LOCUS       AB812022               22813 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: F10167a-41.
ACCESSION   AB812022
VERSION     AB812022.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 22813)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..22813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F10167a-41"
                     /db_xref="taxon:562"
                     /note="serogroup:O60"
     gene            <1..432
                     /gene="wcaM"
     CDS             <1..432
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01049.1"
                     /translation="VVKGKYLSVPQNFRLNNITLNNSQLTFPLRGIQITSGNPVSFVA
                     LTNMELSHASLELHNQPQHLFLRNINVMQKSTIGPALTMHFDLRKDVRGMFMAKKETL
                     LSLRNIHAVNESGDNSVTIDKINQQIVNVEAINFSLPQQEK"
     gene            608..1498
                     /gene="galF"
     CDS             608..1498
                     /gene="galF"
                     /note="O060_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01050.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQAQPLGLGHSILCARPVIGDNPFVVVLPDIIIDNASADPLRYNLAAMIARFNETGRS
                     QVLAKRMAGDLSEYSVIQTKDPLDREGQVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWAELERTEPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRNSIVKLLD"
     gene            1891..3306
                     /gene="manC"
     CDS             1891..3306
                     /gene="manC"
                     /note="O060_02"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="BAQ01051.1"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLYGQRSMLQETVLRL
                     DDVDAREPVVICNQEHRFLVAEQLRQINKLSHNIILEPVGRNTAPAIALAALSAIENG
                     DDPILLVLAADHIINNKPVFHQAIKSAFKFALQGRLVTFGIVPTGPETGYGYIHRGQE
                     ETLDGQIAYQVSRFVEKPNKETAESYIDSGEYYWNSGMFMFRAKKYLEELEKFRPDIL
                     DACKAAIQGCKESDEFIKVDRDLFIACPDESVDYAVMEKTTDAVVVGLDADWSDVGSW
                     SALWEVSPKDDKGNVLTGDTFLHNANNCYINTDEKLIAAIGVDDLVIINTKDAVLVVN
                     KDQVQDVKKVVEYLKANHRSEYKRHREIYRPWGRCDVVVQTERFNVNRITVKPGAAFS
                     MQMHHHRTEHWVILSGTGEVTIKDQKFLLTENQSTFIPIGAQHRLENPGKIPLELLEI
                     QSGSYLGDDDIIRIKDQYGRC"
     gene            3326..4705
                     /gene="manB"
     CDS             3326..4705
                     /gene="manB"
                     /note="O060_03"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="BAQ01052.1"
                     /translation="MSQLTCFKAYDIRGKLGEELNEDIAYRIGRAYGEFLKPKTIVVG
                     GDVRLTSESLKLALANGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVRENAKPISGDTGLRDVQRLAEANDFAPVDQSRRGNYKQISILDAYVNHLM
                     GYIDFANFTRPLKLVVNSGNGAAGHAIDAIEKRFNDAKVPVTFIKVHHQPDGNFPNGI
                     PNPLLSECRQDTTDAVIANQADLGIAFDGDFDRCFLFDNQGQFIEGYYIVGLLAEAFL
                     QKEPGAKIIHDPRLTWNTIDIVTKAGGVPVMSKTGHAFIKERMRKEDAIYGGEMSAHH
                     YFRDFAYCDSGMIPWLLIIEMLSVTGKSLANLINEYMTAYPCSGEINFRVHNAENIVE
                     KIMNKYSNVCLNKDFADGLTLEFIDWRVNVRSSNTEPVLRVNIESRKNAQLMEEKTAE
                     IVNFIRGQE"
     gene            4705..5499
                     /gene="wzm"
     CDS             4705..5499
                     /gene="wzm"
                     /note="O060_04"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ABC transporter permease"
                     /protein_id="BAQ01053.1"
                     /translation="MFGSLIAAYKYKDFILNAIKRDFKSRYQTSMLGAAWLILQPLSM
                     ILVYTIIFSEVMKARMPDNSGAFAYSIYLCAGLLTWNFFSELISRLINIFVDNGNYLK
                     KINFPRICLPIISIGVSFLNFLIIFSLYIVFLIITGNFPGFSIFYMIPLLIIQVIFSL
                     GLGLTLGVLNVFFRDVGQFMIILIQFWFWFTPIIYLINILPEWARKWLALNPMTGLIN
                     GYQNIFVYHKTPEWSSLTLVSILAVIFTYIGYYLYRKHSGEMVDEF"
     gene            5504..6712
                     /gene="wzt"
     CDS             5504..6712
                     /gene="wzt"
                     /note="O060_05"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen export system, ATP-binding protein"
                     /protein_id="BAQ01054.1"
                     /translation="MITVNNISKIYKNYTNKKHRFIEWLTFGKKKYHKDIPILSDISF
                     NVSEGESVGILGMNGAGKSTLLKIITGTTAPTHGNISVKGRVAALLELGMGFHSDFTG
                     KENVFMSGQLMGLTSAEIDELMPDIEAFAEIGDYINQPVRTYSSGMQVRLAFAIATAV
                     RPQLLIIDEALSVGDAYFQAKCFNRIRNFKKRGTTLLIVSHDQAAIANICTRAILLDK
                     GRVVADGNPSDIMNLYNAKLSKTAEENEVKVEKGKSESGNKKAVIDSFYLEGKNGTKT
                     DIVEVGEKCSLCFNITNKSVPKGLTLGFHIRDRLGNIVFGTNTLAHDIDLASNGQQLV
                     KFTLDMNIGVGEYSISAALHKERDHYEECFHWLDGLGGFNVIAPGKIFVGSAWLNNEC
                     SVNLKRAENV"
     CDS             6705..7574
                     /note="O060_06"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="putative methyltransferase"
                     /protein_id="BAQ01055.1"
                     /translation="MFKSFKEVRNQIHYISEGISKIENLIITKFKKNSNDNETLISEL
                     KKKNEILESRLSTLESQNNELANRSITIESQLVAANAVATAIQGRLEFVREELLFEMR
                     AKNNTFKDRVEREIETKIISDEKYKDLGIRKINVGCGHIQPEGYINVDARALPGVDVT
                     CEAGALPFNDGDLEEIYSAHLIEHFTEFELLRKIIPHWKSKLTVGGILRLVTPDAVSM
                     MNDFSQGEMTFDDLRKVTYGSQDYEGDFHYNMFSVESLTNILIQAGFKRVELIEDNRK
                     NGLCREMELIAIN"
     CDS             7585..8001
                     /note="O060_07"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf8"
