LOCUS       AB812030               15182 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: E3a.
ACCESSION   AB812030
VERSION     AB812030.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 15182)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..15182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E3a"
                     /db_xref="taxon:562"
                     /note="serogroup:O74"
     gene            <1..207
                     /gene="wcaM"
     CDS             <1..207
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01197.1"
                     /translation="SVGPALSMNFDMRKDVRGVFMAKEETLLSLANVHAVNERGQSSV
                     DIDRINHHIVNVEKINFRLPERRE"
     gene            370..1275
                     /gene="galF"
     CDS             370..1275
                     /gene="galF"
                     /note="O074_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01198.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     gene            1646..2722
                     /gene="rmlB"
     CDS             1646..2722
                     /gene="rmlB"
                     /note="O074_02"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="BAQ01199.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
     gene            2719..3582
                     /gene="rmlA"
     CDS             2719..3582
                     /gene="rmlA"
                     /note="O074_03"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="BAQ01200.1"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIQDILIITTPEDQQGFIRLLGNGSQFGINLNYATQRNPDGLAQAFIIGEQFIGEDSV
                     CLVLGDNIFFGQGFTPKLRCAAEREIGATIFGYQVMDPERFGVVEFDCNYKALSIVEK
                     PNKPKSNWAVTGLYFYDNNVIDIAKKIKPSHRGELEITSVNEVYLKNNELTVELLGRG
                     FAWLDTGTHDSLIEASNFVETVQRRQGMMVACPEEIAWRNGWLSNEDLYKLGTKIKKN
                     LYGKYLIDLVK"
     CDS             3584..3988
                     /note="O074_04"
                     /gene="fdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="putative dTDP-6-deoxy-3,4-keto-hexulose
                     isomerase"
                     /protein_id="BAQ01201.1"
                     /translation="MNVEFIPLQKHGDERGMLVALEQAKNIPFEIKRVYYMFGTQGNV
                     RRGYHAHKKIRQVAIPLNGSCRFHLDNGREKIDVVLDDPALGLLIEPGVWHEMYDYSK
                     NCILLVLASDIYDENDYIRNYEDFMRSVKNDS"
     CDS             3978..4436
                     /note="O074_05"
                     /gene="fdtB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="BAQ01202.1"
                     /translation="MIHKLSDVQTIHIGKKTRVWQYSVILPGAVIGDECNICAHTLIE
                     NDVVIGDRVTIKSGVYIWDGVVIEDDVFIGPCVTFTNDKIPRSKKYPEKFLKTIIKKG
                     ASIGANATILPGITIGENAMVGAGTVVTKDVAPYTVIAGNPGKVIRDIKE"
     CDS             4439..5542
                     /note="O074_06"
                     /gene="fdtC"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminotransferase"
                     /protein_id="BAQ01203.1"
                     /translation="MIDFLNLKRINEKYKAEMQDAFNRVVDSGWYIMGQELKYFESEF
                     AQYCGAKYAIGVANGLDALYLVLKAWKEQGKLSDGDEVLVQANTYIASILAITNNNLV
                     PVFIEPDETTFNLNPAFIRSKITTRTKVILPVHLYGKISPMAEIMDIAEEYKLLVLED
                     CAQAHGAELNKVKAGNWGNAAGFSFYPGKNLGAIGDAGAITTSDDELASILLALRNYG
                     SHVKYENIYQGINSRLDEFQAAVLRVKLPKLDKENERRRQIARKYTAEISNKYMSLPS
                     YTDDLSHVWHLFVVRTQFRKELQLHFSKNEIQTMIHYPIPPHKQLAYTAMSNIHLPVT
