LOCUS       AB812026               22182 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: K11a.
ACCESSION   AB812026
VERSION     AB812026.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 22182)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..22182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="K11a"
                     /db_xref="taxon:562"
                     /note="serogroup:O65"
     gene            <1..914
                     /gene="wcaM"
     CDS             <1..914
                     /gene="wcaM"
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01126.1"
                     /translation="HANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDI
                     LISDHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIH
                     VENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYG
                     VIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIAMKRASLELH
                     NKPQHLFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQ
                     SSVDIDRINHHIVNVEKINFRLPERRE"
     gene            1077..1982
                     /gene="galF"
     CDS             1077..1982
                     /gene="galF"
                     /note="O065_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01127.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            2355..3431
                     /gene="rmlB"
     CDS             2355..3431
                     /gene="rmlB"
                     /note="O065_02"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAQ01128.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADVCDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
     gene            3428..4291
                     /gene="rmlA"
     CDS             3428..4291
                     /gene="rmlA"
                     /note="O065_03"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAQ01129.1"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIQDILIITTPEDQQGFIRLLGDGSQFGVNLTYAIQPSPDGLAQAFIIGENFIGNDTV
                     CLVLGDNIFFGQGFTPKLRDAAERTSGATVFGYQVMDPERFGVVEFDRNFKALSIEEK
                     PANPKSNWAVTGLYFYDNNVIDIAKGIKPSKRGELEITAVNEVYLRNNNLTVELLGRG
                     FAWLDTGTHDSLIEAGSFVETVQKRQGMMVACLEEIAWRNGWLSKIKLYELGHKLSKN
                     QYGQYLIKLAS"
     CDS             4288..4689
                     /note="O065_04"
                     /gene="qdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="putative dTDP-6-deoxy-3,4-keto-hexulose
                     isomerase"
                     /protein_id="BAQ01130.1"
                     /translation="MTMKIELLPLQIHGDSRGSLISLEYAINIPFDIKRVYYIFNTKE
                     GVTRGYHAHKQLKQIAIAITGSCDFTMDNGKERITLTLNNPAQGLLIDSCIWREMSNF
                     TPDCVLMILASNEYDESDYIRDYEEFKLMVK"
     CDS             4690..5142
                     /note="O065_05"
                     /gene="qdtB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="BAQ01131.1"
                     /translation="MFIHELSDVQTEHIGENTRVWQYSVILPEAIIGEDCNICAHTLI
                     ENKVTIGNRVTIKSGVYIWDGVTIEDDVFIGPGVVFTNDKYPRSKRYPEQYPTIIIKK
                     GASIGANATILPGVTVGEGAMVGAGSVVTKNVEPNTIVIGNPAKELKR"
     CDS             5144..6256
                     /note="O065_06"
                     /gene="qdtC"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminotransferase"
                     /protein_id="BAQ01132.1"
                     /translation="MMIEFLNLKKINNQYKDELKEACAKVIDSGWYVLGEYLEEFEKD
                     YSKWCGVKHTIGVANGLDALSLVLRAWKEMGMLKDGDEIIVPANTYIASILAITANRL
                     VPVLTEPDLITYNLSPKNIEKNITKKTRAILPVHLYGNISPMDDIMALASKYKLLVLE
                     DSAQAHGASINGRKAGAWGNASGFSFYPGKNLGALGDAGAVTTNDDELAYVVKALRNY
                     GSHTKYKNLYEGVNSRLDEIQAAMLSVKLKYIDKEVEKRRIIARSYLKNIKNILIKLP
                     TVLDDERHAWHLFVVRTCQRERFQAYLSTNGIQTLIHYPTPPHLQDAYKNKRVTSGFS
                     LPITEMIHNEVVSLPMDPTMSDKDIEHVISTVNRFK"
     gene            6253..7503
                     /gene="wzx"
     CDS             6253..7503
                     /gene="wzx"
                     /note="O065_07"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01133.1"
                     /translation="MNLIRTSLLSVISTFFRLLSALVINKAIATFIGPTGLALIGQFQ
                     NFTQIALVLAQGGINNGVVKYNAELISENKDITILNSTAIRISLLSSLFIGFLMAIFS
                     NYLSDYIFNSPNYSYVFIVFGITIFFFVINQLLLSILNGLKEIRLFIGINISQSIYSL
                     IFTSLAILTYGLVGALIAMVTNQSIIFITVVWRLRKHPIISLRLFNANYDKKIANKLF
                     KYSVMALTSILTVPLTNFILRNYIGNVIGWSQAGYWQAIWYISSMYLMVITTALNIYY
                     LPRLSEIKDKKEIKKELLNGYVLIIPFIVLLTLGIFFLKEYIVLILFNKDFYPMLELF
                     KWQLVGDVIKISAWLLSYIMLAKAMTKEFIITEILFSMLFVIVSLIYINVFGLIGVTY
                     GYATNYIIYFIVMFLIFRKKYLLQ"
     CDS             7511..8419
                     /note="O065_08"
                     /gene="wbqE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01134.1"
                     /translation="MRLTNNSYPLVSVAIITYNQKEYLRECIESILLQNYPNLQIVVA
