LOCUS       GU220362               15464 bp    DNA     linear   BCT 29-JUN-2010
DEFINITION  Escherichia coli serogroup O61 O-antigen gene cluster, complete
            sequence.
ACCESSION   GU220362
VERSION     GU220362.1  GI:299482794
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15464)
  AUTHORS   Li,X., Perepelov,A.V., Wang,Q., Senchenkova,S.N., Liu,B.,
            Shevelev,S.D., Guo,X., Shashkov,A.S., Chen,W., Wang,L. and
            Knirel,Y.A.
  TITLE     Structural and genetic characterization of the O-antigen of
            Escherichia coli O161 containing a derivative of a higher acidic
            diamino sugar, legionaminic acid
  JOURNAL   Carbohydr. Res. 345 (11), 1581-1587 (2010)
   PUBMED   20510395
REFERENCE   2  (bases 1 to 15464)
  AUTHORS   Li,X., Perepelov,A.V., Wang,Q., Senchenkova,S.N., Liu,B.,
            Shevelev,S.D., Guo,X., Shashkov,A.S., Feng,L., Wang,L. and
            Knirel,Y.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P. R. China
FEATURES             Location/Qualifiers
     source          1..15464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O61"
     gene            626..1816
                     /gene="elg1"
     CDS             626..1816
                     /gene="elg1"
                     /note="nucleoside-diphosphate sugar epimerases"
                     /codon_start=1
                     /transl_table=11
                     /product="Elg1"
                     /protein_id="ADJ19205.1"
                     /db_xref="GI:299482795"
                     /translation="MGNILQLIGRNKALFGDDVSENEEELQRIVATSRFLVLGGAGSI
                     GQAVTKEIFKRNPQKLHVVDISENNMVELVRDIRSSFGYIDGDFQTFALDIGSVEYDA
                     FIKADGKYDYVLNLSALKHVRSEKDPFTLMRMIEVNILNTEKTIQQSIAAGVKKFFCV
                     STDKAANPVNMMGASKRIMEMFLMRKSEEIAISTARFANVAFSDGSLLHGFNQRLQKQ
                     QPIVAPHDIKRYFVTPQESGELCLMSCIFGENRDIFFPKLNEALHLISFADIAVLYLK
                     QRGYEPHLCETEDEARVLAKTLPAQGKWPCLFTSSDTTGEKDFEEFFTDKEVLDMKRF
                     INLGIIKNDPLYDPMLLDHFKEKIEHMRASLEWSKKDIVKLFFEMIPDFGHKETGKYL
                     DSKM"
     misc_feature    626..13965
                     /note="O-antigen gene cluster"
     gene            1831..2973
                     /gene="elg2"
     CDS             1831..2973
                     /gene="elg2"
                     /note="aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="Elg2"
                     /protein_id="ADJ19206.1"
                     /db_xref="GI:299482796"
                     /translation="MNINSIIEFVRDVYKTNEFIPLHAPVFDGNEKKYVLDTLESTFV
                     SSVGKYVDDFGRKMEAYTGTARAVATVNGTAALSAALYLAGVKRGDLVVTQALTFVAT
                     CNALYHLGAEPVFIDVSPVSLGLCPVALDSWLSENTELTEHGCQHRTTHQIVRAVVPM
                     HTFGHPVEMDELIAVCKKWRIVLVEDAAESLGSFYKGLHTGTLGEYGALSFNGNKIIT
                     TGGGGMVFCRASEEGVRAKHVTTTAKVPHPYEFYHDEPGFNYRMPNLNAALGCGQMER
                     LDMFLKQKRTLAQRYQTFFEGSEFKFVKEPQYAQSNYWLNAVICENLDSRDAILAQMN
                     EAKVMTRPIWKLMHRLPMFEHAMRDDLKNSEQIEARLVNLPSSPVE"
     gene            2979..4142
                     /gene="elg3"
     CDS             2979..4142
                     /gene="elg3"
                     /note="UDP-N-acetylglucosamine"
                     /codon_start=1
                     /transl_table=11
                     /product="Elg3"
                     /protein_id="ADJ19207.1"
                     /db_xref="GI:299482797"
                     /translation="MTTTTRKVAVFTGTRAEYGLLYWLMKDIQSDEELQLQLLVSGMH
                     LSPEFGSTWQQIEQDGFSIDEKIEILLSSDSPVGIAKSMGLGVLGFADALSRLKPDVL
                     VILGDRFEALAAAQTAMILRIPVFHLHGGEITEGAYDDAIRHAITKLSYLHGTSTEEY
                     KNRVVQLGENPARVTNVGAIGLEHLKRSKFMTVEELSTSLNFSLKKPYVVVTYHPVTL
                     GDEPAEASFTALLDALDKFPELQVILTYPNADDGGRKIIPLLEAYAAKSPERVKAIPS
                     LGQMRYLSAVKYASAVVGNSSSGIIEVPALDVPTVNIGVRQKGRIAAQSVLNCDATTE
                     SITAALTSAISRSYIREGEKISNPYGQGNASAKIIEMIKSMNFVPSKTFYDIK"
