LOCUS       AB812034               15306 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: H14.
ACCESSION   AB812034
VERSION     AB812034.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 15306)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..15306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H14"
                     /db_xref="taxon:562"
                     /note="serogroup:O82"
     gene            <1..885
                     /gene="wcaM"
     CDS             <1..885
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01260.1"
                     /translation="GFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIER
                     INCTNDKINWGIGIGLSGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVI
                     RNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSI
                     PQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMR
                     NINVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRIN
                     HHIVNVEKINFRLPERRE"
     gene            1048..1953
                     /gene="galF"
     CDS             1048..1953
                     /gene="galF"
                     /note="O082_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01261.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     CDS             2476..3999
                     /note="O082_02"
                     /gene="asnB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative asparagine synthetase"
                     /protein_id="BAQ01262.1"
                     /translation="MCGFFITNNPSINSSHLKLVEDSLAFRGPDFSSGFINTNGWIVY
                     HSRLSIIDLTEDANQPIFDSKGGVLVFNGEILNFKELGIKYFNKDFESDSKLLSALLE
                     RKLIDLNEFDGFFSFVYVNHNGKLEYAVRDKFGVKPLFYHKSEEGYLSFSSEPSLLNR
                     LYNCGLNKDAIDEYYSIRYPIFSGSYFNDVFCINPGECYINGEYFNILNYFSGKYVET
                     NINEIELSIKKGIKTRKVSDAPVGLLLSKGIDSNLLNTLSEFNFKYSIGFKGDADLEY
                     LKQQNIKNLKIVECNNQLYKDAFDYLIKLRGEPMSVPNEVLLYLIAKEAASDGVKVLL
                     SGEGADEFFGGYDRIFTWALYAQEFVLEDFIEHYCYELPAENTKLVYKFKSIFEQVSH
                     LTPFEKVRWFFIRYHMPILFRRLDFSLMAAGIEGREPLANIHTFMQAIKVSPSQLMTD
                     GLGKIPLRNIIAKYMGKDFAFSSKVGFPVDLRKVFDYTGNETSYEIWFKENLKGVGI"
     CDS             3996..4415
                     /note="O082_03"
                     /gene="wbeS"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycerol-3-phosphate
                     cytidyltransferase"
                     /protein_id="BAQ01263.1"
                     /translation="MIIGYTTGVFDLFHIGHVNLLKNAKSLCDKLIVGVTIDELVLYK
                     GKRSVIPFHERIQVVQACKYVDVALPQSDMDKLTAVKKYHANILFVGDDWYNTEKWCV
                     YEKELAEIGCKIIYFPYTQGTSSTLINDTLKKLRNNQ"
     gene            4475..5704
                     /gene="wzx"
     CDS             4475..5704
                     /gene="wzx"
                     /note="O082_04"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01264.1"
                     /translation="MFKHSVFINATALFFMQLLGYISPLLVYPYLTRVLGISGFGQYA
                     MAISITALSFIITDFGFALSGANWLAKNRNKTKEVKHYLSNVCLLKFIFFALCSVVSS
                     LVIITSQQLSYLLHSIPAIIFLLFSQTFQMNWLFQGLEKMSLAIYPFVISRIIFILTV
                     FIFVKYKADVNIAIFLLGFSNLLSVIISACLLAREGLSFCKPSFRDSFNILKNNISFF
                     LSRASVSIYTSASAFILGYTTNVHAVAYYSAAEKLYTAGQSLTGTVSQALYPYLSRTG
                     NKNIFNIALIIVMLPLTIGIGIAFIFSSELINLIYGTAYSESIPILKIFLICLIINFI
                     GVSYGYPAFAIINRIDVANKTVYMGGLFQISSLLYLYVNDIISANSVAISVLMTETFV
