LOCUS       AB812041               16390 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: Feb-85.
ACCESSION   AB812041
VERSION     AB812041.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 16390)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..16390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Feb-85"
                     /db_xref="taxon:562"
                     /note="serogroup:O93"
     gene            <1..905
                     /gene="wcaM"
     CDS             <1..905
                     /gene="wcaM"
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01382.1"
                     /translation="YAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILIS
                     DHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVVNITGSDCRQLIHVEN
                     GKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIK
                     GKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNALSFVALTNIEMKRASLELHNKP
                     QHLFMRNINVMQESSVGPALSMNLDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSV
                     DIDRVNHHIVNVEKINFRLPERRE"
     gene            1068..1973
                     /gene="galF"
     CDS             1068..1973
                     /gene="galF"
                     /note="O093_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01383.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     CDS             2319..3080
                     /note="O093_02"
                     /gene="wfdV"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01384.1"
                     /translation="MSTDLVSIITPSYNSEEFIVQTYDSIRNQTYSNWEWIVTDDCSF
                     DRTWEILEEISAADPKVRIYRNKENSGAAVSRNNSIGKSKGKYLAFLDSDDLWEKNKL
                     QEQLGFMKKNGYSFSFTAYMLISENGNKINKKIDARNESVAINYKELLAKKVTLGCST
                     VMLKKDAFEDITMPLIRTGQDYALWLKLLKNNDTKAVLYPQALTMYRIVSGSISRNKV
                     KKALRQWSIYRNLENINLIESLWYFLNYAWRAVFR"
     gene            3091..4290
                     /gene="wzx"
     CDS             3091..4290
                     /gene="wzx"
                     /note="O093_03"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="BAQ01385.1"
                     /translation="MKTSTYSAIAKIISSNISASILTFVSLLILSRVYSVNDYGLFTK
                     YYYLLGIMYVLLDLGLPNALVISLSEKGKSFSIIDVFRKYHKYFIVAIIIVFFVAFYS
                     FSPVIALSFIVCSIVGITLKLNSVKLQIVNDWGGSSRVIFYLPFLRSLGIFGVCFLIV
                     FINEKLDANAIYLVMIVVSIVAMLLSFEKTKNISYAELDGAQDLTKMAWKLYIANIFA
                     IVCMRADVIFISHFFNDAAAGEYARISMIFFAAPLLAGAINTVFIRGLSAQELDIDAV
                     KNITHKLFLFTVIGLLLLLAVAYLSKAIIMEYCSANDIYIFVILTIAYAGTLVFGVWE
                     AFINHRDQSFFLILKVSQLIFFIVTFLICYKTVGFIIGAIAFLASRLGGWILQWIYCN
                     RRKYLFT"
     gene            4312..5823
                     /gene="wzy"
     CDS             4312..5823
                     /gene="wzy"
                     /note="O093_04"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="BAQ01386.1"
                     /translation="MTPDVYFSHHKEEQSNKISYGVLIVLVALLSGYYVQYLSDTIYG
                     VIISYLMLLAVVISSFKGLDSAVKIYLLFSLITPQFSRTFVEQVVNSNNQEVGSFYSF
                     HSVQLWGLGLSVVAIIYIGLIALCKLFYTHAKLQRTNVSSYFISLYVVCGFLLLATII
                     STAIGKVPDMRNIISDARLFIVWTIGALVANLIYKDKNIIVKIYDILFFSICVVGVRT
                     LYFLLNDYVTGTPNLEYATQAYVAYPLMFVFAMATRNKLKRAVLTGLSLLAAISMKRE
                     DIAFVFICIMLLLFLILLFRNNILRTNAIIALVMLILIAFLFVFTLSVYSPQSFDFLL
                     YKFDFFSKLITGSEQSSGSVLVRLYEIRNIIGASINYIYPIFVGFGFGGYFDFSLTGF
                     PINLGISDYSFNEILQNKFVRPHSFTNYIFLKGGLIFLIYYLGVILLFMNQSYKMMKK
