LOCUS       GU299795               12945 bp    DNA     linear   BCT 08-JUN-2010
DEFINITION  Escherichia coli strain G1720 O antigen gene cluster, complete
            sequence.
ACCESSION   GU299795
VERSION     GU299795.1  GI:288816241
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12945)
  AUTHORS   Li,D., Liu,B., Chen,M., Guo,D., Guo,X., Liu,F., Feng,L. and Wang,L.
  TITLE     A multiplex PCR method to detect 14 Escherichia coli serogroups
            associated with urinary tract infections
  JOURNAL   J. Microbiol. Methods 82 (1), 71-77 (2010)
   PUBMED   20434495
REFERENCE   2  (bases 1 to 12945)
  AUTHORS   Li,D., Liu,B., Chen,M., Guo,D., Guo,X., Liu,F., Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..12945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G1720"
                     /db_xref="taxon:562"
                     /note="serogroup: O75"
     misc_feature    1..12945
                     /note="O antigen gene cluster"
     gene            591..1676
                     /gene="rmlB"
     CDS             591..1676
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ADC54962.1"
                     /db_xref="GI:288816242"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIEVVLTICDL
                     LDEIVPKEKFYCEQINYVADRPGHYCRFAIDAEKVSRELGWKPPETFESGICKTVEWY
                     LSNTKWVDNVKSGAYPSWIEQKYEGRP"
     gene            1676..2575
                     /gene="rmlD"
     CDS             1676..2575
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ADC54963.1"
                     /db_xref="GI:288816243"
                     /translation="MNILLFGKTGQVGWEIKRALAPLGNLIALDVHSPDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRMALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2633..3511
                     /gene="rmlA"
     CDS             2633..3511
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ADC54964.1"
                     /db_xref="GI:288816244"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLSDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDSGQVRVLAEPL
                     KKNAYGQYLLKMVKGY"
     gene            3516..4067
                     /gene="rmlC"
     CDS             3516..4067
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ADC54965.1"
                     /db_xref="GI:288816245"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHFQLPPFEQAKLVRCIVGEVFDVAVDIRPNSETFGSWVGVT
                     LSSENKRQLWIPEGFAHGFLTLSEIAEFVYKTNNYYSSNHERGVIWNDEEINIAWPSQ
                     SEKILSQKDINLPSFRFVQMFSK"
     gene            4201..5304
                     /gene="wekX"
     CDS             4201..5304
                     /gene="wekX"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WekX"
                     /protein_id="ADC54966.1"
                     /db_xref="GI:288816246"
                     /translation="MTKIVVVSTAPIFPTNNGYKSSVLGRIDELLNEGNEVVLIEINL
                     EKVTEKKDELIPTRFNNVQRYEVKKISRSFIAELQILFDIRTRYEQLFSSADIRDNIK
                     KIIDLEKPSIIIAESIWALQALPIEISARIHCVIHDVAIDFFKEMFVSHNEVVRKILF
                     FNDYLKLKITEENIIKRLRVEQFIFLTEEDKCWYKTRYNIDECCCSLASNHLYVEKIK