                     /product="hypothetical protein"
                     /protein_id="BAQ01056.1"
                     /translation="MENLDLNNINDEIISSIHYAREERALNINNIISRLSNEHKILYR
                     YKTYDINHLLSLCINDYRELITILITKKIPEDIRAFTLINRFSRRPLFFIILLAYHPD
                     AQVKINGITKIMIMKILRRNKNIFNFARKIYYKVRG"
     CDS             8004..11249
                     /note="O060_08"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="glycosyltransferase"
                     /protein_id="BAQ01057.1"
                     /translation="MYSHFKGRFIQVIESLDMNDACSNHVVHIDAFVKKKYRIKTAIY
                     AKHVHPSRAHLINFIDYLEPSDDDIIFFHFSGYSEYCASKVISANGLKILHYHNITPH
                     YFFEKNTILYNLCKKGHQQLKEIIGYFQFATADSNYNLNEIISLGFDEKRTQKLPIIL
                     DELPEKRQAVNSEENNIIFVGRICENKCQHKLIEFYHGYAKTNKIGKLFLVGKYDTSS
                     SYYKKIVRLIHTLNLEGHVFLTGPVSESQLEEYYTNSQCLISFSEHEGFGVPLLEAAQ
                     YNIPVLALNKAAVSETLEMSSGLFNTDSELTLMLQRLFSNSEYKKSILNHQQNVLANN
                     TLDAWGEYADKLFKRLLPDKERFQTISLVICTYNRGDYLDRCLDYLSKSYSDAFEVIV
                     VNGPSTDNTNDVLSRWQDKIKIRSNPERNLSRSRNIGIEAAAGDLIAFIDDDAIPFLD
                     WFDRIVNYYITSHNFVAGAGGPTYYAGTLQFQAVDIFVDNFGSGIVNPGKGIKEDPDY
                     RRSLLGTNSVFRRDYLVEAGGFDEEYDYFLDETDVCFRLINNGYLINHCPDAYLRHEF
                     AQSENRKNKYNYNWYSIVKNTVYFALTYTKGDKQEIIDELKAVIERERIDYLNSGLSN
                     KEISKSDYDDLVKSVWSGFDAGLEAIQKETKLLNSNTIKDASFAKFNEVKIANVPKHI
                     VIVTKEFPPFTRSGGIGTLYYNLASELLLAGHFVTIIMQSDKVETIENGRFRLIALTK
                     DVGSETYIDDSLIANEILNWSKRIAIEIDALNEIHPVSVVDSCLWDSEAYAFSLINKE
                     LNIPLVIRLVTPFLVANETNQWNMSSNDINYLTNFERKLVENATAVVPISDSIKKTFI
                     NKYQPSSEVEYHKINAGIAYWPKYDVASGYKELGTNLSYISEIIEDKKVFLYMGRVEL
                     RKGIDVFLDAINVINSQNNMKDVIFLIAGSDTIGIHGMIKERVSNPENIYYIGEVSDS
                     EREKLYSICDVVVFPSRYESFGLVPLEAFVHAKPVIASNAGAIPEVVIDNDSGLIFND
                     GSAEDLASRIEQLIQKPDLYSKLSLGASKRVRELSSYQSAAQSIKLYNSIG"
     gene            11269..12303
                     /gene="gmd"
     CDS             11269..12303
                     /gene="gmd"
                     /note="O060_09"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="BAQ01058.1"
                     /translation="MKSAIITGITGQDGAYLAELLLSKGYKVFGTYRRTSSVNFWRIE
                     ELGIKNNPNLHLVEYDLTDLSASIRLMQTTNASEVYNLAAQSFVGVSFDQPITTAEIT
                     GLGAVNLLEAIRIVNPKIRYYQASTSEMFGLVQAVPQKEDTPFYPRSPYGVAKLYAHW
                     MTVNYRESYNIFASSGILFNHESPLRGQEFVTRKITDSVAKIKLGKQDVLELGNMDAK
                     RDWGYAKEYVEGMWHMLQADKPDTFVLATNRTETVRDFVSMAFKAAGFTLRFEGKDEN
                     EVGIDVETGKTLVRVNPKFYRPAEVDLLIGNPARAKEILGWEPKTTLEQLCQMMVEAD
                     LRRNQQGMTF"
     CDS             12303..13205
                     /note="O060_10"
                     /codon_start=1
                     /transl_table=11
                     /gene="gmd_2"
                     /product="putative GDP-mannose 4,6-dehydratase"
                     /protein_id="BAQ01059.1"
                     /translation="MTGAGKHALITGINGFTGRYVAAELSAAGYRVFGLGAGSVPYDG
                     PDYYQVNLMDITALTNVVTSIKPNVVVHLAAIAFVGHGDADAFYNINLLGTRNLLQAL
                     SKCDSSLDAVLLASSANVYGNGTAGKLSETTAPNPANDYAVSKLAMEYMARLWMDKLP
                     VFITRPFNYTGVGQADNFLLPKIVKHFKAKAPVIELGNIDVWRDFTDVRALSQAYVKL
                     LQAKPTGEVINICSGRTYSLRKIIELCEKITGHHLEIQVNQAFVRANEVKTLSGDTAK
                     LQSFIPEWDVPPLEDTLRWMLESD"
     CDS             13205..14341
                     /note="O060_11"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf12"
                     /product="glycosyltransferase"
                     /protein_id="BAQ01060.1"
                     /translation="MKVIFGTDAIKFPLTGIGRYAFELANRLACSSEVSELLYLRGAK
                     LEKQIPVAKEIHSSTEGLKNYLKKNRLASELYRLTAPWLKTNILRKHKDAIYHGPNFY
                     LPPNVERCVVTFHDLSVFTLPQCHPPERVRYMRKELLQTINRAKLFITDSDYTRRELI
                     EYFNLSESRVFTAKLACSEDFHPRTNADIAITLKQYNLHFKQYALFTGSIEPRKNIST
                     LLDAYERLPADIRCHTPLVVSGFRGWSSDDLHVRFMKGEREGWLRYLGFTPLADLPAL
                     YAGARSFIFPSIYEGFGLPVLEAMASGVPVVCSNSSSLPEVVGDCALMCDPMDTESLT
                     RLIAKSYEDITWREWASRKGILRANEFSWTRCATETIAAYKQVY"
     CDS             14347..15462
                     /note="O060_12"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf13"
                     /product="glycosyltransferase"
                     /protein_id="BAQ01061.1"
                     /translation="MLKVLHFYKTYYPDTFGGVEQVIYQLSESGAAYGIDSTVLSLSK
                     RGDNDNKIGSHRVCYAKTNFEIASTPFSFSSIRKFAELAKEADIIHYHFPFPFMDMLH
                     FICRIKKPTVVSYHSDIVRQKSFLRLYAPLMNMFLRRVDCIVASSPNYVYTSLTLQKY
                     LHKVKVIPYGLKESSYPICKSEKLDYWRNKFGENFFLFVGTLRYYKGLHVLLEAATRP
                     ELKIVIVGSGTEEQKLKSKAKELGLSNINFVGAVTDEDKSALLTLCYCVVFPSHLRSE
                     AFGISLVEGAMHAKPLISAEIGTGTSFINVDGLTGLVVTKNSPEELRTAMLKMLADPD
                     KADAYGRNAYERYLNLFTAEKMTQEYQSVYLDIVKGN"
     CDS             15462..15863
                     /note="O060_13"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf14"
                     /product="putative membrane GtrA-like protein"
                     /protein_id="BAQ01062.1"
                     /translation="MVPCNTSCNDNKLKLKEFIKYCIVGGGNTLFTVCVIFFMMFMGF
