LOCUS       AY035396               10196 bp    DNA     linear   BCT 03-NOV-2002
DEFINITION  Escherichia coli O91 O-antigen gene cluster, complete sequence.
ACCESSION   AY035396
VERSION     AY035396.1  GI:24527246
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10196)
  AUTHORS   Perelle,S., Dilasser,F., Grout,J. and Fach,P.
  TITLE     Identification of the O-antigen biosynthesis genes of Escherichia
            coli O91 and development of a O91 PCR serotyping test
  JOURNAL   J. Appl. Microbiol. 93 (5), 758-764 (2002)
   PUBMED   12392520
REFERENCE   2  (bases 1 to 10196)
  AUTHORS   Perelle,S., Dilasser,F., Grout,J. and Fach,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2001) LERHQA, AFSSA, 1 #5 rue de Belfort,
            Maisons-Alfort 94700, France
FEATURES             Location/Qualifiers
     source          1..10196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ECA95"
                     /serotype="091"
                     /db_xref="taxon:562"
                     /note="Shiga toxin-producing Escherichia coli (STEC);
                     assignment of these genes was in accordance with the
                     Bacterial Polysaccharide Gene Database (BPGD)"
     gene            57..1133
                     /gene="rmlB"
     CDS             57..1133
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="DTDP-glucose 4,6-dehydratase"
                     /protein_id="AAK60448.1"
                     /db_xref="GI:24527247"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
     gene            1130..1993
                     /gene="rmlA"
     CDS             1130..1993
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="putative D-glucose-1-phosphate
                     thymidylyltransferase"
                     /protein_id="AAK60449.1"
                     /db_xref="GI:24527248"
                     /translation="MKGIILAGGSGTRLYPITMGISKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILIITTPEDQFSFIRLLGDGKHLGVSISYAVQPRPDGLAQAFTIGKDFIGADSV
                     CLVLGDNIFFGQGFTPKLKTACERKLGATIFGYQVRDPGRFGVVEFDSDYRVLSIEEK
                     PLKPKSNWAVTGLYFYDNDVVEIAKSITPSQRGELEITSINEVYLKNNKLTVELLGRG
                     FAWLDTGTHDSLIEAGSFVETVQKRQGMMVACPEEIAWRNGWLNNSDLKSLATNMKNS
                     YGSYLTKLILQ"
     gene            2023..2787
                     /gene="wbsA"
     CDS             2023..2787
                     /gene="wbsA"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAK60450.1"
                     /db_xref="GI:24527249"
                     /translation="MNDKSITPLISVITATFNSQRFILDTYKSLQAQSYKNWEWVVTD
                     DASEDKTVSILKELQSNDDRIKIFINADNKGAAWSRNNSMKESKGQYFAFIDSDDLWH
                     PEKLSRQIAFMGDNIDFSFTSYELVDVENKKLNKKIDITPILPLTYHQMLRKKATLGC
                     STVILRVSAFPDVRMPDLRTGQDYATWLKILKRGTRAYLLPEVLTSYRIVNGSISRNK
                     FKKALRQWEIYRKYENLSFLYSCYCFSLYAFRAVVR"
     gene            2792..3196
                     /gene="wbsB"
     CDS             2792..3196
                     /gene="qdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="unknown"
