LOCUS       DQ069297               15137 bp    DNA     linear   BCT 05-APR-2007
DEFINITION  Escherichia coli serogroup O66 O antigen gene cluster, complete
            sequence.
ACCESSION   DQ069297
VERSION     DQ069297.1  GI:71044434
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15137)
  AUTHORS   Cheng,J., Liu,B., Bastin,D.A., Han,W., Wang,L. and Feng,L.
  TITLE     Genetic characterization of the Escherichia coli O66 antigen and
            functional identification of its wzy gene
  JOURNAL   J. Microbiol. 45 (1), 69-74 (2007)
   PUBMED   17342059
REFERENCE   2  (bases 1 to 15137)
  AUTHORS   Cheng,J., Liu,B., Bastin,D.A., Han,W., Wang,L. and Feng,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2005) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, Hong Da Street, TEDA, Tianjin
            300457, P.R.China
FEATURES             Location/Qualifiers
     source          1..15137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O66"
     gene            1137..2213
                     /gene="rmlB"
     CDS             1137..2213
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-D-glucose 4,6-dehydratase"
                     /protein_id="AAZ20755.1"
                     /db_xref="GI:71044435"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDTAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LNNSDWCRRVQDGSYQRERLGLVK"
     misc_feature    1137..14368
                     /note="O antigen gene cluster"
     gene            2210..3073
                     /gene="rmlA"
     CDS             2210..3073
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyl transferase"
                     /protein_id="AAZ20756.1"
                     /db_xref="GI:71044436"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLLLA
                     KIRDILIITTPEDHASFIRLLGDGSRFGINLSYAVQEKPEGLAQAFLIGSEFIGADKV
                     CLVLGDNIFFGQAFGKQLSKVIKELEGATVFGYKVRDPERFGVVEFDNDNKAVSIEEK
                     PVKAKSNWAVTGLYFYDNTVVDIAKNVKPSERGELEITSINETYLSQGKLKVEQLGRG
                     FAWLDTGTHDSLLDASHFVETVEKRQGYKIACLEEIAYNNNWLTKEDIRFLAKKLTKT
                     DYGKYLLELIK"
     gene            3070..3621
                     /gene="rmlC"
     CDS             3070..3621
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /protein_id="AAZ20757.1"
                     /db_xref="GI:71044437"
                     /translation="MNVIKTKIEDLVIIEPKIYNDERGYFYEFYNKNKFTELGINLDF
                     VQDNRSSSSQNVLRGLHFQKTKPQGKLVTVTKGEVFDVAVDLRKDSRTFGQYESVILT
                     EENKKIFYIPPGFAHGFCVLSNKADFLYKCTDFYDPNDEGGIIWNDSSLSIEWPVNAP
                     ILSKKDMELPTLKNIRQSLLDGF"
     gene            3611..4456
                     /gene="tll"
     CDS             3611..4456
                     /gene="tll"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-L-lyxo-hexulose reductase"
                     /protein_id="AAZ20758.1"
                     /db_xref="GI:71044438"
                     /translation="MDFKMSQFTIFGGSGFIGSEIVKKLKSEKKDVYVPRRDEDLCFG
                     KDLGIVIYCAGNGDCVNTPRSVYDANIRYLTDILFECNYKVLVYVSSTRVYINNDSSL
                     EGNDIIVTSCDERRLFNLTKLVSEELCNRSGKPVIIVRPSNVYGLAIKSPLFLPTITR
                     NAILNGSVDMFVEEEYEKDYVSVNDVVDCIIKLSMMDKAIGKTINIASGYNIAAKDLA
                     DILRNKTNCIINWHGNSGKRENFPIINIELLKSFIPEYKPRSVLADLEYMIKEFKHNI
                     DSKVL"
     gene            4453..5790
                     /gene="wzx"
     CDS             4453..5790
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAZ20759.1"
                     /db_xref="GI:71044439"
                     /translation="MNYLVNRKDIIWGYLSQFLNISSSLLLLPFILKCLNEEEIGLWY