                     DDCSSDGTQDLLLRYSQQYPNVFTLKLSEKNLGITANSNLAHFACRGKYIFWMGGDDL
                     MLPGKIAKQVEYMENNPDCTLCYHNLDVFDSATKKNLYYFNHLKKQKINGTIREIIKF
                     RTFNGACSTVVRKDKTPKEGFNELIPIASDWLYWVDTLANGGNIKYIDEVLGKYRRHD
                     NNITKKTKCLSQADIDHLNTCNIIIGKYPQYLSEALYAYAEILFHLRKKLDYKKIVCF
                     SLGANFRIRKAIALGVFLLSFGIIKV"
     CDS             8422..10311
                     /note="O065_09"
                     /gene="wceT"
                     /codon_start=1
                     /transl_table=11
                     /product="putative asparagine synthase"
                     /protein_id="BAQ01135.1"
                     /translation="MCGIIGYIDFDKNSNADVLKKMTDSLHHRGPDDSGILDEEFNNC
                     KVGFGHRRLSILDLSSHGHQPMTFMNLSIVYNGEVYNFKEIREQLEKEHYVFNSDSDT
                     EVLLKGWHCWGEDLIQKLNGMFALAVLDRTNQTVTLLRDRAGVKPLYWYRKNNLILFS
                     SELKGLHRHNAFSKEISKEGVNLYFQYGYIPQPYTIFENVYKLESGHCLKIDLVNQAI
                     QKNCYWNVFDYYRKEKIKLTESEAIVKCESLLSSACEYRMIADVPVGVFLSGGYDSSI
                     VAALLQRNNSKKLKTFTIGFKEKSYNEAGYAKDISNYLGTEHTEYYCTSDDALNILPL
                     LPDICDEPFADPSIIPTILVSRLAKKDVTVSLSADGGDELFAGYDKYTGIKKKIDIFN
                     IIPNMAYPGIRKILETKLAHKAFEYAGYTDVNNRLFRLAQMIGQDEVRILKSASIIFS
                     DLELNHLLGGADEIYTGFDDRLNSNWLDNLLAIDYKTYQVDDILFKVDRATMSCSLEG
                     REPLLDHRLIEFVARLEPELKIKNGDRKYILKKIAHKYIPQSLLDRPKQGFGIPLSEW
                     LGGQLKNYVSYYLNPKRIAESGILNPVFVDSLCRDYYSGNINKVNFKKIWVLLMFEMW
                     REKWM"
     CDS             10290..11429
                     /note="O065_10"
                     /gene="wceU"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01136.1"
                     /translation="MERKMDVKNKKITIFISSLRPGGAEGVCVTVANGLVKLGWDVTI
                     VVLNMIRATHQQRLDTSINIVNLNCRHAREAIFPIWRFLSSSASSNLLVFNHQLAIIL
                     VLIRIFTRKNYIIISRNINTLSVDLKNAKNFWHKHIVSFLIKRFYSKVEHIIAQSHGM
                     KDDLINFLNINASKISVIHNPVNPYFSDFAKAIKWNDYDKDGFILCVGRLERQKAFHF
                     AVDIFSKVAEKRQDLNLFIIGEGSLRQELEDYVSRLKVTRKVKFLGLRNDLEKYYSKA
                     SVVLLTSLYEGFPNVLVESLTLGTPVVAFDCPNGPNEIINSNNGLLVNYKDVNDGVEK
                     LNKALSQEWDYVEIAKAASIYSEEYIVNIYSDVLKQTFYSTELLR"
     gene            11426..12697
                     /gene="wzy"
     CDS             11426..12697
                     /gene="wzy"
                     /note="O065_11"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01137.1"
                     /translation="MIYFTVLVMLFILGVLSLIKKSNLFSPSILFPLVIVTTLLPSLL
                     LPSVFYTPSVYSVLYIFVCTTSFSFFSFLFEQINIKSKLSCNVLSFNMSGYYFIAFVT
                     FILCFLMLDEFLNKIAGFANFGAYAEYYRAASTSGEDDLKDSVNYLVRNADMLALPLS
                     VIGLYIWSTRRVSSIFKITVVIFILFSLTLPIISVARSGAIRACIVLLGVFVIFFRFR
                     LKFAILVLSFVVALYVGAGFMFGYGSGGYSSFSESIGYFLVSFIRYLSGGYYTFDSYL
                     LGKVDIFFRFAFVDSILGKIDTTLTLAGMGGKCIVCYPENHAEFVQVGPAENFVSNVY
                     TVFGVVFSNLGWLGVFYFSLCGAIMGSLYKAMKSGRVFFAIVYSFLLPGVVLASFSEY
                     FFAQIPLLIRFILLYMILFKLSAHKNKLEGF"
     CDS             12699..13823
                     /note="O065_12"
                     /gene="wdaB"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01138.1"
                     /translation="MKKKVLYVVSTLKRSGPINQLYNLINNFSKYEFDLTIVTLSPEP
                     QDSKLDDFINLGINVITLGLSRLKGVFFAKHKLKKIIDKINPDIVHSQGVRADYLNYL
                     IVGGHMKISTIHNYPQKDFIKRYGPIVGTVLVKIQINAIKNFDIVIGVSKAVAKNLIS
                     TYKIKNAYAVINGIDTDIYKPKSSTDKSKLKEKLGLAKYDCIWISTGHLSVLKQPEIL
                     IKSFLKNKNDARFINSVFIFLGDGELSESLIMKYKHEKKIIFAGRVHNVVEYLQASDY
                     FASASSTEGLPMAVIEALSCGLPVLLSRIGPHEEIIGLDESIGVLFDSDDASESFMTM
                     VTKDKTALSHSCRSLALNFFSAEAMGANYKDIYNKSIKTK"
     CDS             13820..14629
                     /note="O065_13"
                     /gene="wfbZ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01139.1"
                     /translation="MKFSVLMSVYKDDNPLYLNQSIESIWIHQTLKPTQIVLVQDGVL
                     TEALYGVVNKWKQKVGDSFTIVSLDRNMGLGAALNRGLLYCKYELVARMDSDDIALPD
                     RFQIQVEYMIKNCEIIASSGYVEEWDEEMGTLLGIRYVPLETDSIIKMARFRSPLSHP
                     ASIFRRDIVLSLGGYPELRKAQDFALWSLLLKNKYKLSNLGTVLVKMRAGNSMIYRRG
                     FSHLRYEMELLKYQRMIGFLPFGLFCVNFVLKAGLRLSPGFVKKIAYKFLR"
     CDS             14660..15676
                     /note="O065_14"
                     /gene="gne"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-glucose 4-epimerase"
                     /protein_id="BAQ01140.1"
                     /translation="MSILITGGAGYIGSHTVLQLLRNEYDVVVIDNLINSNLESLKRV
                     EGLVGKKITTYIGDVTDSTFLKTIFDNHSVTDVIHFAGLKSVSESINDPLLYYKNNVQ
                     GSLCLITEMAKAGVYNLIFSSSATVYGSPQSVPLTEMSPLGLTTNPYGTSKLMIEQIL
                     RDFTNSNPNFKVVCLRYFNPVGADPSGCIGESPIGTPNNIFPYLTQVALGKFPYLSIF
                     GDDYPTIDGTGVRDYIHVVDLALGHLAALKFKQNETSFAVFNLGTGTGYSVLELVNTF