                     RTINFQTPFLLIPGSIEFSQNFYGLNWFIKNIYPRLNREIRIVVTGKASDKKIKMLNC
                     GEEITFTGELDFYTYNQLSSTCLGVIAPITTGTGIKIKILEAVQKGIPVLTTKFASKG
                     ICSDLCFYCEEDTDTNFVNLINSFLETTLRVQE"
     gene            5301..6374
                     /gene="wzy"
     CDS             5301..6374
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADC54967.1"
                     /db_xref="GI:288816247"
                     /translation="MNLLLFSVLAFGLILALAHNNKSGDINAYLMFFLVVLMVLISGL
                     RMNDSDYIEYKKMYNEVPILCDFSLASIRDIHGEVGYLFLSSIFKTLCLPFQVFLFFI
                     AFLSLLLTYFSFRKISLIPILSLVFYLSHAFIVRDLIQIRAGLAVSISLYSIIKFKGN
                     KSIITGVLFASLIHSGALIIALCYPFFKKKYITLKMMLFLFLVSVIFSYLNGLNLSIQ
                     LLSQYSLLPTAISNYVGWEEYDYRVSIFTNPVFIKGVFLIVLMHKYVLSDIKNEKIIV
                     LYNLYVLGVLAMVALSGMAILSGRLSSFLTLGESILIVYALFYKRNTSLAFLIFSFLT
                     IVQLGYDLFISNVHPELTLIIFG"
     gene            6381..7469
                     /gene="wekY"
     CDS             6381..7469
                     /gene="wekY"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WekY"
                     /protein_id="ADC54968.1"
                     /db_xref="GI:288816248"
                     /translation="MKNNKIGILISKIQNLGPVNVVRGLIKENKKYAFTVFCLTNSVD
                     KNIYDELCCLGAKIVLIPDGTWFSKILFVRNFLKEHPHNILHSHGITADMFSYFLNGV
                     KISTIHNRLDEDYIPLFGAVKGNAIYYLHRFILRRFNHIVACSAAVQSKLKQSKVKTK
                     ITTIQNGIDITRFKTLDSDKKKLLREKHGFDSEKRIFIYCGSLSLRKNIAYLLEHLAI
                     EKNDIFLILGDGELFRYCKDKYSKDLRYIFMGKVECPLEYYQLSDIFVSASLSEGLPL
                     ALLEAASTGCYLYVSDIEPHREIASLLGEENISMFKIKDGSYNYLQPKIKKADYNTLS
                     DDKLYNISDKKMSNLYDKLFVSLLEQRH"
     gene            7474..8247
                     /gene="wekZ"
     CDS             7474..8247
                     /gene="wekZ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WekZ"
                     /protein_id="ADC54969.1"
                     /db_xref="GI:288816249"
                     /translation="MIYVSVVSHGHFKILKELGAVTKLNNHSRIKVIIKDNLGESELL
                     DFCQENKITYLRSKEKKGFGENNNEVFSSISSLITKEDFFVVMNPDIYIECSDLLDVV
                     DECGSANVNLATINLYRDFDKKTYDNSVRKFPSAINFFMSFLFKKNDCVVNKNKITKP
                     TYVDWAAGSFLIFNAFFYSKLNGFNEKYFMYCEDIDICWRAKKHFNTSVLYYPCYAAI
                     HLAQFNNRRIFSRHFIWHIKSIILFLLYKNGMLRSSKLL"
     gene            8268..9698
                     /gene="manC"
     CDS             8268..9698
                     /gene="manC"
                     /note="mannose-1-phosphate guanyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="ADC54970.1"
                     /db_xref="GI:288816250"
                     /translation="MRMIPVILAGGSGSRLWPLSREKFPKQFLKLTGSLTMLQSTLSR
                     LNNLNADDSIVICNEEHRFIVAEQLRELGKLSNNIILEPKGRNTAPAITLAALAAKRK
                     FADEDPLILILAADHNIQDEHVFCEAINKASSLASYGKLVTFGIVPFKPETGYGYIRR
                     GDEVPADEQHAVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKY
                     RPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAGWS
                     DVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTKDA
                     VLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKP
                     GEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLSENESIYIPLGAMHCLENPGKIDL
                     ELIEVRSGAYLEEDDVIRCYDRYGRK"
     gene            9772..11151
                     /gene="manB"
     CDS             9772..11151
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="ADC54971.1"
                     /db_xref="GI:288816251"
                     /translation="MENMSSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTI
                     VLGGDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASH
                     NPMDYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVD
                     HLFGYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKVLGAPVELIKVHNTPDGNFPN
                     GIPNPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEA
                     FLEKNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSA
                     HHYFRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPVEA
                     INRVEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVQVMVIHT
                     QEILSILTS"
     gene            11272..12804
                     /gene="wzx"
     CDS             11272..12804
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADC54972.1"
                     /db_xref="GI:288816252"
                     /translation="MRTKKALHNFKVDLLITFLLVLLGFYIRTVFVSKMGSDVTGVML
                     LFTQLTAYLNLAELGIGIAAASVLYKPLSENEYNKITYIISLLSVIYKYIFVFVLILG
                     VVIGICIYYFIDSVKVVNGVFLYWALFVFNTSLTYSYAKYSTLLTANQRYSAVRKIQG
                     GGKVIIIVFQILILCFTQSFILYLLVETLGIFSQYLIFKKIIGNGNQYLSNEVLLIES
                     DKNLIKKELKIRIKNMFFHKIGAVLVLNTDYLLVSKFLTLSYVTIFGSYMMVFQIVTV
                     LMSSFVNAITAGMGNYLINKSNLEIKEITRQFYVIFIAFATFISLNMFFLVNDFIANW
                     IGVNYTLSNTLVALMIVNVFISVVRVPSDILKNASGHFGDIYYPLLEGVLNITISIIL
                     AIIIGLPGIIIGTIVSNLIVIMLAKPLYLYSKLFNLKNPMRVYFEFISRPMLYSLCVF
                     GVSYLLRDEIYSFKVNTWLDFINKLLLISTPSMLVICAIFSTDSDFRLFFRKIIYVVM
                     KK"
BASE COUNT     3923 a   2207 c   2786 g   4029 t
ORIGIN
        1 tgacgtactc agcctggtgc atgggggcgt attcagctga ctgatgccat tgccgaactg
       61 gcgaaaaaac agtccgttga tgcaatgctg atgactggtg acagctacga ctgcggtaaa
      121 aaaatgggct atatgcaggc gttcgtgaag tatgggctgc gcaacctcaa agaaggggcg
      181 aagttccgta aagggattga gaagctgtta agcgaataat gaaaatctga ccggatgtaa
      241 cggttgataa gaaaattata acggcagtga agattcgtgg cgaaagtaat ttgttgcgaa
      301 tattcctgcc gttgttttat ataaacaatc agaataacaa cgagttagca ataggatttt
      361 agtcaaagtt ttccaggatt ttccttgttt ccagagcgga ttggtaagac aattagcgtt
      421 tgaatttttc gggtttagcg cgagtgggta acgctcgtca catcgtaggc atgcatgcag
      481 tgctctggta gctgtaaagc caggggcggt agcgtgcatt aatacctcta ttaatcaaac
      541 tgagagccgc ttatttaaca gcatgctctg aagtaatatg gaataaaaga gtgaagatac
      601 ttgttactgg tggcgcagga tttattggtt ctgctgtagt tcgtcacatt ataaataata