                     VFVAMAGIIQLLEFGLLPTISRFISYVYSGALNLEYGKIPKCNIGDFNKELLNDVVVT
                     CRCIYFKIALFALVGILILGNAYIYTLSTSINKYYILCCWSVYGLSICIQLYFGYYNA
                     ILKGRGDQTLLNKIIVVAKLTLLLISIPLLIAGCGLVSLAVGAVISVIIDRILIRKYV
                     YSQESGLIRNLEKSNKTVSLKKVIWQSARDMGVVQLGNYLSVRAGVLIVSSFVNLDAA
                     ASYGLTVQVTMVIVIIASMFFGLNIPRITSEQAHENMLVVKNLMKKSLLIANCIYIFA
                     ALILVAIGDDILAFLTKNTTLLPKELLILYLVTALFEMNYSICTSYLTTRNEVIFIKS
                     MLYTGVAIVLFSFISTYIAEMGALGVILSQLLMQSLYNNWHWPLKVYQELKND"
     gene            5783..6907
                     /gene="wfaA"
     CDS             5783..6907
                     /gene="wfaA"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAZ20760.1"
                     /db_xref="GI:71044440"
                     /translation="MTNTKVKVIDLLRKNEEHYVFNSLSIKAIHEKYDTEIWVSEDST
                     YLDELDVGVKVNHVQVKRSKTYNWIISSFNLSKLILQNIGENTKIIVLSATPIQYTLI
                     SLISMMIPNNIYLFMHGELAYLKWSSCIGQKIGRLFIKIAMKMGRVKFICIGHSIQES
                     LAKIFPKRSFQYVQHPIVSNLCSKGLTVPLTFGSFGIHSQDKGSNKVYDLACCIENMH
                     FTNCKIITVGISDGSFKYDLHNKVSHYCKGNLKKQLIAKELFYEFLLKIDVALIFSNV
                     DEENDSKYDLISSGVLADCIAFGMPVIALKNKQLVSYFKKYGEFGFLCDTVNDMAVAV
                     RELSNNPKLILSFKDVLKKIRKDFEFEMFKSRMNEIINEK"
     gene            6897..8159
                     /gene="wzy"
     CDS             6897..8159
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAZ20761.1"
                     /db_xref="GI:71044441"
                     /translation="MKNKISFYIAYIYIAPIILSLYYISTTNSLLGDFRTYSYKLEWE
                     SSLLFLILYFIPLIIIFCVVKMTGKIKVDTFESIKLEKFAFYSIIITGLITLIFGANA
                     IGHPAQTGIAGVIIKIASKLNPLVLLPLLTFTNVSVRKFFFCAAVVIFYGYMQQSLQG
                     IYISVLCAGAFFLLKVRLNGAIFILMLLSPIIFLGPLLDIITHLYTIRNEIRGVSFSV
                     EEIFSLALGRISTTSSLIYTISNSFNTTGVSDYFVFGIVIERLIGVHILDTVSPSQVF
                     NTYILGANADYSIFMGLAGFLYFIKDFTCSVYCKCCVFDLGTDSYILGANADYSIFMG
                     LAGFFVFLLKTSPAVFIVNAACLILVLILIYLLIPVNNVKYRTSVFFLIMYLPFLSFD
                     IWEISIVFQTIIIWRVFLLLSKISFGLK"
     gene            8159..9235
                     /gene="wfaB"
     CDS             8159..9235
                     /gene="weiB"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAZ20762.1"
                     /db_xref="GI:71044442"
                     /translation="MKSILFVIPSMAPAGGIERVVSTIINKISKSYDCSILTFDDLPY
                     FYHVNDKIMKSSLNVDSVLDMKSRARRIIQVSKLILLAVIRLRKHFKNNNYDYVYTTH
                     PLNHCILLLAGINHKKIVIGEHGANNNYNLIYRLLKRVTYKYCHAYCLPTRSDYLFYR
                     EKGLPVKYTPHYKPELPYEMHKQNSKTILCVGRLTADKQHLLLLKMWKNIINEIPFGW
                     TLHIVGDGELKPILKDFINENGLSQSVRLSDSTKNISKYYIDSSFFALTSKSEGFGMV
                     ILEALSFGLPIISFDCPSGPRDMINDNNGFLIQPGDEVLYQNTLVKLIKHEKLRKSLT
                     LGALEFAANWNDDNITEHWRDIYK"
     gene            9232..10047
                     /gene="wfaC"
     CDS             9232..10047
                     /gene="wbgP"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAZ20763.1"
                     /db_xref="GI:71044443"
                     /translation="MNISVIIPVYNGAATIIEAIDSVIKQKIDGDITYNIEIIVVNDG
                     SQDNTLNILARYIEEIKAKNIKVINTENGGVSCARNIGIKAAKYEWVAFLDADDVWCE
                     NKLSIQIKHIKSIKSPVNFIGSARNHEELYLYGKKITSLYKVKPLDLLIKIFPQTSTA
                     LVKKEALLNCGLYDVNMTHCEDADLWIRICSLNGGFYYHPESTVITGGGKYNVGVSGL
                     SSDLKKMEAGICQMLRKTYLRGQISYFSYIFFYMFYKVKYIRRLTIVWIRRFM"
     gene            10044..10592
                     /gene="wfaD"
     CDS             10044..10592
