LOCUS       AB811624               16181 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli DNA, O-antigen biosynthetic locus, strain: U18-41.
ACCESSION   AB811624
VERSION     AB811624.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 16181)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..16181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="U18-41"
                     /db_xref="taxon:562"
                     /note="serogroup:O50"
     gene            <1..793
                     /gene="galF"
     CDS             <1..793
                     /gene="galF"
                     /note="O050_01"
                     /codon_start=2
                     /transl_table=11
                     /protein_id="BAQ01002.1"
                     /translation="PMIQYIVDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLE
                     LRVKRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVID
                     DASADPLRYNLAAMIARFNETGRSQVLAKRMPGDLSEYSVIQTKDPLDREGKVSRIVE
                     FIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQAVD
                     AMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            1166..2251
                     /gene="rmlB"
     CDS             1166..2251
                     /gene="rmlB"
                     /note="O050_02"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAQ01003.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     EVSDSERYSFEHADICDAEAMARIFSQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSGLDDEKKKNFRFHHISTDEVYGDLPHPDEVNNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            2251..3150
                     /gene="rmlD"
     CDS             2251..3150
                     /gene="rmlD"
                     /note="O050_03"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="BAQ01004.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTNYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPDFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGSGDAPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3209..4084
                     /gene="rmlA"
     CDS             3209..4084
                     /gene="rmlA"
                     /note="O050_04"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAQ01005.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEDFIGG
                     DDCALVLGDNIFYGHDLPKLMEAAVNKESGATVFAYHVNDPERYGVVEFDNNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     IKNQYGQYLLKMISE"
     CDS             4099..4512
                     /note="O050_05"
                     /gene="fdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="putative dTDP-6-deoxy-3,4-keto-hexulose
                     isomerase"
                     /protein_id="BAQ01006.1"
                     /translation="MMDIKLISLQKHGDERGALIALEEQRNIPFEVKRIYYILETLNG
                     VRRGFHAHKVTRQLAIVVKGACKFHLDNGKETKQVELNDPTIALLIEPYIWHEMYDFS
                     DDCVLLVIADDFYKESDYIRNYDDFIRRVNSIENS"
     CDS             4499..4966
                     /note="O050_06"
                     /gene="fdtC"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="BAQ01007.1"
                     /translation="MRIHKLSDVQTTSIGDGTTIWQFVVILKGAVIGNNCNICANTLI
                     ENNVVIGNNVTVKSGVYIWDGVKIEDNVFIGPCVAFTNDKYPRSKVYPDEFLQTIIRK
                     GASIGANATILPGIEIGEKAIVGAGSVVTKNVPPCAIVVGNPARFIKWVEDNE"
     CDS             4959..6065
                     /note="O050_07"
                     /gene="fdtB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminotransferase"
                     /protein_id="BAQ01008.1"
                     /translation="MNKIDFLDLFAINQRQHKELVSAFSRVLDSGWYIMGEELEQFEK