                     EEVTGIKIPCRIVGRRPGDVAECWSDPSLAKKVLFWEAKKTLVDMVRDAWNWQQKNPS
                     GYSS"
     gene            15775..17181
                     /gene="gnd"
     CDS             15775..17181
                     /gene="gnd"
                     /note="O065_15"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01141.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLNKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGDYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            17430..18596
                     /gene="ugd"
     CDS             17430..18596
                     /gene="ugd"
                     /note="O065_16"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01142.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSERAERFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVLVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLTDVADKVYTRDLFGND"
     CDS             complement(18662..19666)
                     /note="O065_17"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide sugar epimerase"
                     /protein_id="BAQ01143.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPQWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            20018..21052
                     /gene="wzz"
     CDS             20018..21052
                     /gene="wzz"
                     /note="O065_18"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01144.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLVVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGS
                     AAPKVSEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAQYIQQVDDQVNEELEQDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(21238..21849)
                     /gene="hisI"
     CDS             complement(21238..21849)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01145.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLHQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     6764 a   3830 c   4735 g   6853 t
ORIGIN
        1 cccacgctaa ttacgccatc ttacgccagg gatttcataa ccagattatc ggtgccaaca
       61 tcaccaactg taagttcagc gacttacagg gcgacgcaat tgaatggaac gtggcgatta
      121 acgacagtga tattctcatc tctgaccatg tcatcgagcg catcaactgt actaatggca
      181 aaatcaactg gggcatcggc ataggtcttg cgggaagtac ctacgataac aactacccgg
      241 aagaccaggc agtgaaaaac tttgtcgtgg cgaatatcac gggatcggat tgtcggcagt
      301 tgatacatgt tgaaaatggt aaacattttg ttattcgtaa tatcaaagcc cgcaatatca
      361 cgccggattt cagtaagaaa gcaggtattg ataacgccac agtcgctatt tacggttgtg
      421 acaatttcgt gattgataat attgaaatga ttaatagcgc cgggatgtta atcggctatg
      481 gggtaattaa aggcaaatat ctctcgatac cgcaaaattt ccgagtgaat aatattcagc
      541 tggataacac ccatcttgct tataaattgc gcggcatcca aatctccgcc gggaatgctg
      601 tttcctttgt ggcactaact aacattgcga tgaagcgtgc gtcattggag ctgcacaaca
      661 agccgcaaca cctttttatg cgcaatatca atgtgatgca ggaatcctca gttggacccg
      721 cattgagcat gaactttgac atgcgcaaag acgttcgagg cgtttttatg gcgaaaaaag
      781 aaacactgct ttctcttgca aatgttcatg cggtgaatga aaaaggacaa agctccgtcg
      841 atatcgacag gattaatcac catattgtta atgtggaaaa gattaacttt agattgccgg
      901 aacggagaga gtagatttgc gaccattcct ggaaaaatgg agccatactt aggaacaatg
      961 ctactgcaat ccacaacgaa gcggcgtaac atcacaagta attcagtaat caattcaggg
     1021 taattgatgc tggcgaaaaa aatcgaacaa gctataattc agcaaccatt ttacaggtgg
     1081 atgaaataat gacgaattta aaagcagtta ttcctgtagc gggtcttggg atgcatatgt
     1141 tgcctgccac taaggcgatt cccaaagaga tgctaccgat cgtcgacaag ccaatgattc
     1201 agtacattgt tgacgagatt gtggctgcag ggatcaaaga aatcctcctg gtaactcacg
     1261 cgtccaagaa cgcggtcgaa aaccacttcg acacctctta tgaattagaa tctctccttg
     1321 aacagcgcgt aaagcgtcaa ctgctggcgg aagtacagtc catttgcccg ccgggcgtga
     1381 ctattatgaa cgtgcgtcag ggcgaacctt taggtttggg ccactccatt ttatgtgcac
     1441 gacctgccat tggtgacaac ccatttgtcg tggtgctgcc agacgttgtg atcgatgacg
     1501 ccagcgccga cccgctgcgc tacaaccttg cggccatgat tgcgcgcttc aacgaaacgg
     1561 gccgcagcca ggtgctggca aaacgtatgc caggtgacct ctctgaatac tccgtcattc
     1621 agaccaaaga gccgctggac cgcgaaggta aagtcagccg cattgttgaa tttatcgaaa
     1681 aaccggatca gccgcagacg ctggactcag acatcatggc cgttggtcgc tatgtgcttt
     1741 ctgccgatat ttggccggaa cttgaacgca ctcagccagg tgcatgggga cgtatccaac
     1801 tgacagatgc cattgccgaa ctggcgaaaa aacagtctgt tgatgcaatg ctgatgactg
     1861 gtgacagcta cgactgcggt aaaaaaatgg gctatatgca ggcgttcgtg aagtatgggc
     1921 tgcgcaacct caaagaaggg gcgaagttcc gtaaagggat tgagaagctg ttaagcgaat
     1981 aatgaaaatc tgaccggatg taacggttga taagaaaatt ataacggcag tgaagattcg
     2041 tggcgaaagt aatttgttgc gaatattcct gccgttgttt tatataaaca atcagaataa
     2101 caacgagtta gcaataggat tttagtcaaa gttttccagg attttccttg tttccagagc
     2161 ggattggtaa gacaattagc gtttgaattt ttcgggttta gcgcgagtgg gtaacgctcg
     2221 tcacatcgta gacatgcatg cagtgctctg gtagctgtaa agccaggggc ggtagcgtgc
     2281 attaatacct ctattaatca aacaaagagc cgcttatttc acagcatgct ctgaagtaat
     2341 atggaataaa ttaagtgaaa atacttgtta ctggtggcgc aggatttatt ggttctgctg