                     TLIIGTIYSKEFKVYLFCFLYAVFCVNMFWQPMHIFLFAFLLNLLLLKNIKRN"
     CDS             5830..6834
                     /note="O093_05"
                     /gene="orf5"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ01387.1"
                     /translation="MKILIADPLSEKGHLNFNLGVIEWFKNDQHITIDYYVSEYMQCA
                     MQLVCKVFPDRDLDKTNIIKYKIKQLKILNDIISYANNHGYDKLIFLSYEVYSTAVYM
                     NFMRLIRKNGLEIYLIEHNTIPTSFLKNIFYSIIPKKNITHIGLVTYISEYIKNKYKK
                     NSLYIPHPMRKLPVREYRKEKTIKVFMPSFVEQKLKNEICDSFRKLGEGYLLCTKGMS
                     GVLDNIIFKEYYDNYDEELTIADAIFIPQYFDYRVSGVFYEALQTHAKIFMSNCIFSQ
                     YMKTIFKDKIIIIDSWEEYICNHFVSEMNIPQVKKDVNENYFNHEGLKIVRENILCLE
                     "
     CDS             6822..7364
                     /note="O093_06"
                     /gene="wexC"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="BAQ01388.1"
                     /translation="MFRMKKNLMYIYVGIWNHMICYIPSYYLRWFFLRFFYRAKIDKG
                     VNIHMGVKFFSPWKLKVKSGTNIQWGSFLDCRGGLDIGHNVDITLGVKILTQYHNIHD
                     ERYETISKKIIIEDNCIIGSYSLLLPGSNIEKFGVLGAGSVLTKNVKENTMFAGNPAK
                     YIKRRNLHNIINVSYKRAFH"
     CDS             7366..8454
                     /note="O093_07"
                     /gene="wfdW"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="BAQ01389.1"
                     /translation="MNKIAIIDPASYALPYDYFFIRSLSKDYEIDFFCSKTKYNFDYV
                     KKISLIPNVKVHVYNVSYGSKLLGVISYILMLLKVVFTSKKYTAINLQWSLLPYVEVI
                     FLYIIKSKFVMTFHNSVEHGRIKKAYLKNNILASLANKIIFVSDFTKNEFLSIYNKLN
                     NISEKSYVLKHGVMPINFDEANIKNNFFEAPRKIIFWGNVKDYKGVDFLAENIHLIIA
                     KGYSLEIYGKFDRDKKYLINMFQRAGAKVTDEYLSLNEIEKLLSSNIVLVLPYKAASQ
                     SGVMYTALNYNTVMITTDCGDPYEFLKDCDLRNLSFTYNDSASFEKALDYLSNNFLSI
                     KERILLNKKKYQWEYSSTILKEIFGSKI"
     gene            8451..9860
                     /gene="manC"
     CDS             8451..9860
                     /gene="manC"
                     /note="O093_08"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="BAQ01390.1"
                     /translation="MNVNNCAKIVPVIIAGGVGSRLWPMSREEHPKQFLPLLHDSLSL
                     LQQTLSRVNNKHFDAPLVICNEEHRFLVAQQLKELNLLNNNIILEPCSRNTAPAITLA
                     ALSLKNKDCIMLVLAADHFIGDGDYFLNFIKKASTAIDDQSLITFGITPSHPETGYGY
                     IKKGKSISDDVFMVDKFVEKPNKEKAEEYIAEKLYLWNSGMFMFKPMAYLRELKKYHP
                     NIYDVCSSSIDSIVSDMDFRRIDKTIFESCANESVDYAVMENSSHVKVLQFDSQWSDV
                     GAWGALWEISDKDTHGNCISGDVFLNDSKNCYIKSDGVFTSAIGLNNIVVVASKDSVL
                     VSHRDSVQNVKNVVNYLKENNRYEYKRHNVRYLPWGTTLSLVDSSKYKINFVTIEPGK
                     SISLQKHYHRNEQWTILSGTAVVYIDGIKKIITENQTVHIPIGCTHSIHNPGIIPVEF
                     LETQIGECIDPSDVFRLEK"
     gene            9872..11242
                     /gene="manB"
     CDS             9872..11242
                     /gene="manB"
                     /note="O093_09"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="BAQ01391.1"
                     /translation="MNCLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQITLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLSK"
     gene            11406..12812
                     /gene="gnd"
     CDS             11406..12812
                     /gene="gnd"
                     /note="O093_10"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01392.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKDGVFHTEWLD"
     gene            13061..14227
                     /gene="ugd"