                     /protein_id="AAK60451.1"
                     /db_xref="GI:24527250"
                     /translation="MNINLIELQQHGDERGALVSLECNKNIPFDIRRVYYIYNTNKNV
                     VRGYHAHRKLKQMAICVKGYCTFTLDDGFERISIKLDNPAQGLLIESCLWREMSDFSE
                     DCVLLVLADADYDELDYIREYETFKKIVRGIT"
     gene            3193..3732
                     /gene="wbsC"
     CDS             3193..3732
                     /gene="wbsC"
                     /codon_start=1
                     /transl_table=11
                     /product="putative butyryltransferase"
                     /protein_id="AAK60452.1"
                     /db_xref="GI:24527251"
                     /translation="MIMHKGKTIYLSSVTVDVESARKIVELRNGDVQKKFLNPTKNSI
                     EDQLEWLGNYLERNKNGDEFYFFIKRIDTDEIIGTVRIYDIKRDIDSFCWGSWILNER
                     KTHTAALESYLLINKFAFYHLNYQNSHFDVRKDNSKVISFHKKTGAVITDDDEMNYYF
                     QYTKEKFEKFSLKYNGLIK"
     gene            3729..4841
                     /gene="wbsD"
     CDS             3729..4841
                     /gene="qdtB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminotransferase"
                     /protein_id="AAK60453.1"
                     /db_xref="GI:24527252"
                     /translation="MKKIDFLNLKMMNQPYRADILNAITKVVDSGWYIQGEEVRNFEH
                     EFASFIGSKYCVGVANGLDALSITLKAWILLGKIKEGDEVIVPANTFIATVLAITQNH
                     LKPIFVEPDEETFNINVDMIRNVITEKTKVIIPVHLYGQVAEMNKIRELADLHDLLIL
                     EDSAQAHGAMYNNLKAGNLGDAGAFSFYPGKNLGALGDAGAVVTDNQELAETIRAIGN
                     YGAHVKYKHDVQGVNSRLDEIQAAILRVKLKYINNEIESRRIIANNYLTKIINPIIRL
                     PKIDNNAELSHVWHLFVIKTNERNKLQDYLAKHSVQTLIHYPIPIHKQLAYNNLNTYS
                     YPITEKLHDEILSLPIGSFLSIEDADYIVSLINEFE"
     gene            4847..6112
                     /gene="wzx"
     CDS             4847..6112
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AAK60454.1"
                     /db_xref="GI:24527253"
                     /translation="MRMLKTGLLNFISVLVKVITGLFLNKIMALYVGPAGYALVSQFQ
                     NGINFITTFASGAINTGVTKYTAEYANDKGKTSDLFKCITLLAITCCSFFSLTIIVFS
                     EYFSLKLLGTINYSGVIKWFAATLFLFVANSILVSIINGLKEFKLYVLINIFGSLFSL
                     LFSGILIYFFNLKGALIAFATNQSIVFFMTFYLIISKKMVSLKSFIGMPSDFYFIKKL
                     IQYALMAIVSAFCVIYMQIIIRTYIANHFGWVQAGIWDGVWRISSLYLMLVTVPLGVY
                     YLPRLAELHTKSLLLREIKQGYLFLLPIIIISAIIIYYLRYFIIELLFSKSFVSMGDI
                     IGWQLLGDIFKIGSWLLSYIMLSKALTKTYIITEITTALLFLGGVHYLSPLYGLKGVT
                     LAYCITYFCYWIMMIVVIISYFKGNFRNE"
     gene            6105..7274
                     /gene="wbsE"
     CDS             6105..7274
                     /gene="wbsE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAK60455.1"
                     /db_xref="GI:24527254"
                     /translation="MNSFRILLVGDFSGVHSTLREALRKKNFDVTLISDGDSYKDLPS
                     DIVFGKVTKNNDNRGFLKKICNYVLTLLGVNGIGVYIKNRYKIKQLSSFDVIQLINPQ
                     PFQEIGWLINLYIIYKLNRKTKKMYLCALGEDYYWVKSCIKKRFKYSALDNVPGNKRK
