LOCUS       GU445927               13743 bp    DNA     linear   BCT 20-JUN-2010
DEFINITION  Escherichia coli serogroup O71 O-antigen gene cluster, complete
            sequence.
ACCESSION   GU445927
VERSION     GU445927.1  GI:298353045
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13743)
  AUTHORS   Hu,B., Perepelov,A.V., Liu,B., Shevelev,S.D., Guo,D.,
            Senchenkova,S.N., Shashkov,A.S., Feng,L., Knirel,Y.A. and Wang,L.
  TITLE     Structural and genetic evidence for the close relationship between
            Escherichia coli O71 and Salmonella enterica O28 O-antigens
  JOURNAL   FEMS Immunol. Med. Microbiol. 59 (2), 161-169 (2010)
   PUBMED   20482625
REFERENCE   2  (bases 1 to 13743)
  AUTHORS   Hu,B., Perepelov,A.V., Liu,B., Shevelev,S.D., Guo,D.,
            Senchenkova,S.N., Shashkov,A.S., Feng,L., Knirel,Y.A. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2010) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P. R. China
FEATURES             Location/Qualifiers
     source          1..13743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O71"
     gene            1137..2222
                     /gene="rmlB"
     CDS             1137..2222
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ADI77024.1"
                     /db_xref="GI:298353046"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGGTYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKTGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVVNVKSGAYQSWIEQNYEGRQ"
     misc_feature    1137..12248
                     /note="O-antigen gene cluster"
     gene            2222..3121
                     /gene="rmlD"
     CDS             2222..3121
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ADI77025.1"
                     /db_xref="GI:298353047"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKLSQNPNLHNYSMRPALNQLQKRPMKVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQENCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAISTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3179..4051
                     /gene="rmlA"
     CDS             3179..4051
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ADI77026.1"
                     /db_xref="GI:298353048"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFEQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEDFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKKLKPSARGEVEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIGKEQLELLAKSL
                     MKNQYGKYLLKMIG"
     gene            4078..4887
                     /gene="wdaK"
     CDS             4078..4887
                     /gene="wdaK"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WdaK"
                     /protein_id="ADI77027.1"
                     /db_xref="GI:298353049"
                     /translation="MNFSVLMSLYIKEKPEYLHECLKSLYEQTKQADEIVLVYDGPIT
                     NELDKVVNYWLNSLPIKIIKLSKNVGLGQALNKGLAQCENDYVARMDTDDICHPKRFE
                     IQIDFLNKNKNISVVGSYIEEFSETIENRLSQKIVPTTHNEIMKNIGKRNPFNHMTVV
                     FNKSEIIKVGGYEHHYLMEDYNLWLRLLKNNIQAANLPLTLVYARGGDGMIYRRSGLR
                     YIKSEYKLYKLKQRLGYQNYIISSLVFFLRSVPRVFPPYILKAIYRFLRKK"
     gene            4925..5473
                     /gene="rmlC"
     CDS             4925..5473
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ADI77028.1"