      661 cgcaggatag tgttgttaat gtcgataaat taacgtacgc cggaaacctg gaatcacttg
      721 ctgatgtttc tgattctgaa cgctatattt ttgaacatgc ggatatttgc gatgctgctg
      781 caatggcacg gatttttgct cagcatcagc cggatgcagt gatgcacctg gcagctgaaa
      841 gccatgttga ccgttcaatt acaggtcctg cggcatttat tgaaaccaat attgttggta
      901 cttatgtcct tttggaagcc gctcgcaatt actggtctgc tcttgatagc gacaagaaaa
      961 atagcttccg ttttcatcat atttctactg acgaagtcta tggtgatttg cctcatccag
     1021 atgaagtaaa taataacgaa gaattaccct tatttactga gacgacagct tacgcaccaa
     1081 gcagccctta ttccgcatca aaagcatcca gcgatcattt agtccgcgcg tggaaacgta
     1141 cctatggttt accgaccatt gtgactaact gttcgaataa ctacggtcct tatcactttc
     1201 cggaaaaatt gattccacta gtaattctta atgctctgga aggtaaggca ttacctattt
     1261 atggcaaagg ggatcaaatt cgtgactggc tgtatgttga agatcatgcg cgtgcgttat
     1321 ataccgtcgt aaccgaaggt aaagcgggtg aaacttataa cattggtgga cacaacgaaa
     1381 agaaaaacat cgaagtagtg ctcactattt gtgatttgtt ggatgagatt gtaccgaaag
     1441 agaaatttta ttgtgagcaa attaattatg ttgccgaccg cccgggacat tattgccgtt
     1501 ttgcgattga tgctgagaag gttagccgcg aattgggttg gaaaccaccg gaaacgtttg
     1561 agagcgggat ttgtaaaaca gtggaatggt acctgtccaa tacaaaatgg gttgataatg
     1621 tgaaaagtgg tgcctatcca tcgtggattg aacagaaata tgagggccgc ccgtaatgaa
     1681 tatcctcctt ttcggcaaaa cagggcaggt aggttgggaa ataaagcgtg ctttggcacc
     1741 tctgggtaat ttgattgctc ttgatgttca ctcccctgat tactgtggtg attttagtaa
     1801 tcctgaaggt gtagctgaaa ccgtaagaag cattcggcct gatattattg tcaacgcagc
     1861 agctcacacc gcagtagaca aagcagaatc agaaccggag tttgcacaat tacttaacgc
     1921 gacgagtgtc gaagcgatcg cgaaagcagc caatgaagtc ggcgcatggg ttattcacta
     1981 ctctactgac tacgtatttc cggggaccgg tgaaatacca tggcaggagg cggatgcaac
     2041 cgcaccgcta aatgtttacg gtgaaaccaa gttagctgga gaaaaagcat tacaggaaca
     2101 ttgcgcgaag catcttattt tccgtaccag ctgggtatac gcaggtaaag gaaataactt
     2161 cgccaaaacg atgttgcgtc tggcaaaaga gcgcgaagaa ctggctgtga ttaatgatca
     2221 atttggtgcg ccaacaggtg ctgagctgct ggcagattgt acggcacatg caattcgtat
     2281 ggcactgaat aaaccagaag tcgcaggctt gtaccatctg gtagccagtg gtaccacaac
     2341 ctggcacgat tatgctgcgc tggtttttga agaggcacgc aaagcaggta ttccccttgc
     2401 actcaacaag ctcaacgcag taccaacaac agcttatcct acaccagctc gtcgtccaca
     2461 taactctcgc cttaatacag aaaaatttca gcagaacttt gcgcttgtct tgcctgactg
     2521 gcaggttggt gtgaaacgaa tgctcaacga attatttacg actacagcaa tttaatagtt
     2581 tttgcatctt gttcgtgatg gtggagcaag atgaattaaa aggaatgatg aaatgaaaac
     2641 gcgtaaaggt attattttag cgggtggttc tggtacacgt ctttatcctg tgactatggc
     2701 tgtcagtaaa cagctattac ctatttatga taagccgatg atctattacc cgctctctac
     2761 actgatgttg gcgggtattc gcgatattct gattattagt acgccacagg atactcctcg
     2821 ttttcaacaa ctgctgagtg acggtagcca gtgggggcta aatcttcagt acaaagtgca
     2881 accgactcca gatgggcttg cgcaggcgtt tattatcggt gaagagttta tcggtggtga
     2941 tgattgtgct ttggtacttg gtgataatat cttctacggt cacgacctgc ctaagttaat
     3001 ggatgccgct gttaacaaag aaagtggtgc aacggtattt gcctatcacg ttaatgatcc
     3061 tgaacgctac ggtgtcgttg agtttgataa aaacggtacg gcgatcagcc tggaagaaaa
