LOCUS       KJ778792               11442 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain 6941-60 serotype OX10:H? O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778792
VERSION     KJ778792.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11442)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11442)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="6941-60"
                     /serotype="OX10:H?"
                     /db_xref="taxon:562"
     misc_feature    1..11442
                     /note="O-antigen gene cluster"
     gene            7..1386
                     /gene="wzx"
     CDS             7..1386
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AJE24480.1"
                     /translation="MQCSGSCKARGGSVENIENVCMVIKSSNGFGRIRINILLGFFSK
                     FGSFILSYISLPIALSYLGVNYYGVWIVIFSVVAWIYNFDIGIGLGLKNSLNDALVKK
                     NYKLANELIATAYIFLIAVSILLLLISFLLVFSINLDSIFSMSNIRESEIRTTLLVSV
                     FFTIANFILLLYKQLFFAVNLSGFAAVTTILYQVLVIVFLLVAKCFIEPSLLIVAIIY
                     GVSNLFVGVFFTLYFFKRNDVLKFSFSNYKRNHIKSIGGVGIKFFIIQMCMLMIFTCD
                     NIIISKYLGPEYVTSYSLILKTYQAFIMISYIILTPYWTLFAEAYYQKNTEWILKQYK
                     KLYTLFLLMIIAVIAYSLYIQPLLKIWLHTELKFNKYMIYGFCIFVIIRVFIDLHAVF
                     LNGIGKINVQLVLYLFAAIINIPLAIILIKHFNMGSEAVIFSASIAIAPMAIILPLQS
                     LVLFRNIAK"
     CDS             1383..2120
                     /codon_start=1
                     /transl_table=11
                     /gene="orf2"
                     /product="glycosyltransferase"
                     /protein_id="AIG62673.1"
                     /translation="MKLTVITVVYNSIEMFKKTYFCLSQQTCQNFTWIIKDGCSDDGL
                     ELFINEIKGEFKELVYINKKDNGIYNAMNQALDTDQLEEGLVTFLNAGDIYSSNLTLE
                     KVLSLYNKSAGVIAIPVSVAGKEIQQTLITGYIVNNICHQATFYNISCPIVKNTLKFD
                     ERYLLCADFAVLIKLSLSTDIHYLTQVPPVIYETSGISSIRIYKRLYEKAVIIYSSDL
                     LLINKLFSLLAILKGGVLHVFNRNSIK"
     gene            2092..2928
                     /gene="wfgQ"
     CDS             2092..2928
                     /gene="wfgQ"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgQ"
                     /protein_id="AIG62672.1"
                     /translation="MFLIATLSSKENEIDECKESVVKQTIDKKQIIFEGFDNITAHKK
                     VYSLFNEAEKKYKYLCKLDADMRFCDQDVLRDIAFFFDGHPRVDHIILPVRDHFTGKH
                     ILGAHFFRNGVRWIENNETIFVDPNPALCNKRVILYRWKNRIEHCFNPHDQQSFMFGV
                     HRISKLLAAGEKEDYSQFVSQLYVLNNLKKEYVKKKKRMHLLAMNGVMAVANGSANFY
                     DYSHKIKNNTSIKYDSIDYSLDDYGNYIYLQFKKLTPKGKAKYFYRKLILILKAVIYG
                     YS"
     gene            2915..3940
                     /gene="wzy"
     CDS             2915..3940
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AIG62671.1"
