LOCUS       AB811623               19664 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli DNA, O-antigen biosynthetic locus, strain: U12-41.
ACCESSION   AB811623
VERSION     AB811623.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 19664)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..19664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="U12-41"
                     /db_xref="taxon:562"
                     /note="serogroup:O49"
     gene            <1..859
                     /gene="wcaM"
     CDS             <1..859
                     /gene="wcaM"
                     /codon_start=2
                     /transl_table=11
                     /protein_id="BAQ00983.1"
                     /translation="NITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKIN
                     WGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNIT
                     PDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFRVNDI
                     QLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHFFMRNINVMQESS
                     VGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRINHHIVNVEKI
                     NFRLPELRE"
     gene            1022..1927
                     /gene="galF"
     CDS             1022..1927
                     /gene="galF"
                     /note="O049_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ00984.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            2300..3385
                     /gene="rmlB"
     CDS             2300..3385
                     /gene="rmlB"
                     /note="O049_02"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAQ00985.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNRESLA
                     DVSDSERYVFEHADICDAPAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELLLFTETTS
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQLWIEQNYEGRQ"
     gene            3385..4284
                     /gene="rmlD"
     CDS             3385..4284
                     /gene="rmlD"
                     /note="O049_03"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="BAQ00986.1"
                     /translation="MNILLFGKRGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVAS
                     GTTTWYDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMFNELFTTTAI"
     gene            4342..5220
                     /gene="rmlA"
     CDS             4342..5220
                     /gene="rmlA"
                     /note="O049_04"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAQ00987.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKNAYGQYLLKMIKGY"
     gene            5225..5764
                     /gene="rmlC"
     CDS             5225..5764
                     /gene="rmlC"
                     /note="O049_05"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="BAQ00988.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKCLEVI
                     LSANNKKQLWIPEGFAHGFLTLQDDTEIIYKTTNYYSPEYERAIIWNDEELSIKWPSK
                     PDIISQKDSLASAFSSIKY"
     gene            5775..6890
                     /gene="vioA"
     CDS             5775..6890
                     /gene="vioA"
                     /note="O049_06"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-xylo-4-hexulose aminotransferase"
                     /protein_id="BAQ00989.1"
                     /translation="MNKKIPVTQPFLPELNEFVPYLQQIWDNKWLTNNGPFHMELEEE
                     LCKYLGVKHISLFTNATIALVTALQALRISGEVITTPYSFVATSHSILWNNLKPVFVD
                     IDSETFNIDPDKIESAITSNTTAIMPVHCYGNPCDVEKIQKIADDYGLKVIYDAAHAF
                     GIKYKNNSLLNYGDLSILSFHATKVFNTFEGGAIICKDEKTKLRIDKLKNFGFVDEVT
                     VTAPGINGKMSEVNAAFGLLQLKYVNDAILKRKAIHDYYCQSLNEVTGLSIPEFSPNA
                     TRNYSYFPVLINEDFSVSRDKLHEILKYNNILSRRYFYPLISNMPMYRGLPSALCHNL
                     RNANSLSSKVLCLPIYSELTIEECDKIINLIKKVNER"
     CDS             6895..7548
                     /note="O049_07"
                     /gene="wcdL"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="BAQ00990.1"
                     /translation="MKSIIGIYGAGGFGREVLPIIRQQVGLKSDVVFIVDDIKEKVVN
                     ETKVVTFDEFCSLKDMDKYYTVAIADSKVRMEIDIKCIREGIKPISIYSQNSVIMDDV
                     LIGDGAILSPFTCITSNVRIGKSFHANLYSYIAHDCVIGDYVTLAPGVKCNGNVIIED
                     HAYVGTGAIIRPGTKTKPLVIGSHSIIGMGAVVTKTVPSGKTVFGNPAKILGNKRES"
     CDS             7550..7966
                     /note="O049_08"
