LOCUS       AB812035               13926 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: H17a.
ACCESSION   AB812035
VERSION     AB812035.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 13926)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..13926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H17a"
                     /db_xref="taxon:562"
                     /note="serogroup:O83"
     gene            <1..897
                     /gene="wcaM"
     CDS             <1..897
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01274.1"
                     /translation="ILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDH
                     VIERINCTNGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVENGK
                     HFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGK
                     YLSIPQNFRVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQH
                     LFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVEI
                     DRINHHIVNVEKINFRLPERRE"
     gene            1072..1965
                     /gene="galF"
     CDS             1072..1965
                     /gene="galF"
                     /note="O083_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01275.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            2301..3743
                     /gene="wzx"
     CDS             2301..3743
                     /gene="wzx"
                     /note="O083_02"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01276.1"
                     /translation="MSTFNNAKWVGLSQFTKVILQIMALIIFSRLLDPLEYGIMAMAT
                     VISNFALIIRDLGTGAAVIQRKVISNDLLSSIFWLNIIMGLLVMVAIIICAPLLSEFF
                     KESKLCNVLLLLSVTFPLASAAIVHQANLEREFQFAKVCSIEIASSLISFLIGIVCAI
                     KNYGVYSLVALTISQAMISTLGMWFASKWRPQFKVNWSEIQEVYSFSGNLTLFNMVNY
                     FSRNSDGIIIGRFFSSAILGAYSLAYRIMLFPVQSLTSVISRSLYPVISRMQDDVNSI
                     NHVYMRTLAFISTLTLPLMMGLWSIRNEFVMEILGAKWISVVGILSWLAPTGYIQSLV
                     STTGTILMACGRANILFYLGVAAAVLQVSAFLIGAQFNITTLSMLYFYSNLINSFIAM
                     YFVMKCINGSLMILIKKLVPSIVSTGVMLIYVKIILKIKEIWLWSDLVTLIVSILGGM
                     TIYTFIYIVFFKQSIRENFPSTLSTKIIKS"
     gene            3740..4975
                     /gene="wzy"
     CDS             3740..4975
                     /gene="wzy"
                     /note="O083_03"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01277.1"
                     /translation="MRNRILTLMQASAIFMFLTKLPMTTVLVVILSLATIFYKLLQNK
                     LYFDKLNNKTIVVIIAMLLYMLCRIMSQYVVGIDLYDVDIDYIVTISLAFILFVSILF
                     HRDAYAILYAIIASSLFTGGWAIFELIFNFNTLSSRFSDPGNIFYMSGNRVPTAFYYN
                     ENDMLYFLVLFLPITFFFFRRRLVSLVYFLMVLTLALYIASKAAVITLLIYVMWYFIF
                     GVKNISDILKRIVLSILFVSIGVLILYLFGKDALDLQIIDKVVYRFSGLVDFINGNGG
                     DSSSLERFSIYLAVASFIYSNISMLFVGFGNFSYYEGIILNHYPLRIADFHNMHFEII
                     TLFGLPFYILLGCVFFYMYKMNKNISFNNTKVFKLMMLTFLTFLAILSSSVLKYPSFY
                     VFIMLLAVLCENKSRVNEY"
     CDS             4965..5870
                     /note="O083_04"