     CDS             13061..14227
                     /gene="ugd"
                     /note="O093_11"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01393.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            14317..15351
                     /gene="wzz"
     CDS             14317..15351
                     /gene="wzz"
                     /note="O093_12"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01394.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGS
                     AAPKVSEIQSMLIGRFSTTFSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(15449..16060)
                     /gene="hisI"
     CDS             complement(15449..16060)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01395.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT     4927 a   2991 c   3545 g   4927 t
ORIGIN
        1 attacgccat cttacgccag ggatttcata accagattat cggtgccaac atcaccaatt
       61 gtaagttcag cgacttacag ggggacgcta ttgaatggaa cgtggcgatt aacgacagtg
      121 atattttgat atctgaccat gttatcgagc gcatcaactg taccaacggc aaaatcaact
      181 ggggcatcgg cataggtctt gcgggaagta cctacgataa caactacccg gaagaccagg
      241 cagtgaaaaa ctttgtcgtg gtgaatatca cgggatcgga ttgtcggcag ttgatccatg
      301 ttgaaaatgg taaacatttt gttattcgta atatcaaagc ccgcaatatc acgccggatt
      361 tcagtaagaa agcaggtatt gataacgcca cagtcgctat ttacggttgt gacaatttcg
      421 tgattgataa tattgaaatg attaatagcg ccgggatgtt aatcggctat ggggtaatta
      481 aaggcaaata tctctcgata ccgcaaaatt tccgagtgaa taatattcaa ctggataaca
      541 ctcaccttgc ttataaattg cgcggcatcc aaatctccgc cgggaatgct ctctcctttg
      601 tggcgctgac taacattgag atgaagcgtg cgtcgctgga gttacacaac aaaccgcaac
      661 atctttttat gcgaaatatc aatgtgatgc aggaatcctc tgttggaccc gcattgagca
      721 tgaacttgga catgcgcaaa gacgttcgcg gcgtctttat ggcgaaaaaa gaaacactgc
      781 tgtctcttgc aaatgttcat gcggtgaatg aaagagggca aagctccgtc gatatcgaca
      841 gggttaatca ccatattgtt aatgtggaaa agattaactt tagattgccg gaacggaggg
      901 agtagatttg cgaccattcc tggaaaaatg gagccatact taggaacaat gctactgcaa
      961 tccacaacga agcggcgtaa catcacaagt aattcagtaa tcaattcagg gtaattgatg
     1021 ctggcgaaaa aaatcgaaca agctataatt cagcaaccat tttacaggtg gatgaaataa
     1081 tgacgaattt aaaagcagtt attcctgtag cgggtcttgg gatgcatatg ttgcctgcca
     1141 ctaaggcgat acccaaagag atgctaccga tcgtcgacaa gccaatgatt cagtacattg
     1201 ttgacgagat tgtggctgca gggatcaaag aaatcctcct ggtaactcat gcgtccaaga
     1261 acgcggtcga aaaccacttc gacacctctt atgaattaga atctctcctt gaacagcgcg
     1321 taaagcgtca actgcttgcg gaagtgcagt ccatctgtcc accgggcgtg accattatga
     1381 acgtgcgtca gggcgaacct ttaggtttgg gtcactccat tttatgtgca cgacccgcca
     1441 ttggtgacaa cccatttgtc gtggtgctgc cagacgttgt tatcgatgac gccagcgccg
     1501 acccgctgcg ctacaacctt gcagccatga ttgcgcgctt caacgaaacg ggccgtagcc
     1561 aggtgctggc aaaacgtatg ccgggtgacc tctctgaata ctccgtcatt cagaccaaag
     1621 agccgctgga ccgcgaaggt aaagtcagcc gcattgttga atttatcgaa aaaccggatc
     1681 agccgcagac gctggactca gacatcatgg ccgttggtcg ctatgtgctt tctgcagata
     1741 tttggccgga acttgaacgc actcagcctg gtgcatgggg gcgtattcag ctgactgatg
     1801 ccatcgctga actggcgaaa aaacagtccg ttgatgccat gctgatgaca ggtgacagct
     1861 acgactgcgg taaaaaaatg ggttatatgc aggcatttgt gaagtatgga ctacgcaacc
     1921 tgaaagaagg ggcgaagttc cgtaaaggga ttgagaagct gttaagcgaa taatgaaatc
     1981 tgaccggatg taacggttga taagaaaatt ataacggcag tgaagattcg tggcgaaagt
     2041 aatttgttgc gaatattcct gccgttgttt tatataaaca atcagaataa caacgagtta
     2101 gcaataggat tttagtcaaa gttttccagg attttccttg tttccagagc ggattggtaa
