacg
     7441 tggcttattt ttacaatctt gggattttta ttggttcttg gtttatatac ctctttataa
     7501 gaaaaatgtt tgtgcaaaca tatactcctg ttatggtaat tgcgctttgg ttatcgcttg
     7561 caataacggt cactgaaaat tattatgcaa ttccgttgta taatattagt ttatttattg
     7621 ttttttcctc aagatatgtg attaatgaaa atgaaaacag ctgtacttat ccaagctcac
     7681 aaaaatgaaa attatattag agaattggcg ttaaacaatc catcagttcg tttttatgta
     7741 catatggatg ccaagtatcc aaataaaatc caatggatta aaaatgaatg tattgataac
     7801 atctatttaa ttgaaaatcc agtttcggtt tactgggggg gaagtagtca gatttttgca
     7861 actctattat tgatgaagaa ggcatacagt gataagagaa ataagttttt tcatctcgta
     7921 agttcagaat gcgttccatt aaagtcattt gttgaaattg aaaacgaatg gagcatgaat
     7981 gaaaactgtc aatttattga gtcgcataga gataaaaata atgagtggag actaaaggtt
     8041 cgggtccctc actcaaatac gaagtatctt aggacttttt taggacgatg tgccaataaa
     8101 ctctttaaag taactacttt tttatgggat agtgtaggtt taaatcccga aaactatttt
     8161 tttggttccc aatggttttc ggtaactaga aattttgttg agcaagtaat agatgaagat
     8221 aatgaagatt tttttaaaac attccataat ataacttgtg cagatgagca tgcttttgct
     8281 atttttgcac gaacaaaata ttctaacccg aatgatattc aagataacaa taaaagattc
     8341 attaagtttt taggtaagtc aagtccagaa tatttaagtt tagatcaagt ggggcagatt
     8401 aagaatctta actcgtattg gttttgcagg aaggtgagat gtaatgattt attaaaaata
     8461 ataaggcagg agaagtgatt atgctttttt ctatcattat gccggcatat aattcacagg
     8521 caacaataaa agaaagtata gcatccgtgc ttaatcagac atatcaaaat tttgaattaa
     8581 ttataattaa tgataattca agtgacgcaa ctttatcgat aataacaaat ttctgtcatg
     8641 atagaaggat tcttgtatta aataatgagg aaaatatggg agtggcacac tctcgtaaca
     8701 gaggattgga aatggcatct ggagaaataa ttgcctttct ggatagtgac gatatatggt
     8761 accccaataa attggaagag caatataatt gttttctttc tggtcacaaa attgtctgct
     8821 cttattatga tgtaattgac tctgaagggg atattgttgg aacaagaaat gcacctacac
     8881 tcgttacatt tgaaaaaatg cttaaaagta attttatagg taacttgact ggtgcatatg
     8941 cctcctcttt ttttggaaaa tgttatcaga gaaatattgg gcatgaggat tatattatgt
     9001 ggttggaatt ggtaaaaaaa caacctgctt attgcattaa aaataaacta gccgcatacc
     9061 gaatttcaaa taaatctctt tcttctaata aaatgaaagt ggtaatttgg caatggaaaa
     9121 tatatcgtaa agtattaggt atgaatatta tcaagtcttt atattacttc ttgaattata
     9181 tatattttgc gataaaaaaa agaaactaat attcaataag ttttaatttt tttatcattc
     9241 aatttcagta ataattgtag ttatttgcgt atagttatac cctaaccgaa catacccgca
     9301 gacaacaccc cctgacagga gtaaacaatg tcaaagcaac agatcggcgt cgtcggtatg
     9361 gcagtgatgg ggcgcaacct tgcgctcaac atcgaaagcc gtggttatac cgtctctatt
     9421 ttcaaccgtt cccgtgagaa aacggaagaa gtgattgccg aaaatccagg caagaaactg