                     /gene="wfaD"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl transferase"
                     /protein_id="AAZ20764.1"
                     /db_xref="GI:71044444"
                     /translation="MIFKIKWLFSALILSFFLKKISFPSFFAFPVFFKGLRRVSIGKH
                     VRIFPGCRFETHGTGEIIIEDDVSIAQNVHITSGTKLVIGQGTLITANSYITSIDHDY
                     ETIGVPIPRQHLDYKETRIGKNCFIGMGVAIQAGTILGEQCVVGANSVVRGVYPDYCV
                     IAGVPAKIIKVYDKNSGEWIRV"
     gene            10558..11553
                     /gene="wfaE"
     CDS             10558..11553
                     /gene="wfaE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAZ20765.1"
                     /db_xref="GI:71044445"
                     /translation="MIKTRENGFVSDVKNVLIFCPKFFNYDINIRNAVQKNGYTVSLF
                     NERPFDNIIGRALIRLGFDFFLKRQIFKYYKNIYDNIFADIDYLIVINPECITPEILE
                     IYRNKCNNIIVYMWDSFENKPQAKKLIRHADLFYTFDPNDAKNNNIIFKPLFYTKAYR
                     EIPSKPQLEYDISFIGTIHSSRYQYVKSIATVKNKFIFFYCPSLFVFLFKKYIAREIE
                     CIKLKDVSFKSLSESEVLNVIKRSRCILDVVHPKQNGLTIRTIEALGANKKIITTNRN
                     VVKYDFYNPSNILVLDDTVNDVAIAKFINQEYVHPDDEIYQSYYIENWVKDLLRE"
     gene            11557..12987
                     /gene="manC"
     CDS             11557..12987
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="AAZ20766.1"
                     /db_xref="GI:71044446"
                     /translation="MKIVPVVLAGGSGTRLWPLSRTMYPKQFLKIFGHMTMLQTTLSR
                     MNSFHDATDAIVICNEAHRFVVAEQLNQIDKKSTIILEPCGRSTAPAVALAAMHIVET
                     INADQDVLLLVLPADHIIKDLDTFHQAVKVASSFACGGKLVTFGIPPLTPETGYGYIQ
                     RGNSIGTVEGSECFEVSKFVEKPDVNTATQFLNDSNYYWNSGMFMFSARTYLEELQKY
                     CPDIVSVCQKSYDNSVCDLDFIRIDEKQFSNCLDLSIDYALMEHTNEAIVVPMDGDWS
                     DVGSWASLWDITNKDNNGNVLWGDILTRDSSNNYIYGESGLITTLGVEDLVIVQTKDA
                     LLVANRKSVQDIKQLVEQLKLRDRSEYYIHRETYRPWGKCDTIDKGKRYQVKRIEVKP
                     GEGISLQLHHHRSEHWVVVSGTAKITINGVEKIISENESIYIPLGAKHCLENPGKITL
                     ELIEIRSGSYLAEDDIIRFFDKYGRI"
     gene            12974..14368
                     /gene="manB"
     CDS             12974..14368
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="AAZ20767.1"
                     /db_xref="GI:71044447"
                     /translation="MVGYNKVNILMLICFKAYDIRGKLGEELNEDIAWRIGRAYGEFL
                     KPKTIVLGGDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIE
                     VTASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETTRGSYQQINLR
                     DAYIDHLFGYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPD
                     GNFPNGIPNPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVG
                     LLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAAGGIPVMSKTGHAFIKERMRKEDAIYG
                     GEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMAAFPASGEINSKLA
                     QPVEAINRVEQHFSREALAVDRTDGVSMTFADWRFNLRSSNTEPVVRLNVESRGDVPL
                     MEEKTQLILDLLRN"
BASE COUNT     4755 a   2430 c   3173 g   4779 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgagcagcg cgtgaagcgt
      121 caactgcttg cggaagtgca gtccatctgt ccaccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attttatgtg cacgacctgc tattggtgac
      241 aatccatttg tcgtggtgct gccagacgtt gtgatcgatg acgccagcgc cgacccgctg
      301 cgctacaacc ttgcggccat gattgcgcgc ttcaacgaaa cgggccgcag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca ttcagaccaa agaaccgctg
      421 gatcgtgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagatattat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcagcc tggtgcatgg ggacgtattc agctgactga tgccatcgct
