LOCUS       EU289392               10355 bp    DNA     linear   BCT 01-DEC-2008
DEFINITION  Escherichia coli serogroup O53 O-antigen gene cluster, complete
            sequence.
ACCESSION   EU289392
VERSION     EU289392.1  GI:167410108
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10355)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O-antigens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10355)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 HongDa Street, Tianjin,
            Tianjin 300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..10355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O53"
     gene            108..1193
                     /gene="rmlB"
     CDS             108..1193
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ABZ79713.1"
                     /db_xref="GI:167410109"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAVMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRLHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYIADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEENYEGRQ"
     misc_feature    108..10189
                     /note="O-antigen gene cluster"
     gene            1193..2092
                     /gene="rmlD"
     CDS             1193..2092
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ABZ79714.1"
                     /db_xref="GI:167410110"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNSE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLDTEKFQQNFA
                     LVLPDWQIGVKRMLNELFTTTAI"
     gene            2150..3028
                     /gene="rmlA"
     CDS             2150..3028
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ABZ79715.1"
                     /db_xref="GI:167410111"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSSRGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            3033..3602
                     /gene="rmlC"
     CDS             3033..3602
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ABZ79716.1"
                     /db_xref="GI:167410112"
                     /translation="MNVIKTEISDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCAVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGLAHGFLVLSETAEFLYKTTNYYHPESDRGIIWDDPDIDVKWPLS
                     IHKPILSIKDEKQKMFKEMIALEKWNGEY"
     gene            3589..4431
                     /gene="wbdE"
     CDS             3589..4431
                     /gene="wbdE"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbdE"
                     /protein_id="ABZ79717.1"
                     /db_xref="GI:167410113"
                     /translation="MENTNYYKLAILIVLYGKEVEQCETFTHIDRVLKKNRMSKDDII
                     LCLWNNGPREIAFNLTTDYYTLKVCQTINNESLSYIYNLFINEVESEKYILLDHDSIL
                     KYDYVLECLNSTSDCIIPNIIVGNNIQSPKTYSIQSFNKGNTVLAIGSGICISKKLCE
                     KLRQVFGDVFDSRFYFYGVDSTFFLRLNKIGESEFIKISECVVLHSLSRLENEAIKVK