     gene            4149..5222
                     /gene="elg4"
     CDS             4149..5222
                     /gene="elg4"
                     /note="N-acetyl-neuraminic acid synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="Elg4"
                     /protein_id="ADJ19208.1"
                     /db_xref="GI:299482798"
                     /translation="MTLIIAEAGVNHNGDEKLAFKLVDAAHKAGADIVKFQTFKAKNL
                     VTAEAVQADYQVANTKKQESQLEMLSRLELSWEAHHKLVSYCNKLGIEFLSTAFDSES
                     LDFLVNELGVKRLKLPSGELTNAPLVLEHARTGCDIIVSTGMATLAEIEAALGVIAFG
                     YTAPEEAVPSIEAFQRAYSSEVGQKALKEKVIVLHCTTEYPAPVEEINLRAMDTLRQA
                     FGLPVGYSDHSEGIMIPVAAVARGAVVIEKHFTLDKNMEGPDHKASLEPVELEAMIAA
                     IHQIEKALGNSIKAPTVSEIKNKSVARKSLVAAKTIIAGESFTSDNLAIKRPGTGMSP
                     YLYWNLINEVSENDYLPGELNGE"
     gene            5212..5862
                     /gene="elg5"
     CDS             5212..5862
                     /gene="elg5"
                     /note="acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="Elg5"
                     /protein_id="ADJ19209.1"
                     /db_xref="GI:299482799"
                     /translation="MVNDNLQRKPLVIIGGGGHASVIVDILKRQKREIVAIISPDDIT
                     QRKVYSGIDVFSNDNEIFRFQPKDIRLINGIGALPDSEVRYKVNLYFEKMGYCFETIV
                     ADNAYVSPFAFLEEGVQIFPGAIIQPGTHIGAHTIINTRVVIEHDVSLGAYNAISPGA
                     IICGQCKTEERVFIGAGAIVIQNIEIGSRATIMANALVAENIHPQQKVYASRGIVR"
     gene            5881..6939
                     /gene="elg6"
     CDS             5881..6939
                     /gene="elg6"
                     /note="sugar-phosphate nucleotide transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="Elg6"
                     /protein_id="ADJ19210.1"
                     /db_xref="GI:299482800"
                     /translation="MNQQWKNVLISPDSSILEALEIINNEALRVALVVNENNTLLGVI
                     TDGDIRRGILKNLPLTAEVHQVMNKKPVTASPVLSKKELNNLMSSHGILSIPIVDKGI
                     IVGLETITSIAATEKYDNPVFIMAGGFGTRLRPLTDNCPKPMLKVGDKPILETVVRSF
                     VKAGFSNLYISTHFLPDMIHQHFGDGDAFNAKITYIHEETPLGTGGALGLLPDSLSDS
                     LPLIMINGDVLTNIDFERLLSFHNNNNADATICVRKYDYQIPYGVITGNGNKIVSMVE
                     KPVHHFFVNAGIYVVSPDIFKSVPKNHRIDMPTLLEQFMSKNKEILMFPIHEYWLDIG
                     RIDDFNRAQADIHSLGLD"
     gene            6942..7928
                     /gene="weiH"
     CDS             6942..7928
                     /gene="weiH"
                     /note="oxidoreductase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiH"
                     /protein_id="ADJ19211.1"
                     /db_xref="GI:299482801"
                     /translation="MKKVAVIGLGNIATRHRHNLKKLFPGIIVFSMSSSERVLSELVS
                     DCDGYLANVDAIIQEQVDFVIVASPATYHLRHSEKLLAAGIPYFLLKNPVTASFDDAK
                     KLHEIAERHATPVAIGYCLRYLPSAKIIKKIIEDKFIGSIYNVNIEIGQYLPDWRPSK
                     SYRESVSASKVLGGGALLELSHELDYAQWLFGELKLVNSVLRTSSELEMDVESLADII
                     VINSAGSLINIHLDFLQKKPWRQCHIIGSKGRIVWDLIRNEIIHHTRQSTDIIFSDPG
                     WDKNGMYTDMLLDFIAEISGSDNNCVTLESSINIVSLIDKIKETSQCLGEVQ"
     gene            7907..8620
                     /gene="elg7"
     CDS             7907..8620
                     /gene="elg7"
                     /note="cytidylyl-transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="Elg7"
                     /protein_id="ADJ19212.1"
                     /db_xref="GI:299482802"
                     /translation="MPWRSSMKINAFIFARGGSKGLPGKNIKPLAGKPLLQYSIETAK
                     QSPSISSIYVSTDDDDIALVAENCGATVIRRPAELAGDTSPEWLAWRHAIEWVQKEVG
                     DFDGFVSLPTTSPLRSVDDVECAIAKRVESGADICISVTPASRSPYFNMVKFHESGYV
                     RLVNEPEGKVLRRQDSPDVFDITTVVYATTPKFVLNNYGLFSGKVASIIVPKERAVDI
                     DDIFDFYMAEILLKELNRG"
     gene            8610..9374
                     /gene="weiI"
     CDS             8610..9374