                     YRYSLKFLKLYYKVLNDYCVAISEKIIPGLVDYRIAYEHFDKCTDVIPLPINKNFVTT
                     KSLSLKNDDKIVIFHGWQVGKELKKGNYIFDRAIKKLQEKYNEQIEYVIVKNVPYDEY
                     VKSFSRAHLFIDQCYSYDKGVNALLGMAAGKVVFSGFEPEAKRYYEFFDDKYTPLINA
                     VPDEDTIFLQIEDLLLNKQKINNISAAAREFILRYHLSEDVADNFLNVWGYDIHD"
     gene            7246..8121
                     /gene="wbsF"
     CDS             7246..8121
                     /gene="wbsF"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAK60456.1"
                     /db_xref="GI:24527255"
                     /translation="MFGDMISMTKFSLVLCTINNRKDIPKFIESLSCNIHEKKFEVEL
                     IVIDQNTLSIADLFSDIAERVTLKYFKVDFKGLSRARNYALKHVSGDIVCFPDDDCIY
                     TQSLLKNIYELFAYNNHVDFISVNTKDPIAGEKALVKLPKSKTNISFKSKNCVSFTLF
                     FRREVINAVGRFDQNMGVGAGTNYGAGEESDFVVRALSLGFKGLYYPDYYVLHPAKES
                     HPVFDDIIKNRMLSYGGGYGYFLQKNFYRQGRFLSIKNIFGVFLRLIKSLHSKVEFLK
                     SFYFTIGFLKGFFKK"
     gene            8118..8915
                     /gene="wbsG"
     CDS             8118..8915
                     /gene="wbsG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AAK60457.1"
                     /db_xref="GI:24527256"
                     /translation="MKIDILLSTYGDRILGINRLIQSIDFSNCNLIIAHQFKNKISSQ
                     AKTFIQKLLHENNNISYYQLNSFGVTKSRNFLINKAKSNYIAFCDDDIYYFPNAISTV
                     LDKMEHENIDVATCMTITDDSKPLKNYASRDYHHNKRTILRVGTVEIICKRESILKTD
                     LFPEDMGAGALFPLCDEPVFLNNCLKQSLKVQFLSIPLCYHPNVSSGSNLNRCYTIAR
                     GVCFRRIYGMPALLLILIFSLKMKLKYRKLDLLTYTKSLYRGYNEGK"
     gene            8902..10113
                     /gene="wzy"
     CDS             8902..10113
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AAK60458.1"
                     /db_xref="GI:24527257"
                     /translation="MKASSVLTPYLNWFYGFLCFLSLWPFFSWKWPAIPVSFIAICFF
                     LFLFDLCYINKLKVNKKIFLIIFSTLFVLILFILLPGGIPPWFNYYSLFLFLFLLLPR
                     KRIFEISLKFRSIFIFSLLPGLVIYALLIIGVKLPYGILDAHNELKDSLGIYYRDYIG
                     TVALSHLVLTVGDSTIIRFSGMLDEPGLLGTISALLLLADKLNFKHKSNYVLLLSGVI
                     SISLAFYLLILMGLIFQTRRKIISLAIVFLIGIAIYHSPFYENFLQKRISDSESGLVF
                     RDNRISDCFKEYYTAFENDSELNLFFGIGNNAHLRTGCDVSSYKMYIYDYGYLGFITI
                     ALLQIMQYMIPIMGGYACKFLKASFVFICLFILSYYQRPIFFNLAFSMIFYYSIFTYL
                     KTVGISATNKK"
BASE COUNT     3367 a   1408 c   1918 g   3503 t
ORIGIN
        1 caaacaaaga gccgcttatt tcacagcatg ctctgatgta atatggaata aattaagtga
       61 aaatacttgt tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa
      121 ataatacgca ggatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctggaat
      181 cacttgctga tgtttctgat tctgaacgct atatttttga acatgcggat atttgcgatg
      241 ctgctgcaat ggcacggatt tttgctcagc atcagccgga tgcagtgatg cacctggctg
      301 ctgaaagcca tgtggatcgt tcaattacag gccctgcggc atttattgaa accaatattg
      361 ttggtactta tgtccttttg gaagccgctc gcaattactg gtctgctctt gatagcgaca
      421 agaaaaatag cttccgtttt catcatattt ctactgacga agtatatggt gatttgcctc
