LOCUS       KP710591               15751 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain 22-56 serotype OX13:H10 O antigen gene
            cluster, complete sequence.
ACCESSION   KP710591
VERSION     KP710591.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15751)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15751)
  AUTHORS   Baranzoni,G.M., Yan,X., Fratamico,P.M., Debroy,C., Tebbs,R.S.,
            Liu,Y., O'Connell,C.D., Swimley,M. and Matheny,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2015) Molecular Characterization of Foodborne
            Pathogens, United States Department of Agriculture, 600 E Mermaid
            LANE, WYNDMOOR, PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="22-56"
                     /serotype="OX13:H10"
                     /db_xref="taxon:562"
     misc_feature    414..15236
                     /note="O antigen gene cluster"
     CDS             complement(416..1453)
                     /codon_start=1
                     /transl_table=11
                     /gene="orf1"
                     /product="acyltransferase family protein"
                     /protein_id="AJR19374.1"
                     /translation="MNSREHRNNCFDIIRLFAAIIVIISHQYSLSGLSEPTFLGMTTL
                     GGIGVIIFFAISGFLITKSCINSERFDLFLLKRVRRIFPALIPCSIFMYLVLGVYLEW
                     DTLDEYISFSILKNILNVITLQGAPAHSGIFNGFIHPYSLNDSLWTLPLEFTCYLILG
                     CIVFLSKNTLRVILITFIALLFISIYFLFNKTDIIFYGIVLQAFPVRALSFFTGAIMA
                     LTLNYWYNKRCISYLLIISTIVLMAVAYKNEINVIGYINIAIITIAIGHIFKDNLIAG
                     RFDYSYGVYIYSFPIQQIIINKLTLGFFEGTFISIVVSLIFGFFSWHIVESKFIIKNK
                     KIEQYSKVLSS"
     gene            complement(1477..2289)
                     /gene="wbbD"
     CDS             complement(1477..2289)
                     /gene="wbbD"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AJR19375.1"
                     /translation="MNHSVAIIMSVYFSEKPDYLKQSLSSLFTQTMNADIYLYVDGDL
                     NELLEDVIKGFKVHSNFFVIRGKVNKGLAFALNTLIDITLNKGYKYIARMDTDDISRS
                     NRIEQQFRFMEENKGIDVLGTFCHEFGSEYALELKAVPIKHDELKKYSIIRCPFIHPT
                     VIFRSSIFKDGIRYPLNTKFTEDMALWFCLLEEGYHFHNLPEVLLDYRIDENTFSRRR
                     GLRKAISEFSIRFKYMIKLREFNINNSIVLLGKFCFHILPLFIIRYAYKKYR"
     gene            complement(2363..3730)
                     /gene="algC"
     CDS             complement(2363..3730)
                     /gene="algC"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="AJR19376.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLVQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDIKLMIMKTDEI
                     LRILN"
     gene            complement(3734..5182)
                     /gene="manC"
     CDS             complement(3734..5182)
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AJR19377.1"
                     /translation="MSDVPLIAVVMAGGTGSRLWPLSREHYPKQFLQLSGENTLLQST
                     LLRLSPLSCETPLVITNEQHRFVVAEQLRQINQLSDNIILEPCGRNTAPAIALSAFTA
                     LKRNEQQDPILLVLAADHVINKTDVFCNAIKNSISIVEQGNILTFGIIPHYAETGYGY
                     IKKGNVIKESDSGVGNKFYQVEQFVEKPNRSRAEEYISSDKYLWNSGMFMFKASVYLE
                     ELKKYRSDIYDICEKTVSSSYHDLDFIRLSKDVFQNCPSESIDFAVMEKTKRCIVYPL
                     DIGWSDVGSWQSLWDVSDKTPTGDVCKGDILTYNTKNNYIHSESALVAAVGVEDIVIV
                     QTKDAILVSKKSEVQDVKQIVQMLKKQERSEYLSHREEFRPWGKFDAIEQGERYKVKK
                     IVVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLNNKTVLVTANESVYIPLGATYSLENP
                     GVIPLNLIEVSSGDYLGEDDIVRQKERYKIDD"
     gene            complement(5175..5675)
                     /gene="gmm"
     CDS             complement(5175..5675)
                     /gene="gmm"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="AJR19378.1"