                     QFRIKERSYDQGLTLRYYFCKFTFFTVAKKLLAKLIFNKSDISLIYFAKAYFSGKHYK
                     SGKS"
     gene            4428..5540
                     /gene="wbdF"
     CDS             4428..5540
                     /gene="wbdF"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbdF"
                     /protein_id="ABZ79718.1"
                     /db_xref="GI:167410114"
                     /translation="MKFIIIGYRDAYFWNEYGTSVRDLQIAEILSRKYHVTFINRPVS
                     IYERLKNKKKAKSEYKAYSNRITFIDHTSYDLIGPLFGRIWTKYCYKKILNRISSLFT
                     DDSIIIDFTPIAELEFKNNKRITYWYDLIDNFSIHNRFSEKEKKAVRDKYAYVDENAD
                     IITAVSDKAIKNFLNPNKYVMPNGVYLENQNHECFIDGTPNKVYQLGFVGFVTDKLDV
                     DFLNKLSKKFSIIIWGKFFDDSIRDKLNENIKIAGAFKYSDLPSIMKSFKVGLLPYLP
                     EKSHDESPLKMYEYFKYGIPCLSSMNFEIKSRWFVNYLEYSKRDLLYEKINELIVSNN
                     SESIKASIQKEWLFEIKVNKIIEDIIRRKECGKKNS"
     gene            5521..6750
                     /gene="wzx"
     CDS             5521..6750
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ABZ79719.1"
                     /db_xref="GI:167410115"
                     /translation="MVKKIVNQFIYQFGNYILPFLIIPFLINKLSIAVYKDFVVIQSL
                     VLFFAVLINYGLDLFGVRYISNNVKHLRKCKSFLYISILSRLCIFIFCSFFLLVSLMA
                     LTKPDLLYWLLCFFWMLSFVFQSNWYLQGCGDFKLITFYGLLPKLIVYPFVFILVNND
                     NDAWVYLFLCAIANYASNILMMIHIRRKLNVVAFKRTNLLRNVWIFLVKGWYIFLSQA
                     SVTLISYANIFFLPLVLEPKSFVVFSTAERIVKVLSISTTPILNVIFPHISSLIENNK
                     QRVFIMISRISLFSLIVFLLCYTLYVFIGYYLIQLIFPTISQELYSVLLLLLFNVFFV
                     FQNNLFGTQIGLNYGKDKSFTKIIAINGILNIIAMPVFSKLYHLHGTILTSLCIQLSI
                     LICMYMLAKKCGYKFKV"
     gene            6751..7845
                     /gene="wbdG"
     CDS             6751..7845
                     /gene="wbdG"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbdG"
                     /protein_id="ABZ79720.1"
                     /db_xref="GI:167410116"
                     /translation="MKKIAILLPGPRREPVGGYKVLYQYGNYLASVGWQIDFIYLTNR
                     IVCYNRFSGLVGKIKAKLFSLLKFYKWFDFTSSNINHYVIKSLKKRDIENFNIVICSS
                     VETFIYANSSCRVNHKKLVYFVQDYENWNIPENILNYTLSHKDVDYITISDDLVKKIT
                     KMGGDVSLTLYNGINKNDFFVNIPWNERDRASFLFLYHPSERKGCDLLLRAIQKIHEV
                     DSSIKFSCFSAYTKPENFPSFIHYVYQPTVKQLNNLYNENRYFICSSIYEGFGLTAAE
                     ASFSGAIVVTTRNGGVEQFIKNEVTGFVLKERTLNSFMVAIMKLSQKDEELNLFAIES
                     SKKVRIQLDLDINFKKIDSYFKARYNNKRN"
     gene            7849..9141
                     /gene="wzy"
     CDS             7849..9141
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ABZ79721.1"
                     /db_xref="GI:167410117"
                     /translation="MLAWAPKLLLLFFLLSGLIKAISLYININLNYVFIPLLILASLF
                     YMLINRTIKMKAQGACFIFFFSISAILFLVNNIVVNNVYTQLQLMLAVTIQYIFPIII
