LOCUS       AB812031               15443 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: E5d.
ACCESSION   AB812031
VERSION     AB812031.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 15443)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..15443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E5d"
                     /db_xref="taxon:562"
                     /note="serogroup:O76"
     gene            <1..905
                     /gene="wcaM"
     CDS             <1..905
                     /gene="wcaM"
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01212.1"
                     /translation="YAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILIS
                     DHVIERINCTNDKINWGIGIGLSGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVEN
                     GKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIK
                     GKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKP
                     QHFFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSV
                     DIDRINHHIVNVEKINFRLPERRE"
     gene            1063..2058
                     /gene="gne"
     CDS             1063..2058
                     /gene="gnu"
                     /note="O076_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAQ01213.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            2301..3200
                     /gene="galF"
     CDS             2301..3200
                     /gene="galF"
                     /note="O076_02"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01214.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRLFIQNLVTRSN"
     CDS             3289..4287
                     /note="O076_03"
                     /gene="wbsL"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01215.1"
                     /translation="MNQVQYTGSCEARGGSVVFYEHHDDMIVINFIFPVHENLPEIKH
                     YIAYFSMHDIVCLNNALSIPKFDKDIEKYVEWHIMGTHFYNQPRFLNQVKGRSYLIHE
                     YASLSTGKNKFIKLFKDILKHNCSHRPDYQLFLNKYIQSKMSVRGIPGELRDMGVCQE
                     FIKNRHHSSNKTVKHYDFVYVGSMAAERKIEHFLDSYIMRQKKILLIGSPSEYLVKRY
                     KKKDNIHFIGRVEQSEIPNLIANVKVCINYIPDVYPYNRQTSTKLIEYLTLGKDVISN
                     KYKWVMDFVNDNNLEYESIDNNFIYIHNAGDYIAQDWHSVIDNLNITRMLKNNESF"
     gene            4274..5512
                     /gene="wzx"
     CDS             4274..5512
                     /gene="wzx"
                     /note="O076_04"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01216.1"
                     /translation="MNHFKNTILFSIERLLRTGLGFVVSIMITKAYGVEVYGEYAYYF
                     AIINILGVALSLGLDDYFVKCAAESHKVPIKLLYIRIALNSLSFIFCGFVLYWKKAGV
                     EFWLLSLLSLTYIWNCFYLLLIVLDRPNKHKSILTLITINLLFLILKFLLINYGFIVF
                     IVITLIETLTVTVFLYISIRCVFHIEDINLYRIKGVFKISIPVGISSLSIMLFYRLDQ
                     MIVEHYMGVKALGIYALSASMILAAGYLQSAYVTGMYSSIGAAKNNTNQRDMHKVLLK
                     AYRGAICIGIIVYIGYITVGRIIIKHIFNEISFDLISLLDIGMISILFSGLAAINSQY
                     LFVQGYANKRLLRTLICLLFNISWNIILIPKFGIMSAVWGYLITQIIMGVLFNVFDKV
                     TRQLFILQFKSLFIYRVNKT"
     CDS             5509..6078
                     /note="O076_05"
                     /gene="wcdG"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="BAQ01217.1"
                     /translation="MKKYILAFVKYVFSPVCFFMYFLYVIKIINYKTSTQILAFVPGT
                     IGLLIRQFYYELTLTSCGKNLRVFWGAYIVYPDVMIGDNCTIEEFCIISKCRLGNDVI