                     /translation="MFLRQEDFATVVRSTPLLSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEDELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFNHAPSAVIGLDK
                     KDVKNV"
     gene            complement(5678..6643)
                     /gene="fcl"
     CDS             complement(5678..6643)
                     /gene="fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="AJR19379.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHVAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAEGELLQGSLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(6646..7764)
                     /gene="gmd"
     CDS             complement(6646..7764)
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="AJR19380.1"
                     /translation="MKVALITGITGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVD
                     HIYQDPHTCNPKFHLHYGDLTDTSNLTRILCEVQPDEVYNLGAMSHVAVSFESPEYTA
                     DVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAK
                     LYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLG
                     NMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAEQVGIKLRFE
                     GTGVDEQGIVISVTGEDAPAVKPGDVIVKVDPRYFRPAEVETLLGDPTKAHEKLGWKP
                     EITLREMVSEMVANDLVAAKKHSLLKSHGYDVAIALES"
     CDS             complement(7786..8586)
                     /codon_start=1
                     /transl_table=11
                     /gene="orf8"
                     /product="glycosyltransferase"
                     /protein_id="AJR19381.1"
                     /translation="MSKVCCRLAGGLGNQIFQYGASLLISKKSNVKYIELDDSELNNY
                     KASRSNELPHYFKMTNSNVENKFLLKLRLPRINILANLTDRFVGDLNFNRVLYQSLTN
                     KSYYLDGYFQNCLDQTLFDEMLELLKKDYLYSHLSIKPNVCAIHIRGGDFLTKKYSGI
                     ASTNYYLEQLNKIKEMNSVDQFVIVTDDKNYATEIAKKISIKYFFSDGNMLDDFLLLA
                     QSQFKVLSNSTFAIWASALGYKPGCIVLAPQKLNIHDTRNFILPGELI"
     gene            complement(8597..9397)
                     /gene="wbuO"
     CDS             complement(8597..9397)
                     /gene="wbuO"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="AJR19382.1"
                     /translation="MNKYILYLPFGYTYLSRIKTIPKLISFILTIPIPCFVFFYFGLL
                     SSSERDLSIGAGFVVSYVCVYIFYENGYIQNDIKTVKKERNPTLRIVGELYDFIDTNY
                     YSIILLRTLFFVFLLFLVLYVTPYIIIIKIISYAFFTSVIYYFHNNIRNAFKTVTFFI
                     LSSSRFIFPILIFSSVMVPEKLAYIPLSVLIYTLPALLIYSTKSFGLMHFIIGNENKY
                     KIIYYFIMAVVFLLLYLLSLNNSVWLIMLIIFFYMMILVFIKEIIVNK"
     CDS             complement(9397..10056)
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="AJR19383.1"
                     /translation="MKLAIFDICGTLYYDNTTYSFLIWLSNKGIIKNKKSIMNAPYIL
                     RALNKIYSSLSGIDLYRNIQIKQLAGFSYYDLTRWAIEFIDSLEDNRILSTFALMEEY
                     KRKGYNIVIASATIDPIAKAIANKLSVEYYSSKLSYSNNMCSGKLEKDLLLQKKVMLS
                     KLLDKVEVAIVVTDNFTDIELVKMCDHCHIIVHNDKNIRKWSKLLNNNISKIDFIKKN
                     V"
     CDS             complement(10061..11203)
                     /codon_start=1
                     /transl_table=11
                     /gene="orf11"
                     /product="glycosyltransferase"
                     /protein_id="AJR19384.1"
                     /translation="MKLFYRVNDYHPFDIKDSSAASSVRHRVDRVCRCLPDATKSIIY
                     SIVKEKNVLLIKRNVNNTENNQQCITYFVPSNPLGILIKIFLSLCLMIYLLFKTLKKE
                     NYLLIYNLLPVHIFPYLIASYIYPSFLKKTIFQVEELYSMYNFSFIKNLLNLHSEKFV
                     FRNGLNFIVVNENVKKIIKQKKAKSIVDYGYNTRNSKAYNKLSVSDTIVYTGRLDYVG
                     GVEVLLEAISNLPSDIRNKFVITGYGGLKEQVVAHANELNIKFKGFLSEADYDSLLSS
                     AKISINPLRSKCNFSLYSFPSKVLQYLEYGCVVISSGIEQPELEYYFSDELFSYKNDD
                     ARTLSMLIMEINSRNFDKVKIIDKYHVFMKNKEKKLRAFFLSVFEG"