      481 atcctgacga ggtaaataat acagaagaat tacccttatt tactgagaca acagcttacg
      541 cgccaagcag cccttattcc gcatccaaag catccagcga tcatttagtc cgtgcgtgga
      601 aacgtaccta tggtttaccg accattgtga ctaattgttc gaataactac ggtccttatc
      661 actttccgga aaaattgatt ccactagtaa ttcttaatgc tctggaaggt aaggcattac
      721 ctatttatgg caaaggggat caaattcgcg actggctgta tgttgaagat catgcacgtg
      781 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca
      841 acgaaaagaa aaacatcgat gtagtgctca ctatttgtga tttgttggat gagattgtac
      901 cgaaagagaa atcttatcgt gagcaaatca cttatgttgc cgatcgtccg ggacacgatc
      961 gccgttatgc gattgatgct gagaagatta gccgcgaatt gggatggaaa ccacaggaaa
     1021 cgtttgagag cgggattcgt aaaacaatta actggtattt gaataataat gattggtgcc
     1081 ggcgagttca agatggctct tatcaacgtg aacgtttggg ggtaattaga tgaaaggaat
     1141 tattttagct ggtggttctg gaacaaggct ttatccaatt acaatgggga tttcaaagca
     1201 attattgcct gtttatgaca agccaatgat atattatcca ttgtctgttc tgatgttggc
     1261 agggatcaga gatatactta ttattacaac gcccgaagac caatttagtt ttatacggtt
     1321 gcttggtgac ggaaaacatc taggtgtcag cattagttat gcagtgcaac caaggccaga
     1381 tggtttagcg caagctttca caattggaaa agattttata ggtgctgatt cagtttgcct
     1441 tgtattaggt gataatattt tttttggtca agggtttaca ccaaaattaa aaactgcatg
     1501 tgaaaggaaa ttaggagcca caatttttgg ctatcaggta agagatccag gacggtttgg
     1561 agttgtagag tttgactcag attatagggt attaagtatt gaagaaaaac cgttaaaacc
     1621 aaaatcaaac tgggctgtaa ctggacttta tttttatgat aatgatgttg ttgaaattgc
     1681 caaatcaatt actccttctc aacgaggtga gctagaaata acttcgataa atgaagttta
     1741 tctaaaaaac aacaagttga cggtcgaact gcttgggcgt ggtttcgcat ggttagatac
     1801 aggcacgcac gatagcctaa ttgaggcggg aagtttcgtt gaaactgtac aaaaaagaca
     1861 gggtatgatg gtagcgtgtc ctgaagaaat tgcttggcga aatggttggt taaataattc
     1921 tgatttgaaa tcattagcaa ctaatatgaa aaatagttat ggttcttatt taacaaaact
     1981 aattttacaa taaataatta tgcaacgaat aacataatac gtatgaatga taaaagtatt
     2041 acaccattaa tttcagttat tacggctaca tttaattctc agcgttttat tctggatact
     2101 tacaaaagtc tccaggcgca gagctataaa aactgggaat gggtcgttac cgatgatgcc
     2161 tcagaagata aaacggtaag tattttaaaa gagttacagt caaatgatga caggataaaa
     2221 atatttatta atgctgacaa caaaggagct gcgtggagta gaaataatag tatgaaggaa
     2281 agtaaaggtc aatattttgc tttcattgat agtgatgatc tatggcatcc agagaagttg
     2341 tctaggcaaa tagcattcat gggtgataat attgattttt cttttaccag ttatgagttg
     2401 gtagatgttg aaaataaaaa attaaataaa aagatagata ttacacctat attaccttta
     2461 acttatcatc agatgttaag gaaaaaagct actttaggtt gttcaactgt tatattgcgt
     2521 gttagcgctt ttcctgatgt tcgtatgccg gacttaagaa ctggacaaga ttatgctact
     2581 tggttaaaaa tacttaaaag aggcacaaga gcatacttgc ttcctgaggt attaacatct
     2641 tatagaattg tgaatggttc aatatcaagg aataaattta aaaaagcatt aaggcaatgg
     2701 gaaatttata gaaaatatga aaatttgagc tttttatata gctgctattg tttttccctt
     2761 tatgcgttca gagctgtagt taggtaagaa aatgaatata aatttaattg aacttcaaca
     2821 gcatggtgat gagagagggg cattagtatc tcttgagtgt aacaaaaata tccccttcga