                     GIFISNAIGYFLGIILSFVVNTKFTFNKQITIPIFFKFLVTCFFCYSINVFVIYALMH
                     IDVQNNYYIQFSGMCTYTFLSFYINKKWSMK"
     CDS             15848..16807
                     /note="O060_14"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf15"
                     /product="glycosyltransferase"
                     /protein_id="BAQ01063.1"
                     /translation="MEYEIMAGVLSIVVPCYNEQEVLDMCITELGNVLDALITKNKIS
                     SDSHILFVDDGSSDNTWKMIESYSAKNEKVKGIKLSRNKGHQLALWAGLCECRHSDIT
                     ISIDADLQDDTSVIENMVDEYNSGIHIVYGVRNDRATDTYFKRTTAKFFYKFMSLLGV
                     NQVENHADFRLLSRRALLALLNYEERNVYIRGLIPFLGFTSSKVFYSRKSRIAGESKY
                     PLKKMLALALEGITSLSIAPLRMLTLLGFSISVLSAILIIFSFIKYIMGQTLHGWTSV
                     ILAVLFIGGVQMLCIGVLGEYIGKIYIEVKKRPRYFIDKKLQR"
     CDS             16836..18836
                     /note="O060_15"
                     /codon_start=1
                     /gene="orf16"
                     /transl_table=11
                     /product="putative membrane protein"
                     /protein_id="BAQ01064.1"
                     /translation="MKLLKLLYWYAIVALTAFLFGFVHIKWVYSSYFHGDSAVMQVLG
                     QAIYDSKSILPHDFYFGNQLILLRSGLPIAIAIFLGFHGYDAFVVGSALGIVAWSLVL
                     YFSLSCAFKTKWQPFLFTFCLLFPTSFGFDSDFILGQQSHLANVILLLAALIFYVLNI
                     SSGYKRFLILSGCSFFIICVEEPIRAAFLLLTIIFISIICFDYKKYYLKLIVLFSFAL
                     LGSLCNKLLLHTHHLAVDISSSVTLIDYNAFLRNLSLVITEYFSNISSLNIYTGLPLK
                     SVGFFSYICNFTYVTIFNIYIVINIIGVCKRLYIILTGRNKPQYEIWDIIKLAAFLGL
                     IITILAICILNPDSSRHALWAIFIIKLCFLIDILKFLRGKLNFGEYISCFIMLSVILL
                     SSTWVTIATLPESFRHNKSYNADNEQLSDFIYKMSAKYKVKNIYGNDFWQTMPLNVTI
                     PGLKFAVLALSEKEYFSPMKFLMRPSYFEVSPKQKVLYYFRADDASKQFANAIKLKQG
                     QLLGQVGDFQVWLGYPLWPIFNAQYGYYKWDGCQLLTQNEVHKKDCTLIKEVNHPSSY
                     LSYGPYQELPAGTYKFSMRISSPMPAEQIIGEFDVVQGSKSILTKGALHGSNNDFVNV
                     EGLFTVDGDYLPTNKLIEVRSFINKEFSASLKNISIEKIDNR"
     gene            18953..20359
                     /gene="gnd"
     CDS             18953..20359
                     /gene="gnd"
                     /note="O060_16"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01065.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKRDEEGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKKIADDYQQALRDVVAYAVQNGIPVPTLAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRTDKDGVFHTKWIE"
     gene            20613..21779
                     /gene="ugd"
     CDS             20613..21779
                     /gene="ugd"
                     /note="O060_17"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01066.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVIALDIVPERVDILNKRISP
                     IVDSEIQHFLQSGDIRFKATLDKKIAYQNADFVIIATPTDYDPKTNYFDTSSVESVIR
                     DVIELNPDAVMVIKSTVPVGFTQAMREKFNTQNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKKDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     EALGLNSRQIIEGVCFDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYHSVPNNIISAI
                     VDANRTRKDFIADAILARKPRVVGIYRLIMKTGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMTQELFFNSRLERDLAEFKQQADVIISNRMASELLDVEDKVYTRDLFGSD"
     gene            complement(21831..22442)
                     /gene="hisI"
     CDS             complement(21831..22442)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01067.1"
                     /translation="MLTEQQRHELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     6926 a   4038 c   4826 g   7023 t
ORIGIN
        1 gttgtcaagg gtaaatattt atctgtccca caaaatttca ggctcaacaa tattaccctg
       61 aacaattcac aactgacgtt tccgttgcgt ggtattcaga ttacttcagg caatcctgtc
      121 tcctttgtcg ccctgacgaa tatggaacta agccatgcct ccctggagct gcacaatcaa
      181 ccgcaacatc tctttttacg caatattaat gtgatgcaga aatcgacaat cgggcctgcg
      241 ctaacgatgc attttgacct ccgtaaagat gtgcgaggga tgtttatggc gaaaaaagag
      301 acactactgt cgctgagaaa tattcacgca gtgaatgaaa gtggtgataa ttcagtcact
      361 attgataaaa ttaatcagca gattgtgaac gttgaagcga ttaacttctc actccctcag
      421 caagagaagt aaaaatgcga acaatcctgg taacaagcag acatacttaa gattatggct
      481 actgaaattt tatgacaggg agcgtaacat cagtagcata atcgtgcagc aattggtggc
      541 aattgatgct ggcgaaaaga gtcgaaagag ctataattca gcaaccattt tacaggtgga
      601 tgaaataatg acgaatttaa aagcagttat accggtagct ggactaggga tgcatatgtt
      661 gcctgccaca aaggcgattc ctaaagaaat gctgccgatc gtcgataagc cgatgattca
      721 gtacattgtc gacgagattg ttgctgccgg gatcaaagaa atcctcctgg ttacccatgc
      781 gtccaagaac gccgttgaaa accatttcga cacctcctat gagttagaat cattactgga
      841 acaacgcgtt aagcgccagc ttctggctga agttcagtca atctgcccgc cgggcgtgac
      901 gattatgaac gttcgccagg cgcagccgct gggcctggga cactctattt tgtgcgcgcg
      961 tccggttatc ggtgataacc cctttgtggt ggtgctgcca gatatcatca tcgacaacgc
     1021 cagcgctgac ccgctgcgct acaaccttgc cgcaatgatt gctcgcttca atgaaacagg
     1081 gcgcagtcag gtgctggcta aacgtatggc gggtgatctc tctgaatact ctgttattca
     1141 gacaaaagat ccgctcgatc gtgaaggtca agttagccgc attgttgaat ttatcgaaaa
     1201 acccgatcag ccgcagacgc tggattccga tatcatggcc gttggtcgtt atgttctttc
     1261 agcggatatc tgggctgaac tggaacgtac tgaaccaggc gcgtggggcc gtattcagtt
     1321 aactgacgcc atcgccgagc tggcgaaaaa acagtctgtt gatgccatgt tgatgaccgg
     1381 cgacagctac gattgcggca aaaaaatggg ctacatgcag gcatttgtta agtacggatt