                     /gene="weiI"
                     /note="oxidoreductase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiI"
                     /protein_id="ADJ19213.1"
                     /db_xref="GI:299482803"
                     /translation="MVANILKGKKILIAGAGGLLGTHLVKKVIDEGGYVIAGDFDLVS
                     TQNKLNELGITTGYELHQLDVTSLESVQEILAIAPDLDGAINTTYPRNKTYGAHFYDV
                     TLESFNENLSLHLGSSFLFSQQCAAYFKKNQRSFSLVNISSIYGVVAPKFEIYENTKM
                     TMPVEYAAIKSALLHLNKYIVAYVRDSRFRVNAVSPGGIFDHQPDAFLEAYKKETNGA
                     GMLGVTEMLGSIVFLLSDASKYVTGQNIIVDDGFSL"
     gene            9371..9925
                     /gene="weiJ"
     CDS             9371..9925
                     /gene="weiJ"
                     /note="galactoside O-acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiJ"
                     /protein_id="ADJ19214.1"
                     /db_xref="GI:299482804"
                     /translation="MISFIKKIKFWIVCNRLGPDIPLSHLLLYSRRLGRMICKRKFKS
                     FGNNSSFRPFAYAIETQKIAIGDNVVIRPGTMLFASPYGEEKKLHILIEDDVLIGSSV
                     HIYVSNHKFFDITLPISKQGHSVVKPVILKKGCWIGANVTILPGVVVGENSVVGANSV
                     VTKSISPFTVVAGNPAKIIKKLNE"
     gene            9918..11144
                     /gene="wzx"
     CDS             9918..11144
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADJ19215.1"
                     /db_xref="GI:299482805"
                     /translation="MSEYKRIFQYLAFDLTNKVLPFVVPYFIASYLSSVELGYYTTKQ
                     LLFLLFFNIISMGGGAKLLVSISKKDGEEKKISSILLKMFCFNACVILFFYVLLPFFI
                     VSENVIKDYIPLVICSLFYSIIQLQLSIYRGYNRINAYGMLNLSLSVCVCIVIFSYIL
                     YFKTQLGLWYWLIIPYALFSIKFLKYYLTERVLSSAVLLDTLKFCFYQFPHVLSSWCR
                     LGIDRLFLANIFAMSLVGYYSMMLQFGLIVSAVLQSLNNYYSPYLFRVLSERQSYKKL
                     SLFSKNNKAARSSFLFFVASFVIVIVVNIFAYVVVHYFLPSEYSPYYYLVPLVTFAYG
                     LQGCYFAVVNYIYFWGKTQYLNIPSILSCLFQVVIGYFFILHFSLLGASLSLLMSWSL
                     QLLFTLGGVMYVAKNK"
     gene            11125..12078
                     /gene="weiK"
     CDS             11125..12078
                     /gene="weiK"
                     /note="hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiK"
                     /protein_id="ADJ19216.1"
                     /db_xref="GI:299482806"
                     /translation="MLQKTNKKGFQLALVESLLQLKTLDSYSGNNKNNIHLFVRLNGE
                     QKNEEEILNFIKPRACHYSSVQFVSIRRNDKFSLLFNILKLRLFLFCKRKVILIIGDP
                     RALWMNMISSFKNVHDVIYLEDGMSTVLFYQTFKPKYPHKHYKLVTRLKLDGNAFLSL
                     IPLEVKKNTVMRIDNDVALFIGMPMIENNALSKKKYLSYLHKIIMSLKNMKITKFYYA
                     PHRYENENNFYLYENLGFHMLDTDCAIEDYLNSKNIIPAVYASFYSTALLQIDTLFYG
                     VSVICYVINVEELNYDFRNPALYAYEYYNKTPSIIKVDLHD"
     gene            12071..13228
                     /gene="wzy"
     CDS             12071..13228
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADJ19217.1"
                     /db_xref="GI:299482807"
                     /translation="MIEFLLFILCSFILYQVFYVTKEFKSNLFLIMWGYTFLFVTPVI
                     YIFYGGEKYRVFSDESALTFYLLGCLSAAFIILMLLFKVSLNRIKICKINLFISDFIL
                     KIIFSFCIMFVVLYILFYWREWPFFDFVSGDISDRPDIVKGTFQGFFIYSLFTSIIIP
                     GIYFHLKDKKGKLFNLLFFIFVCFSMVVSGNKGVFLYFIIFNVLFEWKKIRLSTYLII
                     IVGLMAIYALIRLPFIGDNFSLSYLIESISERIFLTQGMAMPAVIELAKSTDVTMMNS
                     NDLKYTLFNFVYGYSPGSMPLFYTAELYVRYGWLMMSFISVIISLVFGFGAFVINKTK
                     DSAIRWVYYISLYALIMGGVGSANLFFFIVAILWWLLLTLSNGTITSRSGK"
     gene            13249..13965
                     /gene="weiL"
     CDS             13249..13965
                     /gene="wfgD"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgD"
                     /protein_id="ADJ19218.1"
                     /db_xref="GI:299482808"
                     /translation="MPMYNAEHYVRASICSILNQTFKNFLLYIIDDCSTDSSKQIAES
                     FNDPRIIIISNAINVGVARTRNKGIELAQTKYIAFCDSDDIWHEQKLEKQISLLDSGK
