LOCUS       AB010150               15295 bp    DNA     linear   BCT 21-MAY-1998
DEFINITION  Escherichia coli O8 wb gene cluster, complete cds.
ACCESSION   AB010150
VERSION     AB010150.1  GI:3142207
KEYWORDS    mannosyltransferase; ATP binding component of ABC-transporter;
            phosphomannomutase; GDP-mannose pyrophosphorylase;
            gluconate-6-phosphate dehydrogenase; integral membrane component of
            ABC-transporter.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Sugiyama,T., Kido,N., Kato,Y., Koide,N., Yoshida,T. and Yokochi,T.
  TITLE     Generation of Escherichia coli O9a serotype, a subtype of E. coli
            O9, by transfer of the wb* gene cluster of Klebsiella O3 into E.
            coli via recombination
  JOURNAL   J. Bacteriol. 180 (10), 2775-2778 (1998)
   PUBMED   9573168
REFERENCE   2
  AUTHORS   Sugiyama,T., Kido,N., Koide,N., Kato,Y., Yoshida,T., Jiang,G.,
            Paeng,N., Takahashi,K., Morikawa,A. and Yokochi,T.
  TITLE     Nucleotide sequence of Escherichia coli O8 wb gene cluster
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 15295)
  AUTHORS   Sugiyama,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-1998) Tsuyoshi Sugiyama, Aichi Medical
            University, Department of Microbiology and Immunology; Yazako,
            Nagakute, Aichi 480-11, Japan (E-mail:sugiyama@aichi-med-u.ac.jp,
            Tel:0561-62-3311, Fax:0561-63-9187)
FEATURES             Location/Qualifiers
     source          1..15295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F492"
                     /serotype="O8"
                     /db_xref="taxon:562"
                     /clone="pTSO8"
                     /clone_lib="31"
     -35_signal      44..49
                     /note="putative"
     -10_signal      70..75
                     /note="putative"
     RBS             135..139
     gene            146..1552
                     /gene="gnd"
     CDS             146..1552
                     /gene="gnd"
                     /EC_number="1.1.1.44"
                     /note="putative"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate-6-phosphate dehydrogenase"
                     /protein_id="BAA28321.1"
                     /db_xref="GI:3142208"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIA
                     ENPGKKLVPYYTVQEFVESLETPRRILLMVKAGAGTDSAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILKQIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYALLKGGLALSNEELAQT
                     FTEWNEGELSSYLIDITKDIFTKKDEEGKYLVDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYNWELNYGEIAKIFRAGCIIRAQFLQKITDAYAQKAGIANLLLAPY
                     FKQIADDYQQALRDVVAYAYQNGIPVPTFSAAIAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWLE"
     terminator      1565..1590
                     /note="putative"
     -35_signal      1696..1701
                     /note="putative"
     -10_signal      1722..1727
                     /note="putative"
     RBS             1784..1787
     gene            1795..3210
                     /gene="manC"
     CDS             1795..3210
                     /gene="manC"
                     /EC_number="2.7.7.22"
                     /note="putative"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphorylase"
                     /protein_id="BAA28322.1"
                     /db_xref="GI:3142209"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLYGQNSMLQETITRL
                     SGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRYG
                     DDPLMLVLAADHIINNQSAFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGMA
                     LTDSAHTPYQVARFVEKPDRDRAEAYLASGEYYWNSGMFMFRAKKYLSELAKFRPDIL
                     EACQAAVNAADNGSDFISIPHDIFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSW
                     SALWEVSPKDEQGNVLSGDAWVHNSENCYINSDEKLVAAIGVENLVIVSTKDAVLVMN
                     RERSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFS
                     MQMHHHRVEHWVILAGTGQVTVNGKQFLLSENQSTFIPIGAEHCLENPGCIPLEVLEI
                     QSGSYLGEDDIIRIKDQYGRC"
     RBS             3225..3227
     gene            3234..4610
                     /gene="manB"
     CDS             3234..4610
                     /gene="manB"
                     /EC_number="5.4.2.8"
                     /note="putative"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="BAA28323.1"
                     /db_xref="GI:3142210"
                     /translation="MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVG
                     GDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     NYNGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLRQISVLKEYVDHLM
                     GYVDLANFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGI
                     PNPLLPECRQDTADAVREHQADMGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFL
                     QKQPGAKIIHDPRLTWNTVDIVTRNGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHH
                     YFRDFAYCDSGMIPWLLVAELLCLKNSSLKSLVADRQAAFPASGEINRKLGNAAEAIA
                     RIRAQYEPAAAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADVALMNEKTTE
                     LLHLLSGE"
     RBS             4604..4606
                     /gene="manB"
     gene            4612..5406
                     /gene="wzm"
     CDS             4612..5406
                     /gene="wzm"
                     /note="putative"
                     /codon_start=1
                     /transl_table=11
                     /product="integral membrane component of ABC-transporter"
                     /protein_id="BAA28324.1"
                     /db_xref="GI:3142211"
                     /translation="MRDLLTTIYRYRGFIWSSVKRDFQARYQTSMLGALWLVLQPLSM
                     ILVYTLVFSEVMKARMPDNTGSFAYSIYLCSGVLTWGLFTEMLDKGQSVFINNANLIK
                     KLSFPKICLPIIVTLSAVLNFAIIFSLFLIFIIVTGNFPGWLFLSVIPVLLLQILFAG
                     GLGMILGVMNVFFRDVGQLVGVALQFWFWFTPIVYVLNSLPAWAKNLMMYNPMTRIMQ
                     SYQSIFAYHLAPNWYSLWPVLALAIIFCVIGFRMFRKHAADMVDEL"
     RBS             5394..5396
                     /gene="wzm"
     gene            5406..6620
                     /gene="wzt"
     CDS             5406..6620
                     /gene="wzt"
                     /note="putative"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP binding component of ABC-transporter"
                     /protein_id="BAA28325.1"
                     /db_xref="GI:3142212"
                     /translation="MSYIRVNNVGKAYRQYHSKTGRLIEWLSPLNTKRHNLKWILSDI
                     NFEVAPGEAVGIIGINGAGKSTLLKLITGTSRPTTGEIEISGRVAALLELGMGFHSDF
                     TGRQNVYMSGQLLGLSSEKITELMPQIEEFAEIGDYIDQPVRVYSSGMQVRLAFSVAT
                     AIRPDVLIIDEALSVGDAYFQHKSFERIRKFRQEGTTLLLVSHDKQAIQSICDRAILL
                     NKGQIEMEGEPEAVMDYYNALLADKQNQSIKQVEHNGKTQTVSGTGEVTISEVHLLDE
                     QGNVTEFVSVGHRVSLQVNVEVKDDIPELVVGYMIKDRLGQPIFGTNTYHLNQTLTSL
                     KKGEKRSFLFSFDARLGVGSYSVAVALHTSSTHLGKNYEWRDLAVVFNVVNTEQQEFV
                     GVSWLPPELEIS"
     RBS             6608..6611
                     /gene="wzt"
     gene            6620..7897
                     /gene="ORF425"
     CDS             6620..7897
                     /gene="wbbD"
                     /note="unknown"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAA28326.1"
                     /db_xref="GI:3142213"
                     /translation="MGSSFYRSFEERHRGSVEEIKRRLSFYLPFLAGLKDIYPDGVIA
                     DIGCGRGEWLEILNENGIVNIGVDLDDGMLARAREAGLNVQKMDCLQFLQSQADQSLI