     1441 acgcaatctc aaggaaggcg cgaagtttag aaatagtatt gtaaaacttc tcgattagtt
     1501 ccctgagttg gaattttgac attgttaagc gctatggcgg cagtttaagt tgaggctgat
     1561 gttttcccga tcgctgaaac ttactgtcgc ttttcttttt ctataaaaat cagtgactat
     1621 aacgaaacaa gcgtaaggaa atacttatcc gaaactggac attttccctt gtttacaagg
     1681 taaagaaata agagaatatg cagccagaag agaagaaagt agaaaaattg tcaatatttc
     1741 tgcgctcgga caacaacctg aaaaggcaaa aagtgacata gtgtcctggt agctgtagag
     1801 ccagtgcggt agcgtgctcg attttcagag agcatttttc acatcaactc actatttaag
     1861 cataaagcgt tgatatttta aggacttata atgttacttc ctgtgattat ggccggtggt
     1921 accggtagtc gtttatggcc gatgtcgcgc gagttatatc caaagcaatt tcttcgtctc
     1981 tatggacagc gctcaatgct gcaggagaca gtgctgcgtc ttgatgatgt tgacgcacgg
     2041 gaacccgttg tgatttgtaa tcaggaacac cgctttctgg tagcagaaca actaagacaa
     2101 attaataagc tttcccataa catcatcctt gaaccagtag gacgcaatac ggcacctgcg
     2161 attgctttag cggcgctgag tgcgattgaa aatggtgatg atcctatcct gctggtgctt
     2221 gccgccgatc atattatcaa caataaacct gtattccatc aggcgataaa atcagcattt
     2281 aaatttgccc tgcagggacg tctggtgaca tttggtattg tgcctactgg tccggaaacg
     2341 ggatatggtt atattcaccg cgggcaggaa gaaacgcttg atgggcagat cgcttatcag
     2401 gttagtcgat ttgtagaaaa accgaataaa gaaacagcag agtcctacat tgactctggt
     2461 gagtattact ggaacagcgg aatgttcatg ttccgggcaa aaaaatacct cgaagaactt
     2521 gaaaaattca gaccagatat tctcgatgcc tgtaaagcgg ctattcaggg ttgcaaagaa
     2581 agcgatgagt ttatcaaagt cgatcgcgat ctgttcattg cttgtccgga tgagtcggtg
     2641 gattacgccg tcatggaaaa aaccactgat gccgtcgtcg ttggacttga tgcagactgg
     2701 agtgatgtgg ggtcctggtc tgccctgtgg gaagtcagcc cgaaagatga taaaggcaat
     2761 gtgctgaccg gcgacacctt cctgcataac gccaacaact gctacatcaa taccgatgaa
     2821 aaactcatcg cggcaattgg tgttgatgat ctggtgatta ttaatactaa agatgctgtg
     2881 ctggtggtga ataaagatca ggtgcaggat gttaagaaag tcgttgaata tctgaaagct
     2941 aatcaccgca gtgaatacaa acgccaccgt gagatatatc gtccgtgggg ccggtgtgat
     3001 gtggttgtgc aaaccgaacg ttttaacgtc aatcgcatta cggttaaacc gggtgctgcg
     3061 ttttcaatgc agatgcatca tcaccgtacc gaacactggg ttattctctc cggaacaggt
     3121 gaggtaacca ttaaagatca gaaattcctg ttaacggaaa atcaatcgac ctttatccca
     3181 ataggcgctc aacaccgact ggaaaaccct ggcaaaatcc cattggaatt gcttgaaatc
     3241 caatcaggtt cctatttagg ggacgatgat attatccgta ttaaagacca atatggccgt
     3301 tgctaacagt aaaggtaaaa gatttatgtc acaactgact tgctttaaag cctatgatat
     3361 tcgtggaaaa ttaggcgaag aattaaatga agatattgcc tatcgtattg gtcgcgctta
     3421 tggggaattt ctcaagccaa aaacaattgt tgttggtggc gatgtccgtt taaccagcga
     3481 aagcctgaaa ctggcactgg caaatggcct gatggatgca ggtactgacg tgctggatat
     3541 tgggctaagc ggcacggaag agatctattt tgccactttc cacctgggcg tggatggtgg
     3601 tattgaagtt accgcaagtc ataacccgat ggattacaac gggatgaagc tggttcgtga
     3661 aaatgccaaa ccgattagcg gtgatacagg attacgcgat gtacagcgtc tggctgaagc
     3721 caatgatttt gcgcctgtcg atcaatcccg tcgcggaaat tataagcaga tctccatatt
     3781 agacgcctat gttaatcacc tgatgggcta tatcgacttt gcaaacttta cccgcccgct
     3841 aaagctggtt gtcaattccg gtaatggtgc cgcaggtcat gccattgacg ctattgaaaa
     3901 gcgtttcaac gatgcgaaag tacctgtaac ttttattaag gtacatcacc aaccagatgg
     3961 taatttcccc aatggtattc ctaatccact gctgtcagag tgccgccagg ataccacgga
     4021 tgcagtgatt gccaatcagg cagatctggg tattgcattt gacggcgact ttgaccgctg
     4081 cttcctgttc gacaatcagg ggcaatttat tgaaggttac tacattgttg gtctgctggc
     4141 ggaagcattc ttacagaaag aacccggtgc aaaaatcatt cacgatccgc gtcttacctg
     4201 gaacacaatt gatattgtga ctaaagccgg tggtgttccg gtaatgtcga aaacgggaca
     4261 tgcgtttatt aaagaacgca tgcgtaaaga agatgctatt tatggcggag aaatgagcgc
     4321 acatcactat ttccgcgatt ttgcttattg tgatagtggg atgatcccgt ggttgcttat
     4381 catagaaatg ctatccgtga ctggtaaaag cttagcaaat ttgataaatg aatatatgac
     4441 tgcatatccg tgcagcggag aaatcaattt ccgagtacat aatgcagaaa atattgtaga
     4501 aaagattatg aataaatatt caaatgtatg tttgaataaa gattttgccg atgggttaac
     4561 attagaattt attgattgga gagttaatgt tagatcttca aatactgaac ctgtattgag
     4621 agtaaatata gaaagccgca aaaacgcaca attaatggaa gagaaaacag ctgagattgt
     4681 gaattttatc cggggtcagg aataatgttt ggttcattaa ttgcagcata taaatataaa
     4741 gatttcattc tgaatgccat caagcgagat ttcaaaagta ggtatcaaac aagtatgcta
     4801 ggtgcggcat ggctaatatt gcagcctctg tcaatgatac ttgtttatac tataattttc
     4861 tccgaagtga tgaaagcaag gatgcctgat aattccggcg cgtttgccta tagtatttat
     4921 ttatgtgcag gccttcttac atggaatttt ttttcggaat taataagccg acttattaat
     4981 atattcgtcg ataacgggaa ttatttaaag aaaattaatt tcccaagaat ctgtttgcct
     5041 atcataagta tcggtgtttc atttcttaat ttcttgataa tattttcact ttatattgtc
     5101 tttttgatca taacgggcaa ttttcccggt ttttctattt tttatatgat ccctctactg
     5161 ataattcaag tgattttttc gcttggttta ggcttaactt taggtgtgtt aaacgttttc
     5221 tttcgtgatg ttggccaatt catgattata ttgatacagt tttggttttg gttcactccc
     5281 atcatctatt taattaatat ccttcctgaa tgggctagaa aatggttagc tttgaatcca
     5341 atgacggggc taataaatgg ctatcaaaat atatttgttt accataaaac tcccgaatgg
     5401 tcttcactta ctttggtgtc aatattagct gtaatattta catatattgg ctattatctt
     5461 tatcgcaaac attctggcga gatggtagat gagttctgat gcaatgatta cagtaaataa
     5521 tattagtaag atctataaaa attataccaa taaaaaacat cggtttattg agtggctaac
     5581 atttggtaaa aaaaagtatc ataaagatat accaatatta tctgatatat catttaacgt
     5641 atctgaaggt gagtcagtag gtatccttgg aatgaatggg gcaggtaaaa gtacgttgtt