                     /db_xref="GI:298353050"
                     /translation="MKYSRTTIQDAIVITPEVFADSRGYFFESFNYRDFQRIINREIV
                     FCQDNQSLSHKGVVRGLHFQVNPKEQAKLVRCINGSVYDVIVDLRKNSPSYKCWFGIL
                     LSSDNKKQLWIPEGCAHGFISLEDNTELLYKTTEFYSPDHERCIIWNDPDLGISWPEY
                     SYIISDKDKNGISFAEWESLGD"
     gene            5466..5864
                     /gene="qdtA"
     CDS             5466..5864
                     /gene="qdtA"
                     /note="dTDP-4-oxo-6-deoxy-D-glucose-3,4-oxoisomerase"
                     /codon_start=1
                     /transl_table=11
                     /product="QdtA"
                     /protein_id="ADI77029.1"
                     /db_xref="GI:298353051"
                     /translation="MIKVKLIPLQTHGDDRGSLVALEEGYNIPFQIKRVYYIFNTKKG
                     VMRGFHAHKNLKQVAIAVRGSCKFKLDNGTDSVEVLLNDPAQGLLIESFIWREMYDFS
                     EDCVLMVLADSHYDENDYVRDYNAFLNLVA"
     gene            5884..6990
                     /gene="qdtB"
     CDS             5884..6990
                     /gene="qdtB"
                     /note="transaminase"
                     /codon_start=1
                     /transl_table=11
                     /product="QdtB"
                     /protein_id="ADI77030.1"
                     /db_xref="GI:298353052"
                     /translation="MVNFLSLKNINSRYNEELKKACARVIDSGWYVMGEELHQFENEF
                     AKYCGVKGSLGVANGLDALILSLRAWREMGKIKPGDEVLVPANTYIASIIAITENNLV
                     PVFVEPDYNTYNLDPDKIESAITEKTKVILPVHLYGRICPMEEILSLAKKYNLLVLED
                     CAQAHGASLNGRKTGGWGDAGAFSFYPGKNLGALGDSGAITSNDLDFLNVIQALRNYG
                     SHQKYVNQYIGVNSRLDEIQAAMLRVKLKYLDSDNKRRKEIAQYYIDSIINPLVQLPK
                     SCSNYDENVWHLFVVKTRYREYLQKFLSKQSIQTLIHYPHPPHKQIAYKQFNSISLPL
                     TEKIHNEVLSLPMDPTLSEEEIQKVVNAVNGFTL"
     gene            6987..8261
                     /gene="wzx"
     CDS             6987..8261
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADI77031.1"
                     /db_xref="GI:298353053"
                     /translation="MSSFFKISFFSGCLTLFKMLMGFLIAKVIAIYTGPSGMALLGQL
                     QSLVTGINGIVNAPVGNGIVKYTAEFKLEGAECCSRWWKPAIGFSFLFCLVLSIVFIP
                     FANKISFILFGSYEYDYVVIIAVCNLPFAAFGTMIMSIVNGMGKYRLYILYGFISNLI
                     TSIIMLLMLIKYGIVGALLATSIQYGLIGIILICLNYNQEWFKHNYFYPYDSIFNSEA
                     KDILAYILMAITSAISLPLTLIVIRKILLNMTDITTVGQWQAVWKISEVYIGVVTIAL
                     STYFLPKLSALNDTAKIKNEIGIVLKYTIPFVCVAAVLIFAFRDLIITLLFTNEFYPA
                     RDLFFIQLVGDIIKIISWIYAFTMLASKATRWYVGSELFFCISWCLISFVLVNIYKAQ
                     GINIAYCISYVMYFLIIYLNLNKIIKKKNEPH"
     gene            8248..9666
                     /gene="wdaN"
     CDS             8248..9666
                     /gene="wdaN"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WdaN"
                     /protein_id="ADI77032.1"
                     /db_xref="GI:298353054"
                     /translation="MNLIEIIKLQIIKTSDINKKIRLLKELGGMLWANNVGIYNLNSL
                     ERAVIEDVARDYTPQIGSQYKKKKNNVFVITIGYMSGGHTRLMENLANMLDNKCDLII
                     TGNASVTLKKRLEKYFNEIFEIPVYNDTNINELYKLSANLERYENIILNIHPDDIASV
                     IAAGLAKKNINTYVHFVNHADHVFSFGPTVADVWYQISQFGAKLDQLRELSGKTTFLG
