LOCUS       AY670704               14156 bp    DNA     linear   BCT 27-MAR-2005
DEFINITION  Escherichia coli O86 O-antigen gene cluster, complete sequence.
ACCESSION   AY670704
VERSION     AY670704.1  GI:56384971
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14156)
  AUTHORS   Feng,L., Han,W., Wang,Q., Bastin,D.A. and Wang,L.
  TITLE     Characterization of Escherichia coli O86 O-antigen gene cluster and
            identification of O86-specific genes
  JOURNAL   Vet. Microbiol. 106 (3-4), 241-248 (2005)
   PUBMED   15778030
REFERENCE   2  (bases 1 to 14156)
  AUTHORS   Feng,L., Han,W., Wang,Q., Bastin,D. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2004) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..14156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O86"
     gene            1075..2094
                     /gene="gne"
     CDS             1075..2094
                     /gene="gne"
                     /note="UDP-N-acetylglucosamine 4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="AAV85952.1"
                     /db_xref="GI:56384972"
                     /translation="MVIFVTGGAGYIGSHTILELLNNGHDVVSIDNFVNSSIESLKRV
                     EQITNKKIISYQGDIRDKNLLDEIFSRHHIDAVIHFASLKSVGESKLKPLEYYSNNVG
                     GTLVLLECMKRYNINKMIFSSSATVYGSNSIPPHTEDRRIGETTNPYGTSKFIIEIIL
                     SDYCDSDNNKSVIALRYFNPIGAHKSGMIGENPNGIPNNLVPYISKVAQNQLPVLNIY
                     GNDYPTKDGTGVRDYIHVCDLAKGHVKALEYMFLNDVNYEAFNLGTGQGYSVLEIVKM
                     FEIVTKKSIPVAICNRREGDVAESWASADLAHKKLSWKAEKNLKEMIEDVWRWQTNNP
                     NGYKK"
     gene            2113..3129
                     /gene="wcmA"
     CDS             2113..3129
                     /gene="wbwH"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcmA"
                     /protein_id="AAV85953.1"
                     /db_xref="GI:56384973"
                     /translation="MKNVGFIVTKSEIGGAQTWVNEISNLIKEECNIFLITSEEGWLT
                     HKDVFAGVFVIPGIKKYFDFLTLFKLRKILKENNISTLIASSANAGVYARLVRLLVDF
                     KCIYVSHGWSCLYNGGRLKSIFCIVEKYLSLLTDVIWCVSKSDEKKAIENIGIKEPKI
                     ITVSNSVPQMPRCNNKQLQYKVLFVGRLTHPKRPELLANVISKKPQYSLHIVGGGERL
                     ESLKKQFSECENIHFLGEVNNFYNYHEYDLFSLISDSEGLPMSGLEAHTAAIPLLLSD
                     VGGCFELIEGNGLLVENTEDDIGYKLDKIFDDYENYREQAIRASGKFVIENYASAYKS
                     IILG"
     gene            3156..4277
                     /gene="gmd"
     CDS             3156..4277
                     /gene="gmd"
                     /note="GDP-D-mannose dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmd"
                     /protein_id="AAV85954.1"
                     /db_xref="GI:56384974"
                     /translation="MAKVALITGITGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLTDTSNLTRILCEVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAERVGIKLRF
                     EGTGVDEKGIVVSVNGEDAPAVKPGDVIVKVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            4280..5245
                     /gene="fcl"
     CDS             4280..5245
                     /gene="fcl"
                     /note="GDP-L-fucose synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="Fcl"
                     /protein_id="AAV85955.1"
                     /db_xref="GI:56384975"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            5245..5748
                     /gene="gmm"