     5701 aaagattata acgggtacga ctgcaccaac tcatggcaat ataagcgtta aaggccgcgt
     5761 ggcggcattg cttgaacttg gcatggggtt ccattctgat tttactggaa aagaaaatgt
     5821 ctttatgtca gggcagttaa tgggattaac ctccgcagaa atagatgaat taatgcccga
     5881 catcgaagca tttgcagaaa ttggagatta cattaaccaa ccggttagaa catattcgtc
     5941 gggaatgcaa gtcaggctgg cattcgcaat agccacagca gttcgtccgc aattattgat
     6001 tattgatgag gcattgtcgg taggtgatgc ttattttcag gctaaatgct tcaatcggat
     6061 aagaaatttt aagaaaaggg ggacgacttt attaattgta tctcatgatc aagctgcaat
     6121 tgcaaacatc tgtaccagag caattttatt agataaggga agagttgttg ctgatggtaa
     6181 tccttcagat ataatgaatt tatataatgc aaaattatca aaaacagctg aagaaaatga
     6241 agttaaagtt gagaaaggga aatcggaatc tggcaacaag aaagctgtga tagatagttt
     6301 ctacctcgag gggaaaaatg gcacaaaaac agatattgtt gaagttggcg aaaaatgttc
     6361 actatgtttc aatataacta ataaaagtgt tccgaaaggt ttaacattag gttttcatat
     6421 cagggatcgt ttaggtaata ttgtatttgg aacgaatacc ttagcccatg atattgatct
     6481 tgcttcgaac ggacaacaat tagttaagtt cacgcttgat atgaatatag gggtcggcga
     6541 atatagcata tcagctgctt tacataaaga aagagatcac tacgaagaat gctttcattg
     6601 gctggatggt ttaggtggtt ttaatgttat tgcacctgga aaaatttttg taggaagtgc
     6661 ctggttaaat aacgaatgtt ccgtcaattt aaagcgagct gaaaatgttt aaatcattta
     6721 aagaagtaag aaaccagatt cattatattt cagaaggtat ttcgaaaatt gaaaacctga
     6781 ttattactaa atttaaaaaa aatagtaatg ataatgaaac cttgatttct gaattaaaaa
     6841 agaaaaacga aatacttgag tcaagactaa gtactctcga atcacaaaat aatgaattgg
     6901 caaatcgctc aataacaatt gaatcacagc tggtggcagc aaatgccgta gccacggcca
     6961 tacaaggaag gctcgagttt gtaagagaag aattgttatt tgaaatgcgc gcaaaaaata
     7021 ataccttcaa agatagagtt gaacgtgaaa tagaaacaaa aatcatctct gatgaaaaat
     7081 acaaagatct tggtatacgc aaaattaatg taggatgcgg tcatatacag ccagaaggat
     7141 atattaatgt agatgcacgg gctttgccgg gggttgatgt tacttgtgag gcaggggcat
     7201 taccttttaa tgatggagac ctggaagaga tttattctgc acatctaatt gaacacttta
     7261 ctgaatttga gcttttaagg aaaataattc cacattggaa atcgaaactt actgttggtg
     7321 ggattctgag gcttgtaact cctgatgctg tcagcatgat gaatgatttt tctcagggtg
     7381 agatgacttt tgatgatttg cgaaaggtta cttacggctc gcaagattat gaaggcgatt
     7441 ttcattataa tatgttttca gttgagtcat taacgaatat tttaatccag gctggattta
     7501 aaagagtcga acttattgag gataatcgga aaaatggact ttgtcgggaa atggaactta
     7561 ttgcgataaa ttagagaata tacaatggaa aatttagatt taaacaacat taacgatgaa
     7621 ataatatcgt caattcatta tgccagagaa gaaagagcat taaatataaa taacataata
     7681 tcgaggttgt ctaacgaaca taaaattctc tatagataca aaacttatga tataaatcat
     7741 ttattatctt tatgtataaa tgattataga gaattaatta caattttgat aactaagaaa
     7801 atacctgaag atattcgagc ctttacgctg atcaatcgat tcagtagaag acccttattc
     7861 ttcatcattc ttcttgcata tcatcctgat gcccaggtta aaattaatgg tattactaaa
     7921 ataatgatta tgaaaatctt aagaagaaat aaaaacatat ttaattttgc cagaaaaata
     7981 tattacaaag tgagaggctg aatatgtata gccattttaa gggtaggttt atccaggtta
     8041 ttgaatcttt agatatgaac gacgcatgtt ccaatcatgt tgtacatata gatgcattcg
     8101 tcaagaagaa atatagaata aagactgcta tatacgcgaa acatgtgcat ccttctcgtg
     8161 ctcacttgat aaattttatt gactatttag aaccatctga tgatgatata attttttttc
     8221 atttttctgg atattcagag tattgcgcaa gcaaggtgat ttctgcaaac ggcttaaaaa
     8281 tccttcacta tcataatatt acaccgcatt atttctttga aaaaaatact attctttaca
     8341 atttatgcaa gaaaggacat cagcagttaa aagaaattat tggctatttt caattcgcaa
     8401 ctgcggatag taactataat ttaaacgaaa taatttcttt aggttttgat gaaaaaagaa
     8461 cacaaaaact tccaattata ttagatgaac tacctgaaaa gagacaagca gtaaacagtg
     8521 aagaaaacaa tattatcttt gtagggcgta tttgtgaaaa taagtgtcag cataaattaa
     8581 ttgagtttta tcatggctat gccaagacta ataagatcgg aaagttattt ttggttggaa
     8641 aatatgacac atcatcttcc tattataaaa aaatagtccg tcttatccat acacttaatc
     8701 ttgaaggtca tgtttttctt actggtcctg tatctgaatc tcaattagag gaatattaca
     8761 cgaattcaca atgcttgatt tcttttagtg agcatgaagg ttttggtgtt cctcttcttg
     8821 aggcagcaca atacaacata cctgtactgg cattaaataa agctgcggtt tcagagacgt
     8881 tagaaatgtc tagcggtctt tttaataccg acagtgaatt aactttaatg ctacagcgct
     8941 tatttagtaa ttctgaatat aaaaaatcta tattaaacca ccaacaaaac gtactcgcaa
     9001 ataatacgct tgatgcttgg ggggagtatg cagataaatt atttaaaaga ttgctccctg
     9061 ataaagaaag attccaaaca atatctcttg taatttgtac ctataatcgg ggtgattatt
     9121 tagatagatg tttggattat ttgtcaaaaa gctatagtga tgcttttgaa gttattgtgg
     9181 ttaatggacc ctctacagat aatacaaatg atgtccttag ccgttggcaa gataagataa
     9241 aaattagatc aaacccagaa agaaatcttt cacgttccag gaatataggg attgaggcag
     9301 cagcagggga cttaattgca tttattgatg atgatgcaat accattcctg gactggtttg
     9361 atcggattgt aaattactat ataacatcac ataattttgt tgcaggagct ggtggtccaa
     9421 catattatgc tgggacatta caatttcagg cagtagatat ttttgtagat aattttggaa
     9481 gtggcatcgt aaacccaggt aaaggaataa aagaagatcc agattataga agaagtttgt
     9541 taggaactaa ttctgttttc aggcgcgact atttagtcga ggctggagga tttgatgaag
     9601 aatatgatta cttcttagat gaaacagatg tttgctttag gcttataaat aacggatatt
     9661 tgataaacca ttgtcctgat gcttatctcc ggcacgaatt tgcgcaaagt gaaaacagga
     9721 aaaataaata taattataat tggtactcta ttgttaaaaa tactgtttat tttgcgttaa
     9781 catatactaa aggggataag caagaaataa ttgatgagct taaggctgta attgaacggg
     9841 aaagaattga ttatttaaat agtggtttat cgaataaaga aatatcaaaa agtgattacg
     9901 atgatctggt taaatctgtt tggagcggtt ttgatgctgg tttagaagcc attcaaaaag
     9961 aaactaaatt gttaaatagc aataccatta aagatgctag ctttgctaaa tttaacgaag