                     EFAEYCGVKYCIGVANGLDALILVLRAWKELGYLEDGDEVLVPANTYIASILAITENK
                     LVPVLVEPDIETYNINPALIENYITEKTKAILPVRLYGLLCNMPEISAIARKYNLLIL
                     EDCAQAHGAIRDGRKAGAWGDAAGFSFYPGKNLGALGDAGAVTTNNAELSSTIKALRN
                     YGSHKKYENIYQGLNSRLDELQAALLRVKIHTLPEDTAIRQRIAEKYIREIKNPAITL
                     PVYEGQGAHVWHLFVVRIANREKFQSYLLEKGIKTLIHYPLPPHKQQAYQNMSSLSLP
                     ITEQIHDEVISLPISPVMSEDDVNYVIKMVNDYK"
     gene            6065..7327
                     /gene="wzx"
     CDS             6065..7327
                     /gene="wzx"
                     /note="O050_08"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01009.1"
                     /translation="MKKFLQVTILSAIYTFIKMIAGFIIGKVVAIYTGPSGVAMLGQV
                     QSLITIVAGTTSAPVSTGLVRYTAENWQEGQEACAPWWRACLRVTLFLFLLIIPVVII
                     LSKNISELLFSDGQYTWLIIFACCILPFSIINTLIASVLNGQQFYKQYILVGMFSVFI
                     STMFMILLIVAYNLKGALIATAINSAIAGLVLVLFCLNKSWFRFKYWWGKTDKDKIIK
                     IIHYTLMALVSVISMPTALMCIRKILIAKTGWEDAGQWQAVWKISEVYLGVVTIALST
                     YFLPRLTIIKTSFLIKKEVNSTILYIISITSFMALSIYLFRDLVITVLFTEQFRSARE
                     LFLLQLIGDVIKIAGFLYAYPLQSQGHTKLFISSEVIFSMLFIITTYIFVVNYGVHGA
                     NISYVITYSLYFVFAFVFTNFINVRRNN"
     CDS             7344..8393
                     /note="O050_09"
                     /gene="wekP"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01010.1"
                     /translation="MKIIIPVLGFGRAGGERVLSKLATELMNYGHDVSFVVPDNRTNP
                     YYATTAKIVTSKSSQNRVKILRIIKNYYNLWRKCIELNPDAVVASFHLTAYLVALLPI
                     TRRKKYYYIQAYEVNFFDNIIWKLIAGLTYYLPLKKILNSPNLLPHKHDDFIGVVPAG
                     VDLNVFYPKPSNRLLNGHTSIGIIGRKEKHKGTSEIISVLCSLENKAGIIINIAIYLE
                     EVDKQRLIAAGFQVNFFPITSDLELASFYRSNDIMIAVGLIEDGAFHYPCAESMACGC
                     LVISNYAPLTETNSVLKLVKFDACKLGEAINLCLNLDLEEKSKEIQSNISVLNKYDWK
                     IVGETFNSLLLDANK"
     gene            8414..8971
                     /gene="rmlC"
     CDS             8414..8971
                     /gene="rmlC"
                     /note="O050_10"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="BAQ01011.1"
                     /translation="MNIVKTDIPDLIVLEPKVFSDERGFFMESYNQIEFEKAIGRHVN
                     FVQDNHSKSSKGVLRGLHYQLAPYAQAKLVRCVVGQVFDVAVDLRKNSPTFKKWFGIT
                     LSAENKRQLWIPEGFAHGFLVTSDEAEFIYKTTNYYAPGHQQAIIYNDPILNIDWPFC
                     SSALSLSQKDQEAKLFSELLDSELF"
     gene            9040..10002
                     /gene="wzy"
     CDS             9040..10002
                     /gene="wzy"
                     /note="O050_11"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01012.1"
                     /translation="MTENSPKYKHDKSLISFIYLFFIFTLIVGFIIAITQFLGRSRDY
                     DNYIQIFSGKEGEGVLELFYRGLMLITTSYETIIFIILTCSFFIKARFLANYSRNFSG
                     LTLFFIYYASVALWVLDYTQFRNGLCISILMFSVYYLFINKPTYFYFSVLCAIATHWS
                     ALPFLLLYPFVYSTKIRRLGYFCFSILVLIAISGEGKEIISFIRNFGVGQKIGNEAGV
                     NLINSLSLTAISWFIISYISSIGNERRNLRLFFCYGVMQYVTFSLFSLPVMAFRILEM
                     YFFLMLTIGVFIKQKKNYYFVFCKVLILLYLTYYYHMVFGVINV"
     CDS             9995..10882
                     /note="O050_12"
                     /gene="wekQ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01013.1"
                     /translation="MCKAKVLAIIVTYNPEIIRLTECINSLAPQVERIILVDNGSNNS
                     DLIKNISINNLEIILLSENKGIAFAQNHGVKKGLEAKEFDYLFFSDQDTCFPSDVIEK
                     LKSTFTKNNKKGKNVACASPFFKDHRSNYMHPSVSLNIFTSTKVICSEVDDDLYPSHV
                     IASGMLMSREAWRVVGPFCEKLFIDWVDTEWCWRALANNMIIVQTPSVIISHELGYGQ