                     MCLRILFYYIFSKNAHD"
     CDS             5697..6875
                     /note="O082_05"
                     /gene="wbeZ"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycerophosphotransferase"
                     /protein_id="BAQ01265.1"
                     /translation="MIKKLKLKIYALSSYLLGHFLNADDDLWIIGAGNGYVFGDNSRY
                     FYQWVIEKKNKKIFWVTKDLKIYHALSKNEKTKDRVIYFYSLKSILFIARAKVFICSH
                     GVNDITPYVCKNRKLICLWHGLPIKKIGDLTLSSYGKDNPLERGIKHVINSKKEYDVF
                     LSPSLYYKEIFEKSFEGKISEFIYAQYPRVTELKNKSNDIADNSDIKTILYAPTFRDF
                     VAPSYYEINGIVPSKKLLSELNCILGYNRYKLIIKLHPYICIENSYMHELIGFNNIIF
                     ANKNDDVLDLLINASVLITDYSSICFDFLTLGKEIIFLMPDYDQYVGQTRGLNFELKD
                     FAPGKIFTNWNDLIKYIPVINRKFTLDDDYLKALKMSNEFHTRKNSYIFDFIEKRAKL
                     "
     gene            6875..7957
                     /gene="wzy"
     CDS             6875..7957
                     /gene="wzy"
                     /note="O082_06"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01266.1"
                     /translation="MVKEFKIKTDCYIIILWFFIAAFKILFNNILYFRDVEVIQFIIT
                     ILFWLIGLVIILRRFLNHAIKLDTLLIVCSVLICLSILNANLLTFVFAVLVWSSISYL
                     LDRRAFIFFYSASLFFLIITILISVALNEQVFYIDGRYGKVPTFGFENSNSFPQLLVI
                     YLLISTSRLRTSIILGSIILFFAYMELKTRSMYMIIILYPLVILLLKFFKGKIICILP
                     LVLIVLNFVMVLNLNNEYIKSLDVLLSYRLSYSLELINNLSFSQWFTGTSSELGIPMD
                     MSYINLVYKYGIIATAMIVVLAMKALHVALSHSDSKVVAIIVCFFCYAFVENVLLNYY
                     LNPTLYFIFYFYAKNKQREWLNNNAG"
     CDS             7981..8670
                     /note="O082_07"
                     /gene="wffM"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01267.1"
                     /translation="MIPKIIHYIWLGDIDNMPKLSKKCIDSWKKHLLNYEIIFWDECK
                     IRSTFKENEIEFFNKMVKLKKFAFAADYARCIILDKFGGVYLDTDIEIIRDITPLLSV
                     SDVFLGLEDDNKPNCAVIGATSNNIFLKKLIKFIIQADGLTEIPILAYEALLGISERF
                     CDLNRLTIIDGIAVYPEQFFYPYNPYRRDSLSQLMFSDITEETYAIHHWAKTWKLTIF
                     ERIKKRLFKQK"
     CDS             8855..9745
                     /note="O082_08"
                     /gene="wcnV"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01268.1"
                     /translation="MSRVTIGILTYKRMDYLISLIKDLNAVKIKIDLIILNNNENIDV
                     FNDIADIVDSNFINYKYIWNQKNHGVSEGRRIIVENCNTDYLMLLDDDIYVEDINLIC
                     RKIINSFDSDKFLGAIAFNIIDFNTKKNNRYEIPHKNKKVNLNADFYTYLIIGAGLAL
                     RTSAVKDVGNFSNTLGPYGFEEIDVAFRLINAGYKIKYLHDCIIEHKKSPDGRFSNEM
                     VNYYALVNRTILAKKYLRKKYFISCLVIRSLFFIVKTKNIRLLFKAWAEIIKTESHAS
                     DKFKQCFYDYCKGVSGFLYY"
     CDS             10069..10809
                     /note="O082_09"
                     /gene="wfgD"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01269.1"
                     /translation="MPYVTIVMPAYNSEMYIAQAIESVINQTHTNFELLICDDGSTDG
                     TTSIVSKYIVQDNRIKLLENINPKGAAGARNTCLDYAKGDFIAFLDSDDYWVEDKLEK
                     QLNFMLENNIQFSYSNYYMFAERFQKKISCRKHNSFNSLTYTCDIGCLTVVLSKKAIG
                     ELRFPYVPKEDYAFWLMLLEQDLMAYNQGGYNAFYRKQKQSLSGNKLKEIVRQYIVLT
                     NATNLSKCAIFYRLITYGYNAFIKHYVK"
     gene            11092..12498
                     /gene="gnd"
     CDS             11092..12498
                     /gene="gnd"