                     LAARVSIMSGSKHHDINDITKTFGESKAEYRTIHLGNNLWIGTHAVIMNDIGDHTAVG
                     AGSVVTRAIPEMVVAAGVPARIIKHRGKL"
     gene            6078..7310
                     /gene="wzy"
     CDS             6078..7310
                     /gene="wzy"
                     /note="O076_06"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01218.1"
                     /translation="MSEVFSKIYIFIILVVSPVLTLFSLFGLFPFYPVNFFIIGFGFF
                     ILIGFLFKAFYIIRINISINDCLIASSFTWFFIIILCYSDLYSYTEDGRYISAFRYYM
                     PFFIISLCCYFIFKKGCAFFIKPIGLYSLLLMYCALVVITFLYFNLGTFKIDFSQLID
                     PSFIGVYQIVSDSLAFMAICVLSLSNLSKFSRISVIVITLFFLLILNARSGLIGFLLA
                     LCFIFDIRKIFIEKPVYVFFSMLLLIVIFMHISPDFQHISSFFESSNSRIYNIFSGNF
                     YDDASFVGRMKLLLHSLSIIAAHPILGSFGSQSETDIDGFGVRWGAYTHNIFVYWDQF
                     GIIGFALVSLIICMCYYNNKLLKRVSGIDLFALIVLVLSQQLFLKSFTYFYLFAMAGL
                     IEGMKNVNKHNYNYQKFK"
     CDS             7270..8169
                     /note="O076_07"
                     /gene="wbhR"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01219.1"
                     /translation="MLISIIITTRNSSSYIKKTLDAIVSQTFQSYEVLVIDDASDDIS
                     ELTKIIENYSAIITIRVIKQSTKTNASVTRNIGIKNSIGEIICFLDADDIWRENKLEV
                     VAKVFDDFKNESTIVFHQSLRGTLNDINAGKGKIVPETGPATNNIVDYLMNEHGVIQT
                     STISINRKSAEILVFDESLPRHQDIQFCFDAYKNKVNFIFVEQILSNWIILDRASNAH
                     TKGANVDFCLGWINKNRSVLTDKNIVNYLSNVLFLIAIKEKAFFRTIPISVALLGCNS
                     LIAWWKFFILVFSRVLKVFKRKS"
     CDS             8195..9232
                     /note="O076_08"
                     /gene="wbwH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01220.1"
                     /translation="MSKKLVYIITKTETGGAQKWIKEQKQLLSKCFDIYLITSEPGWL
                     SEQFTEDHVFYVKDILSFKSIFASYKIAKILRKLNASVVINNSANAGLHGRLAKLYYY
                     HRSIYVSHGWSCIYNGGRLSKIFCLIERILAVLTDKILCVSKRDFEKANQQIGISEKK
                     LVTITNGIMPLRPRTCDRLNGELNLVFVGRMIPPKRPDLLLYVVSQFSNVKIDMIGDG
                     PLLEGLRHKYGSFKNIRFLGEIKNFDGFYKYDAFVLTSDSEGLPMSALEAASAGLPML
                     LSDVGGCSELIDNLNPNGILYNNEIESICEAIKKLIRDYPSYELFAKQQKERFSLLSK
                     YDEYLNLINGK"
     CDS             9276..10298
                     /note="O076_09"
                     /gene="gne"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-glucose 4-epimerase"
                     /protein_id="BAQ01221.1"
                     /translation="MKVLVTGGAGYIGSHTVLELLSRGDDVVVIDNLVNSSRTSLDRV
                     FELCGRAPVFYEGDVRDKELLKTIFTEHDIDSIIHFAGLKSVSESIRKPLEYYENNIV
                     ATISLIDEAAKYGVMNFIFSSSATVYGNPDFVPISESSKIGGTTNPYGTSKLFAEQIL
                     KDFAACNVNFNVTILRYFNPIGAHPSGRIGENPNGIPNNLVPFVSQTAVGKHKVVSIY
                     GNDYPTLDGTGIRDYIHVTDLAKGHLAALDHIGKHGNYNVYNLGTGQGYSVLDVINTF
                     QKISGIAINYIFSQRRSGDIAECWSDPSLALKELQWQAKLSLEDMLRDAWNWQKNNPD
                     GYNIHD"
     gene            10461..11867
                     /gene="gnd"
     CDS             10461..11867
                     /gene="gnd"
                     /note="O076_10"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01222.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVSYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEEGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            12116..13282
                     /gene="ugd"