     2161 gacaattagc gtttgaattt ttcgggttaa gcgcgagtgg gtaacgctcg tcacatcgta
     2221 ggcatgcatg cagtgctctg gtagctgtaa agccaggggc ggtagcgttt ctatgaattt
     2281 cttttgaaca ataaattaag aggaaataga ttaacacgat gagcactgat ttagtttcta
     2341 ttattacgcc atcatataat tcagaagagt ttatcgttca gacttacgat tcgatacgta
     2401 atcaaactta cagtaattgg gaatggattg ttactgatga ttgttcgttt gatagaactt
     2461 gggaaatcct tgaagagata agcgcagcgg atccaaaagt aagaatttat aggaataaag
     2521 agaatagtgg tgctgcagta tcgaggaata acagcattgg aaaatctaag gggaaatatt
     2581 tagctttcct ggatagtgat gacttatggg agaaaaataa actgcaagaa caactgggat
     2641 ttatgaaaaa aaatggttat agtttcagtt ttacagcgta tatgcttatt tcagaaaatg
     2701 gcaataaaat taataaaaaa atagatgcaa gaaatgaatc agtagcaatt aattataaag
     2761 aacttcttgc taaaaaggtt acattaggtt gctctacagt catgttgaaa aaagatgctt
     2821 ttgaagatat aacaatgcca ttgatacgta cagggcaaga ttacgcactt tggttaaagt
     2881 tgctgaaaaa taatgatact aaggcggttt tatatccgca agcattaaca atgtatcgta
     2941 tcgtttcagg ttctatttca aggaataaag taaaaaaagc tctgaggcaa tggtctattt
     3001 atcgtaatct agaaaatatt aatttaatag aatcactgtg gtatttttta aattatgcat
     3061 ggcgagccgt ttttcgatga agaggattat gtgaagacaa gtacatattc tgcaattgcg
     3121 aagatcattt caagtaatat atctgcatca atcttaactt ttgtatcttt actgatatta
     3181 agtcgagtat atagtgtaaa tgattatggt ctatttacca aatattatta tttgcttgga
     3241 ataatgtatg ttttattgga cttggggcta ccaaatgcat tagtgatttc actttcagaa
     3301 aaagggaaat cttttagcat aattgatgta tttagaaaat atcataagta ttttattgta
     3361 gcgataataa tagttttttt tgttgcattt tactcatttt caccggtgat tgcattatca
     3421 tttatagtat gtagcattgt cggtattact ttaaaattaa actctgtaaa actgcaaatt
     3481 gttaatgatt ggggaggaag ttcaagggtt attttttatc ttccttttct acgaagtcta
     3541 gggatatttg gggtctgttt tctaatagtt ttcatcaatg agaagcttga cgcaaatgct
     3601 atatacttag taatgatagt tgtgagtata gttgctatgt tgctaagttt tgagaaaact
     3661 aaaaatataa gttatgcaga gttggatggt gcacaagatc ttacgaaaat ggcatggaaa
     3721 ttgtatattg cgaatatatt cgcaatagta tgtatgcgtg ctgatgtcat ttttatatcg
     3781 cattttttta atgatgcggc ggcaggagaa tatgcaagga tatcaatgat attttttgca
     3841 gcacctttgc ttgcaggagc tattaatacg gtattcatta gaggactgtc tgcacaagaa
     3901 cttgatattg atgcagtaaa aaatatcacg cataaattat ttttatttac agtgataggc
     3961 ctattattgc ttttagcagt agcttacctt agtaaagcta ttattatgga atattgtagt
     4021 gctaacgata tttacatatt tgttatttta actatagcat atgcgggaac tctggttttt
     4081 ggtgtttggg aggcatttat aaatcataga gatcaatctt tcttcctgat tctaaaagta
     4141 tcgcaactta tatttttcat tgttacattt ttaatatgct ataaaacggt cggttttatt
     4201 attggtgcga ttgcatttct tgcatctaga ttaggtggct ggatattgca atggatttat
     4261 tgtaaccgac gaaaatatct ttttacttaa tatttcttat ggagatataa attgactcca
     4321 gatgtatatt tttcacatca caaagaagaa caatcaaata aaatttctta tggggttctg
     4381 attgtcttag ttgcattgtt atccggttac tatgtgcaat atttatcgga tacaatttac
     4441 ggtgttatta tttcttattt aatgctttta gctgtagtta tttcaagctt taaaggttta
     4501 gattctgctg taaaaatata tttgttattt tcgttaatta cacctcaatt ttcgagaaca
     4561 tttgttgaac aagtagtgaa tagcaataat caagaggttg gatcttttta tagctttcac
     4621 tctgtacaat tatgggggtt aggtttatca gtagttgcaa ttatatatat aggtttaatt
     4681 gctctatgta agttgtttta tactcacgct aagctacaaa gaacgaatgt ctcatcttat
     4741 ttcataagtt tatatgtggt ttgtggattt ctattattgg ctacgataat ttccactgct
     4801 atcggcaaag tgcccgatat gcgaaatatt atatcagatg cccgtttatt tattgtatgg
     4861 actattggtg ctttagtcgc gaatttaatt tataaagata agaatataat agtaaaaatt
     4921 tatgatattc tctttttttc aatatgtgtg gttggggtaa gaactttata ttttctgcta
     4981 aatgattacg tgacgggtac tccaaattta gaatacgcaa ctcaagctta tgtggcgtac