                     /note="O082_10"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01270.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELSQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKDGVFHTEWLD"
     gene            12744..13910
                     /gene="ugd"
     CDS             12744..13910
                     /gene="ugd"
                     /note="O082_11"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01271.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKINFNATLDKNEAYRDAGYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVAINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQTVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            14000..15025
                     /gene="wzz"
     CDS             14000..15025
                     /gene="wzz"
                     /note="O082_12"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01272.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLLVQLW
                     RGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAP
                     KVSEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETPEGA
                     QKQLAQYIQQVDDQVNEELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQIQE
                     ALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQN
                     LLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNA
                     LRNYNAK"
     gene            complement(15121..>15306)
                     /gene="hisI"
     CDS             complement(15121..>15306)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01273.1"
                     /translation="TKRIAQKVGEEGVETALAATVHDRFELTNEASDLMYHLLVLLQD
                     QDLDLTTVIENLRKRHQ"
BASE COUNT     4805 a   2521 c   3033 g   4947 t
ORIGIN
        1 ggatttcata accagattat cggtgccaac atcaccaatt gtaagttcag cgacttacag
       61 ggggacgcta ttgaatggaa cgtggcgatt aacgacagtg atattttgat ctccgaccat
      121 gtcatcgagc gcatcaactg tactaacgac aaaatcaact ggggcatcgg cataggtctt
      181 tcgggtagta cctacgataa caactacccg gaagaccagg cagtgaaaaa ctttgtcgtg
      241 gcgaatatca cgggatcgga ttgtcggcag ttgatccatg ttgaaaatgg caaacatttt
      301 gttattcgta atatcaaagc ccgcaatatc acgccggatt tcagtaagaa agcaggtatt
      361 gataacgcga cggtcgctat ttacggttgt gacaatttcg tgattgataa tattgaaatg
      421 attaatagcg ccgggatgtt aatcggctat ggggtaatta aaggcaaata tctctcgata
      481 ccgcaaaatt tccgagtgaa taatattcaa ctggataata cccaccttgc ttacaaattg
      541 cgcggcatcc aaatctccgc cgggaatgct gtctcctttg tggcgctgac taacattgag
      601 atgaagcgtg cgtcgctgga gttacacaac aaaccgcaac atctttttat gcgtaatatt
      661 aatgtgatgc aggaatcctc tgttggaccc gcattgagca tgaacttcga catgcgcaaa
      721 gacgttcgcg gcgtctttat ggcgaaaaaa gaaacactcc tgtctcttgc aaatgttcat
      781 gcggtgaatg aaagaggaca aagctccgtc gatatcgaca ggattaatca ccatattgtt
      841 aatgtggaaa agattaactt tagattgccg gaacggaggg agtagatttg cgaccattcc
      901 tggaaaaatg gagccatact taggaacaat gctactgcaa tccacaacga agcggcgtaa
      961 catcacaagt aattcagtaa tcaattcagg gtaattgatg ctggcgaaaa aaatcgaaca
     1021 agctataatt cagcaaccat tttacaggtg gatgaaataa tgacgaattt aaaagcagtt
     1081 attcctgtag cgggtcttgg gatgcatatg ttgcctgcca ctaaggcgat tcccaaagag
     1141 atgctaccaa tcgtcgacaa gccaatgatt cagtacattg ttgacgagat tgtggctgca
     1201 gggatcaaag aaatcctcct ggtaactcac gcgtccaaga acgcggtcga aaaccacttc
     1261 gacacctctt atgaattaga atctctcctt gagcagcgcg tgaagcgtca actactggcg
     1321 gaagtacagt ccatttgccc gccgggagtg accattatga acgtgcgtca gggcgaacct
     1381 ttaggtttgg gccactccat tttatgtgca cgacccgcca ttggtgacaa cccatttgtc