                     ICLAGSGKLKLDIFDSLINIITKLTFLSLLIFGLCVMYDSKMVEEFYSQLYNHGLIIN
                     PFQTSEEGITLRFSGIFSSGFILASYCCFAIIYYYFNKSVNRIKFAFLFVGFVIMVVL
                     TYNRNGMLAFAVTSLFIMIHTYLRRYYLTLANLYYVFLIGILFIAPSFILYFGGDIQN
                     SYSSIDYSAFTKLSTLFSRIDAWLQVLNINDVSRLLFGSGLVQGLGEDDMNFYVDNGY
                     LYLLHQSGLIALVLYIATWSYMFISLSKNLKKRRNHNFVSVDEINICLLLILVSFTLA
                     LLNNLFFEPVFLILVLMKCVTVNNKSSSIYEKNEQTYE"
     gene            9134..10189
                     /gene="wbdS"
     CDS             9134..10189
                     /gene="wbuV"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuV"
                     /protein_id="ABZ79722.1"
                     /db_xref="GI:167410118"
                     /translation="MSNTAIIGTVGVPASYGGFETLVENLIETEKVNYIVYCSSKNYP
                     KKLSTYKNAKLVYIPLSANGISSVIYDAFSILHAIVFRKVNTLLILGVSGCFILPIIK
                     KISSVKIITNIDGVEWRRDKWGRFAKKFLKWSEKIAVKNSNIVISDNKEIANYVCSEY
                     MVNSQVIAYGGDHSLVFETKRCNDVENSESKYALALCRIEPENNVHVILESFSKVTKK
                     LKFIGNWGSSDYGRSLKKKYSKYNNIEIIDPIYDVEQLNIIRSGCEFYVHGHSAGGTN
                     PSLVEMMFFAKPIICFDCKYNRYTTFEQACYFRDLKDLIIKIESPKERTQGVKLKSLA
                     EKHYTWNRIREQYEQLL"
BASE COUNT     3333 a   1513 c   2015 g   3494 t
ORIGIN
        1 attggtagct gtaagccaag ggcggtagcg tgcattaata cctctattaa tcaaactgag
       61 agccgcttat ttcacagcat gctctgaagt aatatggaat aaattaagtg aaaatacttg
      121 ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata aataatacgc
      181 aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa tcacttgctg
      241 atgtttctga ttctgaacgc tatgtttttg aacatgcgga tatttgcgat gcagctgtaa
      301 tggcacggat ttttgctcag catcagccgg atgcagtgat gcacttggct gctgaaagcc
      361 atgttgaccg ttcaattaca ggccctgcgg catttattga aaccaatatt gttggtactt
      421 atgtcctttt ggaagccgct cgcaattact ggtctgctct tgatagcgac aagaaaaata
      481 gcttccgtct tcatcatatt tctactgacg aagtctatgg tgatttgcct catccagatg
      541 aagtaaataa tacagaagaa ttacccttat ttactgagac gacagcttac gcgccaagca
      601 gtccttattc cgcatccaaa gcatccagcg atcatttagt ccgcgcgtgg aaacgtacct
      661 atggtttacc gaccattgtg actaattgct ctaacaatta tggtccttat catttcccgg
      721 aaaaattgat tccattggtt attctcaatg ctctggaagg taaagcatta cctatttatg
      781 gtaaagggga tcaaattcgc gactggctgt atgttgaaga tcatgcgcgt gcgttatata
      841 ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtgggcac aacgaaaaga
      901 aaaacataga tgtagtgctc actatttgtg atttgctgga tgagattgta ccgaaagaga
      961 aatcttatcg tgagcaaatc acttatattg ccgatcgtcc gggacacgat cgccgttatg
     1021 cgattgatgc tgagaagatt ggtcgcgaat tgggatggaa accacaggaa acgtttgaga
     1081 gcgggattcg gaagacagtg gaatggtacc tgtccaatac aaaatgggtt gataatgtga
     1141 aaagtggtgc ctatcaatcg tggattgaag agaactatga gggccgccag taatgaatat