     5041 ccattaatgt ttgtttttgc tatggcaacg cgtaataaat tgaaaagagc agtacttact
     5101 ggtctttcat tgttggcagc aatatcaatg aaacgtgaag atatagcatt tgtatttatt
     5161 tgcataatgc tgttgctttt tcttattctt ctatttagaa ataatattct tcgtacaaat
     5221 gctattattg cgctagtaat gttaatactt attgcatttt tattcgtatt cacattgtca
     5281 gtatattcgc cacagagctt cgatttttta ttatataaat ttgatttctt ctcaaaatta
     5341 attacaggta gtgaacaatc atctggttca gttttagtta ggctatatga gattcgaaat
     5401 atcattgggg caagtataaa ctatatctat cctatttttg tggggtttgg attcggtggc
     5461 tattttgatt tttctctcac aggatttcca ataaatttag gtatttcaga ttacagtttt
     5521 aatgaaatct tgcaaaataa atttgtgcgc cctcactcat ttactaatta tatatttctt
     5581 aaaggtggat tgattttttt aatttattat ttaggcgtga ttttgttatt catgaaccaa
     5641 tcatacaaaa tgatgaaaaa aactctgatt ataggcacta tttatagcaa agaatttaag
     5701 gtttatttgt tttgtttttt gtatgctgta ttttgtgtaa atatgttttg gcaaccaatg
     5761 catatttttc tattcgcatt tttattaaat ttattattgc tgaaaaatat aaaaaggaac
     5821 tagtatataa tgaaaatatt aatagcagat cctctatcag aaaaagggca tttgaatttc
     5881 aatttgggag taatagagtg gtttaaaaac gaccagcata taactattga ttactacgtt
     5941 tctgaatata tgcaatgtgc tatgcaactg gtttgtaagg ttttcccaga tcgtgaccta
     6001 gacaaaacca atataataaa atataaaatt aagcagctga aaatattgaa tgatattatt
     6061 tcttatgcaa ataatcatgg ctatgataaa ttaatatttc tatcgtatga agtttattct
     6121 acagctgttt atatgaattt tatgagactc attagaaaga atggtcttga aatatatttg
     6181 atagaacata atactatacc gacaagtttc ttaaaaaata tattctatag tataataccc
     6241 aaaaaaaata tcacacatat tggacttgtg acttatataa gtgaatacat taaaaacaaa
     6301 tataaaaaaa atagtttata tattcctcat ccaatgagaa aacttccagt tagagagtat
     6361 cgaaaagaaa agactattaa agtatttatg ccttcttttg ttgagcaaaa attaaagaat
     6421 gaaatatgcg atagttttcg caaattagga gaaggttatt tgttatgtac taaaggaatg
     6481 tcaggggtat tagataatat catatttaaa gaatattacg ataattatga tgaagaactt
     6541 acaattgcag atgccatttt cattccacaa tatttcgact atagagtaag tggagttttt
     6601 tacgaagctc tgcaaaccca cgcaaaaata ttcatgagta attgcatctt cagccaatat
     6661 atgaaaacta tattcaagga taaaattatt ataatcgatt cgtgggagga atatatatgc
     6721 aatcattttg ttagtgaaat gaatattcca caagttaaaa aagatgtcaa tgaaaattat
     6781 ttcaaccacg aaggattaaa aatagttaga gagaatattt tatgtttaga atgaaaaaaa
     6841 acttaatgta tatatatgtt ggtatatgga atcatatgat ttgctatatt ccttcatatt
     6901 atctgagatg gtttttttta cgtttttttt acagggcaaa gattgacaaa ggtgtcaata
     6961 ttcatatggg ggtaaagttt tttagtcctt ggaaattaaa ggttaaatca ggtacaaata
     7021 tacaatgggg gtcattccta gattgccgag gaggcctaga tataggtcat aatgttgaca
     7081 taacattagg ggtgaaaata cttacacaat atcataatat tcacgatgaa agatatgaaa
     7141 cgatctcaaa gaaaataatt atagaagaca attgtattat aggatcatac tcactattat
     7201 taccaggaag taatattgaa aaatttggtg ttttaggcgc tggttctgtc ctaacaaaaa
     7261 atgttaaaga aaatacgatg tttgcaggta atccggcaaa atacattaaa agaagaaatc
     7321 ttcataatat tataaatgtc agttataaaa gagcgttcca ttaatatgaa taaaatagct
     7381 ataatagatc ctgcatcgta cgcattacca tatgactatt tttttattag aagtttgtca
     7441 aaggattatg agattgattt tttttgttcg aaaacaaaat ataattttga ttatgttaag
     7501 aagatttcat taattcctaa tgtcaaagtt catgtctaca atgtatctta tgggagtaaa
     7561 ttgttggggg tgattagtta tatattaatg ttattaaagg ttgtgtttac ttcgaaaaaa
     7621 tatactgcca ttaatttaca atggtcattg ttaccgtatg ttgaagttat ttttctctac
     7681 attattaaat ctaaatttgt tatgactttc cataatagtg ttgagcatgg gcggataaaa
     7741 aaagcatatc taaaaaataa catcctcgct tctctcgcta ataaaatcat attcgttagt
     7801 gatttcacaa aaaatgaatt tttatcaata tacaacaagt taaataatat tagtgagaaa