    10021 ttaaaatagc taatgtccct aaacatattg ttattgttac aaaagaattc ccaccattta
    10081 cgagatcagg tggtattggg acattatatt ataatttagc cagtgaatta cttttagctg
    10141 gtcattttgt tacaataata atgcaaagtg ataaagtaga aacaattgaa aatggtaggt
    10201 ttagacttat tgctttaaca aaagatgtag gttctgaaac atatatcgat gattctctta
    10261 tcgcaaatga aatcttaaac tggtctaaaa gaattgccat agaaattgat gcattaaatg
    10321 aaatacatcc tgtttctgtt gttgattcat gtctttggga ttctgaggca tatgctttta
    10381 gtttaattaa taaagaatta aatataccat tggtaattcg tttggttact ccttttttag
    10441 tagcgaatga aacaaatcaa tggaacatga gttcgaatga tattaattat ttaactaatt
    10501 ttgagaggaa acttgttgag aatgctactg ctgtagtgcc aattagtgac tccataaaga
    10561 aaacctttat aaataaatat cagccatcat cagaagtgga atatcacaaa attaatgctg
    10621 gtatcgcata ttggcctaaa tatgatgttg ctagtgggta taaggagctt ggcacaaatc
    10681 tttcatatat cagtgaaatt atagaagaca aaaaggtttt tctgtatatg ggccgtgttg
    10741 aactccgaaa aggaattgat gtttttttag atgctattaa tgttattaat agccagaata
    10801 acatgaaaga tgtcattttc ttaattgctg ggagtgatac tattggtata catggtatga
    10861 ttaaggaacg ggtgtctaat cctgaaaaca tttattatat aggagaggtt agcgattctg
    10921 aacgagaaaa attatattct atatgtgatg ttgtcgtgtt cccttccaga tatgaatcat
    10981 ttggattagt acctttagaa gctttcgtgc atgccaaacc tgttattgca agtaacgcag
    11041 gtgcaatccc tgaagtggtt atagataacg attccggtct tattttcaat gatggatcag
    11101 ctgaggattt agcttcccgt atagaacagt tgattcaaaa acctgacctt tatagtaagt
    11161 tgagtcttgg tgctagtaag cgagtacgtg aactctcatc ctatcaatca gcggcacaat
    11221 caattaaact ttataattct attggttaat caaaggtaag tagaagttat gaaatctgca
    11281 atcattacag gtataacggg tcaagatggt gcttatcttg ctgaacttct tctcagcaaa
    11341 gggtataaag tatttggtac ataccgtcgt accagttctg tcaatttctg gcgtatcgaa
    11401 gaactcggta taaaaaataa ccccaatttg catcttgtag aatatgatct taccgatctt
    11461 tctgccagca ttcgtctcat gcaaacgaca aacgcttcag aagtttacaa ccttgcggca
    11521 caaagcttcg ttggtgtatc ttttgaccaa ccgatcacta cagctgagat cacaggactt
    11581 ggtgctgtaa atctgctgga agctattcgt attgttaatc caaagattcg ctattaccaa
    11641 gcttcaactt cggaaatgtt tggacttgta caagccgtgc cgcaaaaaga agatacacca
    11701 ttctatccac gcagtccgta tggtgttgct aaactgtatg ctcactggat gacagtaaac
    11761 taccgtgaga gctataacat ttttgcttcc agcggtatcc tgtttaatca tgaatctccc
    11821 ttacgtggtc aggagtttgt aacacgtaaa attacagatt ccgtagcaaa aattaagctt
    11881 ggtaagcaag atgttctgga actagggaac atggatgcca agcgcgattg gggttatgca
    11941 aaagaatatg ttgaaggtat gtggcatatg ctgcaggctg ataagcccga tacctttgtt
    12001 ctggcaacaa atagaaccga aaccgttcgt gactttgtca gcatggcttt caaagctgct
    12061 gggtttaccc ttcgctttga aggtaaagat gagaatgaag taggtattga tgtcgagact
    12121 ggtaaaactt tggtccgcgt taatccgaaa ttctatcgtc ctgctgaggt tgatctcttg
    12181 attggtaacc ctgcgcgagc gaaagaaatt cttggttggg aaccaaaaac tacccttgag
    12241 cagctttgcc agatgatggt agaggctgat ctacgtcgta accaacaggg aatgactttc
    12301 tgatgactgg agccggaaaa catgccttaa ttacagggat caatgggttt accggccgtt
    12361 atgtggcagc ggaactttcc gctgccggat atcgcgtttt cgggctagga gcagggtctg
    12421 tcccttatga cggacctgat tattatcagg taaatctgat ggatattact gcattaacca
    12481 atgtcgttac ctcgataaaa cccaatgtcg tcgtacacct ggccgccatc gcttttgtag
    12541 gtcacggtga tgcggatgca ttttataata tcaacttgct tggcacccgt aatttattgc
    12601 aggcactgag taagtgcgat agctcattag acgcggtact tcttgccagt agtgcgaatg
    12661 tttatggaaa tggtactgcc ggaaagttaa gtgaaacaac ggcacctaat ccggcaaatg
    12721 actatgcagt tagtaaattg gcgatggaat atatggctcg cctatggatg gataaactac
    12781 cggtatttat taccaggcct tttaactata caggtgttgg tcaggctgat aattttttat
    12841 tgcctaaaat tgttaaacat tttaaagcca aagctccggt gattgagctt ggtaatattg
    12901 atgtgtggcg agatttcaca gatgtacgtg cgttaagtca ggcttatgtg aagctacttc
    12961 aggcaaaacc cactggagaa gtgataaaca tttgctcagg gcgtacctac tcactgagaa
    13021 aaattattga attgtgtgaa aaaatcactg gtcatcacct tgagattcag gttaaccaag
    13081 cctttgtgcg tgcaaatgaa gttaaaacgt taagtggaga taccgctaag cttcagtctt
    13141 ttatacctga gtgggatgtt ccaccccttg aagatacctt acgttggatg ttggaaagtg
    13201 actaatgaaa gtaatatttg gaactgatgc gatcaaattt ccattaacag gcatcggtcg
    13261 ttacgcattc gagttggcta accgtttagc ttgcagttcg gaagtatccg aactgcttta
    13321 tctaaggggg gcaaaactgg aaaaacaaat ccctgttgct aaagaaatac attcttccac
    13381 agagggttta aaaaattatt taaagaagaa taggcttgcg tctgaattat atcgattaac
    13441 ggcaccatgg ttaaaaacga acattctcag aaaacataaa gatgcaattt accatggccc
    13501 taatttttat cttccaccaa atgttgaacg atgtgttgta acatttcatg atctttcagt
    13561 atttacttta cctcaatgtc atccccccga acgtgttaga tatatgcgta aggaattatt
    13621 gcagactata aatcgtgcaa aactttttat tacggactcg gactatactc gaagagagct
    13681 gattgaatat ttcaacctct ctgaaagcag agtttttaca gcgaaacttg cttgcagtga
    13741 agactttcac ccacgcacta atgctgatat agccatcact cttaagcaat ataatttaca
    13801 tttcaaacaa tacgctcttt tcactggatc aattgaaccc cgcaaaaata tttctactct
    13861 attagatgca tacgagcgcc ttcctgcaga tatccgatgc catacgccgc ttgtggtaag
    13921 tggatttaga ggttggagta gtgatgatct ccatgttcgt tttatgaagg gggaaaggga
    13981 aggatggctt cgatatcttg gttttactcc attagctgac ttgcctgctt tatatgcagg
    14041 tgccaggagt tttatctttc catcgattta tgaaggtttt ggacttcctg tacttgaagc
    14101 aatggcttca ggtgtacctg tcgtttgctc aaatagttca tctctcccag aagttgttgg
    14161 tgattgtgcg ttaatgtgtg atccaatgga tacagaatca cttaccaggc tgatagcaaa
    14221 aagttatgaa gatattacct ggcgagaatg ggctagcaga aaagggatac tacgtgctaa