                     /translation="MVILKSRLIFLFFFVFLVLMVFYKIEANYFTPDEIFYLFSDSML
                     TDVTVASRYKFVAYIFRFIYEYGEYISLLIVNITIVWFVINKLSKSSNNNDVNYLIYG
                     FLFPSVVFYASLFLRDFYVFILAMLFVYVRRGKHKLFFQWCIILAIGFLKIELAAIFM
                     CAMFISRIKISPFVIVYMFIICVLTWFLALHNQTLMGIYLNTLERFEPTVFSEYNTLF
                     FGLQQMEPTPLNGAINIIFSYINLLSPFLISWPSNTGNIFNVIMTVDSILFLGCIIIG
                     VLGFNTNKYYSDDLYRLSIFVLLLSIIYGYFMVTPMTSLRMHIHFVPFAILFFMGSRF
                     KICVRRY"
     gene            3922..5067
                     /gene="wfgR"
     CDS             3922..5067
                     /gene="wfgR"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferases"
                     /protein_id="AIG62670.1"
                     /translation="MCKKILIIGASPPPYTGQSISLEKLKNTLENDSDFKVRHINLAP
                     KFGHTTGKFNIIRCSQTIYILLSYIWNLLLFKPNIIYMTKGSSKWGFVRDYAFQLLMK
                     MFSSNTKFVVHLKGGNYDSFYESSTELLKKCIVNFLKNCDSIIVLGPSLIKMYDFCPD
                     IKSKIRVVFNALPQDTVNNYVKENTDTVNVVFLSNLIISKGYLDVLQASLEVSKFNIH
                     FHFAGDFMLSPDDEDDKGDLKKRKEEFNNFIINNGLENNITYHGVLVGNKKNDLLKLG
                     DIFILPTYYHVPSYTISIIEAMSFRNAIISTNYRSIPDIVYEGRNAIFVKPKKPEDIV
                     NAITYLYENRNVLIAYQNNSYEYYHKKHIWSKHYSAMKEIFISEKAK"
     gene            5064..6182
                     /gene="gmd"
     CDS             5064..6182
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="AIG62669.1"
                     /translation="MKKAFITGITGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERVD
                     HIYQDRHNQNPNFFLHYGDLTDTSNLVRLIKEIQPDEVYNLGAQSHVAVSFESPEYTA
                     DVDAMGTLRLLEAIRICGLEKKTRFYQASTSELFGLVQEIPQRETTPFYPRSPYAVAK
                     MYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLYLG
                     NMDSLRDWGHAKDYVRMQWMMLQQDHPEDFVIATGKQISVREFVRMSAKEVGLELEFS
                     GQGVDEIATVVNKTSDCAIGVSIGDVIVRVDPRYFRPAEVETLLGDPAKAKKVLGWEP
                     EITVEEMCAEMVASDLAKAKQHALLKSHGYDVAVSLER"
     gene            6186..7151
                     /gene="fcl"
     CDS             6186..7151
                     /gene="fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="AIG62668.1"
                     /translation="MTKKSIYVAGHRGMVGSAICRQLSLRDDIELVVKTHKELDLTVQ
                     KDVDAFFEQEKIDQVYLAAAKVGGIYANNTFPAEFIYQNLMIESNIIHSAHKAGIQKL
                     LFLGSSCIYPKFAKQPMNESALLTGILEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGINDNFHPENSHVIPALMRRFHEAKESGAPEVIVWGTGTPMREFLYVDDMA
                     AASVHVMELDEAIYQQNTQPMLSHINVGTGVDCSIREMAETMASVVGYQGKIVFDVTK
                     PDGTPRKLMDVTRLKNLGWQYRYNLHEGLSLTYKWFIENINSFRG"
     gene            7154..7615
                     /gene="nudD"
     CDS             7154..7615
                     /gene="nudD"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="AIG62667.1"