     3121 accgctacaa ccaaaaagta attatgcggt aaccgggctt tatttctatg ataacgacgt
     3181 tgtcgaaatg gcgaaaaatc ttaagccttc tgcccgcggt gaactggaaa ttaccgatat
     3241 taaccgtatt tatatggaac aggggcgttt atccgttgcc atgatgggac gtggttatgc
     3301 atggctggac acggggacac atcaaagtct tattgaagca agcaacttca ttgcaacaat
     3361 tgaagagcgt caggggctga aagtttcctg cccggaagaa attgcttacc gtaaagggtt
     3421 tattgattct ggacaggtta gggtattagc cgagccactt aagaaaaatg cttatggtca
     3481 atatctgctg aaaatggtta aaggttatta ataaaatgaa tgtaattaaa actgaaattc
     3541 cggatgtatt aatatttgaa ccaaaagtat ttggggatga gcgtggtttc tttatggaaa
     3601 gctttaatca gaaagttttc gaagaggctg tagggcggaa agttgaattt gttcaggata
     3661 atcattctaa atcgtgtaaa ggtgtactta gaggtttaca ctttcagctt cctccctttg
     3721 agcaggcaaa attagtaagg tgtatagttg gcgaggtatt tgatgttgca gtagacatta
     3781 gacctaattc tgaaacattt ggttcatggg ttggagtaac tctttcgtca gaaaataaaa
     3841 ggcagctatg gattccagaa ggattcgccc atggtttttt aactttaagt gaaattgcag
     3901 agtttgttta taaaactaac aactattatt cttcaaatca tgaaagggga gtcatttgga
     3961 acgatgagga aattaacatt gcctggccct ctcaatcaga gaagattctg tcacagaaag
     4021 atattaattt accctcattt agatttgttc aaatgtttag caagtagtgt tatctttaca
     4081 ctgcacatag tcatcatttt ttatgcttta agtaaattat attgcacatc tataacacaa
     4141 agcgcaataa tatttcaacc tgatgaaggt ttgtgttatt tatctttcta ggcgtttttt
     4201 atgactaaaa tagttgtggt ttctacagct ccaatattcc cgacaaataa tgggtacaaa
     4261 agttctgtat taggaagaat tgatgagtta ttaaatgagg gtaatgaggt cgttttgatt
     4321 gaaataaacc ttgaaaaggt tacagaaaag aaagatgaat taataccaac aagatttaat
     4381 aatgttcaaa gatatgaagt aaaaaaaata tctagatcat ttattgccga gttacaaata
     4441 ttatttgata tcagaactcg gtatgaacaa ttattttctt ctgctgacat tagagataac
     4501 ataaaaaaaa taattgattt agaaaaacct tctattatta ttgctgagtc tatatgggcg
     4561 ttgcaagcct tgcctattga aattagtgcg agaatacact gtgtcattca tgatgtggca
     4621 attgatttct ttaaagaaat gtttgtatct cataatgagg ttgtacgaaa aattttgttt
     4681 tttaatgatt acctcaagtt gaaaattact gaagaaaata ttatcaaacg tttgagagtt
     4741 gagcaattta tctttctaac tgaagaagat aaatgttggt ataaaacaag atacaatatt
     4801 gatgagtgtt gttgttcctt agcgagcaat catctttatg tagaaaagat taagagaact
     4861 atcaatttcc aaacaccttt cctgcttatt cccggtagca ttgaattttc acaaaatttt
     4921 tatggcttaa attggtttat aaaaaatata tatcctagat taaataggga aataagaata
     4981 gttgtaacag gaaaggcatc agataaaaaa ataaagatgt taaactgtgg agaggaaatt
     5041 acctttacgg gagagcttga tttttacaca tataatcaac ttagctcaac atgcttgggt
     5101 gtcattgcac cgattacaac gggcactgga attaaaataa aaatattaga agctgtgcaa
     5161 aaaggtattc ctgtacttac aacaaaattt gcttcaaaag gaatatgttc cgatttatgt
     5221 ttttattgcg aagaggatac tgacacaaac tttgtcaatt taattaacag ttttcttgaa
     5281 actacattaa gagtccaaga atgaatttac tgcttttttc agtccttgca tttggtttaa
     5341 tattggcttt ggcccataat aataaaagtg gagatattaa cgcatactta atgttttttc
     5401 tcgtggtcct aatggtatta atctcagggc tgcgtatgaa tgatagcgat tatatcgaat
     5461 acaagaaaat gtataatgaa gtacctattt tatgtgactt tagtctcgca tctataagag
     5521 atatacatgg ggaggtaggc tatctattct tatcatcaat ctttaaaact ttatgcttgc