                     IPIAINDDHLSNNMISTNDEVKCIITAGSANKYKPHKGYSLIKKIPEILYQFPEASII
                     VVGTNILFCYWWWGTFLKFRKRIKFYKALNHSEYLLLMQNADIYLDSYPIPGGTAFVE
                     QSFLGKKCAGYISPQQGYTPLENIKCDITSSELNKINNVDIIEKIKYVHPFDNVKNRL
                     LSAINKQVMTTINWDYYYKWTGDINFFKLEKIREIPLYLLLECVKENSSLKCIISQYK
                     FITIISLIKNFIRLYVSKLKGK"
     gene            9666..10955
                     /gene="wzy"
     CDS             9666..10955
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADI77033.1"
                     /db_xref="GI:298353055"
                     /translation="MEYTIIIIFFVLLYFTSKVTWPFSGKNAHPINFVLFTQIFILTL
                     PGVLILTFFKECAGSITCEIIAENIKINVMLDYFTVLCFILLGVLSSFFLLKGKTDFR
                     PASNAHRRYLNTCFILTLLIFLVKIISVNQVPLFLTLLGHKDAAEIQKAEILRGEDGF
                     SFPVINFLIKYFPLYFYYSTLIGLFKRKVTIVYFVMAFLVTSVTMLYDLQKGPVVIMI
                     IGSFWLYWAYTGKAKMIVVGGIFSLFLVGVLFYISFDFGDDTQYFITAVLNRTFIAQN
                     DGMYWVYQYYNILPNKELYSLWGVPLAQQFGIPQIDPLSDIIGVVFPQAKDSWVNTTT
                     FLLGEAKAIFGDYSSIISSLVVFINVFVVCVLSTLLVRVSKNIFYPAIFVMIQTIPFA
                     NNLTDLLYGRFIFGFLVFMLFPVLICFLCYGKLKNDE"
     gene            10945..11775
                     /gene="wdaM"
     CDS             10945..11775
                     /gene="wdaM"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WdaM"
                     /protein_id="ADI77034.1"
                     /db_xref="GI:298353056"
                     /translation="MMNRKVLIIMVTYNPDWSKVINKVKSFCVAGTVFVSDNSDSDSS
                     SLINYENVIYRYNHGNVGIAKAQNLGIEYAMQYNYEFVAFVDDDSNLSGGKITQLTNN
                     LCKLGSLGENIAAFCAYPSERGGFDKTKKTSLGRQLLLTKNLMSSGSLTKVDVFKKIG
                     LFDEDLFIDYVDYEWGWRALEKNFLIVIDTSVEFEHSLGQGRSKIGLGIPSPIRHFYQ
                     TRNLLWISRLNYVPIYWKLKQFLLLPIRYFYFGFFYNHSKLRRTHFLKGFAAGLKFKS
                     "
     gene            complement(11778..12248)
                     /gene="qdtC"
     CDS             complement(11778..12248)
                     /gene="qdtC"
                     /note="transacetylase"
                     /codon_start=1
                     /transl_table=11
                     /product="QdtC"
                     /protein_id="ADI77035.1"
                     /db_xref="GI:298353057"
                     /translation="MKIHPLSDVKTVKIGLNTTIWQYSIIFEGAVIGNNCNICAHTLI
                     ENKVIIGDNVTIKSGVYIWDGVIIEDNVFIGPNVTFTNDIYPRSKKYPDYYPTIHIKK
                     NASIGANATILPGIIIGENSIIGAGSVVTKDIPDNVIVAGNPAKIIRPVDDIIS"
BASE COUNT     4244 a   2296 c   2889 g   4314 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc atatgaatta gaatctctcc ttgagcagcg cgtgaagcgt
      121 caactgcttg cggaagtgca gtccatctgt ccaccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt aggccactcc attttgtgtg cacgacccgc cattggtgac
      241 aacccatttg tcgtggtgct gccagacgtt gtgatcgacg atgccagcgc cgacccgcta
      301 cgctacaacc ttgctgccat gattgcgcgc ttcaatgaaa cgggccgtag ccaagtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca tccagaccaa agaaccgctg
      421 gaccgtgaag gcaaagtcag ccgcattgtt gaattcatcg aaaaaccgga tcagccacac
      481 acgctggact cagacatcat ggccgtgggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcagcc tggtgcatgg gggcgtattc agctgactga tgccattgcc