                     ALTGFHIAEHLPFEVLQQLVMHTLRVLKPGGLLILETPNPENVSVGTCSFYMDPTHNH
                     PLPPPLLEFLPIHYGFNRAITVRLQEKEVLQSPDAAVNLVDVLKGVSPDYSIIAQKAA
                     PTDILERFDTLFTQQYGLTLDALSNRYDAILRQQFSSVVSRLETLNQTYMQQISQMSE
                     TIQTLQGEVDDLSHVIDQNHQLHQQMADLHNSRSWRITQPLRWLSLQRQLLRQEGAKV
                     RARRAAKKILRKGMALSLVFFHRYPKSKVYLFKVLRKTGCYTLLQRLFQRVMLVQSDT
                     MMMQSRRYDVGTEEMTSRAMSIYNELKNKNTEK"
     RBS             7888..7891
                     /gene="ORF425"
     gene            7900..9309
                     /gene="ORF469"
     CDS             7900..9309
                     /gene="wbdA"
                     /note="putative"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAA28327.1"
                     /db_xref="GI:3142214"
                     /translation="MRIVIDLQGAQTESRFRGIGRYSIAIARGIIRNNSRHEIFIALS
                     AMLDESIADIKAQFADLLPAENIVVWHAVGPVRAMDQGNEWRRESAELIREAFLESLC
                     PDVVFITSLFEGHVDDAATSVHKFSRQYKVAVLHHDLIPLVQAETYLQDDVYKPYYLQ
                     KVEWLKNADLLLTNSAYTAQEAIEHLHLQGDHVQNIAAAVDSQFCMAEVTASEKETVL
                     GHYGIQREFMLYAPGGFDSRKNFKRLIEAYAGLSDALRRSHQLVIVSKLSIGDRQYLE
                     SLASGNGLQQGELVLTGYVPEDELIQLYRLCKLFIFASLHEGFGLPVLEAMSCGAPVI
                     GSNVTSIPEVIGNPEALFDPYSVSSMRDKIAQCLTDDTFLARLKDMAQQQARNFSWDK
                     AAVTALEAFEKIAVEDTGTAQVLPEALIQKILAISQGQPDDRDLRLCATAIDYNLKTA
                     ELYQIDDKSLNWRVEGPFG"
     gene            9428..11542
                     /gene="ORF704"
     CDS             9428..11542
                     /gene="wbdB/C"
                     /note="putative"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAA28328.1"
                     /db_xref="GI:3142215"
                     /translation="MAQSENSDLLAFYNQCQTRKSNEKIDILSRNIYPPRVTKMDAKV
                     KFLHCYAWEETGFPQPWINEFNRELDGVLCTSEHVRKILIDNGLNVPAFVVGNGCDHW
                     LNIPAETTKDVDHGTFRFLHVSSCFPRKGIQAMLQAWGRAFTRRDNVILIIKTFNNPH
                     NEIDAWLAQAQAQFIDYPKVEVIKEDMSATELKGLYESCDVLVAPGCAEGFGLPIAEA
                     MLSGLPAIVTNWSGQLDFVNSQNSWLVDYQFTRVKTHFGLFSSAWASVDIDNLIDALK
                     AAASTDKSVLRDMADAGRELLLQQFTWKAVADRSCRAVKTLRGHIDIAQHRARIGWLT
                     TWNTKCGIATYSQHLVESAPHGADVVFAPQVSAGDLVCADEEFVLRNWIVGKESNYLE
                     NLQPHIDALRLDVIVIQFNYGFFNHRELSAFIRRQHDAGRSVVMTMHSTVDPLEKEPS
                     WNFRLAEMKEALALCDRLLVHSIADMNRLKDLGLTANVALFPHGVINYSAASVTRQQQ
                     SLPLIASYGFCLPHKGLMELVESVHRLKQAGKPVRLRLVNAEYPVGESRDLVAELKAA
                     AQRLGVTDLIDMHNDFLPDAESLRLLSEADLLIFAYQNTGESASGAVRYGMATQKPVA
                     VTPLAIFDDLDDAVFKFDGCSVDDISQGIDRILNSIREQDAWATRTQQRADAWREQHD
                     YQAVSRRLVNMCQGLAKAKYFK"
     -35_signal      11534..11539
                     /gene="ORF704"
                     /note="putative"
     -10_signal      11557..11562
                     /note="putative"
     RBS             11579..11582
     terminator      13886..13906
                     /note="putative bi-functional"
BASE COUNT     3607 a   3701 c   4063 g   3924 t
ORIGIN
        1 aagctttgtg catttatagt ccaaaattta tatcttgtgc tagttaacaa attttcacta
       61 tgagctcttt atactttggg ctaacattat tatcacattt aagctgcgaa cgtgtcgcag
      121 tgaccacacc agacaggagt atgtaatgtc caagcaacag atcggggttg tcggtatggc
      181 tgtgatgggg cgtaaccttg cgctgaatat cgaaagccgt ggttataccg tctccgtttt
      241 caaccgctcc cgtgaaaaga ccgaagaagt gattgccgaa aacccaggca agaaactggt
      301 tccttactac acagtacaag agtttgttga atccctcgaa acaccacgtc gtatcctgtt
      361 gatggtgaag gccggggctg gcaccgacag cgccatcgat tccctgaagc cttacctcga
      421 caaaggcgac atcatcattg atggcggcaa tactttcttc caggatacca tccgtcgtaa
