LOCUS       DQ000314               10485 bp    DNA     linear   BCT 29-JUN-2007
DEFINITION  Escherichia coli O77 O antigen gene cluster, complete sequence.
ACCESSION   DQ000314
VERSION     DQ000314.1  GI:62959549
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Wang,W., Perepelov,A.V., Feng,L., Shevelev,S.D., Wang,Q.,
            Senchenkova,S.N., Han,W., Li,Y., Shashkov,A.S., Knirel,Y.A.,
            Reeves,P.R. and Wang,L.
  TITLE     A group of Escherichia coli and Salmonella enterica O antigens
            sharing a common backbone structure
  JOURNAL   Microbiology (Reading, Engl.) 153 (PT 7), 2159-2167 (2007)
   PUBMED   17600060
REFERENCE   2  (bases 1 to 10485)
  AUTHORS   Feng,L., Perepelov,A.V., Wang,W., Shevelev,S.D., Wang,Q.,
            Senchenkova,S.N., Li,Y., Shashkov,A.S., Knirel,Y.A., Reeves,P.R.
            and Wang,L.
  TITLE     Structure Characterization of Escherichia coli O73 O Antigen and
            Identification of O Antigen Gene Clusters of Several E. coli
            Strains Which Share the Common O antigen Backbone with Different O
            Antigen Modification Factors
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 10485)
  AUTHORS   Feng,L., Perepelov,A.V., Wang,W., Shevelev,S.D., Wang,Q.,
            Senchenkova,S.N., Li,Y., Shashkov,A.S., Knirel,Y.A., Reeves,P.R.
            and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2005) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P.R. China
FEATURES             Location/Qualifiers
     source          1..10485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O77"
     misc_feature    1..10485
                     /note="O antigen gene cluster"
     gene            1159..2163
                     /gene="wbaC"
     CDS             1159..2163
                     /gene="wbaC"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbaC"
                     /protein_id="AAY23735.1"
                     /db_xref="GI:62959550"
                     /translation="MKVIVNNFFDGILKRGIPIYTSELVQKLRDNNVQVIYVACPKAF
                     HKLPSWFLNVLFIIVEQLLIPIMGIFYRTKYNIYPYNSSSIIDLLLGKAIVIIHDFIS
                     LKKNKRNLAAIYVKVCIYFSSFLAKKVIFISQSTQRVSKRISLFKCIESILLPNPFFS
                     FEKIANVTEIKDLGYLLLVSGLGDNKDLGTALDYYFSIPREERIPLKILGCGNGISKV
                     NDLIDGRDTDKEIEIIGFVSLEEVVKLYCNSKIVWAHSLAEGYGRTLAEAKLTCKNVI
                     CTRISAFREQKDSNIFFYTYYSEYYEVYSYLVDNSPSVERKALHEHILFENELRKIYE
                     "
     gene            2156..3358
                     /gene="wbaD"
     CDS             2156..3358
                     /gene="wbaD"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbaD"
                     /protein_id="AAY23736.1"
                     /db_xref="GI:62959551"
                     /translation="MSNIDINKKQILVLTPRFPFPVIGGDRLRIYKICKELSKHYDLT