     2401 tagttcgtca cattataaat aatacgcagg atagtgttgt taatgtcgat aaattaacgt
     2461 acgccggaaa cctggaatca cttgctgatg tttctgattc tgaacgctat gtttttgaac
     2521 atgcggatgt ttgcgatgca gctgcaatgg cacggatttt tgctcagcat cagccggatg
     2581 cagtgatgca cctggctgct gaaagccatg tggatcgttc aattacaggc cctgcggcat
     2641 ttattgaaac caatattgtt ggtacttatg tccttttgga agccgctcgc aattactggt
     2701 ctgctcttga tagcgataag aaaaatagct tccgttttca tcatatttct actgacgaag
     2761 tatatggtga tttgcctcat cctgacgagg taaataatac agaagaatta cccttattta
     2821 ctgagacaac agcttacgcg ccaagcagcc cttattccgc atcaaaagca tctagcgatc
     2881 atttagtccg tgcgtggaaa cgtacctatg gtttaccgac cattgtgact aactgttcga
     2941 ataactacgg tccttatcac tttccggaaa aattgattcc actagtaatt cttaatgctc
     3001 tggaaggtaa ggcattacct atttatggta aaggggatca aattcgcgac tggctgtatg
     3061 ttgaagatca tgcacgtgcg ttatataccg tcgtaaccga aggtaaagcg ggtgaaactt
     3121 ataacattgg tggacacaac gaaaagaaaa acatcgatgt agtgctcact atttgtgatt
     3181 tgttggatga gattgtaccg aaagagaaat cttaccgcga gcaaattact tatgttgccg
     3241 atcgtccggg acacgatcgc cgttatgcca ttgatgctga gaagattggt cgcgaattgg
     3301 gatggaaacc acaggaaacg tttgagagcg ggattcgtaa aacaattaac tggtatttga
     3361 ataataatga ttggtgccgg cgagttcaag atggctctta tcaacgtgag cgtttggggg
     3421 taattagatg aaaggaatta ttttagctgg tggttctgga acaaggcttt atccaattac
     3481 aatgggagtt tcaaagcagc tattacccat ttatgataaa ccgatgattt attatccttt
     3541 gtcagtgctg atgttggcag gtatacaaga tattcttatt attacaacgc cagaagatca
     3601 gcaggggttc ataagattgc ttggggatgg aagccaattt ggtgttaatc tgacatatgc
     3661 gattcaacct agtccagatg gtttagccca agcatttatt attggtgaaa actttattgg
     3721 aaatgataca gtatgccttg tgcttggaga taacattttt tttggacaag gttttacacc
     3781 caaactccgg gatgcagcag aaagaacatc aggtgccaca gtctttggat atcaagtaat
     3841 ggatcctgag agatttggag tagtggagtt tgatagaaat tttaaagctt taagtattga
     3901 agaaaaacct gccaatccaa aatcaaattg ggctgtcaca ggattatatt tttatgacaa
     3961 taacgttatc gatattgcaa agggtattaa accttctaaa cgtggtgaat tagaaattac
     4021 ggcagttaat gaagtttatc tcagaaataa taatttaact gttgaattac tagggcgtgg
     4081 ttttgcatgg ctggatacag gaacccatga cagcttgatt gaagctggga gttttgtaga
     4141 aacagtccaa aaaagacaag ggatgatggt tgcctgtctg gaggagatag catggcgaaa
     4201 tggttggtta agtaagataa aattgtatga gttaggtcac aaattgagta aaaatcagta
     4261 tggtcaatat ctcattaaac ttgcgagttg acaatgaaaa ttgaattact acccttacaa
     4321 atccacggtg atagtcgtgg atcattgata tctttagagt atgccattaa tatccctttt
     4381 gatataaaaa gagtttacta tatttttaat acaaaagaag gtgttactag aggttatcat
     4441 gctcataaac agttaaagca aattgcaata gccataaccg gttcatgtga tttcacgatg
     4501 gataatggaa aggagagaat tacattaaca ttaaacaatc cagcgcaagg attattgatt
     4561 gattcatgta tttggcgaga aatgagtaac tttactccag attgtgtatt aatgattttg
     4621 gcgagtaatg aatatgatga gtctgattat attcgtgact atgaagaatt taaattaatg
     4681 gtaaaataaa tgtttataca tgagctaagt gatgttcaaa cagaacatat tggtgagaac
     4741 accagagtat ggcagtattc agtaatttta cccgaagcaa taattggaga agattgtaat
     4801 atatgcgcac acactttaat agaaaataaa gtaacaattg gtaatagagt tacaataaag
     4861 tctggcgtat atatttggga tggtgtaact atagaggatg atgtttttat tggtcctggt
     4921 gtggttttta caaatgacaa atatcctcga tcaaagagat accctgaaca atatccaact
     4981 ataattataa aaaagggagc ttcaataggg gcaaatgcaa ctattttacc cggtgttact
     5041 gtaggcgaag gtgcaatggt aggtgctggt tcagtagtta ccaaaaatgt cgagcctaat
     5101 acaatagtaa tcggaaatcc agctaaagaa ttaaagagat aatgtgatga ttgaattttt
     5161 aaatttaaaa aaaataaaca atcaatataa agatgaatta aaggaagctt gtgcaaaagt
     5221 tattgattct ggttggtatg ttttaggaga atatctggaa gagtttgaaa aagattattc
     5281 aaagtggtgt ggtgtaaaac atactatcgg agtggctaac ggattggatg cattgtctct
     5341 cgttttacga gcttggaaag aaatggggat gctgaaagat ggagatgaaa taatcgtacc
     5401 agctaatacc tatatagcat ctattctggc cataactgct aatagattag ttccagttct
     5461 aactgaacca gatctaatta catataacct aagccctaaa aatatcgaaa aaaatatcac
     5521 aaagaaaacc agagcaatat tgccagtgca tctatatggt aatatttctc caatggatga
     5581 tattatggct ttggcatcaa aatataaatt actggttctt gaagatagtg cacaggcaca
     5641 tggagcttca ataaatggtc gcaaagctgg tgcttgggga aacgcttcag gtttcagctt
     5701 ctatccaggt aaaaaccttg gcgcattggg tgatgccggg gccgttacta ctaatgatga
     5761 tgaattagcg tatgttgtaa aagctttgag aaattatggt tcacatacaa aatataaaaa
     5821 cttatatgag ggagtaaata gtagactaga tgagattcag gcagcaatgc tgtcagttaa
     5881 acttaaatat atcgacaaag aagtagaaaa acgcagaata atagctcgta gttatctaaa
     5941 aaatataaaa aatatattaa tcaaactgcc aacagtatta gatgacgagc gtcatgcgtg
     6001 gcatcttttt gttgttcgaa cttgtcaacg tgaacgtttc caggcttatt taagcacaaa
     6061 tggcatacag acattaattc attacccaac cccaccacac ctacaagacg cttacaaaaa
     6121 caaaagagta acaagtggtt tttctttacc aatcacagaa atgatacata atgaagtagt
     6181 ttctttgcct atggatccga ctatgtctga taaagatata gagcatgtaa ttagcacggt
     6241 taatagattt aaatgaattt aataaggact tctttattaa gcgtaatctc gacatttttt
     6301 agattactat ctgctttagt aataaataaa gctattgcca cttttattgg cccaacgggt
