LOCUS       KJ710507                6380 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain OX18 serotype OX18:H12 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ710507
VERSION     KJ710507.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Swimley,M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC v. v. 5.1
            Assembly Name         :: de novo
            Coverage              :: 50
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="OX18"
                     /serotype="OX18:H12"
                     /db_xref="taxon:562"
     misc_feature    1..6380
                     /note="O-antigen gene cluster"
     gene            69..1340
                     /gene="wzx"
     CDS             69..1340
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AIG62369.1"
                     /translation="MKLINKILKNHKSSLFSIFSLGFKFFTGPLAIILIASRFTVIEQ
                     SIYYTFMSISSIQWVLEMGVSTCLIQFMAAEKEKRATYSKIAFIFYSAISILLYIIFY
                     FYSKWIFSDTDIEIWRQPWILFSVGVCINVFTTFIYIIEEGGGAYEKVYFAKFISSIL
                     YSLSLLLSLFLGLKLYSLGIAQFFLFATPIILYRSKIYSYYHSFRINNETNIRIIIKD
                     IVKFQVKLSLVWITGYLYWNFFTIYFYKYVSVDLAGKYGASNTIFGAISLAMLSILQT
                     KRALIGNLISRNAILEVKKIVSQCVSLSVAGFALGSIAIISVMWLWPNPLFSRFLSLD
                     SLIALLVFRFFIHLQELILIYLRMYKDEPIYILTVFNYLTTPCLVVFLVDRFSITYFF
                     YIMAFVQFLFFLCYVIKAKLYIREKYYNDLL"
     gene            1327..2517
                     /gene="wzy"
     CDS             1327..2517
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AIG62370.1"
                     /translation="MIFYNLLLVIAIFFSSLVNHGNAKFIPVLLIILLINPRNYTYKN
                     MLCLIAGGGLITASAIYFNLYGFLDVHPEKVEAFWGNYFSYLALIMFIVMYIQIASNK
                     NIYSKLYNAIKYVIIVHVLIITLQGIVFYTTGTYIDFVEPFTGEASRYENYLESASFA
                     KIRFTGLYVEPSTYAAAIVTLVLTKSILATQLNKGNDYFDYIALLSAILTFSSASIIY
                     ANIAILYLIYTTVLNSKLKSLALIAVSMILCGTFFIFHNEIFQIAIDTVTKFQERGGM
                     RLGLIHYIFSEREGFIYWFGPNIFGLEKELYILTHNLNNGPRLMGAENDSGLFVYFVV
                     MFGVFSIIPLLATFLFILNKKKQLSIPFVIVGLTKISFMHYVFWIFIITLIYSLNAKK
                     INDD"
     gene            2507..3259
                     /gene="waaX"
     CDS             2507..3259
                     /gene="waaX"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AIG62371.1"
                     /translation="MMIKVYIISLKKDKKRRIDLINQLEQMNIPFVIIDAVEGALLAK
                     AKIDKFNTMTKFRYRRRVGKNEIGCSLSHQKVYRDIIKNNIEWAIILEDDVSIKVDFF
                     SLIKNKIKNFNKDNLYILGGQNGLLSKRMQQTSIFKRLELDKRFFFAKTINSEKYIFR
                     TCCYMINNSVARKIVEKFKDDFFIADDWLYLKRKKVFKHIFMSDMIDHPIDLSNSSIE
                     LERENRNVNKFKIISILKKLNCFLRNIYKFGI"
     CDS             3261..4157
                     /codon_start=1
                     /transl_table=11
                     /gene="orf4"
                     /product="glycosyltransferase"
                     /protein_id="AIG62372.1"
                     /translation="MKTNITIIVPTYKRPASLKKLINSLNTQIDTAFKLVIVNDDPDN
                     AISLTELRHLKFDVTIYNNSTNIGPASSRNKGATLADTEWICFLDDDDEFRNDKISVL
                     RKVIMSNFDADVIINAALINLTDENINYKTMPNPENYKNNISMFNSLGGAPLLTIKRD
