LOCUS       AB812027               13294 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: P7d.
ACCESSION   AB812027
VERSION     AB812027.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 13294)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..13294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="P7d"
                     /db_xref="taxon:562"
                     /note="serogroup:O68"
     gene            <1..908
                     /gene="wcaM"
     CDS             <1..908
                     /gene="wcaM"
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01146.1"
                     /translation="NYAILRQGFHNXIIGANITNCKFSDLQGDAIEWNVAINDSDILI
                     SNHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVE
                     NGKHFVIRNIKACNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVI
                     KGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNK
                     PQHLFMRNINVMQESSVGAALRMNFDMRKDVRGVFMAKEETLLSLANVHAVNERGQSS
                     VDIDRINHHIVNVEKINFRLPERRE"
     gene            1071..1976
                     /gene="galF"
     CDS             1071..1976
                     /gene="galF"
                     /note="O068_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01147.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLESDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            2348..3433
                     /gene="rmlB"
     CDS             2348..3433
                     /gene="rmlB"
                     /note="O068_02"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAQ01148.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPHAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            3433..4332
                     /gene="rmlD"
     CDS             3433..4332
                     /gene="rmlD"
                     /note="O068_03"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="BAQ01149.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIALALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            4390..5268
                     /gene="rmlA"
     CDS             4390..5268
                     /gene="rmlA"
                     /note="O068_04"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAQ01150.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSSRGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            5273..5815
                     /gene="rmlC"
     CDS             5273..5815
                     /gene="rmlC"
                     /note="O068_05"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="BAQ01151.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHFQRPPFEQAKLVRCIVGEVFDVAVDIRVGSDSYGHWVGII
                     LSSENKRQLWIPEGFAHGFLVLSDEAEFVYKTTNYYCPEFEAGISWCDGFLNINWPQL
                     DEIKLSLKDKQWGGINAFKS"
     gene            5830..7041
                     /gene="wzx"
     CDS             5830..7041
                     /gene="wzx"