                     ERIHNTVMSLPMDPSMTDEHINKVIKVANEFKK"
     gene            5539..6798
                     /gene="wzx"
     CDS             5539..6798
                     /gene="wzx"
                     /note="O074_07"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01204.1"
                     /translation="MRRLLSVTIFTGLLTFMKMCAGFIVSKVVAIYIGPSGLAMLGQI
                     QNIVALFNGLVNSPAGAGVVRFTSQNYDNGDISKCVPWWRASMRWCIIVSSLVIISVI
                     PFNKELSLWLFNDNSYSPVIIITVLILPLTALGTMINSVINGQQKYRVFVSIGMISVI
                     ISTAVMVILIMKFKVFGGLLAVSIQYGVIGFTSLLLCLRFAWVRPNYWFGKTSPDNMK
                     HIFHYMLMAITSAFCLPLSLILIRKILIFYVGWDLTGQWQAVWKISEAYLSVVTLALS
                     TYFLPKLSKLTTYGEIKREITSTVLVIMPLIVIMGIFIYIMRDKIIFLLFTNDFIASI
                     SLFKYQLCGDFIKILAWIFAYPLISQGKVKWYITLEILSAFLFVTLSFIFIQKLGLSG
                     VFFAYIVNYLFYFLFILMNFKRIFKNI"
     CDS             6809..7750
                     /note="O074_08"
                     /gene="wceX"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01205.1"
                     /translation="MHEADIISVIVLAYNSADTITSTLESIAKQEYGAKNIELIISDD
                     ASQDLTISIIENWLVDNYLKFYFVNKIYHHKNLGIVANLDNAYKTASGKWIKVIAGDD
                     ILTSNCLSIYSEFTKKSQGRVFLSYIQSFSQNSIGIVKKDILPPRSQVVLLKHGTLQQ
                     QKKHLMNFSFSATPSLFIEKKLLDDIGYLDKKYRLMEDYPLWHKITDYGERIYFVPEI
                     TVLYRVQESVSRSKEKIVNLDFLTDILRFEKILIKRLSPVSITYHRRKLWCYLYPLLI
                     RLVNNKKNIFSRGCIFLLNVILKPLYLSTIVRRYFEK"
     CDS             7763..8587
                     /note="O074_09"
                     /gene="wdbM"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01206.1"
                     /translation="MMDNNKHTVAVIMSVYDGDEPYYFEKAIQSIQKQTYQSIDIYLY
                     IDAVQRKDLLNRIRHYKKCSRFHVVTGDKKRGLAYGLNRLLDKIRGKGYGYIARMDSD
                     DISTTDRIESQVRFLEKNKNIDVIGTNCIEIDGDDNEIFYKKMPETHHELIKSIVKRS
                     PFIHPSVMFRGSVVEHIRYNDSLMNTQDYYLWIDLLSKKYKFHNIQQPLLFFRVNDSF
                     YARRGFGKLINEVKSRLYAMKKLNKKNVKNYLYILALICLRLAPGNIKKYCYMRFR"
     gene            8587..9762
                     /gene="wzy"
     CDS             8587..9762
                     /gene="wzy"
                     /note="O074_10"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01207.1"
                     /translation="MNCIMFFYGVFFALPLSILNVLPYGMKIDDVILFIALIVFPILS
                     FKKGYHVTIPLSCLLFIIITVLFASLSFLKGINSTNVFLGDSAGTVIGRIIQSLLILI
                     LITFLKNTPHYLLSFFKGYVIAATLSLIIFFGYYVTHINISSFTMRGVYFAKDIFQYN
                     DDLPFTVHVNTLGSFFLIAFFLLKYNFPKYQLLSYLFLLPSLLLISKGDMLAILTYFA
                     YVFYKRNNLNAFLSLIGLLILVLLMPYLYNVYLSLSDYRVYASGRDELYGASLSSIID
                     NPFGYGLGMQNNILYSITGIDFPAHNILLSIGIELGLLYLFLLVAYIIGWLLLGKVDY
                     KIIFISFLVIGMFGNAMYFYKFHSLAIALCFLSVWHINFNHKTKQVDLINNENTLHS"
     CDS             9770..10822
                     /note="O074_11"
                     /gene="wdaB"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01208.1"
                     /translation="MLTGPNIVALTNIEHIHDQGYDVSICFLNNGDDLNNIFPFLKEI
                     EVFYLNLKSLGFISGLKNLNYYINSIKPTIIHSHCFLPDIYNIFNKFFCGPIKNKRVT
                     TIHNIPKEDYRLRYGRIKGSLLLYFHKALFPFFDNCICISKTVQKSIGIKKTSVIYNP
                     VRDVFFNSTKDDVECLTIVYCGHFSQLKNPITLIQLLADSKVNFQFLGIGDGPLLYQC