     2881 tataagacgt gtttattata tatataatac aaataaaaat gttgtcagag gttaccatgc
     2941 acatagaaag ctgaaacaaa tggcgatttg tgtgaagggc tattgtactt ttactttaga
     3001 tgatggattt gaacgaatat ctattaaatt agataatcct gcgcaaggtt tgctaattga
     3061 gtcttgctta tggagagaaa tgtctgattt ttcagaggat tgtgtgttgt tagtattagc
     3121 agatgcagat tatgatgaac ttgactatat tagagaatat gaaacattta aaaagattgt
     3181 tagggggata acatgattat gcataaagga aaaacaatat atctatccag tgtcacagta
     3241 gatgtcgaaa gcgcacggaa gatagtagag ctaaggaatg gcgatgtgca aaaaaaattt
     3301 ttaaatccca ctaagaattc tatcgaagat caacttgaat ggttagggaa ttatttagaa
     3361 agaaataaga atggagatga attttatttt tttattaaac ggattgatac tgatgagatt
     3421 ataggaacgg ttaggatata tgatattaaa cgtgatatag attctttttg ctggggtagc
     3481 tggatcttga atgagcgtaa aactcacacc gctgcattag aaagttattt actgataaat
     3541 aaatttgcgt tttatcattt aaactatcag aattcacatt ttgatgttag aaaagataac
     3601 agtaaggtaa tttcatttca taaaaagaca ggtgcagtta taacagatga tgacgaaatg
     3661 aattattatt ttcagtatac aaaagaaaaa tttgaaaaat tttccttaaa atataatggg
     3721 cttattaaat gaaaaaaata gacttcctaa atctaaaaat gatgaatcaa ccttatcgag
     3781 cggatatact aaatgcaata actaaagttg ttgattcggg gtggtatatt caaggtgaag
     3841 aagtcagaaa tttcgaacat gagtttgcat catttattgg ttcgaagtat tgtgtcggtg
     3901 ttgccaatgg cttagatgca ttgagcatta ccttaaaagc atggatatta ttaggcaaaa
     3961 ttaaagaagg ggatgaggta atagttccag caaatacatt tattgcaact gtattagcta
     4021 tcacccaaaa tcatcttaaa ccaatatttg tagaaccaga tgaagaaaca ttcaacatta
     4081 atgttgatat gattagaaat gtcatcacgg agaagactaa agttattata cctgttcatc
     4141 tttatgggca ggtggcagaa atgaataaaa ttcgtgaatt agctgacctt catgatctgt
     4201 tgattttgga ggactctgcg caagcacatg gagccatgta taataacctt aaggcaggaa
     4261 accttggtga tgcaggcgct tttagtttct acccaggtaa aaacttgggg gcattagggg
     4321 atgctggtgc tgtagtgact gacaatcaag agctggcaga aactataaga gcaattggta
     4381 attatggtgc acatgtaaaa tataaacatg atgtacaagg ggttaatagc cgtctagatg
     4441 agattcaagc agcgattcta cgggttaaat taaaatatat aaacaatgaa atagaatcta
     4501 gaagaataat agcaaataac tatttaacaa aaataatcaa tccaattatc agattgccta
     4561 aaatagataa taatgcagaa ttatcacatg tatggcatct ctttgttata aaaacaaatg
     4621 aaaggaataa actacaagat tatttagcta aacatagtgt tcaaacattg atccattatc
     4681 caattcctat acataagcag ttagcgtata ataatctgaa tacatattct tatccgataa
     4741 ctgagaaatt acatgatgaa atattatcac ttccaattgg tagcttttta tctattgaag
     4801 atgctgacta tatagtatca ttaattaatg aatttgaata gattatatgc gcatgttaaa
     4861 aaccgggtta ttaaatttca tatctgtatt agtcaaagtt ataactggtt tatttctaaa
     4921 taaaatcatg gccctttatg tagggccagc tggatatgca ctagtaagtc aattccaaaa
     4981 tggaattaat tttattacaa catttgcatc tggcgcaata aacacgggag ttacaaaata
     5041 tactgcagaa tatgctaacg ataaggggaa aacctctgat ctatttaaat gtataacctt
     5101 gttagctata acatgctgct cattcttctc attaacaata attgtttttt ctgaatattt
     5161 ttcgttaaaa ttattgggca ctattaatta tagtggtgta attaaatggt ttgctgcgac
     5221 gctatttttg tttgttgcaa actccatatt ggtttcaatt ataaatggtt taaaggaatt
     5281 taaactttat gttctaataa atatatttgg aagtttattc tcactgttgt tctcaggaat