     gene            complement(11178..12341)
                     /gene="wzy"
     CDS             complement(11178..12341)
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AJR19385.1"
                     /translation="MQTVNNHFYKTLLLLSFFSAGFSLYYVFIPLLIISLILSMIKNR
                     TLYAGCTAASYNVLFIVLFVFSVFSIGSELLILESPVKYAMYMIFLIVCLPLLLINNF
                     EYAWYMLFFFILGVFFRAESIVVYSFLDPSGVYGYGKLLDPFNHEEINSPGISNSLAI
                     VFMFFFVAYKNTLSLKYLLIFIYPLILLSSIFLGGRAFFIIALVCITYKYSRNFKIKT
                     LIGLTILICLGCIFSLLVKEIPFFDKYFDLIFERFGSEGLKSARFELIQDGITKFLIH
                     PLGGFTPNASEYSGYWYHNIYLDSARVAGVISLIPFILSNLIVLISYLGYRNYRKRIK
                     NPYFFISLITLIIMCQDVVLEGNMMLLISYALFTTMAIQSRRYKKSETLLQSK"
     gene            complement(12341..13021)
                     /gene="cat"
     CDS             complement(12341..13021)
                     /gene="cat"
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="AJR19386.1"
                     /translation="MGLLSRIKSHLKKRKLNKSHLNIYVDVFSYIDESSTLDGCNRIT
                     GKSSIYDSHIGKYSYVAGASIGNATVGKFCSIAKGAKIGGLGAHPTTFISSHPIFYST
                     KKQCGVTFTSKDYFLENADTILGNDVWVGTNAIIMDGVTIGDGAIIGAGAIVTKDVPP
                     YAIVVGVPAIIKRYRFPTEQIEILKKIKWWDWPEQDLKKNSFLFREDFNESICELAKL
                     YNKVKRLD"
     CDS             complement(13005..13934)
                     /codon_start=1
                     /transl_table=11
                     /gene="orf14"
                     /product="glycosyltransferase"
                     /protein_id="AJR19387.1"
                     /translation="MNNISPVVVFVYSRPEHTKKMLSALSKNSLSECTDLIIYSDGAK
                     SEKDKPGVYAVRQLIRKLTGFRSITIVEQEQNVGLAQNIIQGVTKVVKQYGRVIVLED
                     DIITSPYFLKYMNDALDMYADIKEVASISGSSYPVTINNNLESTYLLRIPLCWGWATW
                     DDRWRMFKKDINSVNLISKDVKNYINFDGAFNFFDQATLNAKGKLNTWFIFWYITLAS
                     HKWLTLFPSKVMAKNIGYDGTGENCNGDDIYKDILIDNEPLELELKKDLTESYDYYLA
                     HLHYFRKNQQNFIYKIYSFVKKIVKKRCYGTSK"
     gene            complement(13947..15236)
                     /gene="wzx"
     CDS             complement(13947..15236)
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AJR19388.1"
                     /translation="MLKTLIKKTKLNFEIQKIILNAGWLLFDKFIRLMFGLLVGVWIA
                     RYLGPGTYGQLAYIVTLVSLFQVVVSLGMDTILVREITCNKNEAAQILGSAIFMRFIV
                     GCISIVLLIGGVTIFNGAYFGVLTLLASGGLLLQCTDTIDIWFQSQSQSKRTVIAKLC
                     SYTITNILRIILIIYNAPLWTFALLLSIDASLSALALTFAYKTFKTAHRWHVSLLRCK
                     ALLSESWPFLISGISIILYMRIDQVLIKNIMGEKPLGIYSAAIQFSTLWNFIPLTLSV
                     SLAPYVARKKKESEQIYYQTLCNIFLIFSIIGWCITLTIITLSPFIIEFMLGANYKDG
                     VGVLSIHVITNLFICLGISQSLWIVNERKGTFILYKSITGLVVSVIANIILIPLYGIK
                     GAAIASVLSQITASTIFNLCFAKKIFFMQLKSLFFLR"
BASE COUNT     5072 a   3133 c   2633 g   4913 t
ORIGIN
        1 cataactgcc ataccgacga cgccgatctg ttgctttgac attgtttact cctgtcaggg
       61 gtagtcacca taattcttat gcagctataa gctgattctg atgttaactc atattagata
      121 ataaacaata taagtacacc aggttaagtc aattaaatta aagctcaata aacactagag
      181 ttaatatatt atttttcgtg gcaaaaagca tatacgttca tccaaaatac attggatgtg
      241 tgtatagcga atcaaaaaac aacaattaat ttaagcatat acttttctac tacagaacac
      301 catgttttaa gttaatttat tttaatcgtt aaaatcgcgt tttttcttaa tgtactttca
      361 tttcatatct ctacttaaat attaatcaaa aaatagagtt gtgatattta agatattatg
      421 aagagagtac tttactatat tgttctattt tcttattctt gataatgaat tttgattcta
      481 ctatatgcca tgaaaaaaag ccaaaaataa gtgatactac gatactaata aaagtacctt
      541 caaaaaaacc aagggttagc ttatttataa taatttgttg tattggaaat gaatatatat
      601 atactccgta ggaataatcg aagcgtcctg caattaagtt gtctttaaaa atatgtccaa
      661 ttgcgatcgt gattattgcg atattgatat aacctatcac attgatttca tttttataag