                     /gene="wfeA"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01278.1"
                     /translation="MNIKVSIVIPTFGRPENLLRAISSVMRQTYNNLEIIVVDDNGKG
                     SENQIKTEELLNDEKYKDIIYIVLPNNKGGGIARNYGIEKASGEVITFLDDDDYYLCN
                     KVEVQLRHLIVNNLDISLCDMIIESDGSSRFKHSKRYSNAKGININEFLIDGVAFTPM
                     IMVRKNILLSAGGFLDTPRFQDHTLMLKLFLITDKVGHVESKLFVHCSNMNTRVSNSP
                     KSLKGFLIRHRLENYVMKNQGNIVEQVKFNQCAQVSPFLYKRRGYNKYVSFMIWSLNY
                     NLSVKNVLLTFARVLKISCLAIFKD"
     CDS             5846..6565
                     /note="O083_05"
                     /gene="wfeB"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01279.1"
                     /translation="MLSNIQRLVRLLRLSLSTIDMGDNYNIIPQKKFTLSGVYRIKNA
                     ESSIELAIRSIIDVMDEVIVVDNESSDGTLDILIKLQKKYPNKIKIFHYNKKLCRAGK
                     NYADCVRSNPSGSLAKYYNYAFSKATSEYVMKCDANYIFTLKGKIDIINALNKNPDVL
                     CYPGVEIFGHHHSIEPFVYLRKLNYKYCDGLLWEFLHYERTAKIKKILNPCFVHIKRI
                     NYNKYIHSEHKGVDGLYGSHD"
     CDS             6549..7094
                     /note="O083_06"
                     /gene="werI"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ01280.1"
                     /translation="MVATIKKITKQIVFFINFIFKGKNLVCLSNCREKYNLTREDIDY
                     IANETLHKSHKKINLTLLIREIDLYLLDITINSWAPISKCVNFKNINGVNDNFLKKIT
                     CEYGKNYLGHCEESGCFVPLGVILLPKWYNHFNLNCHVLNGVDIDGRRWQLDLTSGAL
                     NLVDCYNDLDVHFIMMRVNCD"
     CDS             7087..7797
                     /note="O083_07"
                     /gene="wfeD"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01281.1"
                     /translation="MIDDIIKKTPNVIDIMGENCSNSRVITFVNPFSYFSVKDNPRVE
                     EIDYIFIDGILLVKLFNFTNKYNLERYSFDYSSLAGVVFNFCEREKLKVGLIGATSDE
                     IRVATKNIKMIHPQLTVEYTHSGYFLDDQERNNIINTLAENCDVIICGMGTPVQEEFA
                     LDLKALSRNKLIFTCGGFFSQTARKPDFYYSWVKKYNLMWLQRIIMYKHVRKRFFFDY
                     PKFVYKYIKQNLRNSSKR"
     CDS             7808..8629
                     /note="O083_08"
                     /gene="werJ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01282.1"
                     /translation="MLSINLTTTASRLELCSSTLWSLLHQTIIPDEINVWVSKEPYLA
                     DEGINEIPIFITELNSVRNIIKIKFTKNIGPYRKIIPILRESNADDIIVYADDDVIYG
                     CTWLETLYDEFLKNSCSVVVASRVRLMQYNVFGKLKSYLSFPLCTESILLENDFIITG
                     VGGCILSRSIIKDEFITNNEFMSIAPTTDDIWISKIIELSGGFVKCLPVNLKNINEIS
                     HEINALSQDNMVIKYKSNILIRFANKIINKVKCYFCLISSGNDVAIKKVNRYFDN"
     gene            8855..10261
                     /gene="gnd"
     CDS             8855..10261
                     /gene="gnd"
                     /note="O083_09"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01283.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENLGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            10510..11676
                     /gene="ugd"
     CDS             10510..11676
                     /gene="ugd"