                     YNVVGSFYSTFKDGKFESAKLISAPELVCYRDMLKSNWIGNLTGIYNAYVLGKVFQQE
                     IGHEDYVMWLKLIEKSRVAYIIQEPLAYYRIRSSSLSSNKMKACLWQWRIYRKMLHFN
                     IFRTSCYMFFYIIAALNKRR"
BASE COUNT     4482 a   2660 c   3382 g   4940 t
ORIGIN
        1 cgtattcagc tgactgatgc tattgccgaa ctggcgaaaa aacagtccgt tgacgccatg
       61 ctgatgaccg gcgacagcta cgactgcggt aaaaaaatgg gctatatgca ggcgttcgtg
      121 aagtatggac tacgcaacct caaagaaggg gcgaagttcc gtaaaggtat tgagaagctg
      181 ttaagcgaat aatgaaaatc tgaccgaatg taacggttga taagaaaatt ataacggcgg
      241 tgaagattcg tggcgaaagt aatttgttgc gaatattcct gccgttattt tatataaaca
      301 atcagaataa caacgagtta gcaataggat tttagtcaaa gttttccagg attttccttg
      361 tttccatagc tgattggtaa gacaattagt gtttgaattt ttcaggttta gcgcgagtgt
      421 gtaacgctcg tcacatcgta ggcatgtacg cagtgctctg gtagctataa agccaggggc
      481 ggtagcgtgt gcaaatctga gttaaggcag cgattgtggg ggctataggt atgtatagct
      541 gctcgttctg tagatatgct tgaaccagag cagcttctga ctcaggcatg tcgtcgcaat
      601 aatatttaat caacgggatc gtaaaatggg taatattctc caacttattg gacgcaataa
      661 agcattattc ggtgatgatg tatctgaaaa tgaagaagaa ttacaaagaa ttgtcgctac
      721 atcccgtttt ttggttctgg gcggtgcagg ctctatcggg caggctgtta caaaagagat
      781 atttaaacgt aatcctcaaa agctccatgt tgtcgatatt agtgaaaata atatggttga
      841 gctggtacga gatatacgta gctctttcgg ttatattgat ggggatttcc aaacctttgc
      901 tctggatatt ggttcggttg agtatgacgc atttatcaaa gctgatggaa aatacgatta
      961 tgtcctgaat ttgtcagcgc ttaagcatgt tcgcagtgaa aaagatccgt ttactttaat
     1021 gcgtatgatt gaagtaaaca tccttaatac agagaaaact attcaacaat ctattgcagc
     1081 cggtgttaag aaatttttct gtgtttctac cgataaagcc gcaaaccctg tgaatatgat
     1141 gggggcttcc aagcgtatta tggaaatgtt cctgatgcgc aagagcgaag agatcgctat
     1201 ttctactgct cgttttgcaa atgtagcatt ttcagatggc tcactgcttc atggttttaa
     1261 tcagcgactg cagaaacaac agccaattgt ggctcctcac gatatcaagc gctattttgt
     1321 gactccacag gaatctggtg agttgtgttt gatgtcctgt atcttcggcg agaatcgtga
     1381 catctttttc cccaaattaa atgaggcact gcatcttatc tcctttgccg atatcgcagt
     1441 tttgtatttg aaacagcgtg gctacgaacc tcatctctgt gagacggaag atgaggctcg
     1501 tgtgttggcg aaaactttgc cagcgcaggg aaagtggcca tgccttttca catccagcga
     1561 caccactggt gaaaaagatt tcgaggaatt ttttaccgac aaagaggtgc ttgatatgaa
     1621 gcgctttatt aatctcggca ttatcaagaa tgatccactt tacgatccca tgcttttaga
     1681 ccattttaaa gaaaaaattg agcacatgag ggcgtcgctg gagtggagta aaaaagacat
     1741 tgtaaaactg ttttttgaaa tgattccaga ttttgggcat aaagaaacag gtaaatatct
     1801 tgacagtaaa atgtaagtag gcctcctcgt atgaatatca attcaattat cgaatttgtc
     1861 cgagacgtat acaaaacgaa tgagtttatt cctttgcatg cgccagtttt tgatggcaat
     1921 gaaaaaaaat atgtattaga tacacttgaa agcaccttcg tttcaagcgt aggcaaatat
     1981 gtcgatgact ttgggcgcaa gatggaggct tatactggaa cggcgagagc tgttgcgaca
     2041 gtaaatggta ctgcggcatt aagtgctgcg ctgtatctgg ctggggtgaa gcgcggcgac
     2101 ttagtggtta cccaagcgct gacttttgtt gcaacctgta atgcccttta tcatttgggg
     2161 gctgaacctg tatttataga tgtctcgcct gtcagtctcg gtctttgccc tgttgcgctg
     2221 gatagctggc tttctgaaaa taccgagctg actgagcatg ggtgccagca tcgtacaaca
     2281 catcagattg ttcgcgctgt ggtgccaatg cacacttttg gtcatcctgt tgaaatggat
     2341 gaacttattg cggtctgcaa gaagtggcga attgttctgg tagaagatgc ggcagaaagc
     2401 ttgggttcct tttataaggg gctgcatact ggtacgcttg gtgagtatgg ggctctgagt
     2461 tttaatggaa ataagattat cactactggt ggtggtggta tggttttttg tcgcgcgtct
     2521 gaggaaggtg tgcgagcgaa gcatgtcacc acaaccgcca aggttccaca tccatatgag
     2581 ttttaccacg atgagcctgg ttttaactac cgtatgccca acctgaatgc tgctctggga
     2641 tgtggacaaa tggaacgatt ggatatgttt ttaaagcaaa aacgcacgct tgcccagcgt
     2701 tatcaaacat tcttcgaagg gtctgagttt aaattcgtta aagaacctca atatgctcag
     2761 tctaattact ggcttaatgc cgtaatctgt gaaaacttgg attcacgtga cgctattttg
     2821 gcacaaatga atgaagcaaa agtgatgaca cgcccgatat ggaaactgat gcaccgctta
     2881 ccaatgtttg agcacgcaat gcgggatgat cttaaaaact ctgagcaaat tgaggctcgc