                     /note="O068_06"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01152.1"
                     /translation="MLKNIFFLFLIQISNYIFPLLTLPYLVRVLGASQFGSLMLAQAL
                     IQYFITVTEYGFNLSATKKIALSKSQDETNKIYTNTLYARIILALICFIIAILCTQFI
                     EMFEREFNVIIILFLGVIGNCFFPIYLFQGLELMKKIAWISIVSKTFMTLSVFVLVKD
                     AKDINNAALALAIPLLLPGIISFIYIYNRQLARVVSFSFRGIVKELKDGGALFISQIA
                     ISFYTTFNTLILGYIFNPTIVGVFSAADKLRIAAQSCYVPIQQXVFPRINKEAGELST
                     KLLKYGGFFVLISFLGSSLVFFFGEQLALLYLGNEFIISARLFKWMSLLLFIISIAIV
                     FGQWGLITLGKEKLLTKIYVIGALCHVGYSIPLVMGYNVYGMLVSVLITEFIITLMMI
                     FSFYKVTHSAI"
     CDS             7049..7999
                     /note="O068_07"
                     /gene="wfcB"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01153.1"
                     /translation="MVYIIVLNWNNSEDTISCISTLLKQNYIYFRIVLVDNDSTDDSL
                     IKILAFLKKSCQNYLHLSESESYLDEEIHTNKITVISATSNKGYAGGNNIGIKYALQS
                     NDCKYIWVLNNDTEVESNSLSLLVSEMERDKEIGILGSKLVYYHNKNVIQGLGGKYLP
                     WRFLTQHIASNHLITSKYDNDTISKQIDYIIGAALFVKAEVFKTVGLLDEQYFLYFEE
                     LDICLRARKHNYKLKTITDSIVYHKEGATISKEKNAFSEYHYLRSNKLFIRKFYPQTL
                     FLYNMMLIAKIANRVRRGQFDLAKANLKVLLGIKYVNSKT"
     CDS             8001..9068
                     /note="O068_08"
                     /gene="wceA"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01154.1"
                     /translation="MKIDNMGNNIKKVLFFNPLKKIMIRGISVYSGEYEQLLRDKNVE
                     VIVYELPLWFGKMPKAMQVFGFMFAQQVLLPIRALKVKPDLIFDAYNTYSILAALKFN
                     YIFVIHDFIPFNNKLWFLKPGALYQKILHNLSKNFSKLHLCYINSRIINDKNAIIKKK
                     YFILPNIVRSLGYHKVSNVEIENFIKAGRELDAICIVTISGSGKNKDFGGLVERLNNL
                     NVPVYLVAFGFANNFCEEFNNVNVFSPGVVDSNLIGSVITNSDLFVFHSTSEGFGRPI
                     IEALELKAKVICSDVSPSLDYINEEYLPNLYIYSLRDMNEFKYQFDKALRTPKRLIQS
                     DSVLTYNDEDLFNEISRIIEA"
     gene            9070..10176
                     /gene="wzy"
     CDS             9070..10176
                     /gene="wzy"
                     /note="O068_09"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01155.1"
                     /translation="MLYLTNLIFLYQIILTVLLFLPSSIFSGLIIEYTIPLLFIFVMS
                     ATIYFITKRGNSFKYCQISICLPSKLILQCFFFFFIALNFSEFIFVATHGGHQALYMA
                     DRPLFPFLIFKLLAFPAFYFATIIILTKNYVVSRLLVWLALFSPLLTGSRGMTIFLLL
                     AYLFVTFGPFFFIRFKFAILGFLMIISFIGIGYYREPINMSIADYIILVVSSLNEFAF
                     ASMNISNCQIPFSNVFTQFGDIFLGAKSDFRVATLLTECVSPGASEKGYGISSSIVGE
                     SKILGDNLWFLYFIAILSINSIMISILLRSGSRLSHMVGISLLPFILYSVRTEIVYPY
                     VFLIKILIYILIAMIINQILVKSSAVEGANKFLI"
     CDS             10217..11197
                     /note="O068_10"
                     /gene="wbaD"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01156.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMVEVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