     CDS             5245..5748
                     /gene="gmm"
                     /note="GDP-mannose mannosyl hydrolase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmm"
                     /protein_id="AAV85956.1"
                     /db_xref="GI:56384976"
                     /translation="MMFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQG
                     YWFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTH
                     YVVLGFRFRVAEEELLLPDEQHVDYRWLTPDALLASNNVHANSRAYFNHDPSAVFGLD
                     KKDVKNV"
     gene            5741..7189
                     /gene="manC"
     CDS             5741..7189
                     /gene="manC"
                     /note="GDP-mannose pyrophosphorylase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="AAV85957.1"
                     /db_xref="GI:56384977"
                     /translation="MSDVPLIAVVMAGGTGSRLWPLSREHYPKQFLQLSGENTLLQST
                     LLRLSPLSCETPLVITNEQHRFVVAEQLRQINQLSDNIILEPCGRNTAPAIALSAFTA
                     LKRNEQQDPILLVLAADHVINKTDVFCNAIKNSISIVEQGNILTFGIIPDYAETGYGY
                     IEKGSIVKESQRGVGNTFYHVEQFVEKPNRSRAEEYISSGKYLWNSGMFMFKASVYLE
                     ELKKYRPDIYDICEKTISSSYHDLDFIRLSKDVFQNCPSESIDFAVMEKTKRCIVYPV
                     DIGWNDVGSWQSLWDVSDKTPTGDVCKGDILTYNTKNNYIHSESALVAAVGVEDIVIV
                     QTKDAILVSKKSEVQDVKKIVQMLKMQERSEYLSHREEFRPWGKFDAIEQGDRYKVKK
                     IVVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLNNKTFLVTANESVYIPLGATYSLENP
                     GIIPLNLIEVSSGDYLGEDDIVRQKERYKIDD"
     gene            7193..8581
                     /gene="manB"
     CDS             7193..8581
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="AAV85958.1"
                     /db_xref="GI:56384978"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLIWNTVDVVSAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSTLEDPLCAIAR
                     VESYYANKAIEIDRTDGISMTFNGWRFNLRSSNTEPVVRLNVESRGDVAATKKYTGFI
                     LDLLNYKKSISS"
     gene            8601..9803
                     /gene="wzx"
     CDS             8601..9803
                     /gene="wzx"
                     /note="flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="AAV85959.1"
                     /db_xref="GI:56384979"
                     /translation="MIKLLFSSSLLKMGVVLLNLLIPAIIIRFYSEYDFGIYSYLLTY
                     AIVISVLQNGITASFRNLISNLEKNDLLDYLLYSVRKITYPFLLIIFCLLLATLYVVG
                     FDTLYGKICFFIAVSLINIYYPVVASYYDATGKTLNFVLLEIIFLLVFIAGVGILIYY
                     KVSIYIVCILTANYRGVYAILLIMKKCVIFKQIRLKKKGGYKIFCSDDMIFIIIQLIN
                     VSNTLLFMNVFAKFYGVVAFGVFSVYYRFVAFPQQVISFSSSLIWINFRQVHVNNRVA
                     AMQLLKRLFFIFTLLLIIWGGCVHFFIDKVVYLYSSKPLEYPGVLCFLNIMVCLMLLK
                     DFSSIILNALTLYKEQMIMNALLCLLNVIFFFFYNETNFDTIYLIFVSLLTLMFVFVN
                     LSLIKSRL"
     gene            9806..10510
                     /gene="wcmB"
     CDS             9806..10510
                     /gene="wbwI"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcmB"
                     /protein_id="AAV85960.1"
                     /db_xref="GI:56384980"
                     /translation="MVINIFYICTGEYKRFFDKFYLSCEDKFIPEFEKKYYVFTDSDR
                     IYFSKYLNVEVINVEKNCWPLNTLLRFSYFLKVIDKLQTNSYTFFFNANAVIVKEIPF
                     STFMESDLIGVIHPGYKNRISILYPWERRKNATCYLGYLKKGIYYQGCFNGGKTASFK