     1201 cctccttttt ggcaaaacag ggcaggtagg ttgggaacta cagcgtgctc tggcacctct
     1261 gggtaatttg attgctcttg atgttcactc cactgattat tgtggtgatt ttagtaattc
     1321 tgaaggtgta gctgaaactg tcaaaaaaat tcgccctgat gttattgtta atgcggctgc
     1381 tcataccgcg gtagataagg ctgagtcaga acccgaattt gcacaattac tcaatgcgac
     1441 aagtgttgaa gcgattgcaa aagcagccaa tgaggtcggt gcatgggtta ttcactactc
     1501 tactgactac gtatttccgg gaaccggtga aataccatgg caggaggcgg atgcaaccgc
     1561 accgctgaac gtttatggtg aaaccaagtt agccggggaa aaagcattac aagagcattg
     1621 tgcgaagcat cttattttcc ggaccagctg ggtctatgca ggtaaaggaa ataacttcgc
     1681 caaaacgatg ttgcgtctgg caaaagagcg tgaagaatta gctgttatta acgatcagtt
     1741 tggtgcgcca actggcgcag agttactggc tgattgtacg gcacatgcca ttcgtgtggc
     1801 actgaataaa ccggaagtcg caggcttgta ccatctggta gctagtggta ccacaacgtg
     1861 gcacgattat gctgcgctgg tttttgaaga ggcgcgcaaa gcaggcattc cccttgcact
     1921 caacaagctc aacgcagtac caacaacagc ctatcctaca ccagctcgtc gtccgcataa
     1981 ctctcgcctt gatacagaaa aatttcagca gaactttgcg cttgtcttgc ctgactggca
     2041 gattggcgtg aaacgcatgc tcaacgaatt atttacgact acagctattt aatagttttt
     2101 gcatcttgtt cgtgatggtg gagcaagatg aattaaaagg aatgatgaaa tgaaaacgcg
     2161 taaaggtatt attttagcgg gcggttctgg tactcgtctt tatcctgtaa ctatggctgt
     2221 cagtaaacag ctattaccga tttatgataa accgatgatc tattatccgc tctctacact
     2281 gatgttagcg ggtattcgcg atattctgat tatcagtacg ccacaggata ctcctcgttt
     2341 tcaacaactg ctgggtgacg ggagccagtg ggggctaaat cttcagtaca aagtgcaacc
     2401 gactccagat gggcttgcgc aggcgtttat tatcggtgaa gagtttatcg gtggtgatga
     2461 ttgtgctttg gttcttggtg ataatatctt ctacggccac gatctgccga agttaatgga
     2521 cgtagctgtt aacaaagaaa gtggtgcaac agtatttgct tatcacgtaa atgatcctga
     2581 acgctacggt gtcgttgagt ttgataaaaa cggtacggca ataagcctgg aagaaaaacc
     2641 gttacaacca aaaagtaatt atgcggtaac cgggctttat ttctatgaca acgacgttgt
     2701 cgaaatggcg aaaaacctta agccttcttc ccgtggtgaa ctagaaatta ccgatattaa
     2761 ccgtatttat atggaacagg ggcgtttatc tgttgccatg atgggccgtg gttatgcatg
     2821 gctggacacg gggacacatc aaagcctgat tgaggcaagc aacttcattg caacaattga
     2881 agagcgccag ggactaaagg tttcctgccc agaagaaatt gcttaccgta aaggatttat
     2941 tgatgctgag caggtgaaag tattagctga accgctgaaa aaaaatgctt atggtcagta
     3001 tctgctgaaa atgattaaag gttattaatt aaatgaacgt aattaaaaca gaaatttctg
     3061 atgtgctgat ttttgaacca aaagtttttg gcgatgagcg cggcttcttt tttgaaagct
     3121 ttaaccagaa agtatttgaa gaagctgtag gccgcaaagt tgaatttgtc caagataacc
     3181 attcgaagtc tagtaaaggt gttttacgcg ggctgcatta tcagttagaa ccgtatgcgc
     3241 aagggaaatt ggtacgttgc gctgttggtg aggtttttga cgtagcggtt gatattcgta
     3301 aatcgtcacc aacttttggc aaatgggttg gggtgaattt atctgctgag aataagcgcc
     3361 agttgtggat acctgaagga ttagcacatg ggtttttggt gctgagtgag acggcggagt
     3421 ttttatataa aacgaccaat tattatcatc cagagagtga tagagggatt atttgggatg