     6361 ttagctctga ttggacaatt tcaaaacttt actcagattg cgcttgtgtt agctcaaggg
     6421 ggaataaata acggggttgt taaatataat gctgaattaa tttcagaaaa taaagatata
     6481 actattttaa atagtacggc cattcgaatc agcttgttat catcgttgtt cataggattc
     6541 ttaatggcca tcttttcaaa ttatttatca gattatatat ttaatagccc aaattacagt
     6601 tatgtattca ttgtttttgg cataacgata tttttttttg ttattaatca gcttttgttg
     6661 tctatattaa atggtttaaa agaaataagg ttattcatag gtattaatat atcgcaaagt
     6721 atttatagtt taatatttac atctttagca atactaacat atggattagt tggtgcttta
     6781 attgccatgg taactaatca gtcaattata tttataactg tggtgtggcg attaagaaaa
     6841 catccaatta ttagcctgag attatttaat gctaattatg ataagaaaat agcgaataaa
     6901 ttgtttaaat actctgtaat ggcattgaca agtatattaa cagtgccatt aacaaatttt
     6961 attcttcgca attatattgg aaatgtaatc ggatggtcac aagctggtta ttggcaagcg
     7021 atatggtaca tttccagtat gtatttgatg gttattacaa ctgctctcaa tatatactat
     7081 ttacctcgat tatctgaaat taaagataag aaagaaataa aaaaggagtt gttaaatggt
     7141 tatgtcctga ttataccttt tatagtatta ttaacgttgg gtatcttttt tttaaaagaa
     7201 tacattgttc ttatactttt taataaagat ttttacccaa tgcttgagtt gtttaagtgg
     7261 caactagtgg gggatgtgat aaaaataagc gcttggcttt tgagttatat aatgctagct
     7321 aaagcaatga ctaaagaatt tatcattacc gaaatattgt tctcaatgtt atttgtgatt
     7381 gtttcattga tatatataaa tgtatttggg ttaattggtg taacttatgg atatgcaaca
     7441 aattatataa tatattttat tgtgatgttt ttaatcttta gaaaaaaata tcttttacaa
     7501 taataatgag gtgcgcttga caaataatag ctatcctttg gtgagtgttg caattatcac
     7561 atataaccaa aaagaatatc ttagagaatg tattgagtct atattacttc agaactatcc
     7621 aaatttgcag atagtagtag cagatgattg ttcatcagat gggactcaag atttattatt
     7681 gagatacagt caacaatacc ctaatgtatt cacattaaaa ttatctgaga aaaatttagg
     7741 aataacagca aattctaatt tagcgcattt tgcgtgcaga ggtaagtata ttttttggat
     7801 gggtggagat gatttgatgc tgcccggaaa aatagcaaaa caagtagagt atatggaaaa
     7861 taatccagac tgtacattat gctatcataa tctagatgtt tttgactctg caactaaaaa
     7921 aaatttatac tatttcaatc atttaaaaaa acaaaaaatc aatgggacaa ttagagaaat
     7981 tatcaagttt aggactttta atggagcgtg ttctactgtt gtaagaaaag acaaaacacc
     8041 aaaggaaggg tttaatgaac ttatccctat tgcttctgat tggttatatt gggttgatac
     8101 attagcaaat ggaggaaata taaaatatat agatgaagtg ttagggaaat accgtcggca
     8161 tgataataac ataacaaaga aaacaaaatg tttatcccaa gcagatattg atcatttgaa
     8221 tacttgtaac attatcattg gtaagtatcc tcaatatcta agtgaagcat tatatgctta
     8281 cgctgagata ttatttcact taaggaagaa gttggattat aaaaaaattg tttgttttag
     8341 tttaggtgca aatttcagaa ttagaaaagc aatagccctt ggtgtctttt tattaagctt
     8401 cggaattatt aaggtttaag tatgtgtgga attattggtt atatagattt cgacaaaaat
     8461 tcgaatgctg atgtactaaa aaaaatgaca gactccttgc accatagagg accagatgat
     8521 agtggaattt tagacgaaga atttaataac tgtaaggtgg ggttcggcca cagaagatta
     8581 tccatacttg atttatcatc acatggacat cagcccatga catttatgaa tctttctatt
     8641 gtttataatg gtgaggttta taatttcaaa gaaatacggg aacaattaga aaaagaacat
     8701 tatgtattca attcagattc ggatacagaa gtgttgctta aaggttggca ctgctggggg
     8761 gaggatctaa tacagaaact taatggaatg tttgctcttg ctgtacttga tcgtactaat
     8821 caaacggtta cattattgcg tgatagagca ggagtcaagc ctctctattg gtatcgtaaa
     8881 aataatttga tattattttc tagtgaatta aaaggattac acagacacaa tgcgttttct
     8941 aaagaaatat caaaagaggg agttaatcta tattttcagt atggatatat accccagcca
     9001 tatacaatat tcgaaaatgt ttataagtta gagtctggac attgtttgaa aattgatctt
     9061 gtaaatcaag caatacagaa aaattgttat tggaatgttt ttgattatta tagaaaggaa
     9121 aagatcaaac ttacagagag tgaagctatc gtaaaatgcg aaagtttatt aagctctgcc
     9181 tgcgaatatc gcatgatcgc tgatgttcct gtcggtgttt tcttaagtgg tgggtatgat
     9241 tcctctatag ttgctgcatt gctacagcgc aataattcta aaaaattaaa gacatttact
     9301 ataggcttta aagaaaagtc atataatgaa gctggttatg caaaagatat ttcaaattat
     9361 ctaggtaccg aacatactga atattattgt acgagtgatg atgctttaaa tatactccct
     9421 cttcttcctg atatttgtga tgaaccattt gcggatccat caattatccc tacaatactt
     9481 gttagtaggt tagcgaaaaa agatgttacg gtttcattaa gtgcggatgg tggggatgag
     9541 ctctttgctg ggtatgataa atatactggt attaagaaaa aaattgatat ctttaatata
     9601 atacctaata tggcttatcc aggaattaga aaaattcttg agactaaatt agcacataag
     9661 gcgtttgaat atgccggtta tactgatgtc aataatcgcc tatttcgatt agcacaaatg
     9721 atagggcaag atgaagttag aatactaaaa tcagcatcta tcattttttc tgatttagag
     9781 cttaatcacc ttttaggcgg ggcagatgaa atctatactg gattcgatga tcgtttgaat
     9841 agcaactggc tcgataattt gctggcaatt gattacaaaa cctatcaggt tgatgatatt
     9901 ttatttaaag ttgatcgagc aactatgtct tgctctcttg aaggaagaga accgcttctt
     9961 gatcatagac taatagaatt tgttgcaagg ttagagcccg agttaaaaat aaaaaatggg
    10021 gacagaaaat atattttgaa gaaaatagcc cataaatata tacctcaatc attgttagat
    10081 cgtccaaaac aaggatttgg catcccactt tctgagtggc taggtgggca attaaaaaat
    10141 tatgtttctt attatcttaa tcccaaaaga atagctgagt ctggaatatt aaatcctgtt
    10201 tttgttgatt cattatgtcg agattattat tctggaaata taaataaagt taattttaaa
    10261 aaaatatggg ttttattaat gtttgaaatg tggagagaaa aatggatgta aagaacaaaa