     CDS             12116..13282
                     /gene="ugd"
                     /note="O076_11"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01223.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            13372..14406
                     /gene="wzz"
     CDS             13372..14406
                     /gene="wzz"
                     /note="O076_12"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01224.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGS
                     AAPKVSEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAQYIQQVDDQVNEELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(14503..15114)
                     /gene="hisI"
     CDS             complement(14503..15114)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01225.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSDNFLNVVSIVPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTVHQWLFLYQLEQLLAERKSADPKTSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     4555 a   2831 c   3294 g   4763 t
ORIGIN
        1 attacgccat cttacgccag ggatttcata accagattat cggtgccaac atcaccaatt
       61 gtaagttcag cgacttacag ggggacgcta ttgaatggaa cgtggcgatt aacgacagtg
      121 atattttgat ctccgaccat gtcatcgagc gcatcaactg tactaacgac aaaatcaact
      181 ggggcatcgg cataggtctt tcgggtagta cctacgataa caactacccg gaagaccagg
      241 cagtgaaaaa ctttgtcgtg gcgaatatca cgggatcgga ttgtcggcag ttgatccatg
      301 ttgaaaatgg taaacatttt gttattcgta atatcaaagc ccgcaatatc acaccggatt
      361 tcagtaagaa agcaggtatt gataacgcca cagtcgctat ttacggttgt gacaatttcg
      421 tgattgataa tattgaaatg attaatagcg ccgggatgtt aatcggctat ggggtaatta
      481 aaggcaaata tctctcgata ccgcaaaatt tccgagtgaa taatattcaa ctggataata
      541 cccaccttgc ttataaattg cgcggtatcc aaatctccgc cgggaatgcc gtctcctttg
      601 tgtcgctaac taacattgag atgaagcgtg cctcgcttga gcttcacaat aaaccgcaac
      661 acttttttat gcgaaatatc aatgtgatgc aggaatcctc tgttggacca gcattgagca
      721 tgaacttcga catgcgcaaa gacgtacgag gtgtctttat ggcgaaaaaa gaaacactcc
      781 tgtctcttgc aaatgttcat gcggtgaatg aaagaggaca aagctccgtc gatatcgaca
      841 ggattaatca ccatattgtt aatgtggaaa agattaactt tagattgccg gaacggaggg
      901 agtagatttg cgaccattca taaacttact ggatataatg ttctaaaaga ttataatcct
      961 cacactaact gttaaagctg gggctgggca gcattgacta ctcagggaag gtactcatct
     1021 ttatcttgtg tttcttacgt aacaaccaga ctaaagtgat ttatgaacga taacgttttg
     1081 ctcataggag cttccggatt cgtaggaacc cgactacttg aaacggcaat tgctgacttt
     1141 aatatcaaga acctggacaa acagcagagc cacttttatc cagaaatcac acagattggc
     1201 gatgttcgtg atcaacaggc actcgaccag gcgttagccg gttttgacac tgttgtgcta
     1261 ctggcagcgg aacaccgcga tgacgtcagc cctacttctc tctattatga tgtcaacgtt
     1321 cagggtaccc gcaatgtgct ggcggccatg gaaaaaaatg gcgttaaaaa tatcatcttt
     1381 accagttccg ttgctgttta tggtttgaac aaacacaacc ctgacgaaaa ccatccacac
     1441 gatcctttca accactacgg caaaagtaag tggcaggcag aggaagtgct gcgtgaatgg
     1501 tataacaaag caccaacaga acgttcatta accatcatcc gtcctaccgt tatcttcggt
     1561 gaacgcaacc gcggtaacgt ctataacttg ctgaaacaga tcgctggcgg caagtttatg
     1621 atggtgggcg cagggactaa ctataagtcc atggcttatg ttggaaacat tgttgagttt
     1681 atcaagtaca aactgaagaa tgttgccgca ggttatgagg tttataacta tgttgataag
     1741 ccagacctaa acatgaacca gttggttgct gaagttgaac aaagcctgaa caaaaagatc
     1801 ccttctatgc acttgcctta cccactagga atgctgggtg gatattgttt tgatatcctg
     1861 agcaaaatta cgggcaaaaa atacgctgtc agctcagtgc gcgtgaaaaa attctgcgca