     gene            11255..12253
                     /gene="wzz"
     CDS             11255..12253
                     /gene="wzz"
                     /note="O068_11"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01157.1"
                     /translation="MFAGKKSAQIREILISESAWEEMTCLFAPSLVQLWRGKMTIIIS
                     VIVAIALAIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETL
                     IGRFSSAFSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQ
                     QVDDKVNQELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQ
                     VTKPQVQQTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLRV
                     DDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12351..12962)
                     /gene="hisI"
     CDS             complement(12351..12962)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01158.1"
                     /translation="MLTEQQCRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEAVDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     3834 a   2537 c   2969 g   3952 t
ORIGIN
        1 ctaattacgc catcttacgc cagggatttc ataaccngat tatcggtgcc aacatcacca
       61 attgtaagtt cagcgactta cagggcgacg ccattgaatg gaacgtggca attaacgaca
      121 gtgatatttt gatctccaac catgtcatcg agcgcatcaa ctgtactaac ggcaaaatca
      181 actggggcat tggcataggc cttgcgggaa gcacttacga taataattac ccggaagatc
      241 aggcagtgaa aaactttgtc gtggcgaata tcacgggatc ggattgtcgg cagttgatcc
      301 atgttgaaaa tggtaaacat tttgttattc gtaatatcaa agcttgcaat atcacgccgg
      361 atttcagtaa gaaagcaggc attgataacg ccacagtcgc tatttacggt tgtgacaatt
      421 tcgtgattga taatattgaa atgattaata gcgccgggat gttaatcggt tatggggtaa
      481 ttaaaggcaa atatctctcg ataccgcaaa atttccgagt gaataatatt caactggata
      541 acacccatct tgcttacaaa ttgcgcggta tccaaatctc cgccgggaat gctgtctcct
      601 ttgtggcact gactaacatt gagatgaagc gtgcgtcgct ggagttacac aacaaaccgc
      661 aacatctttt tatgcgtaat atcaatgtga tgcaggaatc ctcagttgga gccgcattgc
      721 gcatgaactt cgacatgcgc aaagacgttc gcggcgtctt tatggcgaaa gaagaaacac
      781 tgctgtctct tgcaaatgtt catgcggtga atgaaagagg gcaaagctcc gtcgatatcg
      841 acaggattaa tcaccatatt gttaatgtgg aaaagattaa ctttagattg ccggaacgga
      901 gggagtagat ttgcgaccat tcctggaaaa atggagccat acttaggaac aatgctactg
      961 caatccacaa cgaagcggcg taacatcaca agtaattcag taatcaattc agggtaattg
     1021 atgctggcga aaaaaatcga acaagctata attcagcaac cattttacag gtggatgaaa
     1081 taatgacgaa tttaaaagca gttattcctg tagcgggtct tgggatgcat atgttgcctg
     1141 ccactaaggc gattcccaaa gagatgctac cgatcgtcga caagccaatg attcagtaca
     1201 ttgttgacga gattgtggct gcagggatca aagaaatcct cctggtaact cacgcgtcca
     1261 agaacgcggt cgaaaaccac ttcgacacct cttatgaatt agaatctctc cttgagcagc
     1321 gcgtgaagcg tcaactgctg gcggaagtgc agtccatctg tccaccgggc gtgaccatta
     1381 tgaacgtgcg tcagggcgaa cctttaggtt taggccactc cattttatgt gcacgacccg
     1441 ccattggtga caatccattt gtcgtggtgc tgccggacgt tgtgatcgac gacgccagcg