     1441 gtggtgctgc cagacgttgt gatcgatgat gccagcgccg acccgctgcg ctacaacctt
     1501 gcggccatga ttgcgcgctt caacgaaacg ggccgcagcc aggtgctggc aaaacgtatg
     1561 ccgggtgacc tctctgaata ctccgttatt cagaccaaag aaccgctgga tcgcgaaggt
     1621 aaagtcagcc gcattgttga attcatcgaa aaaccggatc agccacaaac gctggattca
     1681 gacatcatgg ccgtgggccg ttatgtgctt tctgccgata tttggccgga acttgaacgc
     1741 actcagcctg gtgcatgggg gcgtattcag ctgactgatg ccattgccga actggcgaaa
     1801 aaacagtccg ttgatgccat gctgatgaca ggtgacagct acgactgcgg taaaaaaatg
     1861 ggttatatgc aggcatttgt gaagtatgga ctgcgcaacc tgaaagaagg ggcgaagttc
     1921 cgtaaaggga ttgagaagct gttaagcgaa taatgaaaat ctgaccggat gtaacggttg
     1981 ataagaaaat tataacggca gtgaagattc gtggcgaaag taatttgctg cgaatattcc
     2041 tgccgttgtt ttatataaac aatcagaata acaacgagtt agcaatagga ttttagtcaa
     2101 agttttccag gattttcctt gtttccagag cggattggta agacaattag cgtttgaatt
     2161 tttcgggttt agcgcgagtg ggtaacactc gtcacatcgt agacatgcat gcagtgctct
     2221 ggtagctgta aagccagggg cggtagcgtg tgttaatata gtcaattatt tatcaaaatg
     2281 taaataaatg tctctcaaca ttgttcgtac tacactatgt taattttgtc agataagtat
     2341 atatttacat tatattaatt tttattttta agtaacagta taattaaatt ttggtgtgaa
     2401 gttttttaat tggcatagcg ctatttatat ttcttctaat ccagtctgta attaatgttt
     2461 taaggaattt atcgtatgtg tggttttttt atcactaata atccgagtat taattcgtca
     2521 catttgaaat tagttgaaga ttcattagca tttcgaggtc ctgatttcag ttctggattt
     2581 attaatacaa atggatggat tgtttatcat tcaaggcttt cgatcatcga tttaacagaa
     2641 gatgcaaatc agccaatatt cgattctaaa ggtggtgtct tagtatttaa tggggagatt
     2701 ttaaatttta aagaattggg tataaaatat tttaataaag acttcgaaag tgattcgaag
     2761 ctattgagcg ctttactgga aaggaaactt atcgatttga atgaatttga tggttttttt
     2821 tcgtttgttt atgtaaatca caacggcaaa cttgaatatg ctgtccgcga caaatttggt
     2881 gttaaacctt tattctatca taaaagcgag gaaggatatt tatcatttag ctccgaacca
     2941 agtttgttaa atcgattata taattgtggt ttaaataaag atgcgatcga cgagtattac
     3001 tcaattagat accctatttt ttcaggaagt tattttaatg atgtcttttg tattaatccc
     3061 ggtgaatgtt atataaatgg tgaatacttt aatattctaa attatttttc aggtaaatat
     3121 gttgagacta atatcaatga aatagaatta agtataaaaa aaggaattaa aactcggaaa
     3181 gtttcagatg cacctgttgg cttgctttta agtaaaggca tagatagtaa tcttttaaat
     3241 actctttcag agtttaattt taagtatagt attggcttta aaggcgatgc agatttagaa
     3301 tacttaaagc agcaaaatat aaaaaatcta aaaattgtcg aatgtaataa tcaattatac
     3361 aaggatgcat ttgattattt gattaaatta cgtggtgaac ctatgtcagt tcctaacgaa
     3421 gttttattat atttaatagc aaaagaagca gcttcggatg gagtaaaagt attgttatca
     3481 ggagaagggg ctgatgaatt ttttgggggc tatgatcgta tttttacttg ggctttatat
     3541 gcgcaagagt ttgtattaga agattttatt gaacattact gttatgagct gccagcggaa
     3601 aatactaaat tagtgtataa atttaaatca atatttgaac aagtatctca tctaacacct
     3661 ttcgaaaaag ttcgttggtt ctttatccga taccacatgc caatattgtt tagacgcctt
     3721 gatttttcac taatggctgc aggtatagaa gggcgcgaac cattagccaa tattcataca
     3781 tttatgcagg cgatcaaagt atcaccatca cagttaatga cagacggatt gggtaaaata
     3841 ccgttacgca atattattgc taagtatatg ggaaaagact ttgctttctc aagtaaagtt
     3901 ggcttccctg ttgatttacg gaaagttttc gattatacgg gcaatgaaac ttcttatgaa
     3961 atatggttta aagaaaattt aaaaggggtt ggaatatgat tattggttat acaacagggg
     4021 tatttgatct ttttcatatt ggtcatgtaa atttgctaaa aaatgcaaag tctttatgtg
     4081 ataaattaat tgtcggggtg actatagacg aattggtact atataagggt aaaagatctg
     4141 taatcccttt tcatgaaaga atacaagttg tccaagcatg taaatatgtt gatgtggctt