                     /translation="MNKRLERELFKKIVEHTPLISIDLIIRNDKGEAQLGQRLNRPAQ
                     NYWFVPGGRIYKDESFEIAFKRITFEEVGVQISLNDALFLGVYEHFYNDNFSEAEFST
                     HYVVHGYEIQLKPQQLHLPTVQHNSYKWFDVVTLLNSTTVHQYTKNYFIPR"
     gene            7621..9027
                     /gene="manC"
     CDS             7621..9027
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AIG62666.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLNSRLTMLQETLRRL
                     DKVEHKPALVICNESHRFIVAEQLRKEGLKHSGILLEPVGRNTAPAVALAALQAMVTG
                     DDPILLVLAADHEIQNEDNFIAAVLAAKNFAEQGKLVTFGIVPTSPETGYGYIKSGEY
                     LDGKGYKVAAFVEKPELHVAQRYISDGGYLWNSGMFMFRASVFIDELKKFRPDILASC
                     QSSLSSSTQDLDFIRLDNSSFSCCPEESIDYAVMEKTAEAVVVPLNAQWSDVGSWSAL
                     WEISSKDQSGNAIRGDVLVEDATDSYLYSQHRLIGAVGVKDLVVVETKDAVLVAHKDK
                     VQQVKNIVAQLKKNNRTEYLQHREIFRPWGSHDTIAEGARFQVKHVIVLPGHITAKQI
                     HYHRTEHWIVVSGTAKVHLEDKAYLVSENESTYIPVGVPHAIENPGKIPLEIIEVRSG
                     VYLEEDDVIRVSSSGVGY"
     CDS             9027..9773
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="glycosyltransferase"
                     /protein_id="AIG62665.1"
                     /translation="MRISIITATYNSEKTLFDTLLSLEKQTHPDIEYIVVDGASKDNT
                     IKLIKSNSTKVSKIICEPDKGIYDALNKGIQAASGDVIGFLHSDDLLAYDDAIADIAK
                     TFESTGCDAIYGDLEYVAQNDTTKRIRLWKSGSFSRFKMKVGWMPPHPSFYMKRECYS
                     QFGSFSLDYRISADYDSLLRYILKQRISIAYLPKVLVKMRVGGISNRSVSSMAKKSME
                     DIRVMKHNGIIWPIALVYKNISKLPQFIKK"
     gene            9779..11203
                     /gene="manB"
     CDS             9779..11203
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="AIG62664.1"
                     /translation="MLNVKKIINDSNIAFGTSGARGLVIDFSHDVCAAFTHAFLSVID
                     DKYNFNKVALAIDNRPSSYEIAQACAYAIKQHGFEVEYHGVIPTPALAHYSMQKNIPC
                     IMVTGSHIPFDRNGLKFYRPDGEITKEDELAIINSEYTFSPVGVLPHLETSTQGADYY
                     LERYVSLFNPEILKGKRIGVYEHSSAGRDLYAPLFNQLGAEVISLGRSDEFVPIDTEA
                     VSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKT
                     LAIPVSCNTAIEESNKFASIQRTKIGSPYVIAAFADLAKQFDSVAGFEANGGFLLASD
                     LQINGKELKSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRLKNFPSDSSQ
                     QIIKNAISSPNNFFNSLGYESLSCSAIDETDGARFILNNGDIIHLRPSGNAPELRCYA
                     EASDENQAKQYVTNVLGNITSLIS"
BASE COUNT     3609 a   1752 c   2204 g   3877 t
ORIGIN
        1 acatgcatgc agtgctctgg tagctgtaaa gccaggggcg gtagcgtgga aaatattgag
       61 aatgtttgca tggtcattaa aagcagtaat ggatttgggc gtattagaat aaatatatta
      121 ctaggttttt tttcgaagtt tggtagtttt atattaagtt atatatcatt acctatagca
      181 ctgagctatt tgggtgttaa ttattatggt gtatggatag taattttctc agtagtagca