                     /note="O083_10"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01284.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVEMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            11766..12800
                     /gene="wzz"
     CDS             11766..12800
                     /gene="wzz"
                     /note="O083_11"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01285.1"
                     /translation="MAIIAKLWRLKIILSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGS
                     AAPKASEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEQDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSSYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(12986..13597)
                     /gene="hisI"
     CDS             complement(12986..13597)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01286.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPKTSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     4174 a   2485 c   2958 g   4309 t
ORIGIN
        1 atcttacgcc agggatttca taaccagatt atcggtgcca acatcaccaa ctgtaagttc
       61 agcgacttac agggggacgc tattgaatgg aacgtggcga ttaacgacag tgatattttg
      121 atatctgacc atgtcatcga gcgcatcaac tgtactaacg gcaaaatcaa ctggggcatc
      181 ggcataggcc ttgcgggaag tacctacgat aataattatc cggaaaacca ggcagtgaaa
      241 aactttgtcg tggcgaatat cacgggatca gattgtcggc aactgatcca tgttgaaaat
      301 ggtaaacatt ttgttattcg taatatcaaa gcccgcaata tcacaccgga tttcagtaag
      361 aaagcaggta ttgataacgc cacagttgct atttatggtt gtgacaactt tgtgattgat
      421 aatattgaaa tgattaatag tgccgggatg ttaatcggct atggggtaat taaaggcaaa
      481 tatctctcga taccgcagaa tttccgagtg aatgatattc aactggataa tactcatctt
      541 gcttataaat tgcgcggcat ccaaatctcc gccgggaatg ctgtctcctt tgtggcgctg
      601 actaacattg agatgaagcg tgcgtcgctg gagttacaca acaagccgca acaccttttt
      661 atgcgaaata tcaatgtgat gcaggaatcc tcagttggac ccgcattgag catgaacttt
      721 gacatgcgca aagacgttcg aggcgttttt atggcgaaaa aagaaacact gctgtctctt
      781 gcaaatgttc atgcggtgaa tgaaaaaggg caaagttccg tcgagatcga caggattaat
      841 caccatattg ttaatgtgga aaagattaac tttagattgc cggaacggag ggagtagatt
      901 tgcgaccatt cctggaaaaa tggagtcata cttaggaaca atgctactgc aatccacaac
      961 gaagcggcgt aacatcacaa gtaattcagt aatcaattca gggtaattga tgctggcgaa
     1021 aaaaatcgaa caagctataa ttcagcaacc attttacagg tggatgaaat aatgacgaat
     1081 ttaaaagcag ttattcctgt agcgggtcta gggatgcata tgttgcctgc cactaaggcg
     1141 attcccaaag agatgctacc gatcgtcgac aagccaatga ttcagtacat tgttgacgag
     1201 attgtggctg cagggatcaa agaaatcctc ctagtaactc acgcgtccaa gaacgcggtc
     1261 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
     1321 caactgctgg cggaagtgca atctatctgc ccgccgggcg tgactattat gaacgtgcgt
     1381 cagggcgaac ctttaggttt aggccactcc attttgtgtg cgcgacctgc cattggtgac
     1441 aacccatttg tcgtggtact gccagacgtt gtgatcgacg acgccagtgc cgacccgcta
     1501 cgctataacc ttgctgccat gattgcgcgc ttcaatgaaa cgggccgcag ccaggtgctg
     1561 gcaaaacgta tgccgggcga tctctctgaa tactccgtca tccagactaa agagccgctg
     1621 gaccgtgagg gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