     5581 catttcaagt atttcttttt tttattgctt ttttatcact cctgcttaca tatttctcat
     5641 tcagaaagat aagtttaata ccgatactat cgttagtttt ttatttaagc catgctttta
     5701 tagttagaga tttgattcaa attagggcag gattagctgt tagcatatca ttatattcaa
     5761 taattaaatt taaaggaaat aaaagtataa ttacaggagt tttatttgct tctttgattc
     5821 attctggggc gcttattatt gctctttgct atcctttttt caaaaaaaaa tacataacat
     5881 taaaaatgat gttgttttta tttttagtgt cagttatttt ttcttatttg aatgggctta
     5941 acttatcgat acaactctta tctcagtata gtttgcttcc aactgcaatt tcgaattacg
     6001 ttggttggga agaatatgat tatcgggtga gtatatttac taatccggtt tttatcaaag
     6061 gtgttttttt aattgtctta atgcacaaat atgtactttc agatattaaa aatgagaaaa
     6121 ttatagtgct ttataactta tatgttttag gtgtattagc tatggttgca ttgagtggga
     6181 tggctattct ttcaggccgt ctttcatcct ttctgacact aggtgaaagc attttaattg
     6241 tatatgctct gttctacaaa agaaatacat ctttggcgtt tctaattttt tcttttttaa
     6301 caattgtgca attaggatat gatctcttta tttctaatgt gcatcctgag cttactctga
     6361 ttatatttgg gtgaatctaa gtgaaaaata ataaaatagg catacttatc tctaaaatac
     6421 aaaatcttgg acctgtgaac gtagtacgag gattgataaa agaaaataaa aaatatgctt
     6481 ttactgtttt ttgcttaaca aatagcgtag ataaaaatat atatgatgag ttatgctgtt
     6541 taggagccaa gattgtatta ataccagatg gtacttggtt cagcaaaatt ttatttgtga
     6601 gaaatttttt aaaggaacat ccacataata tcttacattc acatgggatc acggccgata
     6661 tgttttccta ctttctgaat ggcgtgaaaa tatctactat tcacaataga ctagatgagg
     6721 attatatccc attatttggc gcggttaaag ggaatgctat atattatctt catcgtttta
     6781 tattacgaag atttaatcat atcgttgctt gctcagcagc ggtccaatca aaactgaaac
     6841 aatcgaaagt aaaaactaaa ataaccacca tccagaatgg gattgatata actaggttta
     6901 agacacttga ctctgataaa aaaaaattat tgagggaaaa acacggattt gatagtgaaa
     6961 aaagaatatt tatatattgc ggctcgttat cattaaggaa aaatatcgct tatctcttgg
     7021 aacacttagc catcgaaaaa aatgatatat ttttaattct aggtgatggt gaacttttta
     7081 gatattgtaa ggataaatat tctaaagatt tacggtatat atttatgggg aaagttgaat
     7141 gccctcttga atattatcaa ctatcagata tttttgtttc cgcttctttg tcggagggac
     7201 tccccttggc actattagaa gctgcctcta ctgggtgcta tttatatgtt agcgatatag
     7261 agccccatag agaaatagca tctctattag gagaggaaaa tatttctatg tttaaaatta
     7321 aggatggctc atataattac ttgcaaccta aaataaaaaa agctgactat aacactcttt
     7381 ctgacgataa actttacaat atatccgata aaaaaatgtc aaatctttat gacaaacttt
     7441 ttgtttcttt attagagcag aggcactaat ataatgattt atgtttcggt agtttctcat
     7501 ggtcatttca aaatccttaa ggagttagga gcagtaacaa aattaaataa tcacagcaga
     7561 attaaagtta tcatcaaaga taatttagga gagagcgagc ttttggattt ttgtcaggaa
     7621 aacaaaataa cttatttaag gtctaaagag aaaaaaggat ttggagagaa taataatgaa
     7681 gttttttcct ctatatcttc cttaattact aaggaagatt tttttgtggt tatgaatcct
     7741 gatatatata ttgagtgctc tgatctatta gatgtcgtcg atgagtgtgg ttcagcgaat
     7801 gttaatctag caacgataaa tttatacagg gattttgata aaaaaacata tgataactca
     7861 gtaaggaaat ttccctcggc aattaatttt tttatgtcat ttttatttaa gaaaaatgac
     7921 tgtgtagtaa ataaaaacaa aataacgaaa ccaacatatg ttgattgggc tgcaggttct
     7981 tttctaatat ttaatgcctt cttttattca aaactcaacg gattcaacga aaagtacttt