                     KIFAGRSVTIHNSFRNFYKIRNAIYLMLHSNYSFKYRYHAFFHATKNVVFEILYSKEK
                     LNSLKVCFKAVRDGMFNNF"
     CDS             10913..11989
                     /note="O050_13"
                     /gene="wbuV"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01014.1"
                     /translation="MEENNMKTVAVVGTVGVPACYGGFESLVQNLIDYQSDGIQYQIF
                     CSSKKYDKKFKNYKNAELIYLPINANGVSSIIYDIMCLIICLFKRPDVVLILGVSGCL
                     FLPIYKLFSKSKIIVNIDGLEWRRNKWGTFAKKFLKISEAISIRIADIIISDNQAIAD
                     YVENKYKKKSVVIAYGGDHATNLSTPIDNDQKKEGYYLGLCRIEPENNIEMILNAFIN
                     TDKKIKFMGNWDNSEYGRQLKKYYSNYPNITLLEPNYNIEELYKLRKNCLAYIHGHSA
                     GGTNPSLVEAMHFNIPIFAFDCDFNRYTTNNLAHYFNDSEQLSLLAESLSFGNLKCRV
                     LDLKNYAEDMYNWRHIAAMYESIY"
     CDS             12168..12722
                     /note="O050_14; transposase of ISEc1"
                     /gene="ISAs1"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     CDS             13002..13304
                     /note="O050_15; transposase of ISEc1"
                     /gene="ISAs1"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     CDS             13565..14059
                     /note="O050_16; transposase of ISAs1 family"
                     /gene="ISAs1"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     CDS             14246..14599
                     /note="O050_17; transposase of ISAs1 family"
                     /gene="ISAs1"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
BASE COUNT     5042 a   2642 c   3375 g   5121 t
ORIGIN
        1 gccaatgatt cagtacattg ttgacgagat tgtggctgca gggatcaaag aaatcctcct
       61 ggtaactcat gcgtccaaga acgcagtcga aaatcacttc gatacatctt atgaattaga
      121 atctctcctt gaactgcgcg tgaagcgcca gcttctggcg gaagtacaat ctatctgccc
      181 gccaggtgtg accattatga acgtgcgtca gggggaacct ttaggtctgg gccactcgat
      241 tttgtgtgcc agaccagcca ttggcgataa cccgtttgtc gtggtgctgc cggatgttgt
      301 tatcgacgac gccagtgccg atccgctgcg ttacaatctg gccgcaatga ttgcgcgctt
      361 caatgaaact ggccgcagcc aggttctggc aaaacgcatg ccgggcgatc tctctgaata
      421 ctccgtcatc cagaccaaag atccgcttga tcgcgaaggc aaagtcagcc gcattgttga
      481 atttatcgaa aaaccggatc agccgcagac cctggactca gacatcatgg ctgtagggcg
      541 ttatgtgctt tctgccgata tttggcctga actggagcgt actcaacctg gagcatgggg
      601 acgtattcag ttgactgatg ccattgctga gttggcaaaa aaacaagcag ttgacgcaat
      661 gctgatgact ggggacagtt acgactgtgg taagaaaatg ggttatatgc aagcgtttgt
      721 gaagtatgga ctacgcaacc tgaaagaagg cgctaagttc cgcaaaggca ttgagaagtt
      781 gttaagcgag taacaaattc ctgacaggaa gtgtcagtca gtaagtaatt aaaaacggca
      841 gtgaagattc gtagcataag ttttgctcat tgaatctcac tgccgttgtt gtttttataa
      901 atgcaatgaa aacaggatgt tagcttgtaa tcatagttca aatttttcca ggattttcct
      961 tgtttctaca gctgtttggt aagacaatta gcgtttgaat ttttcggatt tggcacgagc
     1021 gggatacgct cgtcacatcg tagaaatgta cgcagtgctc tggtagctgt taagccaggg
     1081 gcggtagcgt gcattaatac ttctattaat caaatctcga gcagtctatt tcacagtatg
     1141 ctctctggct atatggaata aaaaagtgaa gatacttgtt actggtggcg caggctttat
     1201 tggttctgct gttgttcgtc acataataaa taatacgcaa gatagtgttg ttaatgtcga
     1261 taaattaaca tacgccggaa acctggaatc gctcgctgaa gtttctgatt ctgagcgtta
     1321 ttcatttgag catgcagata tctgcgatgc cgaagcgatg gcgcgtattt tttcacaaca
     1381 ccagccagat gcggtgatgc accttgcggc tgagagccac gttgaccgct caataactgg
     1441 cccggcggca ttcattgaaa ctaatattgt aggtacttac gtacttttag aagctgcgcg
     1501 caattattgg tctggtctgg atgatgaaaa gaaaaaaaac ttccgctttc atcatatttc
     1561 tactgatgaa gtatatggtg acttacccca tccggatgaa gtaaataata acgaagcgtt
     1621 accgctattt acggaaacga cagcctacgc gccaagtagc ccgtattctg cttctaaagc
