LOCUS       DQ220293               13075 bp    DNA     linear   BCT 30-NOV-2006
DEFINITION  Escherichia coli O56 O antigen gene cluster, complete sequence.
ACCESSION   DQ220293
VERSION     DQ220293.1  GI:78191383
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13075)
  AUTHORS   Cheng,J., Wang,Q., Wang,W., Wang,Y., Wang,L. and Feng,L.
  TITLE     Characterization of E. coli O24 and O56 O antigen gene clusters
            reveals a complex evolutionary history of the O24 gene cluster
  JOURNAL   Curr. Microbiol. 53 (6), 470-476 (2006)
   PUBMED   17072668
REFERENCE   2  (bases 1 to 13075)
  AUTHORS   Cheng,J., Wang,Q., Wang,W., Wang,Y., Wang,L. and Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2005) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hong Da Street, TEDA, Tianjin
            300457, P.R.China
FEATURES             Location/Qualifiers
     source          1..13075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O56"
     gene            2511..3131
                     /gene="nnaD"
     CDS             2511..3131
                     /gene="nnaD"
                     /note="N-acetylneuraminic acid synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="NnaD"
                     /protein_id="ABB29909.1"
                     /db_xref="GI:78191384"
                     /translation="MKKRLIIIGAGGFAKAVIDSLDHEQYEVYGFIDSFKSGEHQGYK
                     ILSKTIDGLNEPNQYCYFIAIGEPCCRAIWLETINNMRLETINVIDKTAIVSKRAILG
                     TCIYVGKMAIVNCDSHLEDGVVINTRALVEHGNYISYCSNISTNVVLNGDVFVGRKCF
                     IGSCTVVNGQLKIGDSSIVGSGSVVIRNIDKNIVVAGTPTRLIRKR"
     misc_feature    2511..11752
                     /note="O antigen gene cluster"
     gene            3136..4173
                     /gene="nnaB"
     CDS             3136..4173
                     /gene="nnaB"
                     /note="N-acetylneuraminic acid synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="NnaB"
                     /protein_id="ABB29910.1"
                     /db_xref="GI:78191385"
                     /translation="MSSVYIVAEIGCNHNGDFNLAKRMIEEAYNAGVDAVKFQTFKAD
                     SLISKYAPKADYQIKTTGNSESQLEMTRKLELSYKEYIKLEEYCKSLGLDVFSTPFDF
                     ESIEFLYTRKQTTWKIPSGEILNLPYLEKIALLPINKKIILSTGMATIDEIKQALKVF
                     TDNGINSEDITILHCNTEYPTPYEDVNLNALAGLKKTFQGYNIGFSDHSPGFFAGIAA
                     VPYGITFIEKHFTLNKNFEGPDHKASVTPDELRLLCEGIRAIEKSLGSFDKLVTDSER
                     KNKIVARKSIVAKRDIKKGEVFTVDNITTKRPGNGISPMYWYEVLGKKAEQDFLEDQL
                     IKMSGVDEQEI"
     gene            4176..5438
                     /gene="nnaC"
     CDS             4176..5438
                     /gene="nnaC"
                     /note="CMP-N-acetylneuraminic acid synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="NnaC"
                     /protein_id="ABB29911.1"
                     /db_xref="GI:78191386"
                     /translation="MRKNKIAIIPARSGSKGLLNKNILMLCDKPLIAYTIEAAIESKV
                     FDKVIVSTDSTEYKDIALRYGAEVIMRDKELASDKATSFVVVKDVLEKNNGYDYFALL
                     QPTSPFRNYKHIREAINLFENTVEIDFLVSMVKTNKSAELINQLTPNNTLKYFNADFS