     1921 acaacacagt ttgacgcaac gaaagtgcat tcttcaggtt ttgtggcacc gtatacgctg
     1981 tcgcaaggtc tggatcgaac tctgcagtat gaattcgtcc atgccaaaaa agacgacata
     2041 acgtttgttt ctgagtaatg cagacgagtc agagaaaaaa ggaccaggtt ggactacgat
     2101 tattcggaaa gaggtactga ttaaattatc agtatgcgga attccgagta atccgagtaa
     2161 cccagatgct gatggtgtgt agtgttatcc taaggtttaa gcaaagttat gcggaactaa
     2221 gctttggtat atagccataa cgtttggcta acctatggca ataatttacg cactatcagc
     2281 cacttagagg taggaaaaga atgacgaatt taaaagcagt tataccggta gcaggtcttg
     2341 ggatgcatat gttgcctgcc actaaggcga ttcccaaaga gatgctaccg atcgtcgaca
     2401 agccaatgat tcagtacatt gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc
     2461 tggtaactca cgcgtccaag aacgcagtcg aaaaccactt cgacacctct tatgaattag
     2521 aatctctcct tgaacagcgc gtgaagcgtc aactgctggc ggaagtacaa tctatctgtc
     2581 cgccgggcgt gactattatg aacgtgcgtc agggtgaacc tttaggttta ggccattcca
     2641 ttttgtgtgc acgacccgcc attggtgaca acccatttgt cgtggtgctg ccagacgttg
     2701 tgatcgacga cgccagtgcc gatccgctgc gttacaacct tgcagccatg attgcgcgtt
     2761 tcaatgaaac gggccgtagc caggtgctgg caaaacgtat gccgggtgac ctctctgaat
     2821 actctgttat tcagaccaaa gaaccactgg atcgtgaagg taaagtcagc cgcattgttg
     2881 aatttatcga aaaaccggat cagccgcaga cgctggactc agacatcatg gccgttggtc
     2941 gctatgtgct ttctgctgat atttggccgg aactagaacg cactcagcca ggtgcatggg
     3001 gacgtatcca actgactgat gccattgctg aactggcgaa gaaacagtcc gttgatgcca
     3061 tgctgatgac tggtgacagc tacgactgcg gtaaaaaaat gggctatatg caggcgtttg
     3121 tgaagtatgg actacgcaac ctgaaagaag gggcgaagtt ccgtctcttt attcagaatt
     3181 tagtgacaag aagtaattag taaaaatagt gacaattcga tggcgaatat taagccatcg
     3241 aattttgaaa ggttataata tttaaatcat ccatatataa actctggatt gaatcaggtg
     3301 cagtatactg gtagctgtga agccaggggc ggtagcgttg ttttttatga gcatcatgat
     3361 gatatgattg taataaattt tatattccca gtacacgaaa atttaccaga aataaaacat
     3421 tatatcgctt atttttctat gcacgatatt gtttgtttga acaatgcact atccatacca
     3481 aaatttgata aagatataga aaaatatgtt gaatggcata tcatggggac acacttttac
     3541 aatcaaccac gttttttgaa tcaagttaaa ggccgtagct atttgattca tgagtatgca
     3601 tctttatcaa ccggaaaaaa caagtttata aagttattta aagatatact taaacataat
     3661 tgcagtcatc gtccagatta ccaattattt cttaataaat atatacagtc aaaaatgtca
     3721 gtgcgtggaa tccctggtga attaagggat atgggggttt gtcaagagtt tataaaaaat
     3781 cgccaccata gctctaataa aactgttaag cactatgatt ttgtatatgt tggtagtatg
     3841 gcagcagaac gaaaaataga gcattttctt gattcatata ttatgaggca gaaaaaaata
     3901 ttgcttattg gttcgccatc agaatattta gtgaaaagat acaaaaaaaa agacaatata
     3961 cattttattg gtagggtgga acaaagtgaa atacctaatt taattgcaaa tgttaaagta
     4021 tgtataaatt atattcctga tgtgtatcct tataatagac aaacatcgac aaagctaatt
     4081 gaatatttaa ctttaggaaa ggatgtcatt agtaataaat ataaatgggt tatggatttc
     4141 gtaaatgata ataatctgga gtatgaatcg attgataata actttatcta tattcataat
     4201 gcaggggact atattgcgca agattggcat tcagttatcg ataatctaaa tataacaagg
     4261 atgttaaaga ataatgaatc attttaaaaa tacaatactc tttagcattg aacgattatt
     4321 gcgtacaggc cttggtttcg tcgtttcaat tatgattaca aaagcatacg gcgtcgaagt
     4381 ctacggtgaa tatgcttatt attttgcgat catcaatata cttggagtcg cattatcatt
     4441 aggattagat gattatttcg taaagtgtgc tgctgaaagc cataaagtgc caataaaatt
     4501 actttatata cgaattgcac tgaatagtct ttcgttcata ttttgtggtt ttgttttgta
     4561 ttggaaaaaa gcaggagttg agttttggct tctatctctt ttatctctaa catatatatg