     3481 atcctgacat tgacgtaaag tggcctttaa gtatccataa accgatttta tctataaaag
     3541 atgaaaaaca aaagatgttt aaagaaatga tagcgttgga gaaatggaat ggagaatact
     3601 aattattata agttagctat cttaattgta ctttatggaa aagaagtaga acagtgtgaa
     3661 acatttactc atatagatag agttctaaaa aaaaatagga tgtcaaagga tgatattatt
     3721 ctgtgtcttt ggaataatgg cccaagagaa atcgcattta atctgactac agattactat
     3781 accttaaaag tttgtcaaac aataaataat gaatcattaa gttatatata taatttattt
     3841 attaatgagg tggagtcaga aaaatatatt ttactagatc atgattccat attgaaatat
     3901 gattatgttt tggaatgtct taattctaca tctgattgta tcattcctaa tataatagta
     3961 ggaaacaata ttcaaagtcc taaaacatat agcattcaat cgtttaacaa gggaaataca
     4021 gtgcttgcta ttgggagtgg aatttgtata tctaaaaaat tatgtgagaa acttcgacaa
     4081 gtatttggtg atgtttttga ttctaggttt tatttttatg gtgttgattc aacttttttt
     4141 ctacggctga ataaaattgg ggaaagtgag tttataaaaa tcagtgagtg cgttgtattg
     4201 cattcattgt ctagactaga aaatgaagct ataaaagtaa agcaatttag aattaaagaa
     4261 agaagttatg atcaaggatt aacattaaga tattatttct gtaaatttac attttttact
     4321 gtcgcaaaaa aacttcttgc gaagttgatt tttaataaaa gtgatataag tttaatatat
     4381 tttgcaaaag catattttag cggtaagcat tataagagcg gtaaatcatg aagtttatta
     4441 ttattggcta tagagatgcg tatttttgga atgaatatgg gacatctgtt agagatttac
     4501 aaattgcaga aattctatct agaaagtatc atgttacatt tataaataga cctgtgtcga
     4561 tatatgaaag attaaaaaat aagaagaaag caaaaagtga gtataaagca tatagtaata
     4621 ggataacttt tattgaccat acaagttatg atttaatcgg accgttattt ggccgcatat
     4681 ggacgaaata ttgttacaag aagatattaa atagaatatc atcgttattt acagatgatt
     4741 caatcatcat tgattttacc cctatagccg aattagaatt caagaacaat aaacgaataa
     4801 catattggta tgatttaata gataattttt caattcataa tagattttct gaaaaagaaa
     4861 aaaaagctgt tcgagataaa tatgcttatg tagacgaaaa tgctgatata attacagctg
     4921 tttccgacaa agcgataaaa aattttctaa atcctaataa gtatgtaatg ccaaatggtg
     4981 tttatcttga aaaccaaaat catgaatgtt ttatagatgg aactccaaat aaggtatatc
     5041 aattaggatt tgttggcttc gttactgata aattagatgt tgatttttta aataaattat
     5101 caaaaaaatt cagtatcatc atttggggaa aattttttga tgattctata cgtgataagc
     5161 tgaatgaaaa tataaaaata gcgggggcat ttaaatactc tgatcttcct tcaataatga
     5221 aatcttttaa agtcggatta ttgccatatc tgccagaaaa atcgcatgat gaatcaccat
     5281 tgaaaatgta tgagtacttt aaatatggca ttccatgttt atcatctatg aattttgaaa
     5341 ttaagagtcg atggtttgtt aattatctag aatactcaaa aagagatttg ttatatgaga
     5401 aaataaatga attaattgtc tcaaataatt cggaaagcat taaagccagc atacaaaaag
     5461 aatggttatt tgaaataaaa gttaataaaa ttatcgaaga tattattcga aggaaagaat
     5521 gtggtaaaaa aaatagttaa tcaatttatt taccagtttg ggaattatat tctacccttt
     5581 cttataattc ctttcttaat caataaactc tctattgcgg tctataaaga ttttgttgtt
     5641 attcaatcat tagtattatt ttttgctgtt ttaattaatt atgggctgga tctgtttggt
     5701 gttagatata ttagtaataa tgtaaagcat ctaagaaaat gtaagagttt tctttatatt
     5761 tccatattgt cacggctctg tatttttata ttttgctcat tctttttgct tgtgtcgttg