                     RLIQICNMMTMADLKKNLIAKVHDESYLNYYYYYNKPLLLSELYSWPEKYGENKDAKI
                     IMRDKERESWYGNIKK"
     gene            10491..11831
                     /gene="wzy"
     CDS             10491..11831
                     /gene="wzy"
                     /note="polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="AAV85961.1"
                     /db_xref="GI:56384981"
                     /translation="MVISRSNYRNICSYTFFMVNLFILILSVINEGFCEIAYVIISVS
                     SVLFCVIIICLERQGGFLNPMTFCIISVFFFILIRPVFFSQNITENLNEVITAGLEID
                     EIYVFYSLAVVNIPLAFTVLLYSVQKGTVSKLVGQLPDLFFYNKQLSMILLWGGLFSA
                     IFLIKSYKKFIILGQVSVFEADAYGLYDELFWFTLSKYCYILSLLFSKNKNFILYSLL
                     IFITSIGYILVGLRGYTIAYGFLLLFFLDIRYRLKIKWLLLVAILVTTISSLFLNYRI
                     GIEVNSGLLGIIFNPLLQQGASFETVYGALKYNEKILSCISYYDYFFTNKDIGSCIDI
                     ARGVYFKEGGSFASSFYSELIYLGWIIGSVALLLFAFSLAFVQSCYEKIIKNSMNNKL
                     AYTYRLIIFLALPNLIYFARSSLFDFITKVLFIALFIGGLSIVRHIALNIKKCH"
     gene            11843..12586
                     /gene="wcmC"
     CDS             11843..12586
                     /gene="wfbH"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbH"
                     /protein_id="AAV85962.1"
                     /db_xref="GI:56384982"
                     /translation="MISVIMAVHRYDKYVDISIDSILNQTYSDFELIIIANGGDCFEI
                     AKQLKHYTELDNRVKIYTLEIGQLSFALNYAVTKCKYSIIARMDSDDVSLPLRLEKQY
                     MYMLQNDLEMVGTGIRLINENGEFIKELKYPNHNKINKILPFKNCFAHPTLMFKKDVI
                     LKQRGYCGGFNSEDYDLWLRILNECPNIRWDNLSECLLNYRIHNKSTQKSALAYYECA
                     SYSLREFLKKRTITNFLSCLYHFCKALIK"
     gene            12598..13506
                     /gene="wcmD"
     CDS             12598..13506
                     /gene="wbwK"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbwK"
                     /protein_id="AAV85963.1"
                     /db_xref="GI:56384983"
                     /translation="MYSCLSGGLGNQMFQYAAAYILQRKLKQRSLVLDDSYFLDCSNR
                     DTRRRFELNQFNICYDRLTTSKEKKEISIIRHVNRYRLPLFVTNSIFGVLLKKNYLPE
                     AKFYEFLNNCKLQVKNGYCLFSYFQDATLIDSHRDMILPLFQINEDLLNLCNDLHIYK
                     KVICENANTTSLHIRRGDYITNPHASKFHGVLPMDYYEKAIRYIEDVQGEQVIIVFSD
                     DVKWAENTFANQPNYYVVNNSECEYSAIDMFLMSKCKNNIIANSTYSWWGAWLNTFED
                     KIVVSPRKWFAGNNKSKLTMDSWINL"
BASE COUNT     4218 a   2509 c   3063 g   4366 t
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc atgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctcttc ttgaacaacg tgtgaagcgt
      121 caactgttgg cggaagtaca gtcgatctgc ccgccgggcg taaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attttatgtg cacgacccgc cattggtgac
      241 aacccatttg tcgtggtact gccagacgtt gtgatcgacg acgccagcgc tgacccgctg
      301 cgttacaacc ttgctgccat gattgcgcgc ttcaacgaaa cgggccgtac ccaggtgctg
      361 gcaaaacgta tgccgggcga cctctcggaa tactccgtta ttcagaccaa agagccgctg
      421 gatcgcgaag gcaaagtcag ccgcattgtt gaattcatcg aaaagccgga tcagccgcag
      481 acgctggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcagcc tggtgcatgg gggcgtattc aactgactga tgccattgct
      601 gaactggcga aaaaacagtc cgttgatgcc atgctgatga caggtgacag ctacgactgc
      661 ggtaaaaaaa tggggtatat gcaggcattt gtgaagtatg gactacgcaa cctcaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ctgttaagcg aataaaaatt aatcatccat