      601 gaactggcga aaaaacagtc cgttgatgcc atgctgatga caggtgacag ctacgactgc
      661 ggtaaaaaaa tggggtatat gcaggcattt gtgaagtatg gactacgcaa cctcaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ctgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcgaa agtaatttgt
      841 tgcgaatatt cctgccgtta ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggcaacgc tcgtcacatc gtagacatgc
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctcttaa
     1081 tcaaactaag agccgctaat ttcacagcat gctctgaagt aatatggaat aaaaaagtga
     1141 agatacttgt tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa
     1201 ataatacgca ggatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctggaat
     1261 cacttgctga tgtttctgat tctgaacgct atgtttttga acatgcggat atttgcgatg
     1321 ctgctgcaat ggcgcggatt tttgctcagc atcagccgga tgcagtgatg cacctggctg
     1381 ctgaaagcca tgttgaccgt tcaattacag gtcctgcggc atttattgaa accaatattg
     1441 ttggtactta tgtccttttg gaagccgctc gcaattactg gtctgctctt gatagcgaca
     1501 agaaaaatag cttccgtttt catcatattt ctactgacga agtctatggc gatctgcctc
     1561 atcctgacga agtaaataat aaagaagaat tacccctctt tactgagacg acagcttacg
     1621 cgccaagcag cccatattct gcttctaaag catccagcga tcatttagtc cgcgcgtgga
     1681 aacgtaccta tggtttaccg accattgtga ctaactgttc gaataactac ggcccttatc
     1741 actttccgga aaaattgatt ccgttggtaa ttcttaatgc tctggaaggt aaggcattac
     1801 ctatttatgg caaaggggat caaattcgtg actggctgta tgttgaagat catgcgcgtg
     1861 cgttatatac cgtcgtaacc gaaggtaaag cgggtggaac ttataacatt ggtggacaca
     1921 acgaaaagaa aaacatcgat gtagtgctca ctatttgtga tttgttggat gagattgtac
     1981 cgaaagagaa atcttaccgc gagcaaatca cttatgttgc cgatcgtccg ggacacgatc
     2041 gccgttatgc cattgatgct gagaagactg gtcgcgaatt gggatggaaa ccacaggaaa
     2101 cgtttgagag cgggattcgg aagacagtgg aatggtacct gtccaataca aaatgggttg
     2161 ttaatgtgaa aagtggtgcc tatcaatcgt ggattgaaca gaactatgag ggccgccagt
     2221 aatgaatatt ctccttttcg gcaaaacagg gcaggtaggt tgggaactac agcgtgctct
     2281 ggcacctttg ggtaatttga ttgctcttga tgttcactcc actgattatt gtggtgattt
     2341 tagtaatcct gaaggtgtgg ctgaaaccgt caaaaaaatt cgccctgatg ttattgttaa
     2401 tgctgcggct catactgcag tagataagct gagtcagaac ccgaatttgc acaattactc
     2461 aatgcgacca gcgttgaatc aattgcaaaa gcggccaatg aaagttgggg cttgggtaat
     2521 tcattactca actgactacg tatttccggg aaccggtgaa ataccatggc tggagacgga
     2581 tgcaaccgca ccgctaaatg tttacggtga aaccaagtta gccggagaaa aagcgttaca
     2641 ggaaaattgc gcgaagcatc ttattttccg taccagctgg gtatacgcag gtaaaggaaa
     2701 taacttcgcc aaaacgatgt tgcgtctggc aaaagagcgc gaagaactgg ctgtgattaa
     2761 tgatcaattt ggtgcgccaa caggtgctga gctgctggca gattgtacgg cacatgctat
     2821 tcgtgtggca ctgaataaac cagaagtcgc aggcttgtat catctggtag ccagtggtac
     2881 cacaacttgg cacgattatg ctgcgctggt ttttgaagag gcgcgcaaag caggcattcc
     2941 ccttgcactc aacaagctca acgcaatatc aacaacagcc tatcctacac cagctcgtcg
     3001 cccacataac tctcgcctta atacagaaaa atttcagcag aactttgcgc ttgtcttgcc
     3061 tgactggcag gttggcgtga aacgaatgct caacgaatta tttacgacta cagcaattta
     3121 attgtttttg catcttgttc gtgatgatgg tgcaagatga attaaaagga atgatgaaat
     3181 gaaaacgcgt aaaggtatta ttttagcggg tggttctggt actcgtcttt atcctgtgac
     3241 tatggccgtg agtaaacaac ttctgcctat ttatgataag cctatgattt attacccgct