                     RQLVKKDPRFTFVGRVNHVHKFYEKANCLIHISQTEGFCLSVAEALASNMYVITNELP
                     VFLELKQALSSEGFYMLNDINNYNLEMVLNSINRKIEAGERCNSGASNRVKALLSPTF
                     IAQQHIVLYQKLTVEK"
     gene            10915..12321
                     /gene="gnd"
     CDS             10915..12321
                     /gene="gnd"
                     /note="O074_12"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01209.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFIESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAILPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            12570..13736
                     /gene="ugd"
     CDS             12570..13736
                     /gene="ugd"
                     /note="O074_13"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01210.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSDRAERFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     CDS             complement(13802..14806)
                     /note="O074_14"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide sugar epimerase"
                     /protein_id="BAQ01211.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPQWTVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
BASE COUNT     4659 a   2633 c   3124 g   4766 t
ORIGIN
        1 tctgttggac ccgcattgag catgaacttc gacatgcgca aagacgttcg cggcgtcttt
       61 atggcgaaag aagaaacact gctgtctctt gcaaatgttc atgcggtgaa tgaaagaggg
      121 caaagctccg tcgatatcga caggattaat caccatattg ttaatgtgga aaagattaac
      181 tttagattgc cggaacggag agagtagatt tgcgaccatt cctggaaaaa tggagccata
      241 cttaggaaca atgctactgc aatccacaac gaagcggcgt aacatcacaa gtaattcagt
      301 aatcaattca aggtaattga tgctggcgaa aaaaatcgaa caagctataa ttcagcaacc
      361 attttacagg tggatgaaat aatgacgaat ttaaaagcag ttattcctgt agcgggtctt
      421 gggatgcata tgttgcctgc cactaaggcg attcccaaag agatgctacc gatcgtcgac
      481 aagccaatga ttcagtacat tgttgacgag attgtggctg cagggatcaa agaaatcctc
      541 ctggtaactc acgcgtccaa gaacgcggtc gaaaaccact tcgacacctc ttatgaatta
      601 gaatctctcc ttgaacagcg cgtgaagcgt cagctgctgg cggaagtgca gtccatctgt
      661 ccgccgggtg tgaccattat gaacgtgcgt cagggcgaac ctttaggttt gggccactcc
      721 attttatgtg cacgacccgc cattggtgac aacccatttg tcgtggtgct gccagacgtt
      781 gtgatcgacg acgccagcgc cgacccgctg cgctacaacc ttgctgccat gattgcgcgc
      841 ttcaacgaaa caggacgcag ccaggtgctg gcaaaacgta tgccgggtga cctctctgaa
      901 tactccgtca ttcagaccaa agaaccgctg gatcgcgaag gcaaagtcag ccgcatcgtg
      961 gaatttatcg aaaaaccgga tcagccgcag acgctggact cagacatcat ggccgtgggt
     1021 cgctatgtgc tttctgccga tatttggccg gaacttgaac gcactcagcc tggtgcatgg
     1081 gggcgtattc agctgactga tgccatcgct gaactggcga aaaaacagtc cgttgatgcc
     1141 atgctgatga caggtgacag ctacgactgc ggtaaaaaaa tgggttatat gcaggcattt
     1201 gtgaagtatg gactacgcaa cctcaaagaa ggggcgaagt tccggaaagg gattgagaag
     1261 ctgttaagcg aataatgaaa atctgaccga atgtaacggt tgataagaaa attataacgg
     1321 cagtgaagat tcgtggcgaa agtaatttgt tgcgaatatt cctgccgttg ttttatataa
     1381 acaatcagaa taacaacgag ttagcaatag gattttagtc aaagttttcc aggattttcc
     1441 ttgtttccag ggcggattgg taagacaatt agcgtttgaa tttttcgggt taagcgcgag
     1501 tgggtaacgc tcgtcacatc gtagacatgc atgcagtgct ctggtagctg taaagccaag
     1561 ggcggtagcg tgcattaata cctctattaa tcaaactgag agccgcttat ttcacagcat
     1621 gctctgaaga atatggaata ataaagtgaa gatacttgtt actggtggcg caggatttat