     1681 ttccagcgat catttggttc gcgcatggaa acgtacgtat ggtttaccga ccattgtgac
     1741 taattgctcg aacaactatg gtccgtatca cttcccggaa aagcttattc cattggttat
     1801 tcttaatgca ctggaaggta aggcattacc tatttatggc aaaggggatc aaattcgcga
     1861 ctggttgtat gtagaggatc atgctcgtgc gttatatacc gtcgtaaccg aaggtaaagc
     1921 gggtgaaact tataacattg gcggacacaa cgaaaagaaa aacatcgatg ttgtgctgac
     1981 tatttgtgat ttgttggatg agattgtacc gaaagagaaa tcttatcgtg agcaaattac
     2041 ttatgttgct gatcgcccag ggcatgatcg ccgttatgca attgatgccg ataaaattag
     2101 ccgcgaattg ggctggaaac cacaggaaac gtttgagagc gggattcgca aaacggtgga
     2161 atggtatctg gctaatacaa attgggttga gaatgtgaaa agcggtgctt atcagtcatg
     2221 gatcgaacaa aactatgagg gccgtcagta atgaatatcc tgcttttcgg caaaacaggg
     2281 caggtgggtt gggaactgca gcgtgctctg gcgccgctgg gtaatctgat cgctcttgat
     2341 gttcactcca ctaattattg tggagatttc agcaaccccg aaggtgtggc agaaaccgtc
     2401 aaaaaaattc gtcctgacgt tattgttaat gctgctgctc acactgcagt agataaagca
     2461 gaatcagaac cggatttcgc acaattactt aacgcgacaa gcgtcgaagc gattgcaaaa
     2521 gctgctaatg aagtcggggc ctgggttata cactactcta ctgattatgt tttcccaggc
     2581 agtggtgacg cgccatggct ggaaacggat gcaacagcac cgctaaatgt ttacggtgaa
     2641 acaaaattag ctggggaaaa ggcattacaa gaacattgcg caaagcatct tattttccgt
     2701 accagctggg tatacgctgg taaaggaaat aactttgcta aaacgatgtt gcgtttggca
     2761 aaagaacgcg aagaactggc tgtgataaac gatcagtttg gcgcaccaac aggtgctgaa
     2821 ttgctggctg attgcaccgc tcatgccatt cgcgtggcat taaaaaaacc agaagtcgct
     2881 ggcttgtacc atctggtagc aagtggcaca acaacctggc acgattatgc tgcgctggtt
     2941 tttgaagagg cgcgcaaagc agggattaat cttgcactta acaaacttaa cgccgtgcca
     3001 acaacggcct atcccacacc agcccgtcga ccccataact ctcgcctcaa tacagaaaag
     3061 tttcagcaga actttgcgct tgtcttgcct gactggcagg tgggcgtgaa acgtatgctc
     3121 aacgaattat ttacgactac ggcaatttaa caaatttttg catctcgctc atgatgccag
     3181 agcgggatga attaaaagga atggtgaaat gaaaacgcgt aaaggtatta ttctggctgg
     3241 tggttccggc actcgtcttt atcctgtgac gatggcagtg agtaaacaat tgctgccgat
     3301 ttatgataag ccgatgattt attatccgct ttcaacgctt atgttagcgg gtattcgcga
     3361 tattcttatt attagtacgc cacaggatac accgcgtttc caacaattat tgggggacgg
     3421 gagccagtgg ggtcttaatc tacagtataa agtacaaccg agtccggatg gcctggcgca
     3481 agcgtttatt attggcgaag actttattgg tggtgatgat tgtgcactcg tacttggcga
     3541 taatatcttc tatggacacg acttgccgaa attgatggaa gctgctgtta acaaagaaag
     3601 cggtgcaacg gtatttgctt atcacgttaa tgatcctgaa cgctatggtg tcgtggagtt
     3661 tgataataac ggtacggcaa ttagcctgga agaaaaaccg ctggagccaa aaagcaacta
     3721 tgcggttact gggctttatt tctatgacaa tgacgttgtg gaaatggcta aaaaccttaa
     3781 gccttctgcc cgtggcgaac tggaaattac cgatattaac cgtatttata tggaacaagg
     3841 acgtttgtct gtagccatga tggggcgtgg ctatgcatgg ttggatacag ggacgcatca
     3901 aagccttatt gaagcaagta acttcattgc aacaattgaa gagcgtcagg gattaaaggt
     3961 atcttgcccg gaagagattg cttaccgtaa agggtttatt gatgccgagc aggtgaaagt
     4021 attagccgaa ccgcttatca agaatcaata tggtcaatat ttgctgaaaa tgatcagcga
     4081 atagtatatg ggaactcaat gatggatatt aaattaatct ctttgcaaaa acatggggat
     4141 gagcgcggtg cattaattgc tcttgaagag caacgaaata tacctttcga agtcaaaaga
     4201 atatattaca tacttgagac tcttaatgga gtaagacgcg gatttcatgc gcacaaggtt
     4261 actcgtcagt tagctattgt agtcaaggga gcttgtaaat ttcatctgga taatggtaaa
     4321 gaaacaaagc aggtggaact taatgatcca acaattgcgt tgctgataga accctatata
     4381 tggcatgaaa tgtatgattt tagtgatgat tgtgtgctgc ttgtaattgc ggatgatttc
     4441 tataaagagt ctgattatat ccgcaattat gatgatttta ttagaagagt aaattcaatt
     4501 gagaattcat aagctaagtg acgtccagac aacatcaatt ggtgatggaa caactatctg