                     NYRRQNQDNYCPNGAIFIGNVDAYIKKKSFFGERSIAYIMDRIDSIDIDDKLDFEFAI
                     TIMHKKNRSRELQKKILNRICEKKNYFNQKKDITLMGHSIFDYWDITQLKKRDVNNLG
                     IAGINSKQYYDYIIKKNIAKVGQDVILMLGTNDIVTNDWNLDFTTNWIEKIINRLKRI
                     NPNVNIYILSVPPVVGRIDRNNGVIKKLNCNLLKYFDNCQNVFFLPLSEAFYDKYGNL
                     KEEFTYDGLHFTQLAYNQLKKEIEEKLK"
     gene            5435..6625
                     /gene="nnaA"
     CDS             5435..6625
                     /gene="nnaA"
                     /note="UDP-N-acetylglucosamine-2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="NnaA"
                     /protein_id="ABB29912.1"
                     /db_xref="GI:78191387"
                     /translation="MKKLLYITGSRAEYGIMKRLLRKLQDHERIELSIIATGMHCDVK
                     YGYTYQNIIDDGFEIKELFDIKIETSTNSNIISTMSRAQSLFGAHFQDNKYDAIIILG
                     DRYEMLSVAIAASIHGIPIIHLHGGEQTLGNYDEFIRHCITKMSHLHLVATDKYRERV
                     IQLGEPPASVVNIGAMGAENVIYLPIPEHNELQSKYSNVLSNYFMVLFHPETLTRKSV
                     HDQINELIKALEIAHKKNHCNFIFIGSNSDTSSEIITEQILTFCKSYNFPYLVSLPTQ
                     DYLGLCKYSLGLIGNSSSGLIEIPSLGVPTINIGHRQAGRERGKSVIDVECCHVDILN
                     KIHDILSNRNISADDNVNPYYKENSADLAMSSIISFLDRIGDNKSHIKKFYDIPNIDN
                     LINS"
     gene            6643..7398
                     /gene="wfaP"
     CDS             6643..7398
                     /gene="wfaP"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfaP"
                     /protein_id="ABB29913.1"
                     /db_xref="GI:78191388"
                     /translation="MELVSIIIAAYNCKDTIYATVESALSQTYKNIEIIICDDSSTDD
                     TWDIINKIKDSRIICIKNNYCKGAAGARNCALKIAKGRYIAFLDSDDYWVTTKISNQI
                     HFMETEKVFFSYSNYYIEKDFVITGVFSSPPEINYGAMLKYCNIACSTVILDRTGVKN
                     ISFPYIDKEDYALWLNILSKGIKARNTNLVDTYYRVHAGSVSANKFKELIRQSNVLKS
                     IGIKAHHRIICLFYYAINGLIKHCFSYRDKRNA"
     gene            7391..8479
                     /gene="wfaQ"
     CDS             7391..8479
                     /gene="wfaQ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfaQ"
                     /protein_id="ABB29914.1"
                     /db_xref="GI:78191389"
                     /translation="MLRVCFIIADVTWTGGIERVLSRLALSIPDDNIDLEILSLYRTY
                     KDTYYTFPDNVKIKYLNDNYQYKGRPGSFTRLVQHLRTSIKLREFLNSCNYDIIVVHT
                     FPLAFINFFTEKRSKWVVVEHVKYYYYNILLRRIRSFIYDKYDAIVLLTDKDVTHFKN
                     ANNNVYVIPNPLSFSQTKKANLNSVRIISVGRLEKQKGFDMLLKAFSYISYKYPEWQL
                     DIFGKGNEEENLRKLISKLNLKNVNLMRTSKNIHQEYLSSAFYVMSSRYEGFPMVLLE
                     AMASGLPCISFNCETGPADIIIDNENGFLIEHFDVVQLSKAMEKLIKDEYLRTKMGNA
                     AFERADEFSLEKITLKWKELFKGLTHEK"
     gene            8469..9476
                     /gene="wfaR"
     CDS             8469..9476
                     /gene="wfaR"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfaR"
                     /protein_id="ABB29915.1"
                     /db_xref="GI:78191390"