      481 ccgtgaactg tctgccgaag gttttaactt tatcggtacc ggtgtttccg gtggagaaga
      541 gggggctctg aaagggcctt ccatcatgcc cggtggccag aaagaagcct atgagctggt
      601 tgctccaatt ctgaagcaga ttgctgcggt tgctgaagac ggcgagccat gtgtcaccta
      661 tatcggtgct gatggtgctg gtcattatgt aaagatggtt cataacggca tcgagtatgg
      721 cgatatgcaa ctcattgcag aagcttatgc gctgttgaaa ggcggtctgg ctctttccaa
      781 cgaagagctg gcgcaaacct tcaccgaatg gaacgaaggt gaactgagca gttacctgat
      841 cgacattacc aaagatatct tcaccaagaa ggatgaagaa ggtaaatacc tcgttgatgt
      901 cattcttgat gaagctgcaa acaaaggtac cggtaagtgg accagccaga gctcactgga
      961 tctcggcgaa cctctgtctc tgatcaccga gtccgtattt gctcgttaca tttcttcgct
     1021 taaagaccag cgtgtagctg cttcgaaagt gctgagtggt ccgcaggctc aaccagccgg
     1081 cgataaagca gaatttatcg aaaaagtgcg tcgcgcgttg tacctcggta aaatcgtctc
     1141 ctatgctcag ggcttctccc agctacgagc tgcttctgat gaatacaact gggagctgaa
     1201 ctacggcgag atcgctaaga ttttccgcgc tggctgcata attcgtgcgc agttcctgca
     1261 gaaaatcacc gatgcttatg cgcaaaaggc tggcattgct aacctcctgc tggcgccgta
     1321 cttcaaacag atcgctgatg actatcagca ggcgctgcgt gatgtcgtgg cgtatgcgta
     1381 tcagaatggt attccggtac cgacgttctc tgcggcaatt gcctactatg acagctaccg
     1441 ttccgcagtt ctgcccgcta acctgattca ggctcagcgt gattactttg gcgcgcacac
     1501 ttataagcgt actgataaag aaggtgtatt ccataccgag tggttggaat aatctttata
     1561 taccacagtc aggccaattg gcctgactgt tatctattgt tagttattct acaataatct
     1621 gatcaaagtc atcttatttg cacgcctgcg ttatttatcc agctctaata aaccaaatgc
     1681 ttatctttta agaatttgcc atatttatac ttttgtatac ctataataag ctgcttctgg
     1741 cctcagaata tatctgggtg tttaataaca gggttttaat taaaggatat taatatgttg
     1801 cttcctgtga taatggctgg cggtaccggc agtcgtctct ggccgatgtc tcgcgagctt
     1861 tacccgaaac agttcctccg gctgtacggg cagaactcca tgctgcagga aaccatcacc
     1921 cgactctcgg gccttgaaat ccatgaaccg atggtcatct gtaacgaaga gcatcgcttc
     1981 ctggtggctg aacagctgcg ccagctcaat aagctgtcga ataacattat acttgagccg
     2041 gtcgggcgca acaccgcgcc ggccatcgcc ctggcggccc ttcaggccac ccgctacggt
     2101 gacgacccgc tgatgctggt cctcgccgcc gaccatatca tcaacaatca gtcggccttc
     2161 cacgacgcca tccgcgtcgc cgagcagtat gccgatgaag gccacctggt caccttcggt
     2221 atcgtgccga acgccccgga aaccggctac ggctacatcc agcgcggcat ggccctaacc
     2281 gacagcgctc acaccccgta ccaggtggcc cgcttcgtgg agaagccaga ccgcgaccgc
     2341 gccgaggcct acctcgcctc cggggagtac tactggaaca gcggcatgtt tatgttccgc
     2401 gccaaaaaat acctctccga gctggccaaa ttccgcccgg atatcctcga agcctgccag
     2461 gccgcggtga acgccgccga caacggtagt gacttcatca gcatcccgca tgacattttc
     2521 tgcgagtgcc cggacgagtc cgtggactac gcggtgatgg agaaaaccgc cgacgcggtg
     2581 gtggtcggtc tcgatgccga ctggagcgat gtcggctcct ggtccgccct gtgggaggtc
     2641 agcccgaaag atgagcaggg caacgtcctc agcggcgacg cgtgggtgca caacagcgaa
     2701 aactgctaca tcaacagcga cgagaagctg gtggcggcca tcggcgtgga gaatctggtg
     2761 attgtcagca ccaaggacgc cgtgctggtg atgaaccgcg agcgttccca ggacgtgaag
     2821 aaggcggtcg agttcctcaa gcagaaccag cgcagcgaat acaagcgcca ccgcgagatt
     2881 taccgtccct ggggccgctg cgacgtggtg gtccagaccc cgcgcttcaa cgtcaaccgc
     2941 atcacggtga aacctggcgg tgccttctcg atgcagatgc accaccatcg cgttgagcat
     3001 tgggttattc tcgccggcac cggtcaggtg acggtcaacg gtaagcagtt cctgttgtcc
     3061 gagaaccagt ccacctttat tccgattggc gccgagcact gcctggaaaa ccctggctgt
     3121 attccgctgg aagtgctgga gatccagtcg gggtcgtacc tcggcgagga cgacattatt
     3181 cgtattaaag accagtatgg tcgttgctaa ttattttcgg gacaagatgc agaatgacac
     3241 agttaacttg ttttaaggct tatgacatcc gtggtgaact gggtgaggaa ctgaacgagg
     3301 acatcgccta ccgtatcggg cgcgcctacg gcgaatttct gaaacccggg aagatagtgg
     3361 tgggggggga tgtgcgcctc acaagcgagt cgttgaagct ggcgctggcc cgcggtctaa