                     LLSLCDKREELNYEYDREVFSSVHRVYLSKKKSILNVIFSLFSNTPLQIGYYKSKEFE
                     DKLKQLLPEHSATLSHLIRVGDYVKENKDINFLEMTDAISLNYKRVKEKASLLSLKTF
                     VYSFEQKRLERYERTINNKFSLTTLVSQVDSDYLYPDRPNNVLVCGNGVDAVSLPFSE
                     RKIAKDKKITLVFIGNLYSLQNMDGVRWFTKEVLPFLNKHGNFEFKVIGRITDKDKSW
                     LESQPGVVVTGEVDSITYAAADGHIGVCPIRLGAGIQNKVLEYMALGLPCISSTVGFE
                     GLGAEEGKEIYVANTKEEYLRVLNYFITNLDKYTETALVAKKFIGENFSWEAKLSPYI
                     QKIKESVK"
     gene            3360..4775
                     /gene="manC"
     CDS             3360..4775
                     /gene="manC"
                     /note="mannose-1-phosphate guanylytransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="AAY23737.1"
                     /db_xref="GI:62959552"
                     /translation="MIIPIIMAGGSGTRLWPLSRSLYPKQFLSLINENSLLQETLKRL
                     DGLNCLPPVIVSNNEHRFIVAEQLRQFGVDDFQIILEPVGRNTAPAVALAALKSLELH
                     GDHHMLVLAADHAIQDIEAFHAAVLAAEQESVDNKLVTFGIVPTKPETGYGYIKKGEQ
                     VKNSVFKVNSFVEKPDLETAKNYLEQKCYLWNSGMFMFKASVYLDELKKFRPDILAAC
                     KESLSSASTDLDFIRLNSDVFAECPDESIDYAVMEKTQDCVVIPLDADWSDIGSWTSL
                     WEISEKDEHENVSHGDVINYNSRNNYIYSEGSLISTVGVNNLIIVQTKDALLVAQQDN
                     VQDIKKIVEILKKQKRSEHISHREVYRPWGRYDSVERGDRYQVKRITVKPGECLSTQM
                     HHHRAEHWVVVAGTAKVTCGERTFFVTENESTFIPIGTVHTLENPGKIPLEVIEIQSG
                     VYLGDDDIVRLSDKYGRVEDK"
     gene            4780..6201
                     /gene="manB"
     CDS             4780..6201
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="AAY23738.1"
                     /db_xref="GI:62959553"
                     /translation="MNITNDVIKNSGIAFGTSGARGLVKDFTPNVCAAFTYAFIDVMM
                     KDFSFKEVALAIXNRPSSYAMAQACAAALLECSIKPIYYGVIPTPALANQAIADGIPA
                     IMVTGSHIPFDRNGLKFYRPDGEISKENENSIIHAEKEYPDVTVLPELQCSKRAADTY
                     IERNTSIFSNIFKGKRIGIYEHSSAGRDLYSEIFSKLGAEVVSIGRSDEFVPIDTEAV
                     SKEDEEKALHWSSEYDLDMIFSTDGDGDRPLVADENGVWLRGDILGLFCSKAMNIEAV
                     AVPVSCNTVIQTCGWFNHVTLTKIGSPYVISAFDSLNNSFDRVAGFEANGGYLLGSDV
                     NYNSGMIKALPTRDAVLPALMVLALAIKNNVTISKLLAELPPRFTYSNRVQNFATSKS
                     KSIIASAQENPQEFIERIGFVDLHVQSVDTIDGLRLTLSDGSIIHLRPSGNAPELRCY
                     AEADNIIKAEKLVLEVLHKVTLL"
     gene            6385..7716
                     /gene="wzy"
     CDS             6385..7716
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="AAY23739.1"
                     /db_xref="GI:62959554"
                     /translation="MHKQYYSLLCNKIQFKHLAIYFSVFLSLSSCFLWGGADTFVSQV
                     YIDILATISTALLVVLMFYYYINRVRYLSLTLLFAFVFSLIIGLPSIYLYYFKNANHG
                     FEIICIWGMLINSILYLQTTETVIKEEKRYLTSLFYILFSIVALCQLYKILIYLMFIL
                     NSGEGHLAIYTESEELLSQVPFFIRAISGFAVVMSLSTFYFKTPTYIKVIAFVLLASD