      721 caacagccat gagcactatt gtggaaatta taagcaaata acttatgcat cttttgttat
      781 accaataatt cagagtaagc gccataatgg cacctgtaaa aaaagataat gcacgaacag
      841 gaaaagcttg taatacaata ccataaaata ttatatcggt tttattgaaa agaaaataaa
      901 tagatataaa aagcaacgcg ataaaggtaa ttaatattac tcttaaagta tttttagata
      961 aaaatacaat acatcctaat atgagataac aagtaaactc cagagggagt gtccataaac
     1021 tatcatttag agagtatgga tgtataaatc cattaaaaat tcctgaatgt gctggagctc
     1081 cttgtagggt tataacattg agtatgtttt ttagtataga aaaactgata tattcatcta
     1141 aagtatccca ttccaaatac actcctaata ctagatacat aaaaatggaa cagggaatta
     1201 atgcaggaaa tattcttcta acacgtttta ataaaaacaa atcaaatctt tcactattga
     1261 tacaggactt tgtgattaaa aaacctgata tggcaaaaaa tatgataacg ccaatacctc
     1321 ctaaagtagt cataccaaga aaggttggtt ctgacaatcc agaaagtgaa tactgatgag
     1381 aaataattac aataattgct gcaaacaatc taattatatc aaaacagtta tttctatgtt
     1441 ctctactatt cattttatga actttcaaag attaatttaa cgatattttt tatatgcgta
     1501 tcgaataata aataaaggca atatatgaaa acaaaacttt ccaagcaaaa caatactgtt
     1561 attaatatta aactccctga gttttatcat atatttaaaa cgaatagaaa actcacttat
     1621 tgcttttcta agcccccttc tccgagaaaa tgtattttca tcaattctat aatctaataa
     1681 tacctcaggt aaattatgga aatgatatcc ctcctcaaga agacaaaacc ataacgccat
     1741 atcttcagta aatttagtgt tcaacggata acggattcca tctttaaaaa ttgaacttct
     1801 aaatataaca gttgggtgta tgaaggggca acgtattata ctatacttct tcaactcatc
     1861 atgcttgata ggtactgcct ttaactctaa tgcatattcg ctgccgaatt cgtgacaaaa
     1921 agtaccaagc acatcaattc ctttattttc ctccatgaat ctgaattgct gctcaattcg
     1981 attcgaacgt gaaatatcat cagtatccat ccgagcaata tacttataac ctttattcaa
     2041 agttatatca atcaaagtat tgagtgcaaa tgcaaggcct ttgttaacct taccacgaat
     2101 aacaaagaaa ttagagtgaa ctttaaaacc tttaattaca tcttctaata attcattcaa
     2161 atcaccgtct acatagagat aaatatctgc attcatagtt tgagtaaaca aactagaaag
     2221 gctttgtttc aaataatctg gtttctcaga aaagtagacg ctcattatta ttgctacact
     2281 atgattcatc ctatataaat atccatttag tttatttatt ataattaaga ccatgtttta
     2341 taacatggcc ttatgctcaa agttagttta atattcttaa tatttcatct gttttcataa
     2401 tcatgagttt aatatcgcca cgcgattcta cattcaaccg caccaccggt tcggtgttgg
     2461 aggaacgcag gttaaagcgc cagtcggcaa aggtcatgct gatgccatcg gtgcgatcca
     2521 ccgccagcgc ctcgcggcta aaatgctgtt cgacgcgatt aatcgcctca acgggttgca
     2581 ccagtttgct gttgatctca ccgcttgccg ggaatgcagc catgcggtcg cgcaccagtt
     2641 cgcccagcgt ttttcctttc aggcacacca gttcggcgac cagcagccac gggatcatgc
     2701 cgctgtcgca gtaagcgaaa tcacggaagt aatggtgggc gctcatttcg ccaccgtaga
     2761 tggcgtcttc cttgcgcata cgttctttaa taaaggcgtg tccggttttc gacatcaccg
     2821 gggtgccgcc tgcggcagtc actacatcaa cggtgttcca ggaaagacgc ggatcgtgga
     2881 tgatcttcgc gccgggattt ttttcgagga acgcttctgc cagcaggccg acaatgtagt
     2941 agccctcgat aaactgtcct ttttcgtcaa acaggaaaca gcggtcaaaa tcgccatcaa
     3001 aagcaatacc catatccgcg ccgtgtttaa tgaccgcatt gcgggtatcg tcgcggcatt
     3061 ccggcagcaa tggattagga ataccgttgg ggaaattgcc gtccggcgtg ttgtgtactt
     3121 tgatcaattc cacgggtgcg ccgagggctt taaagcgggc ttcgatggcg tccaccaccg
     3181 gacccgccgc accgttccct gagttgatta ccagcttgag cggcgtgagg tttttgacat
     3241 tgatataacc gaacaggtga tcaacatatg cgtcacgtag gttgatttgc tgatagcgac
     3301 cacgtttggt ttcatcgacg ggagggaagt cgttggcttc tgccagacgc tggacgtcgc
     3361 gcagtccggt atcaccgctg atcgggcgag ccccttcgcg caccagcttc atgccgttat
     3421 aatccatcgg gttatggctg gcggtaactt cgatgccgcc atccacaccg agatggaacg
     3481 tggcgaaata gatctcttca gtgccggaca tgccgatatc cagcacgtca acgcccgcat
     3541 cctgtaaccc ttttgccagc gccagtttta aggtttcgct ggtgagacgg acatcaccgc
     3601 ctaaaacaat ggttttcggt ttgagaaatt cgccataggc acgcccaata cgccaggcga
     3661 tatcttcatt cagttcttcg cctaattttc cgcgaatatc gtaggcttta aagcaggtta