      601 gaactggcga aaaaacagtc cgttgatgcc atgctgatga caggtgacag ctacgactgc
      661 ggtaaaaaaa tgggttatat gcaggcgttt gtgaagtatg gactacgcaa cctcaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ctgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcgaa agtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagga taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgtcacatc gtaggcatgc
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgcgttaata cctctattaa
     1081 tcaaactgag agccgcttat ttcacagcat gctctgaagt aatatggaat aataaagtga
     1141 agatacttgt tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa
     1201 ataatactca ggatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctggaat
     1261 cacttgctga tgtttctgat tctgaacgct atgtctttga acatgcggat atttgtgata
     1321 cagctgcaat ggcacggatt tttgctcagc atcagccgga tgcagtgatg cacctggcag
     1381 ctgaaagcca tgttgaccgt tcaattacag gccctgcggc atttattgaa accaatattg
     1441 ttggtactta tgtcctttta gaagcggctc ggaattactg gtctgcactt gatggcgaca
     1501 agaaaaacag cttccgtttt catcatattt ctactgacga agtctatggt gatttgcctc
     1561 atccagatga agtaaataat acagaagaat tacccttatt tactgagacg acagcttacg
     1621 cgccaagcag cccttattcc gcatccaaag catccagcga tcatttagtc cgcgcgtgga
     1681 aacgtaccta tggtttaccg accattgtga ctaattgctc taacaattat ggtccttatc
     1741 atttcccgga aaaacttatt ccattggtta ttctcaatgc tctggaaggt aaagcattac
     1801 ctatttatgg taaaggggat caaattcgcg actggttgta tgttgaagat catgcgcgtg
     1861 cgttatatac agtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca
     1921 acgaaaagaa aaacatagat gtagtgctca ctatttgtga tttgttggat gagattgtac
     1981 cgaaagagaa atcttatcgc gagcaaatta cttatgttgc cgatcgtccg ggacacgatc
     2041 gccgttatgc gattgatgct gagaagattg gtcgcgaatt gggatggaaa ccacaggaaa
     2101 cgtttgagag cgggattcgg aagacagtgg aatggtattt gaataatagt gattggtgcc
     2161 gacgagttca agatggctct tatcaacgtg agcgtttggg gttagttaaa tgaaaggaat
     2221 tattttagcc ggtggttctg ggacaaggct ttatccaatt acaatggggg tttcaaagca
     2281 gctattgccc atttatgata aaccgatgat ttattatccg ttatcggtgt tactattagc
     2341 aaaaatcagg gatatactga taataacaac accggaagat catgcttctt ttatcagatt
     2401 attaggcgat ggttctcgat ttggtattaa cttaagttat gctgttcaag aaaaaccaga
     2461 aggattagct caggcatttt taataggttc tgaatttatt ggggctgata aggtttgctt
     2521 agtgctagga gataatatct tttttggaca agcatttggc aagcaattat ctaaagtgat
     2581 aaaagagtta gaaggtgcca ctgtatttgg atataaagta cgagacccag aaagatttgg
     2641 tgtggttgaa tttgataatg ataataaagc tgtatcaata gaagaaaaac ctgttaaagc
     2701 aaaatcaaat tgggcagtaa caggattata tttttatgat aatacagttg tggatatagc
     2761 gaagaatgta aaaccttcag aaagaggtga gttagaaata acctcgatta acgaaacata
     2821 cctaagtcaa ggaaaactaa aagttgaaca attagggcga ggatttgcgt ggcttgatac
     2881 tggaactcat gattctctat tagacgcttc gcattttgta gagactgttg agaaaagaca
     2941 aggttataaa atagcatgtc tggaggagat agcatataat aataattggt taactaagga
     3001 ggatatacgg tttcttgcaa aaaaactaac aaaaacagat tatgggaaat atttattgga
     3061 gttgattaaa tgaatgttat taaaacaaaa atagaagatc ttgttattat tgagccaaaa
     3121 atatataatg atgaaagagg ttatttttat gagttttata ataaaaataa atttactgag
     3181 cttggtataa atctagattt tgtacaagat aatagatcaa gttcgtcaca aaacgttctg
     3241 agaggtctac attttcaaaa aacaaaacct cagggaaaac tcgttacggt aaccaaaggt
     3301 gaagtatttg atgttgcagt tgatttacgt aaagattcac gaacatttgg gcaatatgaa
     3361 tcagttattc tgacagagga aaataaaaaa atattttata taccaccagg ctttgcacat
     3421 ggattttgtg tattaagtaa taaggctgat tttttataca aatgtactga cttttatgac
     3481 ccaaatgatg aaggggggat tatatggaat gactcttcat tatcgattga atggcctgtt
     3541 aatgctccta tattgtctaa aaaagacatg gagcttccaa cattaaaaaa tattagacaa