     5341 attaatctat ttttttaatt taaaaggagc tcttatcgca tttgccacaa atcaatcaat
     5401 tgtttttttt atgacgtttt atcttataat tagtaaaaag atggtgtcat taaaatcttt
     5461 cattggcatg ccgtcagatt tttattttat taaaaaatta atacaatatg cattaatggc
     5521 tatagttagt gcattttgtg ttatatatat gcagattatt attagaacat acatcgcgaa
     5581 tcattttgga tgggtgcaag caggtatttg ggatggtgtc tggcgtataa gttcattata
     5641 tttaatgtta gtcaccgttc ctctaggggt atattatctc ccaaggcttg cagaactaca
     5701 tactaagtcg ttgcttttaa gagagattaa acaaggatat ctgtttcttc ttccaattat
     5761 aattatttca gctattataa tttattatct tagatatttt attattgaat tgcttttttc
     5821 aaagtcattc gtatctatgg gggatattat tggctggcag ttacttggtg atatatttaa
     5881 aattggaagt tggctacttt cttacattat gttgagtaaa gcattaacga agacatatat
     5941 tattacagag attacaactg cattactttt tttaggcgga gtacactatc tttcgccttt
     6001 atatggttta aaaggtgtta ctctggcgta ttgtataacc tatttttgtt actggattat
     6061 gatgatagtt gtaataattt cttattttaa ggggaacttc agaaatgaat agctttagaa
     6121 tattgctcgt tggtgatttt tctggagtcc attcaacctt aagggaggca ttaaggaaga
     6181 agaattttga tgtaactctt atttctgatg gggattctta taaagattta ccatcagata
     6241 tagtatttgg aaaggtgact aaaaataatg ataatagagg cttcctgaaa aaaatatgta
     6301 attatgtatt aaccttgctg ggtgttaatg gtatcggtgt atatatcaag aatagatata
     6361 aaataaaaca attatctagt tttgatgtaa ttcaacttat aaaccctcag ccatttcaag
     6421 aaattggatg gctaataaat ttgtatatta tttacaaact gaatagaaaa acaaaaaaaa
     6481 tgtatttgtg tgctctaggt gaagattact attgggtgaa atcatgtatt aaaaagcggt
     6541 ttaagtatag tgctttagat aatgtccctg gtaataagag aaaatacaga tactctctca
     6601 agtttttaaa gctttattat aaagtgctaa atgattattg tgttgctatt tcagaaaaaa
     6661 taataccagg cctagtagac tatagaattg cctacgagca ttttgataag tgtactgatg
     6721 ttatcccact accgataaat aaaaattttg ttactacaaa gtctttatcg ttaaaaaatg
     6781 acgataagat tgtgatcttc cacggctggc aagttggtaa ggaattaaaa aaagggaatt
     6841 atatctttga tagggctata aaaaaattac aagaaaaata taatgaacag atcgaatatg
     6901 ttattgttaa aaatgtacct tatgatgagt atgttaagtc tttttcacgt gcgcatcttt
     6961 tcattgatca gtgctacagt tacgataaag gagtcaacgc attactcgga atggctgcgg
     7021 gaaaggtggt ttttagcggc ttcgaaccgg aggcaaaacg atattatgaa ttttttgatg
     7081 ataaatacac accattaata aatgctgttc cagacgagga tactattttt cttcaaatag
     7141 aagatttgtt attaaataaa caaaaaataa ataatatttc agcagcagca agagagttta
     7201 tcctacgtta ccatttgtct gaagatgttg ctgataattt tctgaatgtt tggggatatg
     7261 atatccatga ctaaattttc ccttgtctta tgcactataa ataataggaa agatatccca
     7321 aaatttatcg aatcattatc atgtaatatt catgaaaaaa aatttgaagt agagttaatt
     7381 gttatagatc aaaatacatt gtcgattgcg gatttatttt ctgatatagc tgaaagagtg
     7441 actttaaaat actttaaagt agatttcaaa gggttgtcac gtgcaagaaa ttatgcttta
     7501 aaacatgttt caggcgatat tgtatgtttt ccagatgatg actgcattta tacacagagt
     7561 ctactaaaaa atatttatga gttatttgca tataataatc atgttgattt tatcagcgtt
     7621 aatactaagg atcccattgc aggggaaaaa gcactggtca agctacctaa aagcaagact
     7681 aatatttcat tcaagagtaa aaattgtgtt tcatttacat tatttttcag gcgcgaagtt