    14281 tgaattctca tggactcgat gtgccactga aaccatagca gcatataaac aggtttacta
    14341 acaacaatgt taaaagtatt acatttttat aaaacatatt atcctgatac atttggtggg
    14401 gtagagcagg taatttacca gctaagtgag tcgggagctg cgtacgggat tgattcaacg
    14461 gtactttcac tgagtaaacg tggtgataat gataataaaa ttggatcaca tcgagtgtgc
    14521 tatgctaaaa cgaacttcga aatagcttca acgcctttct ctttttcaag tattcgaaaa
    14581 tttgctgaat tagcaaaaga agcagatatc atacattacc atttcccttt tccatttatg
    14641 gatatgcttc actttatctg tagaataaaa aaacctacgg ttgttagcta tcactctgat
    14701 attgtcagac agaaatcatt tttaagatta tatgcgccac taatgaatat gtttctaaga
    14761 cgagttgatt gtatagtggc atcatctcct aattatgtat ataccagtct gacattgcaa
    14821 aaatatttac ataaagttaa agtaattccc tatgggttaa aagaatcatc ctacccgatt
    14881 tgtaaatctg aaaagctaga ttattggaga aataaattcg gagaaaattt ttttcttttt
    14941 gttggaactt tgagatatta taaaggtctt catgttttat tagaagcagc aacaagacca
    15001 gaattaaaaa tcgttattgt tggttcggga actgaagagc agaagttaaa aagcaaagca
    15061 aaagaattag gacttagtaa tataaatttt gtcggtgccg taacggacga agataagtca
    15121 gcactgttga cgttatgtta ttgtgtagtt ttcccttccc atttgagatc tgaagctttt
    15181 ggaatttctt tagtcgaggg agctatgcat gcaaaaccat taatttctgc agaaataggc
    15241 actggtactt catttattaa tgttgatggt ctcactggac ttgtcgttac taaaaatagt
    15301 cctgaagaat tacgaacagc aatgttgaaa atgcttgcgg atcctgataa ggcagatgct
    15361 tatggcagaa atgcatatga aagatacttg aatcttttta cggcggaaaa aatgacacaa
    15421 gagtatcaat ctgtttattt ggacattgta aaaggtaatt aatggtgcct tgtaatacta
    15481 gttgtaacga caataaattg aaattaaaag aatttataaa gtattgcatc gtcggtggtg
    15541 gaaatacttt atttacagta tgcgttatat tttttatgat gtttatggga tttggtattt
    15601 tcatatctaa cgcaatagga tattttttag gaataatact gagcttcgta gttaatacta
    15661 agtttacttt taataaacaa atcacaattc ctattttttt taaatttttg gtgacatgtt
    15721 ttttttgcta ttccataaac gtttttgtga tatatgcatt aatgcatata gatgtacaaa
    15781 ataactatta cattcaattt tcaggaatgt gtacatatac atttttaagc ttttacataa
    15841 ataagaaatg gagtatgaaa taatggcagg tgtattatcg atagttgtgc cctgttacaa
    15901 cgaacaagaa gtattggata tgtgtataac cgaattaggg aatgtgttag atgcattaat
    15961 aacaaaaaat aaaataagtt ctgatagtca cattcttttt gttgatgatg gtagttctga
    16021 taatacctgg aaaatgattg aatcttattc ggctaagaat gaaaaagtta aagggattaa
    16081 attatcaaga aataaaggcc accaacttgc tctttgggcg ggtttatgtg aatgcagaca
    16141 ctctgacatt acaataagta ttgatgctga cttacaagac gatacatcag ttatagaaaa
    16201 tatggttgac gaatacaatt caggtatcca tattgtctat ggtgtaagaa atgatcgtgc
    16261 aacagatact tattttaaaa gaacaacagc aaaatttttc tacaagttta tgtctctact
    16321 tggcgtaaat caggttgaaa atcatgctga tttcagattg ttaagtcgac gcgcacttct
    16381 ggcattactc aattatgaag agaggaacgt gtacatccgc ggacttatac ctttccttgg
    16441 gtttacttca tcaaaagtat tctatagtag aaaatcacgt attgcgggtg aatctaaata
    16501 tccgttaaaa aaaatgttag cattagcact cgaggggata acatctcttt ctattgcccc
    16561 attaagaatg ttaactttat tagggttttc gatatcagtt cttagcgcaa tattaattat
    16621 tttttctttc attaaatata ttatggggca aactctgcat ggttggacat ctgtaattct
    16681 cgcggtgcta tttattgggg gagtacaaat gctttgcatc ggtgttttgg gcgaatatat
    16741 tgggaaaata tacattgaag taaaaaaacg tcccagatat tttattgata aaaaacttca
    16801 aagatagaat gattaaataa aggattttaa actcaatgaa attgttaaaa ttattatatt
    16861 ggtacgcaat tgttgcgtta acggcttttt tgttcggatt tgttcatatt aaatgggttt
    16921 attcatctta ctttcatggc gactcagctg ttatgcaagt tctggggcag gcaatttatg
    16981 attctaaatc aatcttgcca catgattttt attttggcaa tcaattgata ttgttaagat
    17041 cgggcctccc tattgctatt gctatatttt tggggttcca tggttatgat gcttttgttg
    17101 ttggatctgc attaggtata gtagcctggt ctcttgtatt atattttagt ttgtcttgtg
    17161 cttttaagac taaatggcaa cctttccttt ttacattttg tttgttgttt ccaacaagct
    17221 ttggatttga cagtgatttc atattagggc aacagtctca tttggcaaat gttatattat
    17281 tattagcagc gcttatattc tatgtcctaa atatttcaag tggctataaa cgttttctaa
    17341 ttctctcagg gtgttctttt tttatcatct gtgttgaaga accaatacga gcagcttttt
    17401 tactattaac tataatattt ataagtatta tatgttttga ttacaaaaag tattatctga
    17461 aactcatagt actattttct tttgctctct tgggttcatt atgtaataag ttattattac
    17521 acactcacca tctcgcggtt gatatttcat ctagtgtaac tctaattgac tataatgctt
    17581 ttttaagaaa tttatccctt gttattactg aatatttttc aaatatttca tcacttaaca
    17641 tttacactgg cctgccattg aaaagcgtgg gttttttttc ttatatatgc aactttactt
    17701 atgttacaat atttaatatt tatattgtca ttaatataat tggcgtctgt aaaagattat
    17761 atatcatctt gacaggaaga aataaaccac aatatgaaat ttgggatata ataaagttag
    17821 ctgctttttt aggtttaatt attacgattt tggcaatatg tattttaaat ccagattcat
    17881 ctcgccatgc tctttgggct atatttatta taaaattatg ttttttaatt gatattctga
    17941 aatttctgcg aggaaaatta aattttggag aatatatctc ctgtttcatc atgttatctg
    18001 taattctatt atcgtctaca tgggttacaa tagcaacttt gcccgaatca ttccgacata
    18061 ataaaagtta taatgctgat aatgaacagt tgagtgattt tatatacaaa atgtcagcga
    18121 aatataaagt taaaaatatt tatgggaatg atttttggca aacaatgcca ttaaacgtta
    18181 ccattcccgg gttgaagttt gctgttctgg ccttgagtga aaaggaatat ttttcgccca
    18241 tgaagttttt aatgcgacca agttattttg aggtttcgcc aaaacagaaa gttctctatt
    18301 attttagagc agatgatgcc agtaagcagt ttgcaaatgc tattaaactt aagcaggggc
    18361 agttgcttgg tcaggttggt gattttcagg tttggttggg ttatcctctg tggccaattt
    18421 ttaatgctca atatggttat tataagtggg atggatgcca attacttaca cagaatgaag
    18481 ttcataaaaa agattgtacg ttgattaaag aagtaaatca tccttcgagt tatttgtctt