     1681 acgctggact cagacatcat ggccgtgggc cgttatgtgc tttctgccga tatttggccg
     1741 gaacttgaac gcacgcagcc tggtgcatgg ggacgtattc agctgactga tgctattgcc
     1801 gagctggcga aaaaacaatc cgttgatgca atgctgatga ccggcgacag ttacgactgc
     1861 ggcaaaaaaa tgggctatat gcaggcgttt gtgaagtatg gcctacgcaa cctgaaagaa
     1921 ggggcgaagt tccgtaaagg gattgagaag ttgttaagcg aataatgaaa atctgaccgg
     1981 atgtaacggt taacaagaaa attataacgg cagtgaagat tcgtggcgaa agtaatttgt
     2041 tgcgaatttt cctgccgttg ttttatgctt tttagagaat aacaagtgtg aacaatagtc
     2101 tgaggcttaa acttttccgt gatttccctt gaatttcctt gtttctggag atgaatgata
     2161 agacaattaa catttcagat tccagttaga cttgaggatg aattattcta tacatcgtag
     2221 acatgtacgc agcgctcagg tagctgttaa gcttggggcg gtagcgtgcg taatattaat
     2281 aaaccgtgga gataagataa ttgtctacgt tcaataatgc taaatgggtt gggctatcac
     2341 agtttactaa agttatctta cagataatgg cactaataat ctttagtagg ttattggatc
     2401 cgcttgaata tggtattatg gcaatggcta cagttatatc taacttcgct cttattataa
     2461 gggatttggg aacaggtgca gccgttattc aaaggaaagt tatcagtaat gatttgctat
     2521 cttcaatatt ttggctgaat atcatcatgg ggttattagt aatggtggca ataattattt
     2581 gtgctccatt attatctgaa ttcttcaaag aatcaaaatt atgcaatgtg ttgttattac
     2641 tttcagtaac attccctctg gctagtgcag cgattgtaca ccaggcaaac ctcgaaagag
     2701 aatttcaatt tgcaaaagtt tgttcaattg aaatagcttc ttcattaata tccttcttaa
     2761 ttggaatagt ctgtgctatc aaaaattatg gtgtctatag tttagtcgca ttaacaatca
     2821 gtcaagcaat gatttcaact cttggaatgt ggtttgcttc aaaatggaga ccacagttta
     2881 aggttaattg gtctgaaatt caggaagttt attcttttag tggtaatctg actcttttta
     2941 atatggtgaa ttatttttca agaaatagtg atggaattat tatcggtaga tttttttcga
     3001 gtgccattct aggtgcctat tcattagctt acagaataat gttatttcct gtacaaagct
     3061 taacctcagt tatatcaaga tcattatatc cagtgataag caggatgcaa gatgatgtca
     3121 atagtatcaa tcatgtttac atgagaacgt tagcgttcat ttctacttta actttaccac
     3181 tcatgatggg gttatggtcg ataaggaacg aatttgttat ggaaatttta ggagccaaat
     3241 ggatttcagt tgtgggaata ctttcatggt tggcacctac aggatatatc caatctcttg
     3301 ttagtacaac aggtactatt ttaatggcgt gtggtagagc caatatatta ttttacctcg
     3361 gtgttgcagc tgctgtttta caagtttctg catttcttat tggggcacaa tttaatatta
     3421 caacactctc aatgctttat ttctattcaa acttaattaa tagctttatt gctatgtatt
     3481 ttgtaatgaa atgtataaat ggaagcttga tgatcttaat taaaaaatta gtcccttcga
     3541 ttgtctctac aggtgttatg cttatatatg tgaagattat tttgaaaatt aaagagatat
     3601 ggttatggag tgatttagta acattgattg tttcaattct gggcggtatg actatatata
     3661 cttttattta tatagtcttt ttcaaacaat cgattagaga aaattttcct tcaacattat
     3721 ctactaaaat aataaaatca tgagaaatag aatacttacc ttgatgcaag ccagtgcgat
     3781 ttttatgttt cttacaaaat tgccgatgac tactgtactt gtggttatat tatccttagc
     3841 aacaattttt tataaacttc ttcagaataa actatacttt gataagttaa ataataaaac
     3901 aatagtggta attatcgcaa tgttgctata tatgttatgt cgaattatgt cgcaatacgt
     3961 ggtaggtata gatttatatg atgtggacat tgattatata gtgacaatat cattagcgtt
     4021 tatattgttt gtatctattt tatttcatcg agatgcatat gcaatattgt atgcaattat
     4081 agcatcttca ttatttactg gaggatgggc aatatttgaa ttaatattta attttaatac