     3601 tcgttattgg atggatttta aaatgtcgca gtttacaatt tttggtggtt ctggatttat
     3661 aggttctgaa atagtaaaaa agttaaaatc agaaaaaaaa gatgtatatg tgccaaggcg
     3721 tgatgaagac ttatgcttcg gtaaagattt aggaatagtt atatactgtg caggaaatgg
     3781 tgattgtgta aatacgccta gaagtgttta cgatgcaaat ataaggtatt taactgatat
     3841 tttatttgaa tgtaattata aagtacttgt ctatgtatcc tcaactaggg tttatataaa
     3901 taatgactct tcattagaag gcaatgatat catagttaca tcttgtgatg agcgtagatt
     3961 atttaatctt acaaagttgg tatctgaaga actttgtaac cgaagtggta aaccagtaat
     4021 tatcgtgagg ccgagcaatg tttatggcct ggcaataaaa agccctctat tcttacccac
     4081 aataacaaga aatgcaatac ttaatggaag tgttgatatg tttgttgagg aagaatacga
     4141 gaaagattat gtgtcagtta atgatgtagt tgattgtatt attaagttaa gtatgatgga
     4201 taaggctatt ggaaaaacta tcaatattgc atcaggctat aatatagcag caaaagattt
     4261 agcggatatt cttcgaaata aaaccaattg tattattaat tggcatggta attcaggtaa
     4321 acgagaaaac tttccgatta ttaatataga attattgaag agttttattc ctgaatacaa
     4381 acctcgttct gttttagctg atctcgaata tatgattaaa gagtttaaac ataacattga
     4441 ctcaaaagta ttatgaatta tttagtgaac aggaaagata taatttgggg gtatctatca
     4501 cagttcttaa atatatcttc aagtttattg cttcttcctt ttatattgaa atgccttaat
     4561 gaggaagaaa taggtctatg gtatgttttt gttgcgatgg caggaataat acagctttta
     4621 gaatttggcc tattaccaac gatttcaagg tttatatcat atgtatatag tggcgcacta
     4681 aatttagagt atggaaaaat tcctaaatgt aatataggtg atttcaacaa agagttacta
     4741 aatgatgtgg ttgtgacctg taggtgtatt tattttaaga tcgctttatt tgctcttgtt
     4801 ggaattttga tacttggtaa tgcatatata tatacattgt caacatcgat taataaatat
     4861 tatatacttt gctgttggtc ggtttatggc ttaagtattt gtattcaact atactttggt
     4921 tattataatg caatattaaa aggtcgaggt gatcaaacat tattaaataa aataattgtc
     4981 gtcgctaaat taacgttact gttgattagt ataccattgt taatcgctgg atgtgggtta
     5041 gtttcactag ccgttggagc tgtaatatct gtaattattg ataggatctt aattcggaaa
     5101 tatgtgtact ctcaagaaag tggattaatt cggaacttag aaaaaagcaa taaaacagta
     5161 tcgttaaaaa aagtcatttg gcaaagtgca agagatatgg gggtagttca gcttggcaat
     5221 tatttaagtg tgcgggcggg ggtattgata gtctcaagct ttgtgaattt ggatgctgct
     5281 gcaagttatg gtttaaccgt ccaagttact atggttattg tgataattgc atctatgttc
     5341 tttgggttaa acatacccag aattacatca gagcaagcac atgagaatat gttagttgtt
     5401 aagaatttaa tgaaaaaatc attgctcatt gctaattgta tctatatttt tgccgcttta
     5461 attcttgttg cgatcggaga tgatattctc gcttttttaa caaaaaatac gactttgctc
     5521 ccaaaagaac tcctcatttt gtaccttgtc acagcgttgt ttgaaatgaa ttactctatc
     5581 tgcacatcct acttgacgac taggaatgaa gtgatattta taaaaagtat gttgtacact
     5641 ggcgttgcaa ttgttttgtt ctcatttata tcaacgtaca ttgcggaaat gggagcgtta
     5701 ggggttatat tatcacaatt attaatgcaa tcattatata ataattggca ttggccatta
     5761 aaagtatatc aggaattaaa gaatgactaa tactaaagta aaagtaattg atctgcttcg
     5821 aaaaaatgaa gaacattatg tttttaatag cctttcaatt aaagcaatac atgagaaata
     5881 tgacactgag atatgggtat ctgaagattc aacttatttg gatgaactgg atgtaggcgt
     5941 taaagtcaat catgttcaag ttaaaagaag taaaacatat aattggataa tatcatcatt
     6001 taacttgtca aaattgattt tgcaaaacat aggagagaac actaaaataa ttgtcctgtc
     6061 tgcgactcca atacaatata cattaattag tctaattagt atgatgattc ctaataatat
     6121 atatttattt atgcatggtg aattggcgta tttaaagtgg agttcttgta ttggtcaaaa
     6181 aataggtcgt ctatttataa aaatagccat gaaaatggga cgggtaaaat ttatttgcat
     6241 cggtcattct atacaagaaa gcttagcaaa gattttccct aaaagatcat ttcaatatgt
     6301 ccagcatcca attgtttcta atttgtgttc caagggctta actgttccac ttacatttgg
     6361 tagttttggt attcactcac aggataaagg tagcaataaa gtatacgatt tggcatgttg
     6421 tattgaaaat atgcatttta ccaattgtaa aataattacc gttggcataa gcgacggaag