    18541 acggacctta tcaggaattg ccagcaggaa catacaaatt ttcaatgcgt attagtagtc
    18601 caatgcccgc tgagcaaata ataggtgagt ttgatgttgt gcagggtagt aagtcaatac
    18661 tcactaaagg tgctttacac ggaagtaata atgattttgt aaatgtcgaa ggcttattta
    18721 cagttgatgg tgattatttg cccacaaata aactaataga agtaaggtca tttatcaata
    18781 aagaattctc tgcatcatta aagaatatta gtatcgaaaa gattgataat agataaaaat
    18841 aatcccaaac tgcatcttat tggttaatat ataattaact tgtgttgcgc agtatgttaa
    18901 tatattattt acaacaaatg atttaaagct atacagaatg caggagtaaa aaatgtccaa
    18961 gcaacagatt ggcgtcgtcg gtatggccgt gatggggcgc aaccttgcgc tcaacattga
    19021 gagccgtggt tacactgtct ccgttttcaa ccgctctcgc gaaaagaccg aagaagtcat
    19081 tgctgagaat ccaggtaaga aattggttcc ttattatacg gttaaagagt ttgttgagtc
    19141 cctcgaaacg cctcgccgca tcctgttaat ggtgaaggcc ggggctggga cagacgctgc
    19201 tatcgattct ctgaaacctt atcttgataa gggcgatatc attattgatg gtggtaacac
    19261 tttcttccag gacaccatcc gtcgcaatcg tgagctttct gcggaaggct ttaactttat
    19321 cgggactggt gtttccggtg gtgaagaggg cgctttgaaa ggtccttcta ttatgccggg
    19381 tggtcagaaa gaggcctatg agctggttgc tcctattctg actaaaatag ccgctgtagc
    19441 cgaagacggt gaaccatgcg ttacctacat cggtgcggat ggcgcgggtc attatgtgaa
    19501 aatggttcac aatggtattg aatatggcga tatgcagttg attgcagaag cttattcttt
    19561 gctgaaaggt ggcctgaatc tgagcaatga agaactggca cagaccttta ctgaatggaa
    19621 caatggtgaa ctgagcagct acctcatcga cattaccaaa gacatcttca ccaaaagaga
    19681 tgaagagggt aattatctgg ttgatgtcat tctcgatgaa gccgctaaca aagggactgg
    19741 caaatggacc agccagagcg ctctggatct cggcgagccg ctgtcgttga ttacagagtc
    19801 tgtgttcgcc cgctacatct cttctcttaa agatcagcgc gttgccgcgt ctaaagtact
    19861 ctccggcccg caagcgcagc cagcaggcga taaagctgaa ttcatcgaga aggttcgtcg
    19921 cgcgttatac ttgggcaaaa tcgtttccta tgctcagggc ttctcacaac tgcgtgcggc
    19981 atctgaagag tacaactggg atctgaacta cggcgagatc gccaaaattt tccgtgcagg
    20041 ctgcattatc cgtgctcagt tcctgcagaa aattactgat gcttatgctg aaaatccgca
    20101 gattgctaac ctgctgctgg caccgtactt taagaaaatt gcggacgact accagcaggc
    20161 tctgcgtgat gtcgtggctt atgcggttca gaatggtatt ccggtaccaa ctttagctgc
    20221 cgcagtggcg tattatgata gttatcgcgc tgccgttctc cctgccaacc tgattcaggc
    20281 gcagcgtgac tattttggtg cgcatactta taaacgtact gataaagatg gcgtattcca
    20341 tacgaaatgg attgaataat tccgaataaa taatctgaaa aatacaaaaa aggcccggat
    20401 ttaatcatcc gggccttttt ttattactaa tctgattgat tttatcctgc tccttaatat
    20461 tatcttaatc cctgactgtt aacctgataa agaaaatctg aattgttttc tgcgtcgttg
    20521 atcattgttc atctaagcgc taaaactttt aacttctcgt cgggatgtta aaacatcatg
    20581 attaattagt aagataattc tgagaacatg taatgaaaat caccatttcc ggtaccggat
    20641 atgttggcct gtctaacggt ctgcttatcg cgcaaaacca tgaagttata gcattagata
    20701 ttgtcccaga gcgcgttgac atattaaata agcggatctc tcctattgtg gatagcgaaa
    20761 ttcagcattt tttacaatca ggtgatatac gatttaaagc gacattagat aaaaaaattg
    20821 cctaccagaa tgctgatttt gtgattattg cgacacctac tgattatgat cctaaaacca
    20881 actatttcga cacctcaagc gttgagtcgg ttatccggga tgtcattgaa ctgaaccctg
    20941 atgcggtcat ggtcatcaag tccacggtgc cggttggttt tacgcaagcc atgcgagaga
    21001 agtttaatac ccagaatctc atcttctccc cggagttcct gcgggaaggt aaagcccttt
    21061 acgacaacct tcacccttcc agaattgtga tcggcgagcg ttctgaacgc gcagagcgtt
    21121 tcgccgcgct attacaggaa ggcgccatta agaaagatat ccctacgtta tttacggact
    21181 ccaccgaggc agaggccatt aagctttttg ccaataccta tctggcgatg agagtggctt
    21241 attttaacga gctcgatagc tacgctgaag ccctgggatt aaattcccgt cagataattg
    21301 aaggtgtctg tttcgacccg cgtatcggta atcactataa caatccttcc tttggctatg
    21361 gcggatattg tttaccaaaa gatacgaagc aactgctcgc caattaccac tcagtaccaa
    21421 acaatattat ttcagctatc gtcgatgcta accgtacacg taaagacttt attgccgatg
    21481 ctattttggc gagaaaacca cgtgtggtgg gtatttaccg actgataatg aaaacgggtt
    21541 cagataactt ccgtgcttcc tcaattcagg gaattatgaa gcgtatcaag gcgaaaggtg
    21601 tggaagtcat catctatgaa cctgtcatga cgcaagagct attttttaac tcccgcctgg
    21661 aacgcgatct cgcggaattt aaacaacagg ctgatgtcat tatctcaaat cgcatggcga
    21721 gcgaactgct cgatgttgaa gataaggtct atacacgcga tctgtttggc agtgactaag
    21781 agcggatgaa gagtacaaat cgggctaaat aatagcccga tttgcggtga ttattgatga
    21841 cgcttacgca agttctctat caccgttgtt aaatccagat cctgatcctg caacagcacc
    21901 agcaggtgat acatcaagtc agatgcctcg ttggtcagtt caaagcggtc atgtaccgtc
    21961 gctgccagcg cagtttccac gccttcttcg cccactttct gcgcaatgcg cttggtgccg
    22021 ctggcataca gtttggcggt gtaagaggtt tccgggtcgg cagatttgcg ttcggcgagc
    22081 agttgttcca gttgatacag gaacagccac tgatgagcgg tgtcgccgaa gcagctgctg
    22141 gtgcctttgt ggcaggttgg cccgatggga ttcgccagca ccagtaacgt gtcgttgtcg
    22201 cagtccgggg caatactcac cacattgagg aaattgccag acgtttcgcc tttggtccac
    22261 agccgttgtt tagtgcgcga gaagaaggtg actttgccgc tttctatggt tttgtctaag
    22321 gcttccgggt tcatatagcc cagcattaac acttcgccag ataccgcgtg ttgcacaatt
    22381 accggcataa gtccgtcggt tttttcccag tccagttcgt gacgttgttg ttctgttaac
    22441 atatcctgat ctccacgccc tgtgttgcca ggtacgcttt taattcacca atattgatta
    22501 tttgtttgtg gaatacggaa gctgccagcg cgccgtcaac gtcggcatcg cggaaggctt
    22561 cgaggaagtg ttccatggtg cccgcaccac cggaggcaat cagcgggacg tggcaaactt
    22621 cacgcacttt tttcagttgt tcgaggtcgt aaccgttacg tacgccgtcc tgattcatca
    22681 tgttgaggac gatttctccg gcacctcgtt tttgtacttc ctgtacccag tcgagcgttt
    22741 cccactgagt gacgcgggtg cggctttcat cgccggtata ttgattcaca tgatatttac
    22801 cggtttcagc gtt
//