     2941 ttggtcaact tgcctagctc tcctgtggaa taagtgcgat gacaacaaca acacgcaagg
     3001 tcgcagtttt taccggaact cgagcagaat atggattact ctattggtta atgaaggaca
     3061 tccagagcga cgaggagctg caactgcaac tgctcgtcag tggtatgcac ctttcccctg
     3121 aatttggcag tacatggcaa cagatcgagc aggatggttt ttcaattgat gagaaaattg
     3181 aaattttgct ctcctctgat tctccggtcg gtatagcaaa aagcatgggg ttgggggttc
     3241 tggggtttgc tgatgcatta tcccggctga aacctgatgt tttggtcatt cttggcgatc
     3301 gttttgaagc tcttgcggcg gcgcaaaccg cgatgattct tcgtattcct gttttccatc
     3361 tacatggcgg tgaaattact gaaggtgcct acgatgatgc tattaggcac gccatcacga
     3421 aattgagtta tttacacggt acttccacgg aagaatataa aaaccgtgtc gttcagttgg
     3481 gtgaaaatcc ggctcgtgtt actaatgtgg gtgctattgg gttagagcat ctgaagcgta
     3541 gcaagtttat gacggtcgaa gagttatcaa catcattgaa tttttcgcta aaaaagccgt
     3601 atgtagttgt cacatatcat ccggttactt taggtgatga gcctgctgag gcgagcttta
     3661 cagcattgct ggacgcgctt gataaatttc ccgagcttca ggttatcctg acctacccga
     3721 atgcggatga tggcggcaga aaaattattc ctttacttga agcttatgct gctaaatcac
     3781 ctgaacgcgt caaagctatc ccgtcacttg gacagatgcg ttatctaagt gcggttaaat
     3841 atgcttcagc ggtggtggga aactcttcca gcggcataat tgaggtgcct gctctcgacg
     3901 ttcctacggt aaatattggc gtgcgtcaaa aagggcgtat tgccgcgcaa agcgtactca
     3961 attgtgatgc tacaacggaa tctatcactg ctgcactaac gagtgcgatt tcgcgaagtt
     4021 acataaggga aggagaaaaa atatccaatc cttatgggca agggaatgcg agtgctaaga
     4081 tcattgagat gatcaaatcc atgaattttg taccgagcaa gacattctac gacattaagt
     4141 gaaattgtat gacgcttatt attgctgaag ccggtgttaa ccacaacggc gacgaaaaac
     4201 tggcttttaa acttgttgat gctgctcata aagcaggtgc tgatatcgtc aaatttcaga
     4261 cgttcaaggc gaaaaacttg gtaacagctg aagcagtaca ggcggactat caggttgcga
     4321 acactaagaa gcaagaatca cagttagaaa tgctaagtcg gttggagctt tcatgggaag
     4381 ctcaccacaa gctggttagt tattgtaata aacttggtat cgaattcctt tcaaccgcct
     4441 ttgattccga gagcttggat tttcttgtta atgagcttgg cgtcaaaagg ctaaagttac
     4501 cttcaggtga attgactaac gcgcctttgg tacttgagca tgcgcgcact ggttgcgaca
     4561 ttattgtctc aacgggaatg gcgacattgg ctgagattga ggccgcgttg ggtgttattg
     4621 ctttcggata tacagcgcct gaagaggcag tgccgagtat tgaagctttt cagcgtgcgt
     4681 actcgtctga ggttggtcaa aaagcgctta aggaaaaggt tatcgttctt cattgcacta
     4741 cggaatatcc tgcgccagtg gaagagatta atctacgtgc tatggatacg ttgcgtcagg
     4801 cgtttggatt acctgtgggg tattccgatc acagtgaagg gataatgatt cctgtggccg
     4861 ctgttgcacg tggtgcggtt gtgatcgaaa aacacttcac acttgataaa aacatggaag
     4921 gaccggacca caaagcatcg ctggagcccg ttgaactgga ggcaatgatt gccgctattc
     4981 atcagataga aaaagcgcta gggaatagca ttaaagcgcc tactgtgtca gaaatcaaaa
     5041 acaagagtgt tgcgcgaaaa agcctagttg ctgcaaaaac tattatagcg ggggaaagct
     5101 tcacatccga taatctggca ataaagcgcc ctggtacagg tatgtctcct tatttatatt
     5161 ggaatttaat aaatgaagtt tctgaaaatg attatttacc gggggagttg aatggtgaat
     5221 gataatcttc agagaaaacc acttgttatt attggtgggg gagggcacgc tagcgtaatt
     5281 gttgatattt tgaaaagaca gaagcgagag attgttgcta ttattagccc tgatgatatc
     5341 actcagcgaa aggtatattc aggaattgat gttttttcga atgataatga aatctttcgt
     5401 ttccaaccaa aggatattcg cttaattaat ggaattggtg ccttaccaga ctcggaagtt
     5461 aggtataaag ttaatttgta ctttgaaaaa atgggctatt gttttgaaac aattgtagct
     5521 gataacgcat atgtgtcacc ctttgccttt ttggaagaag gagtacagat atttcctggt
     5581 gccataattc aaccagggac acatattggt gcccatacta ttattaatac ccgtgtagtc
     5641 attgagcatg atgtatctct cggtgcatat aacgcaatct ctcctggggc tataatatgt
     5701 gggcagtgta aaactgaaga gcgtgtattc attggtgcag gtgcaattgt tattcagaat
     5761 attgaaatcg gatcaagagc cacaatcatg gcgaacgcac tggtagcaga aaatatacat
     5821 ccccaacaaa aagtttatgc ttcacgcggt atagtcagat aatgtttcag aaggtatata
     5881 atgaatcagc aatggaaaaa tgtattaatc tcaccagata gctcgatact tgaagcactc
     5941 gaaattatca ataacgaagc attaagagtt gcgctggtag ttaatgagaa taacacgtta
     6001 ttaggagtca ttaccgatgg tgatattcga agaggaattt tgaaaaacct gcctctgact