    10321 aaataactat ttttatcagt tcgctacgtc cggggggggc cgagggagtt tgtgttacag
    10381 ttgctaatgg actcgtcaaa cttggttggg atgttacaat tgttgtactt aatatgataa
    10441 gagcgactca tcaacagagg ctcgatacat caattaatat cgttaatctg aattgcaggc
    10501 acgcacgcga agcaatattt cctatttgga gatttcttag ttcatcagcc tcttccaatt
    10561 tattagtgtt taaccatcaa ctagcaatta ttctggtatt gattagaatt tttactagga
    10621 aaaactatat aattatctct cgtaatatca acacattaag tgttgattta aaaaatgcaa
    10681 aaaatttttg gcacaaacat atcgttagtt tcttaataaa aagattttat tcaaaggttg
    10741 aacatataat agcacaatca catggaatga aagatgatct tattaatttc ttgaatatta
    10801 atgcaagtaa gatatctgta atccataatc ctgttaatcc ttatttttct gattttgcta
    10861 aagcaataaa atggaatgat tatgataagg atggatttat attatgtgtg ggcagactgg
    10921 aaagacaaaa agcttttcat tttgcggttg atattttctc gaaagttgct gagaaaagac
    10981 aagatttgaa tctgtttata ataggtgagg ggagtttgag acaagagcta gaagattatg
    11041 ttagcaggct taaagtcact agaaaagtca agtttcttgg actaagaaat gatcttgaga
    11101 aatattatag taaagcctct gtggtgttac ttacttcctt atatgaagga tttccaaatg
    11161 tattagtgga atcattaaca ttgggaacac cagttgtggc atttgattgc cctaatggac
    11221 cgaatgaaat tataaattct aataacggtt tgcttgttaa ttataaagat gtcaatgacg
    11281 gagtcgaaaa attaaataaa gcattgagtc aagaatggga ttatgtggaa attgcaaaag
    11341 ctgcaagtat atatagtgag gaatatattg ttaacattta ttccgatgtt ttaaaacaga
    11401 cattttactc aacagaactt ttaagatgat atactttaca gttttagtga tgttgttcat
    11461 attaggtgta ctcagtttaa ttaaaaaaag caatctgttt tcaccttcaa tattatttcc
    11521 tttagttatt gttacaacct tacttcccag cttacttctg ccaagtgttt tttatacacc
    11581 gtcagtatat tctgttttgt atatatttgt ttgtacaact tctttttctt ttttctcatt
    11641 tctatttgaa caaataaata ttaaatcaaa gttgtcttgc aatgtattat ctttcaatat
    11701 gtctgggtat tattttattg cttttgttac atttattttg tgttttttaa tgcttgatga
    11761 atttttaaac aaaattgcag ggtttgcaaa ctttggagca tatgccgaat attatagagc
    11821 tgcctcaaca tctggtgaag atgatctaaa ggatagtgta aattatttag taagaaatgc
    11881 tgatatgtta gcattaccat tatctgtaat tggattgtat atttggtcaa cgcgtagagt
    11941 atcaagcata tttaaaataa ccgttgtgat ctttatattg ttctctttaa cccttcctat
    12001 tattagcgtc gctcgttcag gtgctattag agcttgcatt gttctacttg gcgtgtttgt
    12061 tatatttttt cgatttaggt tgaaatttgc cattcttgta ttaagttttg ttgttgcgtt
    12121 atatgttggc gctggtttta tgtttggcta tggttctggt gggtattcat ctttttcaga
    12181 atctataggg tatttcttgg tatcctttat ccggtattta tctggtggtt attatacatt
    12241 tgattcatat ttgcttggca aggttgatat tttctttaga tttgcattcg tagatagcat
    12301 tcttggtaaa atcgatacaa cgttgacttt agctgggatg gggggtaagt gtattgtctg
    12361 ctacccagaa aatcatgctg agtttgtcca ggttggtcca gctgaaaact tcgtttcaaa
    12421 cgtctatact gtattcggtg ttgtatttag caacttaggt tggctaggag tattttactt
    12481 tagcttatgc ggtgcaatta tgggctcact ttacaaagct atgaaaagcg gtagagtctt
    12541 ttttgcaatt gtatatagtt tccttttacc tggagtagtc cttgcgtcat ttagtgaata
    12601 tttttttgct caaatcccgc tactaataag gttcattttg ttatatatga ttttatttaa
    12661 attgtctgct cataaaaata aactggaggg attttaaatt gaaaaaaaaa gttctttacg
    12721 ttgtgtcaac actaaaaagg tcagggccaa ttaatcaatt atacaatttg atcaataatt
    12781 tctcgaaata tgaatttgat ttgacgattg taactttatc gcccgaacca caagactcaa
    12841 aattagatga tttcattaac ttgggtatta atgttattac tcttgggctt agtagactca
    12901 agggggtatt ttttgccaaa cataaactaa aaaagataat tgataaaatt aatcctgaca
    12961 ttgtccattc tcagggggtt agagctgatt atttaaatta tttaattgtt ggcggccata
    13021 tgaaaattag cacaattcat aattatccgc aaaaagattt tattaaaaga tatggcccta
    13081 ttgttggtac cgttctggtt aagattcaaa taaatgccat aaagaatttc gatattgtta
    13141 taggtgtttc taaagctgtt gctaaaaatc taattagtac ttacaagatt aaaaatgcat
    13201 atgcagttat taatggtata gatactgata tttataaacc taaatccagc accgataaaa
    13261 gtaaactaaa agaaaaactg ggtttggcta aatatgattg catatggata tctactgggc
    13321 atcttagtgt attaaaacaa ccggagattc taattaaatc gtttttaaaa aataagaatg
    13381 atgctcgttt tatcaatagt gtttttattt ttttagggga tggagagctc agtgagtctc
    13441 ttattatgaa atacaaacat gaaaaaaaaa taatatttgc aggtcgtgtt cataatgtag
    13501 ttgagtattt gcaagcttcc gattatttcg catcagcatc tagtacagaa ggtttaccta
    13561 tggcagtaat tgaagcatta tcttgtgggt tacctgtctt attatcaaga attgggcctc
    13621 atgaagaaat aattgggctt gatgaaagca ttggagtgtt attcgatagt gatgacgcat
    13681 ctgaatcatt catgacaatg gttacaaagg ataaaacggc gctttctcac tcttgtcgaa
    13741 gtttggcgct taatttcttt agtgcagaag caatgggagc taactacaaa gatatatata
    13801 ataagagtat aaaaactaaa tgaaattttc agtattaatg tctgtttata aagatgataa
    13861 ccctttatat ttgaatcaat caatagaaag catctggata catcaaacat taaaaccaac
    13921 ccagatagtt ctggtccaag atggtgtatt aacagaggct ctctatggtg ttgtaaataa
    13981 atggaaacaa aaggtaggtg attcatttac cattgtttcg ctagatagaa atatggggct
    14041 gggggcagct ttaaatagag ggttattata ttgcaagtat gagttggttg ccaggatgga
    14101 tagtgatgat atcgcattac cagatagatt tcaaattcaa gttgaatata tgataaaaaa
    14161 ttgcgagatt attgcttcta gtggttatgt tgaggagtgg gatgaagaaa tgggtacatt
    14221 attgggtata cgatatgttc cattggaaac tgattcgatt ataaaaatgg cccgttttcg