      241 tggatttata attttgatat tggtataggt ctaggtttaa aaaatagttt aaatgatgcg
      301 ctggtaaaaa aaaattataa gttagcaaat gaattgattg ctactgcgta tatatttctg
      361 atagcagtaa gtatattatt gctattgata tcatttctat tggttttttc tattaaccta
      421 gattctattt ttagtatgtc taatattaga gagagtgaaa taagaactac attacttgtt
      481 agtgtttttt ttacaattgc taattttatt ttgcttttat ataaacaatt gttttttgcc
      541 gtaaatttat cggggttcgc tgcggtaacc acaatattgt atcaagtttt ggtaatcgtt
      601 tttcttttag ttgcaaagtg ttttatagaa ccttcattgt taatagtggc aattatatat
      661 ggcgtgtcaa atttatttgt aggagtgttc tttacattgt atttctttaa gagaaatgat
      721 gtattaaagt ttagtttttc taattacaaa aggaatcata taaaatccat tggtggagta
      781 gggattaaat tttttatcat tcagatgtgt atgcttatga tttttacgtg tgataatatt
      841 atcatatcaa aatatcttgg tcctgaatat gttacaagct atagtcttat cttaaaaacg
      901 tatcaggctt ttattatgat aagttatata attcttactc cttattggac cttatttgcg
      961 gaagcttatt atcaaaaaaa cacggaatgg atattaaaac aatataagaa attatatact
     1021 ctgtttttac ttatgattat agcagtcatt gcttattcat tatatattca acctttgctt
     1081 aagatatggc tccatactga attgaaattc aataagtata tgatttatgg attttgtata
     1141 tttgtaataa ttagagtttt cattgatctg catgctgttt ttttgaatgg gattgggaaa
     1201 ataaatgtcc agttagtgtt atatcttttc gcggcgatta taaatatccc gttggcaatt
     1261 atattaataa aacatttcaa catgggtagt gaggcagtaa tattttccgc atcaatagcg
     1321 attgcaccga tggcaataat attgccactt caaagtttag tattattcag gaatattgcc
     1381 aagtgaagct aacagtaatt accgttgttt ataattctat tgaaatgttt aaaaaaacat
     1441 atttttgctt atctcaacaa acctgtcaga attttacatg gataataaaa gatggctgtt
     1501 cagatgatgg tttagagttg tttataaatg aaattaaagg tgagtttaag gaacttgttt
     1561 atattaacaa aaaagataat ggtatctata atgctatgaa tcaagcgttg gacacggacc
     1621 agcttgaaga aggattagtt acttttttaa atgctggaga tatttactca tctaatctaa
     1681 ccttagagaa ggtcttatct ctttataata aatccgctgg ggtaattgca attcccgttt
     1741 cagttgcagg caaagaaata caacagacgt taattacagg atatatagtt aacaatattt
     1801 gtcatcaagc aaccttttat aatatatcat gcccaattgt aaaaaataca ttgaagtttg
     1861 acgagcgata tttattatgc gcagatttcg ctgtgttgat taaattatct ttatccaccg
     1921 atatacatta tttaacacaa gtaccacctg taatttatga aacgtcaggc atttcttcta
     1981 ttagaatata taaaaggtta tacgaaaaag cagtaataat ttacagttca gatttattat
     2041 taataaataa gcttttttct ttgttggcca ttttaaaagg aggtgtattg catgttttta
     2101 atcgcaactc tatcaagtaa agaaaatgaa atagatgaat gtaaagaaag cgttgttaaa
     2161 caaactattg ataagaagca aatcattttt gagggttttg ataatataac cgctcataag
     2221 aaagtttatt cactttttaa tgaagctgaa aaaaaatata aatacttgtg taagttagat
     2281 gctgatatgc gattttgtga tcaggatgtg ctaagagaca ttgctttctt tttcgatggt
     2341 catccaagag ttgatcatat tatcttacct gttagagatc actttacggg taagcatata
     2401 ttaggtgccc atttttttag aaatggagta agatggattg aaaataatga aactatattt