     4141 tctatcttca cggtttagcg atcctgggaa tattttttac atgtcaggta atagagtgcc
     4201 aacagcattc tattataatg aaaatgatat gctttatttt ttggttttgt tcttaccaat
     4261 tacctttttt ttctttagga gacgcttggt gtcattagta tattttttaa tggtgctgac
     4321 gcttgctcta tatattgcat ctaaagctgc ggttataaca ttacttattt atgtaatgtg
     4381 gtattttatt tttggtgtaa aaaatataag tgatatatta aaaaggatcg tgctatcaat
     4441 actttttgtc tctattggtg tgctgattct atatttgttt ggaaaagacg cattagacct
     4501 acaaataata gacaaggtcg tatatagatt ttctgggttg gttgatttta ttaatggaaa
     4561 tggcggagac agtagttcat tagagcgatt ttcaatttat ttggccgtcg caagttttat
     4621 atatagtaat attagtatgc tattcgtagg ttttggcaat tttagctatt atgaaggaat
     4681 aatattgaat cactacccgt taagaatagc ggactttcat aacatgcatt ttgaaattat
     4741 cacattgttt ggtcttccat tttatatatt gttaggttgt gtgttttttt atatgtataa
     4801 aatgaataaa aacatttcat ttaataatac taaggtattt aagttgatga tgttaacttt
     4861 ccttaccttt ctcgctatat tatctagttc tgtgctcaaa tatccatctt tttatgtttt
     4921 tattatgctt ttggctgtgt tgtgtgaaaa taagagtcga gtaaatgaat attaaagtat
     4981 caatagttat ccctaccttt ggtagacctg agaatcttct tagggcaatc agcagtgtaa
     5041 tgagacaaac atataataat ttagaaatta tcgttgttga tgataatggt aagggtagcg
     5101 aaaaccagat taaaacggaa gaattgctta atgacgaaaa gtataaagat ataatataca
     5161 tagttttacc taataataaa ggtggtggaa ttgctaggaa ttacggtata gaaaaagcaa
     5221 gcggtgaagt tattacattt ttggatgatg atgattatta cttatgtaat aaagttgaag
     5281 tccagttacg tcatctaata gttaataact tagatatatc tttgtgcgat atgattattg
     5341 aatctgatgg ttcatcacga ttcaaacatt caaaacgtta ttcaaatgct aaaggaataa
     5401 atataaatga gtttttgatt gatggtgtag cttttacacc tatgattatg gtgagaaaaa
     5461 atatactatt aagtgctgga ggatttttag atacaccacg ttttcaagat catacgctga
     5521 tgctaaagtt atttttgatt acggataagg tcggtcatgt tgaaagtaaa ttatttgtac
     5581 attgttccaa tatgaataca agagttagta actctcccaa atcgttgaag gggtttctaa
     5641 tacgacacag attagaaaac tatgtgatga agaaccaagg gaatatagta gagcaagtta
     5701 aattcaatca atgcgcacaa gtatctcctt tcctttataa aagaaggggt tacaataaat
     5761 atgtttcctt catgatctgg tctttaaatt ataatcttag tgtaaaaaat gtattattaa
     5821 catttgcgag ggttttaaaa atatcatgct tagcaatatt caaagattag taagattact
     5881 aagattatcg ctatcaacaa ttgacatggg tgataattat aatattatac cgcaaaagaa
     5941 attcacatta tctggtgttt ataggataaa gaatgcagag agttcaatag agcttgccat
     6001 tcgttcaatt atagatgtaa tggatgaagt gattgttgta gataatgaat cttcagatgg
     6061 tacattagat attttaatta aactgcaaaa aaaatatcct aataaaataa agattttcca
     6121 ttataacaaa aaactgtgta gagcaggtaa aaattatgct gattgtgtga gaagtaatcc
     6181 atcagggagc ttagccaaat attataacta tgctttttcc aaagcaacta gcgaatatgt
     6241 aatgaagtgt gatgccaatt atattttcac gttgaaaggt aaaatagata ttatcaatgc
     6301 attaaataag aatcctgatg tattatgcta tcctggtgtt gaaatatttg ggcaccatca
     6361 ctcaattgag ccatttgtat atttaaggaa attaaattat aaatactgtg atggtttgtt
     6421 atgggagttt ttacattatg aacgaactgc gaaaataaag aaaatattaa atccgtgctt
     6481 tgtgcatata aaaagaatca attacaataa gtatatacat agcgaacata aaggagttga
     6541 cggtttatat ggtagccacg attaaaaaaa taactaagca aattgttttt ttcattaact