     1501 ccgacccgct gcgctacaac cttgctgcca tgattgcacg attcaacgaa acgggtcgta
     1561 gccaggtgct ggcaaaacgt atgccgggtg acctctctga atactcggtc atccagacca
     1621 aagaaccact ggaccgtgaa ggtaaagtca gccgcattgt tgaatttatc gaaaaaccgg
     1681 atcagccgca gacgttggag tcagacatca tggccgttgg tcgctatgtg ctttctgccg
     1741 atatttggcc ggaacttgaa cgcacgcagc caggtgcatg ggggcgtatt cagctgactg
     1801 atgccatcgc tgaactggcg aaaaaacagt ccgttgatgc catgctgatg acaggtgaca
     1861 gctacgactg cggtaaaaaa atgggttata tgcaggcatt tgtgaagtat ggactacgca
     1921 acctgaaaga aggggcgaag ttccgtaaag gcattgagaa gctgttaagc gaataatgaa
     1981 aatctgaccg gatgtaacgg ttgataagaa aattataacg gcagtgaaga ttcgtggcga
     2041 aagtaatttg ttgcgaattt tcctgccgtt gttttatata aacaatcaga ataacaacga
     2101 gttagcaata ggattttagt caaagttttc caggattttc cttgtttcca gagcggattg
     2161 gtaagacaat tagcgtttga atttttcggg tttagcgcga gtgggtaacg ctcgtcacat
     2221 catagacatg catgcagtgc tctggtagct gtaaagccag gggcggtagc gtgcattaat
     2281 acctctatta atcaaactaa gagccgctta tttcacagca tgctctgaag taatatggaa
     2341 taaaagagtg aagatacttg ttactggtgg cgcaggattt attggttctg ctgtagttcg
     2401 tcacattata aataatacgc aggatagtgt tgttaatgtc gataaattaa cgtacgccgg
     2461 aaacctggaa tcacttgctg atgtttctga ttctgaacgc tatatttttg aacatgcgga
     2521 tatttgcgat gctgctgcaa tggcacggat ttttgctcag catcagccgc atgcagtgat
     2581 gcacctggca gctgaaagcc atgttgaccg ttcaattaca ggtcctgcgg catttattga
     2641 aaccaatatt gttggtactt atgtcctttt ggaagccgct cgcaattact ggtctgctct
     2701 tgatagcgac aagaaaaata gcttccgttt tcatcatatt tctactgacg aagtctatgg
     2761 tgatttgcct catccagatg aagtaaataa taacgaagaa ttacccttat ttactgagac
     2821 gacagcttac gcaccaagca gcccttattc cgcatcaaaa gcatccagcg atcatttagt
     2881 ccgcgcgtgg aaacgtacct atggtttacc gaccattgtg actaactgtt cgaataacta
     2941 cggaccttat cactttccgg aaaaattgat tccactagta attcttaatg ctctggaagg
     3001 taaggcatta cctatttatg gcaaagggga tcaaattcgt gactggctgt atgttgaaga
     3061 tcatgcgcgt gcgttatata ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat
     3121 tggtggacac aacgaaaaga aaaacatcga tgtagtgctc actatttgtg atttgttgga
     3181 tgagattgta ccgaaagaga aatcttatcg tgagcaaatt acttatgttg ccgaccgccc
     3241 gggacatgat cgccgttatg cgattgatgc tgagaagatt agccgcgaat tgggttggaa
     3301 accacaggaa acgtttgaga gcgggattcg taaaacagtg gaatggtacc tgtccaatac
     3361 aaaatgggtt gataatgtga aaagtggtgc ctatcaatcg tggattgaac agaactatga
     3421 gggccgccag taatgaatat cctccttttc ggcaaaacag ggcaggtagg ttgggaacta
     3481 cagcgtgctc tggcacctct gggtaatttg attgctcttg atgttcactc cactgattac
     3541 tgtggtgatt ttagtaatcc tgaaggtgta gctgaaaccg taagaagcat tcggcctgat
     3601 attattgtca acgcagcagc tcacaccgca gtagacaaag cagaatcaga accggagttt
     3661 gcacaattac ttaacgcgac gagtgtcgaa gcgatcgcga aagcagccaa tgaagtcggc
     3721 gcatgggtta ttcactactc tactgactac gtatttccgg ggaccggtga aataccatgg
     3781 caggaggcgg atgcaaccgc accgctaaat gtttacggtg aaaccaagtt agctggagaa