     3421 tggacgccgg taccgatgtg ctggacatcg gactgagcgg cacggaagag atttacttcg
     3481 ccaccttcca cctcggggtg gacggcggca tcgaggtgac ggcgagtcac aacccgatga
     3541 actacaacgg catgaagctg gtgcgcgaga acgcgaagcc catcagcggc gacaccggcc
     3601 tgcgggatat ccagcgcctg gcggaggaga accagttccc gccggtggac ccggcacgtc
     3661 gcgggaccct gcgtcagatt tcggtgctga aggagtacgt cgaccacctg atgggctacg
     3721 tggacctggc gaacttcacc cgtccgctga agctggtggt gaactccggc aacggggcgg
     3781 cggggcacgt gattgatgag gtggagaaac gcttcgcggc ggctggggtg ccggtaacct
     3841 ttatcaaggt gcatcaccag ccggatggcc atttccctaa cggtatccct aatccgctgc
     3901 tgccggagtg ccgccaggat accgccgacg cggtgcgcga gcatcaggcc gacatgggga
     3961 ttgcctttga cggcgacttc gatcgctgct tcctgttcga tgacgaagct tcgtttatcg
     4021 aggggtatta cattgtcggc ctgctggctg aggcgttcct gcagaagcag ccgggagcga
     4081 aaatcattca cgacccgcgc ctgacgtgga atacggtaga catcgtgacc cgcaacggcg
     4141 gccagccggt gatgtcgaag acggggcatg cgttcatcaa ggagcggatg cgtcaggaag
     4201 acgctatcta cggcggggag atgagtgcgc accattactt ccgcgatttc gcctactgcg
     4261 acagcgggat gatcccgtgg ctgctggtgg cggagctgct gtgcctgaag aacagctcgc
     4321 tgaaatcgct ggtggcggac cgccaggcgg cgttcccggc gtcgggggag atcaaccgca
     4381 agctgggtaa tgcggcagag gcgatagcgc gcatccgggc gcagtatgag ccggcggctg
     4441 cacacatcga cacaacggac gggatcagta ttgaataccc tgaatggcgc tttaacctgc
     4501 gcacgtctaa caccgagccg gtggtgcgtc tgaacgttga gtccagagct gatgtggcgc
     4561 ttatgaatga aaaaacgacc gagctgttac acctgttaag cggggaataa ggtgagagat
     4621 ttactaacga cgatttatcg ttatcgggga tttatctgga gcagtgttaa acgtgatttt
     4681 caggcgcgct atcaaactag tatgctgggc gcactatggc tcgttttaca accactctct
     4741 atgattctgg tctataccct ggttttttcc gaggtgatga aggcaagaat gcccgataat
     4801 accgggtcgt ttgcctatag tatttatctc tgttccgggg tactgacctg gggattattt
     4861 actgagatgc tggataaagg tcagagcgta tttattaaca atgctaatct gatcaagaaa
     4921 ctcagttttc cgaaaatctg tctgccgatc atcgtgacgt tatcggcggt gctaaatttc
     4981 gcgattattt tcagtttgtt tctaattttt atcattgtca ccggtaactt ccccggctgg
     5041 ctctttctct cggtgatacc ggtcctgctt ttgcagatcc tgtttgccgg tgggctgggg
     5101 atgatccttg gtgtgatgaa cgtctttttc agggatgtgg ggcaactggt tggcgttgcg
     5161 ctgcaattct ggttttggtt cacacccatt gtttatgtac tgaattcatt acctgcatgg
     5221 gcaaaaaatc tgatgatgta taacccgatg actcggatca tgcaatctta tcagtccatc
     5281 ttcgcctatc atctggcccc caactggtat tcgctatggc cagtattggc tctcgccatt
     5341 attttctgcg tcatcggttt caggatgttc cgcaagcatg cggcggatat ggtggatgaa
     5401 ttataatgag ttatatcaga gtaaataatg tcggtaaggc gtatcgccag tatcactcaa
     5461 agaccgggag actgatcgaa tggttatccc ctctgaatac caaacgccat aatttgaaat
     5521 ggatcctcag tgacattaat ttcgaagtcg ctccgggcga ggctgtcggt attatcggta
     5581 tcaacggtgc aggcaagagt accctgctta aactcataac cgggacgtcc aggccgacga
     5641 ctggagaaat tgaaatctct ggacgtgtcg ctgcattact cgaattgggg atggggtttc
     5701 attctgattt cactggtcgg cagaatgttt atatgtctgg gcaactgttg gggttatcgt
     5761 cagagaaaat aactgaactg atgccgcaaa ttgaagagtt tgctgagatt ggggattata
     5821 tcgatcagcc tgtgcgcgtc tactccagtg ggatgcaagt tcgattagct tttagtgtag
     5881 cgacggctat ccgtcctgat gtgctaatta tcgatgaggc attatctgtt ggggatgcat
     5941 atttccagca taaaagcttt gagcgtattc gaaaatttcg tcaggaaggc accacgctgt
     6001 tgctggtatc ccatgataaa caagcgatcc aaagcatttg cgaccgggcc attttattga
     6061 ataaaggcca aattgaaatg gaaggtgaac ctgaagcagt gatggattat tacaatgctc
     6121 ttctggccga taaacaaaat cagtccatta aacaagttga gcataatggt aaaacgcaaa
     6181 ctgtttcagg cactggtgag gtgactatct ctgaggttca tcttctcgat gaacagggca
     6241 atgtgactga atttgtttcg gtagggcatc gtgtcagctt gcaggtcaac gttgaggtca
     6301 aggacgatat tcctgagctt gttgtcggat atatgattaa ggatcgactt gggcagccga
     6361 ttttcgggac caatacgtac catctcaatc agacactcac ctccctgaaa aaaggagaaa