     6061 gcagaagtac accaggttat gaataaaaaa cccgtaacgg caagtccagt tctttccaaa
     6121 aaagaactca ataatctgat gtcatcgcat gggatactgt ctataccaat agtcgataag
     6181 ggtatcattg tggggctgga gactattaca agcatcgctg caacggaaaa atatgataat
     6241 cctgttttta ttatggccgg agggtttggt actcgtttga gaccactcac tgataattgc
     6301 cctaaaccaa tgcttaaagt aggtgataaa cctattttag agacagtggt aagaagcttt
     6361 gttaaagcag ggtttagtaa tttatatata tcaacccatt ttctgccaga tatgattcat
     6421 cagcacttcg gtgatggtga tgcgtttaat gcaaaaatca cctatataca tgaagaaact
     6481 cccttaggta cgggaggagc gctgggattg cttcctgact ccttatcaga ttctttgcca
     6541 ctcattatga tcaatggtga tgtgcttacc aatattgatt ttgagcggtt actatcattt
     6601 cataataata acaatgcgga tgctacaatc tgcgtaagaa aatatgacta ccagatccca
     6661 tatggtgtta taacaggtaa tggtaataaa atagtcagta tggttgaaaa gccagtacat
     6721 catttctttg ttaatgcagg aatctatgtt gtttctcccg atatttttaa atcagtaccc
     6781 aaaaaccatc gaattgatat gccgacgtta ctcgaacaat ttatgagtaa gaataaagag
     6841 atactaatgt tcccgattca tgagtattgg cttgatatag ggcgaataga tgattttaat
     6901 cgggcacagg ccgatattca ttctttaggg ctggattaaa aatgaaaaag gtcgctgtaa
     6961 tcgggttggg aaatatcgca acaagacatc gccacaatct taaaaaactt tttccaggaa
     7021 ttattgtttt ttctatgtct tcaagtgagc gagtgctatc cgagttagtg agtgactgtg
     7081 atggttactt agctaatgta gatgctatca tacaagagca agtcgatttt gtaattgtag
     7141 catcaccagc aacttaccat ttacggcata gtgaaaaact tttggcagca ggaattccct
     7201 actttttatt gaaaaaccct gttacagctt cgtttgatga cgctaagaag ttacatgaaa
     7261 tagctgaaag acacgcaact ccggtggcta taggatattg tttacgctat ttgccttctg
     7321 caaaaataat taaaaaaata attgaggata aatttattgg cagtatttac aatgtcaata
     7381 ttgagattgg tcaatatctg ccggattgga gaccgtcaaa atcatatcgt gaaagtgtat
     7441 cagcaagtaa agttttgggt ggcggagcgc tactcgaatt aagtcatgag ctggattatg
     7501 cgcaatggct atttggtgaa ctgaaacttg tgaattcagt gctacggact tcgtcagaac
     7561 ttgaaatgga tgtcgaatct ttagctgata ttatagtgat aaattccgcc ggttcgctta
     7621 ttaatatcca tcttgatttc cttcaaaaga aaccatggcg acaatgtcat attattggca
     7681 gtaagggacg aattgtctgg gatcttatcc gcaatgaaat cattcatcat actcgacaga
     7741 gtaccgatat tatctttagt gatcctggct gggataaaaa tggtatgtat acagatatgc
     7801 tgctcgactt cattgcggaa atatccggga gtgataacaa ctgtgtgaca ttggagtcat
     7861 caataaatat tgttagctta attgataaga taaaagaaac aagtcaatgc cttggagaag
     7921 ttcaatgaaa ataaacgctt ttatatttgc acgtggggga tcaaaaggat tacctggtaa
     7981 aaatattaaa cctctagcag gcaaacctct cctgcaatac tctattgaga ctgccaaaca
     8041 gagtccttcg atttcctcta tatatgtttc aaccgacgat gatgatattg ctctcgttgc
     8101 agaaaattgt ggtgctactg ttattcgtag accggcggag cttgccggag atactagtcc
     8161 agaatggttg gcatggcgtc acgcaattga gtgggttcag aaggaagtgg gggatttcga
     8221 tggctttgta agtttaccga ctacaagccc tttgcgaagt gtcgatgatg tcgaatgtgc
     8281 tattgctaaa agagtcgagt ctggtgctga catatgcatt tcagtcactc cggcgagcag
     8341 aagtccttat tttaatatgg taaaatttca cgaaagtggt tatgtgaggc ttgtcaatga
     8401 accggaaggg aaagtgctca gacggcagga ttcaccagac gtatttgata ttactactgt
     8461 tgtgtacgcg acaacgccaa aatttgtttt gaataattat gggctattct caggcaaagt
     8521 tgcaagcatt attgttccta aagaacgtgc cgttgatata gatgatattt ttgattttta
     8581 catggcggaa attttactta aggaattgaa tcgtggctaa tatacttaaa gggaaaaaaa
     8641 tccttattgc tggtgccggt ggattgctag gcactcatct tgttaaaaaa gtcatagatg
     8701 aaggtggtta tgttattgcc ggtgattttg acttagtgtc aacacagaac aaattaaatg
     8761 agctgggtat cacaactgga tatgaattac atcaacttga tgtaacaagt cttgaatctg
     8821 ttcaggaaat actagccatt gcacctgatc tggatggtgc tattaatacg acttatcctc
     8881 gaaataaaac ttatggtgcc cacttttacg atgtaacgct ggaaagtttc aacgaaaatc
     8941 tttcgttgca tcttggaagt tcatttcttt tctcacaaca gtgtgcagca tatttcaaaa
     9001 aaaatcagcg ttcattttca cttgtaaata tttcttctat ttatggtgtc gtggcaccaa
     9061 aattcgaaat atacgaaaat actaaaatga cgatgcctgt tgaatatgcg gcaataaaat
     9121 cggcattgtt acatttaaac aaatatattg tagcttatgt tagagatagt cgatttagag