     4201 taccgcaaag tgatatggac aaactaacag ctgtaaaaaa atatcatgca aatatattat
     4261 ttgttggtga tgattggtat aacactgaaa aatggtgcgt ttacgaaaaa gaattggcag
     4321 aaattggatg taaaataatc tatttccctt atactcaggg cacttcaagt acattaatta
     4381 atgatacatt aaaaaaatta agaaataatc aataaggata aggagcattt attgctcctt
     4441 aatacattaa gttattaaat ttgcaagtgc cattatgttt aaacacagtg tatttataaa
     4501 tgcaactgca ttgtttttta tgcagcttct cggttatata tcgccacttt tagtttatcc
     4561 ctatttaacc agggtgctcg ggatatctgg atttggacag tatgctatgg ccatatcaat
     4621 tactgcctta tctttcataa ttaccgattt tggatttgcc ctttcaggtg ctaattggct
     4681 ggccaaaaac agaaataaaa ctaaagaagt aaaacactat ctctcaaatg tatgtttatt
     4741 aaaatttata ttcttcgctc tttgttctgt tgtatcaagc ttggttataa ttacttcaca
     4801 acaactttca tacttgttac attcaatacc tgcaattatt tttttattgt tttcgcaaac
     4861 attccaaatg aattggctat tccaaggatt agagaaaatg tctttggcta tatatccttt
     4921 tgttatatcg aggattatct ttatattgac ggtgtttata tttgttaaat ataaagcaga
     4981 tgtcaatatt gctatttttt tattaggttt ttcaaatttg ctgtcagtta taattagtgc
     5041 ttgtctgtta gcgcgagagg gtttgagttt ctgcaagccg agttttaggg attcattcaa
     5101 tattttaaag aataatattt ctttctttct ttcaagagca tctgttagta tttatacaag
     5161 tgcaagtgca tttattttag gttatactac aaatgttcat gctgtagcat attattctgc
     5221 agcagaaaaa ttatatacag ctggtcaatc attaactgga actgtaagcc aggcgttgta
     5281 cccatatcta tcgcgaacag gaaataaaaa tatattcaat atagccttga taattgttat
     5341 gcttcctttg acaataggaa taggaattgc ttttattttc tcatcagaat taattaattt
     5401 gatttatggt acagcctatt ctgaatcaat ccctatctta aagatttttt tgatttgttt
     5461 aatcattaat ttcatcggcg tatcatatgg ttatccagct tttgcaatta taaatagaat
     5521 agatgttgca aacaagacag tatatatggg ggggctattt caaataagtt cattattata
     5581 tctctatgta aatgatataa tctctgcaaa cagtgttgct ataagtgttc tcatgactga
     5641 aacgttcgtg atgtgtttga gaattttatt ctattatatc ttttcaaaga atgcacatga
     5701 ttaaaaaatt aaagcttaag atttatgcac tttcttcata tttgctagga cattttctaa
     5761 atgcagatga cgacttatgg attattggtg caggtaatgg gtatgtattt ggggataatt
     5821 cgcgatattt ttatcagtgg gttatcgaga aaaaaaataa aaaaatattt tgggttacaa
     5881 aagatctaaa aatatatcat gcactatcca aaaatgagaa aacgaaagat agagtgattt
     5941 atttttattc gctgaaaagt atactattta ttgcgcgcgc aaaagttttt atttgcagtc
     6001 atggtgtcaa tgatataaca ccatatgttt gtaaaaatag aaaattgata tgtttgtggc
     6061 atggtttgcc tataaaaaaa ataggtgatt taacactaag ttcatatgga aaagataatc
     6121 ctcttgaacg gggcataaaa catgtgatta attcaaaaaa agagtatgat gtatttctaa
     6181 gtccatctct ttattataaa gaaatatttg aaaaatcttt tgaaggtaaa atatctgaat
     6241 ttatatatgc acaatatcct cgtgtaacag agctcaaaaa taaatcgaat gatatcgctg
     6301 ataatagtga tataaagaca attttatatg caccaacatt tagagatttt gtcgcccctt
     6361 cttattatga aataaatgga atagttccat caaagaaact attaagcgaa ttaaattgta
     6421 tcttgggata taatagatat aagttaataa taaaactaca tccctatatt tgtatagaaa
     6481 attcatacat gcatgaatta attggtttca ataatattat atttgcaaat aagaatgatg
     6541 atgttctaga cctgctgatt aatgccagtg tattaatcac agactattct agtatttgtt
     6601 ttgatttctt aacgttggga aaagaaatca ttttcctaat gcctgattat gaccaatacg
     6661 ttggtcaaac cagaggttta aactttgaat taaaagattt tgctccagga aagattttca
     6721 ctaattggaa tgatttaata aagtatatcc ctgtgattaa tcgcaaattt actttggatg
     6781 atgattatct aaaagctttg aagatgtcaa acgaatttca cacgagaaaa aattcctata
     6841 tttttgattt tatagaaaaa agagcaaagc tataatggtg aaagagttta aaattaaaac
     6901 tgattgctat attattatac tatggttttt tattgctgcc tttaagatct tatttaacaa
     6961 tatattatat ttccgtgatg ttgaagtaat acaattcata attacaattt tattttggct