                     /gene="wcdM"
                     /codon_start=1
                     /transl_table=11
                     /product="putative dehydratase"
                     /protein_id="BAQ00991.1"
                     /translation="MDPMQNLYIGMTAQYSQTVSDSDIKAFSGLSGDKNPVHMSDEYA
                     QKTMFKKRIAHGFMSASYFSAIFGTKLPGEGCVYLSQTLKFIKPVYIGDTVTAIVTLT
                     KINSRSRRLTFDTICVVKNETVISGVAEIYLPKISE"
     gene            8010..9512
                     /gene="wzx"
     CDS             8010..9512
                     /gene="wzx"
                     /note="O049_09"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ00992.1"
                     /translation="MLRRNVVFNYLGQFYISLMGICIFPLYLRYIGEEAYGLIGFFVL
                     LQTWMLLFDLGMSPTLSRQVAITNANNDFDFLRKLMRSLESVFFFIALIIMLLMAIFC
                     NDIAQKWLAVKNLNIDEVAVCIAIMGGVVSLRWFTSLYKSGINGYEKQAWLNVVNIFI
                     VTLRLPASLLLFIYFSKSLIIYFVYQLIISIVELFIYNRKMYRCLPKRLNDNHKIYLI
                     SWDVLKSVLPFAAGTAYTAGIWVLLTQLDKLLLSKVLTLSNFGFFSLVATLVGGILML
                     SSPISNAILPRITVLVSQGKIEQVVGLYTRCTRFVCCVIFPITMVMICYPYQVIYGWT
                     GNDVTAKWAMNVLPLYSLGNALLAVIGFQYYLQYAYGKLKLHIIYNTVLAIVSIPAVS
                     FFAIRYAAIGTGIVWIAINSITLLFWTYIVHNKFLKGVHFRWLFQDVLFPALISAMII
                     FIISNNIDTTSQSRIENILFVSLVSFTTIIISLFVSFFTDIMSFFKGMVK"
     CDS             9509..10741
                     /note="O049_10"
                     /gene="wcdN"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00993.1"
                     /translation="MKNVLFINSGFELGGIETFLVRAAKNLSKDVKYTLLIMSDATNK
                     DLLDKFSIYGDVIFLSDLLLFNAGKYAILRTLLPLNRQKVRTLLSHIDIIHASCSFSL
                     ILMRKLSCILNKGILESVGVYHSREFLWGEKKRLMRKTQLSMFKKIPANNVLFMNEYT
                     VKLYSDIFKVNYYNALPIGIDIGIYSECIPNKNSGRIVSIGRLVDFKTYNYHMIDYLS
                     TLSSERRNMLCFEIYGDGPESVALKKLAKQRNVKVKFGGRLEYKDMPSILDGASLFIG
                     SGTAIVEAAAAGVPCIIGIESIDEPLTYGFLTETVGLSFQEMGLNYPLKKYEMAMSEF
                     YELSEEDYLILSDKHRRRAKDFSLDNMKSIILDYYENLSDNTTRNKPCIMYALSTMFW
                     MILNKMKINNERNSMYDF"
     gene            10731..11885
                     /gene="wzy"
     CDS             10731..11885
                     /gene="wzy"
                     /note="O049_11"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ00994.1"
                     /translation="MISEKKNMRMSSFKAPLIFCLLAAFLPLGSTIGVAFSWVIFPAI
                     AIILTFLVSRFFYRSGVYFLVLSIVLLVLNYGIVLQYVSFAERLEFNMLLAIIGCFYM
                     SVVFFSYKFNFSDLSKAINIVLLISIILFFIQFLSYYLFNYRIDYSLATGGIGTRNDY
                     GSLYRASGIFNEPAEYSSAITVLVVSSYLINRKLSLLNIIALISTMLSFSFVGIIQSF
                     FVIYIVLFRNLCRKPVYIFLIIGIVILVWISFFDLFIDRYEAFINGSDGSNNTKLDTL
                     NFFLTHTEYLLGGAGLIGYDPETMPLFMQGLYDLTFFGSCISIFGIVIGSFIAIAVTV
                     YIIYNYTISDMTLIFICLLKINVMIYAVYWFFIILIIILPNYIQKTKVHV"
     CDS             11900..12721
                     /note="O049_12"
                     /gene="wbsF"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00995.1"
                     /translation="MPTIGRAEEIEKFISSVNKNNASVQLLIADQNEVGFLEPKKLYE
                     CAKNQNIDMHYFHSEQKGLSRNRNFLLSKLDAHEGIIAFPDDDCLYYEDTLDLVMSFF
                     ENNPSVDVLLGCIYDREYDRYLFKKWPRKSVVVNYLNVYFMSSSITIFVRKPNQQKFD
                     QALGAGAVYGSCEDPDYLYSLLKKGCKIVYEPSIQVWHPAPDYHQISLSKVYSYAKGF
                     GYFIRKDIDVYKSILLVLLISKKLFQLIFRFKHFPIKYFRAYFSGLISGLLRKQT"
     CDS             12718..13617
                     /note="O049_13"
                     /gene="wfgK"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ00996.1"
                     /translation="MSLVKRVAVLLAAYNGVKWIEEQIQSILNQKSIDVTIFISVDKS
                     TDGTEALVKKISHKYSNVKYLPFGFKFGGAAKNFYRLIKEVDFELFDFVSLSDQDDIW
                     YPNKLITAVNKINTGYDFYSSNVVAFWKNGKRIIINKAQKQLPYDYFFEAAGPGCTYV
                     FKSFQAIKLKEFIISNYKSVSEIALHDWLIYAFARNYNYKWYIDEWPSMDYRQHENNQ
                     VGANVTIYGVIKRVRLVRQKWYRNEIIKIAKLLQVSNNHFISKCLQERYLSNFYMIMN
                     MHKIRRRRRDRVMLFLLCVINWF"
     CDS             13700..14719
                     /note="O049_14"
                     /gene="gne"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-glucose 4-epimerase"
                     /protein_id="BAQ00997.1"
                     /translation="MTILITGGAGYIGSHTVLALLCNEYDVIVLDNECNSSIESLSRV
                     KSITGKEFLYVNGDVRDRGKLRTIFKQHEITDIIHFAGLKSVGESIKAPLQYYNNNVM