                     LAIGIRNKFFFSVICIFILYMYSNEYRVRNLFKQLSKPIFLIAGFLCFSMVSYFREGY
                     QIDFMIYIGIVLDTLSSTLGGMQNLFDMQSSGWDQLHPAVVFTQVLPLSGLGFLDDQQ
                     IAKDFSIIVLGDVSSGIALSSSGLLESSLLSLNLGVIIYLLYLSGVMLFIKWGLNNQR
                     IIINFVAISMISGFFYAVRGELILPLAFLLKSLPIVLISPLLVKNKIYHGQ"
     gene            7758..8993
                     /gene="wzx"
     CDS             7758..8993
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="AAY23740.1"
                     /db_xref="GI:62959555"
                     /translation="MIKEIFSDGIFSLFNKICYFGFKTLAIILITRSLGEDVGGEFIF
                     LVGFLEVLRVVTDFGVDLFVIKKYNDSTDKIKLLNVTFAQKILSGVIVLLLFCLYSFV
                     QGYQYNVYIPIVLALPVALFFNFSNSYFQSLNSNKSLTFPIAIASIATCTIFAIQYFL
                     NVKFNAWDYLCVELLFVSLVTLKLFVSVKFRFIRTSAFDVLMSILSLYKYTLSIGLTA
                     VVVIIYSRIDNFYLKTFAPGDLAAYGQIFRMVDPLVMVSSVFSTVAYARFCRINLQEK
                     NAFTKVFQFLLCIVAYGVLSSLVYFFIIKYLGHFVLISNVNNDFLIMMFLIVACVKCI
                     NGGTTAIIQSQGFYKVGLYVSYVCILTSIPTMYFLIMKYGVKGAAMTILLVECISFIL
                     LLCSFFMIKKMRPKKTFEM"
BASE COUNT     3059 a   1813 c   2194 g   3418 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 caactactgg cggaagtgca gtccatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attttatgtg cacgacctgc cattggtgac
      241 aacccatttg tcgtggtact gccagacgtt gttatcgatg acgccagcgc cgatccgcta
      301 cgctataacc ttgctgccat gattgcgcgc ttcaacgaaa cgggccgcag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca ttcagaccaa agaaccactg
      421 gatcgtgaag gtaaagtcag ccgcatcgtc gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcacgcaacc tggtgcatgg gggcgtattc agctgactga tgctattgcc
      601 gaactggcga aaaaacagtc cgttgatgcc atgctgatga ccggcgacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttt gtgaagtatg gactgcgcaa cctgaaggaa
      721 gggccaaagt tccgtaagag cattgagaag ttgctcgggg aatagatccc aagccaatgt
      781 aacaataatt tagataaata gcggcagcta acatcccggc gtctatgctt taaacaaagg
      841 aatgttactg tcgtttttct ttttaaaaca aagaataaca acgagttaac ctactaagtt
      901 taatctattt tttgttggat ttatccttgt ttctgacagc atttggtaag acaattggcg
      961 tttgaattta agatagcttt gcgatagcta gcggggcggg gtacatcttt ggcattttgc
     1021 tagtgcactg gtagctgtta agccaggggc ggtagcgtgg taagattgtg tttatatgat
     1081 gcgttctcaa aataagaaat aataatcctg actattttta tactattgat aatatctgaa
     1141 ataaacttaa gagcaaacat gaaagtaatt gtaaataatt tttttgatgg tattttgaaa
     1201 agaggaatac cgatctatac ttctgaactt gtgcaaaagt taagagacaa taatgttcag
     1261 gttatttatg ttgcatgtcc aaaagcattc cataagctgc cttcatggtt tctgaatgtt
     1321 ctttttataa tagtagagca gcttttaata ccaattatgg gtatatttta tagaactaag
     1381 tataatatat atccctataa tagtagttcc atcattgact tgttattagg taaggcaatt
     1441 gttataattc atgacttcat tagtcttaag aaaaataaac gaaatcttgc tgccatttat