     9181 ttaatgctgt aagccctggc ggtatttttg atcatcaacc agatgctttt ttagaagcat
     9241 ataaaaaaga aactaatggt gcagggatgc ttggtgttac agaaatgctt ggaagtattg
     9301 tatttttgct ttctgatgcg tcaaaatatg taacgggaca aaatattatt gttgatgacg
     9361 gcttttcatt atgatttctt ttataaaaaa aataaaattt tggattgtgt gcaacaggtt
     9421 aggacctgat ataccactta gtcacttatt attatattca cgtcgcttgg ggcgtatgat
     9481 ttgtaaacgg aaattcaaaa gttttggcaa taactcttct tttcggcctt ttgcatatgc
     9541 tattgaaaca caaaagattg ctatcggtga taatgttgtt atcaggcctg gtacaatgtt
     9601 atttgcttca ccatatggtg aagaaaaaaa acttcatatc cttattgaag atgatgtttt
     9661 aattggttct tctgtacata tttatgtatc caaccataag ttttttgata taacattacc
     9721 tatctctaag caaggtcatt cagtggtgaa acctgttatt ctgaaaaaag gttgttggat
     9781 tggtgctaat gtaaccattt tacctggagt ggtagtcggt gaaaattcgg ttgttggtgc
     9841 aaatagtgta gttacgaaaa gtatctctcc ttttacagtt gttgcaggaa acccagccaa
     9901 aattataaag aaattaaatg agtgaatata aaagaatttt tcagtattta gcatttgacc
     9961 ttacaaataa ggttttacca ttcgtagttc catactttat tgcttcctat ttgagttcag
    10021 tagagttagg gtattataca actaaacagc ttttattttt actgttcttt aatatcataa
    10081 gtatgggggg aggcgcaaaa ttactagtct ctatctcaaa gaaagatgga gaggagaaaa
    10141 aaatctcatc cattttatta aaaatgtttt gttttaatgc ctgtgttatt ttattttttt
    10201 atgtattact tccttttttc attgttagtg aaaatgtaat taaagattat ataccattgg
    10261 taatatgctc tttattttat tctattatac agctgcaatt gtcgatttat agaggatata
    10321 atagaataaa tgcttacggg atgttaaatc taagcctctc agtttgtgtt tgtatcgtga
    10381 tattcagtta tatattatat ttcaaaacgc aactagggtt atggtattgg ctaattatac
    10441 catatgcact tttttctatt aaatttttga aatattatct gacagagcga gtattatcat
    10501 ctgctgtttt acttgataca ttaaaatttt gtttttatca atttcctcat gtgctaagtt
    10561 cgtggtgtcg tttaggtatt gataggctat ttttggctaa tatattcgct atgtcattgg
    10621 ttggatacta ctcaatgatg cttcagtttg gtttgattgt tagcgcagta cttcagtcat
    10681 tgaataatta ttattcccct tatctcttca gagttctctc agaaagacaa tcgtataaaa
    10741 aactgtctct atttagtaaa aataataaag ctgctcgttc atcgtttctg ttttttgtag
    10801 cctcttttgt tatcgttata gtggtgaata tttttgcata tgtcgttgtt cattattttt
    10861 tacccagcga gtattctcca tattactatc tggtaccatt ggttactttt gcctatggat
    10921 tgcaaggatg ctactttgca gttgttaatt atatttattt ttgggggaag actcagtatc
    10981 ttaatatccc atcgatatta tcatgcttat ttcaggtggt aattggctac ttctttatct
    11041 tacatttctc tctattggga gcatcattaa gtttattaat gtcttggagt ttgcaactgc
    11101 tatttacatt agggggggtg atgtatgttg caaaaaacaa ataaaaaggg atttcaattg
    11161 gctttagttg agtccttact gcaactaaaa actttagata gttattctgg aaataataaa
    11221 aataacattc atctttttgt tcgactaaat ggtgagcaga aaaatgagga ggagatactc
    11281 aattttatta aaccaagagc atgtcattat tcttcagttc aatttgtaag cattcgacga
    11341 aatgataagt tctctttact ctttaatatt ctgaaattaa gattatttct tttttgtaaa
    11401 cgaaaggtaa ttttgatcat tggtgatcca cgcgctctgt ggatgaatat gatatcatcg
    11461 tttaaaaatg ttcatgatgt aatatactta gaggatggaa tgtcaacagt cctcttttat
    11521 cagaccttta aacctaagta cccacataaa cattataaac tggttacacg cctgaaactg
    11581 gatggtaatg cctttctgtc tcttattcct ttggaagtaa aaaagaatac ggttatgcgg
    11641 atcgacaatg atgtggcttt gttcatcggt atgccgatga ttgaaaataa cgcgttgagt
    11701 aaaaagaaat atttatctta tctgcataaa attattatgt ctttgaagaa tatgaagata
    11761 acaaagtttt attatgctcc ccatagatat gaaaatgaaa ataattttta tttatatgag
    11821 aatttgggtt ttcatatgtt agatacagat tgtgctattg aagattatct caacagcaag
    11881 aacattatcc cggcggtgta tgctagtttt tattcaacag ccttattaca gatagatact
    11941 ttattttatg gagttagtgt tatttgttat gtaattaatg ttgaagagtt gaattatgac
    12001 tttcgtaatc cagcattata tgcatatgaa tattataaca aaactccctc tattataaag
    12061 gttgatttgc atgattgaat ttttattgtt catattatgt agctttatct tatatcaggt
    12121 tttttatgtt actaaggaat ttaagagtaa tcttttctta atcatgtggg ggtatacttt
    12181 tctattcgta actcccgtta tatacatttt ttatggtggt gagaaatatc gtgtctttag
    12241 tgatgaaagt gcattaacat tttatttgtt gggttgtctc tccgctgcgt ttataatttt
    12301 aatgcttctg ttcaaggttt cacttaaccg aataaaaata tgtaaaatca atttatttat