                     GAIVLLEEMISFNIPHIIFSSSATVYGEPTEIPVTERSKIGGTTNPYGTSKLMVEQIL
                     EDVTRTNPEFRATILRYFNPVGAHPSGHIGEDPNGTPNNLVPYVCQVAIGKYKQVSIY
                     GNDYPTKDGTGVRDFIHVMDLADGHVAAVQHRNEGKNHKIYNLGTGVGHSVLDILKTF
                     KRVASINIPYSFNNRRPGDIAECWSDPTKALNELGWKAKYDLDEMVRDAWNWQRKNPN
                     GYSKP"
     gene            14806..16212
                     /gene="gnd"
     CDS             14806..16212
                     /gene="gnd"
                     /note="O049_15"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ00998.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            16462..17628
                     /gene="ugd"
     CDS             16462..17628
                     /gene="ugd"
                     /note="O049_16"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ00999.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            17718..18752
                     /gene="wzz"
     CDS             17718..18752
                     /gene="wzz"
                     /note="O049_17"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01000.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGS
                     AVPKVSDIQTSLIGRYSTAFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETA
                     EGAQKQLAQYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSSNYYQT
                     RQNLLDIESLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(18848..19459)
                     /gene="hisI"
     CDS             complement(18848..19459)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01001.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     5882 a   3465 c   4232 g   6085 t
ORIGIN
        1 caacatcacc aattgtaagt tcagcgactt acaaggcgac gccattgaat ggaacgtggc
       61 aattaacgac cgtgatattt tgatatctga ccatgtcatc gagcgcatca actgtaccaa
      121 cggcaaaatc aactggggca tcggcatagg ccttgcggga agcacttatg ataacaacta
      181 cccggaagac caggcagtga aaaactttgt cgtggcgaat atcacgggat cggattgtcg
      241 gcagttgata catgttgaaa atggtaaaca ttttgttatt cgtaatatca aagcccgcaa
      301 tatcacgccg gatttcagta agaaagcggg cattgataac gcgacagtcg ctatttacgg
      361 ttgtgacaat ttcgtgattg ataatattga aatgattaat agcgccggga tgttaatcgg
      421 ctatggggta attaaaggca aatatctctc gataccgcaa aatttccggg tgaatgatat
      481 tcaactggat aatactcatc ttgcttataa attgcgcggc attcaaatct ccgccgggaa
      541 tgctgtttcc tttgtggcgc taactaacat tgagatgaag cgtgcgtcgc tggagttaca
      601 caacaaaccg caacattttt ttatgcgaaa tatcaatgtg atgcaggaat cctcagttgg
      661 acccgcattg agcatgaact ttgacatgcg caaagacgtt cgtggtgttt ttatggcgaa
      721 aaaagaaaca ctgctgtctc ttgcaaatgt tcatgcggtg aatgaaaaag gacaaagctc
      781 cgtcgatatc gacaggatta atcaccatat tgttaatgtg gagaagatta actttagatt
      841 gccggaactg agggagtaga tttgcgacca ttcctggaaa aatggagtca tacttaggaa
      901 caatgctact gcaatccaca acgaagcggc gtaacatcac aagtaattca gtaatcaatt
      961 cagggtaatt gatgctggcg aaaaaaatcg aacaagctat aattcagcaa ccattttaca
     1021 ggtggatgaa ataatgacga atttaaaagc agttattcct gtagcgggtc tcgggatgca
     1081 tatgttgcct gccactaagg cgatacccaa agagatgcta ccaatcgtcg acaagccaat
     1141 gattcagtac attgttgacg agattgtggc tgcagggatc aaagaaatcc tcctggtaac
     1201 tcacgcgtcc aagaacgcgg tcgaaaacca cttcgacacc tcttatgagt tagaatcact
     1261 ccttgagcag cgcgtgaagc gtcaactgct ggcggaagta cagtccatct gtccgccggg
     1321 cgtgaccatt atgaacgtgc gtcagggcga acctttaggt ttaggccact ccattttgtg
     1381 tgcgcgacct gccattggtg acaacccatt tgtcgtggta ctgccagacg ttgtgatcga
     1441 cgatgccagc gccgacccgc tacgttacaa ccttgctgcc atgattgcac gtttcaacga
     1501 aacgggccgc agccaggtgc tggcaaaacg tatgccgggt gacctctctg aatactccgt
     1561 catccagact aaagagccgc tggaccgtga gggtaaagtc agccgcattg ttgaatttat
     1621 cgaaaaaccg gatcagccgc agacgctgga ctcagacatc atggccgtag gtcgctatgt
     1681 gctttctgcc gatatttggc cggaactgga acgtactcag cctggtgcat ggggacgtat
     1741 tcagctgact gatgctattg ccgagctggc gaaaaaacaa tccgttgatg caatgctgat
     1801 gaccggcgac agttacgact gcggcaaaaa aatgggctat atgcaggcgt ttgtgaagta
     1861 tggcctacgc aacctgaaag aaggggcgaa gttccgtaaa ggtattgaga agctgttaag
     1921 cgaataatga aaatctgacc ggatgtaacg gttgataaga aaattataac ggcagtgaaa
     1981 attcgcagca aaagtaattt gttgcgaatc ttcctgccgt tgttttatat aaaccatcag
     2041 aataacaacg agttagcagt agggttttat tcaaagtttt ccaggatttt ccttgtttcc
     2101 agagcggatt ggtaagacaa ttagcgtttg aatttttcgg gtttagcgcg agtgggtaac