      781 atatggtgga ttgaataaaa atagaacggc agtgaacatt caaaagcaga gatgcttaca
      841 ttataagaat gttcctgtct tttatttaaa gtatttaata acaataagtt accgcaagta
      901 tcgctcgggc atagattttc cttgtatcta acttcgtttg gttagacaat tggaaaatag
      961 tgaaaagtat taaatgctta gtacctatga ttcattttca gtgagttggt aactgttaag
     1021 ccaagggcgg tagcgtgtgt ttttctctca atcctcatgg taaaggaggt ttatatggtg
     1081 attttcgtaa caggcggtgc aggatatatt ggatcccata ccatacttga gttacttaat
     1141 aatggtcatg atgtcgtttc gatagataat tttgtcaatt cctctataga atcattaaaa
     1201 agagtagagc aaataactaa taagaaaatt atttcttatc aaggtgatat ccgtgataaa
     1261 aatctacttg atgagatttt ttcaagacac catatcgatg ctgtaattca ctttgcatcg
     1321 ttaaaatctg taggtgagtc taagttaaag cccttagagt attattctaa taatgttggt
     1381 ggaactttag tattacttga atgcatgaag agatataaca ttaataaaat gatatttagc
     1441 tcttctgcta ctgtttatgg gagtaacagt atccctcccc atacggaaga tagacgaatt
     1501 ggtgaaacta caaacccata tgggacatcg aaatttataa tagaaataat tttgagtgat
     1561 tattgtgata gtgataataa taaatcagta attgcactgc gttactttaa tccaatcgga
     1621 gcacataagt ccgggatgat tggtgaaaat cctaacggga tccctaataa tctggttcct
     1681 tatatatcta aagttgcaca aaatcaactt cctgtattaa atatttatgg caacgattat
     1741 ccaactaaag atggtacagg agtaagagac tatatacatg tctgtgattt ggctaaaggg
     1801 catgttaaag cattagaata tatgttttta aatgatgtca attatgaagc ttttaattta
     1861 ggtactggtc aaggttattc tgttttagag attgtaaaaa tgtttgagat agtcactaaa
     1921 aagagtatac ctgttgctat ttgtaataga cgtgaggggg atgttgcgga gtcatgggcg
     1981 tctgctgatt tggcacataa aaagctttcc tggaaagcgg aaaaaaattt gaaagaaatg
     2041 atcgaagatg tatggcgttg gcaaacaaac aatccaaatg gatataaaaa ataatttttg
     2101 gaattttgat atatgaaaaa tgttggtttt attgttacaa aatcagaaat tggtggtgca
     2161 caaacatggg taaatgaaat atctaacctt attaaagagg aatgtaatat atttcttatt
     2221 acatctgaag aaggatggct cacacataaa gatgtctttg ccggagtttt tgtcatacca
     2281 ggtattaaaa aatattttga cttccttaca ttgtttaaat tgagaaaaat tttaaaagaa
     2341 aataacattt caacgttaat agcaagttct gctaatgccg gagtttatgc caggttagtt
     2401 cgattactag tcgactttaa atgtatttat gtttcgcatg gatggtcttg tttatataat
     2461 ggtggtcgcc taaaatcaat tttttgcatt gttgaaaaat acctttcttt attaactgat
     2521 gttatatggt gtgtttccaa aagtgatgaa aaaaaggcaa ttgagaatat tggtataaaa
     2581 gaaccaaaga taatcacagt atcgaattca gtgcctcaga tgccgagatg taataataaa
     2641 caactccagt ataaggttct gtttgttggt aggttaacac accctaagcg ccccgaattg
     2701 ttagcgaatg taatatcgaa aaagccccag tatagcctcc atatcgtagg agggggggaa
     2761 aggttagaat cattgaagaa acaattcagt gaatgtgaaa atattcattt tttgggtgag
     2821 gtcaataatt tttataacta tcatgagtat gatttatttt cactgatatc cgatagtgaa
     2881 ggtttgccta tgtcaggcct tgaggctcac acagctgcaa taccactcct gttaagtgat
     2941 gtgggcggat gttttgaatt aattgagggt aatgggttac ttgtggaaaa tactgaagac
     3001 gacattggat ataaattgga taaaatattc gatgactatg aaaattatcg ggaacaggca
     3061 attcgtgcct ccgggaaatt tgttatcgag aactatgctt cagcatataa aagcattatt
     3121 ttaggttgag tatttttaag aacaggaaat gtaaaatggc taaagtcgct ctcattactg
     3181 gcataaccgg acaagacggt tcttacctgg cagagtttct gctggaaaaa ggttacgagg
     3241 tgcatggtat caagcgtcgt gcatcgtcat tcaacaccga gcgcgtggat catatctatc
     3301 aggatccgca cacctgcaac ccgaaattcc atctgcatta tggcgatctg acagatacct
     3361 ccaacctgac gcgcattttg tgtgaagtgc agccggatga agtgtacaac ctgggcgcaa