     3301 ctcaaccctg atgctggcgg gtattcgtga tattttgatt attagtacgc cacaagacac
     3361 accacgtttt gagcaattac tgggcgacgg tagccagtgg gggcttaatc ttcagtacaa
     3421 agtgcaacct agtccagatg gtctggcaca agctttcatt attggagaag attttattgg
     3481 tggtgatgat tgtgcgttgg tcttgggtga taatatcttt tacggtcatg acctaccaaa
     3541 attaatggat acagctgtta atagagagag tggcgcaacg gtatttgcct atcacgttaa
     3601 tgatccagaa cgttatggtg tggtggagtt tgataaaaac ggcacggcaa taagcttgga
     3661 agaaaaaccg cttcaaccga aaagcaatta tgcagttaca ggactttatt tctatgataa
     3721 tgatgtcgtg gaaatggcaa aaaaactgaa accatctgcc cgtggcgaag tcgaaatcac
     3781 agatattaac cgtatttata tggaacaggg gcgtttgtct gtagccatga tggggcgtgg
     3841 ttatgcttgg ttggacacgg gtacacatca aagtcttatt gaggcaagta acttcattgc
     3901 aacaatagaa gaacgccagg ggctgaaagt ttcctgtcca gaagagattg cttaccgtaa
     3961 agggtttatt ggaaaagagc aactagagct attagctaag tcgcttatga aaaatcaata
     4021 tggaaaatat cttcttaaaa tgatcgggta aataataata ctttttcagg aaatgaaatg
     4081 aatttcagtg tattgatgtc tctttatatt aaagagaagc ccgaatatct acatgaatgc
     4141 ttaaaaagtc tatatgaaca aacaaagcaa gctgatgaga tagtgcttgt ctatgatgga
     4201 ccaattacaa acgaattaga taaagtagtt aattattggt taaatagttt gccaataaag
     4261 ataataaaat taagcaaaaa tgtcggtttg ggtcaggccc tgaataaggg gttagctcaa
     4321 tgtgaaaatg attatgtcgc tagaatggat acggatgata tatgccatcc caagagattt
     4381 gaaattcaaa ttgatttttt aaataaaaac aagaatataa gtgttgttgg ttcatatata
     4441 gaggagtttt cagaaactat agaaaacagg ttgtcgcaaa aaattgtacc gacaacgcac
     4501 aatgagataa tgaaaaatat aggaaaacga aatccattta accacatgac agttgtgttc
     4561 aataagagtg aaataataaa ggtgggtgga tatgaacatc attatttaat ggaagattat
     4621 aatttatggt tgcggctttt aaaaaataat attcaggcag ccaatttgcc tttaacatta
     4681 gtttatgcca gaggtgggga tggcatgatt tataggaggt ccggtttacg ttatattaaa
     4741 agtgaatata aattgtataa gttaaaacaa agattaggat atcaaaatta tattatatcc
     4801 tcattggtgt tttttttaag gagtgtacca agggtttttc ctccttacat tttaaaagca
     4861 atttatagat tcttgcgaaa gaaataacag tataaaaatg cattatatta tttagagggg
     4921 gcttatgaaa tattcaagaa caacaataca agatgctata gtgattacac ctgaagtatt
     4981 tgctgattct agggggtatt tttttgaaag ctttaattat cgggattttc aaaggattat
     5041 taatcgagag atagtttttt gtcaagataa ccaatcatta tcgcataagg gagttgtcag
     5101 gggactacat tttcaagtaa atccaaagga acaggcaaaa ttggtaagat gtataaatgg
     5161 ttcggtgtat gatgtgattg tagacttgcg caaaaactcc ccttcttata agtgttggtt
     5221 cggaatttta ttatcgtctg ataataaaaa acagttatgg attccagagg gatgtgctca
     5281 tggttttatc tcattagagg acaatacgga attattatat aagactactg aattttattc
     5341 tcctgaccat gaacgctgca tcatttggaa tgatcctgac ttaggaataa gttggccaga
     5401 atatagctat ataatttccg ataaagataa gaatggaatt tccttcgctg aatgggagag
     5461 cttaggtgat taaggtgaaa ttaattccac ttcaaacgca tggtgacgat agaggttcgc
     5521 ttgttgcttt agaagagggg tataatatac catttcaaat taaacgcgtt tattatattt
     5581 ttaacacaaa gaaaggagtc atgagaggtt ttcatgcaca taaaaactta aaacaggttg
     5641 caatagctgt tagaggaagt tgtaagttta aattagacaa tggtaccgat agtgttgagg
     5701 ttctactgaa tgatcctgcg caaggtctat tgatagaatc atttatttgg agagaaatgt
     5761 atgacttttc cgaggactgc gtattaatgg ttttagccga ctctcattac gatgagaacg
     5821 attacgttag agactacaat gctttcttaa atctggtggc ttaataataa taaggataca
     5881 ataatggtta atttcttaag tttaaaaaat ataaactcac gctataatga ggaattaaaa