     1681 tggttctgct gtagttcgtc acattataaa taatacgcag gatagtgttg ttaatgtcga
     1741 taaattaacg tacgccggaa acctggaatc gcttgctgat gtttctgatt ctgaacgcta
     1801 tgtttttgaa catgcggata tttgcgatgc agctgcaatg gcacggattt ttgctcagca
     1861 tcagccggat gcagtgatgc acttggctgc tgaaagccat gttgaccgtt caattacagg
     1921 tcctgcggca tttattgaaa ccaatattgt tggtacttat gtccttttgg aagccgctcg
     1981 caattactgg tctgctcttg atagcgacaa gaaaaatagc ttccgttttc atcatatttc
     2041 tactgacgaa gtctatggtg atttgcctca tccagatgaa gtaaataata acgaagaatt
     2101 acccttattt actgagacga cagcttacgc accaagcagc ccttattccg catcaaaagc
     2161 atccagcgat catttagtcc gggcgtggaa acgcacctat ggtttaccga ccattgtgac
     2221 taactgttcg aataactacg gtccttatca ctttccggaa aaattgattc cactagtaat
     2281 tcttaatgct ctggaaggta aggcattacc tatttatggc aaaggggatc aaattcgtga
     2341 ctggctgtat gttgaagatc atgcgcgtgc gttatatacc gtcgtaaccg aaggtaaagc
     2401 gggtgaaact tataacattg gtggacacaa cgaaaagaaa aacatcgatg tagtgctcac
     2461 tatttgtgat ttgttggatg agattgtacc gaaagagaaa tcttaccgcg agcaaattac
     2521 ttatgttgcc gatcgcccgg gacacgatcg ccgttatgcg attgatgcag agaagattag
     2581 ccgcgaattg ggctggaaac cgcaggaaac gtttgagagc gggattcgta aaacaattaa
     2641 ctggtatttg aataataatg attggtgccg gcgagttcaa gatggctctt atcaacgtga
     2701 gcgtttgggg gtaattagat gaaaggaatt attttagctg gtggttctgg aacaaggctt
     2761 tatccaatta caatgggagt ttcaaagcag ctattaccca tttatgataa accgatgatt
     2821 tattatcctt tgtcagtgct gatgttggca ggtatacaag atattcttat tattacaacg
     2881 ccagaagatc agcaggggtt cataagatta cttgggaacg gaagccaatt tggtattaat
     2941 cttaattacg ccactcaacg gaatcctgat ggtttagctc aggccttcat catcggggaa
     3001 caatttattg gtgaggattc tgtctgctta gttttgggag ataatatttt ttttggtcaa
     3061 ggatttactc cgaaacttag atgtgccgct gagagggaaa taggagccac gatatttggt
     3121 tatcaggtaa tggatcctga acgattcggt gtggtcgaat ttgattgcaa ttataaagca
     3181 ttaagtattg ttgaaaaacc aaataaacca aaatcaaact gggctgtaac tggattgtat
     3241 ttttacgata acaatgttat tgatattgca aaaaaaataa aaccgtcgca tcgtggtgaa
     3301 ttagaaatca catctgttaa tgaggtttac ttaaaaaata atgagttaac tgttgaatta
     3361 ttaggtcgag ggtttgcgtg gctggatacc ggcacacatg atagtctcat tgaagcatct
     3421 aattttgtcg aaaccgttca acgtagacaa ggaatgatgg ttgcatgccc cgaagaaata
     3481 gcttggcgga atggttggtt atctaatgaa gatttatata aattaggcac gaaaattaaa
     3541 aagaatctat acggaaaata tcttatcgat ttggtgaaat aatatgaatg tcgagtttat
     3601 tccattacaa aaacatggtg atgaaagagg gatgttagta gccctagagc aagccaaaaa
     3661 cattccattt gaaattaaac gtgtttatta tatgtttgga actcaaggaa atgttcggcg
     3721 cgggtatcat gcccataaaa aaattaggca agtagcgata ccgctaaatg gttcatgccg
     3781 ttttcattta gataatggtc gtgagaaaat agatgttgtt ttagatgatc ctgcattagg
     3841 gttgcttata gagcctggag tctggcatga aatgtacgat tactcaaaga actgtattct
     3901 tctagtattg gcgagcgata tttatgatga aaatgactat attagaaatt atgaggattt
     3961 tatgaggtct gttaaaaatg attcataaat taagcgatgt tcaaactatc catataggta
     4021 aaaaaacaag ggtctggcaa tattcagtta tattaccggg tgctgtaatt ggtgatgagt
     4081 gtaacatatg tgcacataca cttattgaaa atgatgttgt aattggggat agagttacta
     4141 ttaaatcggg cgtatatatc tgggatgggg ttgttattga agatgatgta tttatagggc
     4201 cctgtgtaac atttaccaat gataaaatcc ccaggtcaaa gaaataccca gaaaaatttc
     4261 ttaagactat tattaaaaaa ggtgcttcca ttggagccaa tgcaactata ttacctggaa
     4321 taacaattgg tgaaaatgct atggttgggg ctggtaccgt agtcactaaa gatgttgcac