     7741 ataaatgctg ttggtagatt tgatcaaaat atgggggtcg gagctggcac taactatggt
     7801 gcaggagaag aaagcgactt tgtagttcgg gccctttctt taggttttaa aggtctgtac
     7861 tatcctgact attatgtatt acatccagcg aaagaaagcc atccagtatt tgatgatatt
     7921 ataaaaaata gaatgttatc atatggtggt ggttatggat attttttaca aaaaaatttt
     7981 tatcgtcaag gccggttttt gtctatcaaa aatattttcg gtgtcttttt aaggctaatt
     8041 aaatccttac actcaaaagt tgaatttctt aaatcttttt attttactat tggttttctt
     8101 aagggcttct ttaaaaaatg aaaatagata ttttactatc gacttatgga gaccgtattt
     8161 taggtataaa ccgtctaatt cagtcgattg attttagtaa ttgcaattta ataattgctc
     8221 atcaatttaa aaacaaaata tcatctcaag caaagacctt tattcaaaag cttttgcacg
     8281 aaaataacaa tatatcatat tatcagttaa atagttttgg cgtgactaaa agcagaaatt
     8341 ttttgataaa caaagcaaaa agtaattata tcgcattttg cgatgatgat atatattatt
     8401 tcccgaatgc aatttcaact gtcttagata agatggaaca tgaaaatatt gatgtcgcga
     8461 cttgtatgac tattactgat gatagcaaac ctttgaaaaa ttatgcatca agggactatc
     8521 accataataa aagaacaata ttaagagttg gtacagtaga gataatctgt aaacgtgaaa
     8581 gtattttgaa gactgattta tttccagagg acatgggggc tggtgcttta tttcctctgt
     8641 gtgatgagcc tgttttttta aataattgtt taaaacaatc actcaaggtt cagtttttaa
     8701 gtatacctct ttgttatcat cctaatgttt cttcaggtag taatttaaat agatgctata
     8761 ccattgcaag aggtgtttgt ttcagacgta tatatggcat gcctgcattg ctattgatct
     8821 taatattttc tttgaaaatg aaactgaagt acaggaaatt agacttgcta acttatacca
     8881 aatctcttta tcgggggtat aatgaaggca agtagcgttt tgacaccata tcttaattgg
     8941 ttctatggat ttctgtgctt tttgtcactt tggcctttct tttcatggaa gtggcctgcg
     9001 ataccagtat cctttattgc aatttgcttt tttttatttt tatttgatct ttgttatatt
     9061 aataaattaa aggtgaataa aaagatattc ttaattattt tttcaactct atttgttttg
     9121 attcttttta ttcttcttcc tggaggtatt cctccttggt ttaattatta ttctttgttt
     9181 ttatttctgt tcctattgct acctagaaaa agaatatttg agatatcttt gaagttccgt
     9241 tctatattta ttttttcttt attaccgggg ttggttatct atgcattatt aattatagga
     9301 gttaaactgc catatggtat tcttgatgct cacaatgagc ttaaggattc attgggaatc
     9361 tattatcgtg attatattgg aactgtagct ttgtcgcatt tagttttaac agtaggggac
     9421 tcaactatta tacgattttc tggaatgctt gatgaacctg ggttgttagg aacaatttca
     9481 gcacttctac tgttagcgga taaattaaac tttaaacaca aatcaaacta tgtattgtta
     9541 ttgtcaggag tgataagtat atcacttgct ttttacctat taattttaat ggggcttatc
     9601 tttcaaacac ggcgtaaaat tatttcttta gcaatagtgt tccttattgg catagctata
     9661 taccatagtc ctttttatga aaattttttg caaaaaagaa tttctgacag tgagtcgggg
     9721 cttgttttta gggataatag aatatctgat tgttttaaag aatattacac tgcatttgaa
     9781 aatgatagtg aattaaatct tttttttggt attggtaata atgcgcattt aaggactggc
     9841 tgtgatgtat catcatataa gatgtatata tatgattatg gatatttagg ttttattaca
     9901 atcgctttat tacaaattat gcaatatatg atcccaatta tgggggggta tgcgtgcaaa
     9961 tttttgaagg catcattcgt ttttatttgt ttgttcattc tgtcttacta tcaacgccca
    10021 atttttttta atctagcatt ctccatgatt ttttattact caatttttac ttatttgaaa
    10081 acggtcggca tatctgctac taataaaaaa tagtttttgt aaactatgaa ttaataagtt
    10141 tattactatc cataaccgcc ctgcggtgaa caccccctga caggagtaaa caatgt
//