     4561 gcagtttgtt gtgatactaa aaggtgctgt aattggtaat aattgcaaca tctgtgcaaa
     4621 taccttaatt gaaaataacg ttgtaattgg taacaatgtc acagtcaaaa gcggtgtgta
     4681 tatttgggat ggcgttaaaa tagaggataa tgtttttatt ggtccttgtg tagcatttac
     4741 aaatgataag tatcctcgct ctaaagtcta tcctgatgaa tttttgcaaa caataatacg
     4801 caaaggagca tcaataggtg ctaacgcaac catcctgcca ggaattgaaa ttggtgaaaa
     4861 agcaatcgtt ggtgcgggga gtgttgtaac caaaaatgta ccgccatgcg caatagtagt
     4921 aggtaatcca gctcgattta ttaaatgggt agaggataat gaataaaatt gattttttag
     4981 atctttttgc aattaaccag cgacagcaca aagaattagt ctctgcgttt agtagggtgc
     5041 tagattctgg ttggtatatc atgggcgaag aacttgagca gttcgagaaa gagttcgcag
     5101 aatactgtgg agttaagtat tgcattggtg tagcaaatgg ccttgatgcg ttgatactag
     5161 tattgagggc atggaaagaa cttggctatc ttgaagacgg tgacgaggta ttagtaccgg
     5221 caaatacata tattgcttct attcttgcta taacagagaa caaacttgtt cctgttcttg
     5281 ttgaaccaga tatagaaact tataatatta atcctgcttt aattgaaaat tacattacgg
     5341 aaaaaactaa agcaatatta ccggttcgct tatatggtct attgtgcaat atgccagaaa
     5401 ttagtgcaat cgccagaaaa tataatctgt tgattcttga agattgtgca caagcacatg
     5461 gtgcaatacg tgatggtcgc aaagctggag cttgggggga tgctgcagga tttagttttt
     5521 atccaggaaa aaaccttgga gctttggggg atgcgggagc tgttactaca aataatgcag
     5581 aattatcctc aactataaaa gctttgcgaa attatgggtc acataagaaa tatgaaaata
     5641 tttatcaggg attgaatagt cgattggatg aactgcaagc agccttattg cgtgtaaaaa
     5701 tccatacatt accggaagat actgcgattc ggcaaaggat tgctgaaaaa tatattcgtg
     5761 aaataaaaaa ccctgcgatt acgttaccag tgtacgaagg ccaaggtgcg cacgtttggc
     5821 atttatttgt agtaagaatc gctaatcgtg aaaaattcca gtcatactta ttagagaagg
     5881 gtatcaaaac cttaattcac tatccattac caccccataa gcagcaagca tatcaaaata
     5941 tgtctagcct tagccttcca attactgagc aaattcatga tgaagtcatt tctttaccta
     6001 taagtccggt aatgagtgaa gatgatgtca attatgtaat caaaatggtc aatgattaca
     6061 agtaatgaaa aaatttcttc aggtaactat attatccgct atctatacat tcattaaaat
     6121 gattgcgggt tttatcatcg gtaaggtagt agcaatttat acagggccat caggggtagc
     6181 aatgcttggc caagtgcaaa gtttaatcac aatagttgca ggtactacct ctgcacctgt
     6241 aagcactggc cttgttcgat atactgcgga aaattggcaa gaaggacaag aagcatgcgc
     6301 gccatggtgg cgcgcatgct taagggttac tctgttttta ttcttgctta ttattcccgt
     6361 tgttattata ttgtcgaaaa atattagtga gttacttttt agcgatggac aatacacatg
     6421 gttaatcatt ttcgcatgtt gtatattgcc attctccatt ataaatacat tgatcgcttc
     6481 agttttaaat ggtcaacaat tttataagca atatatattg gttgggatgt tttctgtatt
     6541 catttctact atgtttatga ttttgttgat tgtagcttat aatcttaaag gtgcattgat
     6601 tgccacagct ataaatagtg ctattgctgg tcttgtattg gttttatttt gtctcaataa
     6661 atcttggttt agatttaaat attggtgggg taaaacggat aaagacaaaa ttataaaaat
     6721 tattcattat actctgatgg ctctggtttc tgttatctcc atgcctacag cattgatgtg
     6781 tattagaaaa atattgattg ctaaaactgg ttgggaggat gcagggcaat ggcaggccgt
     6841 atggaagata tctgaggttt atcttggtgt tgtgacaatt gctttgtcaa catatttctt
     6901 accaagattg acaattataa aaacaagttt ccttataaaa aaagaagtaa atagtactat
     6961 attatacata atatctatta cttcattcat ggcgttgagt atctatttat tccgcgattt
     7021 ggtaataaca gttttattta ctgaacagtt tcgctcagct cgtgaattat ttttattaca
     7081 acttataggg gatgtaataa aaattgctgg gtttctttat gcataccctc ttcaaagtca
     7141 ggggcatact aaactattca tcagttcaga agtgattttt tctatgctct ttatcattac
     7201 cacctatatt tttgttgtaa attatggagt acatggtgct aacataagtt atgtcattac
     7261 atatagttta tattttgtgt ttgcatttgt gtttactaat tttattaatg ttagaagaaa
     7321 taattaaaaa cagaggttga attttgaaaa taattatacc tgtcttagga tttggcaggg
     7381 ctggtggtga aagagttctt tctaagctgg caactgaatt gatgaattat ggacatgatg