     3841 aaagcattac aggaacattg cgcgaagcat cttattttcc gtaccagctg ggtatacgca
     3901 ggtaaaggaa ataatttcgc caaaacaatg ttgcgtctgg caaaagagcg tgaagaatta
     3961 gccgttatta atgatcagtt tggtgcgcca actggcgcag agttgctggc tgattgtacg
     4021 gcacatgcca ttcgtgtagc actgaataaa ccggaagtcg caggcttgta ccatttggta
     4081 gccagtggta ccacaacctg gcacgattat gctgcgctgg tttttgaaga ggcgcgcaaa
     4141 gcaggcattg cccttgcact caacaagctc aacgcagtac caacaactgc ctatcctaca
     4201 ccagctcgtc gtccacataa ctctcgcctt aatacagaaa aatttcagca gaactttgcg
     4261 cttgtcttgc ctgactggca ggttggcgtg aaacgaatgc ttaacgaatt atttacgact
     4321 acagcaattt aatagttttt gcatcttgtt cgtgatggtg gagcaagatg aattaaaagg
     4381 aatgatgaaa tgaaaacgcg taaaggtatt attttagcgg gtggttctgg tacacgtctt
     4441 tatcctgtga ctatggctgt cagtaaacag ctattaccta tttatgataa accgatgatc
     4501 tattacccgc tctctacact gatgttagcg ggtattcgtg atattctgat tattagtacg
     4561 ccacaggata ctcctcgttt tcaacaactg ttgggtgatg gtagccagtg ggggctaaat
     4621 cttcagtaca aagtgcaacc gagtccagat ggtcttgcgc aggcatttat catcggtgaa
     4681 gagtttattg gtggtgatga ttgtgctttg gttctaggtg ataacatctt ttacggtcac
     4741 gatctgccga agttaatgga cgcagctgtt aacaaagaaa gtggtgcaac ggtatttgcc
     4801 tatcacgtta atgatcctga acgctacggt gtcgttgagt ttgataaaaa cggtacggca
     4861 ataagcctgg aagaaaaacc gttacaacca aaaagtaatt atgcggtaac cgggctttat
     4921 ttctatgaca acgacgttgt cgaaatggcg aaaaacctta agccttcttc ccgtggtgaa
     4981 ctagaaatta ccgatattaa ccgtatttat atggaacagg ggcgtttatc tgttgccatg
     5041 atgggccgtg gttatgcatg gctggacacg gggacacatc aaagcctgat tgaggcaagc
     5101 aacttcattg ctacaattga agagcgtcag gggttaaaag tttcctgtcc ggaagaaatt
     5161 gcttaccgta aagggtttat tgatgctgag caggtgaaag tattagctga accgttgaag
     5221 aaaaatgctt atggtcagta cctgctaaaa atgattaaag gttattaata aaatgaacgt
     5281 aattaaaaca gagattccag acgtattaat tttcgaaccg aaagtttttg gtgatgagcg
     5341 tggtttcttt tttgagagct ttaaccagaa ggtttttgag gaagctgtag gccgcaaagt
     5401 tgaatttgtt caggataacc attcgaagtc tagtaaaggt gtactccgag ggcttcactt
     5461 tcaacgtccg ccttttgagc aagcaaagtt ggtaagatgt atcgttggtg aagtttttga
     5521 tgtcgcagtg gacatcagag ttggttctga ttcatatggt cattgggttg gtataatcct
     5581 ttcctcggaa aataaacggc aattgtggat accagaagga tttgcgcatg gttttctggt
     5641 tctaagtgac gaagctgagt ttgtatataa aactactaat tattattgtc ctgaatttga
     5701 agcgggaata agttggtgtg atggtttttt gaacataaat tggcctcagt tagatgaaat
     5761 taaactttca ttgaaagata agcagtgggg tgggataaat gcatttaaaa gctaacgcga
     5821 gtctgattaa tgcttaaaaa tatattcttt ctttttttga ttcagatttc taattatatt
     5881 tttcctttgc ttacactgcc atatttagta agggtcttag gagcttctca attcggttct
     5941 ttaatgttag cacaagcatt aatacagtac tttattactg taacagagta tggttttaat
     6001 cttagtgcca caaaaaaaat agccttgtct aaatctcagg atgagactaa taaaatatat
     6061 acaaatacat tatatgctag aataatattg gcgttgatat gttttattat agctatttta
     6121 tgtacgcaat ttattgaaat gtttgagcgt gaatttaatg ttataataat actattcctt
     6181 ggtgtaattg gtaattgttt tttccctatt tatcttttcc aaggactgga gttaatgaaa