     5941 aaagcatgtg caagagttat tgactctggc tggtatgtaa tgggggagga attacaccag
     6001 ttcgaaaacg agttcgctaa atattgtggt gtaaaaggtt ctctgggggt ggcgaatggg
     6061 ctagatgctt tgattctttc attaagagct tggcgtgaaa tggggaaaat taagccgggt
     6121 gatgaggtac ttgttccagc taatacatat atagcttcaa ttattgcaat tacagaaaat
     6181 aatctggtgc ctgtatttgt tgagccggat tataatactt ataatttaga tccagataaa
     6241 attgaatctg cgattactga aaaaacaaaa gtgattttac ctgttcattt gtatggacga
     6301 atttgtccta tggaagaaat attatcattg gcgaaaaaat ataatcttct tgtgttagag
     6361 gattgtgctc aggcgcatgg tgcatctctt aatggcagaa aaacaggggg gtggggagat
     6421 gcgggggctt ttagttttta tccaggtaaa aatttggggg cccttggtga ttctggggct
     6481 attactagca atgatttaga ttttctgaat gtaattcaag ctttaagaaa ttatgggtca
     6541 caccaaaaat atgtaaatca atatattggt gtgaatagtc gattagatga aattcaagca
     6601 gctatgctaa gggttaaatt aaaatacctg gatagtgata acaaaagaag gaaagaaatt
     6661 gctcaatatt acattgattc aataataaat cctttagtcc aattgccgaa atcatgcagt
     6721 aattatgatg aaaatgtttg gcatttattt gttgttaaaa ctcgatatag agagtatctt
     6781 caaaaattcc tgagtaaaca gagtattcaa acattaattc actatccaca ccctccacat
     6841 aaacaaatag cttataagca gtttaattcc atttctttgc cattgacaga aaaaatccat
     6901 aatgaggttt tgtctttgcc aatggatcca actctttctg aagaggagat acaaaaggtt
     6961 gtcaatgcgg taaatggttt tactctatga gttcattttt taagatatca ttctttagtg
     7021 gttgtttgac attattcaag atgttaatgg gctttttaat tgctaaagta atcgctattt
     7081 atacagggcc atctggtatg gccttacttg gtcagcttca aagtttagtc acaggtatta
     7141 atgggatcgt aaatgcacct gtaggaaatg gtatcgttaa atatactgca gagtttaaac
     7201 tagaaggtgc agaatgttgc tctagatggt ggaaaccagc aatcggcttt tcattccttt
     7261 tttgtttggt tttatcgata gtttttatac catttgcaaa taaaatatca tttattttat
     7321 ttggctctta tgaatatgat tatgtggtta ttatagcagt atgtaactta ccttttgcag
     7381 ctttcggtac tatgataatg tctattgtca atggaatggg gaagtataga ctttatatat
     7441 tatatgggtt tatttctaat cttataacgt cgataataat gttacttatg ctaataaaat
     7501 atggtattgt tggtgcatta ttagccactt caatacaata tggtcttatt ggtataattt
     7561 tgatatgttt gaattataat caggaatggt tcaaacataa ttatttttat ccctacgata
     7621 gtatttttaa ttcagaagca aaggatattt tagcttatat attgatggct ataacaagtg
     7681 caattagctt acccttgact ttaatcgtaa ttagaaaaat tctcctaaat atgacggata
     7741 taactaccgt tggacaatgg caggcggtat ggaaaatatc tgaagtatat ataggagtcg
     7801 tgactattgc acttagtact tattttttgc ctaagttgtc tgctctaaat gatacggcta
     7861 aaataaaaaa tgaaattggt attgtcctta aatatactat tccatttgtt tgtgttgccg
     7921 cggttctcat tttcgctttt cgcgatctga taataacgct cttattcacg aatgagtttt
     7981 atcctgcacg tgatcttttt tttattcaac ttgtaggtga tataattaaa ataataagtt
     8041 ggatttatgc attcactatg ttggcttcaa aagcaactag gtggtatgtt ggatctgaat
     8101 tatttttttg tatttcatgg tgtttaatta gttttgttct tgttaatata tataaggcgc
     8161 aaggaattaa tatagcatat tgcattagct atgttatgta ttttttaata atttatctca
     8221 acctaaataa gattataaag aaaaagaatg aacctcattg aaattattaa actccaaatt
     8281 atcaaaactt ctgatattaa taagaaaata agactgctca aggagttggg gggaatgcta
     8341 tgggccaata acgttggcat atacaactta aattcacttg aaagggcagt tatcgaagat
     8401 gtagcaagag attatacgcc acaaattggt agtcaatata aaaagaaaaa aaataatgta
     8461 tttgtgatta ctataggcta catgtctggt gggcatacaa ggttaatgga aaatcttgca