     7441 taagttttgt tgttccagat aatagaacta atccatatta tgctaccaca gcaaaaattg
     7501 tcacgagtaa atctagtcaa aaccgtgtaa aaatattgag aatcattaaa aattactata
     7561 atctgtggcg taaatgcata gagttaaatc ctgatgctgt agttgctagt tttcatttga
     7621 ctgcctatct tgtcgcatta ttaccaatca cccgtcgtaa gaaatattat tatattcagg
     7681 cgtatgaagt taattttttt gataatataa tatggaaatt aatagcgggt ttaacatatt
     7741 atttaccgct taaaaaaata ctaaatagtc ctaatttgct tcctcataaa catgatgatt
     7801 ttataggagt agttcctgca ggagtagatt taaacgtttt ctatccgaaa ccatcaaata
     7861 ggttattaaa tggtcacaca tcaataggga ttattggtag aaaagagaag cacaaaggaa
     7921 ctagcgaaat tatttcagta ttgtgttcac tggaaaataa agctggaatt ataatcaata
     7981 ttgcgatcta tcttgaagaa gttgataagc agcgtttaat cgctgccggg tttcaggtta
     8041 atttttttcc gattacttct gatttagaat tggcatcctt ttatcgaagc aatgacatca
     8101 tgattgctgt tgggttaatt gaagatggcg ctttccatta tccttgtgct gaatcaatgg
     8161 cttgtggttg tcttgttatt tcaaattatg cgccacttac tgaaactaac agtgtactta
     8221 aattagtcaa gtttgatgct tgcaaacttg gtgaagcaat taatctttgt ctcaatcttg
     8281 acctagaaga aaaaagcaaa gaaatccaat ctaatatttc tgtgttgaat aaatatgact
     8341 ggaaaattgt tggtgaaact ttcaatagtt tattgttaga tgcaaataaa tagtatacgt
     8401 tgatggggaa aatatgaata ttgttaaaac tgatattcca gatctgatcg ttcttgaacc
     8461 aaaagtgttt agtgatgaac gcggcttttt tatggagagt tataatcaga ttgaatttga
     8521 gaaggcaata ggaaggcacg taaattttgt tcaggataat cattcaaaat ctagtaaagg
     8581 cgtactacgt gggttgcatt atcaattagc accgtatgca caggctaaat tagttcgatg
     8641 tgttgtaggt caggtatttg atgttgctgt tgatcttaga aaaaattcac caacgttcaa
     8701 aaaatggttt ggaataaccc tttccgcaga aaataaacga caattatgga tacccgaagg
     8761 atttgctcat ggtttcttgg tgaccagtga tgaagctgag ttcatttata agacaactaa
     8821 ctactatgct cctggtcatc agcaagcaat tatttacaat gatcctattt taaacatcga
     8881 ttggcctttc tgcagtagtg ctctgtcatt atcacaaaaa gatcaagaag caaaattatt
     8941 ttcagaatta ttggacagtg aactgttcta ataaagtgtg ccaccttatc cgtctgaagg
     9001 ataggtggtt gcttatattt ttttgagtat gtttgtataa tgacagaaaa tagtccgaaa
     9061 tataaacacg ataaaagctt aataagtttt atctacttat tttttatatt tacacttatt
     9121 gtaggcttta ttatcgcaat tacccagttt ttggggcgaa gtagagacta tgataattat
     9181 atacagatct tttctggtaa agaaggggag ggggttcttg aattatttta tcgcggattg
     9241 atgttaataa cgaccagcta tgaaactatc atttttataa ttttaacatg ttcttttttt
     9301 ataaaggcaa ggtttctcgc taactattcg cgtaattttt caggcttgac cttattcttt
     9361 atttattatg caagcgttgc actttgggtt ttagattata ctcaattcag aaatggtcta
     9421 tgtatttcca ttttaatgtt ttccgtatac tatttattta taaataaacc gacttatttt
     9481 tatttctcgg tattatgtgc aattgcaact cattggtctg ctttgccttt tttgctttta
     9541 tatccttttg tctattcaac aaaaataaga cgccttggtt atttttgttt cagtattctt
     9601 gttttgattg cgatctcagg agaaggaaaa gagatcatat cttttataag aaattttgga
     9661 gtgggacaaa aaataggaaa tgaagctggt gtaaatttaa taaattcatt atcccttacc
     9721 gctatttcct ggtttattat tagttacata tcaagcattg gaaatgaaag gagaaattta
     9781 aggcttttct tttgttatgg tgtcatgcaa tacgtgactt ttagcctttt ctctctacct
     9841 gttatggctt tccgtatttt ggaaatgtat tttttcctta tgctaaccat tggggtgttt
     9901 attaagcaaa aaaagaatta ttattttgtt ttttgcaaag tgttaatttt attgtatcta
     9961 acatactatt atcatatggt ctttggagtg attaatgtgt aaggctaagg tgttggctat
    10021 aattgttact tacaacccgg aaattattcg attgacggaa tgtattaact ctttagcccc
    10081 acaagttgag agaataattc ttgtagataa tggctcaaat aatagtgatt tgataaaaaa
    10141 tatcagtatt aataaccttg aaattatttt actttcggaa aacaaaggca ttgcatttgc
    10201 tcagaaccat ggtgttaaga agggcctgga agcaaaagag tttgactatt tatttttctc
    10261 agatcaggat acttgctttc ctagcgatgt tattgaaaaa cttaagagta catttacgaa
    10321 aaataataaa aaaggtaaaa atgttgcttg tgcttctcct ttttttaaag accatcgttc