     6241 aaaatagctt ggataagtat tgtttcaaaa acatttatga ccttaagtgt atttgtgtta
     6301 gtcaaagatg caaaagatat aaataatgct gcattagcgt tagcaatacc tttattactc
     6361 cccggaatta tttcttttat ttatatttac aatagacagt tagcaagagt agtttcgttt
     6421 agctttaggg gtattgtaaa agagttgaag gatggagggg ccctgtttat ttcacaaata
     6481 gctattagtt tttatacgac atttaatact ttaattctgg ggtatatttt taacccaact
     6541 atcgtaggcg tttttagcgc agctgataaa ctaagaatag ctgcgcaatc atgttacgtg
     6601 ccaattcaac agrtagtttt cccaagaata aataaggaag caggagaact ttcaacaaag
     6661 ttgttaaagt atgggggatt ttttgtatta atatcattct tgggatcttc attggttttc
     6721 ttttttggag aacaattagc actattatat ctaggtaatg aatttattat atcggcaaga
     6781 ctctttaaat ggatgtcact tttgttgttt attattagta ttgcaatagt atttggtcaa
     6841 tggggattaa taacattagg gaaagaaaaa ttacttacaa aaatttatgt tataggtgca
     6901 ctatgtcatg ttggctactc tattccttta gttatgggct acaatgttta tggcatgtta
     6961 gtttcagttc ttattactga atttatcatc acactaatga tgatatttag tttttataag
     7021 gttactcatt cggcgatatg agatagaaat ggtatatata attgtattaa attggaataa
     7081 ttctgaggac acaatttctt gtatttcgac actgctaaaa caaaactata tatattttag
     7141 aattgtttta gtggataatg attctactga tgattcatta ataaaaatat tggcattcct
     7201 aaagaaatcg tgccaaaact atttgcactt aagtgaatct gaatcatatt tagatgagga
     7261 aattcataca aataaaataa cggtcatttc tgcaactagt aataaaggtt atgccggcgg
     7321 aaataatatt ggcattaagt atgcgcttca gtcaaatgat tgtaaatata tatgggtatt
     7381 aaataatgac acagaagttg aatcgaattc attatcactg ttagtgagtg aaatggaacg
     7441 agataaagaa attggaattt tagggagcaa gttagtttat tatcataata aaaatgtcat
     7501 tcaaggctta ggggggaaat atcttccatg gcgcttttta acacagcata ttgcctcgaa
     7561 tcatttaata acttctaagt atgataacga tactatctct aagcaaatag attatattat
     7621 aggtgctgct ttatttgtta aggctgaggt atttaagact gtagggctct tagatgaaca
     7681 atatttcctt tatttcgaag agctagatat ttgtttgcga gcaagaaaac ataactataa
     7741 actaaaaaca attactgaca gcatagtata tcataaagaa ggcgcaacaa tatctaaaga
     7801 aaaaaatgcc ttctcagaat atcactatct tcgtagtaac aagttgttta tacgcaaatt
     7861 ttatccacaa acattgtttt tatataatat gatgttaata gctaaaattg caaatagagt
     7921 tcgtagaggt cagtttgatc tcgcaaaagc taacttaaaa gtccttcttg gaataaaata
     7981 tgttaactca aaaacatgag atgaagattg ataacatggg aaataatatt aagaaagttc
     8041 tttttttcaa tccgttaaag aaaattatga tccgtgggat ttcagtatac tctggtgaat
     8101 atgagcaact attaagagat aaaaacgttg aagttatagt gtatgaatta ccattatggt
     8161 ttggtaaaat gccaaaggct atgcaagtat ttggttttat gtttgcccaa caagtgctat
     8221 tgcctattcg ggctttaaag gttaagccag accttatttt tgatgcttat aatacttatt
     8281 ctatattagc agcgttaaaa tttaattata ttttcgtaat tcacgacttt atacctttta
     8341 ataataaact ttggttttta aaaccaggtg ctttatatca aaaaatatta cacaatttgt
     8401 ctaaaaactt ttccaagttg cacctgtgtt atataaacag tagaattata aatgataaaa
     8461 atgcaattat caaaaaaaaa tattttatat taccaaatat cgttagatca ttgggatatc
     8521 ataaggtttc taatgtagag attgaaaact tcatcaaggc agggagagag cttgatgcga