     3721 attttttcat ctattagtca tctattttgt aacgttcttt ttgacgaaca atatcatcct
     3781 cacctaaata atctccggaa ctaacctcaa taagattaag aggaattacg ccaggatttt
     3841 caaggctata agtagcgccc agaggaatat aaacggactc attagcagta actaagacgg
     3901 ttttattatt taaagttact tttgctgtgc ctgaaagaac aatccaatgc tctgaacgat
     3961 gatgatgcat acgtaaggat agcccttccc ctggtttaac gacaatcttt ttgactttat
     4021 atcgttcccc ctgctctata gcatcaaatt tcccccaagg ccgaaactcc tcgcgatgtg
     4081 atagatattc agaacgttct tgctttttga gcatttggac aatttgttta acatcctgaa
     4141 cttcagattt ttttgaaaca agaattgcat ctttcgtctg aacaataact atatcctcta
     4201 cacctacagc ggctactaag gctgactccg agtgaatata attatttttt gtattatagg
     4261 tcaggatatc acctttgcaa acgtcccctg taggagtttt gtcactaacg tcccacaacg
     4321 attgccaaga tccaacatcg ctccaaccaa tatcaagggg atagactata caacgctttg
     4381 ttttttccat tactgcaaaa tcaattgatt ccgatggaca attttgaaaa acatctttcg
     4441 agagtcgtat aaagtcaaga tcatggtagg aagaagaaac tgttttttca catatatcgt
     4501 atatatcaga tctgtatttt tttaattcct ctaaatatac tgatgcttta aacataaaca
     4561 tcccactatt ccaaagatat ttatctgagg aaatatactc ttcagccctt gagcgattag
     4621 gtttttcaac gaattgttct acttggtaaa atttattacc aactccactg tctgattctt
     4681 taattacatt cccttttttt atataaccat aaccggtctc tgcataatga ggaataatac
     4741 caaaagttaa gatatttcct tgttcaacaa ttgaaatgga atttttgata gcgttacaaa
     4801 aaacatctgt tttgtttatg acatggtcag ctgcaagaac taaaagtata ggatcttgtt
     4861 gttcattacg cttcaaagcc gtaaatgctg aaagtgctat agctggtgca gtatttcttc
     4921 cacatggttc aagaataatg ttatcgctta attggtttat ttgacgcaat tgttctgcga
     4981 ccacaaaacg atgctgctca ttagttatta ctaatggagt ttcgcaagaa aggggggata
     5041 gtcgtaataa agttgactgt aataaagtat tctcacctga taattgcaga aattgctttg
     5101 gatagtgctc tcttgagagg ggccaaagac ggctacccgt accaccagcc attacaacag
     5161 caatcaacgg tacatcagac atttttgaca tcctttttat ctaaaccaat aacagcactt
     5221 ggagcatgat taaaataggc tcgactgtta gcgtgaacat tgtcactggc gagcagcgcg
     5281 tccggcgtca gccagcggta atcgtcgtgc tgctcatccg gcagtaacag ctcatcttcc
     5341 gcgactctga agcgaaaacc gagcaccaca tagtgagtgg tgaaatccgt gccggagaag
     5401 ttatcgtcat aaaagtgctg ccagacaccg taaaactggc ctgctgttat cggcagacgc
     5461 agccccagtt ccgccatcgt caaccgctca aatgcggctt ccagcgtttc gtctttctgc
     5521 acgcgccctc ccggcacaaa ccagtaaccc tgtgccgggc ggttggttct tttgccaagc
     5581 agaaactcgc cgcggctgtt ctcaacaata aagtcgagag agagcagcgg cgtggagcgc
     5641 actaccgtgg caaagtcttc ctgacgtaaa aacatcatta cccccgaaag cggtcttgat
     5701 tctcaaggaa ccactggtaa gtgctggcaa gccccgcttc cagtgagatt tcgtgatacc
     5761 agccaagctg atgcaggcgc gtcacatcca gcaatttgcg cggcgtgcca tccggtttgc
     5821 tggcatcgaa aaccacccga cctttgtaac ccaccacttt agcgatggtt tgcgccagct
     5881 cgcggatagt gcagtcaacg ccggtaccga cgttaatgtg cgacagcatc ggctgggtgt
     5941 tttccagcca gacttcgtgc gccagctcca tgacatgaat gctcgccgcc gccatatcat
     6001 cgacgtgcag aaattcgcgc atcggtgtac cgctgcccca caccaccacg tccggcgcat
     6061 tctgtgccgt cgcctcgtgg aagcgacgca gcaatgcagg gatcacgtgc gagttactcg
     6121 ggtggaagtt gtcgtgcggc ccgtacaggt tggtcggcat gacagagcgg taatcgcgtc
     6181 catactggcg gttgtaagat tcgcacagtt tgatcccggc gattttggca atggcgtaag
     6241 gctcgttggt cggctccagc gagccctgta acagctcgcc ttctgccatc ggctgttttg
     6301 ccagttttgg gtagatgcag gacgatccga gaaacagcag tttgttcacg tcgttctgat
     6361 gcgcgacgtg aatgatgttg ctctcaatca tcatgttctg gtagatgaaa tctgccggat
     6421 atgtgttgtt ggcaacaatg ccgcccactt tcgccgccgc cagatagacc tggtcaatgc
     6481 gctcgctggc aaagaaatca tgcaccgcgc ggctgtccag caggttcagt tcgtcgcggg
     6541 tgcgtaatac cagttccaca tcaccgcgct gttcgagctg tcgcttgatg gcagaaccga
     6601 ccatcccgcg atggccagcg ataaaaatgc gttgtttact catgcttatg actccagcgc
     6661 gatcgccacg tcgtagccgt gagatttcag cagagagtgt tttttcgccg ctacgaggtc