     8521 aatatgctag ataataaatg tgaccttata attactggaa acgcatctgt tacgctaaaa
     8581 aaaaggcttg aaaaatattt caatgaaatt tttgaaattc ctgtttacaa tgacactaac
     8641 attaatgaac tttataagtt aagcgctaat cttgagagat acgaaaatat aatacttaat
     8701 atacaccctg atgatattgc gagcgttatt gcagctggtc ttgcaaaaaa aaatataaac
     8761 acatacgttc actttgttaa tcatgcagat catgttttta gcttcggtcc gaccgtagca
     8821 gatgtctggt atcaaattag ccaattcggg gctaaacttg atcaattaag agagctaagt
     8881 ggtaaaacta cattcttggg gataccgatt gcgataaatg atgatcattt atctaataat
     8941 atgatttcca ctaatgatga agtgaaatgt attatcactg ctggctctgc aaataaatac
     9001 aagccgcata aaggctattc tctcataaaa aaaattcctg aaatattata tcaatttcca
     9061 gaagcatcaa taatagtagt aggtactaac atcctatttt gctattggtg gtggggaact
     9121 tttttaaaat tcagaaagcg tattaaattc tataaagcac ttaatcattc tgaatatctt
     9181 ttacttatgc aaaatgctga tatttatttg gatagttatc ctattcccgg ggggacggca
     9241 tttgttgagc agtcctttct gggtaaaaaa tgtgccgggt atatttcacc gcaacaaggg
     9301 tatacaccac ttgaaaacat aaaatgcgac atcacaagtt ctgagttaaa caaaataaat
     9361 aacgttgata taattgagaa aataaaatat gttcatcctt ttgataatgt aaaaaataga
     9421 cttttaagcg ccatcaacaa acaagtgatg actactataa actgggatta ttattacaaa
     9481 tggactggtg acattaactt ttttaaactc gaaaaaataa gagaaattcc attatattta
     9541 ttgctggaat gtgtgaaaga aaattctagc ttaaaatgta taatatcaca atataaattt
     9601 ataactatta tttctttaat taaaaatttt atcaggctct atgtcagtaa actaaagggg
     9661 aaataatgga atatactatt attattattt tttttgttct gttgtatttt acatctaaag
     9721 ttacgtggcc ctttagtgga aaaaatgcac atccaattaa ctttgtttta ttcacgcaaa
     9781 tatttatatt aacgctgcca ggtgttctta ttttaacatt ttttaaagag tgcgcgggat
     9841 ctataacatg tgagataata gctgaaaaca tcaaaataaa cgtgatgttg gattatttta
     9901 ctgtgctctg ttttatcctt ttgggggtgc tttcttcatt tttcttgtta aaaggtaaaa
     9961 ctgattttcg gccagcatcg aatgcccata gacgatatct aaatacgtgc tttatattaa
    10021 ctctacttat ttttttggta aaaattattt ctgttaatca agttcctcta tttcttacat
    10081 tacttggtca taaagatgct gcagagattc aaaaagctga gatccttaga ggggaagatg
    10141 ggttttcatt tccggtaata aattttctaa taaaatattt tcctttgtat ttttattaca
    10201 gtacgttgat tggtttgttt aaacgcaaag ttactatagt ttactttgta atggcttttc
    10261 ttgtcacgtc tgtgacaatg ctttacgatt tacaaaaagg tccggttgtt attatgatta
    10321 ttggctcatt ttggctttat tgggcttata ccggaaaagc aaaaatgata gtagtaggtg
    10381 ggatattcag tttattttta gtaggtgttc tattttatat ctccttcgat tttggtgatg
    10441 atacccaata ttttattacc gctgtgttaa ataggacctt cattgctcag aatgatggaa
    10501 tgtattgggt atatcaatat tataatatat taccaaataa agaactgtat tctttatggg
    10561 gggtcccact tgcacaacag tttggtatcc cgcagattga cccattaagt gatattattg
    10621 gtgttgtttt tcctcaagct aaggatagtt gggttaatac aacgacattt ttgttaggtg
    10681 aggcaaaggc aattttcggt gactatagtt ccattatctc atctttagta gtatttatta
    10741 atgtgtttgt tgtatgtgtg ctatcaacac ttttagtaag agtatcaaaa aatatttttt
    10801 atcccgcaat attcgttatg attcaaacta taccatttgc aaacaatttg actgacttat
    10861 tatatggtag atttattttt ggttttttag tctttatgtt atttccagtg cttatatgtt
    10921 ttttgtgtta tggaaaatta aaaaatgatg aatagaaaag tgcttattat aatggttacc
    10981 tataatcctg attggagtaa ggtaattaat aaagtgaagt ctttttgtgt tgcaggaaca
    11041 gtttttgttt ctgataatag cgattctgat tcctcatcat tgataaacta tgaaaatgtt
    11101 atatacagat ataatcatgg taatgtaggc atagcaaaag ctcagaattt aggtattgaa