                     /translation="MKSNVYICKTTWEIFVSILLASDVYYSKNIKSVFVIEVAEENIN
                     YIPRLSDFCFILKVVSVKFSSNKYIKALCFLYRVKYFLPKVLGEYLKNDVRVNLFIDQ
                     GVFGQFFLKNHKDVYLYEHGNGNYCVGPYPNYKLIKKILGITEGYGRHSRVKRVYLQH
                     PEKAPQDIRNKVIGFSIKHLYCKLCSREQQALLSFFGVKDINKSNDIIILTQPISEDG
                     FYSEKEKIEIYKEIINSRFSHDKSRVVIKPHPRDKTDYSKYIPDVMLLPRNFPMEILN
                     FTDVRFVKAITICSSAVYNFGYDISVDYIGTLKYPKLIKALGRLPAQYVHKDNYKKIK
                     Y"
     gene            9489..10538
                     /gene="wzy"
     CDS             9489..10538
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ABB29916.1"
                     /db_xref="GI:78191391"
                     /translation="MIPYIIFYSLSLTLVVLSLVLKKYDWFWLLFLLFISIIFIGLRV
                     NVGADYTEYVRIYNQSYDTNFELGFDIIFNLFKRFGYDYVFVSLFVFILTSFFFLYSI
                     KSLQHKTLIYFCFLLFMFVPLTSTIRQGLAIPFFIICLLNADRPKVYFMSIAIGCFFH
                     YSILFMLFFFFVRYFKQSYCRAYFIILFFLGLSVFNIVEYMIILLHYIPSLGTLSSKL
                     LMYTQRYNVTLSLTSVAYKLAGLLLVTSFMSFIKINKKLLCSYNLYYISLCLFILFKD
                     NSVFTNRLMYATNISLVTFFSFLLSMMNAFYRKAIVTFLFVIYFSINYFKFTATDLRH
                     SIESAYMPYKSVLIN"
     gene            10553..11752
                     /gene="wzx"
     CDS             10553..11752
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ABB29917.1"
                     /db_xref="GI:78191392"
                     /translation="MKLMKDRFFYFFIELINKGVPFFLLPYVSSAVGVEVYGKIELFT
                     TNYIILSFLFALGFEGWMNSHYFKISENNFITILSSFYFLSFLIFFISVITTLLYSSD
                     WTALVIVGYIQSLLNITTLHLRLKGKYIRSGALLLAASIVNAVMVYVCFELFGKDYNA
                     RIEAFLFSSVLLVIFIILCNINIWGGGGKNKINFSFLNKDILLFCLPLCLTMVINWAK
                     GNIDKYFIANLLDLKSLGIYAVAYQLASVINVVGIILNRTLQADLLKSMADGFYQTKK
                     IILIFLSVLAIFFLLYVIAVCYGFNYVFSADYAPSRNMALLLIFCFLFFSTSSFLINF
                     VLFYGRPKLILLQSLLITTIHVALSFLLIQKYALFGALFTQVITSILTFISTLLLCYI
                     LMKASHK"
BASE COUNT     4305 a   1908 c   2486 g   4376 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ttggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaactgcg cgtgaagcgt
      121 caactgctgg cggaagtaca gtccatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt aggccactcc attttatgtg cacgacctgc cattggtgac
      241 aatccgtttg tcgtggtact gccggacgtt gtgatcgacg acgccagcgc cgatccgctg
      301 cgctacaacc ttgcagccat gattgcgcgc ttcaacgaaa cgggccgcag tcaggtgctg
      361 gcaaaacgta tgccgggcga tctctctgaa tactccgtta ttcagaccaa agaaccgctg
      421 gatcgtgaag gtaaagtcag tcgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagatatcat ggcagtaggt cgttatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcagcc tggtgcatgg gggcgtattc agctgactga tgccatcgct
      601 gaactggcga aaaaacagtc cgttgatgcc attctgatga caggtgacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttt ttgaagtatg ggctacgcaa ccacaaagaa
      721 ggggcgaagt tccgtaaata tattgaagag ttattgagta aaagttattg actggataat
      781 tgaaaatatt aaataattgt ttaactttta taatctacta attatgaaaa ctggttaaag