     8581 tatgtatcgt aactatctcg ggttccggta aaaataaaga ctttggtggt ttagttgaaa
     8641 gattaaataa ccttaacgtt cctgtatatt tagttgcttt tggttttgcc aataattttt
     8701 gtgaagaatt taataatgtt aatgtttttt caccaggagt tgtggattcg aatttaattg
     8761 gttcggtaat aacaaatagc gatttattcg tgtttcactc aactagtgaa ggttttggga
     8821 gaccaattat tgaagcactt gaattaaagg ctaaagtaat atgttcagat gtttctcctt
     8881 cccttgatta tattaatgaa gagtatctgc ctaatctcta tatctatagt ttaagagaca
     8941 tgaatgaatt caagtatcaa tttgataaag cactacggac gccaaaaagg ttaattcaaa
     9001 gtgactcagt tttgacttat aatgacgagg atttattcaa tgaaatatct cgaataattg
     9061 aggcctgaaa tgttgtattt aactaatctg atatttcttt atcaaataat attgacagta
     9121 ttactttttc tccctagttc tattttttca ggattaatta tagagtacac aataccctta
     9181 ttattcattt ttgttatgtc agcgacaata tacttcatca caaagagagg gaactcattt
     9241 aaatattgtc aaatttctat atgcttacct tcaaagctta tacttcaatg tttctttttt
     9301 ttctttattg cattaaactt ttctgaattt atctttgtag ctactcatgg tggtcatcaa
     9361 gctttgtata tggctgatcg acctttgttt ccttttttga tctttaagct attagcattc
     9421 cctgcttttt atttcgcgac aattattatt cttactaaaa attatgtggt ttcaagattg
     9481 ttagtttggc ttgctctatt cagtcctctg ttgacaggta gtagaggaat gaccattttt
     9541 ttattattgg cctatctatt tgttactttt gggccatttt tctttatcag attcaaattt
     9601 gccattcttg gttttttaat gattattagc tttattggta taggttatta tcgtgaaccg
     9661 attaatatga gtatagcgga ttatataata ttggttgtat catctttaaa tgagttcgca
     9721 tttgcgtcta tgaatatttc taattgccaa atcccttttt caaatgtgtt tactcaattc
     9781 ggtgatatat tcttaggagc aaaaagtgac tttcgagttg ctactcttct gaccgaatgt
     9841 gtttcccctg gcgctagtga gaaaggttat ggcatttcat ccagcattgt aggcgagtct
     9901 aagatacttg gtgacaattt atggtttctt tactttattg ctatcttgtc tataaattca
     9961 attatgatat cgattttgtt gcgatctggc tcacgtcttt cgcatatggt tggcatttca
    10021 ttattaccat ttatcctcta ttcagtacgc acagagattg tttatcctta tgttttttta
    10081 ataaaaatcc ttatttatat cttaatagct atgataatta atcagatttt agtaaaaagc
    10141 agtgctgtgg aaggtgcgaa caagttcctg atatgagatc atcatattca tccggagcgc
    10201 atcccagagg gacatcatga gccatcaact caccttcgcc gatagtgaat tcagcactaa
    10261 gcgccgtcag acccgaaaag agattttcct ctcccgcatg gagcagattc tgccatggca
    10321 aaacatggtg gaagtcatcg agccgtttta tcccaaggcg ggcaatggcc gacggcccta
    10381 tccgctggag accatgctgc gtattcactg catgcagcat tggtacaacc tgagcgacgg
    10441 tgccatggaa gatgccctgt acgaaatcgc ctccatgcgc ctgtttgccc gattatccct
    10501 ggatagcgcc ctgccggatc gcaccaccat catgaatttc cgccacctgc tcgagcagca
    10561 tcaactggcc cgtcaattgt tcaagaccat caatcgctgg ctggccgaag caggcgtcat
    10621 gatgacccaa ggcactttgg tggatgccac catcattgag gcacccagct ctaccaagaa
    10681 caaagagcag caacgcgatc cggagatgca tcagaccaag aaaggcaatc agtggcactt
    10741 tggcatgaag gcccacattg gtgtcgatgc caagagtggc ctgacccaca gcctagtcac
    10801 caccgcggcc aacgagcatg acctcaatca gctgggtaat ctgcttcatg gagaggagca
    10861 atttgtctca gccgatgccg gctaccaagg agcgccacag cgcgaggagc tggccgaggt