     5821 atggcattga ctaaacccga cctcctttat tggttgctat gttttttttg gatgctaagc
     5881 tttgtatttc aatcaaattg gtatttgcaa ggatgtggtg attttaagtt aattacgttc
     5941 tatggcttac ttcctaagct tattgtttat cccttcgttt ttattttggt caataatgat
     6001 aatgatgctt gggtttatct atttttatgt gcaatagcaa attatgcatc aaacatacta
     6061 atgatgatac atattaggag gaagttgaat gtagtagctt ttaaacgtac taatttgctc
     6121 agaaatgtat ggatattttt agttaaaggg tggtatatat tcctatctca agctagtgtg
     6181 acattgattt cttatgccaa tattttcttt ctacctttgg tgttggaacc taaaagtttc
     6241 gttgtctttt caactgctga aagaatcgtg aaggtgttat cgatttcaac aacgcctatt
     6301 ctaaacgtta tttttccaca tatttcatca ttaattgaaa ataataagca aagagtattt
     6361 ataatgattt ctcgaatatc attatttagt ttaattgttt ttctgttatg ttacacgttg
     6421 tatgttttta taggctatta tctcatacag cttatttttc caacaatatc acaagaatta
     6481 tactctgtat tattattgtt gttatttaat gtattcttcg tattccaaaa caatctcttt
     6541 ggtacacaaa ttggattgaa ttatgggaaa gacaaaagct tcactaaaat tattgcaatc
     6601 aatggtattt taaatattat agccatgcct gtatttagta aactgtatca cctgcatggc
     6661 acaatattaa catccttatg catacaatta tcaattttga tatgtatgta tatgttggct
     6721 aaaaaatgtg gttataaatt taaggtctaa atgaaaaaaa tagctatttt actaccagga
     6781 ccaagaaggg agcctgttgg tggatataaa gtcttgtatc aatatggaaa ctatttggca
     6841 tctgttggat ggcagattga ttttatttat ttgacgaacc gtattgtttg ttataatcga
     6901 ttttctggcc ttgtgggtaa aataaaagca aaattatttt cattattaaa gttctacaaa
     6961 tggtttgatt ttacatcctc taatataaat cattatgtga ttaaatctct aaaaaaaaga
     7021 gatatcgaaa attttaatat tgtcatttgc tcctctgttg agacgtttat atacgccaat
     7081 tcgtcatgta gagttaacca taaaaaatta gtatattttg tacaggatta tgaaaactgg
     7141 aatattcctg agaatatttt aaactataca ctttcacata aagatgttga ttatataact
     7201 atttctgatg acttggtgaa aaagattaca aaaatgggag gggatgtttc tctcaccttg
     7261 tacaatggaa taaacaaaaa tgattttttt gttaacattc catggaatga aagggataga
     7321 gcttcatttt tatttctata tcatcctagt gaaagaaagg gttgtgatct cttattaaga
     7381 gcaatacaaa aaattcacga agttgatagc tcaattaaat tcagttgctt tagtgcttat
     7441 acaaaaccag aaaatttccc ttcgtttatt cattacgtat atcaacccac agttaaacag
     7501 ctaaataatt tatataatga gaatagatac ttcatttgca gtagtatata tgaaggattt
     7561 ggtttgactg cagcagaggc atcttttagt ggggcaatag tagttacaac gagaaatggt
     7621 ggcgtagaac aatttatcaa aaatgaagtg actggctttg ttctcaagga aagaacgcta
     7681 aattccttca tggttgctat tatgaagctt tcacaaaagg atgaagaact aaatcttttt
     7741 gctattgaaa gtagtaaaaa agtcagaata caactagatc tcgatataaa tttcaagaaa
     7801 attgatagtt actttaaggc tagatataat aacaagagga attaatttat gctagcttgg
     7861 gcaccaaagt tgttattgct gtttttttta ttgtctggat taattaaggc tatatcattg
     7921 tatataaata ttaatcttaa ttatgtgttt attcctctat taatattagc ctctctgttt
     7981 tatatgttaa ttaacagaac aataaaaatg aaagctcaag gggcatgttt tatatttttc
     8041 ttttccatat ctgcaatttt gtttcttgta aataacatcg ttgttaataa tgtttataca