      841 tatttaggga tctagtaact attaagctag gggcggtagc gttgtctacg gttcgtatta
      901 tttacatatt taaagcactc cagagagatg ttaactcctc ctgtaagaca attatctgaa
      961 gaaagtcatg tctaatatat gaattcactc agatgagtac aaagatgaga aacagatacg
     1021 agaagggaaa gaacatcaga gatacctttt tatactaaat aaatcacatt gaatattcaa
     1081 taattgagat attccttatt aggtaatgat tgttttttaa tgagctgtat atgacacaaa
     1141 tattatttgt ataaataaag aagagtaaaa agaaaattaa aaattgataa aatatatttc
     1201 tattatcccc ggttacagac cgtcttataa agtagaacat ctacttttga ctatttgcgc
     1261 tattattcat ggtgcagatg gttggggtat ataaatgttt tttgggcaac gcttctagat
     1321 ttttgaagta atatggtgat cttaaaagtg gtattcctgt tcataatacc attgtcagag
     1381 tggtatcatg cttcattcct gcaaataatt actggtgctt tattaactaa gtaagtgact
     1441 attattcttc aaatgatgaa gatgtcgttg caattgatgg agaatgtact ggctctttta
     1501 tgataagagt cgcagcaagg gtacgattta tgttattaat gtgttttaac aatgcacaat
     1561 ctggtcctag gataacggat gagtaatcca atcaaagcta ttcctaatat tcttaacata
     1621 ttagatatta gaggataatt atcatgattg gcgcggcggt gggcgagaaa tatcttgtgg
     1681 tgaagataca aaagcaagga agtgattatt tattcgctgt aataatcaga ggctgcttaa
     1741 taaagtattt gaccactgaa agaattgaat aattcaaagc aatgactgtt acgtaatcag
     1801 tgaaaggcgt catgacaaaa aagaaaaccg tcttcatatt atttgcgata cccctgataa
     1861 aattatttat ttcactgttg aatgaaatga atgatgcgtg acagctatct ttagctaaat
     1921 aatagtagag caaatgaaag aactagaaat gatggtcaga tattatatca gttctgatga
     1981 taaaacagcg tagaggttcg ctacaattat ccgaaatcac tggcgagtgg agaataagtg
     2041 tactggaagc agaaagtggt gatgaatgaa tacgactgcg aaattacctc gcgtgagagc
     2101 ttgcgggggg gaggctaaat ttggtcctgt gtgcatcttg tattgagata aacatcaaaa
     2161 atttagattt caatataata atgatgtacc tgtaagtcac agcataatca aaccttgtga
     2221 tcgatttttc atcttagaag gaatttatgg aaaatttttt tgtatgtgtg ttaccaattg
     2281 ttggtacata agcgtagact aagtaattat tagattagat ataaaacgct aagaaaacat
     2341 agaacaaacc tatgaacaaa aatggagttg ctgcgtttaa tataaatatg gaatataacc
     2401 ctcgcctctt tctaggaggt attctgataa acaaccattc taggaaatga actttattat
     2461 ttttggttga tttttggact ctggataata agacatcgaa tggcatatat atgaagaaaa
     2521 gattaatcat cattggagct ggcggttttg caaaagcagt cattgatagc ttagaccatg
     2581 aacagtatga agtttatggt ttcatcgaca gctttaagag tggggaacat caaggctata
     2641 aaatactttc aaaaacaata gatggtttaa atgaacctaa tcaatactgt tattttattg
     2701 cgattggcga accctgttgt agggctatat ggctagaaac tattaataat atgaggcttg
     2761 aaacgatcaa tgtcattgat aaaacagcaa tagtctcaaa acgagcaatt cttgggacat
     2821 gtatctatgt cggtaagatg gcaatcgtta attgtgattc acatcttgag gatggcgttg
     2881 ttattaatac aagagctctt gttgagcatg gtaattatat atcatattgc tccaatatat
     2941 ccaccaacgt cgtattgaat ggagatgttt ttgttggtag aaaatgtttt ataggtagtt
     3001 gtactgtagt taatggtcaa ctgaaaatag gcgattcatc tatagttggc tctggctcag
     3061 ttgttattag aaatatagat aaaaatattg tcgtcgctgg tacaccaaca agacttatta
     3121 ggaaaaggta ataaaatgtc atctgtatat atagtcgcgg aaattggatg taatcataat
     3181 ggagacttta atttagctaa aaggatgata gaggaggctt ataatgctgg ggtcgatgct
     3241 gttaaatttc agacatttaa agccgatagt ttaatatcaa aatatgctcc taaagcagat