    10381 aaattatatg catccgtcag tcagcctaaa tatttttacg agtacaaaag ttatatgtag
    10441 tgaagtagac gatgatcttt atccctcgca tgttattgct tctgggatgt taatgtctcg
    10501 tgaagcatgg cgcgtcgtcg gaccattttg tgaaaaactc tttatagact gggttgatac
    10561 agaatggtgt tggcgtgcat tagctaataa tatgattatt gttcagacac catcagtcat
    10621 catttctcat gaacttgggt atgggcagaa aatttttgct ggtcgatctg ttacaataca
    10681 taattctttc agaaattttt ataaaatacg caatgcaata tacttaatgc tgcattcaaa
    10741 ttatagcttc aagtatcgtt atcatgcttt ttttcatgcg acaaagaatg ttgtatttga
    10801 aattttatat tcgaaagaaa aattaaattc actgaaggtt tgttttaaag ctgtacgtga
    10861 tggtatgttc aataattttt aatacgaaaa tagttaggct caaggtgttt aaatggaaga
    10921 aaataatatg aagacggtcg ctgtagttgg cacagtgggt gttcctgctt gttatggtgg
    10981 gttcgaatca cttgttcaga atctaattga ttatcaatct gatggtatac aatatcagat
    11041 attttgctct tcaaaaaaat atgataaaaa atttaaaaat tataaaaatg cagaattaat
    11101 ctatttgccg ataaatgcca atggcgtctc tagcataatt tatgatatta tgtgtttaat
    11161 tatttgttta ttcaaaaggc cagatgttgt tttaatattg ggggtgtctg gttgtttatt
    11221 tctaccaatt tataaactat tttcaaaatc aaagattatt gtcaatattg atgggcttga
    11281 atggcgtaga aataaatggg gaacgtttgc taagaaattt cttaaaatat ctgaggcgat
    11341 atctattaga atagctgata ttatcatttc agataatcaa gcaatagctg attatgtgga
    11401 aaataagtac aagaaaaaaa gtgtagttat agcttatggc ggagatcatg ccactaatct
    11461 tagtacaccg atagacaatg atcaaaaaaa agaaggttat tatttggggc tttgtaggat
    11521 agagcctgag aataatatag aaatgattct gaatgccttc attaatacag ataaaaaaat
    11581 taaatttatg ggtaattggg ataacagcga gtatggacgc cagctaaaaa aatattattc
    11641 aaactatcca aatatcaccc tactagaacc taactataat attgaagagc tttataaact
    11701 aagaaaaaat tgtcttgcat acattcatgg acactcggct ggtggaacaa acccttcttt
    11761 agttgaagcg atgcatttta atattcctat ttttgctttc gattgtgact ttaatcgtta
    11821 cacaactaac aatttagctc attactttaa tgattctgaa caacttagct tattagcaga
    11881 aagtttgtct tttggaaatc ttaaatgtcg agtattagat ttaaaaaatt atgctgaaga
    11941 tatgtataac tggaggcata tagctgctat gtatgaatct atttattaaa cgcattaaca
    12001 ataatataat tgaccttata tagcagggaa agatcacgta acgctgcttt tttgtactaa
    12061 ataattcgca ttttatgttt aaaaattgag atatccctta ttacctaaag ctgtttttta
    12121 ttgcttatac atgatcaaat actccttaca taattaagga gaacaaaatg gaacttaaaa
    12181 aattgatgga acatatttct attatccccg attacagaca agcctggaaa gtggaacata
    12241 aattatcgga tattctactg ttgactattt gtgccgttat ttcgggtgca gaaggctggg
    12301 aagatataga ggattttggg gaaacacatc tcgatttttt gaagcaatat ggtgattttg
    12361 ataatggtat tcctgttcac gatactattg ccagagttgt atcctgtatc agtcctgcaa
    12421 agtttcacga gtgctttatt aactggatgc gtgactgcca ttcttcagat gataaagacg
    12481 tcatcgcaat tgatggaaaa acactccggc attcttatga caagagtcgc cgcaagggag
    12541 cgattcatgt cattagtgcg ttctcaacaa tgcacagtct ggtcatcgga cagatcagga
    12601 cggatgagaa atccaatgaa attacagcta tccctgaact tcttaacatg ctggatatta
    12661 aaggaaaaat catcacaact gatgcgatgg ggtgccagaa agatattgca gagaagatat
    12721 aaaaacaggg cggtgattat ttattcgctg taaaaggaaa tcaggggcgg cttaataaag
    12781 cctttgagga aaaatttcca ctgaaagaat taaataatcc agaacatgac agttacgcca
    12841 ttagtgaaaa gagtcacggc agagaagaaa tccgtcttca tattgtttgc gatgtccctg
    12901 atgaacttat tgatttcaca tttgaatgga aaggactgaa gaaattatac gttgcagtct
    12961 cctttcggtc cataatagca gaacaaaaga aagagccaga aatgacggtc agatattata
    13021 tcagttctgc tgatttaacc gcagagaagt tcgccacagc aatccgaaac cactggcacg
    13081 tggagaataa gctgcactgg cgtctggacg tggtaatgaa tgaagacgac tgcaaaataa
    13141 gaagaggaaa cgccgcagaa ttattttcag ggatacggca catcgctatt aatattttaa
    13201 cgaatgataa ggtattcaag gcagggttaa gacgtaagat gcgaaaagca gccatggaca