     4381 cttatacagt cattgcaggt aatccaggta aagttattag ggatattaag gaataaaaat
     4441 gatcgacttt ctcaacttga aaagaattaa tgaaaaatat aaagcggaaa tgcaagatgc
     4501 ctttaatcga gttgtcgatt ctggctggta tattatgggg caagaattaa aatattttga
     4561 aagtgaattt gcacagtatt gcggggcaaa atatgctata ggtgtagcta atggtttaga
     4621 tgccctttat ctcgttttaa aagcgtggaa agaacaaggt aaattgagtg atggcgatga
     4681 agtgctggtg caggcaaaca catatattgc ctcaattctt gctataacaa ataataatct
     4741 cgttccggtg ttcattgaac cagatgaaac gacttttaat ttaaatccgg catttattcg
     4801 ttcaaaaata acaactcgga caaaagtgat attgcccgtg catctttatg gaaaaatatc
     4861 accaatggct gaaataatgg acatcgctga agagtacaag ctattggtcc tagaagattg
     4921 tgctcaagct catggtgctg agttgaataa agtcaaagca ggcaattggg gaaatgctgc
     4981 cggatttagt ttttatccgg gtaaaaatct tggggccata ggtgatgcgg gggctattac
     5041 aacatcagat gatgagcttg ccagtatttt gttagcatta agaaattatg gttcccatgt
     5101 taaatatgaa aatatttacc aaggtataaa cagtcgcttg gatgaatttc aggctgcagt
     5161 acttcgagtt aaattaccta aattagataa agaaaatgag agaaggcgtc aaatagctag
     5221 aaagtatact gcagaaatta gcaataaata catgtcatta ccttcataca ctgacgattt
     5281 atcacatgta tggcatttgt ttgttgttag aactcaattt agaaaagaat tgcagcttca
     5341 tttttcaaaa aatgaaatac aaactatgat acattatccg atacctccgc ataaacaatt
     5401 agcatatact gctatgagta atatacattt acccgtaacc gaaagaatac ataacactgt
     5461 tatgtcatta cctatggatc catcaatgac tgatgagcac ataaataaag ttattaaagt
     5521 tgctaatgaa tttaaaaaat gaggagacta ttatcagtaa caatatttac aggcctgctg
     5581 acttttatga aaatgtgtgc aggctttatc gttagcaagg ttgttgcaat atatataggc
     5641 cctagtgggc tggcgatgct tggacagata cagaatattg ttgctctttt caatgggctg
     5701 gttaactccc cggcaggagc tggggttgta cgtttcactt ctcaaaatta tgacaatggt
     5761 gatatttcta aatgtgtgcc atggtggcgc gcaagcatgc gctggtgcat aatagtttct
     5821 tctttagtaa taatttcagt tatcccattt aataaagaat tatcattatg gttatttaat
     5881 gataattctt attcaccagt tatcattata actgttttaa tactcccttt aactgcgtta
     5941 ggaacgatga taaattccgt tattaacggc caacaaaaat atagagtttt tgtttctatt
     6001 ggtatgatat ccgttatcat atcaactgca gtaatggtca tacttataat gaagttcaag
     6061 gtatttggag ggttattagc agtttcaatt caatatggag taattggttt tacttcttta
     6121 ctattatgct taaggtttgc atgggttaga cctaattatt ggtttggtaa aacgtcgcca
     6181 gacaatatga agcatatttt tcattatatg ttgatggcaa ttacaagcgc attttgcctt
     6241 ccactatctc ttattctgat aaggaagata cttatatttt atgttggttg ggatttgact
     6301 ggtcaatggc aagctgtatg gaaaatatct gaggcttacc taagcgtggt aactcttgca
     6361 ttatcaacat attttttacc aaaattatct aaacttacaa catatggtga gattaaaaga
     6421 gaaataacat caacggtttt agtaataatg ccattgatag taattatggg aatatttata
     6481 tatatcatgc gtgataaaat tatctttctt ttattcacga atgattttat agcatcaata
     6541 agcttattca agtatcaatt atgtggtgat tttattaaaa tattggcttg gatttttgca
     6601 tatcctttga tttcacaggg aaaagtaaag tggtatatta cattagagat cttatcagcc
     6661 ttcctttttg ttaccttgag ctttatattt atccaaaagc ttggattgtc tggagtattt
     6721 tttgcataca tagttaatta tttattttat tttctattta tactgatgaa ctttaaaaga
     6781 atatttaaga atatatgagg atattatttt gcatgaggct gatataattt ctgttattgt
     6841 cttggcatac aattctgctg atactataac atctacatta gaaagtatag ctaaacaaga
     6901 gtatggtgca aaaaatattg agttaataat aagtgatgat gcttctcagg atttaacaat
     6961 atctatcata gaaaactggt tagttgataa ctatcttaag ttttattttg ttaataagat
     7021 atatcatcat aaaaatctag ggatagtagc taatttagat aatgcatata aaaccgcaag