     9481 gttccttact atacggtgaa agagtttgtt gaatctctgg aaacgcctcg tcgcatcctg
     9541 ttaatggtga aagcaggtgc aggcacggat gctgctattg attccctcaa gccatacctc
     9601 gataaaggtg acatcatcat tgatggtggt aataccttct tccaggacac cattcgtcgt
     9661 aaccgtgagc tttctgccga aggctttaat tttatcggta ccggtgtttc cggtggtgaa
     9721 gaaggtgcgc tgaaaggtcc ttccattatg cctggtgggc agaaagaagc ctatgaactg
     9781 gttgcaccga tcctgaccaa aatcgccgca gtggctgaag acggtgagcc atgcgttacc
     9841 tatattggtg ccgatggtgc aggtcactat gtgaagatgg ttcacaacgg tattgaatac
     9901 ggcgatatgc agctgattgc tgaagcctat tctctgctta aaggtggctt gaacctttcc
     9961 aacgaagaac tggcgcagac ctttaccgag tggaataacg gtgaactgag cagctacctg
    10021 attgacatca ctaaagacat cttcactaaa aaagatgaag acggtaacta cctggttgat
    10081 gtgattctgg atgaagcggc taacaaaggt accggtaaat ggaccagcca gagcgcgctg
    10141 gatctcggtg aaccgctgtc gctgattacc gagtctgtgt ttgcacgtta tatctcttct
    10201 ctgaaagatc agcgtgttgc cgcatctaaa gttctctctg gcccgcaagc gcagccagca
    10261 ggcgataagg ctgagttaat cgaaaaagtt cgtcgtgcgc tgtatctggg caaaatcgtt
    10321 tcttacgctc agggcttctc tcagctgcgt gcggcgtctg aagagtacaa ctgggatctg
    10381 aactacggcg aaatcgcgaa gattttccgt gctggctgca tcatccgtgc gcagttcctg
    10441 cagaaaatca ccgatgcata tgccgaaaat ccgcagatcg ctaacctgct gctggccccg
    10501 tacttcaagc aaatcgccga tgactaccag caggcgctgc gcgatgtcgt tgcttatgca
    10561 gtacagaacg gtatcccggt tccgaccttc gccgctgcgg ttgcctatta tgacagctac
    10621 cgtgccgctg ttctgcctgc gaacctgatc caggcacagc gtgactattt cggtgcgcat
    10681 acttataagc gcattgataa agaaggtgtg ttccatactg aatggctgga ttaatctgat
    10741 ttaaatcaat taaccaacac aaggcccgga gaaaccctcc gggctttttt attatacaaa
    10801 gcggcaggtt atggcctttt tttataattt atgattaaaa aagcgatata atacagagcc
    10861 gcacagcagg atcgctgtct tgacagttca accgcatcag cgttaaaaat cccgcagtag
    10921 atgaagttgt ggtggtggac taatgaccac tctaaatgtt taaccggaag aagtcagagc
    10981 taatgaaaat aacaatttca ggaacaggtt atgttggtct ttcaaatggt attctgattg
    11041 cgcaaaacca cgaagtggtt gcactggata tcgttcaggc caaagtggac atgcttaaca
    11101 agaagcagtc accgattgtt gataaggaga ttgaagagta tctggcgact aaagatctca
    11161 atttccgcgc tacgacagat aagtatgacg cgtataaaaa tgccgattac gttattattg
    11221 ccacacctac cgattatgat ccgaaaacaa attattttaa tacctcaagc gtggaagcgg
    11281 tcattcgcga tgtgacagaa attaatccta atgctgtcat ggtgattaaa tctacaattc
    11341 cggtaggttt caccaaatca attaaagaac gtctgggtat cgataacctt attttctctc
    11401 cggaatttct ccgtgaaggt aaagcccttt acgataatct ccatccttca cgtattgtca
    11461 ttggtgagcg ttcagaacgc gcagaacgtt tcgctgcgtt attacaggaa ggagcgatta