     1501 gtgaaagtct gtatttattt ttcatctttt ttagcaaaga aagtaatatt tatttctcaa
     1561 agcactcagc gagtatctaa gagaatatcg ctatttaagt gtatagaatc tatcctgctc
     1621 ccaaatccat ttttctcatt tgagaaaatt gctaatgtta ccgaaataaa agatcttgga
     1681 tatttacttc tagtttcagg tttaggggat aataaggatc taggcacagc tctggactat
     1741 tatttttcaa tcccaaggga agaaagaata ccattaaaaa tattaggttg tgggaatggt
     1801 attagcaagg ttaacgactt aattgatggg cgggatacag ataaagaaat tgaaattatt
     1861 ggttttgttt ctttagagga agtcgtcaaa ctatattgta attctaaaat tgtttgggct
     1921 cattcgcttg ccgaggggta tggaaggact ttagctgaag caaaactcac atgcaaaaat
     1981 gttatatgca cacggatctc tgctttccgc gaacaaaagg attcgaatat tttcttttat
     2041 acttactatt ctgagtatta tgaagtctat tcatacttgg tagataattc acccagtgtc
     2101 gaaaggaaag ctctgcatga acatatttta tttgaaaatg aattgagaaa gatttatgag
     2161 taatattgat ataaataaga aacagatatt agttttgact ccacggttcc cgtttcctgt
     2221 cattggtggg gatcgattac gtatctataa gatctgtaaa gaactgtcta agcattatga
     2281 tcttacgttg ctgagtctgt gtgataaacg cgaagagctc aactatgaat atgatcgtga
     2341 agtttttagt agtgttcaca gagtatatct ttcaaagaaa aaatctattt taaatgtcat
     2401 attctctttg ttttcaaata ctcccctaca gattgggtat tataaatcta aagaatttga
     2461 agataaactt aagcagttat tacctgaaca ttctgcaact ctttcacatt tgataagggt
     2521 tggtgattat gtgaaagaga ataaagatat aaatttttta gagatgaccg atgccatttc
     2581 acttaattat aaacgagtaa aggaaaaagc atctttattg agtttaaaaa catttgtata
     2641 ttcttttgaa caaaaaagac tcgaacgata tgagcgaact ataaataaca agttcagctt
     2701 gactactttg gtatcacagg ttgactccga ttatttatat ccagaccgtc cgaataatgt
     2761 attagtctgt ggaaatggtg ttgatgccgt tagcttacct ttttcagaac gaaaaatagc
     2821 taaagataaa aagataaccc ttgtatttat aggtaattta tattctttgc aaaatatgga
     2881 tggtgtgaga tggttcacca aggaagtttt accatttctt aacaagcatg gtaattttga
     2941 atttaaggtc atcggacgta ttacagataa agataagagt tggttagaaa gccagcctgg
     3001 cgttgttgtc acaggtgaag ttgatagcat aacttatgca gcagctgatg ggcatattgg
     3061 tgtatgtcct attagattag gggcaggtat ccaaaataag gtacttgaat atatggcgtt
     3121 gggattacct tgtatctctt ctactgtagg ctttgaaggc ttgggagcag aggagggtaa
     3181 ggagatttat gttgccaata caaaagaaga atatttacgt gtattaaatt attttataac
     3241 aaatctggat aaatataccg aaacagcatt agttgcgaaa aagtttatcg gtgagaattt
     3301 ttcatgggaa gctaaattga gcccatatat ccagaaaatt aaggagtctg ttaaataaaa
     3361 tgattatccc aattattatg gccggtggtt ctggtactcg cttatggcct ctttcgcgtt
     3421 cgttataccc gaaacaattt ttatcattga ttaatgaaaa tagtttgctc caggaaacgt
     3481 tgaagagact tgatggatta aattgtttgc ctcctgtcat tgttagcaat aatgagcata
     3541 gatttattgt tgctgaacaa ttgcgacagt tcggtgtgga tgacttccag attattctag
     3601 aacctgtagg tagaaatact gcgcccgccg ttgcgctggc tgctctaaag tctctagaac
     3661 tccatggcga tcatcatatg ctggttcttg ctgctgacca tgctattcag gatattgaag