     6421 agcgttcgtt cttattttct ttcgatgcga gattgggggt tggctcctat tctgtcgctg
     6481 tcgcgttgca tacttccagt acgcacctcg gcaaaaacta tgaatggcgc gatctggccg
     6541 tggtattcaa cgtcgttaac acggaacaac aagagtttgt cggcgtgtcc tggttgccgc
     6601 ctgaactgga gatttcttaa tgggttcgtc gttttatcgt tcatttgaag aacgacacag
     6661 aggttcggtt gaagaaatca agcgccgctt gagtttttat ttaccttttc ttgcaggtct
     6721 gaaggacatt tatcctgatg gcgtgattgc ggatattggt tgcggacgtg gcgaatggtt
     6781 ggagatcctg aatgaaaatg gcattgtgaa catcggcgtc gatctcgatg atggcatgct
     6841 ggcgcgcgcc agggaggccg gactgaatgt gcagaaaatg gattgtctgc agtttttgca
     6901 aagccaggcg gatcagagcc tgatagcgtt gaccggtttt catattgctg agcatttgcc
     6961 ttttgaggtc ctgcagcagc tcgtcatgca taccctacgg gtgctgaaac caggtggttt
     7021 gctgatcctc gaaacgccga acccggagaa tgtaagcgtc ggcacctgtt cattttatat
     7081 ggatccaacg cataatcatc ctctgccacc accactgctt gagtttttac ctattcatta
     7141 tggttttaat cgagcaatta ccgttcgtct gcaggagaaa gaggttcttc aatctccgga
     7201 tgcagccgtt aatttggtcg atgtactcaa aggggtgagc cccgactaca gcatcattgc
     7261 tcagaaagca gcgccaacag atattcttga acgctttgac accctgttta cccagcagta
     7321 cggtctgacg ctggatgctc tgagcaaccg ttacgatgcg attttgcgcc aacagttttc
     7381 gtccgttgtc tcacggctgg agacgttgaa ccaaacctat atgcaacaga taagccaaat
     7441 gtcagagact attcagacgt tgcaaggtga ggttgacgat ctgagtcatg tcatcgatca
     7501 gaaccatcag cttcatcagc aaatggcgga tttacataac agtcgttcat ggcgtattac
     7561 tcaaccacta cgctggttgt ctttgcaacg tcaattatta cgtcaggaag gggctaaagt
     7621 gcgagcccgt agggctgcga aaaaaatatt gcgcaaaggg atggcgctct cgctggtctt
     7681 tttccatcgt taccctaagt ctaaggttta tctgtttaag gttctgagaa aaactggctg
     7741 ctatacattg ctacaacgtt tgttccaacg cgtaatgctg gtgcaatctg acacgatgat
     7801 gatgcagtcc agaagatatg atgtgggtac tgaagaaatg acaagtcgcg cgatgagtat
     7861 ttataacgaa ttaaaaaata aaaatacgga gaaataacga tgcgtattgt catagattta
     7921 caaggcgcac agacggaaag ccgctttcgt ggcatcggtc gttatagtat cgcaatcgcc
     7981 agaggcataa tcagaaataa cagccggcat gagattttca tcgcgctatc cgccatgctg
     8041 gatgagtcga ttgcggatat taaggcgcaa tttgccgatc tcctgccggc agaaaatata
     8101 gtcgtatggc atgccgtagg ccctgttcgt gcgatggacc aaggtaatga atggcgtcgg
     8161 gagagcgcag aactgattcg ggaagcgttt cttgaatcat tgtgtccaga tgtcgttttc
     8221 attacgagct tgtttgaagg tcatgtcgac gatgcggcta catcggtaca caaatttagt
     8281 cgtcagtata aagtagccgt actgcaccac gatcttatcc ccctcgtgca ggcggaaacc
     8341 tatctgcagg acgatgtata caaaccctac tacttacaga aagttgagtg gttaaaaaac
     8401 gctgaccttt tgttgactaa ctctgcttat accgcacagg aagcgatcga gcatctgcat
     8461 ttacagggcg atcatgtgca gaatattgca gccgcagtcg attctcagtt ttgtatggcg
     8521 gaggtgacag cgagcgaaaa agagaccgtc cttggccatt acggtattca gcgcgagttc
     8581 atgttgtatg cgcccggagg atttgactca aggaaaaact ttaaacggtt gattgaggcc
     8641 tatgccgggc tcagtgatgc cttacgtcgc agtcatcaac tggttatcgt cagtaagctt
     8701 tccatcggtg atcgtcagta tctggaatcc cttgcgtcag gtaatggttt acagcaaggc
     8761 gaactggtac tcactggtta tgtgccggaa gatgagctga tccagctcta tcgcctatgt
     8821 aagctgttca tctttgcttc actacatgaa ggttttgggt tgccggttct ggaagcaatg
     8881 tcgtgcggtg cgccggtgat tggctcaaat gtcaccagta ttcctgaagt catcggtaat
     8941 cctgaggcat tattcgaccc gtattctgtc tcttccatga gggataagat cgcgcaatgt
     9001 ttgactgatg atactttcct cgcgcgtctg aaagacatgg cgcagcagca agcgcgaaat
     9061 ttctcttggg ataaagctgc ggtgactgct ttggaagctt tcgaaaagat cgcggtagaa
     9121 gacaccggta ctgcgcaggt tttgcctgaa gctttgattc agaaaatcct tgctatctca
     9181 caagggcagc cagatgaccg cgatctgcgc ttgtgcgcaa cggccattga ttacaatctg
     9241 aaaacggcag aactttatca aatcgacgat aaatcgctga actggcgtgt agaaggccca
     9301 ttcggatagc tcatatagtc tggcgttggt caaccgcgaa tttgcccggg cactctcagc