     3421 tgagtcacgt tgcggtttct tttgagtcac cggaatatac cgctgacgtc gatgcgatgg
     3481 gtacgctgcg cctgctggag gcgatccgct tcctcggtct ggaaaagaaa acccgttttt
     3541 atcaggcttc cacttctgaa ctgtatggtc tggtgcagga aattccccag aaagagacca
     3601 caccgttcta cccgcgatct ccgtatgcag tcgccaaact gtacgcctac tggatcaccg
     3661 ttaactaccg cgaatcctac ggcatgtacg cctgcaacgg tattctcttt aaccatgaat
     3721 ccccgcgccg cggcgaaact ttcgttaccc gcaaaatcac ccgcgcaatc gccaacatcg
     3781 cccaggggct ggaatcgtgc ctgtacctcg gcaatatgga ttccctgcgt gactggggcc
     3841 atgccaaaga ctacgtaaaa atgcagtgga tgatgctgca acaggaacag ccggaagatt
     3901 tcgtcattgc taccggcgtt cagtactccg tgcgtcagtt cgtggaaatg gcggcggagc
     3961 gagtcggtat caaactgcgt tttgaaggca caggtgtaga tgagaagggg atagtggttt
     4021 ctgttaatgg tgaggatgct ccagcagtta aacctggaga tgtaatcgtt aaggttgatc
     4081 cgcgttactt ccgtccggct gaagttgaaa cgctgctcgg tgacccgacc aaagcgcacg
     4141 aaaaactggg ctggaaacca gaaatcaccc tcagagagat ggtgtctgaa atggtggcta
     4201 atgacctcga agcggcgaaa aaacactctc tgctgaaatc tcacggctac gacgtggcga
     4261 tcgcgctgga gtcataagca tgagtaaaca acgcattttt atcgctggcc atcgcgggat
     4321 ggtcggttct gccatcaggc ggcagctcga acagcgcggt gatgtggaac tggtattacg
     4381 cacccgcgac gagctgaacc tgctggacag ccgcgccgtg catgatttct ttgccagcga
     4441 acgtattgac caggtctatc tggcggcggc gaaagtgggc ggcattgttg ccaacaacac
     4501 gtatccggcg gatttcatct atcagaacat gatgattgag agcaacatca ttcacgccgc
     4561 gcatcagaac gacgtgaaca aactgctgtt tctcggatcg tcctgtatct acccgaaact
     4621 ggcaaaacag ccaatggcag aaagtgagtt attgcagggc acgctggagc cgaccaacga
     4681 accttatgcc attgccaaaa tcgccgggat caaactgtgc gaatcttaca accgtcagta
     4741 cggacgcgat taccgctcgg tcatgccgac caacctgtac gggccgcacg acaacttcca
     4801 tccgagtaac tcgcatgtga tcccagcatt gctgcgtcgc ttccacgagg cgacggcaca
     4861 gaatgcgccg gacgtggtgg tgtggggcag cggtacgccg atgcgtgaat ttctgcacgt
     4921 cgatgatatg gcggcggcga gcattcatgt gatggagctg gcgcacgaag tctggctgga
     4981 gaacacccag ccgatgttgt cgcacattaa cgtcggcacg ggcgttgact gcactatccg
     5041 cgagctggcg caaaccatcg ccaaagtggt gggttacaaa ggtcgggtgg tttttgatgc
     5101 cagcaaaccg gatggcacac cgcgcaaact gctggatgtg acgcgcctgc atcagcttgg
     5161 ctggtatcac gaaatctcac tggaagcggg gcttgccagc acttaccagt ggttccttga
     5221 gaatcaagac cgctttcggg ggtaatgatg tttttacgtc aggaagactt tgccacggta
     5281 gtgcgctcca ctccgcttgt ctctctcgac tttattgttg agaacagtcg cggcgagttt
     5341 ctgcttggca aaagaaccaa ccgcccggcg cagggttact ggtttgtgcc gggagggcgc
     5401 gtgcagaaag acgaaacgct ggaagccgca tttgagcggc tgacgatggc ggaactgggt
     5461 ctgcgtctac cgataacagc aggccagttt tacggtgtct ggcagcactt ttatgacgat
     5521 aacttctccg gcacggattt caccacgcac tatgtggtgc tcggttttcg cttcagagtc
     5581 gcggaagaag agctgttact gccggatgag cagcatgtcg attaccgctg gctgacgccg
     5641 gacgcgctgc tcgccagtaa caatgttcac gctaacagcc gagcttattt taatcatgat
     5701 ccaagtgctg tttttggttt agataaaaag gatgtcaaaa atgtctgatg taccgttgat
     5761 tgctgttgta atggctggtg gtacgggtag ccgtctttgg cccctctcaa gagagcacta
     5821 tccgaagcaa tttctgcaat tatcaggtga gaatacttta ttacagtcaa ctttattacg
     5881 actatccccg ctttcttgcg aaactccatt agtaataact aacgagcagc atcgttttgt
     5941 ggtcgcagaa caattgcgtc aaataaacca attaagcgat aacattattc ttgaaccatg
     6001 tggaagaaat actgcaccag ctatagcact ttcagcattt acagccttaa agcgtaatga
     6061 acaacaagat cctatacttt tagttcttgc agctgaccat gttataaaca aaacagatgt