     4621 gaattgtttt tatttgcttc tgattgtatt ggatcgacca aataaacata aatcaatatt
     4681 gactctgatt actattaatt tgctcttctt aatattaaag tttttactta taaactatgg
     4741 atttatcgta tttatcgtaa ttacactgat tgaaactctt acggtaacag tttttttata
     4801 tatatctata aggtgtgtat ttcatataga ggatataaat ctatatagga taaagggtgt
     4861 ttttaaaata tctattcctg ttggtatctc atctctctct ataatgttat tttatcgtct
     4921 ggatcagatg atagtagaac actatatggg agtaaaagca ttaggtattt atgccttatc
     4981 cgcaagtatg attcttgccg caggctacct tcaatctgca tatgtaacgg gaatgtactc
     5041 atcaattggg gccgctaaaa ataatacaaa tcagagagac atgcataaag tattattaaa
     5101 agcctatcgt ggagcaattt gtattggcat aatagtttat atcgggtata taacggtcgg
     5161 tcgcattatt attaaacata tattcaatga aatatcattc gatttgatat cgctactgga
     5221 tatcggaatg atttctattt tattttcagg tcttgctgct atcaattcac aatatttatt
     5281 tgttcaaggt tatgcaaata aaagattact aagaactctt atttgtcttt tatttaatat
     5341 ttcgtggaat attattctga ttccaaaatt tggtatcatg tctgcagtat ggggatatct
     5401 tattacacag attatcatgg gcgtactatt taatgttttt gataaggtaa ctagacagtt
     5461 atttatttta caatttaaat cattgtttat atacagagta aataagacat gaaaaaatac
     5521 attttagcat ttgtcaagta tgttttttct cctgtttgtt tttttatgta ttttttatat
     5581 gtgattaaaa ttataaatta taagacatcc acacaaatat tagcttttgt gcctggaact
     5641 attggtctgt tgattaggca gttttactat gaactgacct taacatcttg tgggaaaaat
     5701 ttaagagtgt tttggggagc atatattgtt tatcctgacg ttatgattgg ggataattgt
     5761 acgattgagg agttttgtat tataagcaag tgtaggctag gaaatgatgt tatacttgca
     5821 gctagggtat caataatgag cggttcgaaa catcatgata taaatgatat cactaaaact
     5881 tttggtgaaa gcaaagctga gtatcgcaca atccatttag gaaacaacct atggattggt
     5941 acacacgctg tcattatgaa tgatattggc gatcacacgg ctgtaggtgc cggatctgtt
     6001 gtaacgaggg cgattcctga aatggttgtt gctgctggtg ttcctgctcg aataataaaa
     6061 catcgtggga agctataatg tcagaagtat tttcaaaaat atatatattt atcatacttg
     6121 tcgtttctcc agttttaact ctgttttctc tctttgggtt atttcctttc tatcctgtta
     6181 atttttttat tattgggttt ggttttttta tattaatagg ctttttattc aaggcgtttt
     6241 atataatacg tattaacata tcgattaatg attgcttaat tgcatcttca tttacatggt
     6301 tttttataat aatattatgc tatagtgatc tctactcata tactgaagat ggtcgttata
     6361 tttctgcttt tagatactat atgccatttt tcataatatc attatgttgt tattttatat
     6421 tcaaaaaagg gtgcgcattt tttataaaac caataggatt atactcctta ttgctgatgt
     6481 attgtgcatt agttgttata acttttttat attttaatct tggaacattt aaaatagatt
     6541 tttcacagtt aatcgatcct tcatttattg gagtctacca aatagtaagt gacagcttgg
     6601 cttttatggc gatatgtgta ttgtcattaa gtaatctatc aaaattttct aggatatcgg
     6661 tcattgttat cacgttattt ttcttgctta tacttaacgc tcgttcagga ttaataggtt
     6721 ttcttttagc tttatgtttc atttttgata taagaaaaat ttttatagaa aaaccagttt
     6781 atgtcttttt tagtatgttg ttactgatag tcatatttat gcatatttct cctgactttc
     6841 agcatatttc ctcatttttt gaaagctcta atagcaggat atataatata ttctcaggga
     6901 acttttatga tgatgcttct tttgtgggta gaatgaagtt acttttacat tccttatcta
     6961 ttattgcagc acatccaatt ttaggatctt tcggttcaca atctgaaact gatattgatg
     7021 ggttcggtgt ccgttggggg gcttatactc acaacatatt tgtttactgg gatcagtttg
     7081 gtataattgg ttttgcatta gtcagcttaa ttatatgcat gtgttactat aataacaaac
     7141 tcttaaaaag agtttcggga attgatttat ttgcattgat tgttctggta ttatctcaac
     7201 agctttttct taaaagcttt acttatttct atttgttcgc catggctgga cttattgaag
     7261 gtatgaaaaa tgttaataag cataattata actaccagaa attcaagtag ttatatcaaa