     2461 gtagacccaa atccagcttt atgtaataaa cgcgttatac tttatagatg gaaaaataga
     2521 atcgaacatt gctttaatcc tcatgatcaa caatccttca tgtttggagt tcacagaata
     2581 tcaaaactct tagctgcagg agaaaaggaa gattacagtc aatttgttag ccagttatac
     2641 gtattaaata atttaaagaa agaatacgta aaaaaaaaga agcgaatgca tttattagca
     2701 atgaatggtg ttatggctgt agcgaatgga agtgcaaatt tttatgatta ttcacataaa
     2761 attaaaaata acacaagtat aaaatatgat tctattgatt attcactaga tgattatggt
     2821 aattatattt acttgcagtt taaaaaacta acgccaaaag gtaaggcgaa atacttttac
     2881 cgcaaactca tattaatatt aaaggcggtg atatatggtt attcttaaat caagattaat
     2941 atttctattc ttttttgttt ttttagtact aatggttttc tataaaatag aggcaaatta
     3001 ctttacgcca gatgagatct tctatttatt tagcgactca atgttaacag atgttacagt
     3061 agctagtcgc tataagtttg tagcgtatat atttagattt atttatgaat atggtgaata
     3121 tatctctcta ctgattgtaa atataacaat agtatggttt gtaataaata aactttcaaa
     3181 gtcatcaaat aacaatgatg ttaattattt aatttatggt tttttgtttc catccgttgt
     3241 tttttatgcg tctcttttcc tccgtgactt ttatgttttt attttggcaa tgctttttgt
     3301 gtatgttagg cggggtaaac ataagttatt ttttcaatgg tgtatcattt tagcaatcgg
     3361 atttcttaaa atagaattag cagcaatatt tatgtgtgca atgtttatat caaggattaa
     3421 aattagtcct tttgtaattg tttacatgtt tatcatatgc gttctcacat ggttcttggc
     3481 tttgcacaac caaacattga tgggaatata tcttaataca ttagaacgat tcgaaccaac
     3541 tgttttttct gaatacaata ctttattttt tggattgcaa caaatggaac cgactccgtt
     3601 gaatggtgca ataaatatta tttttagtta tattaatttg ctttctcctt ttctaatatc
     3661 ctggccatcg aatacaggaa atatatttaa tgttataatg acagtagatt caatcctttt
     3721 cttgggttgc attattattg gtgttttggg atttaatacc aataaatatt attcagatga
     3781 tctttacagg ttatctatat ttgttttgct actgtcaatt atttacggtt attttatggt
     3841 aacgccaatg acttcattgc ggatgcatat ccatttcgta ccttttgcaa tactattttt
     3901 tatgggatct aggtttaaaa tatgtgtaag aagatattaa ttattggcgc ttcccctccc
     3961 ccttatacgg gtcaaagtat ttctttagaa aagttgaaaa atactttaga gaatgattct
     4021 gattttaaag tcagacatat caaccttgcg ccaaaatttg gtcatacgac tgggaaattt
     4081 aatataatac gctgttcaca gaccatttat atattgcttt cgtatatatg gaatctgctt
     4141 ttatttaaac caaatattat atatatgacc aaaggaagtt caaaatgggg atttgtaagg
     4201 gattatgcat tccaactatt aatgaaaatg ttttcttcaa atacgaaatt tgttgttcat
     4261 ttaaaaggtg gaaattatga ttcgttttat gaaagttcaa cagaactttt aaaaaaatgt
     4321 atagttaatt ttttaaaaaa ttgtgatagt ataatagtat taggtccctc attaataaaa
     4381 atgtatgatt tctgtcctga tattaagagc aaaatacgtg ttgtatttaa tgcattgcca
     4441 caagacactg taaacaatta tgttaaagaa aatacagata cagttaatgt tgtattttta
     4501 tctaatttga taatttctaa gggatattta gatgtattgc aagcaagttt ggaggtaagt
     4561 aagtttaata tccactttca tttcgctggt gattttatgc taagccctga tgatgaagat
     4621 gataaagggg atttaaaaaa aagaaaagaa gaatttaata attttataat taataatgga
     4681 ctagaaaata atataacata tcatggtgtg ttggtaggta ataaaaaaaa tgacttactt