     6481 ttttaaatat gatttgcata ataaggtgag tcactattgc aaaggtaatt tgaagaaaca
     6541 gctaatagct aaggagctgt tttatgagtt tttgttaaaa attgatgtgg cgttaatttt
     6601 ttcaaatgtt gatgaggaaa atgatagtaa atatgacctt atctcaagcg gtgtacttgc
     6661 agattgcatt gcatttggta tgcctgttat tgctttaaaa aataaacagt tagttagcta
     6721 ctttaaaaag tatggtgagt ttggatttct ttgtgacact gtaaacgata tggctgttgc
     6781 tgttagagaa ttatcgaata accccaagct tatattatca ttcaaagatg ttctgaaaaa
     6841 aataagaaaa gactttgagt tcgagatgtt taaatcaaga atgaatgaga taattaatga
     6901 aaaataagat atcattctat attgcatata tatatatagc accaataata ttatcgttgt
     6961 actatatttc aacaacaaac tcattattag gtgattttcg aacttatagt tataaacttg
     7021 aatgggagtc ttcattatta ttcttaatat tgtatttcat ccctttaatt atcatatttt
     7081 gtgttgtgaa aatgactgga aaaataaagg ttgatacatt tgagagtatt aaacttgaaa
     7141 agtttgcatt ttattcaatt attataacag gtctgattac tcttatattc ggggcaaatg
     7201 ccataggcca tcccgcacaa acaggtatag ctggtgtaat tataaaaata gcgagcaaat
     7261 taaatccact tgtgttattg ccattattaa cattcacaaa tgtgtcggta agaaaatttt
     7321 ttttctgtgc tgcagttgtt attttttatg gatatatgca acaatctttg caaggtatat
     7381 atattagcgt tctttgtgcg ggggcgtttt ttttactgaa agttagattg aatggcgcca
     7441 tattcatcct aatgcttttg tcgccaatta tatttttggg gccacttctt gatattataa
     7501 cacacctata tacaattaga aatgaaattc gtggcgtttc gtttagtgtt gaagaaattt
     7561 tttcactagc acttggccgt atatcaacca cgtcatcatt aatatatact attagtaata
     7621 gttttaatac tacaggagtt tcggactact ttgtttttgg tattgtgatt gaacgtctaa
     7681 ttggggtgca tatactggat acagtttctc cttctcaagt attcaataca tatatacttg
     7741 gcgctaatgc tgattatagt atttttatgg gcttggcagg ttttttgtat tttattaaag
     7801 acttcacctg cagtgtttat tgtaaatgct gcgtgtttga tcttggtact gattcttata
     7861 tacttggcgc taatgctgat tatagtattt ttatgggctt ggcaggtttt tttgtatttt
     7921 tattaaagac ttcacctgca gtgtttattg taaatgctgc gtgtttgatc ttggtactga
     7981 ttcttatata cttattgatt ccagttaaca atgttaaata taggacatct gttttttttc
     8041 ttataatgta tcttccattt ttatcatttg atatttggga gatttccata gtttttcaaa
     8101 caattattat ctggcgagta tttcttttgc tatctaagat ttcttttggg ctaaaataat
     8161 gaaaagtatt ttattcgtaa ttccttctat ggcaccagcg ggagggatag aaagagttgt
     8221 tagcacgata attaataaaa taagtaaatc atatgattgt tcaatcttga cttttgacga
     8281 tttaccttat ttttatcatg tcaatgataa aataatgaaa agtagtctaa atgttgattc
     8341 tgttcttgat atgaagagta gagctagaag aattatccaa gtatctaagc taatactttt
     8401 agcggtaata aggctgagaa agcactttaa aaataataat tatgactatg tatatacgac
     8461 acatccttta aatcactgta ttctattact ggcaggaata aatcataaga aaattgtcat
     8521 tggtgaacat ggcgctaata ataactataa cttgatttat cgattattaa aaagagtgac
     8581 ttataaatat tgccatgctt attgtcttcc aacaaggagt gattatctgt tttacagaga
     8641 aaaaggattg ccagttaaat atacgcctca ttacaaacca gaactgcctt atgaaatgca
     8701 taagcaaaat agcaaaacga tattgtgtgt tggaagacta acagctgata aacaacatct
     8761 gttgttatta aagatgtgga aaaatataat taatgaaatc ccatttggat ggacacttca
     8821 tattgttggt gatggagagt tgaaaccaat tcttaaagat tttattaacg aaaacggact
     8881 tagtcagtca gtgagattaa gtgattcgac gaagaatatt agtaaatatt atattgattc
     8941 atcttttttt gcattaacat caaaaagtga aggatttggg atggtgatac ttgaagctct
     9001 ttcatttggg ttacctatca tttcattcga ttgcccttct ggtccgagag acatgattaa
     9061 tgataataat ggatttctta ttcagccagg agatgaagta ttgtatcaaa atactcttgt
     9121 aaaattaatt aagcatgaaa aattaagaaa gagcttaacc ttaggtgccc ttgaatttgc
     9181 tgcgaattgg aatgatgata acataacaga acattggcgg gacatctata aatgaatata
     9241 tcagtaataa ttcctgttta taatggtgct gcaaccatca tagaggcaat tgactcagta
     9301 atcaaacaaa aaatagatgg agatataaca tacaatattg aaataattgt agttaatgat