     7321 aaaactcttg atgcgattgt atcgcagaca tttcagtcat atgaagtatt agtaatagat
     7381 gatgctagcg atgatattag tgagctgact aaaatcattg aaaactattc agcaattatt
     7441 acaattcgag ttattaagca gagcactaaa acgaatgcct ctgtcaccag aaatatagga
     7501 atcaaaaact caattggtga aataatttgt ttccttgatg ctgatgatat ctggagagaa
     7561 aataagcttg aggtggttgc taaggttttt gacgatttca aaaacgaatc gacgattgtt
     7621 tttcatcaat cattgagagg taccctcaat gatattaatg ctggaaaagg aaaaattgta
     7681 cctgagacag gtcctgccac caacaatatc gttgattatt tgatgaacga acatggtgtt
     7741 atccagactt caactattag tataaataga aagtctgctg aaattttggt atttgacgaa
     7801 tcattgccga ggcatcaaga tattcaattt tgttttgatg catataagaa taaggttaat
     7861 ttcatttttg tggagcagat attatctaat tggataattt tagatagagc atcaaatgct
     7921 catactaaag gcgcaaatgt tgatttttgc ttaggttgga ttaataaaaa caggtctgtt
     7981 ttaactgata aaaacatcgt taactatctt agcaatgttt tatttttgat cgctataaag
     8041 gagaaagctt ttttcagaac gataccaatc agtgttgctt tattaggctg caattcattg
     8101 attgcatggt ggaaattttt tattcttgtt ttttctagag ttttaaaggt atttaaaaga
     8161 aaaagctaaa ttgatgattt aaagagtaaa atctatgtca aaaaaattag tatatattat
     8221 tacaaaaaca gaaacaggcg gtgctcagaa atggattaaa gaacaaaagc aactgctatc
     8281 aaagtgtttt gacatttatc ttattacttc cgaaccgggg tggttatccg aacaatttac
     8341 agaggatcat gtcttttatg ttaaagatat tttatcgttt aaatcaatat ttgcgtctta
     8401 taagattgca aaaattttaa gaaaattaaa tgcatcagta gtaataaata attcagcaaa
     8461 tgcaggtctg catggacgac ttgcaaagct ttattattat catcgttcaa tctacgtatc
     8521 tcatgggtgg tcttgcattt ataatggcgg gagactatcg aagatttttt gtttgataga
     8581 aagaatttta gctgtattaa ctgataagat tctttgtgta tcaaaaaggg actttgaaaa
     8641 agcaaatcag cagataggta tttcagaaaa gaaattagtt actataacta atggcattat
     8701 gccgttacgc cctagaacct gtgaccgctt gaatggtgag ttgaatttag tttttgttgg
     8761 gcgaatgata cctccaaaaa gaccagattt attactttat gtcgtatctc agttttcaaa
     8821 tgtgaaaata gatatgatag gtgatgggcc attgttggaa ggccttaggc ataaatatgg
     8881 ttcttttaag aatataagat ttttaggaga gattaaaaat tttgacggtt tctataagta
     8941 tgacgcattt gttttaacct cagatagcga aggtttacct atgtctgcgt tagaagctgc
     9001 atcggctggt ttaccaatgt tgctaagtga tgttggtggc tgtagtgagt taattgataa
     9061 tctaaaccct aatggcattt tgtacaataa tgagattgaa tctatatgcg aagcaataaa
     9121 aaagctgatt agagattatc ctagctatga actctttgct aaacaacaaa aagaacgctt
     9181 tagtttactt agtaaatatg atgagtatct taatctcata aatggaaagt aagatttttc
     9241 tttaggtatt ttactgaatt gatggtgaga gaaatatgaa agtacttgtt actggtggcg
     9301 caggttatat aggttctcat actgtactgg aactattaag tcgtggtgat gacgttgttg
     9361 taatcgataa tcttgttaat tcatctcgta cttcattgga tagagtattt gagttgtgtg
     9421 ggcgtgcacc tgtattctat gaaggagatg ttcgagataa ggagctttta aagacaatat
     9481 ttaccgaaca cgatattgat tcaatcattc actttgctgg gttaaaatca gtctcggagt
     9541 caatacgtaa acctcttgag tattatgaaa ataacattgt agcaacaata tctttgattg
     9601 atgaagcagc aaagtatgga gtcatgaatt ttatttttag ctcatcagct acagtatatg
     9661 gaaatcctga ttttgttcca atatctgaat caagtaaaat tggtggtaca acaaatccat
     9721 atgggacgtc aaaattattt gctgagcaaa tactgaaaga ttttgctgca tgtaatgtaa
     9781 attttaatgt gacaatattg aggtatttca accctatagg tgctcatcct agtggaagaa
     9841 taggcgagaa tcctaatggt atacctaata atctggttcc ctttgtttca cagactgctg
     9901 ttggaaaaca taaagtagtt tctatatatg gaaatgacta cccaactttg gatggtactg
     9961 gtattagaga ttatattcac gttactgatc ttgctaaagg acatttagca gctttagatc