    10921 ggatgtggac tggctgatcg ccgagcgtcc cggcaaggta aaaaccttga agcagcatcc
    10981 gcgcaagaac aaaacggcca tcaacatcga atacatgaaa gccagcatcc gtgccagggt
    11041 ggagcacccg tttcgcatca tcaagcggca gttcggcttc gtgaaagcca gatacaaggg
    11101 gctgctgaaa aacgataacc aactggcgat gttattcacc ctggccaacc tgtttcgggt
    11161 ggaccaaatg atacgtcagt gggagagatc tcagtaaaaa ccggaaataa cgccagaaat
    11221 ggtggaaaaa atagcctaaa taggctgatt cgatgtgttt gcgggaaaaa aatcggccca
    11281 gatccgagaa attttaatca gcgagtcagc ttgggaagaa atgacctgct tattcgcacc
    11341 ttccctagtg cagttgtggc gtggcaagat gacaatcatc atttccgtca ttgtggctat
    11401 tgccctggct attggatatt tggcagtagc gaaggagaaa tggacgtcaa cagcaattat
    11461 cactcagccc gacgtggggc aaattgctgg ctataacaat gccatgaatg ttatctatgg
    11521 tcaggctgcg ccgaaagtat cggatttaca ggaaacgttg attggtcgct tcagttctgc
    11581 cttctctgca ttagcagaaa cgctggataa tcaggaagaa ccagaaaaac ttaccatcga
    11641 accttctgtt aagaaccagc aattaccatt gactgtttct tatgttgggc aaactgcaga
    11701 gggagcacaa atgaagttgg cccaatacat tcagcaagtt gatgataaag tgaatcaaga
    11761 gctagaaaag gatctcaagg acaacattgc tctgggacgg aaaaacttgc aggactcttt
    11821 aagaacgcag gaagtagtcg cgcaggagca gaaagatctg cgtatccgtc agattcagga
    11881 agcgttgcag tatgcgaatc aggcgcaggt gacaaagcca caggttcagc agactgaaga
    11941 tgtgacgcaa gatacgttgt tccttttggg gagcgaagcg ctggagtcta tgattaagca
    12001 tgaggcgacc cgtccgttgg tgttctcacc aaactactat cagactcgtc aaaacctgct
    12061 ggatattgaa aacttaaggg ttgatgatct tgatattcat gcttaccgct atgtgatgaa
    12121 accgacgtta cctattcgtc gcgatagccc gaaaaaggca attaccttga ttctggcagt
    12181 gctgctgggt ggcatggttg gcgcggggat tgtgctgggg cgtaacgctc tgcgtaatta
    12241 caacgcgaag taatctttgc agttttaaag aaaaagggca gggtggtgac accttgcccg
    12301 tttttttttg ccggatgcga caacaatatc gcatccgctt accccgcaac tcactgatgc
    12361 cgtttacgca ggttctcaat taccgtcgtt aaatccagat cctgatcctg caacagcacc
    12421 agcaggtgat acatcaaatc agacgcctcg ttggtgagct caaagcggtc atgtaccgtc
    12481 gctgccagtg cggtttccac cccttcttcg cccactttct gcgcaatgcg tttggtgccg
    12541 ctggcataca gtttggcggt ataggaggtt tccgggtcgg cagatttgcg ttcggcgagc
    12601 agttgttcca gttgatacag gaacagccac tggtgagcgg tgttgccgaa gcagctgctg
    12661 gtgcctttgt ggcaggttgg tccgatagga ttcgccagta ccagtagcgt gtcgttgtcg
    12721 cagtccgggg caatactcac cacattgagg aaattgccag acgtttcgcc tttggtccat
    12781 agtcgctgtt tagtgcgcga gaagaaggtg actttgccgc tttcgatggt tttgtctacg
    12841 gcttccgggt tcatgtagcc cagcattaac acttcaccgg ataccgcgtg ttgcacaatc
    12901 accggcatca gtccgtcggt tttttcccag tccagttcgc gacattgttg ttctgttaac
    12961 atatcctgat ctccacgccc tgtgttgcca ggtacgcttt taattcacca atattgatta
    13021 tttgtttgtg gaatacggaa gccgccagcg cgccgtcaac gtcggcatcg cggaaggctt
    13081 cgaggaagtg ttccatggtg cccgcgccac cggaggcaat cagcgggacg tggcaaactt
    13141 cacgcacttt tttcagctgt tcgaggtcgt aaccgttacg cacgccgtcc tgattcatca
    13201 tgttgaggac gatttctccg gcaccgcgtt tttgcacttc ctgtacccag tcgagtgttt
    13261 cccactgagt aacgcgggtg cggctttcat cgcc
//