     9361 ggctcgcaag ataatacgct aaatatttta gctcgttaca tagaggaaat aaaagctaaa
     9421 aatattaagg ttataaatac agaaaatggt ggggtttcgt gtgcaagaaa tattggtatt
     9481 aaagctgcga aatatgaatg ggttgctttt cttgatgctg atgatgtttg gtgtgaaaat
     9541 aaattaagca ttcagataaa acatatcaaa tccattaaat ctcctgttaa ttttattggc
     9601 tcagctcgta atcatgaaga gctatattta tatggtaaaa aaataacttc attatataaa
     9661 gttaaacctc tggacctatt gattaaaatt ttccctcaaa cttcaactgc attagtaaaa
     9721 aaggaggctt tgcttaattg tgggttatac gatgttaata tgactcattg tgaggatgca
     9781 gacttatgga tcaggatatg ctcactgaat ggtggttttt attaccatcc agaatctaca
     9841 gttattacag ggggaggaaa gtataatgta ggtgtgtctg gcttgtcatc agatttaaaa
     9901 aaaatggagg ccggaatctg ccaaatgtta agaaaaacat atttgcgggg tcagatatca
     9961 tacttttcat acatattttt ttatatgttt tacaaagtta aatatataag aagattgaca
    10021 attgtgtgga ttaggaggtt tatgtgatct tcaaaattaa gtggttattt tcagcgttaa
    10081 tactttcttt ttttctgaaa aaaatatcat tcccatcatt tttcgctttt cctgtttttt
    10141 ttaaaggctt gagaagagtt tccattggta aacatgttag aatatttccg gggtgtcgtt
    10201 tcgagactca tggtaccggt gaaatcatca tagaggatga tgtttcaatt gcccaaaatg
    10261 tacatattac atcaggtacc aaactagtta tcgggcaagg gacgttgata actgctaata
    10321 gttatattac atcaatagat catgattatg aaactatcgg agttcctatc ccacgtcagc
    10381 atctagatta taaagagaca cgtattggta aaaattgctt tatcggtatg ggggttgcta
    10441 ttcaggccgg tacaattctt ggagaacaat gtgttgtcgg tgctaactct gttgtcagag
    10501 gagtttaccc tgactattgt gtaatagcag gtgtaccagc gaagattata aaagtttatg
    10561 ataaaaactc gggagaatgg attcgtgtct gatgtaaaga atgtactgat tttttgccca
    10621 aagtttttta attacgacat taatattcgg aatgctgtcc aaaaaaatgg atatactgta
    10681 agcttattta atgagcgccc ttttgataat ataattgggc gtgctttaat aagactgggt
    10741 ttcgattttt ttttgaagcg tcagattttt aaatattaca aaaatatata tgacaatatt
    10801 tttgcagata tagattattt gattgtgata aatcctgaat gtataacccc tgaaatattg
    10861 gagatttatc ggaataaatg caataatatc attgtatata tgtgggattc atttgaaaat
    10921 aaaccgcagg caaaaaaact catacgacac gctgacttat tttatacatt tgatccaaat
    10981 gatgcaaaaa ataacaacat tatatttaaa ccactttttt atacaaaagc atatcgtgaa
    11041 atcccttcta aacctcaact tgaatacgat atttctttca ttggtactat tcattcatct
    11101 cgctatcaat atgttaagtc tatagcgaca gtaaaaaaca aatttatatt tttttattgt
    11161 ccaagtcttt ttgttttttt atttaaaaaa tatattgcaa gagagattga atgtataaaa
    11221 ttaaaggacg tttcttttaa atctttaagt gaaagtgagg ttttaaatgt tatcaaaaga
    11281 tcaaggtgta tactcgatgt tgttcatcca aaacagaatg gactaacaat aagaactatt
    11341 gaagcgcttg gggccaataa aaaaatcatc acaactaatc ggaatgtcgt taaatatgat
    11401 ttttataatc cttcaaacat attagtttta gatgacacag tgaatgatgt tgctattgct
    11461 aagtttatta atcaagagta tgtacatcct gatgacgaaa tataccagag ttactatatc
    11521 gaaaactggg ttaaggactt attgagagag taaattatga agattgttcc tgtagtatta
    11581 gctggaggta gtggtacaag actttggcca ttatcgagaa cgatgtatcc taaacaattt
    11641 ctgaaaatat ttggtcacat gaccatgtta caaaccactt tatctagaat gaatagtttt
    11701 catgatgcaa ctgatgctat cgttatatgt aatgaagctc accgtttcgt ggttgctgaa
    11761 cagctaaatc agattgataa aaaaagtact atcattcttg aaccttgtgg caggagtaca
    11821 gctcctgctg tcgcgttagc tgctatgcat attgtcgaaa ctatcaacgc tgatcaagac
    11881 gtgctattgt tagttctccc ggcagaccat ataataaaag atctagacac ctttcatcag
    11941 gccgtaaaag tagctagctc ctttgcatgt ggtgggaaac ttgtaacttt tggcattcct
    12001 cctctaacac ctgagaccgg atatggttac attcaacgtg gtaatagcat tggcaccgta
    12061 gagggaagtg aatgctttga ggtttctaaa tttgtcgaaa agccagacgt aaatactgca
    12121 acgcaatttt taaatgattc caattactat tggaatagtg gtatgtttat gtttagcgcc
    12181 cgaacttatc ttgaagaatt acaaaaatac tgtcctgata ttgtgagtgt atgtcagaag