     6121 tttttgtaat gctattaaaa attccatttc aattgttgaa caaggaaata ttttaacatt
     6181 tggtattatt cctgattatg cagaaaccgg ttacggttat atagaaaaag ggagtatagt
     6241 taaagaatca caacgtggag ttggtaatac attctatcac gtggaacaat ttgttgaaaa
     6301 acctaatcgc tcaagggctg aagagtatat ctcctcaggt aaatatctct ggaatagtgg
     6361 gatgtttatg ttcaaagcat cagtatattt agaggagtta aaaaaataca gacctgatat
     6421 atacgatata tgtgaaaaaa caatttcttc gtcctaccat gatcttgatt ttatacgact
     6481 ttcgaaagat gtttttcaaa attgtccatc ggaatcaatt gattttgcag taatggaaaa
     6541 aacaaagcgt tgtatagtct atcccgttga tattggttgg aacgatgttg gatcttggca
     6601 atcattgtgg gacgttagtg ataaaacccc tacaggggac gtttgcaaag gtgatatcct
     6661 gacctataat acaaaaaata attatattca ttcggagtca gccttagtag ccgctgtagg
     6721 tgtggaagat attgttattg ttcagacgaa agatgcaatt ctagtttcaa aaaaatccga
     6781 agttcaggat gttaagaaaa ttgttcaaat gcttaaaatg caagaacgtt ctgaatatct
     6841 atcacatcgc gaggagtttc ggccttgggg aaagtttgat gctatagaac agggagatcg
     6901 atataaggtc aaaaagattg tcgttaaacc aggggaaggg ctatccttac gtatgcatca
     6961 tcatcgttca gagcattgga ttgtcctttc aggtacagca aaagtaacgt taaataacaa
     7021 aactttctta gttactgcta atgaatccgt ttatattcct ctgggtgcta cttatagcct
     7081 tgaaaatcca ggtataattc ctctcaatct tattgaagtt agttcaggag attatttagg
     7141 tgaggatgat attgttcgtc aaaaagaacg ttacaaaata gatgactaat aaatgaaaaa
     7201 attaacctgc tttaaagcct acgatattcg cgggaaatta ggcgaagaat tgaatgaaga
     7261 tattgcctgg cgcattggtc gcgcttatgg cgaatttctc aaaccgaaaa ccattgtgtt
     7321 aggcggtgat gtccgcctca ccagcgaaac cttaaaactg gcgctggcga aaggtttaca
     7381 ggatgcgggc gtcgatgtgc tggatattgg tatgtccggc accgaagaga tctatttcgc
     7441 cacgttccat ctcggcgtgg atggcggcat tgaagttacc gccagccata atccgatgga
     7501 ttacaacggc atgaagctgg tgcgcgaagg ggctcgcccg atcagcggcg ataccggact
     7561 gcgcgatgtc cagcgcctgg cagaagccaa cgactttcct cccgttgatg aaaccaaacg
     7621 cggtcgctat cagcaaatca acctgcgtga tgcatacgtt gatcacctgt tcggttatat
     7681 caacgtcaaa aacctcacgc cgctcaagct ggtgatcaac tccgggaacg gcgcagcggg
     7741 tccggtagtt gacgccatcg aagcccgctt taaagccctc ggcgcacccg tggaattgat
     7801 caaagtgcac aacacgccgg acggaaattt ccccaacggt attcctaacc cgctgctgcc
     7861 ggaatgccgc gacgacaccc gcaatgcggt catcaaacat ggcgcggata tgggcattgc
     7921 ttttgatggc gattttgacc gctgtttcct gtttgacgaa aaagggcagt ttatcgaggg
     7981 ctactacatt gtcggcctgc tggcagaagc gttcctcgaa aaaaatcccg gcgcgaagat
     8041 catccacgat ccgcgtctaa tttggaacac cgttgatgtg gtgagtgccg caggtggcac
     8101 cccggtaatg tcgaaaaccg gacacgcctt tattaaagaa cgtatgcgca aggaagacgc
     8161 catctacggt ggcgaaatga gcgcccacca ttacttccgt gatttcgctt actgcgacag
     8221 cggcatgatc ccgtggctgc tggtagccga actggtgtgt ctgaaaggaa aaacgctggg
     8281 cgaacttgtg cgtgaccgga tggcagcttt tccagcaagt ggggaaatta atagcacgtt
     8341 ggaagatcca ttatgtgcta tcgcacgggt tgaaagttat tatgcaaata aggcaattga
     8401 gatcgatcgt acagatggta taagtatgac ttttaatgga tggcgcttta acttacgttc
     8461 ttctaatacg gaacccgtag tccggttaaa cgttgaatct agaggagatg tggccgcaac
     8521 taaaaaatat acaggcttca tattagactt attaaattat aaaaagtcaa tatcatctta
     8581 acaagtaaat gtgagttatt atgataaagt tgctttttag ttcatcttta ttaaagatgg
     8641 gtgttgtttt attaaatttg cttattcctg caataataat acgattctat tcagagtatg
     8701 attttggaat ttattcttat ttactaacat acgcaatcgt tatttctgtt ttacaaaacg
     8761 gcatcactgc aagcttccgt aatctgatat caaatttaga aaaaaatgat ttgcttgatt