     4741 aaattaggtg atatatttat tctacctact tattatcatg tcccctcata cactatatcc
     4801 atcattgaag caatgtcatt tagaaatgca ataatttcaa caaattacag atctatacct
     4861 gatatagtgt atgaggggag gaatgcaata tttgttaagc ccaaaaaacc ggaagatatt
     4921 gtcaatgcta ttacgtactt gtatgaaaat aggaatgtat tgatagcgta tcaaaataac
     4981 tcttatgagt attatcacaa aaaacatata tggtcaaagc attatagtgc aatgaaagaa
     5041 atctttataa gcgagaaagc gaaatgaaaa aagcatttat tacaggcatt actggtcagg
     5101 atggctctta cttggctgaa ttccttcttg ataaagggta tgaagtgcat ggcattaaac
     5161 gtcgagcatc ttcatttaat acagagcgtg ttgatcacat ttatcaagat cgccacaatc
     5221 aaaatccaaa tttctttctt cattacggtg atttgacaga tacttcaaac ttagttcgcc
     5281 tcattaaaga aatccaaccg gacgaagttt ataaccttgg cgctcaatct catgtagcgg
     5341 tttcattcga atcacctgaa tatactgctg acgttgatgc gatggggact ttacgtctgt
     5401 tggaagcgat tcgtatttgt gggctagaga aaaaaacacg tttttatcag gcatctactt
     5461 ctgaactctt cggcttagtt caggaaatcc cacagcgaga aacaacacca ttctaccctc
     5521 gttcgcctta tgctgttgcg aagatgtatg catactggat tactgtaaac tatcgtgaat
     5581 cctatggaat gtacgcctgt aacggaattc tcttcaatca tgaatccccg cgtcgtggtg
     5641 aaacattcgt aacacgtaaa atcacgcgtg ctattgcaaa tatttcgcag ggaatcgaaa
     5701 aatgtctcta tcttggcaat atggattcac tgcgcgactg ggggcatgcg aaagattacg
     5761 tgcgtatgca gtggatgatg ttgcaacaag atcacccaga ggattttgta attgcaacag
     5821 gcaaacaaat ttccgtacgt gaatttgttc gtatgtcagc taaagaagtc ggtctggagt
     5881 tagaattctc cggacaaggt gttgatgaaa tagcaactgt tgtcaataaa acatctgact
     5941 gtgctattgg tgttagtatt ggggatgtga tcgttcgtgt tgatcctcgt tatttccgtc
     6001 ctgcagaagt tgaaactctt cttggtgatc cagcaaaagc gaaaaaagta ttgggttggg
     6061 aaccagagat tacagttgaa gagatgtgtg ccgaaatggt tgccagtgat ctggcgaaag
     6121 caaaacagca tgcactactg aaaagccatg gttacgatgt tgcagtttct ctggagcggt
     6181 aaggtatgac aaaaaaaagt atctacgttg ctggtcaccg aggtatggtt ggctctgcta
     6241 tttgccgtca attatcactg cgtgatgata tcgaattagt tgtcaaaaca cataaagaac
     6301 tcgatctaac cgtacagaag gatgttgatg cattttttga gcaagagaaa attgatcagg
     6361 tttatcttgc tgcggctaaa gttggtggca tttatgccaa caatacattt ccggcagaat
     6421 tcatctatca gaatctcatg attgagagta atattattca ttcagctcac aaggccggaa
     6481 ttcaaaaatt acttttttta ggctcaagct gtatttatcc taaattcgca aagcagccga
     6541 tgaacgagtc tgcgctttta acgggcatac ttgagccaac taatgagcca tacgcaattg
     6601 ctaaaattgc gggcataaaa ttatgtgaat cttataaccg tcaatatggc cgtgattatc
     6661 gcagtgtaat gcctactaat ctttatggca taaatgataa tttccaccct gaaaactctc
     6721 atgttattcc ggcactcatg cgtaggttcc atgaggcaaa agagagtggc gcaccagaag
     6781 ttattgtctg ggggaccgga acaccgatgc gtgagttttt atatgttgat gatatggctg
     6841 ccgcatctgt tcatgtaatg gaacttgacg aagcaattta tcaacaaaat acacagccta
     6901 tgttatctca tattaatgtt ggtacgggtg tagattgttc catacgtgaa atggctgaaa