     2161 gctcgtcaca tcataggcat gcatgcagtg ctctggtagc tgtaaagcca ggggcggtag
     2221 cgtgcattaa tacctctatt aatcaaactg agagccgctt atttcacagc atgctctgaa
     2281 gtaatatgga ataaattaag tgaaaatact tgttactggt ggcgcaggat ttattggttc
     2341 agctgtagtt cgtcacatta taaataatac gcaggatagt gttgttaatg tcgataaatt
     2401 aacgtacgcc ggaaaccggg aatcacttgc tgatgtttct gattctgaac gctatgtttt
     2461 tgaacatgcg gatatttgcg atgcacctgc aatggcacgg atttttgctc agcatcagcc
     2521 ggatgcagtg atgcacctgg ctgctgaaag ccatgttgac cgttcaatta caggccctgc
     2581 ggcatttatt gaaaccaata ttgttggtac ttatgtcctt ttggaagccg ctcgcaatta
     2641 ctggtctgct cttgatagcg acaagaaaaa tagcttccgt tttcatcata tttctactga
     2701 cgaagtctat ggtgatttgc ctcatccaga tgaagtaaat aataacgaag aattactctt
     2761 atttactgag acgacatctt atgcgccaag cagcccttat tccgcatcaa aagcatccag
     2821 cgatcattta gtccgtgcgt ggaaacgtac ctatggttta ccgaccattg tgactaactg
     2881 ttcgaataac tacggtcctt atcactttcc ggaaaaattg attccactag taattcttaa
     2941 tgctctggaa ggtaaggcat tgcctattta tggtaaaggg gatcaaattc gcgactggct
     3001 gtatgttgaa gatcatgcac gtgcgttata taccgtcgta accgaaggta aagcgggtga
     3061 aacttataac attggtggac acaacgaaaa gaaaaacatc gatgtagtgc tcactatttg
     3121 tgatttgttg gatgagattg taccgaaaga gaaatcttac cgcgagcaaa ttacttatgt
     3181 tgccgatcgt ccgggacacg atcgccgtta tgccattgat gctgagaaga ttggtcgcga
     3241 attgggatgg aaaccacagg aaacgtttga gagcgggatt cgtaaaacgg tggaatggta
     3301 cctgtccaat acaaaatggg ttgataatgt gaaaagtggt gcctatcaat tgtggattga
     3361 acagaactat gagggccgcc agtaatgaat atcctccttt ttggcaaaag agggcaggta
     3421 ggttgggaac tacagcgtgc tctggcacct ttgggtaatt tgattgctct tgatgttcac
     3481 tccactgatt attgtggtga ttttagtaat cctgaaggtg tggctgaaac cgtcaaaaaa
     3541 attcgccctg atgttattgt taatgctgcg gctcatactg cagtagataa ggctgagtca
     3601 gaacccgaat ttgcacaatt actcaatgcg accagcgttg aatcaattgc aaaagcggct
     3661 aatgaagttg gggcctgggt aattcattac tcaactgact acgtatttcc gggaaccggt
     3721 gaaataccat ggctggagac ggatgcaacc gcaccgctaa atgtttacgg tgaaaccaag
     3781 ttagccggag aaaaagcgtt acaggaacat tgcgcgaagc atcttatttt ccgtaccagc
     3841 tgggtatacg caggtaaagg aaataacttc gccaaaacga tgttgcgtct ggcaaaagag
     3901 cgtgaagaat tagcggttat taacgatcag tttggtgcac caacgggtgc tgagctgctg
     3961 gctgattgta cggcacatgc tattcgtgtt gcactgaaaa aaccagaagt cgcaggcttg
     4021 taccatttgg tagccagtgg taccacaacc tggtacgatt atgctgcgct ggtttttgaa
     4081 gaggcgcgca aagcaggcat tccccttgca ctcaacaagc tcaacgcagt accaacaact
     4141 gcctatccta caccagctcg tcgtccacat aactctcgcc ttaatacaga aaaatttcag
     4201 cagaactttg cgcttgtctt gcctgactgg caggttggcg tgaaacgaat gttcaacgaa
     4261 ttatttacga ctacagcaat ttaatagttt ttgcatcttg ttcgtgatgg tggagcaaga
     4321 tgaattaaaa ggaatgatga aatgaaaacg cgtaaaggta ttattttagc gggtggttct
     4381 ggtacacgtc tttatcctgt gactatggct gtcagtaaac agctgttacc gatttacgat
     4441 aaaccgatga tctattaccc gctctctaca ctgatgttgg cgggtattcg cgatattctg
     4501 attatcagta cgccacagga tactcctcgt tttcaacaac tgctgggtga cgggagccag
     4561 tgggggctaa atcttcagta caaagtgcaa ccgagtccgg atggtcttgc gcaggcattt
     4621 atcatcggtg aagagtttat cggtggtgat gattgtgctt tggttcttgg tgataatatc
     4681 ttctacggtc atgatctgcc gaagttaatg gatgtcgctg ttaacaaaga aagtggtgca
     4741 acggtatttg cctatcacgt taatgatcct gaacgctacg gtgttgttga gtttgataaa
     4801 aacggtacgg caatcagcct ggaagaaaaa ccgctacaac caaaaagtaa ttatgcggta
     4861 accgggcttt atttctatga taacgacgtt gtcgaaatgg cgaaaaacct taagccttct
     4921 gcccgtggtg aactggaaat taccgacatt aaccgtattt atatggaaca ggggcgttta
     4981 tccgttgcca tgatggggcg tggttatgca tggctggata cggggacaca tcagagtctt
     5041 attgaagcaa gcaacttcat tgccaccatt gaagagcgcc agggactaaa ggtttcctgc
     5101 ccggaagaaa ttgcttaccg taaagggttt attgatgcag agcaggtgaa agcattagct
     5161 gagccgctga aaaaaaatgc ttatggtcag tatctgctga aaatgattaa aggttattaa
     5221 taaaatgaac gtaattaaaa cagagattcc agacgtatta attttcgaac cgaaagtttt
     5281 tggtgatgag cgtggtttct tttttgagag ctttaaccag aaggtttttg aggaagctgt
     5341 aggtcgcaaa gttgaatttg ttcaggataa ccattcgaag tctagtaaag gtgttttacg
     5401 cgggctgcat tatcagttgg aaccttatgc acaagggaaa ttggtgcgct gcgttgttgg
     5461 tgaggttttt gatgttgcgg ttgatattcg taaatcgtca cctacttttg gtaagtgctt
     5521 agaagttata ttatctgcaa ataataaaaa acaattatgg atccctgaag gatttgcgca
     5581 tggatttcta accttacaag atgatacaga aataatatat aaaaccacaa attactattc