                     FFLNIGMYDPELKALEDYDLNIRIIKAHSKIIFINDELTICNYTSTVDTVSKIVANNI
                     DAMQRIIKKYPDIFSVETKKVFSWIYSTLAYKSLLTKSRLTAIKLYILSLHYSFNVKT
                     CVSLLISLFNPRLLFLMRSKKK"
     gene            4168..5430
                     /gene="tolB"
     CDS             4168..5430
                     /gene="tolB"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB"
                     /protein_id="AIG62373.1"
                     /translation="MPLQYNFFERMVGKFLQSTPHLRNFIKNIYQRINYLIYKKKYKY
                     KMYSDYKLRIPPTKDASFFGYYDRTCWHKDGNLVLLNKIRNGKMIICIWDLTKDSIIE
                     VSETLTWNWQQGALATWLNTDFPAFIFNSLENNKLVSKVFMFDKGKWIYKETHPYPVQ
                     SVSSNLLMSSINYITLQKLRPDYGYDVEANNFKYPTDIEADGLFIYDLVENKLLFLIT
                     LKEIIELNFTNSMNSAKHKINHVIFSPNGENLIFMHRWITKNGKFSRLILLNLITKTM
                     KIIFDNRMVSHYQWRNDKQFVAWARSEQFGDKYHLVDIENNEIKILPHDSNINNFGDG
                     HPSFNNEGSMIITDTYPGRDRMRSLLLFDMSTSKLTKIGDFLSPWAFNGVGRCDLHPR
                     WSPDGKKVAIDSAHSGQRQLILIEIDKK"
     gene            5434..6252
                     /gene="amsE"
     CDS             5434..6252
                     /gene="amsE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AIG62374.1"
                     /translation="MSDTENFSVLMSVYNKESSINLRQALNSIWMDQHLRPDQIVLVK
                     DGMLGDELNDVIIYFSKIIPCLDVVSLPSNMGLAYALNEGLKCCKYQLVARMDSDDIS
                     LPTRFAEQIAYMSSNKNIAVLGSQVDEYDMLMENKVGTRVVPIKHEDIVRFAKRRNPI
                     SHPSAVFRKEIILSVGGYPLFERAQDFALWSIMLTRGHMFHNSPRVLVNMRTGDGMMN
                     RRGYEYLKNELRLLNFQKEIGLLSYFDFICNAMTKCIVRLAPKRIKKLLYSKFR"
BASE COUNT     2215 a    807 c   1080 g   2278 t
ORIGIN
        1 agaatctacg cagtgcactg gtagctgtaa agccaggggc ggtagcgtga tatatgtagg
       61 caagagaatt gaaattaatt aataaaattt taaaaaacca taaatcttct ttattttcga
      121 tattttcact gggctttaaa ttttttactg gacctttagc tataatatta attgcttcaa
      181 ggtttactgt tattgagcaa agcatttatt atacttttat gtcaatatca tcaattcagt
      241 gggttcttga aatgggggtt tctacatgtc tcattcaatt tatggcagca gaaaaagaaa
      301 aaagagcaac atattctaaa attgctttta tattttactc agcaatatct atattattat
      361 atataatttt ctatttttac tctaaatgga ttttttctga tacagacata gaaatttggc
      421 gtcaaccttg gatattattt tcagttggag tatgtataaa tgtatttaca acatttatat
      481 atataatcga agaagggggg ggggcgtatg aaaaggtgta ttttgcaaag ttcattagct
      541 ctatattata ttctctttct cttttgctat ctttattttt aggtctgaag ttatattcat
      601 taggtatcgc gcagtttttt ctatttgcaa caccaataat cttatatcga tcaaaaatat
      661 atagttacta tcattctttc cgaataaaca atgagactaa tattagaatc atcataaaag
      721 atattgttaa attccaagtt aaattatcac tggtgtggat tacagggtat ttatactgga
      781 atttttttac aatatatttt tataaatatg tctctgtaga tttggcaggg aagtatgggg
      841 catctaatac tatatttggt gctatatcat tagcaatgct atcaatatta caaacaaaaa
      901 gagcattgat aggtaatttg ataagtagaa atgctatctt ggaagtgaaa aaaattgtat
      961 ctcaatgtgt gtcattaagt gtggccggat ttgcactggg aagtatagct attatatctg
     1021 tcatgtggtt atggccgaat cccttatttt caaggttttt atcattagat tctctaatag
     1081 cattacttgt atttcgtttc tttatccatc tgcaagaatt aatattgatt tatctgagga
     1141 tgtataaaga tgaacctata tatatattaa cagttttcaa ttatcttaca acaccatgcc
     1201 ttgttgtatt tttggtggat aggttttcca tcacatactt tttctatata atggcatttg