     6601 ttatatttaa aggtaaaaac ctagtatgtt tatcaaattg tcgcgaaaag tataatttaa
     6661 ctagagaaga tatagattat atagccaatg aaacattgca taaatctcat aaaaagatta
     6721 atttaacctt actaattaga gaaatagatc tttacctttt agatataact ataaactcat
     6781 gggcaccgat ttcaaaatgt gttaacttta aaaatataaa cggagttaat gataatttct
     6841 taaaaaaaat tacctgtgag tatgggaaaa attatcttgg tcattgtgag gaatctggat
     6901 gttttgtgcc cttaggtgtc attcttttac ctaaatggta taaccatttt aatttaaatt
     6961 gccatgtgtt aaatggagtt gatattgatg gtagacgatg gcaactggac cttacctctg
     7021 gagcgcttaa tcttgttgat tgctataacg atcttgatgt ccattttatt atgatgaggg
     7081 tgaattgtga ttgatgatat aatcaaaaag acaccaaatg taatcgatat tatgggcgaa
     7141 aactgctcta attctcgagt aattacattt gtaaacccat tttcatattt ttcagtgaag
     7201 gataacccta gagtcgagga aatagattat attttcattg acggtatatt gcttgtcaaa
     7261 ctgtttaatt ttacgaacaa atataatctt gaaagatata gtttcgatta ctcctcattg
     7321 gcaggtgtag tgtttaattt ttgtgaaagg gaaaaactaa aagttggact tattggtgct
     7381 accagtgatg aaattagagt tgcaacaaaa aatattaaaa tgattcatcc acaactgact
     7441 gtggaatata ctcactctgg atatttttta gatgatcaag aacgtaataa tattattaat
     7501 acgttagcag aaaactgcga tgtcatcatt tgcggtatgg gaactcccgt acaagaagaa
     7561 tttgcattag acttgaaagc attatctcga aataagttaa ttttcacttg tggaggtttt
     7621 ttttcccaaa cggcaagaaa acctgatttt tattattcgt gggtcaaaaa atataacttg
     7681 atgtggcttc aaagaattat aatgtacaaa catgttcgaa aacgcttttt ttttgattat
     7741 ccaaaatttg tttataaata tataaagcaa aatttacgca attcttctaa gaggtgataa
     7801 tttcgaaatg ttatcaatta atttaacaac tacagcgtca cgtttagaac tatgttcatc
     7861 aactttatgg tcattattac atcaaacaat tatacccgat gaaataaatg tatgggtatc
     7921 gaaagagccc tacttggctg atgagggcat aaatgagata cctatattta taacagaatt
     7981 gaattcagtt agaaacatta tcaaaattaa atttacaaaa aatataggtc cctacagaaa
     8041 aataattcca atacttagag aaagtaacgc agacgacatc attgtatatg ccgacgatga
     8101 tgtcatttat ggatgcactt ggcttgaaac attatatgat gagtttttga aaaactcatg
     8161 ttctgtagtt gtagcctcta gggttaggct tatgcagtat aatgtatttg gtaaattgaa
     8221 aagttattta tcgtttccat tatgcactga aagtattttg cttgaaaatg attttattat
     8281 taccggggta ggtggctgta ttttatcccg ctcgatcatt aaagatgagt ttattacaaa
     8341 taatgaattt atgtccattg cacctacaac tgatgatata tggattagta aaatcataga
     8401 actttctggg ggatttgtaa aatgcttacc tgttaactta aaaaacataa atgaaatttc
     8461 acatgaaatc aatgcattaa gccaagacaa catggtgata aaatataaaa gtaatatttt
     8521 aattcgtttc gcaaataaaa ttattaataa ggttaagtgc tatttttgtt tgatttcatc
     8581 aggaaatgat gttgcaataa aaaaagtaaa tcgttatttt gataattaat tttaatgggg
     8641 tgttaattga attgacattt cagttatatt ccttcacgac aactaagggg tttatgaatt
     8701 taatgctctg taattactta aataataagt ctcagcgtaa cgcttaaaag ttttaattga
     8761 atttaaaatg gatctgtttt taagttaagt attagattat catttactat ccataaatca
     8821 tttcacatgt tcacccccct gacaggagta aataatgtca aagcaacaga tcggcgtcgt
     8881 cggtatggca gtgatggggc gcaaccttgc gctcaatatc gaaagccgtg gttataccgt
     8941 ctctattttc aaccgttccc gtgaaaagac cgaagaagtg attgccgaaa atctaggcaa