     7081 cggtaaatgg ataaaggtaa ttgcaggtga tgacatacta acctcgaact gtttatcaat
     7141 atattctgaa tttacaaaaa aatctcaagg acgagttttt ttatcgtata tacagtcatt
     7201 ttctcaaaac agcattggaa tagttaagaa ggatattctc ccaccgagat ctcaggtggt
     7261 tctgcttaaa catggtactc tgcagcaaca aaaaaaacat ttgatgaatt ttagtttttc
     7321 tgccactccg tcattgttta tcgagaaaaa attattggat gatatagggt atctcgataa
     7381 aaaatatcgg ctaatggaag attatcctct ctggcataaa ataactgatt atggtgaaag
     7441 aatttatttt gttccagaga tcactgtgtt gtatagagtg caggaatcag ttagtaggtc
     7501 aaaggagaag attgtaaatc tagatttttt aacagatatt ctaagatttg aaaagatatt
     7561 aatcaaaagg ttatctccag ttagtattac ttatcataga agaaaactat ggtgctattt
     7621 gtacccatta ttaattagac ttgttaataa taaaaaaaat atattttcta gaggctgtat
     7681 ttttctacta aatgtaattc ttaaaccatt atatttgtca acaatagtta gacgatattt
     7741 tgaaaaataa aaaggtttca tagtgatgga taataataaa catacggttg ctgtcattat
     7801 gtcagtttat gatggagatg agccgtacta ttttgaaaaa gctattcaga gtatccaaaa
     7861 acaaacctac caaagtatcg atatatattt atatatcgat gcagtgcaaa ggaaagacct
     7921 tctgaacagg attcgacact ataaaaaatg ctctaggttt catgttgtaa caggtgataa
     7981 aaaaagaggt ttagcatatg ggttaaatcg tcttttagac aaaatccgag gaaaaggata
     8041 tggatatatt gccagaatgg attctgatga tatttctacg actgatcgca ttgaatctca
     8101 ggtgagattt ctagaaaaaa ataaaaacat agatgttata ggtacaaatt gtatagaaat
     8161 agatggggat gataatgaga ttttttataa aaaaatgcca gaaactcatc atgaactaat
     8221 aaaatccata gttaagcgct ctccatttat tcatccttca gttatgttta gaggaagtgt
     8281 tgttgagcac attagatata atgatagtct tatgaatacg caagattatt atttgtggat
     8341 tgatttatta tctaaaaaat ataaatttca taatatccaa caaccattat tattttttcg
     8401 tgttaatgat tcattttacg ctagacgtgg gtttggaaaa ttaatcaatg aagtgaaaag
     8461 tagattgtac gcgatgaaga agttaaataa aaaaaatgtt aaaaattatt tatacattct
     8521 agcgttaata tgtttacgcc tcgcaccggg taatataaag aaatattgtt acatgaggtt
     8581 ccgataatga attgtatcat gtttttttat ggtgtttttt tcgcattgcc gttatctatt
     8641 ctcaacgtgt taccttatgg tatgaaaata gatgatgtaa ttctttttat tgcattaatt
     8701 gtatttccaa ttttaagttt taaaaaaggt tatcatgtga caattccatt gtcatgttta
     8761 ctatttatta tcataactgt gctgtttgcc tcactgtctt ttttgaaggg aataaatagc
     8821 acgaatgtat ttttagggga ttctgcggga actgttattg gtcgtataat tcaatctcta
     8881 cttattctca tattgattac ttttttaaaa aacaccccgc attatttatt aagttttttt
     8941 aaaggttatg ttatagctgc aaccttatcc ctcattattt tttttggtta ttatgttacg
     9001 catataaata tatcttcatt tactatgaga ggggtatatt tcgctaagga tatttttcaa
     9061 tacaatgatg acttaccctt tactgttcat gtgaacacat taggttcttt ttttctaata
     9121 gcgttttttt tattaaagta taacttccct aaataccaat tattatctta tttgttttta
     9181 ttgccttcac tactattaat ctccaaaggt gatatgttgg caatattaac ttattttgct
     9241 tatgtgtttt ataagagaaa taatttaaat gctttcttat ctctcattgg tctattgatc
     9301 cttgtattat tgatgccata tttatacaat gtctacttaa gcttatctga ttatagagta
     9361 tatgcatcag gtagagatga gctatatggt gcgtcactat ctagtataat agataatcca
     9421 tttgggtatg gtctgggaat gcaaaataat atactatatt cgataacagg cattgacttt
     9481 cctgcgcata atattctttt aagtataggg attgaacttg gccttctata tttatttttg
     9541 ttggttgcat atataatcgg ctggttatta ttaggcaaag tagattataa aatcatattt
     9601 atttcttttt tagtaatcgg aatgtttggt aatgcaatgt atttttataa gtttcattca
     9661 cttgctatag cgctttgttt tctgagtgta tggcatatca attttaatca caaaactaaa
     9721 caagttgatt taattaataa tgaaaatact ttacattctt aataaatctg tgctgacagg