     8821 atttattata ttcagtgagg aaaataacgt atccattctt attaataata ttttgtttat
     8881 tattggctac gctttatgta gttgggtttg atacgttata tggtaaaata tgttttttta
     8941 ttgctgtatc gttaataaat atatattatc cagtggttgc ttcatattat gatgctacag
     9001 gtaaaacgct caattttgta ctacttgaaa taatattttt actcgtattc attgcaggcg
     9061 tgggcatttt aatttactac aaagtgagta tttacattgt ttgcatttta actgctaact
     9121 acagaggggt ttatgcaata ttattaataa tgaagaagtg tgttattttt aaacaaataa
     9181 ggcttaagaa aaaggggggg tataaaattt tttgttctga tgatatgatt tttattataa
     9241 ttcaattaat aaatgttagt aatacattat tatttatgaa cgtgtttgca aaattttacg
     9301 gtgtagtggc gtttggggta ttttcagtat attacagatt tgttgctttt ccccaacaag
     9361 tgataagctt ttcctcgtcg ttaatatgga ttaactttcg tcaagtccat gttaataaca
     9421 gggttgctgc catgcaacta ttaaagagat tattttttat ttttactcta ttgcttatta
     9481 tatggggagg gtgcgttcat ttttttatag acaaagtcgt atatctttat tcatccaaac
     9541 ctttagaata cccaggtgtg ttgtgttttc taaatattat ggtttgcttg atgttgctaa
     9601 aagacttttc aagtataata ctcaatgcat taactttata taaagaacaa atgataatga
     9661 atgctttact ttgtttacta aacgttattt ttttcttttt ttataatgaa acgaattttg
     9721 ataccatata tctaatcttc gtttcactgt taacattgat gtttgtattt gtaaatttat
     9781 cactaattaa aagcaggctc tgaagatggt tattaatata ttttatatat gcacaggcga
     9841 atataaaagg ttttttgata aattttattt atcttgtgag gataaattta taccagagtt
     9901 tgaaaaaaaa tattatgtgt ttaccgattc tgataggatt tattttagta aatatctgaa
     9961 tgttgaagta attaatgttg aaaaaaattg ctggccgctt aatacgttat tacgtttttc
    10021 atattttttg aaggttattg ataagttaca aactaactca tatacttttt tctttaatgc
    10081 aaatgcagtt attgtcaaag agattccttt tagtacattt atggagagtg atttaatcgg
    10141 tgttattcat ccagggtata agaatcgtat atcgattctt tacccttggg aaagaagaaa
    10201 aaatgcgacc tgctatcttg ggtatttaaa gaaaggtatt tattatcaag gttgtttcaa
    10261 tggaggtaaa acagcgtcat ttaagagatt aatacaaatt tgtaatatga tgacaatggc
    10321 tgatttgaaa aaaaacctga ttgctaaagt acatgatgag tcatatttga attattatta
    10381 ttactacaat aagccactat tactttctga attgtactca tggcctgaaa aatatggtga
    10441 aaataaagac gccaagatta ttatgcgtga taaggaaaga gaaagttggt atggtaatat
    10501 caagaagtaa ttatagaaat atatgttcat atactttttt tatggtgaat ttatttatct
    10561 taattttatc cgtaatcaat gaaggttttt gtgagattgc ctatgtaata ataagtgttt
    10621 cttctgttct tttttgtgta atcataatct gccttgaaag gcaagggggc tttctcaacc
    10681 caatgacttt ctgtattata tctgtctttt tctttatctt aatcagacct gtcttttttt
    10741 cgcaaaatat aacagagaat ttaaacgaag taataactgc tggtttagaa attgatgaga
    10801 tatatgtttt ttattcgtta gctgttgtaa acattccctt ggcttttact gtattactgt
    10861 attcagtaca aaaaggtact gtttccaagt tagtagggca gttacctgat ttgttttttt
    10921 ataataaaca attgtcaatg attttattat ggggggggct tttctctgct attttcttaa
    10981 ttaaatcata caaaaaattt attatattag gtcaggtttc agtatttgag gctgacgcgt
    11041 atggactata tgatgagtta ttttggttca ccctttcaaa atattgttat atattgtctt
    11101 tgcttttttc taaaaataaa aatttcatac tatattcttt actgatattt ataacatcga
    11161 taggttacat tttagtggga ttaagaggct atacaatcgc atatggtttc ttattgttat
    11221 tttttctaga tataagatat aggcttaaga tcaaatggtt gttgttagta gctatattgg
    11281 taacgaccat ttcttcactg ttccttaact atagaatagg tatagaagta aatagcggat
    11341 tattaggaat tatttttaat ccattattac agcaaggtgc ttcatttgaa accgtttatg
    11401 gtgctttgaa atacaatgaa aaaatattaa gttgcatatc ttactatgat tattttttta