     8101 caattacaat taatgcttgc agtaacgata caatatattt ttccaattat aatcatttgt
     8161 ttagccggtt cgggaaagtt gaaattggat atttttgact ctttgattaa tataataaca
     8221 aagcttacct ttttatcact tttaatattt gggttatgtg tgatgtatga ctctaaaatg
     8281 gttgaagagt tttattcgca attatacaat catggtttaa taattaatcc atttcaaaca
     8341 agtgaagaag gtattactct tcgttttagt gggatatttt catctgggtt tatcttagct
     8401 agttattgtt gctttgcaat aatttattat tattttaata aatctgtcaa tcgtataaag
     8461 tttgcttttc tatttgttgg ttttgtcata atggttgttt tgacatataa tagaaacggg
     8521 atgttggcat ttgcagttac atctttattt ataatgattc atacttacct tcgtcgatac
     8581 tatctaactc ttgcgaattt atattatgtt tttttgattg gaatattgtt catagcacca
     8641 agttttatat tatactttgg tggtgacatt cagaattcat attcttctat agattattca
     8701 gcttttacaa aattgagtac attattctct cgtatagatg cctggcttca ggttttgaat
     8761 attaatgacg tctctcgttt attatttggt tccggtttag tgcaggggtt aggggaagat
     8821 gatatgaatt tttacgtcga taatggttat ttatatcttt tgcatcagag tggtcttata
     8881 gctcttgttt tatatatcgc tacttggagt tacatgttta tttctctctc taaaaactta
     8941 aagaaaagac gaaaccataa ttttgtttca gtagatgaaa taaatatatg cttacttctt
     9001 attcttgtaa gtttcacctt ggcactcctt aataatctat tttttgaacc cgtatttcta
     9061 attcttgtat taatgaaatg tgtaactgtg aataataaat caagtagtat ttatgaaaaa
     9121 aatgaacaaa catatgagta ataccgccat tattggtact gtaggtgttc ctgcttcgta
     9181 tggagggttt gaaacacttg ttgaaaattt aattgaaact gaaaaggtta attatattgt
     9241 ttattgctca tcaaaaaatt accccaaaaa actaagcaca tataaaaatg cgaaattggt
     9301 ttatattcct cttagcgcta atggaatatc aagtgttatt tatgacgcat ttagcatatt
     9361 gcatgcgatt gtattccgaa aagtaaatac actccttatt ttaggagtct ctggttgctt
     9421 tattcttcct ataataaaaa aaatatcatc tgttaaaata attacaaaca ttgatggagt
     9481 agagtggcgt cgagataaat ggggtaggtt tgctaaaaaa tttttaaaat ggtctgaaaa
     9541 aatagctgtg aaaaattcta atattgtgat ttctgataat aaggaaatcg caaactatgt
     9601 gtgctcagaa tatatggtaa attctcaagt aattgcctac ggcggagatc attcactagt
     9661 ttttgaaaca aaaagatgta acgatgtgga gaattccgaa agtaaatatg ctcttgctct
     9721 ctgtcgtatt gagccagaaa acaatgttca tgttatatta gaatcttttt ctaaagtcac
     9781 caaaaaattg aagtttattg gtaattgggg aagtagcgac tatgggcgta gtcttaagaa
     9841 aaaatattca aagtacaata atatagaaat tattgatcca atttatgatg ttgaacaatt
     9901 aaatattatt agaagcggat gtgagttcta tgtgcatgga cattctgctg gaggtactaa
     9961 tccatcacta gttgaaatga tgttttttgc aaagccgatt atttgttttg attgtaagta
    10021 taatcgttat acaacatttg aacaagcgtg ttatttcaga gacctgaagg atcttataat
    10081 taaaatagaa tcacccaaag agagaacaca aggtgttaag ttgaagtcgc tggctgaaaa
    10141 acattacaca tggaatagaa ttcgtgaaca gtatgagcag cttttgtaaa aatggagtaa
    10201 gaatattaag aacatataga tatttcaggt aggtaaagtt gaggttataa ggtactcttc
    10261 aaacttgatt caagccgcgc aaacccgtgg tgaacacccc ctgacaggag taaacaatgt
    10321 caaagcaaca gatcggcgtc gtcggtatgg cagtg
//