     7861 tcatatgtgt taaaacatgg tgtgatgcca attaacttcg atgaagctaa tattaaaaat
     7921 aacttttttg aagcacctag aaaaatcatt ttttggggca atgtgaaaga ctataaagga
     7981 gttgattttc tcgctgaaaa catacattta ataatagcta aaggttacag tctggagata
     8041 tatggaaaat ttgatagaga taagaaatat cttataaata tgttccagcg ggctggtgcg
     8101 aaagtgaccg atgaatattt atcattaaat gaaattgaaa aattattatc ctccaatata
     8161 gttcttgtac taccatacaa agccgcttca caatcaggtg ttatgtatac agctctaaat
     8221 tataatacag tgatgataac aacagattgt ggagatccat atgaatttct gaaagactgt
     8281 gacttgagaa atttatcatt tacttataat gattcagcta gctttgaaaa agccctagat
     8341 tatttatcta ataatttcct ttcaattaaa gagaggattt tgctgaataa gaaaaaatat
     8401 caatgggaat attcatctac tatacttaaa gagatttttg gtagcaaaat atgaatgtaa
     8461 ataattgtgc taaaatcgtt cctgtaatta ttgctggtgg tgtgggtagt cggctttggc
     8521 ctatgtcacg agaggagcat ccaaagcagt ttttacctct tttacatgac tcattatcac
     8581 ttctccaaca gacgttatct agagtgaata ataagcattt tgacgcaccg ttagttattt
     8641 gtaatgaaga acatcgtttt ttagttgcgc agcaattaaa agaattgaat ttattgaata
     8701 acaatataat tcttgaaccc tgtagcagaa acaccgcacc tgcgataaca cttgcagcac
     8761 tatccttgaa aaataaagat tgtattatgc ttgttttagc tgctgatcat tttattggag
     8821 atggtgatta ttttttaaat tttattaaaa aagcatcaac tgctattgat gatcaaagcc
     8881 tcataacgtt tgggatcacg ccatcccatc cggaaacagg gtatggatat attaaaaaag
     8941 gaaaatcaat atcagatgat gtattcatgg ttgataagtt tgtagaaaaa ccaaataaag
     9001 aaaaagcaga ggaatatata gctgaaaaac tatatttatg gaacagtgga atgttcatgt
     9061 ttaaaccaat ggcatactta agagaactaa aaaaatacca tcctaatata tatgatgtat
     9121 gttcgagttc gatagattca attgttagtg atatggattt tagaagaatt gacaagacta
     9181 ttttcgaaag ctgtgctaat gaatctgttg attacgcagt catggaaaat agttctcatg
     9241 tcaaagtttt gcaatttgat tctcaatgga gtgatgtagg agcttgggga gcattatggg
     9301 aaatctcgga taaagatact catggtaatt gtatttcagg tgatgttttc ttaaatgatt
     9361 caaaaaattg ctatattaaa agtgatggtg tttttacttc tgctatcgga ttaaataata
     9421 tagttgttgt cgcgtccaaa gattcagtat tagtctcaca tcgggactct gttcaaaatg
     9481 ttaaaaatgt cgttaattat ttaaaggaga ataatcgata tgaatacaag cgacataacg
     9541 ttcgttacct gccttgggga acaactttat ctttagtcga ttcttctaaa tataaaatta
     9601 actttgttac tattgagcca gggaagtcaa tctcattaca aaaacactat catcgtaacg
     9661 aacaatggac gatactatct gggactgcag tggtatatat agatggaata aaaaaaataa
     9721 tcacagaaaa tcaaactgtc catataccta tcggttgtac gcactctatt cataatcctg
     9781 gtataattcc ggttgaattt ttagaaactc aaatcggaga gtgtattgat ccgagtgatg
     9841 tatttagact tgaaaaatga aggtataagg aatgaattgt ttaacctgct ttaaagccta
     9901 tgatattcgc gggaaattag gcgaagaact gaatgaagat atcgcctggc gtattgggcg
     9961 cgcttatggc gaatttctca aaccgaaaac cattgtgtta ggcggtgatg tccgtctcac
    10021 cagcgaaacc ttaaaactgg cgctggcaaa aggtttacag gatgcgggcg ttgacgtgct
    10081 ggatatcggt atgtccggca ccgaagagat ctatttcgcc acgttccatc tcggcgtgga
    10141 tggcggcatt gaagtcaccg ccagccataa cccgatggat tacaacggca tgaagctggt
    10201 gcgcgaaggg gctcgcccga tcagcggtga taccggactg cgcgacgtcc agcgtctggc
    10261 agaagccaac gactttcctc ccgttgatga aaccaaacgc ggtcgttatc agcaaattac
    10321 cctgcgtgac gcttacgttg atcacctgtt cggttatatc aacgtcaaaa acctcacgcc
    10381 gctcaagctg gtgattaact ccgggaatgg cgcagcgggt ccggtggtgg acgccatcga
    10441 agcccgcttt aaagccctcg gcgcacctgt ggaattaatc aaagtgcaca acacgcccga
    10501 cgggaatttc cccaacggta ttcctaaccc gctgctgcca gaatgccgcg acgacacccg
    10561 caatgcggtc atcaaacacg gcgcggatat gggcattgcc tttgatggcg attttgaccg
    10621 ctgtttcctg tttgacgaaa aagggcagtt tatcgagggc tattacattg tcggcctgct
    10681 ggcagaagca ttcctcgaaa aaaatcccgg cgcgaagatc atccacgatc cacgtctctc