     7021 gattggcctg gttattatcc ttagaagatt tttgaatcat gctataaaat tagacacatt
     7081 attaattgtt tgttctgttt taatatgctt gtcaatattg aatgcgaatc ttttaacgtt
     7141 tgtttttgct gtattggtat ggagctccat ttcttatctc ctggatagaa gggcgtttat
     7201 ttttttttac tctgccagtt tgtttttttt aattataact atactaataa gtgtggccct
     7261 caatgagcaa gtgttttaca tcgatggtcg ttacggaaaa gttcctacgt ttggcttcga
     7321 aaattctaac agttttccgc agttattagt aatatattta ttgatatcta cttcacgtct
     7381 acggacctct ataatcttgg gtagtattat cttgtttttt gcttatatgg aattaaaaac
     7441 aaggtctatg tacatgatta ttattcttta tccattagtg attttattat taaagttttt
     7501 caaaggtaaa atcatttgta ttcttcctct ggttttgatc gttttaaatt ttgtgatggt
     7561 tcttaattta aacaatgaat acataaaaag tttggatgtg cttttatcat atagattatc
     7621 ttattctttg gagctaatta ataatctatc attctctcaa tggtttactg ggacttcatc
     7681 ggaacttggt atcccaatgg atatgtcata catcaactta gtttataaat atggaataat
     7741 agcaactgca atgattgtcg ttttagcgat gaaagccttg catgttgctt taagtcatag
     7801 tgatagtaag gttgtagcaa tcatagtatg tttcttttgt tatgcgtttg tagagaatgt
     7861 acttctgaat tattatctaa atcccacatt atattttatc ttttattttt acgcaaaaaa
     7921 caaacagcgt gaatggttga ataataatgc tggataacat tttaaaggtg tatgatagaa
     7981 atgataccta aaataataca ttatatatgg cttggtgata ttgataatat gccaaaacta
     8041 agcaaaaaat gtatagatag ttggaaaaaa catcttttaa attatgaaat tatattttgg
     8101 gatgaatgca aaattagaag cacatttaaa gagaatgaaa ttgagttctt taataaaatg
     8161 gttaagctaa aaaaatttgc atttgctgct gattatgcaa gatgtattat ccttgataaa
     8221 tttggtggag tgtatttaga taccgatata gaaataatac gtgatattac tccgttactt
     8281 tcagtcagtg atgtattcct cggattagaa gacgataata aaccaaattg tgctgttatt
     8341 ggtgccacta gcaataatat ttttttgaaa aaattaataa agtttataat tcaagctgat
     8401 ggtctcacag aaattcctat attagcgtac gaagcattgc taggtatatc tgaaagattt
     8461 tgtgatttaa acaggctcac aataattgat ggtatagcag tatatccgga gcagttcttt
     8521 tatccttata atccttatcg cagagattca cttagtcaat tgatgtttag cgatattaca
     8581 gaagaaacat acgcaataca tcattgggct aaaacttgga aactaactat ttttgagcgt
     8641 ataaaaaaaa gattgtttaa gcagaaataa aaataagctg tattattata ttatagttta
     8701 aggttattat tataaatatc tattagcaca tggtaaatac agttatgttt tagtcgtatt
     8761 aatgttttct attgtatgag ttgatgagta tgtttattag attgctacaa ttgaattaat
     8821 cgaacaatat gattggacag aaggagtgat ttaaatgtcg cgggttacaa ttggtatttt
     8881 gacatacaaa cgaatggatt atcttatatc attgataaaa gatctaaatg cggtaaagat
     8941 taaaatagac ttgattatat taaataacaa tgaaaatata gacgttttta atgatattgc
     9001 agatattgtg gattcaaatt ttattaatta taaatatatt tggaaccaaa aaaatcatgg
     9061 tgtatcagaa ggccgccgga ttatagttga aaattgtaat acagactatt taatgctatt
     9121 agatgatgat atctatgtcg aagatattaa tttaatttgt aggaaaataa ttaattcatt
     9181 cgatagtgat aaattcttag gtgctattgc ttttaatatt attgatttca ataccaaaaa
     9241 gaataataga tatgaaatac cacataagaa taaaaaagta aacttaaatg ctgattttta
     9301 cacttatctt attattggtg ctggcttagc gcttcgaact tcagccgtaa aagatgtggg
     9361 gaatttctct aatacattag ggccttatgg tttcgaagaa atagatgttg cattccggtt
     9421 aataaatgct ggatacaaaa taaaatattt gcatgattgc ataattgaac ataaaaaatc
     9481 accggatgga cgtttttcta atgagatggt caattattac gccttagtta acaggacgat
     9541 tttagcaaag aaatatctga ggaaaaaata ttttatttct tgtctagtaa ttagatcatt
     9601 atttttcata gtaaaaacta aaaatataag attgctattc aaagcctggg cggaaattat
     9661 aaaaactgaa agtcatgcaa gtgataaatt caaacaatgt ttttatgatt attgcaaagg
     9721 cgttagtggt tttttatatt attgaagtat gattggaatc ggctttgtta aatgcatctt