     9001 gaaactggtt ccttactata cggtgaaaga gtttgttgaa tctctggaaa cgcctcgtcg
     9061 catcctgtta atggtgaaag caggtgcagg cacggatgct gctattgatt ccctcaagcc
     9121 atacctcgat aaaggtgaca tcatcattga tggtggtaat accttcttcc aggacaccat
     9181 tcgtcgtaac cgtgagcttt ctgccgaagg ctttaacttc attggtaccg gtgtttccgg
     9241 aggtgaagaa ggtgcgctga aaggtccttc cattatgcct ggtgggcaga aagaagccta
     9301 tgaacttgtt gcgccgatcc tgaccaaaat cgccgcagtg gctgaagacg gcgagccatg
     9361 cgttacctat attggtgccg atggtgcagg tcactatgtg aagatggttc acaacggtat
     9421 tgaatacggt gatatgcagc tgattgctga agcctattct ctgcttaaag gtggcctgaa
     9481 tctcaccaac gaagaactgg cgcagacctt taccgagtgg aataacggtg aactgagcag
     9541 ttacctgatc gacatcacca aagatatctt caccaaaaaa gatgaagacg gtaattacct
     9601 ggttgatgtg atcctggatg aagcggctaa caaaggtacc ggtaaatgga ccagccagag
     9661 cgcgctggat ctcggagaac cgctgtcgct gattaccgag tctgtgtttg cacgttatat
     9721 ctcttctctg aaagagcagc gtgttgccgc atctaaagtt ctctctggcc cgcaagcgca
     9781 gccagctggc gacaaaggtg agttcatcga aaaagttcgt cgtgcgctgt atctgggcaa
     9841 aatcgtttct tacgctcagg gcttctctca gctacgtgcg gcgtctgaag agtacaactg
     9901 ggatctgaat tacggcgaaa tcgcgaagat tttccgtgct ggctgcatca tccgtgcgca
     9961 gttcctgcag aaaatcaccg atgcatatgc cgaaaatccg cagatcgcta acctgctgct
    10021 ggctccgtac ttcaagcaaa ttgccgatga ctatcagcag gcgctgcgcg atgtcgtcgc
    10081 ttatgcagta cagaacggta tcccggttcc gaccttcgcc gctgcggttg cctattatga
    10141 cagctaccgt gccgctgttc tgcctgcgaa cctgatccag gcacagcgtg actatttcgg
    10201 cgcgcatact tataagcgca ttgataaaga aggtgtgttc cataccgaat ggctggatta
    10261 atctgattta aatcaattaa tcaatgcaag gcccggagaa accctccggg cttttttatt
    10321 atacaaagcg gcaggttatg gccttttttt ataatttatg gttaaaaaag cgatataata
    10381 cagcgccgca cagcaggatc gctgccttga cagttcatct acatcagcgt taaaaatccc
    10441 gcagtagatg aagttgtggt gatggattaa tgaccactct aaatgtttaa ccggaagaag
    10501 tcagagctaa tgaaaataac aatttcagga acaggttatg ttggtctttc aaatggtatt
    10561 ctgattgcgc aaaaccacga agtggttgca ctggatatcg ttcaggccaa agtggagatg
    10621 cttaacaaga agcagtcacc gattgttgat aaggagattg aagagtatct ggcgactaaa
    10681 gatctcaatt tccgcgctac gacagataag tatgacgcgt ataaaaatgc cgattacgtt
    10741 attattgcca ctcctaccga ttatgacccg aaaacaaatt attttaatac ctcaagtgtc
    10801 gaagcggtca tccgtgatgt gacagaaatt aatcccaacg cggtaatggt gattaaatct
    10861 acaattccgg taggtttcac caaatcaatt aaagaacgtc tgggtatcga taaccttatt
    10921 ttctctccgg aatttctccg tgaaggtaaa gctctttacg ataatctcca tccttcacgt
    10981 attgtcattg gcgagcgttc agaacgcgca gaacgttttg ctgcgttgtt acaggaaggc
    11041 gcgattaagc aaaatatccc gaccctgttt accgactcca ctgaagcaga agcgattaaa
    11101 ctgtttgcta atacctatct ggcgatgcgc gtagcatact ttaatgaact ggatagctat
    11161 gcagaaagtt taggtctgaa tactcgtcag attatcgaag gtgtttgtct cgatccgcgt
    11221 attggcaacc attacaacaa tccgtcgttt ggctatggtg gttattgtct accgaaagat
    11281 accaagcagt tactggcgaa ctaccagtct gtgccgaata acctgatctc ggcaattgtc
    11341 gatgcaaacc gcacgcgtaa agactttatt gccgatgcca ttttgtcacg caagccgcaa
    11401 gtggtgggta tttatcgtct gattatgaag agcggttcag ataacttccg cgcgtcttcc