     8041 atgtactgcg aagatattga tatatgttgg cgagctaaaa aacacttcaa tacttcagtt
     8101 ttatactatc catgctatgc agcaattcat ttggcacaat ttaacaatcg taggattttt
     8161 agtagacatt tcatttggca tataaaaagt attatccttt ttttattata taaaaatggt
     8221 atgctgcgtt ctagtaagtt gctttaatgc taatattctt ttaagaggtg agaatgatac
     8281 ctgttatttt ggctggtggt tcgggaagtc gcttgtggcc actttcacga gaaaagttcc
     8341 ccaagcagtt tttaaagttg actggcagtt tgacaatgtt gcagtcaaca ttgtcacgcc
     8401 ttaataattt aaatgctgat gattcaatag ttatatgcaa cgaagagcat agatttattg
     8461 ttgcagaaca attaagagag ttaggcaaac tttcaaataa cattattctt gaacccaaag
     8521 gtcgtaatac agcccctgct ataacactcg cagcattagc agcaaaaaga aaattcgctg
     8581 atgaagatcc attgattctt attttagctg cagatcacaa catccaagac gaacatgttt
     8641 tctgtgaggc aattaataag gcgtcatctt tagctagtta tggaaaacta gtgacttttg
     8701 gtatcgttcc attcaaacct gaaactgggt atggctatat tcgtcgcggt gatgaagtgc
     8761 ctgcagatga gcagcatgcg gtggcctttg aagtggcgca gtttgtcgaa aaaccaaatc
     8821 tggaaaccgc gcaggcctat gtggcaagtg gcgaatatta ctggaacagc ggcatgttcc
     8881 tgttccgtgc cggacgctat ctcgaagaat tgaaaaaata tcgcccggat atcctcgatg
     8941 cctgcgaaaa agcgatgagc gccgtcgatc cggatctcga ttttattcgt gtggatgaag
     9001 aagcgtttct ggcctgcccg gaagagtcgg tggattacgc ggtcatggaa cgcacggcag
     9061 atgccgttgt ggtgccgatg gatgcgggct ggagcgatgt cggttcctgg tcttcattat
     9121 gggagatcag cgcccacact gccgagggca acgtttgcca tggcgatgtg attaatcaca
     9181 aaactgaaaa cagctatgtg tacgccgaat ctggcctggt caccaccgtc ggggtgaaag
     9241 atttggtggt agtgcagacc aaagatgcgg tgctgattgc cgaccgtaac gcggtgcagg
     9301 atgtgaaaaa agtggtcgag cagatcaaag ccgatggtcg ccatgagcat cgggtacatc
     9361 gcgaagtgta tcgtccgtgg ggcaaatatg actctatcga cgcgggcgac cgctaccagg
     9421 tgaaacgcat caccgtgaaa cccggcgagg gcttgtcggt acagatgcac catcaccggg
     9481 cggaacactg ggtggtggtc gcgggaacgg caaaagtcac cattgatggt gatatcaaac
     9541 tgcttagtga aaacgagtcg atttatattc cgctgggggc gatgcactgc ctggaaaacc
     9601 cggggaaaat agatttagaa ttaattgaag ttcggtctgg tgcatatctt gaagaagatg
     9661 atgttattag atgttatgat cgctatggac gaaagtaata tataataatt atttcagaat
     9721 tagaaatgat tattataagt tttcatctgg ataaacaata gatagtatga gttggaaaat
     9781 atgagttctt taacttgttt taaggcttac gacattcgcg ggaaattagg cgaagaactg
     9841 aatgaagata tcgcctggcg tattggtcgc gcctatggcg aatttctcaa accgaaaacc
     9901 attgtgttag gcggtgatgt ccgcctcacc agcgaaacct taaaactggc gctggcgaaa
     9961 ggtttacagg atgcgggcgt cgatgtgctg gatattggca tgtccggcac cgaagagatc
    10021 tatttcgcca cgttccatct cggcgtggat ggcggcatcg aagttaccgc cagccataac
    10081 ccgatggatt acaacggcat gaaactggtg cgcgaagggg ctcgcccgat cagcggcgat
    10141 accggcctac gcgatgtcca gcgcctggca gaagccaacg acttccctcc cgtcgatgaa
    10201 accaaacgcg gtcgctatca gcaaatcaat ctgcgtgacg cttacgttga tcacctgttc
    10261 ggttatatca acgtcaaaaa cctcacgccg ctcaagctgg tgattaactc cgggaatggc
    10321 gcagcgggtc cggtggtgga cgccatcgaa gcccgcttta aagtcctcgg cgcacctgtg
    10381 gaattaatca aagtacacaa cacaccggac ggcaatttcc ccaacggtat tcctaacccg
    10441 ctgctgcctg aatgccgcga cgacacccgc aatgcggtca tcaaacacgg cgcggatatg