     9781 taagataata tgatctttag aatatatttt tgtttatatt gtgtgttaat ttaaacatct
     9841 atagtttgaa gaaataactt ggccaggcaa atatgggttt ttcttgagta ttaatatgta
     9901 aatgtaattt tctaatatgt tttattctta tcggtgatat tcttcgttaa attcaaaatg
     9961 tgcattttta taagattatg ggttattgtt aagtgtttga aaacatttta agagggtaag
    10021 tctaataaaa ttcataatca cctatttaat aagggtaata aatattctat gccatatgtg
    10081 acaatagtaa tgccagctta taattctgaa atgtatatag cacaggcaat agaatctgtt
    10141 ataaatcaaa ctcatactaa tttcgaattg ttgatctgtg atgacggttc aacggatggc
    10201 acaactagca ttgtttcaaa atatatagtt caggataaca ggattaagtt attggaaaat
    10261 attaacccta agggggcggc aggtgctaga aatacctgtt tagattatgc taaaggagac
    10321 tttattgctt ttttagatag tgatgattat tgggttgaag ataaattaga aaagcaatta
    10381 aattttatgt tagaaaataa catacaattt agttattcaa attattatat gtttgctgaa
    10441 cgattccaaa aaaaaatatc atgtagaaag cacaattctt ttaactctct aacgtacacc
    10501 tgtgacattg gctgtttgac agttgtttta agtaagaaag ctatcggtga attaagattt
    10561 ccatacgtgc ccaaagaaga ttatgcattt tggttaatgt tattagaaca agatttaatg
    10621 gcttataatc aaggtggata caatgctttt tatcgaaaac agaaacaaag tttatctgga
    10681 aacaaactta aagagatagt tagacaatat attgttttga caaacgctac taacctatct
    10741 aagtgtgcta ttttttatag attaattact tatggatata atgcgtttat aaaacactat
    10801 gttaaatagt ttttaaataa aataaaccta taacaagtcg tgatatttaa atgattgcac
    10861 aaataattga ttaatattta catgattcta ttgtcattat aaaaagtcat ttgtgatatg
    10921 gggtatggta atatataata tttagctgta gtacaaaatt tgttgttaat tatttaatca
    10981 atatgagaac ctatccaaac tttgttcact gagcaacttc actttctaag ttaatttatt
    11041 aatcacagcg aaatctcata atcatgtggt aacccctgac aggagtaaac aatgtcaaag
    11101 caacagatcg gcgtcgtcgg tatggcagtg atggggcgca accttgcgct caacatcgaa
    11161 agccgtggtt ataccgtctc tattttcaac cgttcccgtg aaaagacgga agaagtgatt
    11221 gccgaaaatc cgggcaagaa actggttcct tactatacgg tgaaagagtt tgttgaatct
    11281 ctggaaacgc ctcgtcgcat cctgttaatg gtgaaagcag gtgcaggcac ggatgctgct
    11341 attgattctc tcaagccata cctcgataaa ggcgacatca tcattgatgg tggtaacacc
    11401 ttcttccagg acaccatccg tcgtaaccgt gagctttctg cagaaggctt taacttcatc
    11461 ggtaccggtg tctccggcgg tgaagaaggt gcgctgaaag gtccttccat tatgcctggt
    11521 gggcagaaag aagcctatga actggttgcg ccgatcctga ccaaaatcgc cgcagtggct
    11581 gaagatggcg aaccgtgcgt tacctatatt ggtgccgatg gtgcaggtca ttatgtgaag
    11641 atggttcaca acggtattga atacggtgat atgcagctga ttgccgaagc ctattctctg
    11701 ctaaaaggtg gcctgaacct taccaacgaa gaactgtcac agacctttac cgaatggaat
    11761 aacggtgaac tgagcagcta cctgatcgac atcaccaaag atatcttcac caaaaaagat
    11821 gaagacggta actacctggt tgatgtgatt ctggatgaag cagcaaacaa aggtacgggt
    11881 aaatggacca gccagagtgc gctggatctc ggtgaaccgc tgtcgctgat taccgagtct
    11941 gtgtttgcac gttatatctc ttctctgaaa gatcagcgtg ttgccgcatc taaagttctc
    12001 tctggcccgc aagcgcagcc agctggcgac aaggctgagt tcatcgaaaa agttcgtcgt
    12061 gcgctgtatc tgggcaaaat cgtttcttac gcccagggct tctctcagct gcgtgctgcg
    12121 tctgaagagt acaactggga tctgaactac ggcgaaatcg cgaagatttt ccgtgctggc
    12181 tgcatcatcc gtgcgcagtt cctgcagaaa atcaccgatg cttatgccga aaatccacag
    12241 atcgctaacc tgctgctggc tccgtacttc aagcaaattg ccgatgacta ccagcaggcg
    12301 ctgcgcgatg tcgtcgcata tgcagtacag aacggtatcc cggttccgac cttcgccgct
    12361 gcggttgcct attatgacag ctaccgtgcc gctgttctgc ctgcgaacct gatccaggca
    12421 cagcgtgact atttcggggc gcatacttat aagcgcattg ataaagacgg tgtgttccat
    12481 actgagtggc tggattaatc tgatttaccc agcaatattt tgaggcccgg catatattgc