     6961 caatggcctc tgtggtgggt tatcaaggta aaattgtttt tgatgttact aaacctgatg
     7021 gcactccgcg taaacttatg gacgttaccc ggctcaaaaa cttgggctgg caatatcgct
     7081 ataatttaca tgaaggctta tcattaacat acaaatggtt tattgagaat attaattctt
     7141 ttcggggata gttatgaata agagattgga acgtgagcta tttaaaaaaa tagttgagca
     7201 tactcctcta atctcgattg atctcataat tagaaacgat aaaggagagg cgcagctggg
     7261 gcagcgcctt aatcgcccag cacaaaatta ttggtttgtg ccaggagggc gaatttataa
     7321 ggatgagtca ttcgagattg catttaagcg gataacattt gaagaggtgg gcgttcaaat
     7381 tagtcttaat gatgccttat ttctaggggt gtatgaacat ttttacaatg ataatttttc
     7441 tgaagcagaa ttttctacac actatgtagt gcatggatat gaaatccaac ttaaacctca
     7501 gcaacttcac ctaccaacgg tccagcataa ttcctacaag tggtttgatg tagtaacgtt
     7561 gcttaatagc actacagttc atcaatatac caaaaattat tttataccaa ggtaatagat
     7621 atgctacttc ccgttgtcat ggccggtggt tctggtacca gattatggcc tctttcacgt
     7681 acactttatc cgaaacaatt tctgtctttg aatagtcgtt taaccatgtt gcaagagaca
     7741 ttgcggcggc ttgacaaggt cgaacataaa cctgctttgg tcatttgtaa cgaatcacat
     7801 cgctttatcg ttgctgaaca attgcgtaaa gagggtttaa agcatagcgg tattttgctt
     7861 gagcctgttg gtcgtaatac tgcgcctgct gtagcacttg cagcacttca ggctatggta
     7921 actggagatg atcctattct gttggttctt gctgctgatc atgaaatcca gaatgaggat
     7981 aatttcattg ctgcagttct tgcagccaag aattttgcag agcagggtaa acttgttacg
     8041 tttggtattg ttccaacatc cccagagact ggctatggtt acattaagtc aggtgaatat
     8101 ctggatggaa aaggttataa agttgcggct tttgttgaaa aaccagagct tcacgtagct
     8161 cagcggtata tatcagatgg cggttatctt tggaatagtg ggatgtttat gttcagagca
     8221 tctgtattta tcgatgaact gaaaaaattc cgaccagata ttttagccag ttgccaaagc
     8281 tccctgtcct cttcgacaca agatttagat tttatccgtc tggataactc ttcattttct
     8341 tgctgtcctg aagagtctat tgactatgca gttatggaaa aaacagccga agctgtcgtt
     8401 gttccattaa atgcgcaatg gagtgatgtt ggatcatggt ctgcattgtg ggaaataagt
     8461 tcaaaagacc aaagcggcaa tgccattcgt ggtgatgtat tggttgaaga tgctacagat
     8521 agttatcttt attcgcagca tagacttatt ggtgccgtgg gcgtaaagga tttggttgtt
     8581 gttgaaacga aagatgcagt attagttgct cataaagata aagttcagca agttaaaaat
     8641 attgtcgctc aacttaaaaa gaataatcga acagaatatt tacagcatcg ggaaattttt
     8701 cggccctggg gcagtcatga cactatagcc gaaggggcgc gctttcaggt gaaacatgtg
     8761 attgtattac ctggtcacat tactgctaag cagattcatt accatcgtac tgaacattgg
     8821 attgtcgtct caggtacagc taaagttcat cttgaagata aggcttacct tgtttctgaa
     8881 aatgaatcaa catatatacc tgttggtgtt ccacatgcta ttgaaaatcc tggcaagatc
     8941 ccgctcgaaa taattgaggt tagatcagga gtctacctgg aagaggatga tgttataaga
     9001 gtttcttctt ctggagtagg atactaatgc gaatttctat tatcacagct acttataata
     9061 gtgaaaaaac tctctttgat acattacttt ctctagaaaa gcaaacgcat ccagatattg
     9121 aatatatagt tgtagatgga gcatcaaaag ataatactat taagctgatt aagagtaata