    10021 atattggtaa acatgggaat tacaatgtct acaatttagg tactggacag ggatattctg
    10081 ttcttgatgt cattaataca tttcaaaaaa tatccggaat agcaattaat tacatatttt
    10141 ctcaacgaag aagtggtgat attgctgagt gttggtctga cccttcttta gcattaaagg
    10201 agttacagtg gcaggcgaaa ctttctttag aggatatgtt gcgagatgct tggaattggc
    10261 aaaaaaataa tcctgatggt tataatatac atgattaact tttcacatct acgaaaatga
    10321 tcaattagaa tatatatgac acggctttaa agtcattaat atacgtctac taccaaatgt
    10381 gttattactg agaaacacat gagacgattt ataatcgcgt ataaattatg cggtggctac
    10441 ccccctgaca ggagtaaata atgtcaaagc aacagatcgg cgtcgtcggt atggcagtga
    10501 tggggcgcaa ccttgcgctc aacatcgaaa gtcgtggtta taccgtctct attttcaacc
    10561 gttcccgtga aaagacggaa gaagtgattg ccgaaaatcc aggcaagaaa ctagtttctt
    10621 actatacggt gaaagagttt gttgaatctc tggaaacgcc tcgtcgcatc ctgttaatgg
    10681 tgaaagcagg tgcaggcacg gatgctgcta ttgattctct caagccatac ctcgataaag
    10741 gcgacatcat cattgatggt ggtaatacct tcttccagga caccattcgt cgtaaccgcg
    10801 agctttctgc cgaaggcttt aactttatcg gtaccggtgt ttccggtggt gaagaaggtg
    10861 cgctgaaagg tccttccatt atgcctggtg ggcagaaaga agcctatgag ctggttgcac
    10921 caatcctgac caaaatcgcc gcagtagctg aagacggtga gccatgcgtt acctatattg
    10981 gtgctgatgg tgcaggtcac tatgtgaaga tggttcacaa cggtattgaa tacggcgata
    11041 tgcagctgat tgctgaagcc tattctctgc ttaaaggtgg cttgaacctt tccaacgaag
    11101 aactggcgca gacctttacc gagtggaata acggtgaact gagcagctat ctgatcgaca
    11161 tcaccaaaga tatcttcacc aaaaaagatg aagagggtaa ctacctggtt gatgtgattc
    11221 tggatgaagc ggctaacaaa ggtaccggta aatggaccag ccagagcgcg ctggatctcg
    11281 gtgaaccgct gtcgctgatt accgagtctg tgtttgcacg ttatatctct tctctgaaag
    11341 atcagcgcgt tgccgcatct aaagttctct ctggcccgca agcgcagcca gcaggcgata
    11401 aggctgagtt tatcgaaaaa gttcgtcgtg cgctgtatct gggcaaaatc gtttcttacg
    11461 ctcagggctt ctctcagctg cgtgcggcgt ctgaagaata caactgggat ctgaactacg
    11521 gcgaaatcgc gaagattttc cgtgctggct gcatcatccg tgcgcagttc ctgcagaaaa
    11581 tcaccgatgc ctatgccgaa aatccgcaga tcgctaacct gctgctggcc ccgtacttca
    11641 agcaaatcgc cgatgactac cagcaggcgc tgcgcgatgt cgtcgcttac gcagtacaga
    11701 acggtatccc ggttccgacc ttcgccgctg cggttgccta ttatgacagc taccgcgccg
    11761 cagttctgcc tgcgaacctg atccaggcac agcgtgacta tttcggtgcg catacttata
    11821 agcgcattga taaagaaggt gtgttccata ccgaatggct ggattaatct gatttaaatc
    11881 aattaatcaa tgcaaggccc ggagaaaccc tccgggcttt tttattatac aaagcggcag
    11941 gttatggcct ttttttataa tttatggtta aaaaagcgat ataatacagc gccgcacagc
    12001 aggatcgctg ccttgacagt tcatctacat cagcgttaaa aatcccgcag tagatgaagt
    12061 tgtggtggtg gattaatgac cactctaaat gtttaaccgg aagaagtcag agctaatgaa
    12121 aataacaatt tcaggaacag gttatgttgg tctttcaaat ggtattctga ttgcgcaaaa
    12181 tcacgaagtg gttgcactgg atatcgttca ggccaaagtg gacatgctta acaagaagca
    12241 gtcaccgatt gttgataagg agattgaaga gtatctggcg actaaagatc tcaatttccg
    12301 cgctacgaca gataagtatg acgcgtataa aaatgccgat tacgttatta ttgccacgcc
    12361 taccgattat gacccgaaaa caaattattt taatacctca agcgtggaag cggtcatccg
    12421 tgatgtgaca gaaattaatc ccaacgcggt aatggtgatt aaatctacaa ttccggtagg
    12481 tttcaccaaa tcaattaaag aacgtctggg tatcgataac cttattttct cccccgaatt
    12541 cctccgcgaa ggtaaagccc tttacgataa ccttcatccg tcacgtattg tcatcggtga
    12601 gcgttcagaa cgcgcagaac gttttgcggc gttattacag gaaggcgcga ttaagcaaaa
    12661 tatcccaacc ctgtttaccg actccactga agcagaagcg attaaacttt tcgctaatac