     6721 attagccacc atttcagaca ccatctctct gagggtgatt tccggtttcc agcccagttt
     6781 ttcgtgcgct ttggtcgggt cgccgagcag cgtttctact tcagcaggac ggaagtaacg
     6841 cgggtcaacc ttaactatta catctcccgg tttaacggct ggggcatcct caccagtaac
     6901 agaaatcact atcccctgct catctacacc ggtgccttca aagcgcagtt tgatccctac
     6961 ttgctccgct gccatttcca cgaactgacg tacggagtac tgaacaccgg tagcgataac
     7021 gaaatcttct ggctgttcct gctgcagcat catccactgc atttttacgt agtctttggc
     7081 atggccccag tcgcgcaggg aatccatatt gccgaggtac aggcacgact ccagtccctg
     7141 ggcgatattg gcgattgcgc gggtgatttt gcgggtaaca aaggtttcgc cacggcgcgg
     7201 ggattcatgg ttaaagagaa tgccgttaca ggcgtacatg ccgtaggatt cgcggtagtt
     7261 aacggtgatc cagtaggcat acagtttggc gaccgcatac ggagatcgcg ggtagaacgg
     7321 cgtggtttct ttctgcggaa tttcctgcac caggccatac agttcagaag tcgaagcctg
     7381 atagaaacgg gttttctttt ccagaccaag gaagcggatc gcctccagta aacgcagtgt
     7441 gcccatcgcg tcgacgtctg cggtatattc cggtgactca aaagaaaccg caacgtggct
     7501 cattgcgccc aggttgtaca cttcatccgg ctgcacttca cacaaaatgc gcgtcaggtt
     7561 ggaggtatct gtcagatcgc cataatgcag atgaaatttc gggttgcagg tgtgtggatc
     7621 ctgataaata tgatccacac gctcggtgtt gaatgacgat gcacgacgct taataccatg
     7681 tacttcgtaa cctttttcca acagaaactc tgccaggtaa gaaccgtcct gtccggttat
     7741 gccagtaatt aaagcaactt tcatttttga ataactcctt aaaatttaaa ttaattcacc
     7801 aggtaagata aaatttctcg tatcatgaat atttagcttt tgaggcgcta atacaataca
     7861 tcctggtttg tatcctagcg cagaagccca tatagcaaat gtactatttg acaacacttt
     7921 aaattgggat tgtgccagta ataaaaaatc atcgagcata tttccatcag aaaaaaagta
     7981 tttaatagat atttttttcg ctatctcagt cgcataattc ttatcatctg tcacaattac
     8041 gaattgatcc acagaattca tttctttaat tttattcaat tgctcaagat aataattcgt
     8101 cgaagcaata ccactatatt ttttagttaa aaaatcacct ccgcgaatgt gaatcgcaca
     8161 tacgtttggt ttaatactga gatgcgaata tagataatct ttttttaaca actcaagcat
     8221 ttcatcaaaa agagtttgat ccaaacaatt ttggaaataa ccatctaaat aatagctttt
     8281 gtttgtaaga ctttgataga gcactcgatt aaaatttaag tcaccaacaa atctatctgt
     8341 aaggtttgct aaaatattta tacgcggcaa acgcaattta agcaaaaatt tattttctac
     8401 atttgaattg gtcattttga aataatgtgg caattcatta cttcttgatg ctttataatt
     8461 gtttaactcc gaatcatcaa gctctatata tttcacatta ctttttttgc taattagtaa
     8521 tgaggcgcca tattgaaaaa tttgattacc taatcctcca gctaacctgc agcatacctt
     8581 cgacatcttg atacccttat ttattaacaa taatttcttt tatgaacaca agtatcatca
     8641 tataaaaaaa tattattagc atgataagcc aaacactatt atttaatgaa agaagataaa
     8701 gcaacaaaaa aacaaccgcc ataataaaat agtaaattat cttatattta ttttcattac
     8761 caataatgaa atgcattaaa ccaaatgact tagtcgaata tattaataat gcaggtagag
     8821 tatatattaa aactgaaagt ggtatatatg ctaatttctc tggtaccatc acggatgaaa
     8881 aaattagaat tggaaatata aaccgtgatg aagacaagat aaaaaaagtt actgttttaa
     8941 aggcatttcg tatattatta tggaaataat aaattacact agtaaaaaaa gcatatgaga
     9001 ttattttaat tattatgatg tagggagtaa catataaaac aagaaaaagc aaaaaaacaa
     9061 aaaaaagagt tctaagcaaa ataatactat aataattagt gtcaataaaa tcatataact
     9121 caccaacaat tctcaatgtt ggatttctct ctttcttaac ggtttttata tcattttgta
     9181 tgtatccatt ttcataaaaa atataaacac atacataact aacaacaaac cccgccccta
     9241 tcgaaagatc tctttctgaa gatgaaagca gcccaaaata aaaaaatacg aagcacggta
     9301 taggtattgt aagtataaaa ctaataagtt ttggaattgt tttaatcctg gacaaatagg
     9361 tatatccgaa gggtaaatat aaaatatatt tattcatcaa acattcttct ttatgaaatc
     9421 aattttggat atattgttgt taaggagttt cgaccatttt cgaatatttt tatcattatg
     9481 aactataata tgacaatgat cgcacatttt aactaattca atatcggtaa aattatcagt
     9541 aactactatt gcaacttcta ccttatcaag taatttagat agcatcactt ttttttggag
     9601 caaaaggtct ttttcaagtt taccactaca catattattg gaataactca attttgatga
     9661 gtaatattcc acagataatt tattagcaat ggcttttgct ataggatcta tagtggcgct