     3301 tatcagatta aaacgacagg taatagtgag tcacagttgg aaatgactcg gaaactagag
     3361 ctttcctata aagaatatat caagttagaa gaatattgta aaagtttagg gttggatgtt
     3421 ttttcaaccc cctttgattt tgagtcaata gaatttttat atacgagaaa acaaaccaca
     3481 tggaagatcc catctggtga gatactgaac ctaccatatt tggagaaaat tgcattgttg
     3541 cctattaata agaaaattat tttatctaca ggcatggcaa ctattgatga aataaagcag
     3601 gctctgaaag tatttactga caatgggatt aattctgaag atatcacgat attacattgt
     3661 aacactgagt atcctacgcc ttatgaagac gttaatctca atgctctcgc gggtttaaaa
     3721 aaaacattcc aaggatataa cattggtttt tcggatcatt ctccagggtt ctttgctggt
     3781 atagcagctg ttccttatgg aataactttc attgaaaagc attttacatt aaataaaaac
     3841 ttcgaaggac cagatcataa agcatctgtt actcctgatg aattaagatt gttatgtgaa
     3901 ggtattagag cgattgaaaa atcattggga agttttgata agttagttac tgattcggag
     3961 agaaaaaata aaatagtggc gaggaagtcc attgtagcca aacgtgatat caaaaaaggg
     4021 gaagttttta ctgtcgataa tattactact aaacgcccag gaaacggaat cagtcctatg
     4081 tattggtatg aggttttggg taaaaaagct gagcaagatt tcttagaaga tcagctaata
     4141 aaaatgtctg gtgttgacga acaagagatt taaaaatgag aaaaaataaa attgcaatta
     4201 ttcctgctcg ttcaggatca aaagggctac tcaataaaaa tatattaatg ctttgtgata
     4261 aacccttaat tgcttataca atagaagctg ctattgaaag taaagtattt gacaaagtta
     4321 ttgtttcgac ggattctact gaatataagg atattgcttt acgttatggg gctgaagtta
     4381 ttatgaggga taaagagctg gcctctgata aagctacatc atttgtggtt gtgaaagatg
     4441 tgttagaaaa aaataatggc tatgattatt ttgctttatt acagccaaca tcaccattta
     4501 gaaactataa acatattagg gaagctatta atctttttga aaatacagta gaaattgatt
     4561 ttttggtctc aatggtcaaa acgaataaaa gtgcagagtt gattaatcaa ttgacaccca
     4621 acaatacttt gaaatatttt aatgcggact tttctaatta tagaaggcaa aaccaagata
     4681 attactgtcc aaatggtgcg atatttatag gcaatgtgga cgcatatatt aaaaaaaaga
     4741 gtttctttgg tgaaagaagt attgcctata taatggatag aatcgattca atagatattg
     4801 atgacaaact agatttcgag ttcgcaataa caataatgca taaaaaaaat agatctagag
     4861 aacttcaaaa gaaaatacta aataggatat gcgaaaaaaa aaattacttt aaccaaaaaa
     4921 aagatataac gttaatgggg cattcaattt tcgactattg ggatataact cagttaaaaa
     4981 aacgtgatgt gaataattta ggaatcgctg gtattaatag taaacaatat tatgattata
     5041 ttataaaaaa aaatatagca aaggttggtc aagatgtcat attaatgctt ggtacgaatg
     5101 atattgttac taatgattgg aatttagact tcacaactaa ttggattgaa aaaataatta
     5161 atcgcttaaa aagaataaac ccaaatgtta atatttatat tttatccgta ccccctgtag
     5221 ttggacgaat tgataggaat aacggagtaa taaaaaaatt gaattgcaat ctgttaaaat
     5281 attttgataa ttgtcaaaat gtgttttttt tacccctgtc agaagctttt tatgataaat
     5341 atggaaatct aaaggaagag ttcacatatg atggattgca ttttactcag ctagcttata
     5401 atcaactgaa gaaagagatc gaggaaaaat taaaatgaaa aaattattat atataactgg
     5461 ttcaagagcg gagtatggga tcatgaaacg gttgttaaga aaacttcagg atcatgagcg
     5521 tattgaactt tctattattg caacaggaat gcattgtgat gttaagtatg gttatacata
     5581 tcaaaatatt attgacgatg gatttgaaat aaaagaatta tttgatataa aaattgaaac
     5641 atctacaaat agtaatatta tttctacgat gagtcgtgca caaagtttgt ttggtgctca
     5701 ttttcaggac aacaaatatg atgcaataat tattcttggt gatagatatg aaatgctttc