    11461 caaataaaga tatcggttct tgtattgaca tcgctcgtgg tgtttatttt aaagaaggag
    11521 gaagtttcgc atcaagtttt tacagtgagc taatatattt ggggtggatc attggaagtg
    11581 ttgctttatt actatttgca ttttctctag cttttgttca atcttgttat gagaaaataa
    11641 taaaaaattc aatgaataat aaattggcat atacatatcg tttgatcatt tttttagcgc
    11701 taccaaatct tatatatttt gcccgctcat cattatttga ttttattact aaggttttat
    11761 ttatagctct attcataggt gggctaagca tagtgagaca tattgcttta aatataaaaa
    11821 aatgtcattg agaatattag atatgatttc agtaataatg gctgtacacc gatatgataa
    11881 atatgttgat atttcaattg atagtatctt aaatcagaca tactctgact ttgagttaat
    11941 aataattgca aatggagggg attgtttcga gatagcaaaa cagctgaagc attatacaga
    12001 gctggataac agagttaaaa tttatacatt agaaataggg cagttatcgt ttgcattaaa
    12061 ttacgcagta actaagtgta aatactctat tattgccaga atggattccg acgatgtttc
    12121 actgccgtta cgtctagaaa aacaatatat gtatatgttg cagaatgatt tagaaatggt
    12181 ggggactggg atcagactta tcaatgaaaa cggtgagttt attaaagaat taaaatatcc
    12241 aaatcataat aagataaata agatacttcc ttttaaaaat tgttttgcgc atcctacttt
    12301 gatgttcaag aaagatgtta tactaaagca gcgaggttat tgtggtggtt ttaattcaga
    12361 agattatgat ctatggctca gaatcttaaa tgaatgtccg aatatacgct gggataatct
    12421 aagtgagtgt ttgctaaatt atcgaattca taacaaatct acgcaaaaat cagcactcgc
    12481 atattatgaa tgtgctagtt attctctgcg agaattctta aaaaaaagaa ctattacgaa
    12541 ttttctttct tgcctctatc atttttgtaa agcactaata aaataaaggt tgacgctatg
    12601 tatagttgtt tgtctggtgg attaggtaat caaatgtttc agtatgctgc ggcatatatc
    12661 ttacagagaa agcttaaaca aagatcatta gttttagacg atagctattt tttagattgc
    12721 tcaaatcgtg atacacgtag aagatttgaa ttgaatcaat ttaacatatg ttatgatcgt
    12781 ctgactacaa gtaaggaaaa aaaagagata tccataatac gacatgtaaa tagatatcgt
    12841 ttgcccttat ttgttacaaa ttctatattt ggagttctac taaaaaaaaa ctatttgcct
    12901 gaagcaaaat tttatgaatt tttgaacaac tgtaaattac aggttaaaaa tggttattgt
    12961 ctattttctt atttccagga tgctacattg atagatagtc atcgtgatat gattctccca
    13021 ttattccaga ttaatgaaga tttgctcaat ttatgtaatg acttgcatat ttacaaaaaa
    13081 gtgatatgtg agaatgctaa cacaacttca ctacatatca ggcgtggaga ctacatcacc
    13141 aaccctcacg cctctaaatt tcatggggtg ttgcccatgg attactatga aaaggctatt
    13201 cgttatattg aggatgttca aggagaacag gtgattatcg tattttcaga tgatgtgaaa
    13261 tgggctgaga atacatttgc taatcaacct aattattacg ttgttaataa ttctgaatgc
    13321 gagtacagtg cgattgatat gtttttaatg tcaaagtgta aaaacaatat aatagccaat
    13381 agtacatata gttggtgggg ggcatggtta aatactttcg aagataaaat agttgtttcc
    13441 cctcgtaagt ggtttgctgg aaataataaa tctaagttga ccatggatag ttggattaat
    13501 ctttgattat gctgtgtgtt ttttttaaaa tatgatatcc attcataatt tgtaattttg
    13561 atatattatt tttagactaa aatgtcaatc tgcaacattc cgtagtaatc cctgacagga
    13621 gtaaacaatg tcaaagcaac agatcggcgt agtcggtatg gcagtgatgg ggcgtaacct
    13681 tgcgctcaac atcgaaagcc gtggttatac cgtctctatt ttcaaccgct cccgtgagaa
    13741 aacggaagaa gtaattgccg aaaacccggg caagaaactg gttccttact atacggtgaa
    13801 agagtttgtt gaatctctgg aaacgcctcg tcgcatcctg ttaatggtga aagcaggtgc
    13861 aggcacggat gctgctattg attccctcaa gccatacctc gataaaggtg acatcatcat
    13921 tgatggcggt aacaccttct tccaggacac cattcgtcgt aaccgtgagc tttctgccga
    13981 aggctttaac ttcattggta ccggtgtttc cggtggtgaa gagggcgcgc tgaaaggtcc
    14041 ttccattatg cctggtggcc agaaagaagc ctatgaactg gttgctccga tcctgaccaa
    14101 aatcgccgca gtagctgaag acggtgagcc atgcgttacc tatattggtg ccgatg
//