     9781 accaaatatc gttgcattaa caaacattga acatattcat gatcagggat atgatgtttc
     9841 tatttgtttt ttaaataacg gtgacgatct aaataatata tttccttttt taaaagaaat
     9901 tgaagttttc tatcttaacc taaaatccct tggttttatt tctgggctaa aaaatttaaa
     9961 ttattatatt aattcaatta aaccaacaat aattcacagc cattgctttc tacctgacat
    10021 ttataacata tttaataagt ttttttgcgg gccaataaaa aataagcgtg ttactacaat
    10081 tcacaacata cctaaagaag attacagatt acgatatggt cgtattaaag gaagtctttt
    10141 gctttatttt cataaggcac ttttcccttt ttttgataat tgcatatgta tctcaaaaac
    10201 tgtacaaaag agtattggga ttaaaaaaac aagtgtgatt tataatcctg ttcgtgatgt
    10261 tttttttaac tcaacgaaag atgatgtaga atgtttaaca attgtatact gtggtcattt
    10321 ctctcaatta aaaaacccta ttacattaat tcagttgtta gccgattcaa aagtgaattt
    10381 tcagttttta ggaatcggtg atggtcctct tttatatcaa tgtagacaac tggttaaaaa
    10441 agatccgcgt tttacttttg tcggtagagt aaatcatgtc cataaatttt atgagaaagc
    10501 aaattgttta atacacattt cacaaactga aggtttttgt ctttcagttg ctgaggctct
    10561 ggcttctaat atgtatgtca ttacaaatga attacctgtt tttttagaac taaagcaagc
    10621 tttgagttca gagggctttt acatgcttaa tgatataaat aattacaatt tagaaatggt
    10681 attaaattct attaatagaa aaattgaagc aggtgaaaga tgtaattctg gtgcgtcgaa
    10741 tagagttaaa gctttacttt ctcctacttt tatagcacag caacatattg tgctttatca
    10801 aaaattgaca gtggaaaagt gagatttcat aatttttgta ctcgtatcta tattattgga
    10861 tgttgtcaac ttgtagcgta gtttagattc atgcccccct gacaggagta aacaatgtca
    10921 aagcaacaaa tcggcgtcgt cggtatggca gtgatggggc gcaaccttgc gctcaacatc
    10981 gaaagccgtg gttataccgt ctctattttc aaccgttccc gtgagaaaac ggaagaagta
    11041 attgccgaaa atccaggcaa gaaactggtt ccttactata cggtgaaaga gtttattgaa
    11101 tctctggaaa cgcctcgtcg cattctgtta atggtgaaag caggtgcagg cacggatgct
    11161 gctattgatt ccctcaagcc atacctcgat aaaggtgaca tcatcattga tggtggtaac
    11221 accttcttcc aggacaccat tcgtcgtaac cgtgagcttt ctgccgaagg ctttaacttc
    11281 attggtaccg gtgtctccgg tggtgaagaa ggcgcgctga aaggtccttc cattatgccc
    11341 ggtgggcaga aagaagccta tgaactggtt gcgccgatcc tgaccaagat cgccgcagtg
    11401 gctgaagacg gtgagccatg cgttacctat attggtgccg atggcgcagg tcactatgtg
    11461 aagatggtcc acaacggtat tgaatatggc gatatgcagc tgattgctga agcctattct
    11521 ctgcttaaag gtggcctgaa cctcaccaac gaagaactgg cgcagacgtt taccgagtgg
    11581 aataacggtg aactgagcag ctacctgatc gacatcacca aagatatctt caccaaaaaa
    11641 gatgaagacg gtaactacct ggttgatgtg attctggatg aagcagcaaa caaaggtaca
    11701 ggcaaatgga ccagccagag cgcgctggat ctcggtgaac cactgtcgct tattaccgag
    11761 tctgtgtttg cacgttatat ctcgtctctg aaagatcagc gcgttgccgc gtctaaagtt
    11821 ctctctggcc cgcaagcgca gccagctggc gacaaggctg agttcatcga aaaagttcgc
    11881 cgtgctctgt atctgggcaa aatcgtttct tacgcccagg gcttctctca gctacgcgcc
    11941 gcgtctgaag agtacaactg ggatctgaac tacggcgaaa tcgcgaagat tttccgtgct
    12001 ggctgcatca tccgtgcgca gttcctgcag aaaatcaccg atgcatatgc cgaaaatccg
    12061 cagatcgcta acctgctgct ggccccgtac ttcaagcaaa ttgccgatga ctatcagcag
    12121 gcgctgcgcg atgttgtcgc ttacgcagta cagaacggta tcccggttcc gaccttcgcc
    12181 gctgcggttg cctattatga cagctaccgc gccgctattc ttcctgcgaa cctgatccag
    12241 gcacagcgtg actatttcgg tgcacatact tataagcgca ttgataaaga aggtgtgttc
    12301 cataccgaat ggctggatta atctgattta aatcaattaa tcaatgcaag gcccggagaa
    12361 gccctccggg cttttttatt atacaaagcg gcaggttatg gccttttttt ataatttata
    12421 gttaaaaacg cgatataata cagcgccaca cagcaggatc gctgccttga cagttcatct