     3721 ccttccatgc agctgtttta gctgcagaac aagagtccgt tgacaataag ttagtgactt
     3781 ttggcatcgt cccgaccaaa cctgaaactg ggtatggcta tattaaaaag ggcgaacaag
     3841 ttaaaaactc tgtatttaaa gtaaattctt tcgtcgaaaa acctgattta gaaacggcta
     3901 aaaattatct cgaacaaaaa tgctatttgt ggaatagtgg tatgttcatg ttcaaggcga
     3961 gcgtatattt agatgaattg aaaaaatttc gccctgatat tttagcagcg tgtaaagagt
     4021 cgctttccag tgctagtact gatctagatt ttatcagatt aaattctgat gtctttgcag
     4081 aatgtcccga tgagtcaatt gattatgcgg taatggaaaa aactcaggat tgtgtagtta
     4141 ttccacttga tgctgactgg agtgacatcg gttcatggac atcactttgg gaaatatcag
     4201 agaaagatga acatgaaaac gtatcgcatg gcgatgtaat taactataac agcaggaaca
     4261 actacattta ctctgaaggt tcactgatca gcactgtagg agttaataac cttattatag
     4321 ttcaaactaa agatgcgctc ctggtagcac agcaagacaa cgtacaagac ataaagaaga
     4381 ttgtcgaaat tcttaaaaag caaaaacgta gcgaacatat atcccatcgc gaagtctatc
     4441 gtccttgggg acgttatgat tctgtagaac gtggcgaccg ttatcaggta aagcgtatta
     4501 ccgttaagcc tggtgaatgt ctctcaacac aaatgcatca tcatcgagct gagcattggg
     4561 tggttgtcgc tggtactgca aaggtgactt gcggcgaacg cacttttttc gtcactgaaa
     4621 atgagtcaac tttcatacca attggcacag ttcatacctt ggaaaatccg ggaaagatac
     4681 cattagaagt aattgagatt cagtcgggtg tatatctagg tgatgatgat attgtgcgcc
     4741 taagtgataa atatggtcgt gttgaggata aataattata tgaatattac caatgatgta
     4801 attaaaaata gcggaattgc atttggtaca agtggtgctc gtggattagt gaaagatttt
     4861 acacccaacg tgtgtgctgc attcacttat gcttttatcg atgtaatgat gaaggatttc
     4921 tcttttaaag aggttgcgct cgcaatanat aatcgcccaa gcagctatgc tatggcacaa
     4981 gcttgcgcag cagcattgtt ggaatgtagt attaaaccta tttactacgg tgtaattcca
     5041 actccagctt tggcaaatca agcgattgcc gatgggattc ctgcaataat ggttactgga
     5101 agtcatattc catttgatag aaatggtttg aaattctatc gtccagacgg ggaaatttca
     5161 aaagaaaatg agaacagcat tatacatgct gaaaaagagt atcctgatgt aactgtattg
     5221 ccagaattac aatgttcaaa gcgtgccgct gatacttata tcgagagaaa cacttcaatt
     5281 ttttcaaata tatttaaagg taaacgtatc gggatttatg aacattcaag tgcgggacgg
     5341 gatctttatt cagaaatatt cagtaagctt ggtgctgaag ttgtaagtat tggtagaagt
     5401 gatgagtttg tgcctattga tactgaagca gtatcaaaag aagatgaaga aaaagcttta
     5461 cactggtcat ctgagtatga cctcgatatg attttttcta ccgatggtga tggcgatcgt
     5521 cctttggttg ctgacgaaaa tggcgtttgg ttacggggtg atatattagg actattttgc
     5581 tcaaaggcaa tgaatattga agcggtagct gtacctgtaa gttgtaatac ggttattcag
     5641 acttgcgggt ggtttaatca tgtaacgctg actaaaattg gttcaccata cgtcatttca
     5701 gcgtttgatt ctctgaataa tagttttgat agggttgcag ggtttgaggc taacggtggg
     5761 tatcttttag gtagcgatgt aaattataac agcggtatga ttaaagcgct tccaacaaga
     5821 gatgctgttt tgcctgcttt aatggtactg gcactggcta taaaaaataa tgtgactatt
     5881 tcaaaactct tggcggagtt gccaccacga tttacctact caaatagagt tcaaaatttt
     5941 gcaacaagca aaagcaaaag cattatcgcg tcagctcaag aaaatcctca agaatttatc