    11461 attcagggga ttatgaaacg tattaaggcg aaaggcgttg aagtgatcat ctacgagccg
    11521 gtgatgaaag aagactcatt cttcaactct cgtctggaac gtgatctcgc caccttcaaa
    11581 caacaagccg acgtcattat ttccaaccgt atggcagaag agcttaagga tgtggcagat
    11641 aaagtctaca cccgcgatct ctttggcagc gactaacatc ctgttatcag ggcgattttc
    11701 gccctgattg tcttatgttc cctttgtaat aattcattat ttttatcatt tatcctatag
    11761 cattcatggc gattatcgct aaactatggc gacttaaaat tattttgtcc gttagggtaa
    11821 tgatgagagt agaaaataat aatgtttctg ggcaaaacca tgacccggaa cagattgatt
    11881 tgattgattt actggtccaa ctatggcgtg gcaagatgac tatcatcatt tctgtcattg
    11941 tggctattgc cctggctata gggtatctgg ccgttgccaa agaaaagtgg acttcaacag
    12001 ctatcgttac tcaacctgat gtggggcaaa tcgctggtta taacaatgcc ataaatgtga
    12061 tttatggttc ggcagcgccg aaagcgtctg agatccagtc gatgttaatt ggtcgtttca
    12121 gcacgacatt ttctgcatta gcagaaacgc tggataatca ggaagaacct gaaaaactga
    12181 ctattgagcc taccgttaaa aatcagtcat tacctttagc ggtttcttat gtcggtgaaa
    12241 cacctgaagg tgcgcagaaa cagctggcga agtatatcca gcaagttgac gaccaggtaa
    12301 atgaagaact ggaacaagac ctgaaagaca acatcgcgct gcgaatgaaa aacttgcagg
    12361 attctctgaa gacgcaggaa gtggtcgctc aagaacagaa agatctgcgt atccgtcaga
    12421 ttcaggaagc gttgcagtat gcgaatcagg cgcaggtgac aaagccgcag attcaacaga
    12481 cgcaggacgt aacccaggat actatgttcc ttttggggag cgaagcgctg gagtcgatga
    12541 ttaagcacga ggcgacccgt ccgttggtgt tctcatcaag ctactatcag actcgtcaaa
    12601 acctgctgga tatcgacaat cttgacgttg ataaacttga tattcatgct taccgctatg
    12661 taatgaaacc gacgttacct attcgtcgcg atagcccgaa aaaggcaatt accttgattc
    12721 tggcggtgct gctgggcggc atggttggcg cggggattgt gcttgggcgt aacgcgctgc
    12781 gtaattacaa cgcgaaataa tattattgtg catttaagag aaacgggcag ggtggtgaca
    12841 ccatgcccgt tttttttgcc ggatgcgatg ctggcgcatc ttatccggcc tacgtgtgtt
    12901 gagataatgt gtaggcacga taagcttgcg catcgggcaa tggctccggg tgtgacaaca
    12961 acatcacacc tgctcagcac gccactcact gatgccgttt acgcaggttc tcaattaccg
    13021 ttgttaaatc cagatcctga tcctgcaaca gcaccagcag gtgatacatc aaatcagacg
    13081 cctcgttggt cagctcaaag cggtcatgta ccgttgctgc tagtgcggtt tccacgcctt
    13141 cttcgcccac tttctgtgca atgcgtttgg tgccgctggc atacagtttg gctgtgtaag
    13201 aggttttcgg gtcggcagat ttacgttcgg cgagcagttg ttccagttga tacaggaaca
    13261 gccactggtg agcggtatcg ccgaagcagc tgctggtgcc tttgtggcag gttggtccga
    13321 taggattcgc cagtaccagt agcgtgtcgt tgtcgcagtc cggcgcaata tttactacgt
    13381 tgaggaaatt gcccgacgtt tcgcctttgg tccacagccg ttgtttagtg cgcgagaaga
    13441 aggtgacttt gccgctttcg atggttttgt ctaaagcttc cgggttcata tagcccagca
    13501 ttagcacttc gccggatacc gcgtgttgca caatcaccgg cataagtccg tcggtttttt
    13561 cccagtccag ttcgcgacgt tgttgttctg ttaacatatc ctgatctcca cgccctgtgt
    13621 tgccaggtac gcttttaatt caccaatatt tattatttgt ttgtggaata cggaagctgc
    13681 cagcgcgccg tcaacgtcgg catcgcggaa ggcttcgaga aagtgttcca tggtgcctgc
    13741 gccaccggag gcaatcagcg ggacgtggca aacttcacgc acttttttca gctgttcgag
    13801 gtcgtaaccg ttacgcacgc cgtcctgatt catcatgttg aggacgattt ctccggcacc
    13861 gcgtttttgc acttcctgca cccaatcgag tgtttcccac tgagtgacgc gggtgcggct
    13921 ttcatc
//