    12361 ctccgatttt atattaaaaa ttatattttc tttctgtata atgtttgttg ttctttatat
    12421 tctattttat tggagggagt ggcctttttt tgattttgtc tcgggagata tctcagaccg
    12481 tccggatatt gtaaaaggaa cctttcaagg tttttttatc tattctttat ttacgagcat
    12541 aataatcccc gggatatatt ttcatttaaa agataaaaaa ggaaaattgt ttaatttact
    12601 tttctttatt tttgtttgtt ttagtatggt ggtaagtggt aataaaggtg tttttctata
    12661 ttttattatt tttaatgtgt tatttgaatg gaaaaagata cgtctaagta cgtatttgat
    12721 tattatcgta ggtctgatgg ctatctatgc cttaattcgt ctgccattta taggtgacaa
    12781 tttttcttta tcatatctaa ttgaatcgat atctgagagg atttttttaa cgcaagggat
    12841 ggctatgcca gccgttatcg agttagcaaa gtcaaccgat gtgacaatga tgaactctaa
    12901 tgatctaaaa tacacacttt ttaatttcgt ttatggctat agccccggtt cgatgcctct
    12961 tttttataca gctgaattat atgtcaggta tggttggtta atgatgtctt tcatatctgt
    13021 aataatttcg cttgtatttg gatttggtgc atttgttatt aataagacaa aggattctgc
    13081 cattagatgg gtttattata tctctttata tgcactaata atgggagggg taggaagtgc
    13141 taatcttttc ttttttattg tggcaatttt atggtggtta ttattaactc tcagtaatgg
    13201 aacaattaca agtaggagtg gcaagtgatt gcagaagtat caataattat gccgatgtac
    13261 aatgctgaac attatgtcag agcgtcaatt tgttcaattt taaatcagac gtttaaaaat
    13321 tttttattat atattattga cgactgctct acggattcat caaagcagat tgctgaatct
    13381 tttaacgacc cgcgaataat cattatttcg aatgctatca atgttggagt tgctcgaact
    13441 cgtaataaag gaatagagct tgcgcagacg aaatatatcg cattttgtga tagtgatgat
    13501 atctggcatg agcaaaaact ggaaaaacaa atctcactac ttgatagtgg gaaatataat
    13561 gttgttggaa gtttttactc aacttttaag gatggaaagt ttgaaagtgc aaagttaata
    13621 tctgcgcctg aacttgtttg ttatcgtgat atgcttaaat ctaattggat tggtaatctt
    13681 actggcattt ataatgctta tgttttaggt aaggtttttc aacaagaaat tggtcatgaa
    13741 gattatgtaa tgtggttgaa acttattgaa aaaagtcgag ttgcttacat tattcaagaa
    13801 cctttggctt attataggat taggtcctca tctctttctt cgaataaaat gaaagcttgc
    13861 ttgtggcagt ggagaattta tcggaaaatg cttcatttta atatttttag aacttcttgt
    13921 tatatgttct tttatattat tgctgcatta aataaaagac ggtaattatt taatgcacat
    13981 attttataag tgagtaaatt ttacaattac tgggttaatt taaggggtgg aatatttctg
    14041 ttattgttta tgtttttaat atagaatcga tgtaaattaa aacctcacag gtcgtgtgga
    14101 gaccgcatct gacaggagta aacaatgtca aagcaacaga tcggcgtcgt cggtatggca
    14161 gagatggggc gcaaccttgc gctcaacatc gaaagccgtg gttataccgt ctctattttc
    14221 aaccgttccc gtgaaaagac ggaagaagtg attgccgaaa atccaggcaa gaaactggtt
    14281 ccttactata cggtgaaaga gtttgttgaa tctctggaaa cgcctcgtcg catcctgtta
    14341 atggtgaaag caggtgcagg cacggatgct gctattgatt ccctcaagcc atacctcgat
    14401 aaaggtgaca tcatcattga tggtggtaac accttcttcc aggacaccat tcgtcgtaac
    14461 cgtgagcttt ctgcagaagg ctttaacttt atcggtaccg gtgtttccgg tggtgaagaa
    14521 ggcgcgctga aaggtccttc cattatgcct ggtgggcaga aagaagccta tgaactggtt
    14581 gcgccgatcc tgaccaaaat cgccgcagtg gctgaagacg gtgagccatg cgttacctat
    14641 attggtgccg atggtgcagg tcactatgtg aagatggttc acaacggtat tgaatacggt
    14701 gatatgcaac tgattgctga agcctattct ctgcttaaag gtggtctgaa cctctccaac
    14761 gaagaactgg cgcagacctt taccgagtgg aataacggtg aactgagcag ctacctgatt
    14821 gacatcacta aagacatctt cactaaaaaa gatgaagacg gtaactacct ggttgatgtg
    14881 attctggatg aagcagctaa caaaggtacc ggtaaatgga ccagccagag cgcgctggat
    14941 ctcggtgaac cgctgtcgct gattaccgag tctgtgtttg cacgttatat ctcttctctg
    15001 aaagatcagc gtgttgccgc atctaaagtt ctctctggcc cgcaagcaca gccagcaggc
    15061 gacaaggctg agttcatcga aaaagttcgc cgtgcgctgt atctgggcaa aatcgtttct
    15121 tacgctcagg gcttctctca gctacgcgcc gcgtctgaag agtacaactg ggatctgaac
    15181 tacggtgaaa tcgcgaagat tttccgtgct ggctgcatca tccgtgcgca gttcctgcag
    15241 aaaatcaccg atgcatatgc cgaaaatccg cagatcgcta acctgatgct ggctccgtac
    15301 ttcaagcaaa tcgccgatga ctaccagcag gcgctgcgcg atgtcgtcgc ttacgcggta
    15361 cagaacggta tcccggttcc gaccttcgcc gctgcggttg cctattatga cagctaccgc
    15421 gccgctgttc tgcctgcgaa cctgatccag gcccagcgcg acta
//