     9721 agcaattaca atattatatc ccttacgctt atactcttcc attaaagcaa aagtacttaa
     9781 tatacgatta tcttcaagac tatcgataaa ttctattgcc catctagtta aatcataata
     9841 cgaaaatcca gccaattgtt ttatttgaat attcctgtat aaatcaatac cgcttaatga
     9901 tgaatatatt ttattgagag cacgtaaaat ataaggcgca ttcattatgc tttttttatt
     9961 tttaattatt cctttgttag ataaccagat taaaaaagaa tatgttgtat tgtcataata
    10021 taatgtgccg catatatcaa aaatagccaa tttcacagaa ttatccctca aatacagata
    10081 agaagaaggc gcgtagcttt ttttctttat ttttcataaa gacgtgatat ttatctataa
    10141 tttttacttt atcgaagtta cgcgaattta tttccataat taacatactg agtgtccgag
    10201 catcatcatt cttatatgaa aataattcat cagaaaaata atattccaat tctggctgct
    10261 ctattccact tgagataacg acacatccat attccagata ttgaagtact ttcgaaggaa
    10321 atgaatataa agaaaaatta cattttgatc ttagtggatt aatactaatt tttgcactag
    10381 ataataatga atcataatca gcttcgctaa gaaatccttt aaatttaata ttcagctcat
    10441 ttgcatgagc aaccacctgt tcttttaatc ccccataacc tgttataaca aatttatttc
    10501 taatatcact aggtaggtta cttattgctt ctaaaagtac ctcaaccccc ccaacatagt
    10561 ctaaccttcc agtataaaca atagtatctg atacacttaa cttattgtaa gccttgctat
    10621 tccttgtatt gtacccataa tcaactattg attttgcttt tttttgctta ataatttttt
    10681 taacattctc attaacaact ataaagttaa gaccatttct aaaaacaaac ttttctgagt
    10741 gaagatttaa aaggttctta ataaacgaaa aattgtacat gctatataat tcttcaacct
    10801 gaaaaattgt ttttttcaaa aaactcggat atatataaga cgctataaga tacggaaaaa
    10861 tatgaactgg caataaattg tatataagga gataattttc tttctttaaa gttttaaata
    10921 ataaatatat cattagacag agcgaaagaa atatttttat aagtatccct aaaggatttg
    10981 aaggaacaaa atatgttatg cactgttggt tgttttcagt attattcaca tttcgtttaa
    11041 tcaataaaac atttttttct tttacaattg aatatattat tgatttggtg gcatccggaa
    11101 gacatctgca aactcggtcg actctgtgtc gaactgaact agctgcagaa ctatccttta
    11161 tgtcaaaagg atgataatca tttactctgt agaagagttt cactcttttt atacctcctg
    11221 gattgaatag ccattgttgt aaataaggca tagctgatta gtaacatcat attaccttca
    11281 agtacaacat cctgacacat gataattaaa gttataagtg atataaaaaa atatggattt
    11341 tttattcttt ttctataatt cctatatcct aaatagctaa ttaacacaat taaatttgaa
    11401 agtataaatg ggataagcga tataacccca gcgactctgg cgctatcaag ataaatatta
    11461 tgataccaat acccactata ttcgcttgca tttggagtaa acccccccag tggatgaatt
    11521 agaaattttg taataccatc ctgtataagt tcaaatctag ccgatttcaa tccttcactt
    11581 ccaaatcgct caaatattaa atcaaaatat ttatcaaaaa aaggaatttc tttaactaat
    11641 aaagaaaaaa tgcaaccaag gcatattaat atagttaatc ctatgagcgt ttttatttta
    11701 aagttacgac tatatttata agttatacat actaatgcaa taataaagaa tgctcgcccc
    11761 cccaaaaata ttgatgataa taaaattaat ggatatataa atataagtaa gtacttcaag
    11821 ctcaatgtat ttttatatgc aacgaagaaa aacataaaaa caattgcgag tgagttacta
    11881 atgcctggag aatttatttc ctcgtgatta aatggatcta gcaatttacc atatccataa
    11941 acgccggaag gatctaggaa gctataaaca actatggatt cagctctaaa aaagacacct
    12001 aatataaaaa agaaaagcat gtaccaagca tattcaaaat tattaattaa gagtaatggt
    12061 aaacatacta tcaaaaatat catatacatt gcatacttga caggggattc aagtattaat
    12121 agttctgaac ctatagaaaa cacactaaat acaaataaaa caataaataa cacattataa
    12181 cttgcagcag tacatcctgc gtataatgtt ctatttttaa tcattgatag aattaatgaa
    12241 attattaata acggtataaa tacgtaatac agactaaacc ctgcagagaa aaaacttaat
    12301 agaagtaaag ttttataaaa atgattattc actgtttgca ttaatctagc cttttaactt
    12361 tattatataa tttagctaac tcacagatac tttcattaaa atcctctcga aataggaatg
    12421 aatttttttt taggtcttgt tcaggccaat cccaccattt aatttttttt aaaatttcaa
    12481 tctgttcagt tggaaaacgg tatcgtttta taattgccgg aacaccaaca acgattgcat
    12541 atggtggaac gtcttttgta acaattgcac ctgcacctat tatggcacca tcaccaattg
    12601 ttaccccatc cataatgatg gcattcgtac ccacccatac atcatttcct aatattgtat
    12661 ccgcattttc taaaaaataa tccttactag taaacgtaac cccacattgt ttcttagtag