     1261 tacagttctt gttttttctt tgttatgtaa taaaagcaaa gctttatatt agagagaaat
     1321 attataatga tcttttataa tttattattg gtaatagcga tttttttttc ctctttggtt
     1381 aaccatggga atgcgaaatt cataccagtt ttattaataa ttttattaat taaccctaga
     1441 aattatacat ataaaaatat gttatgctta atagcaggtg gaggattaat aactgcatca
     1501 gcaatatatt ttaacttata tggtttttta gatgtacatc ctgagaaagt cgaggccttt
     1561 tggggaaatt atttttctta tcttgcattg ataatgttta tcgtgatgta tatacagata
     1621 gcctcaaata aaaatatata ctcaaaatta tataatgcaa ttaagtatgt aattatagta
     1681 catgtcttaa ttattacctt gcaaggtatt gttttttata caaccggtac atatatagac
     1741 ttcgttgaac cattcactgg agaagcaagt cgttatgaga attacttaga atcggcttct
     1801 tttgccaaga ttcgattcac agggttgtat gtagaacctt caacatatgc tgccgcaatt
     1861 gttacgctag tattaactaa atcgattctt gccacgcaat taaataaagg gaatgactat
     1921 tttgattata ttgcattact atctgcaatt ttaacgtttt caagtgcttc tataatatat
     1981 gctaacatag ctatattata tttaatatat acaactgtac tgaatagtaa attaaaatca
     2041 ctagcactaa ttgctgtttc aatgattttg tgcggaacgt ttttcatttt tcataatgaa
     2101 atatttcaga ttgcaattga tacagttaca aagtttcagg aaaggggggg gatgcggtta
     2161 ggtttaatcc attatatctt tagtgaacga gaaggtttta tatattggtt tggacctaat
     2221 atttttggtt tggaaaaaga actttatatt ttgactcata acctgaataa tgggccaaga
     2281 ttgatgggag ctgagaatga ttcaggtttg tttgtatatt ttgtagtgat gtttggcgtt
     2341 ttttctatta tcccattgct tgcaaccttt ttatttattt taaataaaaa aaaacaacta
     2401 tctattccgt ttgtgattgt agggctaact aaaatatctt ttatgcacta tgttttttgg
     2461 atttttataa ttacattaat ttactcgtta aatgctaaga aaataaatga tgattaaagt
     2521 atatattatt tcattgaaaa aagataagaa aagaagaatt gatttgatca atcaattgga
     2581 acaaatgaat attccatttg ttattattga tgctgtggaa ggagctttat tagctaaagc
     2641 aaagatagat aagtttaaca caatgacaaa attccgatac agaagacgtg ttggcaaaaa
     2701 cgaaataggc tgttctttaa gtcatcaaaa agtttaccgg gatataataa agaataatat
     2761 agaatgggca attattttag aggatgatgt tagtattaaa gttgatttct tttcattaat
     2821 aaaaaataaa atcaaaaact tcaataagga taatttatac atattaggtg gtcagaacgg
     2881 gttgttatcg aagaggatgc aacaaacaag catttttaaa agacttgagt tagataaaag
     2941 attttttttt gctaaaacaa ttaatagtga aaagtatatt tttagaacct gttgttatat
     3001 gattaataat agtgttgcaa gaaaaatagt tgaaaaattt aaggatgatt tttttatagc
     3061 tgatgattgg ctgtatctta agaggaaaaa agtttttaaa catattttta tgagtgatat
     3121 gatagatcat cctatcgatt tatccaatag ttcaattgaa ttagagaggg aaaatagaaa
     3181 tgtaaataaa tttaaaataa tttctatttt aaaaaagctt aattgttttt tgcgaaatat
     3241 ttataaattt ggtatataaa atgaaaacaa acataacaat aatagttcca acttacaagc
     3301 gtcctgctag tcttaaaaaa ttaattaata gtttaaatac tcaaatagat acagctttca
     3361 aattagttat tgttaatgat gatccagata atgctattag cctaacagaa cttaggcatc
     3421 ttaaatttga tgttactata tataataatt ctacaaatat cgggcctgct agttcgcgca
     3481 ataaaggagc tacgttggct gataccgagt ggatatgctt tttagatgat gatgatgaat
     3541 tcagaaatga taaaatatca gttcttagaa aagtaattat gtcaaatttt gatgccgatg
     3601 tgatcattaa tgcggcctta ataaatctaa ctgatgaaaa tattaattat aaaacgatgc
     3661 ccaatccaga gaactataaa aataatatat ctatgtttaa ttcgttagga ggggctccat
     3721 tattaacaat aaagcgagat ttttttctta atattggaat gtatgatcca gagttgaaag
     3781 cattagaaga ttatgatctc aatattagaa taatcaaagc gcactcaaaa attattttca