    12241 tcttacgata attctgtctg tgacttggat tttatccgta ttgatgaaaa acaattctcg
    12301 aattgtctgg atttatctat tgattatgct ttaatggagc atactaatga ggccatagtt
    12361 gtacctatgg atggtgattg gagtgacgtg ggctcttggg catcattatg ggatataact
    12421 aataaagata ataacggaaa tgttctttgg ggcgatatac ttactagaga tagttcgaat
    12481 aattatatct acggtgaatc aggacttatt acaactctgg gcgttgaaga tttagtcata
    12541 gttcaaacaa aagatgctct tcttgtagct aatcgaaaat ctgttcagga tattaagcaa
    12601 cttgttgagc aattaaagtt acgcgacaga agtgaatatt atattcatcg cgagacttac
    12661 cgaccttggg gtaaatgcga taccattgat aaaggcaaaa gataccaagt gaagagaatt
    12721 gaggttaagc ctggtgaagg aatatcgttg caacttcatc atcatcggtc tgagcactgg
    12781 gtagttgtct ctggtaccgc aaagattaca attaatggcg ttgaaaaaat aatcagtgaa
    12841 aatgaatcca tctatatacc actaggtgcc aagcactgtt tggaaaaccc tggaaaaatc
    12901 acactcgaat taatcgagat acgttcagga agttatcttg ctgaagatga cataattcga
    12961 ttttttgata aatatggtag gatataacaa ggttaatata cttatgctaa tttgttttaa
    13021 agcctatgat attcgcggaa aattaggcga agaactgaat gaagatatcg cctggcgcat
    13081 tggtcgcgcc tatggcgaat ttcttaaacc gaaaaccatt gtgttaggcg gtgatgtccg
    13141 cctcaccagc gaaaccttaa aactggcgct ggcaaaaggt ttacaggatg cgggcgtcga
    13201 tgtgctggat atcggcatgt ccggcaccga agagatctat ttcgccactt tccatctcgg
    13261 cgtcgatggc ggtatcgaag tcaccgccag ccataatccg atggattaca acggcatgaa
    13321 gctggtgcgc gaaggtgctc gcccgatcag cggcgatacc ggactgcgcg acgtccagcg
    13381 tctggcagaa gccaacgact tcccgcctgt cgatgaaaca acacgcggta gttatcagca
    13441 aatcaacctg cgcgacgcct acattgatca cctgttcggc tatatcaacg tcaaaaacct
    13501 cacgccgctc aaactggtga tcaactcagg gaacggcgca gcgggtccgg tggtggacgc
    13561 cattgaagcc cgctttaaag ccctcggcgc acctgtggaa ttgatcaaag tgcacaacac
    13621 gccggacggc aatttcccca atggtattcc taatcctttg ctgccggaat gtcgcgacga
    13681 cactcgcaat gcggtcatca aacacggtgc ggacatgggc attgcctttg atggcgattt
    13741 tgaccgctgc ttcctgtttg acgaaaaagg gcagtttatc gagggctact acattgtcgg
    13801 tttgctggca gaagcgttcc tcgaaaaaaa tcccggcgcg aagattatcc acgatccgcg
    13861 tctttcctgg aacactgtcg atgtggtgac tgccgcaggc ggcataccgg tgatgtcgaa
    13921 aaccggacac gcctttatta aagaacggat gcgcaaggaa gacgccatct acggtggcga
    13981 aatgagcgcg caccactact tccgtgattt tgcttactgc gacagcggca tgatcccgtg
    14041 gctgctggtc gctgaactgg tgtgcctgaa agagaaaacg ctgggcgaac tggtgcgcga
    14101 ccggatggcg gcgttcccgg caagcggtga gatcaacagc aaactggcgc aacccgttga
    14161 ggcgattaac cgcgtggaac agcactttag ccgcgaggcg ctggcggtgg atcgtactga
    14221 tggcgtcagc atgacctttg ccgactggcg ctttaacctg cgctcttcca acaccgaacc
    14281 ggtcgtacgc ctgaatgtgg agtcgcgcgg tgatgtgccg ctgatggaag aaaaaacaca
    14341 attaattctt gatttactca gaaattaagt agactgaaga atgagcggta cacaggttca
    14401 tatataaaat gttaatgcta tgagtgtgat aaagcatatg tacctctatt catcatgact
    14461 ctgtctgagt taacatctgc atcacattca agccgcgcaa ccccgcggag accaccactg
    14521 acaggagtaa acaatgtcaa agcaacagat cggcgtcgtc ggtatggcag tgatgggacg
    14581 caacctcgcg ctcaacatcg aaagccgtgg ttataccgtc tctattttca accgctcccg
    14641 tgaaaagacg gaagaagtta ttgccgaaaa tccaggcaag aaactggttc cttactatac
    14701 ggtgaaagag ttcgttgaat ctcttgaaac gcctcgtcgc atcctgttaa tggtgaaagc
    14761 aggtgcgggc acggatgctg ctattgattc cctgaaacca tatctcgata aaggcgatat
    14821 catcattgat ggtggtaata ccttcttcca ggacaccatt cgtcgtaacc gcgagctttc
    14881 tgcagaaggc tttaacttca tcggtacggg tgtttccggt ggtgaagagg gcgcgctgaa
    14941 aggaccttct atcatgcctg gtgggcagaa agaagcctat gaactggttg ctcctatcct
    15001 gaccaaaatc gccgccgtgg ctgaagatgg cgaaccgtgc gttacctata ttggtgccga
    15061 tggcgcaggt cactatgtga agatggttca caacggtatt gaatacggtg atatgcaact
    15121 gattgctgaa gcctatt
//