     5761 tgtagctata gctgcatcta tacatggaat tccaattatc catttacatg gaggagaaca
     5821 aacattaggg aattatgatg aattcattcg tcactgtatt acaaaaatga gtcacctaca
     5881 tttagttgca acagataaat atagagaaag agtaatacaa ctaggagaac cacctgcttc
     5941 tgttgtaaat atcggagcga tgggggctga gaatgtaata tatttaccta taccagaaca
     6001 taacgaacta cagtcaaaat atagtaatgt tttaagtaat tattttatgg ttctttttca
     6061 tcccgagaca ttaactagaa aatctgtgca tgaccaaata aatgaactaa ttaaagcatt
     6121 ggaaattgcg cataaaaaaa atcattgtaa ctttatcttc ataggatcta actcggacac
     6181 gagttcagaa atcattactg agcaaatttt aaccttctgt aagtcataca atttcccata
     6241 tttagtatcg ttaccgactc aagattattt aggattatgt aaatattctt tagggcttat
     6301 tggtaattca tcttctggcc taatagaaat tccatcactc ggagtcccta ctattaatat
     6361 tggacatcga caggctgggc gagaacgtgg aaaatcagtg attgatgttg aatgctgcca
     6421 tgttgatata cttaataaga tacatgatat tttatcgaat cgaaatatca gtgctgatga
     6481 taatgttaat ccttattata aggagaacag tgctgactta gctatgtcta gtataattag
     6541 ttttctagac aggattgggg acaataaaag tcatatcaaa aagttttatg atattcctaa
     6601 tattgataat ctgattaact cataataagt aaacatggtt atatggaact agttagcatc
     6661 attatagcag cttataactg taaagatacc atctatgcta cagttgaatc agcgctatct
     6721 cagacataca aaaacattga aataataatt tgcgatgact catctacaga tgatacatgg
     6781 gatataatta ataaaattaa agactcacga attatttgca taaaaaacaa ttattgtaag
     6841 ggggcggcgg gagcaagaaa ttgtgcatta aaaatagcca aaggcagata catagcattt
     6901 ctggatagtg atgattactg ggttacaact aagatatcaa atcagatcca tttcatggaa
     6961 acagaaaagg ttttcttttc atacagcaat tattatattg aaaaagattt tgttattacg
     7021 ggcgtcttta gttctccgcc tgaaatcaat tatggtgcca tgcttaaata ttgtaatatt
     7081 gcatgttcaa cagtcatcct cgataggacg ggggtaaaaa atatatcttt cccttacata
     7141 gataaagaag attatgcatt atggcttaac atcctttcaa aaggaataaa ggctaggaac
     7201 actaatttag tagatacata ttatagagtt catgctggtt cagtttcggc aaataagttt
     7261 aaagagttaa tacgacaaag taatgtttta aagtctatcg ggatcaaagc acatcacaga
     7321 ataatatgtt tattttatta tgctataaat ggtttgatta aacactgttt tagttataga
     7381 gataaaagaa atgcttagag tttgttttat tattgcagat gttacatgga caggcggaat
     7441 agaacgagtg ctatctaggt tagcgttatc gattccagat gataatatag acttagaaat
     7501 attaagctta tataggactt ataaagatac atattataca tttccagata atgttaaaat
     7561 caaatatctt aatgataact atcaatataa aggtcgacca ggatcgttca ctcggttagt
     7621 tcagcatcta agaacatcta ttaaactaag ggaatttcta aattcgtgta actatgatat
     7681 tattgtggtt catacatttc cgcttgcatt catcaatttt tttactgaaa aaagatcaaa
     7741 gtgggtcgtt gttgaacatg taaaatatta ttattataat attttattaa gacgtatacg
     7801 ctcatttata tatgataaat atgatgctat tgtgttatta acagataaag atgttaccca
     7861 tttcaaaaat gcgaataata atgtatatgt aatacctaat cctttaagtt tttcgcaaac
     7921 taaaaaagcg aatctgaata gcgttagaat aatatctgtt ggaagattag aaaaacagaa
     7981 gggattcgat atgctcttaa aggcattctc atatatatca tataaatatc cagaatggca
     8041 attggatatt tttgggaaag gtaatgaaga agaaaatctg aggaaactta tatctaaatt
     8101 aaatttaaaa aatgtgaatc ttatgagaac gtcaaaaaat attcatcaag aatatcttag
     8161 ttctgcattt tatgtaatgt cttcacggta tgaaggtttt cctatggtac tcttggaggc