    12541 cgggcttttt tttgccaatt atcttataga ctaaatttca ctgcttaata ttaacttaat
    12601 aaatatcagc tattcttata aagaaaatct gaattgtttt tcgttgcgtt gaccatcgaa
    12661 gaacgtagcg ttaaaacttt tagctcttat caggatgcta aaaacatcat gattcatagt
    12721 taagttaatt ctgagagcat gaaatgaaaa ttaccatttc cggtactggc tatgtaggct
    12781 tgtcaaacgg gcttctaatc gcacaaaatc atgaggttgt ggcattagat attttaccgt
    12841 cacgcgttgc tatgctgaat gatcggatat ctcctattgt tgataaggaa attcagcagt
    12901 ttttgcaatc agataaaata aactttaatg ccacattaga taaaaatgaa gcctaccggg
    12961 atgctggtta tgtcatcatc gctactccaa ccgactatga tcctaaaact aattatttca
    13021 atacatccag tgtagaatca gtaattaaag acgtagtagc gataaatcct tatgcggtta
    13081 tggtcatcaa atcaacggtt cccgttggtt ttaccgcagc gatgcataag aaatatcgta
    13141 ctgaaaatat tattttctct ccggaattcc tccgcgaagg taaagccctt tacgataatc
    13201 tccatccttc acgtattgtc attggtgagc gttcagaacg cgcagaacgt tttgcggcgt
    13261 tattacagga aggggcgatt aagcaaaata tcccgaccct gttcaccgat tcaaccgaag
    13321 cagaagcgat taaacttttc gctaatacct atctggcgat gcgcgtagca tactttaatg
    13381 aactggatag ttatgcagaa agtttaggtc tgaatactcg ccagattatc gaaggcgttt
    13441 gtctcgatcc gcgtattggc aaccattaca acaatccgtc gtttggctat ggtggttatt
    13501 gtctgccgaa agataccaag cagttactgg cgaactacca gactgtgccg aataacctga
    13561 tttcggcaat tgtcgatgca aaccgcacgc gtaaagattt tattgccgat gccattttgt
    13621 cacgcaagcc gcaagtggtg ggtatttatc gtctgattat gaagagcggt tcagataact
    13681 tccgcgcgtc ttccattcaa gggattatga agcgtatcaa ggcgaaaggc gttgaagtga
    13741 tcatctacga accggtgatg aaagaagact cattcttcaa ctctcgcctg gaacgtgatc
    13801 tcgccacctt caaacaacaa gccgatgtca ttatttccaa ccgtatggca gaagagctta
    13861 aggatgtggc agacaaagtc tacacccgcg atctctttgg cagcgactaa catcctgtta
    13921 tcagggcgat tttcgccctg attctcttat gttccctttg taataattca ttatttttat
    13981 catttatcct atagcattca tggcgattat cgctaaacta tggcgactta aaattatttc
    14041 gtccgttagg gtaatgatga gagtagaaaa taataatgtt tctgggcaaa accatgaccc
    14101 ggaacagatt gatttactgg tgcagttgtg gcgtggcaag atgacaatta tcatctctgt
    14161 cattgttgct attgccctgg ctatagggta tctggccgtt gccaaagaaa agtggacttc
    14221 cacagctatc gttactcaac ctgatgtggg acaaatcgct ggttataaca atgccataaa
    14281 tgtgatttat ggctcggcag cgccgaaagt gtctgagatc cagtcgatgt taattggtcg
    14341 tttcagcacg acattttctg cattagcaga aacgctggat aatcaggaag aacctgaaaa
    14401 actgaccatt gagcctaccg ttaaaaatca gtcattacct ttagcggttt cttatgtggg
    14461 tgaaacacct gaaggtgcgc agaaacagct ggcgcagtat atccagcaag ttgacgacca
    14521 ggtaaatgaa gaactggaac aagacctaaa agacaacatc gcgctgcaaa tgaaaaactt
    14581 gcaggattct ctgaagacgc aggaagtggt cgctcaagaa cagaaagatc tgcgtatccg
    14641 tcagattcag gaagcgttgc agtatgcgaa tcaggcgcag gtgacaaagc cgcagattca
    14701 gcagacacag gacgtaaccc aggatactat gttccttttg gggagcgaag cgctggagtc
    14761 gatgattaag cacgaggcga ctcgtccgtt ggtgttctca tcaaactact atcagactcg
    14821 tcaaaacctg ctggatatcg acaatcttga cgttgataaa cttgatattc atgcttaccg
    14881 ctatgtaatg aaaccgacgt tacctattcg tcgcgatagc ccgaaaaagg caattacctt
    14941 gattctggcg gtgctgctgg gcggcatggt tggcgcgggg attgtgttgg ggcgtaacgc
    15001 tctgcgtaat tacaacgcga agtaatcttt tcggttttaa agaaaatggg cagggtggtg
    15061 acaccttgcc cgtttttttg ccggatgcga caacaatatc gcatccgctt accccgcaac
    15121 tcactgatgc cgtttacgca ggttctcaat caccgtcgtt aagtccagat cctgatcctg
    15181 caacagcact agcaggtgat acatcaagtc agatgcctca ttggtcagtt caaagcggtc
    15241 atgtaccgtt gctgccagtg cggtttccac cccttcttcg cccactttct gcgcaatgcg
    15301 tttggt
//