     9181 gtacaaaagt ttcaaaaatc atttgcgaac ccgataaggg catttatgat gctctgaata
     9241 aaggtattca agcagcttcg ggtgatgtta ttggtttttt acattctgat gatttactag
     9301 cttatgatga tgctattgca gatatagcaa aaacatttga aagtacaggg tgtgatgcta
     9361 tttatggcga tttggagtat gttgcccaaa atgatacgac taaacgtatt agattatgga
     9421 aaagtggttc atttagtcgt ttcaagatga aagtgggttg gatgccgcca cacccatcat
     9481 tttatatgaa acgcgaatgt tatagtcagt ttggtagttt ttctttagat tatcgaatat
     9541 ctgctgatta tgattcatta ttacgatata ttttaaagca acgtatttca atagcatatt
     9601 taccaaaagt cttagtgaag atgcgtgttg gtggaattag caatcgttca gtatcttcaa
     9661 tggccaagaa gtcaatggaa gacattcgtg ttatgaaaca taatggtatt atctggccaa
     9721 tagctttagt gtataaaaat atatccaaac ttcctcaatt tattaaaaag taatcatcat
     9781 gttaaatgtt aaaaaaatca ttaacgatag taatattgcg ttcggtacta gtggtgctcg
     9841 cggtttagtg atcgattttt cccatgatgt ttgtgctgcg ttcactcatg cgtttctttc
     9901 tgttattgat gacaaataca attttaataa agttgcctta gccattgata accgcccaag
     9961 tagttacgaa attgctcagg catgcgcgta tgctataaaa caacatggat tcgaggttga
    10021 atatcatggt gtaattccga ctcctgcatt agctcattat tcgatgcaga aaaacattcc
    10081 ctgcataatg gtcacaggga gtcatatccc ttttgatcgt aatggcttaa aattctacag
    10141 acctgatggt gaaatcacta aagaggatga gctcgcaatt ataaatagtg agtatacatt
    10201 ttcgcctgta ggtgtattac ctcatcttga aacaagcact caaggtgcag actactacct
    10261 cgagcgttat gtttctcttt ttaatcccga gattttaaag gggaaaagaa taggggtata
    10321 tgaacattct agtgcgggac gcgatttata tgctcctctt tttaatcaat tgggtgcaga
    10381 ggtcatttcc ctcggtagaa gtgatgagtt cgttccgatt gatacggaag cagtaagcga
    10441 tgaagatcgt atacttgcaa gagagtggtc taaaaaatat aatcttgatg ctattttctc
    10501 aacagatggc gatggtgatc gtcctttagt tgccgatgaa aatggtgaat ggttaagagg
    10561 cgatattctg ggattactta ctgctattga acttaatatc aagacgttgg ctattccagt
    10621 gagttgtaat acagcaattg aagaatctaa taaatttgca agtatacaac gaacgaaaat
    10681 aggttctcct tatgtaattg cagcgtttgc agatcttgct aagcaatttg attcagtagc
    10741 tggttttgaa gctaatggtg gttttctcct tgcctccgat ttacaaatta atggcaagga
    10801 attaaaatcg ttacctacac gagatgctgt gttaccagca ttaatgctct taatagcttc
    10861 tcgcaatagt accatttctc aactgattaa taatcttcct cagcgattca cttggtcaga
    10921 tagacttaaa aacttccctt cagattcaag tcaacaaatt ataaaaaatg ccatatcgtc
    10981 acccaataat ttctttaata gtttaggtta tgaatcatta tcctgttccg ctattgatga
    11041 aacggatggt gcaagattta ttttaaataa tggtgatatt atacatctcc gtccttccgg
    11101 taatgctcca gaactccgtt gttatgctga ggccagtgat gaaaatcagg ctaagcaata
    11161 tgttacgaat gtgctgggaa atattacctc tttgatttct tgatattata ggtttatcta
    11221 cgcttatatg tgtgcatagg tttgattaca cgtagatacc ggtatacaga attgaagaac
    11281 ggtatttatt gcattaatga aattcagcac tacacacatt cgtgcaactt gagataacat
    11341 ctcaatcata ttcaagtcgc gcatacatcg cggtgaacat cccctgacag gagtaaacaa
    11401 tgtcaaagca acagatcggc gtcgtcggta tggcagtgat gg
//