     5641 accagaatat gaaagagcca ttatttggaa tgatgaagag ctttcaatta agtggcctag
     5701 taaaccggat atcatatctc aaaaagatag tttagcctca gcattttcat ctattaaata
     5761 ttgaagagaa ataaatgaat aagaaaatac cagttacaca accatttctt cctgaactaa
     5821 atgagtttgt cccttacttg caacagatat gggataataa atggttaaca aataatggcc
     5881 ccttccacat ggaactggag gaagaactat gtaaatattt aggggtaaaa catatatcat
     5941 tatttacaaa cgcaacaata gcattggtta cagctcttca agctttacga atctcaggag
     6001 aagtgatcac tacaccttat tcttttgttg cgactagtca ttctatatta tggaataatc
     6061 tcaaaccagt tttcgttgat atagatagtg aaacttttaa tattgatcct gacaaaatag
     6121 agtcagccat aacttctaat actacagcaa taatgccagt tcattgctac ggtaatccat
     6181 gtgatgttga gaaaattcaa aaaatagctg atgattatgg gttaaaggtg atttatgatg
     6241 cggcccatgc cttcggtatt aaatacaaaa ataacagctt gttgaattat ggtgatttgt
     6301 caattttaag tttccatgca acaaaggtgt ttaatacttt tgaaggagga gcaataattt
     6361 gtaaagatga aaaaactaag cttaggattg ataaattaaa gaattttgga tttgtggatg
     6421 aggtaactgt tactgccccc ggcataaatg ggaaaatgag cgaggttaac gcagcgtttg
     6481 gattattgca gttaaaatat gtcaatgatg cgattcttaa acgaaaagca atacacgatt
     6541 attactgcca atctttaaat gaagtgacgg gcttgtcgat acctgagttt tcgccaaacg
     6601 ctacaaggaa ctattcgtat tttccagttc ttattaatga ggatttttca gtatctaggg
     6661 ataaacttca tgagatattg aagtataata atatactctc tcggcgatat ttttaccctt
     6721 taattagcaa tatgccgatg tatagaggat taccttctgc tctttgtcat aatcttagaa
     6781 atgcaaattc tctctcttcg aaagttcttt gtttacctat ttatagcgaa ttaacaatag
     6841 aagagtgtga taagattatt aatctcataa agaaagttaa cgaaaggtaa ttatatgaaa
     6901 agcattattg gtatttatgg tgctggtggt ttcggtagag aggtgctacc tattataaga
     6961 caacaggttg gattaaaatc agatgtggtt tttatcgttg atgatatcaa agaaaaggta
     7021 gtcaatgaaa caaaggttgt aacatttgat gagttttgtt cgctaaaaga tatggataaa
     7081 tattatactg ttgcaatagc agacagtaaa gtgagaatgg agatagatat aaaatgcatc
     7141 cgagagggaa ttaagcccat ttctatttat tctcaaaaca gtgtgataat ggatgatgta
     7201 ctgattggtg atggtgcgat tttatctcct tttacttgta taacatcaaa tgtaagaatc
     7261 gggaaatctt ttcatgccaa tttatatagt tatatagcgc atgattgtgt tattggagac
     7321 tatgtcactt tagcaccagg tgtaaaatgt aacggtaatg ttattataga ggatcatgct
     7381 tatgtcggaa ctggggcaat aattcgacct gggaccaaaa caaaaccact cgtaattgga
     7441 agccattcta ttattggaat gggggcggtt gtcactaaaa cagtaccttc aggaaaaaca
     7501 gtatttggga atccggcaaa aatattaggc aataagagag aatcataata tggatcctat
     7561 gcaaaattta tacatcggga tgacggcaca gtattcccag actgtaagtg atagtgatat
     7621 aaaagcgttc tctgggctaa gtggtgataa aaaccctgtt catatgagcg atgaatatgc
     7681 gcagaaaacc atgtttaaga aacgaattgc tcatggtttt atgtcagcaa gttatttttc
     7741 tgcaatcttt ggcacaaaat taccagggga gggatgtgta tatctttctc agacactaaa
     7801 atttataaag ccagtttata ttggtgatac agttaccgct attgtaacct tgacgaaaat
     7861 aaattcacgc tctcggcgat tgacatttga tacaatctgt gttgtcaaga atgaaactgt
     7921 gatatccggt gttgcagaga tatatttacc taaaataagt gagtagatga taattagatt
     7981 ttaaaaagta aaacattaag cgaaaataca tgttaagaag aaatgttgtt tttaattatt
     8041 tagggcaatt ttacatatcc cttatgggga tatgtatttt tcctctatat ttaagatata
     8101 ttggagagga agcgtatggt ttaattggtt tttttgtttt gcttcaaaca tggatgcttt
     8161 tatttgattt aggcatgtca ccaacacttt ctaggcaagt ggccataact aatgctaata
     8221 atgattttga ttttttaaga aaattgatga gaagtctcga atcggtattt ttctttatag
     8281 cattaattat tatgctgtta atggccattt tttgtaatga tatagcccaa aaatggttgg
     8341 ccgttaagaa cttaaatatt gatgaagtgg cagtatgcat tgcaataatg ggaggggttg
     8401 tttctttacg atggtttact agtctctata aaagcggaat aaatggttat gaaaagcaag
     8461 catggctcaa tgtagttaat atttttattg taacattaag attgcctgca tctttgcttc
     8521 tatttattta cttttcaaaa tccctaataa tatattttgt atatcagctg attatatcta
     8581 ttgttgaatt gtttatttat aacaggaaaa tgtatagatg tctgccaaaa cgattaaatg
     8641 ataatcataa gatatattta atatcatggg atgttttaaa aagtgtttta ccttttgctg
     8701 caggaactgc gtatactgca ggaatatggg tgttattaac gcaattggat aagcttttac
     8761 tttcgaaagt gttaacgctc agcaactttg gttttttttc attggttgca acgttggttg
     8821 gaggtatatt aatgctttct tctcccatca gtaatgccat tttaccgcga ataacggttc
     8881 ttgtgtcgca aggtaaaatt gagcaagtag ttggtttgta tacacgttgt acaagatttg
     8941 tttgttgcgt aatatttcct attacgatgg ttatgatatg ttatccttat caggttattt
     9001 atggatggac aggaaatgac gttaccgcaa aatgggcgat gaacgtattg cccctgtatt
     9061 ctttgggtaa tgctctttta gcagttattg gatttcagta ttacttacaa tatgcgtatg