    10741 ctggaacacc gttgatgtgg tgactgccgc aggtggcacc ccggtaatgt cgaaaaccgg
    10801 acacgccttt attaaagaac gtatgcgcaa ggaagacgcc atctacggtg gtgaaatgag
    10861 cgcccaccat tacttccgtg atttcgctta ctgcgacagc ggcatgatcc cgtggctgct
    10921 ggtcgccgaa ctggtgtgtc tgaaaggaaa aacgctgggc gaactggtgc gtgaccggat
    10981 ggcggcgttt ccggcaagcg gtgagatcaa cagcaaactg gcgcaacccg ttgaggcgat
    11041 taatcgcgtc gaacagcatt ttagccgtga ggcgctggcg gtggatcgca ccgatggcat
    11101 cagcatgacc tttgccgact ggcgctttaa cctgcgcacc tccaacaccg aaccggtggt
    11161 acggttgaat gtggaatcgc gcggtgatgt gccgctgatg gaagaaaaaa caaaacttat
    11221 ccttgagtta ctgagtaagt aatccagtaa tttcatataa atgggtttta aaagacggaa
    11281 aagatgagat atccagtgtg gtatagccaa ggtaatgcta ttcagcatct ctatgagtga
    11341 gttaacatct ataccacatt taagccgcac actcggcggt gaccaccccc tgacaggagt
    11401 aaacaatgtc aaagcaacag atcggcgtcg tcggtatggc agtgatgggg cgcaaccttg
    11461 cgctcaacat cgaaagccgt ggttataccg tctctatttt caaccgttcc cgtgagaaaa
    11521 cggaagaagt gattgccgaa aatccaggca agaaactggt tccttactat acggtgaaag
    11581 agtttgttga atctctggaa acgcctcgtc gcatcctgtt aatggtgaaa gcaggtgcag
    11641 gcacggatgc tgctattgat tccctcaagc catacctcga taaaggtgat atcatcattg
    11701 atggtggtaa caccttcttc caggacacca ttcgtcgtaa ccgtgagctt tctgcagaag
    11761 gctttaactt tatcggtacc ggtgtttccg gtggtgaaga aggtgcgctg aaaggtcctt
    11821 ccattatgcc tggcggacag aaagaagcct atgaactggt tgcgccgatc ctgaccaaaa
    11881 tcgccgcagt ggctgaagac ggcgaaccgt gcgttaccta tattggtgcc gatggtgcag
    11941 gtcattatgt gaagatggtt cacaacggta ttgaatacgg tgatatgcag ttgattgccg
    12001 aagcctattc tctgctaaaa ggtggcctga accttaccaa cgaagaactg gcacagacct
    12061 ttaccgaatg gaataacggt gaactgagca gctacctgat cgacatcacc aaagatatct
    12121 tcaccaaaaa agatgaagac ggtaactacc tggttgatgt gattctggat gaagcagcaa
    12181 ataaaggtac gggcaaatgg accagccaga gtgcgctgga tctcggcgaa ccgctgtcgc
    12241 tgattaccga gtctgtgttt gcacgttata tctcttctct gaaagatcag cgcgttgccg
    12301 cgtctaaagt actgtctggt ccgcaagcgc agccagcagg tgacaaggct gagttcatcg
    12361 aaaaagtacg ccgtgcgctg tatctgggca aaattgtttc ttatgctcag ggcttttctc
    12421 agctgcgtgc tgcgtctgaa gagtacaact gggatctgaa ctacggtgaa atcgcgaaga
    12481 ttttccgtgc tggctgcatc atccgtgcgc agttcctgca gaaaatcacc gatgcatatg
    12541 ccgaaaatcc gcagatcgct aacctgctgc tggctccgta cttcaagcaa atcgccgatg
    12601 actatcagca ggcgctgcgc gatgtcgtcg cttacgcagt acagaacggt atcccggttc
    12661 cgaccttcgc tgctgcggtt gcctattacg atagctaccg tgccgctgtt ctgcctgcga
    12721 acctgatcca ggcacagcgt gactatttcg gtgcgcatac ttataagcgc attgataaag
    12781 acggtgtgtt ccataccgaa tggctggatt aatctgattt aaatcaatta atcaatgcaa
    12841 ggcccggaga aaccctccgg gcttttttat tatacaaagc ggcaggttat ggcctttttt
    12901 tataatttat agttaaaaac gcgatataat acagcgccgc acagcaggat cgctgccttg
    12961 acagttcatc tacatcagcg ttaaaaatcc cgcagtagat gaagttgtgg tggtggatta
    13021 atgaccactc taaatgttta accggaagaa gtcagagcta atgaaaataa caatttcagg
    13081 aacaggttat gttggtcttt caaatggtat tctgattgcg caaaatcacg aagtggttgc
    13141 actggatatc gttcaggcca aagtggacat gcttaacaag aagcagtcac cgattgttga
    13201 taaggagatt gaagagtatc tggcgactaa agatctcaat ttccgcgcta cgacagataa
    13261 gtatgacgcg tataaaaatg ccgattacgt tattattgcc acgcctaccg attatgatcc
    13321 gaaaacaaat tattttaata cctcaagcgt cgaagcggtc atccgtgatg tgacagaaat
    13381 taatcccaac gcggtaatgg tgattaaatc tacgattccg gtaggtttca ccaaatcaat
    13441 taaagaacgt ctgggtatcg ataaccttat tttctctccg gaatttctcc gtgaaggtaa
    13501 agccctttac gataatctcc atccttcacg tattgtcatt ggtgagcgtt cagaacgcgc