    12481 acttcagcgt taaaaatccc gcagtagatg aagttgtggt ggtggattaa tgaccactct
    12541 aaatgtttaa ccggaagaag tcagagctaa tgaaaataac aatttcagga acaggttatg
    12601 ttggtctttc aaatggtatt ctgattgcgc aaaatcacga agtggttgca ctggatatcg
    12661 ttcaggccaa agtggacatg cttaacaaga agcagtcacc gattgttgat aaggagattg
    12721 aagagtatct ggcgactaaa gatctcaatt tccgtgctac gacagataag tatgacgcgt
    12781 ataaaaatgc cgattacgtt attattgcca cgcctaccga ttatgacccg aaaacaaatt
    12841 attttaatac ctcaagcgtg gaagcggtca ttcgcgatgt gacagaaatt aatcccaacg
    12901 cggtaatgat tataaaatca actatccctg ttggttttac agagtccatt aaagaacggt
    12961 ttggtattga aaatgtgatc ttttcgccgg agtttttacg cgaagggaaa gcactttatg
    13021 ataacttaca cccatcacgc attgtgattg gcgagcaatc cgaccgcgca gaacgttttg
    13081 cagcgttatt acaggaaggc gctattaagc aagacatacc aacattgttt actgactcaa
    13141 ccgaggctga ggcgattaaa ctttttgcca acacctatct ggcgatgcgt gtagcgtatt
    13201 tcaatgaact tgatagttat gctgaaagcc tggggcttaa ttcacgccag attattgagg
    13261 gcgtatgcct tgacccgcgt atcggtaatc actacaacaa cccgtcattc ggttatggtg
    13321 gttattgtct gccgaaagat accaagcagt tactggcaaa ctatcaatct gtaccgaata
    13381 atctgatttc ggcaattgtt gatgctaacc gcacgcgaaa agactttatt gccgattcta
    13441 tccttgcacg taaaccgaaa gttgttggcg tttatcgttt gattatgaag agtggttcag
    13501 acaatttccg tgcttcctcg attcagggta ttatgaagcg aatcaaggcg aaaggtgtgc
    13561 cagtaatcgt ttatgagcca gcgatgaaag aggacgattt tttccgttcg cgcgtggtac
    13621 gtgatctgga tgcgttcaaa caagaagctg atgttattat ttctaaccgt atgtctgccg
    13681 atctggctga tgtagcagat aaagtttata cgcgcgactt gtttggcaat gattaattat
    13741 tttgttttat tctaagaaaa ggccctgata tattagggcc ttttcttatg gttttgtaaa
    13801 atcaaacttt atagaagtta cgataccatt ctacaaagtt ctttaccccc tctttaactg
    13861 acgtttcagg tttgaatcct attacgtcat acagtgcttt tgtatcagca ctggtttcca
    13921 gtacatcacc gggttggaga ggcatcatat tcttgttggc ttcaataccc agagcttctt
    13981 ctaaagcatt gatatagtcc atcaactcca caggcgaact attaccaatg ttatagacac
    14041 gatatggtgc tgaacttgtt gcaggcgatc cagtttctac agtccactgt ggatcttttt
    14101 ctggaataac atcctgtaag cgaataatag cttcggcaat atcatcaata taagtaaagt
    14161 cacgcttcat tttgccgaag ttgtaaacat caatgctttt accttccagc atggctttag
    14221 taaatttaaa taatgccata tccggacgtc cccatggacc ataaaccgta aagaaacgca
    14281 gccctgtggt cggtaagcca tacaaatgag aatacgtatg ggccatgagt tcattcgctt
    14341 ttttagttgc tgcataaagc gaaacaggat gatctacaga gtcatctgta gagaaaggca
    14401 tcttgcggtt catgccataa acagaactgg aggaagcgta aagtagatgc tgaacattat
    14461 tatggcggca tccttccagt atgttcagga atccaatcag gtttgcatct gcatatgcat
    14521 tgggattttc aagggagtaa cgtacaccgg cttgcgcagc gaggtttatt acgcgatcga
    14581 accgctcgtc tgcaaacagt gccgccattt tctcgcgatc ggccaggtca attttataaa
    14641 aactgaaatt gtcgtgcttg agtaaatcaa gtcgtgcttg tttgaggttg acatcgtaat
    14701 aatcatttaa gttgtcaatc cctacaacct gatgaccagc tgcaagaagc cgtttactta
    14761 catagaaacc gataaagcca gcagctcccg taaccagaaa tttcatttat aatcctcgct
    14821 caggctagaa tatagccaat tttcatctgg cataactgaa agttaaatca taccgttaga
    14881 caagaaaaaa agataatcgg tatcagttct aaacctggct gttttttctg gtaacgtgct
    14941 cattttacaa tcaaagctgt tctaagctga ctatacaagc cgacgtcatt atctccaacc
    15001 gtatggcaga agagcttaag gatgtggcag acaaagtcta cacccgcgat ctttttggca
    15061 gcgactaaca tcttgttatc agggcgattt tcgccctgat tctcttatgt tccctttgta
    15121 ataattcatt atttttatca tttatcctat agcattcatg gcgattatcg ctaaactatg
    15181 gc
//