    10501 ggcattgcct ttgatggcga ttttgaccgc tgttttctgt ttgacgaaaa agggcagttt
    10561 atcgagggct actacattgt cggcctgctg gcagaagcgt tcctcgaaaa aaatcccggc
    10621 gcgaagatca tccacgatcc ccgtctctcc tggaacacca ttgatgtggt gacggccgca
    10681 ggcggcacgc cggtgatgtc gaaaaccgga cacgccttta ttaaagaacg tatgcgcaag
    10741 gaagacgcca tctacggtgg cgaaatgagc gcccaccatt acttccgtga tttcgcttac
    10801 tgcgacagcg gcatgatccc gtggctgctg gtagccgaac tggtgtgtct gaaaggaaaa
    10861 acgctgggcg aactggtgcg cgaccggatg gcggcgtttc cggcaagcgg tgagatcaac
    10921 agcaaactgg cgcaccccgt tgaggcgatt aaccgcgtgg aacagcattt tagccgtgag
    10981 gcgctggcgg tggatcgcac cgatggcatc agcatgacct ttgccgactg gcgctttaat
    11041 ctgcgctcct ccaacaccga accggtggtg cggttgaatg tggaatctcg cggtgatgtt
    11101 caggtcatgg taatccatac tcaagaaata ttatcaattt tgacgtcata aagaataagc
    11161 cctgacaagt tagggcttaa ttaatatata tttttttgaa ttgggaattt gtggaaagat
    11221 ttttaatatg ttatttaatg ttgttgaatt aatgttgact ggaaaataat aatgagaacg
    11281 aaaaaagcat tacacaactt taaagttgat ttattaatta cttttttatt ggttttgcta
    11341 gggttttata ttcgaactgt ttttgtttca aaaatgggaa gtgatgttac tggagtgatg
    11401 ttactattca cacagttgac agcgtatctc aatttggcag aattaggtat tggcattgca
    11461 gctgccagcg tattatataa accgctcagc gagaatgaat acaataaaat aacttacata
    11521 atatctttgc tctcagttat atacaaatat atatttgtgt ttgttttgat tcttggcgtt
    11581 gttataggta tctgtattta ttactttatt gattctgtaa aggttgtaaa cggcgttttt
    11641 ttatattggg ctttgttcgt ttttaataca tcgttgacat atagttatgc taaatactcc
    11701 acattattaa ctgctaatca gcggtactca gcagtaagaa aaattcaagg tggcggaaaa
    11761 gttataataa ttgtatttca gatattaatt ttgtgcttta cgcaaagttt catactttat
    11821 ttgttagttg agactttagg tattttttct caatatttaa tttttaaaaa aataattggg
    11881 aacggaaatc aatatctcag taatgaggtt ttacttattg aaagcgataa aaatttgata
    11941 aaaaaagaat taaaaataag aatcaaaaat atgttcttcc ataaaatagg cgctgtgctg
    12001 gtccttaata cagactacct gcttgtatca aagtttctga cattaagtta tgtgacaatt
    12061 tttggcagct atatgatggt atttcagata gtaactgttt tgatgtcaag ttttgttaat
    12121 gctattactg caggaatggg taattactta attaacaaaa gtaatttaga aattaaggaa
    12181 attacacgtc aattttatgt gatatttatc gcctttgcaa cattcatatc actaaatatg
    12241 ttttttcttg ttaatgattt tatcgcaaat tggataggtg ttaattatac attaagtaac
    12301 accctagttg cattaatgat tgttaacgta ttcattagtg ttgtcagggt accttctgat
    12361 atattaaaaa acgcaagtgg acattttggc gatatttatt atccattatt agaaggtgtg
    12421 ttgaatatta cgatatctat cattttggct atcattattg gattgcctgg cattattata
    12481 gggacaattg tatctaactt aatagtaata atgctcgcga aacctttata tctttactct
    12541 aagttattta atcttaaaaa tccgatgagg gtttattttg aatttatttc tcggcctatg
    12601 ttatattcat tatgtgtgtt tggggtaagc tatttattgc gcgatgaaat atattcattt
    12661 aaagtaaata catggttgga ttttattaac aaactactct taatctctac tcctagtatg
    12721 ttggtaatat gtgctatttt ctctacggat agtgacttta gattattttt cagaaaaatt
    12781 atatatgtgg ttatgaagaa ataaaaattt cgaaaatgta ttaatcgaaa ttatgcaaca
    12841 agctttattt ttataaatga tatgtgatct tttcgcgaat aggagtaaac aatgtcaaag
    12901 caacagatcg gcgtcgtcgg tatggcagtg atggggcgca accct
//