     9121 gtaaattaaa attacacatt atatataaca ctgtattggc gattgtgtcg ataccagcag
     9181 taagtttttt tgctattaga tatgcagcaa taggcacggg aattgtttgg atcgcaatta
     9241 atagtattac attattgttt tggacgtata ttgtgcataa taaatttctt aaaggagttc
     9301 atttcagatg gttatttcaa gacgttttat tccccgcatt gatttcagca atgatcatat
     9361 ttattatttc aaataatata gacacaacat cacagagtag aattgaaaat atactttttg
     9421 tatctttagt tagttttaca acaataatta tatctctttt tgtttctttt tttaccgata
     9481 ttatgagctt ttttaaagga atggttaaat gaaaaatgtt ttatttatta atagtggttt
     9541 tgaacttggc ggaattgaaa cattccttgt tagagcagct aaaaatttat ctaaagatgt
     9601 aaagtataca ctcttaataa tgagtgatgc tacgaacaaa gatctccttg acaagttctc
     9661 tatatatggc gatgttattt ttttgagtga tttgctatta tttaatgcag gtaaatatgc
     9721 aatactaaga acccttttac ctcttaatcg acaaaaagtt agaactttgc tctctcacat
     9781 tgatataatt catgcctcgt gctctttctc attaattcta atgcgaaaac taagttgtat
     9841 tcttaataaa ggaatactag agtcagttgg tgtctatcat tccagagagt ttttatgggg
     9901 ggaaaaaaaa agattaatga ggaaaactca actttcaatg tttaaaaaaa ttcctgcaaa
     9961 taacgtactt ttcatgaatg aatatacagt taaattatat tcagatatat ttaaggtgaa
    10021 ttattataat gctttaccaa ttggtatcga tattggtatt tattctgaat gtataccgaa
    10081 taaaaatagc ggaagaattg tatccatcgg aagattggtc gattttaaaa catataatta
    10141 tcatatgata gattatttat ctacattatc tagtgaaaga agaaatatgt tatgttttga
    10201 aatatatgga gatggcccgg aatcagttgc actgaaaaaa ttagcaaaac aaaggaatgt
    10261 gaaagtaaag tttggagggc gattagagta taaagatatg ccatccattt tagatggtgc
    10321 aagtttattt ataggttcag gtactgccat cgttgaagcg gcggcagctg gtgtaccttg
    10381 tatcataggg attgaatcaa ttgatgagcc tctgacatat ggttttctta cagaaacagt
    10441 ggggctttcg ttccaagaaa tgggactgaa ttatcccctt aaaaaatatg aaatggcgat
    10501 gagtgaattt tatgaacttt cagaagaaga ttacttaata ttatcagata agcatagaag
    10561 aagagccaaa gatttttcat tagataatat gaagtcaatt atacttgatt attatgaaaa
    10621 tttatctgat aacacaacta gaaataaacc atgtataatg tatgcattaa gcacaatgtt
    10681 ttggatgata ttaaataaaa tgaaaattaa taatgagaga aattcaatgt atgatttctg
    10741 aaaagaaaaa catgagaatg tctagtttta aagctccttt aatattttgt cttcttgcag
    10801 catttttacc tctaggtagt acaattggag ttgccttttc ttgggtgata tttccagcta
    10861 ttgccataat attaactttt ttagtttcac gtttttttta tagatctggt gtttattttt
    10921 tagttctgtc gatagtactt ctagttttaa attatggcat agttttacaa tatgtttcat
    10981 ttgcagagcg tttagagttt aatatgctgc ttgcaattat tggttgtttt tatatgtcag
    11041 tggttttttt ttcatataaa ttcaattttt ctgacttgtc aaaagcaatt aatatagtat
    11101 tactaattag tattatttta ttttttattc aatttttatc atattatttg tttaattata
    11161 ggatcgatta tagtttagct acaggtggta ttggtactcg taacgattat ggctcattgt
    11221 atagagcatc tggtatcttt aatgaaccag ctgagtattc atcagcaata actgttcttg
    11281 ttgtttcaag ttatttaata aacagaaaat tatcactatt aaatatcata gcccttataa
    11341 gcacaatgtt atctttttca tttgtgggaa ttattcagtc tttctttgtc atatatattg
    11401 ttttattccg aaatctttgt aggaaaccgg tatatatttt tttaattatc ggtattgtta
    11461 ttttggtttg gatttctttt tttgatttat ttattgacag atatgaggca tttattaatg
    11521 gaagtgatgg tagcaataat acaaaactag atacgttaaa tttcttttta acgcatacag
    11581 aatacctttt ggggggggct ggcttaattg gatacgatcc agaaacgatg ccattattta
    11641 tgcaaggatt atatgatcta acattctttg gctcgtgtat cagtattttt ggtattgtta
    11701 ttggttcatt tattgccata gcggtgactg tttatattat atataattat actatttctg
    11761 atatgacact aatttttata tgtttgttaa aaattaatgt aatgatatat gcagtatact
    11821 ggttttttat aatattaatt ataattctgc ctaactacat acaaaaaacg aaggtacatg
    11881 tgtgaaaatt catttattaa tgcctacgat agggcgggct gaagaaattg aaaaattcat
    11941 atcatcagta aataagaata atgcatcagt gcaattgctt atcgctgatc aaaatgaagt
    12001 aggatttctt gaacctaaaa aattatatga atgcgctaag aatcagaata ttgacatgca
    12061 ttattttcat agtgaacaaa aaggattgtc tcgtaacaga aactttttac tttcaaaatt
    12121 agatgcccat gaaggtatta tagcattccc tgatgatgac tgcctatatt atgaagatac
    12181 tttagatttg gtaatgtcat tttttgaaaa taatccgagt gttgatgttt tattgggatg
    12241 tatatacgat agggaatatg acagatattt atttaaaaag tggccgagaa aaagtgttgt
    12301 ggttaattat ttaaatgtat attttatgtc ttcctctata acaatttttg ttcggaaacc
    12361 gaatcagcaa aaattcgatc aagcacttgg agcaggggct gtatacgggt catgtgaaga
    12421 tccagattat ctttatagtt tactaaaaaa aggatgcaaa atagtatatg aaccctccat
    12481 ccaagtttgg catcctgctc ctgattatca tcaaatatcc ttgagtaagg tatattccta
    12541 tgctaaagga ttcggatatt ttatacgtaa agatattgac gtttataaat ctatattgtt
    12601 agtattgtta atttctaaaa aactgtttca attgatattt agatttaaac attttccgat