     3841 taaatgatga attgactatt tgtaactata catcaacggt agatacggta tctaagatag
     3901 ttgcaaataa tattgatgca atgcagagaa ttataaaaaa atatccagat attttctctg
     3961 ttgaaactaa aaaagtgttt tcttggattt attcgacctt agcatataaa tcattgctaa
     4021 caaaatctcg ccttacggcc ataaaattgt atatattatc attacattat agttttaatg
     4081 ttaaaacttg tgtttctctt ttgatttctt tgtttaaccc tcgtcttttg ttcttgatgc
     4141 gaagcaaaaa aaaataacgg agtaaaaatg cctctacaat ataatttttt tgaaagaatg
     4201 gttggtaagt ttttgcaatc aactccacat ttgagaaatt ttataaaaaa tatataccaa
     4261 agaataaatt atttaattta taagaaaaag tacaaatata aaatgtattc agattataaa
     4321 ttgcgtattc ctccaactaa agatgcatca ttttttgggt attatgacag aacatgttgg
     4381 cataaagatg gtaacttggt tctattgaat aaaattcgaa atggaaaaat gatcatatgt
     4441 atttgggatc ttacaaaaga tagcatcata gaagttagtg agacattaac atggaactgg
     4501 caacaagggg ctttagctac gtggcttaat acagattttc cggcttttat atttaactct
     4561 ttagaaaata acaagctcgt ttcaaaagtg tttatgtttg acaaaggtaa atggatttat
     4621 aaggaaacac atccatatcc agtgcaatcc gtttcatcaa atcttcttat gtcttcgatt
     4681 aattatataa ccttacaaaa acttaggcct gattacggct atgacgttga ggcaaataac
     4741 ttcaaatatc ctaccgatat agaggctgat gggcttttta tttatgatct tgtagaaaac
     4801 aagctattat tcttaattac actaaaagaa attatagagc tcaattttac aaatagcatg
     4861 aatagtgcga agcacaaaat caaccatgtt attttttctc ccaatgggga gaatttaatt
     4921 tttatgcatc gctggataac taaaaatggc aagttttcta ggttaattct actcaattta
     4981 attactaaaa ccatgaaaat tatatttgat aatcgaatgg tgtctcatta tcagtggcgt
     5041 aatgacaaac aatttgttgc ttgggcgaga agtgagcagt ttggtgataa atatcacttg
     5101 gttgatattg aaaataatga aattaaaata ttacctcatg attctaatat taataatttc
     5161 ggagatggac atccatcatt caataatgaa ggctctatga ttattactga tacttatcct
     5221 ggtagagata gaatgagaag cctacttctc tttgatatgt ctacctcaaa attaacaaaa
     5281 ataggggatt ttctatcacc ttgggctttt aatggtgtcg gccgatgtga tcttcatcca
     5341 agatggtccc ctgatggtaa aaaagttgct atagatagcg cccattcagg acaaagacaa
     5401 cttatattaa ttgaaataga taagaaataa attatgagtg acactgaaaa tttttctgtt
     5461 ttaatgtcag tatataataa agaatcatct ataaacttaa gacaagcgtt gaatagtatt
     5521 tggatggatc aacatttacg tccagatcaa attgttttag taaaagatgg tatgttaggt
     5581 gatgaattaa atgacgttat tatatatttt tccaaaataa ttccctgttt ggatgtcgtt
     5641 tcgctgccaa gtaatatggg gttagcttat gctctgaatg aagggcttaa atgttgtaaa
     5701 tatcaattgg tagctaggat ggacagtgat gatatatcat taccaacgcg gtttgccgaa
     5761 cagatagctt atatgagttc taataaaaat atcgcggtat taggctcgca agttgatgaa
     5821 tatgatatgt taatggagaa caaagtcggg acaagggtgg ttcctataaa acatgaagat
     5881 attgttcgtt ttgcgaaacg aagaaatcct ataagtcatc catcagcggt ttttcgaaaa
     5941 gaaataattt taagtgtcgg tgggtatcct ctttttgaga gagcacaaga ctttgctcta
     6001 tggtccataa tgttaactag aggacatatg tttcataact ctcctcgtgt gctcgttaat
     6061 atgaggactg gagatggtat gatgaacaga agaggatatg aatatttaaa gaatgagttg
     6121 cgtttgttaa attttcagaa agaaatagga ctccttagtt acttcgactt tatctgtaat
     6181 gctatgacta aatgtattgt aaggctagcg cctaagagga ttaaaaaatt actttattca
     6241 aaatttagat aatgcaataa acagttttta tttgtaaata tatagtaata attatcttaa
     6301 cttggtaagt ttgtcagaat ctaagacagg agtaaacaat gtcaaagcaa cagatcggcg
     6361 tcgtcggtat ggcagtgatg
//