     6001 gaaagaatag gctttgtcga tcttcatgta caatcggtgg atacaattga cgggttaaga
     6061 ttgacactaa gtgatggttc aataatccat ttgcgaccgt caggcaacgc acctgaattg
     6121 cgatgctatg cagaagcaga taacataata aaagctgaaa aattagtgtt agaagttttg
     6181 cataaagtta ctcttttgta aaaaaatata aacttttttc tactcttgag cggtttgtca
     6241 tttaaccgct cagtaataga ttgtttataa attgtttata tttagcattg gtgtttttat
     6301 ttgcgttcat cccgaataag agattgtaaa attaactgtt tcttattttt caaatgagtt
     6361 acttcactag taaagctaat tactatgcat aagcaatatt attcattatt gtgcaataaa
     6421 atacagttta aacatctggc tatatatttt tctgtatttc tttcactatc ctcatgtttc
     6481 ttgtggggag gggcggatac atttgtatcg caagtgtata ttgatatttt agccactata
     6541 tctacagcgc tattagttgt tttgatgttc tattattata ttaatagagt tcgctattta
     6601 tcactaacct tactttttgc attcgttttt tctttgatta ttggtttgcc atcgatttat
     6661 ctttactatt ttaaaaacgc aaatcatggg tttgaaataa tttgtatctg ggggatgttg
     6721 atcaattcca tcctttactt gcaaacaaca gagactgtca taaaagaaga aaaaagatat
     6781 ttaactagtt tattttatat tttgttttct attgttgcac tgtgtcaatt atacaaaata
     6841 ttaatttatc ttatgtttat attgaattca ggagaagggc atttagcaat ttatactgaa
     6901 agcgaagaat tgctatccca agttccattt tttattcgtg ctattagtgg ttttgcagtt
     6961 gtaatgtcat tgtccacttt ttattttaaa acaccgacct acattaaagt tattgcattt
     7021 gttttacttg catctgattt agctattgga atacgtaata agtttttctt ttcagtgatt
     7081 tgtattttta tactatatat gtactctaat gagtatagag taagaaattt atttaagcaa
     7141 ctctcaaaac caatatttct catcgccggt tttttatgct tctcgatggt atcgtatttc
     7201 cgtgaagggt accaaatcga ttttatgatt tatattggca ttgttttaga tacgctatcc
     7261 tctactcttg gggggatgca gaatttgttt gatatgcaat catcaggatg ggatcaactt
     7321 catcccgctg tggtattcac acaagtatta ccgctaagcg gtcttggttt tcttgatgat
     7381 caacaaatcg caaaagattt ttcaataatt gttttaggtg atgtatcctc tggtattgcg
     7441 ttgtcatctt caggattgtt agagtcatcg ttgctgagtc taaacttggg agtgattatt
     7501 tatttactgt atttatcagg ggtgatgctt tttattaaat ggggactaaa taatcaacgg
     7561 ataataataa actttgttgc catctccatg atatctggat ttttttatgc tgttcgtggt
     7621 gaactgattt tgccattggc ttttcttcta aaatcattgc cgattgttct tatatctcca
     7681 ttacttgtta aaaataaaat ttatcatggt cagtaagtgg tttatttttc gaccaggatc
     7741 tcaccagtag gcatgacatg atcaaagaaa ttttctccga tggcattttt tcgctattta
     7801 ataaaatatg ttacttcggt tttaaaacac ttgcaataat tctgattact cgttccttag
     7861 gagaagatgt aggcggagag ttcatttttt tagttggttt tctcgaagtc cttcgggtgg
     7921 ttactgattt tggtgtggat ctgtttgtta taaaaaaata taatgattct acagataaaa
     7981 taaaactatt aaatgtaacg tttgcacaaa aaattctttc tggagtaatt gttttactac
     8041 tgttttgttt gtattctttt gttcagggtt atcaatataa tgtctatatt cctattgtgt
     8101 tagccttacc tgttgcttta ttttttaatt tctcaaatag ctattttcaa tctctcaatt
     8161 ccaataaatc tctcacgttt ccgatcgcaa ttgcatctat tgcaacgtgt acgatcttcg