     9361 cgatggtgta gaggtcttat tgcattccac tgaaggaccg ggtgattttg ccccagatgc
     9421 ctcgtttatg gcgcagtcgg aaaatagtga tcttctggca ttttataatc aatgtcagac
     9481 ccgcaagagt aacgaaaaga tagatattct tagcagaaat atctatccac cgcgggttac
     9541 caaaatggat gccaaagtaa aattccttca ttgttatgct tgggaagaaa cgggctttcc
     9601 gcaaccgtgg atcaatgaat ttaatcggga acttgacgga gtgctgtgta cttcggaaca
     9661 tgttcgtaaa atattgattg ataacggact gaatgtgccc gcatttgttg ttggcaatgg
     9721 ctgtgaccat tggctcaata tcccagccga gacgacaaaa gatgtggatc acggaacatt
     9781 ccgtttcctt catgtctctt cttgtttccc tcgcaaaggg atacaggcaa tgcttcaggc
     9841 ttgggggagg gcgttcactc gtcgtgacaa tgttatctta atcattaaga cttttaacaa
     9901 tccgcacaat gaaattgacg catggctggc tcaggcccag gctcaattca tagactatcc
     9961 caaagttgaa gtgatcaaag aggatatgtc agccaccgag cttaaagggc tttatgaaag
    10021 ctgtgatgtt ttggttgctc caggttgcgc tgaaggcttt ggtttaccta ttgctgaagc
    10081 aatgctgagt gggctaccgg ctatcgtcac caattggagc gggcaacttg attttgttaa
    10141 ttcacaaaat tcatggctgg ttgactatca gttcactcgg gtaaaaacgc actttggtct
    10201 gttttcctca gcctgggcca gtgtggatat tgacaactta atagatgcat taaaagcggc
    10261 agcctcaacc gataaatcag tgctgcgtga catggccgat gctggtcgcg agcttcttct
    10321 gcagcagttt acctggaaag cggtggctga tcgttcttgc cgggcggtca agactctgcg
    10381 tgggcatatt gatattgcac agcatcgggc gcgcattggc tggttgacga cctggaacac
    10441 gaaatgtggg atcgcaacct attcccagca tctggtggag agcgcacctc atggcgcgga
    10501 tgttgttttt gctccccagg tcagcgctgg cgatcttgtg tgtgcagacg aagagtttgt
    10561 acttcgcaac tggattgtag gtaaagagag caactatctg gaaaacctcc agccacacat
    10621 tgatgctctg agactcgatg tcatcgtgat ccaattcaac tatggattct ttaatcatcg
    10681 agaactgtcg gcgtttattc gtcgccagca tgacgccggt cgttcagttg ttatgacgat
    10741 gcactcaact gtggatccgc tggaaaaaga gccgagctgg aatttccgtc ttgctgaaat
    10801 gaaagaggcg ctggcacttt gcgaccggtt gttggtgcat tcgattgccg atatgaaccg
    10861 ccttaaagat ttaggcttaa ctgcgaatgt tgctttattc ccgcacggcg ttatcaacta
    10921 ctccgcagcg agcgttacgc gtcaacagca gtctttaccg ctaattgcga gctatggctt
    10981 ctgcttaccg cataagggcc tgatggaact agtagaatcc gtccatagac tcaagcaagc
    11041 cggtaaaccg gttcgtttac gactggtgaa cgcagagtat cctgttgggg agtcacgcga
    11101 tctggtggca gagcttaaag cggctgctca gcggttaggt gttaccgatc tgattgatat
    11161 gcataatgat ttcctacctg atgcggagag tctgcggttg ctttcagaag ccgatcttct
    11221 gatttttgct tatcagaata ctggggagtc tgctagcggg gcggtacgtt atggtatggc
    11281 gactcaaaaa cctgttgcgg taacgcccct ggcgatattt gatgatttgg acgatgccgt
    11341 ctttaaattt gatggatgca gcgtcgatga tatcagtcag gggattgacc ggatcctgaa
    11401 ttccatccgt gaacaggacg cttgggcaac caggactcaa caacgtgccg atgcatggcg
    11461 ggaacaacat gattatcaag ctgtttcacg ccgtctggtt aatatgtgtc aaggcttagc
    11521 taaagctaaa tattttaaat aaaaaaatcg ctcttgtatt ttttgccttt gaatacaaga
    11581 ggggttagat aatgtgtcat ttattatgaa aattattttt gctactgagc caattaaata
    11641 cccattaacg ggcatcggtc ggtattccct ggagctggtt aagcggctgg cggtcgcccg
    11701 cgaaattgaa gaattaaagc tatttcacgg tgcgtcgttt atcgaacaga tccctttggt
    11761 ggagaataaa agcgatacca aagccagcaa tcatggtcgt ctgtcggcgt ttctacgccg
    11821 acagacgctg ttgattgagg cttatcgctt gctgcatccg cggcgccagg cgtgggcatt
    11881 gcgcgactat aaggattata tctaccatgg ccccaatttt tatctgccgc ataaactgga
    11941 acgcgccgtg accacgtttc atgacatatc catttttacc tgcccggaat atcatccaaa
    12001 agatcgggtt cgctatatgg agaagtccct gcatgagagt ctggattcgg caaagctgat
    12061 cctgactgtt tctgatttct cgcgcagtga aattatccgc ttgttcaact atccggcgga
    12121 gcggatcgta accaccaagc tagcctgcag cagtgactat atcccacgca gcccggcaga
    12181 gtgtctgccg gtactgcaga aatatcagct ggcgtggcag gcctacgcgc tatatatcgg
    12241 caccatggag ccacgtaaaa atatccgtgg cctgctgcat gcctatcagc tgctaccgat
    12301 ggagatccgc atgcgctatc cgctaatcct tagcggctat cgcggctggg aagacgatgt