     8221 gatggctagc ggtttaccat gtatatcatt taattgtgag actggccctg cagatattat
     8281 tattgacaat gaaaatggtt ttcttataga acactttgat gttgttcaat tatcaaaagc
     8341 aatggagaaa ctgatcaaag atgaatattt gagaacaaaa atgggtaatg ccgcatttga
     8401 gcgagcagat gaattttcat tagaaaaaat cacattgaaa tggaaggaat tatttaaagg
     8461 gcttactcat gaaaagtaat gtttatattt gtaaaaccac atgggaaatt tttgtttcta
     8521 ttctattggc tagcgatgtt tactatagta aaaatataaa atcagttttt gttattgaag
     8581 ttgctgagga aaatattaat tatattccta ggctgtctga tttttgcttt atattaaagg
     8641 ttgtttctgt taaattttct tctaataaat acattaaagc tctgtgtttt ttatatagag
     8701 ttaaatattt tttacctaaa gtacttggag agtatctaaa aaatgatgta agggttaatc
     8761 tgtttataga tcaaggtgta tttggacagt ttttcctgaa aaatcacaaa gacgtttatc
     8821 tatatgagca tgggaatgga aattattgtg tgggaccata tcctaattat aaactaataa
     8881 aaaagatttt aggtataact gaaggttatg gtagacacag tagagtgaag agggtatacc
     8941 tacagcatcc ggaaaaagct cctcaagata ttcgtaataa ggtaattggt ttttctatta
     9001 aacatttata ttgtaaatta tgctcgagag agcaacaagc cttattgagt ttttttggag
     9061 tgaaagatat taataaatca aatgatatta ttattttaac acagccaatc agtgaggatg
     9121 gtttttacag tgaaaaagag aagatagaaa tttacaagga gattattaat agccgctttt
     9181 cccatgataa atctagagtt gttataaagc cgcatccccg cgataaaaca gattatagta
     9241 aatacatacc agatgtaatg ctattgccaa gaaattttcc tatggaaata ttaaatttta
     9301 cagacgtgag atttgttaaa gctataacaa tttgttccag tgctgtatat aattttggat
     9361 atgatatttc agttgattat ataggtacct taaaatatcc aaaattaatt aaagcgttgg
     9421 ggcgattacc tgcacaatat gtacataaag ataactataa aaaaatcaag tattaattgg
     9481 agtctgatgt gataccttat attatatttt attctttatc attaactttg gttgtattat
     9541 ctttggtgtt aaagaaatat gattggtttt ggttgctttt tcttttgttt atttcaatta
     9601 tttttattgg ccttagggtg aatgtaggcg ctgattatac tgaatatgtc agaatttaca
     9661 atcaatctta cgatactaat tttgaattag ggtttgatat aatatttaat ctctttaaga
     9721 ggttcggtta tgattatgta tttgtatctt tgtttgtgtt tatattgaca tcattttttt
     9781 ttctatattc aattaagtca ttacaacaca aaactttaat ttatttctgt tttttgcttt
     9841 ttatgtttgt ccctctaaca tcaactatta gacaagggtt agctattcct ttttttataa
     9901 tttgcctttt aaacgctgat agacctaaag tctactttat gagtatcgct atagggtgtt
     9961 ttttccatta ttcgatttta tttatgctat ttttcttttt tgttagatac tttaaacaat
    10021 cgtattgcag agcttatttt ataatcctgt tttttttagg tttaagtgtt tttaatatcg
    10081 ttgagtatat gattatacta ttacattata ttcctagtct gggaacgtta agctctaaac
    10141 tccttatgta tacacagcga tataatgtta cgctttcatt aacttcagtt gcttataaat
    10201 tagctggttt actattggtt acgagtttta tgtcttttat caaaataaat aaaaagttac
    10261 tgtgtagtta taacttatat tatatatcat tgtgtttgtt tatcttattc aaagataatt
    10321 ctgtttttac aaatcgttta atgtatgcca ctaatatatc cttggtgact tttttttcat
    10381 ttttgttatc gatgatgaat gcgttttata ggaaagccat tgttactttt ttgtttgtaa
    10441 tctatttttc cataaattat tttaaattta cagcaacaga tttaagacac tctattgagt
    10501 ctgcttatat gccttataaa tcggtactta taaattaagt aaaatataaa tcatgaaatt
    10561 aatgaaagat agattttttt atttttttat cgaactaata aataaaggcg tcccgttttt