    14281 ctcccctctt agtcatcctg catcaatatt tagacgtgat attgttttat ctttgggggg
    14341 gtatcctgaa ttacgtaagg cacaggattt tgccctttgg tctttattat taaagaataa
    14401 atacaagtta tcaaatttag ggactgtttt agtaaagatg cgagcaggaa atagtatgat
    14461 ttatagacgt ggattcagcc atttgagata tgaaatggaa cttcttaaat atcaacgaat
    14521 gatagggttt ttaccttttg gtttgttttg tgttaatttt gttttaaagg ctgggcttcg
    14581 tttatctccc ggatttgtaa agaagattgc atataaattt ttacgctaat agagcgcatg
    14641 tttaactcaa ggtgaatgta tgtccatcct aatcacaggt ggagccggtt atattggttc
    14701 tcacacagta ttacagttac tcaggaatga gtatgatgtt gttgttattg ataatttgat
    14761 taattctaat ctggagtctt taaagcgagt tgagggctta gttggaaaaa aaattacgac
    14821 ttatataggc gatgtaactg atagtacgtt tctaaaaacg atttttgata atcactctgt
    14881 caccgatgtt atacatttcg ctgggttaaa gtcagtttca gaatctatta atgatccctt
    14941 gctgtattat aaaaataatg tacaaggttc tctttgtctt attactgaaa tggcaaaggc
    15001 gggggtttat aatttaatat ttagttcctc agcaactgtg tatggttccc ctcagtcagt
    15061 accattgact gaaatgagtc cattaggact gacaacaaat ccatatggta cttctaaatt
    15121 aatgattgag cagattttaa gagatttcac taattccaat cctaatttta aagtcgtatg
    15181 cctaagatat tttaatcctg ttggtgctga tccttctggt tgtataggtg aatccccaat
    15241 aggaactcca aataatattt ttccttattt gactcaggtt gcgttgggaa aatttcccta
    15301 tttatcaatt tttggagatg attacccaac tatagacgga acaggcgttc gtgactatat
    15361 ccatgtggta gatttagcat tggggcatct tgcagcatta aagtttaaac aaaatgaaac
    15421 atcgtttgca gtttttaact taggaactgg gacaggctat tctgttcttg agttagttaa
    15481 tacttttgaa gaagtgacag gaattaagat cccatgtcgg atcgttggga gacggcctgg
    15541 agatgttgcg gaatgttggt cagatccttc tcttgccaaa aaagtacttt tttgggaggc
    15601 gaagaaaact ttggtcgata tggttcgtga tgcttggaat tggcaacaaa agaacccaag
    15661 tggatactca tcttaactca taaatgcaaa ctggattatt atttatattt cgtagagtaa
    15721 cttcaaataa atttttattt atgttctgag cagtcttcgt gacaggagta aacaatgtca
    15781 aagcaacaga tcggcgtcgt cggtatggca gtgatggggc gcaaccttgc gctcaacatc
    15841 gaaagccgtg gttataccgt ctctattttc aaccgttccc gtgagaaaac ggaagaagta
    15901 attgccgaaa acccaggcaa gaaactggtt ccttactata cggtgaaaga gtttgttgaa
    15961 tctctggaaa cgcctcgtcg catcctgtta atggtgaaag caggtgcagg cacggatgct
    16021 gctattgatt ccctcaagcc atacctcaat aaaggtgaca tcatcattga tggtggtaac
    16081 accttcttcc aggacaccat tcgtcgtaac cgtgagcttt ctgccgaagg ctttaacttc
    16141 attggtaccg gtgtctccgg tggtgaagaa ggcgcgctga aaggtccttc cattatgcct
    16201 ggtgggcaga aagaagccta tgaactggtt gcgccgatcc tgaccaaaat cgccgcagtg
    16261 gctgaagacg gtgagccatg cgttacctat attggtgccg atggcgcagg tcactatgtg
    16321 aagatggttc acaacggtat tgaatacgga gatatgcaac tgattgctga agcctattct
    16381 ctgcttaaag gtggcctgaa cctcaccaac gaagaactgg cgcagacctt taccgaatgg
    16441 aataacggtg aactgagcag ctacctgatc gacatcacca aagatatctt caccaaaaaa
    16501 gatgaagacg gtgactacct ggttgatgtg atcctggatg aagcggctaa caaaggtacg
    16561 ggcaaatgga ccagccagag cgcgctggat ctcggtgaac cactgtcgct tattaccgag
    16621 tctgtgtttg cacgttatat ctcgtctctg aaagatcagc gcgttgccgc gtctaaagtt
    16681 ctctctggcc cgcaagcgca gccagctggc gacaaggctg agttcatcga aaaagttcgt
    16741 cgtgctctgt atctgggcaa aatcgtttct tacgcccaag gcttctctca gctgcgtgct
    16801 gcgtctgaag agtacaactg ggatctgaac tacggcgaaa tcgcgaagat tttccgtgct
    16861 ggctgcatca tccgtgcgca gttcctgcag aaaatcaccg atgcatacgc cgaaaatccg
    16921 cagatcgcta acctgctgct ggctccgtac ttcaagcaaa ttgccgatga ctaccagcag
    16981 gcgctgcgtg atgtcgttgc ttatgcggta cagaacggta tcccggttcc gaccttcgcc
    17041 gctgcggttg cctattatga cagctaccgt gccgctgttc tgcctgcgaa cctgatccag
    17101 gcacagcgtg actattttgg tgcgcatact tataagcgca ttgataaaga aggtgtgttc
    17161 cataccgaat ggctggatta atctgattta aatcaattaa tcaatgcaag gcccggagaa
    17221 accctccggg cttttttatt atacaaagcg gcaggttatg gccttttttt ataatttata
    17281 gttaaaaacg cgatataata cagcgccgca cagcaggatc gctgccttga cagttcatct
    17341 acatcagcgt taaaaatccc gcagtagatg aagttgtggt ggtgaattaa tgaccactct
    17401 aaatgtttaa ccggaagaag tcagagctaa tgaaaataac aatttcagga acaggttatg
    17461 ttggtctttc aaatggtatt ctgattgcgc aaaaccacga agtggttgca ctggatatcg
    17521 ttcaggccaa agtggacatg cttaacaaga agcagtcacc gattgttgat aaggagattg
    17581 aagagtatct ggcaactaaa gatctcaatt tccgcgctac gacagataag tatgacgcgt
    17641 ataaaaatgc cgattacgtt attattgcca cacctaccga ttatgatccg aaaacgaatt
    17701 attttaatac ctcaagcgtg gaagcggtca ttcgcgatgt gacagaaatt aatcccaacg
    17761 cggtaatgat tataaaatca actatccctg ttggttttac agagtccatt aaagaacgtt
    17821 ttggtattga aaatgtgatc ttttcgcctg agtttttacg cgaagggaaa gcactttatg
    17881 ataacttaca cccatcacgc atagtgattg gagagcagtc tgaacgcgct gaacgttttg
    17941 cagcattatt acaagaaggt gctattaagc aagatattcc aacgttgttt actgactcaa
    18001 cggaagctga ggcgattaaa ctttttgcaa acacctatct ggcgatgcgc gtggcgtact
    18061 ttaacgagct ggatagttat gcggaaagtt taggtctgaa tactcgccag attatcgaag
    18121 gcgtttgtct cgatccgcgt attggcaacc attacaacaa tccgtcgttt ggttatggtg
    18181 gttattgtct gccgaaagat accaagcagt tactggcgaa ctaccagtct gtgccgaata