     8221 ctattcagta ttttttaaac gttaaattta acgcatggga ttacctgtgt gttgaactat
     8281 tattcgtatc gctggttaca ctaaaactat tcgttagtgt caagtttagg tttattagaa
     8341 cgtcagcttt tgatgtgcta atgtcaattc tttctttata taaatataca ttaagtatag
     8401 gtcttacagc tgttgttgta attatttata gccgtataga taatttttat ctcaagacat
     8461 ttgccccagg ggatcttgca gcatatggcc agatatttag gatggttgat ccactggtta
     8521 tggtttcttc tgtattttct acagttgcct atgcaagatt ttgccgaatt aacttacaag
     8581 aaaaaaatgc ttttactaag gtgtttcagt tcctgttatg tattgtggca tatggcgtgc
     8641 tgagttcatt agtttatttc tttataataa aatacctggg tcattttgta ttgatttcta
     8701 atgttaataa tgatttcttg ataatgatgt tcttgattgt tgcatgtgtg aaatgtatca
     8761 atggtggtac aactgcaata attcagtcac aggggtttta taaagtgggt ttgtatgttt
     8821 cgtatgtttg cattttgaca tcgataccaa ctatgtattt cttaataatg aagtacggtg
     8881 ttaaaggtgc tgctatgacg attctgcttg tagagtgtat tagtttcata ttacttctct
     8941 gcagcttttt tatgataaaa aagatgcgtc ctaaaaaaac attcgaaatg taatcaatga
     9001 gtcctctttg aatagaatta gtttgttcta tttatgaaca cttagcttga gtaagttcta
     9061 acatagatta ctaattgttt gatactatct gctttacaag cataaccgcg tatgtcgcgg
     9121 tgaccacacc tgacaggagt atgtaatgtc caagcaacag atcggcgtcg tcggtatggc
     9181 agtgatgggg cgcaacctgg cgctcaacat cgaaagccgt ggttataccg tctccgtctt
     9241 caaccgctcc cgcgaaaaaa ctgaagaagt tgttgccgag aacccgggta agaaactggt
     9301 tccttattac acggtgaaag agtttgtcga gtctcttgaa acccctcgtc gcatcctgtt
     9361 aatggtgaaa gcaggtgcag gcacggatgc tgctattgat tccctcaaac catatctcga
     9421 taaaggtgac atcatcattg atggtggtaa caccttcttc caggacacta ttcgtcgtaa
     9481 tcgtgagctt tcagcagagg gctttaactt catcggtacc ggtgtttccg gcggtgaaga
     9541 gggggcgttg aaaggacctt ccatcatgcc tggtgggcag aaagaagcct atgaactggt
     9601 tgcaccgatc ctgaccaaaa tcgccgcagt agctgaagac ggtgagccat gcgttaccta
     9661 tattggtgcc gatggcgcag gtcactatgt gaagatggtt cacaacggta ttgaatacgg
     9721 cgatatgcag ctgattgctg aagcctattc tctgctaaaa ggtggcctga acctcaccaa
     9781 cgaagaactg gcgcagactt ttaccgagtg gaataacggt gaactgagca gctacctgat
     9841 cgacatcacc aaagatatct tcaccaaaaa agatgaagac ggtaactacc tggttgatgt
     9901 gattctggat gaagcagcaa acaaaggtac gggcaaatgg accagccaga gtgcgctgga
     9961 tctcggcgaa ccgctgtcgc tgataaccga gtctgtgttt gcacgttata tctcttctct
    10021 gaaagatcag cgcgttgccg cgtctaaact tctctctggc ccgcaagcgc agccagcagg
    10081 cgacaaggct gagttcatcg aaaaagttcg ccgtgcgctg tatctgggca aaatcgtttc
    10141 ttacgctcag ggcttctctc agctacgcgc cgcgtctgaa gagtacaact gggatctgaa
    10201 ctacggcgaa atcgcgaaga ttttccgtgc tggctgcatc atccgtgcgc agttcctgca
    10261 gaaaatcacc gatgcttatg ccgaagatcc gcagatcgct aacctgctgc tggctccgta
    10321 cttcaagcaa attgctgatg actaccagca ggcgctgcgt gatgtcgttg cttatgcagt
    10381 acaaaacggt atcccggttc cgaccttcgc cgctgcggtt gcctattacg atagctaccg
    10441 tgccgctgtt ctgcctgcga acttaattca ggcccagcgc gacta
//