    11521 agcaaaatat cccgaccctg tttaccgact ccactgaagc agaagcgatt aaactttttg
    11581 caaacaccta cctggcgatg cgcgtggcgt actttaacga actggatagc tacgcagaaa
    11641 gtttaggtct gaattcccgt caaataatcg aaggcgtttg tctcgaccca cgaattggca
    11701 accattacaa caatccgtcg tttggttatg gtggttattg tctgccgaaa gataccaagc
    11761 agttactggc gaactaccag tctgtgccga ataacctgat ttcggcaatt gtcgatgcta
    11821 accgcacacg taaagacttt attgccgatg ccattttgtc acgcaaaccg caagtggtgg
    11881 gtatttatcg tctgattatg aagagcgggt cagataactt ccgtgcgtct tccattcagg
    11941 ggattatgaa acgtatcaag gcgaaaggcg ttgaagtgat catctacgaa ccggtgatga
    12001 aagaagattc attcttcaac tctcgcctgg aacgtgatct cgccaccttc aaacaacaag
    12061 ccgacgtcat tatctccaac cgtatggcag aagagcttaa ggatgtggca gacaaagtct
    12121 acacccgcga tctttttggc agcgactaac atcctgttat cagggcgatt ttcgccctga
    12181 ttctcttatg ttccctttgt aataattcat tatttttatc atttatccta tagcattcat
    12241 ggcgattatc gctaaactat ggcgacttaa aattatttcg tccgttaggg taatgatgag
    12301 agtagaaaat aataatgttt ctgggcaaaa ccatgacccg gaacagattg atttgattga
    12361 tttactggtg cagttgtggc gtggcaagat gacaattatc atctctgtca ttgttgctat
    12421 tgccctggct atagggtatc tggccgttgc caaagaaaag tggacttcca cagctatcgt
    12481 tactcaacct gatgtggggc aaatcgctgg ttataccaac gcgatgaatg tgatttacgg
    12541 atcggctgta ccgaaagtct ccgacattca ggcttcactt atcggacgtt acagcactgc
    12601 attttcagca ttagcggaaa cgctggataa ccaggaagaa gcagagaagc tcaccattga
    12661 gcctaccgtc aaaaatcagt cattaccttt agcggtgtct tatgttggtg aaacacctga
    12721 aggtgcgcag aaacagctgg cgaagtatat ccagcaagtt gacgaccagg taaatgaaga
    12781 actggaaaaa gacctgaaag acaacatcgc gctgcgtatg aaaaacttgc aggattctct
    12841 gaaaactcag gaagtagttg cgcaggagca gaaagatctg cgtatccgtc agattcagga
    12901 agcgttgcag tatgcgaatc aggcgcaggt gacaaagcca caggttcagc agactgaaga
    12961 tgtgacgcaa gatacgttgt tccttttggg gagcgaagcg ctggagtcga tgattaagca
    13021 cgaggcgact cgtccgttgg tgttctcatc aaactactat cagactcgtc aaaacctgct
    13081 ggatatcgac aatcttgacg ttgataaact tgatattcat gcttaccgct atgtaatgaa
    13141 accgacgtta cctattcgtc gcgatagccc gaaaaaggca attaccttga ttctggcggt
    13201 gctgctgggc ggcatggttg gcgcggggat tgtgttgggg cgtaacgctc tgcgtaatta
    13261 caacgcgaag taatcttttc ggttttaaag aaaatgggca gggtggtgac accttgcccg
    13321 tttttttgcc ggatgcgaca acaatatcgc atccgcttac cccgcaactc actgatgccg
    13381 tttacgcagg ttctcaatca ccgtcgttaa gtccagatcc tgatcctgca acagcactag
    13441 caggtgatac atcaagtcag atgcctcgtt ggtcagctca aagcggtcat gtacagtcgc
    13501 ggccagtgcg gtttccacgc cttcttcgcc cactttctgc gc
//