    12661 aaaatatttc agagcgtatt tttcaggttt aattagtgga ctattgagaa agcaaacatg
    12721 agtcttgtca agagagttgc tgttttatta gcagcatata atggagttaa gtggattgaa
    12781 gaacaaattc aatcaatact taatcagaaa agtattgatg tgacaatatt tataagcgtt
    12841 gataaatcta ctgacggtac agaggctttg gttaaaaaaa ttagccacaa atatagtaat
    12901 gtaaaatatt tacccttcgg ttttaaattc ggaggtgcag ctaaaaattt ttataggctc
    12961 ataaaagagg ttgattttga gctatttgac tttgtatctc tgtcagatca agatgatatt
    13021 tggtacccta ataaattgat tacagctgtt aataagatca acaccggtta cgatttttac
    13081 tcaagtaacg ttgtggcttt ttggaaaaat ggtaaaagaa taattattaa caaagcgcaa
    13141 aaacaactac cgtatgatta tttttttgaa gccgctggtc ctggatgtac atatgttttt
    13201 aaatcttttc aagctatcaa gctgaaagaa tttattattt ctaattataa atctgtatca
    13261 gaaattgcac ttcatgattg gttaatttat gcctttgcac gaaattacaa ctataaatgg
    13321 tatattgatg aatggccatc catggattat cgacaacatg aaaataacca ggttggtgct
    13381 aatgtaacca tatatggtgt aataaaacgc gtaagactag taagacaaaa gtggtaccga
    13441 aatgaaatta taaaaatagc aaaattatta caggtatcaa ataatcattt tatctcaaaa
    13501 tgtttacaag aaagatactt gagtaacttt tacatgatca tgaatatgca taaaatccga
    13561 cgtagaagaa gagatagggt tatgttattc ctgctgtgtg tgattaattg gttttaattt
    13621 catacataat tcgattgtat tcagctatat ttatggtgtt ttccattgga ttccatttaa
    13681 taataaatag gaggtaacgt tgacaattct gattactggc ggagcaggat acattggttc
    13741 tcatacagtg ttggctcttt tgtgcaatga atatgatgtc attgttctgg ataacgaatg
    13801 caattcttct attgaatcgt tatctcgtgt taagtcaatc accggaaagg aatttttata
    13861 tgttaatggt gatgtaagag atcgtggaaa gttacgtact atttttaaac aacatgaaat
    13921 tactgatatt atacattttg cagggttgaa atctgtcggt gaatctatta aagcaccttt
    13981 acaatactac aataataatg ttatgggtgc aattgttctt ttagaggaaa tgattagttt
    14041 taatattcct catattatat ttagttcttc ggcaacagtt tatggcgaac ctactgaaat
    14101 acctgtaact gaaagatcga aaataggagg aacaactaat ccatatggta cttcaaaact
    14161 tatggttgag cagatcttag aggacgttac tcgtactaat cctgaattta gagcaactat
    14221 actacgttat ttcaaccctg ttggtgctca tccttcaggt cacataggtg aagatcctaa
    14281 cggtactcct aacaatctag ttccttatgt ttgccaagtt gctatcggaa aatataaaca
    14341 ggtttcaata tatggcaatg attatccaac taaagatggt acaggtgttc gtgatttcat
    14401 tcatgtcatg gatcttgctg acggtcatgt tgcagcagta caacaccgga atgaagggaa
    14461 gaatcataaa atatataacc ttggaacagg tgtaggacac tcagttttag atattttgaa
    14521 aacctttaaa cgtgttgcgt caattaatat tccatattct tttaataata gacgtccggg
    14581 ggatattgct gagtgttggt ccgatcctac caaggcatta aatgaattag ggtggaaagc
    14641 aaaatatgat ttagatgaaa tggttcgaga tgcttggaat tggcagagaa aaaatcctaa
    14701 tggttatagt aaaccttaat taatttctat ctatgtttat attctctgcg gtcaaaaaat
    14761 tttaatggtt tcattacaaa ttaattcccc tgacaggagt aaacaatgtc aaagcaacag
    14821 atcggcgtcg tcggtatggc agtgatgggg cgcaaccttg cgctcaacat cgaaagccgt
    14881 ggttataccg tctctatttt caaccgttcc cgtgaaaaga cggaagaagt gattgccgaa
    14941 aatccgggca agaagctggt tccttactat acggtgaaag agtttgttga atctctggaa
    15001 acgcctcgtc gcatcctgtt aatggtgaaa gcaggtgctg gcacggatgc tgctattgat
    15061 tccctcaagc catacctcga taaaggtgac atcatcattg atggtggtaa taccttcttc
    15121 caggacacca ttcgccgtaa ccgtgagctt tctgccgaag gctttaactt catcggtacc
    15181 ggtgtttccg gcggtgaaga gggcgcgctg aaaggtcctt ccattatgcc tggtggccag
    15241 aaagaagcct atgaactggt tgctccgatc ctgaccaaaa tcgccgccgt tgctgaagat
    15301 ggggagccat gtgttaccta tattggtgcc gatggcgcgg gtcactatgt aaaaatggtt
    15361 cacaacggta ttgaatacgg tgatatgcag ctgattgctg aagcctattc tctcctgaaa
    15421 ggcggcctga atctctctaa cgaagaactg gcgcagacct ttaccgagtg gaataacggt
    15481 gaactgagca gctacctgat cgacatcacc aaagatatct tcactaaaaa agatgaagac
    15541 ggtaactacc tggttgatgt gatcctggat gaagcggcta acaaaggtac cggtaaatgg
    15601 accagccaga gcgcgctgga tctcggcgaa ccgctgtcgc tgattaccga gtctgtgttt
    15661 gcacgttata tctcttctct gaaagagcag cgtgttgccg catctaaagt tctctctggc
    15721 ccgcaagcgc agccagctgg cgacaaaggt gagttcatcg aaaaagttcg tcgtgctctg
    15781 tatctgggca aaatcgtttc ttacgctcag ggcttctctc agctgcgtgc ggcgtctgaa
    15841 gagtacaact gggatctgaa ctacggcgaa atcgcgaaaa ttttccgggc tggctgcatc
    15901 atccgtgcgc agttcctgca gaaaatcacc gatgcttatg ccgaaaatcc gcagatcgct
    15961 aacctgctgc tggctccgta cttcaagcaa attgccgatg actaccagca ggcgctgcgt
    16021 gatgtcgttg cttatgcagt acagaacggt atcccggttc cgaccttcgc tgctgcggtt
    16081 gcctattacg atagctaccg tgccgctgtt ctgcctgcga acctgatcca agcacagcgt