    12361 gctgtggcag ttagtcgagc gcggtactcg ggaaggctgg atccgttatc tcggatatgt
    12421 tccggatgaa gacctgccgt atctgtatgc agcggccaga gtctttgttt atccctcctt
    12481 ctacgaggga ttcggtttac ctattcttga agcgatgtct tgcggtgtgc cggtagtatg
    12541 ctccaatgtc acctctttgc ctgaggttgt tggcgatgcc ggcctcgttg ccgatcctaa
    12601 tgatatagac gcgattagcg cacaaatttt gcagagcctg caggatgata gctggcggga
    12661 aatcgccacc gcgcgcggtc ttgctcaggc gaaacagttt tcgtgggaga actgtgcgac
    12721 ccagaccatt aacgcctata aattactcta agggtgtcag ttgagagttc tacacgtcta
    12781 taagacttac tatcccgata cctacggcgg tattgagcag gtcatttatc agctaagtca
    12841 gggctgcgcc cgccggggaa tcgcagccga tgttttcact tttagcccgg acaaagatac
    12901 aggtcctgtc gcttacgaag atcatcgggt catttataat aaacagcttt ttgaaattgc
    12961 ctccacgccg ttttcgctga aagcgttaaa gcgttttaag ctgattaaag atgactacga
    13021 tatcatcaac taccattttc cgtttccctt tatggatatg ctgcatcttt cggcgcggcc
    13081 tgacgccagg actgtggtga cctatcactc tgatatagtg aaacaaaaac ggttaatgaa
    13141 gctgtaccag ccgctgcagg agcgatttct cagcggcgta gattgcatcg ttgcctcgtc
    13201 gcccaattac gtggcttcca gccagaccct gaaaaaatat ctggataaaa cggtggtgat
    13261 cccgtttggt ctggagcagc aggacgtgca gcacgatccg cagagggtcg cgcactggcg
    13321 ggaaactgtc ggcgataagt tctttctctt cgtcggcact ttccgctatt acaaagggct
    13381 gcatattctg atggatgccg ctgagtgtag ccgactgcca gtggtggttg tagggggcgg
    13441 gccgctggaa tcggaagtgc ggcgtgaagc gcagcagcgc gggctgagca atgtgatgtt
    13501 taccggcatg ctcaacgacg aagataagta cattctcttc cagctctgcc ggggcgtggt
    13561 attcccctcg cacctgcgct ctgaggcgtt tggcattacg ttattggaag gcgcacgctt
    13621 cgcaaggcca ctgatctctt gcgagattgg tacaggtacc tctttcatta accaggacaa
    13681 agttagtggt tgcgtgattc cgccgaatga tagccaggcg ctagtggagg cgatgaatga
    13741 gctctggaat aacgaggaaa cctccaaccg ctatggcgaa aactcgcgtc gtcgttttga
    13801 agagatgttt actgccgacc atatgattga cgcctatgtc aatctctaca ctacattgct
    13861 ggaaagcaaa tcctgagaga tgctaccccc gccgttgctg cgggggccaa tgcgttaatg
    13921 ccgattcttc agattatcaa tcacttctcc gagatccagc ccctgatcct gtaacagcac
    13981 cagcaggtga tacatcaaat cagatgcctc gttggtcagc tcaaagcggt catgtaccgt
    14041 tgctgccagt gcggtttcca cgccttcttc gcccactttt tgcgcaatgc gtttggtgcc
    14101 gctggcatac agtttggcag tgtaagaggt ttccgggtcg gcagatttgc gttcggcgag
    14161 caattgttcc agttgataca ggaacagcca ttggtgagcg gtgtcgccga agcagctgct
    14221 ggtgcctttg tggcaggtcg gaccgatggg attcgccagc actaataaag tgtcgttgtc
    14281 gcagtctggt gcaatactaa ccacattgag gaaattgccc gacgtttcgc ctttggtcca
    14341 cagtcgctgt ttagtgcgcg agaagaaagt gactttgccg ctttcgaggg ttttgtctaa
    14401 ggcttccggg ttcatatagc caagcattag cacttcgccg gataccgcgt gttgcacaat
    14461 caccggcatc agtccgtcgg ttttttccca gtccagttcg cgacgttgtt gttctgttaa
    14521 catatcctga tctccacgcc ctgtgttgcc aggtacgctt ttaattcacc aatattgatt
    14581 atttgtttgt ggaatacaga agctgccagc gcgccgtcaa cgtcggcatc gcggaaggct
    14641 tcgaggaagt gttccatggt gcccgcgcca ccggaggcaa tcagcgggac gtggcaaact
    14701 tcacgcactt ttttcagttg ttccaggtcg taaccgttac gcacgccgtc ctgattcatc
    14761 atgttgagaa cgatttctcc ggctccacat ttttgcactt cctgtaccca gtcgagtgtt
    14821 tcccactgag tgacgcgggt gcgactttca tcgccggtat attgattcac atgatattta
    14881 ccggtttcag cgtcgtacca ggtatcaata ccgaccacaa tacactgcac gccaaagcga
    14941 tccgccaggc gagtaattaa cgtcgggtcc gccagcgcag gagagttgat ggaaatttta
    15001 tccgcgccaa aggaaagaat tttcgcggca tcttccagag acttaatccc acccgccaca
    15061 cagaacggaa tgtcgatcac ttccgccacg cgagataccc agcttttatc taccacacgg
    15121 ccatcgctgg aagcggtgat atcgtagaac accagttcgt ctgcaccttc ttcagcgtag
    15181 cgttttgcca gcggtacaat atcgccaatg atttcatggt tgcgaaactg tacgcctttc
    15241 accacctgac catcacgaac gtcgagacat gggattatgc gttttgccag catgc
//