    18241 acctgatctc ggcaattgtc gatgccaatc gcacgcgaaa agattttatt gccgattcta
    18301 tccttgcacg taaaccgaaa gttgttggcg tctatcgtct gattatgaag agtggttcgg
    18361 acaatttccg tgcttcctcg attcagggta ttatgaagcg aatcaaggcg aaaggcgtgc
    18421 ttgtaatcgt ttatgagcca gcgatgaaag aggacgattt tttccggtcg cgcgtggtac
    18481 gtgatctgga tgcgttcaaa caagaagctg atgttattat ttctaaccgt atgtctgccg
    18541 atttgactga tgtagcagat aaggtttata cgcgcgactt gtttggcaat gattaattat
    18601 tttgtttcat actaagaaaa ggccctaata aattagggcc ttttcttatg gttttgtaaa
    18661 atcaaacttt atagaagtta cgataccatt ctacaaagtt ctttaccccc tctttaactg
    18721 acgtttcagg tttgaatcct attacgtcat acagtgcttt tgtatcagca ctggtttcca
    18781 gtacatcacc gggttggaga ggcatcatat ttttgttggc ttcaataccc agagcctctt
    18841 ctaacgcatt gatatagtcc atcaactcta caggcgaact attaccaatg ttatagacac
    18901 gatatggtgc tgaacttgtt gcaggcgagc ctgtttctac agcccactgt ggatcttttt
    18961 ctggaataac atcctgtaag cgaataatag cttcggcaat atcatcaata taagtaaagt
    19021 cacgcttcat tttgccgaag ttgtaaacat caatgctttt accttccagc atggctttag
    19081 taaatttaaa taatgccata tccggacgtc cccatggacc ataaaccgta aagaaacgca
    19141 gccctgtggt cggtaagcca tacaaatgag aatacgtatg ggccatgagt tcatttgctt
    19201 ttttagttgc tgcataaagc gaaacaggat gatctacaga gtcatctgta gagaaaggca
    19261 tcttgcggtt catgccataa acagaactgg aggaagcgta aagtagatgc tgaacattat
    19321 tatggcgaca tccttccagt atgttcagga atccaatcag gtttgcatct gcatatgcat
    19381 tgggattttc aagggagtaa cgtacaccgg cttgcgcagc gaggtttatt acgcgatcga
    19441 accgctcgtc tgcaaacagt gccgccattt tctcacgatc ggccaggtca attttataaa
    19501 aactgaaatt gtcgtgcttg agtaaatcaa gtcgtgcttg tttgaggttg acatcgtaat
    19561 aatcatttaa gttgtcaatc cctacaacct gatgaccagc tgcaagaagc cgtttactta
    19621 catagaaacc gataaagcca gcagctcccg taaccagaaa tttcatttat aatcctcgct
    19681 caggctagaa tatagccaat cttcatctgg cataactgaa agttaaatca taccgttaga
    19741 caagaaaaaa agataatcgg tatcagttct aaacctggct gttttttctg gtaacgtgct
    19801 cattttacaa tcaaagctgt tctaaactga ctatacaagc cgacgtcatt atttccaacc
    19861 gtatggcaga agagcttaag gatgtggcag ataaagtcta cacccgcgat ctctttggca
    19921 gtgactaaca tcctgttatc agggcgattt tcgccctgat tctcttatgt tctctaagta
    19981 ataattcatt gtttttatca tttatcctat agcattcatg gcgattatcg ctaaactatg
    20041 gcgacttaaa attatttcgt ccgttagggt aatgatgaga gtagaaaata ataatgtttc
    20101 tgggcaaaac catgatccgg aacagattga tttgattgat ttactggtcc aactatggcg
    20161 tggcaagatg actatcatca tttctgtcat tgtggctatt gccctggcta tagggtatct
    20221 ggtcgttgcc aaagaaaagt ggacttcaac agctatcgtt actcaacctg atgtgggaca
    20281 aatcgctggt tataacaatg ccataaatgt gatttatggt tcggcagcgc cgaaagtatc
    20341 tgagatccag tcgatgttaa ttggtcgttt cagcacgaca ttttctgcat tagcagaaac
    20401 gctggataat caggaagaac ctgaaaaact gaccattgag cctaccgtta aaaatcagtc
    20461 attaccttta gcggtgtctt atgtggggga aacacctgaa ggtgcgcaga agcagctggc
    20521 gcagtatatc cagcaagttg acgaccaggt aaatgaagaa ctggaacaag acctgaaaga
    20581 caacatcgca ttgcgaatga aaaacttgca ggattctctg aagacgcagg aagtggtcgc
    20641 tcaagaacag aaagatctgc gtatccgtca gattcaggaa gcgttgcagt atgcgaatca
    20701 ggcgcaggtg acaaagccgc agattcaaca gacacaggac gtaacccagg atactatgtt
    20761 ccttttgggg agcgaagcgc tggagtcgat gattaagcat gaagcgactc gtccgttggt
    20821 gttctcatca aactactatc agactcgtca aaacctgctg gatatcgaca atcttgacgt
    20881 tgataaactt gatattcatg cttaccgcta tgtaatgaaa ccgacgttac ctattcgtcg
    20941 cgatagcccg aaaaaggcaa ttaccttgat tctggcagtg ctgctgggcg gcatggttgg
    21001 cgcggggatt gtgcttgggc gtaacgcgct gcgtaattac aacgcgaaat aatattattg
    21061 tgcatttaag agaaacgggc agggtggtga caccatgccc gttttttttg ccggatgcga
    21121 tgctggcgca tcttatccgg cctacgtgtg ttgagataat gtgtaggcac gataagcttg
    21181 cgcatcgggc aatggctccg ggtgtgacaa caacatcaca cctgctcagc acgcaactca
    21241 ctgatgccgt ttacgcaggt tctcaattac cgtcgttaaa tccagatcct gatcctgcaa
    21301 caacaccagc aggtgataca tcaagtcaga tgcctcgttg gtcagctcaa agcggtcatg
    21361 taccgttgct gccagtgcgg tttccacgcc ttcttcgccc actttctgcg caatgcgttt
    21421 ggtgccgctg gcatacagtt tggcggtgta ggaggtttcc gggtcggcag atttacgttc
    21481 ggcgagcagt tgttccagtt gatgcaggaa cagccactgg tgagcggtgt cgccgaagca
    21541 gctgctggtg cctttgtggc aggtcgggcc gatgggattc gccagcacca gtaacgtgtc
    21601 gttgtcgcag tccggggcaa tactcactac gttgaggaaa ttgcccgacg tttcgccttt
    21661 ggtccacagt cgctgtttag tgcgcgagaa gaaggtgact ttaccgcttt cgatggtttt
    21721 gtctaaggct tccgggttca tgtagcccag cattaacact tcgccagata ccgcgtgttg
    21781 cacaatcacc ggcatcagtc cgtcggtttt ttcccagtct agttcgcgac gttgttgttc
    21841 tgttaacata tcctgatctc cacgccctgt gttgccaggt acgcttttaa ttcgccaata
    21901 ttgattattt gtttgtggaa tacggaagct gccagcgcac cgtcaacgtc ggcatcgcgg
    21961 aaggcttcca ggaagtgttc catggtgccc gcgccaccgg aggcaatcag cgggacgtgg
    22021 caaacttcac gcactttttt cagctgttcg aggtcgtaac cgttacgcac gccgtcctga
    22081 ttcatcatgt tgaggacgat ttctccggca ccgcgttttt gcacttcctg cacccagtcg
    22141 agtgtttccc actgagtgac gcgggtgcgg ctttcatcgc cg
//