    13561 agaacgtttt gctgcgttgt tacaggaagg cgcgattaag caaaatatcc ctacactatt
    13621 taccgactcc actgaagcag aagcgattaa acttttcgct aatacctatc tggcgatgcg
    13681 cgtagcatac tttaatgaac tggatagcta tgcagaaagt ttaggtctga atactcgcca
    13741 gattatcgaa ggcgtttgtc tcgatccgcg tattggcaac cattacaaca acccgtcgtt
    13801 tggctatggt ggttattgtc tgccgaaaga taccaagcag ttactggcga actaccagtc
    13861 tgtgccgaat aacctgattt cggcaattgt cgatgcaaac cgcacgcgta aagactttat
    13921 tgccgatgcc attttgtcac gcaagccgca agtggtgggt atttatcgtc tgattatgaa
    13981 gagcggttca gataacttcc gcgcgtcttc cattcagggg attatgaagc gtatcaaggc
    14041 gaaaggcgtt gaagtgatca tatacgagcc agtgatgaaa gaagactcat tcttcaactc
    14101 tcgcctggaa cgagatctcg ccaccttcaa acaacaagcc gacgtcatta tttccaaccg
    14161 tatggcagaa gagcttaagg atgtggcaga caaagtctac acccgcgatc tctttggcag
    14221 tgactaacat cctgttatca gggcgatttt cgccctgatt ctcttatgtt ccctttgtaa
    14281 taattcatta tttttatcat ttatcctata gcattcatgg cgattatcgc taaactatgg
    14341 cgacttaaaa ttatttcgtc cgttagggta atgatgagag tagaaaataa taatgtttct
    14401 gggcaaaacc atgacccgga acagattgat ttgattgatt tactggtgca gttgtggcgc
    14461 ggcaagatga caattatcat ctctgtcatt gtggctattg ccctggctat agggtatctg
    14521 gctgttgcca aagaaaagtg gacttccaca gctatcgtta ctcaacctga tgtggggcaa
    14581 atcgctggtt ataacaatgc cataaatgtg atttatggct cggcagcgcc gaaagtgtct
    14641 gagatccagt cgatgttaat tggtcgtttc agcacgacat tttctgcatt agcagaaacg
    14701 ctggataatc aggaagaagc agagaagctc accattgagc ctaccgtcaa aaatcagtca
    14761 ttacctttag cggtgtctta tgttggtgaa acacctgaag gtgcgcagaa acagctggcg
    14821 aagtatatcc agcaagttga cgaccaggta aatgaagaac tggaaaaaga cctgaaagac
    14881 aacatcgctc tgcgtatgaa aaacttgcag gattctctga aaactcagga agtagttgcg
    14941 caggagcaga aagatctgcg tatccgtcag attcaggaag cgttgcagta tgcgaatcag
    15001 gcgcaggtga caaagccgca gattcagcag acacaggacg taacccaaga tactatgttc
    15061 cttttgggga gcgaagcgct ggagtcgatg attaagcacg aggcgactcg tccgttggtg
    15121 ttctcatcaa actactatca gactcgtcaa aacctgctgg atatcgacaa tcttgacgtt
    15181 gataaacttg atattcatgc ttaccgctat gtaatgaaac cgacgttacc tattcgtcgc
    15241 gatagcccga aaaaggcaat taccttgatt ctggcggtgc tgctgggcgg catggttggc
    15301 gcggggattg tgttggggcg taacgctctg cgtaattaca acgcgaagta atcttttcgg
    15361 ttttaaagaa aatgggcagg gtggtgacac cttgcccgtt tttttttgcc ggatgcgaca
    15421 acaatatcgc atccgcttac cccgcaactc actgatgccg tttacgcagg ttctcaatca
    15481 ccgtcgttaa atccagatcc tgatcctgca acaataccaa caggtgatac atcaaatcag
    15541 acgcctcgtt ggtcagctca aagcggtcat gtacagtcgc ggccagtgcg gtttccacgc
    15601 cttcttcgcc cactttctgc gcaatgcgtt tggtgccgct ggcatacagt ttggcggtgt
    15661 aggaggtttc cgggtcggca gatttacgtt cggcgagcag ttgttccagt tgatacagga
    15721 acagccactg gtgagcggtg tcgccgaagc agctgctggt gcctttgtgg caggttggcc
    15781 cgatgggatt cgccagcact aataaagtgt cgttgtcgca gtccggcgca atatttacta
    15841 cgttgaggaa attgcccgat gtttcgcctt tggtccacag tcgctgttta gtgcgcgaga
    15901 agaaggtgac tttgccgctt tcgatggttt tgtctaaggc ttccgggttc atatagccca
    15961 gcattaacac ttcgccggat accgcgtgtt gcacaatcac cggcatcagt ccgtcggttt
    16021 tttcccagtc cagttcgcga cgttgttgtt ctgttaacat atcctgatct ccacgccctg
    16081 tgttgccagg tacgctttta attcaccaat attgattatt tgtttgtgga atacggaagc
    16141 tgccagcgcg ccgtcaacgt cggcatcacg gaaggcttcg aggaagtgtt ccatggtgcc
    16201 cgcgccaccg gaggcaatta gcgggacgtg gcaaacttca cgtacttttt tcagctgctc
    16261 gaggtcgtaa ccgttacgca cgccgtcctg attcatcatg ttaaggacga tttctccggc
    16321 accgcgtttt tgcacttcct gtacccagtc gagcgtttcc cactgagtga cgcgggtgcg
    16381 gctttcatcg
//