    13261 gaaactatct cgcgtcagtc cttgcgggga gcgggctttc gtaatcttgc cctgccttat
    13321 atagctatca gagtaaaaaa tgtaacgctt attattttgt taaataatac gcattatata
    13381 ttcaataatt aaaatgctct taattaaata aaactattta attaataata taaaatttca
    13441 ttatgttact taagagatga aaattataac ttaaaaaagt gatggaacat atttctatta
    13501 tccctgatta cagacaagcc tggaaagtgg aacataaatt gtcggatatt ctactgttga
    13561 ctatttgtgc cgttattttt ggtgcagaag gttgggaaat atagaggatt ttggggaaac
    13621 acatctcgtt tttttgaagc aatatggtga ttttgaacat ggtattcctg ctcacgatac
    13681 cattgccaga gttgtatcct gtatcagtcc tgcaaaattt cacgagtgct ttattaactg
    13741 gatgcgtgat tgttattctt catatgataa agacgtcatc gcaattgatg gaaaaacact
    13801 ccggcattct tatgacaaga gtcgccgcaa gggagcgatt catgtcatta gtgcgttctc
    13861 aacaatgcac agtctggtca tcggacagat caagacggat gagaaattca atgaggtcac
    13921 agctatccca gaacttctta acatatggat attaaaggaa aaattatcac aactgatgcg
    13981 atgggttgcc agaaagatat tgcagagaag atacaaaaac gggggaggtg attatttatt
    14041 cgctgtaaaa gtaaattagg ggcggcttaa taaagccatt gagggataat ccccgctgaa
    14101 agaattaaat aatccaaagc atggcagtta cgaaataagt gaacagagtc acggcagaga
    14161 agacatccgt cttcatattg tttgcgatgt tcctgataaa cttattgatt tcacgtttta
    14221 atggaaagga ctgataaaat tatgcgtggc agtctccttt cggtcaataa tagcagaaca
    14281 aaagaaggag cctgaaatga cggtcagata ttatatcagt tctgcgggtt taacagcaga
    14341 gaagtttgcg acagtgattc gaaatcactg gcacgtgaaa aataagctgc actggcgtca
    14401 gggcgtggta atgaatgaag accactgcaa aataagaaga ggaattgcag cagaattatt
    14461 ttcagggatc aggtatatcg gcattaatat tttgacgaaa cataaagtat tcaaggctgg
    14521 gttaagatgt aagatgagaa gagcagatat ggacagagac tacctcgcat cagtttttgc
    14581 ggggagctgg ctttcgtaat cttgccctgg tgatgcaatg ctgaattaat agcattaaag
    14641 attttaaata aaatggttta actcaacatg aactttataa ttattattag aatgattttg
    14701 cctttctgta tttttttgag tattttactt tactctggaa gggtggatag tgttttttta
    14761 ttttctattg aatacacatt tataagtgtg gcaatctttc tgataaagag aaagtttgta
    14821 agtaatattt tatgggtaat ttatctttcc attttaggtg tgcaggcagc gtcgctattt
    14881 agttcaggac aatatttgat gcctcttgcc ttgtcgaatg ctgcagaaat tgaatccttg
    14941 ggtattggtg aggtaattaa agtttttttt gtttttattt tgtttattat aatttcattc
    15001 attaccttgc cttcaggggc tctaaccgag cgtattaaat attataaatt caaccttctc
    15061 tgtatagtat tattgctcgt gtgttctttc ataagtgcgg gaccagtata tgctttttat
    15121 gaaacagcaa aattgttcta tttacagcaa acctttaagc ctagtaagaa gatcggagat
    15181 gcagggaaat tctttttaaa aacaagtgtt tttattaacc ctatagaaaa caactacagt
    15241 ttcaaaggcc gtaatgttat tgtaatattt gctgagggat tttcatctga aattatgggt
    15301 aatgccaaac caagagcatt ttcagttacg cctaatttag ataaattatc tactgaagca
    15361 ctaagtttca aaaattatta taaccataca gctgcaacat tccgaggatt aagaggtcag
    15421 ttaacttctg gttatcaatt tcgagatggt ttaactgacg ctggcacagg tatcgcacaa
    15481 ttatctaaag cagaaattaa taatatttac cttcaccgtc aaacatcttt acctgatata
    15541 ttaaaagagt atggatataa aagttatttt atagcttcca ccgaaaaaaa tagcccactc
    15601 aatacaatgt tgaagacact taattttgat aaggttcttg gtatgggtga ttttattggt
    15661 tatcaacgag acagaatgac ggataagcaa acatttaatg cactgcaata ttttttacgt
    15721 tctcaggaaa ataaaaaagg acccttcttt attggtgtct atccatctgg tactcatcat
    15781 ggtcaagata gcccaaatga aaaatatttt gatggtaaca accctctcta caataagttc
    15841 tataactatg attttcagtt agggaaattt attgacttct ttcggcgcag ttcattttat
    15901 aataatacat tattaataat aacgtcagat cattcaacat ttccttcgac agaatataaa
    15961 aaagcattta atagcgattc tcgttacttt gtaggcgaaa taccatttct ggttattgga
    16021 ggagatatta ctcctgaaat tttggatgca gacggtaaaa attcattaag ctttgcgcca
    16081 acaatattgc atatgttagg gattcagtat tcaatgaact attttttggg atgttctctt
    16141 attgataagg aatgcacatc gccaganctg caggcatgca a
//