    16141 gactatttcg gtgcgcatac ttataagcgc attgataaag aaggtgtgtt ccatactgaa
    16201 tggctggatt aatctgattt aaccaacaat aaaattgagg cccggcgtat attgcaccgg
    16261 gctttttttt tgccaattat cttatagact aaatttcact gcttaatatt aacttaataa
    16321 atatcagcta ttcttataaa gaaaatctga attgtttttc gttgcgttga ccatttgaca
    16381 acgtagcgtt aaaactttta gctcttatca ggatgctaaa aacatcatga ttcacagtta
    16441 agttaattct gagagcatga aatgaaaatc accatttccg gtactggcta tgtaggcttg
    16501 tcaaacgggc ttctaatcgc tcaaaatcat gaggttgtgg cattagatat tttaccgtca
    16561 cgcgttgcta tgctgaatga tcggatatct cctattgttg ataaggaaat tcagcagttt
    16621 ttgcaatcag ataaaataca ctttaatgcc acattagata aaaatgaagc ctaccgggat
    16681 gctgattatg tcatcatcgc cactccaacc gactatgatc ctaaaactaa ttatttcaat
    16741 acatccagtg tagaatcagt aattaaagac gtagttgaga taaatcctta tgcggttatg
    16801 gtcatcaaat caacggttcc cgttggtttt accgaagcga tgcataagaa atatcgtact
    16861 gaaaatatta ttttctcccc cgaattcctc cgcgaaggta aagctcttta cgataacctt
    16921 catccttcac gtattgtcat cggtgagcgt tcagaacgcg cagaacgttt tgctgcgttg
    16981 ttacaggaag gggcgattaa gcaaaatatc cctacattgt ttaccgactc cactgaagca
    17041 gaagcgatta aacttttcgc taatacctat ctggcgatgc gcgtagcata ctttaatgaa
    17101 ctggatagct atgcagaaag tttagggctg aatactcgcc agattatcga aggcgtttgt
    17161 ctcgatccgc gtattggcaa ccattacaac aatccttcgt ttggttatgg tggttattgt
    17221 ctgccgaaag ataccaagca gttactggcg aactaccagt ctgtgccgaa taacctgatt
    17281 tcggcaattg tcgatgctaa ccgcacgcgt aaagacttta ttgccgatgc cattttgtca
    17341 cgcaagccgc aagtggtggg tatttatcgt ctgattatga agagcgggtc agataacttc
    17401 cgtgcgtctt ccattcaggg gattatgaaa cgtatcaagg cgaaaggcgt tgaagtgatc
    17461 atctacgaac cggtgatgaa agaagactca ttcttcaact ctcgcctgga acgtgatctc
    17521 gccactttca aacaacaagc cgatgtcatt atttccaacc gtatggcaga agagcttaag
    17581 gatgtggcag acaaagtcta cacccgcgat ctctttggca gtgactaaca tcctgttatc
    17641 agggcgattt tcgccctgat tctcttatgt tccctttgta ataattcatt atttttatca
    17701 tttatcctat agcattcatg gcgattatcg ctaaactatg gcgacttaaa attatttcgt
    17761 ccgttagggt aatgatgaga gtagaaaata ataatgtttc tgggcaaaac catgacccgg
    17821 aacagattga tttgattgat ttactggtgc agttgtggcg cggcaagatg acaattatca
    17881 tctctgtcat tgttgctatt gccctggcta tagggtatct ggccgttgcc aaagaaaagt
    17941 ggacttccac agctatcgtt actcaacctg atgtggggca aatcgctggt tataccaacg
    18001 cgatgaatgt gatctacgga tcggctgtac cgaaagtctc cgacattcag acttcactta
    18061 tcggacgtta cagcactgca ttctcagcat tagcggaaac gttggataat caggaagaac
    18121 ctgaaaaact gaccattgag cctactgtta aaaatcagtc cttaccttta gcggtgtctt
    18181 atgttggtga aacagctgaa ggtgcgcaga aacagctggc gcaatatatc cagcaagttg
    18241 acgaccaggt aaacgaagaa ctggaaaaag acctgaaaga caacatcgcg cttcgtatga
    18301 aaaacttgca ggattcgctg aagacccaag aagtggttgc gcaggagcag aaagatctgc
    18361 gtatccgtca gattcaggaa gcgttgcagt atgcgaatca ggcgcaagtg acaaaaccac
    18421 aggttcagca gactgaagat gtgacgcaag atacgttgtt ccttttgggg agcgaagcgc
    18481 tggagtcgat gattaagcac gaggcgactc gtccgttggt gttctcatca aactactatc
    18541 agacacgtca aaacctgctg gatatcgaaa gcttaaaggt tgatgatctt gatattcatg
    18601 cttaccgcta tgtaatgaaa ccgacgttac ctattcgtcg cgatagtccg aaaaaggcaa
    18661 tcaccttgat tctggcggtg ctgctgggcg gcatggttgg cgcggggatt gtcttggggc
    18721 gtaacgctct gcgtaattac aacgcgaagt aatctttgcg gttttaaaga aaatgggcag
    18781 ggtggtgaca ccttgcccat ttttttgccg gatgcgacaa caatatcgca tccgcttacc
    18841 ccgcaactca ctgatgccgt ttacgcaggt tctcaattac cgtcgttaaa tccagatcct
    18901 gatcctgcaa cagcaccagc aggtgataca tcaagtcaga cgcctcgttg gtgagttcaa
    18961 agcggtcatg taccgttgct gccagtgcgg tttccacgcc ttcttcaccc actttctgcg
    19021 caatgcgctt ggtgccgctg gcatacagtt tggcggtgta ggaggtttcc gggtcggcag
    19081 atttgcgttc ggcgagcagt tgttccagtt gatacaggaa cagccactgg tgagcggtgt
    19141 cgccgaagca gctgctggtg cctttgtggc aggtcgggcc gatgggattc gccagcaaca
    19201 gtaacgtgtc gttgtcgcag tccggcgtaa tatttactac gttgaggaaa ttgcccgacg
    19261 tttcgccttt ggtccacagt cgctgtttag tgcgcgagaa gaaggtgact ttgccgcttt
    19321 cgatggtttt atctaaggct tccgggttca tatagcccag cattaacact tcaccggata
    19381 ccgcgtgctg cacaatcacc ggcataagtc cgtcggtttt ttcccagtcc agttcgcgac
    19441 gttgttgttc tgttaacata tcctgatctc cacgccctgt gttgccaggt acgcttttaa
    19501 ttcaccaata ttgattattt gtttgtggaa tacggaagcc gccagcgcgc cgtcaacgtc
    19561 ggcatcgcgg aaggcttcga ggaagtgttc catggtgccc gcgccaccgg aggcaatcag
    19621 cggaacgtgg caaacttcac gcactttttt cagctgttcg aggt
//