    12721 aataaaagat aggatgagaa gatatgaaag ttgttggatg cgcaccaagc cctcctattt
    12781 tagctccttt tgcaatactg caaaattttc ccactgtcgc atttcctatg cttgctccgg
    12841 caacataact atatttccca atatgagagt cataaattga tgattttcca gttattctgt
    12901 tacaaccatc taaagttgaa gactcatcaa tataagaaaa aacatcaaca tatatattta
    12961 agtgactttt attcaatttt ctttttttta aatgtgattt aattctactt agaagtccca
    13021 tagcacctct tttttactat ctttttcaca aatgaataaa ttttataaat aaaattttgt
    13081 tgatttttcc tgaaataatg taagtgagct aaataataat cataactttc agttaagtct
    13141 ttttttagtt ctaactcgag aggttcatta tcaattaata tatctttata aatatcatct
    13201 ccattacagt tttcacctgt cccatcataa ccgatatttt ttgccattac ctttgatggg
    13261 aatagagtta accacttatg agaagccagt gttatatacc aaaatataaa ccaagtatta
    13321 agtttacctt tagcatttag agtggcttga tcaaaaaaat taaatgcacc atcaaagttt
    13381 atataatttt ttacatcctt acttattaaa tttacggaat taatatcttt tttgaacatt
    13441 ctccatctat catcccaagt agcccacccc caacacaagg ggatccttaa aagatacgtt
    13501 gactctaaat tgttatttat ggttactggg taactactac cagagatcga ggctacttct
    13561 tttatatctg catacatatc taaagcatca ttcatatact tcagaaaata gggtgatgta
    13621 attatatcgt cttctaagac aataactctt ccatattgtt tgactacttt tgtaacgccc
    13681 tgaattatat tttgagcaag acctacattt tgttcctgct caacgatagt gatacttctg
    13741 aaaccagtta acttcctgat aagttgccgt acggcataaa caccaggttt atctttttct
    13801 gatttagcac catcagaata gataatcaaa tctgtacatt cagatagact attcttactt
    13861 aatgctgaca gcattttttt tgtatgctca gggcgtgaat agacaaaaac aactacaggg
    13921 gaaatattat tcactttttg ttccatttat ctaaggaaaa acaaactttt caattgcata
    13981 aaaaaaatct tcttcgcaaa acacagatta aaaattgtag aagctgtaat ttgtgagaga
    14041 actgacgcta tcgctgcgcc tttgatgcca tatagcggaa taagaatgat atttgctata
    14101 acactgacaa caagccctgt aattgacttg taaaggatga aagtaccctt tctctcatta
    14161 acaatccaga gactctgtga aattcctaaa catatgaata aattggttat aacatggata
    14221 gaaagaacgc caacgccatc tttataattg gcacctaaca taaattctat tatgaatggc
    14281 gataaagtaa taatagttaa tgttatacac catccgataa ttgaaaatat taaaaatata
    14341 ttacacagag tttgataata tatctgttca ctttcttttt ttttccttgc cacataagga
    14401 gctaaactta ccgataaagt caatgggata aaattccata atgtagaaaa ttgtattgca
    14461 gctgaataaa tacctagcgg tttttctccc attatatttt ttatcaaaac ttgatcgatc
    14521 ctcatgtata atatgataga tattccgctt attaaaaaag gccatgactc tgacagtagc
    14581 gccttgcatc gtaataaact tacgtgccat ctatgcgctg ttttaaatgt tttataagca
    14641 aacgtcaaag ctagagcaga taatgatgca tcaatactta gcaaaagagc aaaagtccat
    14701 aatggtgcat tatatattat gaggataatc cgtaatatat tcgtaattgt gtaagagcat
    14761 agctttgcta taacagttct tttactttgg ctctgacttt gaaaccaaat atctatagta
    14821 tctgtgcatt gtaataataa tccgccactt gccaataatg ttaatactcc aaaatatgca
    14881 ccattgaata tagttacacc accaataagt aatactatgc ttatacaacc aacaatgaaa
    14941 cgcataaata tagcgcttcc taaaatttgg gcagcttcat ttttattgca cgtaatctca
    15001 cgcactaaaa ttgtatccat tccgagagat acaacaactt gaaataaact tacaagagta
    15061 acaatataag ctagctgacc atatgtccca ggccctagat agcgtgcaat ccagacacca
    15121 actaacaagc cgaacattaa tcgaataaac ttatcaaata acaaccatcc agcatttaaa
    15181 attatttttt gtatttcaaa gttgagtttt gtttttttta taagagtctt cagcattaag
    15241 cacctgatag atataagtaa aatatggcct atattgcttg tataataaat ttgtagtatt
    15301 agctttaacc agacacgcta ccgcccctgg ctttacagct accagtgcac tgcgtgcatt
    15361 ctcataagat gttacagact ctgctactaa cgtcgcaaag ctctcaaaaa tttaaacgcc
    15421 aattgtctaa ccaaacgagg tcagacacaa ggaaaatccg aaaatttttg gtctcactaa
    15481 agagatataa ctcattgtta ttattacatt tacataagaa acggcaggaa cattcttatt
    15541 atgtaagcat ctctgctttt gaatgttcac tgccgttcta ttcttattca atcccccata
    15601 tatgggatgg ttaattttta ttcacttaac agcttctcaa tccctttgcg gaacttcgcc
    15661 ccttctttca aattgcgcag tccatacttc acaaacgcct gcatataacc cattttttta
    15721 ccgcagtcgt aactgtcacc ggtcatcagc a
//