    10621 cttattacct tatgtttctt ctgctgtcgg tgttgaagtc tatggtaaaa tcgagctttt
    10681 tactactaat tatataatat tatctttcct ttttgccttg gggtttgaag gttggatgaa
    10741 ttctcattat tttaaaatat ctgaaaataa ttttattact atattgtctt cattttattt
    10801 tttgagtttt cttatttttt ttattagtgt gataacaacg ttgctatatt catctgactg
    10861 gactgctctt gttattgttg gatatattca gtcgcttctt aatattacaa ctttgcattt
    10921 aagactcaag ggaaaatata taagatcagg cgcattgtta ttagcagcgt caattgttaa
    10981 tgcagtgatg gtatatgtgt gctttgagtt atttggtaaa gattataatg cgcgcattga
    11041 agctttttta ttttcttctg tgttattggt aatatttatt attctctgta acattaatat
    11101 atgggggggg gggggtaaaa ataaaattaa ttttagcttt ttaaataaag acattttgtt
    11161 attttgttta cccctgtgtt taacaatggt tattaactgg gctaagggta atatagataa
    11221 gtattttatt gctaatctac ttgatttgaa gtctcttggt atctatgctg ttgcatatca
    11281 gttagcttca gttattaatg ttgttggcat tattttgaat aggacattac aggctgattt
    11341 gcttaaaagc atggctgatg gtttttatca aaccaaaaag ataatactca tttttttatc
    11401 cgttttggct atattctttc tcctatatgt tatagctgtg tgttatggtt ttaattatgt
    11461 tttttctgcc gattatgccc cctctcgtaa tatggcgtta ctgttaatat tttgtttttt
    11521 attcttttcc acctcatcgt ttttaattaa ttttgtattg ttttacggta gacctaaatt
    11581 gattttgctt caaagtttat taatcacaac catacatgtt gcactatcgt ttcttttgat
    11641 tcagaaatac gctctgtttg gggctttatt tacacaggtt attacttcga tattaacatt
    11701 tatttccact ttattattat gctatatatt aatgaaagca tctcacaaat aacacattct
    11761 tacatgttgt aagatattta tttaatatta tcttaaatta tgaactcatt tctcaggagt
    11821 aaacaatgtc aaagcaacag atcggcgtcg tcggtatggc tgtgatgggg cgcaaccttg
    11881 cgctcaatat cgaaagccgt ggttataccg tctctatttt caaccgttcc cgtgagaaga
    11941 cggaagaagt gattgccgaa aatccaggca agaagctggt tccttactat acggtgaaag
    12001 agtttgttga atccctggaa acgcctcgtc ggatcctgtt aatggtgaaa gcaggtgcag
    12061 gcacggatgc tgctattgat tctctcaagc catacctcga taaaggtgac atcatcattg
    12121 atggcggaaa taccttcttc ctggacacca ttcgtcgtaa tcgtgagctt tctgcagaag
    12181 gctttaactt tattggtacc ggtgtctccg gtggtgaaga aggcgcgctg aaaggtcctt
    12241 ccattatgcc tggtgggcag aaagaagcct atgaactggt tgctccgatc ctgaccaaaa
    12301 tcgccgcagt ggctgaagac ggcgagccat gcgttaccta tattggtgcc gatggtgcag
    12361 gtcactatgt gaagatggtt cacaacggta ttgaatacgg cgatatgcag ctgattgctg
    12421 aagcctattc tctgcttaaa ggtggcctga atctttccaa cgaagaactg gcacagacct
    12481 ttaccgaatg gaataacggt gaactgagca gctacctgat cgacatcacc aaagatatct
    12541 tcaccaaaaa agatgaagac ggtaactacc tggttgatgt gattctggat gaagcagcaa
    12601 acaaaggtac gggcaaatgg accagccaga gtgcgctgga tctcggcgaa ccgctgtcgc
    12661 tgattaccga gtctgtgttt gcacgttata tctcttctct gaaagatcag cgcgttgccg
    12721 cgtctaaagt tctcactggc ccgaaagcgc agccagcagg cgataaggct gagttcatcg
    12781 aaaaagttcg tcgtgcgctg tatctgggca aaatcgtttc ttacgcccag ggcttctctc
    12841 agctacgcgc agcgtctgaa gagtacaact gggatctgaa ctatggcgaa atcgcgaaga
    12901 tttttcgtgc tggctgcatc atccgagcgc agttcctgca gaaaatcacc gatgcctatg
    12961 ccgaaaatcc acagatcgct aacctgctgc tggctccgta cttcaagcaa attgccgatg
    13021 actaccagca ggcgctgcgt gatgtcgttg cttatgcggt acagaacggt atccc
//