    12721 ctatctggcg atgcgcgtag catactttaa tgaactggat agctatgcag aaagtttagg
    12781 tctgaatact cgccagatta tcgaaggcgt ttgtctcgac ccacgcattg gcaaccatta
    12841 caacaatccg tcgtttggtt atggtggtta ttgtctgccg aaagatacca agcagttact
    12901 ggcgaactac cagtctgtgc cgaataacct gatctcggca attgtcgatg caaaccgcac
    12961 gcgtaaagat tttattgccg atgccatttt gtcacgcaag ccgcaagtgg tgggtattta
    13021 tcgtctgatt atgaagagcg ggtcagataa cttccgtgcg tcttccattc aggggattat
    13081 gaagcgtatc aaggcgaaag gcgttgaagt aatcatttac gaaccggtaa tgaaagaaga
    13141 ctcattcttc aactctcgcc tggaacgtga tctcgccacc ttcaaacaac aagccgacgt
    13201 cattatctct aaccgtatgg cagaagagct taaggatgtg gcagacaaag tctacacccg
    13261 cgatctcttt ggcagtgact aacatcctgt tatcagggcg attttcgccc tgattctctt
    13321 atgttccctt tgaaataatt cattattttt atcatttatc ctatagcatt catggcgatt
    13381 atcgctaaac tatggcgact taaaattatt tcgtccgtta gggtaatgat gagagtagaa
    13441 aataataatg tttctgggca aaaccatgac ccggaacaga ttgatttgat tgatttactg
    13501 gtgcagttgt ggcgtggcaa gatgacaatt atcatctctg tcattgttgc tattgccctg
    13561 gctatagggt atctggccgt tgccaaagaa aagtggactt ccacagctat cgttactcaa
    13621 cctgatgtgg gacaaatcgc tggttataac aatgccataa atgtgattta tggctcggca
    13681 gcgccgaaag tgtctgagat ccagtcgatg ttaattggtc gtttcagcac gacattttct
    13741 gcattagcag aaacgctgga taatcaggaa gaacctgaaa aactgaccat tgagcctacc
    13801 gttaaaaatc agtcattacc tttagcggtt tcttatgtgg gtgaaacacc tgaaggtgcg
    13861 cagaaacagc tggcgcagta tatccagcaa gttgacgatc aggtaaatga agaactggaa
    13921 caagacctga aagacaacat cgcgctgcaa atgaaaaact tgcaggattc tctgaagacc
    13981 caggaagtgg ttgcgcagga gcagaaagat ctgcgtatcc gtcagattca ggaagcgttg
    14041 cagtatgcga atcaggcgca ggtgacaaag ccgcagattc agcagacaca ggacgtaacc
    14101 caagatacta tgttcctttt ggggagcgaa gcgctggagt cgatgattaa gcatgaggcg
    14161 actcgtccgt tggtgttctc atcaaactac tatcagactc gtcaaaacct gctggatatc
    14221 gacaatcttg acgttgataa acttgatatt catgcttatc gctatgtgat gaaaccgacg
    14281 ttacctattc gtcgcgatag cccgaaaaag gcaatcacct tgattttggc ggtgctgctg
    14341 ggcggcatgg ttggcgcggg gattgtgctg gggcgtaacg ctctgcgtaa ttacaacgcg
    14401 aagtaatctt ttcggtttta aagaaaaagg gcagggtggt gacaccttgc ccgttttttt
    14461 tgccggatgc tacaacaata tcgcatccgc ttaccccgca actcactgat gccgtttacg
    14521 caggttctca atcaccgtcg ttaagtccag atcctgatcc tgcaacagca ccagcaggtg
    14581 atacatcaaa tcagatgcct cgttggtcag ctcaaagcgg tcatgtactg tcgctgccag
    14641 tgcggtttcc accccttctt cgcccacttt ctgcgcaatg cgtttggtgc cgctggcata
    14701 cagtttggcg gtataggagg ttttcgggtc ggcagattta cgttcggcga gcagttgttc
    14761 cagttgataa aggaacagcc actggtgaac ggtgtcgccg aagcagctgc tggtgccttt
    14821 gtggcaggtt ggaccaatgg gattcgccag caccagtaac gtgtcgttgt cgcagtccgg
    14881 cacaatactc accacgttga ggaaattgtc cgacgtttcg cctttggtcc acagtcgctg
    14941 tttagtgcgc gagaagaagg tgactttgcc gctttcgatg gttttgtcta aggcttccgg
    15001 gttcatgtag cccagcatta acacttcgcc ggatactgca tgttgcacaa tcaccggcat
    15061 cagtccgtcg gttttttccc agtccagttc gcgacgttgt tgttctgtta acatatcctg
    15121 atctccacgc cctgtgttgc caggtacgct tttaattcac caatattgat tatttgtttg
    15181 tggaatacgg aagctgccag cgcgccgtca acgtcggcat cgcggaaggc ttcgaggaag
    15241 tgttccatgg tccccgcgcc accggaggca atcagcggga cgtggcaaac ttcacgtact
    15301 tttttcagct gctcgaggtc gtaaccgtta cgcacgccgt cctgattcat catgttgagg
    15361 acgatttctc cggcaccgcg tttttgcact tcctgtaccc agtcgagcgt ttcccactga
    15421 gtgacgcggg tgcggctttc atc
//