    11161 tatgcgatgc agtataatta tgagtttgta gcttttgttg atgatgatag taatctgagc
    11221 gggggtaaaa ttacacagtt aacaaataat ttatgtaaat taggcagtct tggtgaaaat
    11281 attgcagcat tttgcgctta ccctagtgaa agaggtggtt ttgacaagac aaaaaaaact
    11341 tcgcttggta gacaattatt gttaactaag aatttaatga gttcaggatc tttaacaaag
    11401 gttgatgtat ttaaaaagat aggtttattt gatgaagatt tatttattga ctatgtggat
    11461 tatgaatggg gctggagagc actggaaaaa aattttttaa tcgtaattga tacatcagta
    11521 gaatttgaac attcacttgg tcaggggcgc tcaaaaattg gattagggat cccctctcct
    11581 atccgacatt tttatcaaac tagaaatctt ttatggatat caaggctaaa ttatgtacca
    11641 atatattgga aacttaaaca gtttttactt cttccaattc gctatttcta ttttggtttt
    11701 ttttataatc actctaaatt acggcgaacc cattttctca aagggtttgc cgcagggtta
    11761 aaatttaaat catagtttta tgagataata tcatcaacgg gacgaataat tttagctgga
    11821 ttgccagcaa caattacatt atccggaata tccttcgtaa ctacggagcc tgcgccaata
    11881 attgaatttt caccgatgat aatacccggc aaaattgttg cgttcgcacc aatagaagca
    11941 ttctttttaa tatgtatcgt tggataataa tctgggtatt ttttagatct tggataaata
    12001 tcatttgtaa atgtaacatt tggacctata aaaacattat cttcaattat gactccatcc
    12061 catatataga ctccagattt tatagtgaca ttatcgccaa taataacttt attttcgatt
    12121 aaagtatgtg cgcaaatatt gcagttatta ccaattacag cgccttcaaa aataatgctg
    12181 tattgccaaa tggttgtatt tagaccgatt tttacagttt ttacatcact taatggatga
    12241 attttcataa attctaccct tcatttttaa aacaataata ttgtaaatga tatcatttag
    12301 tattaatgat atcattagac gcctgaatag taaaaagttt tccctttggt tataaagtca
    12361 aaagttaagt agcgtaaatc ggtattctga caggagtaaa acatgtcaaa gcaacagatc
    12421 ggcgtcgtcg gtatggcagt gatggggcgc aaccttgcgc tcaacatcga aagccgtggt
    12481 tataccgtct ctattttcaa ccgttcccgt gaaaagacgg aagaagtgat tgccgaaaat
    12541 ccaggcaaga aactggttcc ttactatacg gtgaaagagt ttgttgaatc tctggaaacg
    12601 cctcgtcgca tcctgttaat ggtgaaagca ggtgcaggca cggatgctgc tattgattcc
    12661 ctcaagccgt acctcgataa aggtgacatc atcattgatg gtggtaacac cttcttccag
    12721 gacaccattc gtcgtaaccg tgagctttct tcagaaggct ttaacttcat cggtaccggt
    12781 gtttccggcg gagaagaggg ggcgctgaaa gggccgtcca tcatgcctgg tggccagaaa
    12841 gaagcctatg aactggttgc gccgatcctg accaaaatcg ccgcagtagc tgaagacggt
    12901 gagccatgcg ttacctatat tggtgccgat ggcgcaggtc actatgtgaa gatggttcac
    12961 aacggtattg aatacggaga tatgcaactg attgctgaag cctactctct gcttaaaggt
    13021 ggcctgaacc tcaccaacga agaactggcg cagacgttta ccgagtggaa taacggtgaa
    13081 ctgagcagct acctgatcga catcaccaaa gacatcttca ctaaaaaaga tgaagacggt
    13141 aactacctgg ttgatgtgat cctggatgaa gcggctaaca aaggtaccgg taaatggacc
    13201 agccagagcg cgcttgatct cggcgaaccg ctgtcgctga ttaccgagtc tgtgtttgca
    13261 cgatacatct cttctctgaa agagcagcgt gttgccgcat ctaaagttct ttctggtccg
    13321 caagcgcagc ctgctggcga caaaggtgaa ttcatcgaaa aagttcgccg tgcgctgtat
    13381 ctgggtaaaa tcgtttctta cgctcagggc ttctctcagc tacgcgctgc gtctgaagag
    13441 tacaactggg atctgaacta cggtgaaatc gcgaagattt tccgtgctgg ctgcatcatc
    13501 cgtgcgcagt tcctgcagaa aatcaccgat gcttatgccg aaaatccgca gatcgctaac
    13561 ctgttgctgg ctccttactt caagcaaatt gccgatgact accagcaggc gctgcgcgat
    13621 gtcgtcgctt acgcagtaca gaacggtatc ccggtgccga ccttcgccgc tgctgttgcc
    13681 tattacgaca gctaccgcgc cgctgttctg cctgcgaacc taattcaggc acagcgcgac
    13741 taa
//
