LOCUS       KP868751               12882 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain PF1912 serotype OX19:H36 O-antigen gene
            cluster, complete sequence.
ACCESSION   KP868751
VERSION     KP868751.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12882)
  AUTHORS   Yan,X., Baranzoni,G., Fratamico,P.M., Debroy,C., Tebbs,R.S.,
            Liu,Y., O'Connell,C.D., Swimley,M. and Matheny,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2015) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PF1912"
                     /serotype="OX19:H36"
                     /db_xref="taxon:562"
     CDS             complement(206..1576)
                     /codon_start=1
                     /transl_table=11
                     /gene="manB"
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="AKI84594.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVEFIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPVEAINR
                     VEQHFTREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESHGDVPLMEEKTKLI
                     LELLNK"
     misc_feature    complement(206..12770)
                     /note="O-antigen gene cluster"
     CDS             complement(1601..2347)
                     /codon_start=1
                     /transl_table=11
                     /gene="orf2"
                     /product="glycosyltransferase"
                     /protein_id="AKI84595.1"
                     /translation="MKVSIITVTYNSEKTLRDTLESIELQTYKDIEYIIIDGGSTDNT
                     LKLINEVSTRVTKCLSESDNGIYDALNKGINLSTGDIIGFVHSDDLLARPDIIETIVS
                     RFHETKADVVYGDLVFFEKNQIDIIKRYWRSGPFKRSKLSLGWAPPHPSFYMRRELYK
                     DDGYFDLSYRIAADYDQMARILKRDDIKVIYVPQVFVKMRLGGESTRIDNAISSTKEI
                     VEVMKNHNVNWKIAIIIRKISKLMQLFAHK"
     gene            complement(2431..3816)
                     /gene="manC"
     CDS             complement(2431..3816)
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="AKI84596.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLTSRLTMLQETLRRL
                     EGVEHRPALVICNEVHRFIVAEQMRNVNLANSGILLEPIGRNTAPAVALAALKAISSG
                     EDPILLVLAADHEIQDQKRFISSILAAKEFAEEGKLVTFGIVPTKPETGYGYIKTGEN
                     LNEYGFKVSAFVEKPELDVAKKYLEDGDYLWNSGIFMFRASVFIDELNKFRPDILKIC
                     QQALKSSTQDLDFIRIDNDSFSCCPEESIDYAVMEKTTEAVVVPLNAHWSDVGSWSAL
                     WEISRKDKSGNAIRGDVLIHDSSDSYLYSQHRLIGVVGVKDLVVVETKDAILVAHKDK
                     VQQVKNIVEKLKVNNRTEYLQHREIFRPWGSHDSIAEGSRFEVKHVVIHPGHKTAKQI
                     HYHRTEHWIVVSGTAKVHYEDEIFLVSENESTYIPIGVPHFIENPGKIPLEIIEVRSG
                     VYLDEDDVVRI"
     CDS             complement(3828..4283)
                     /codon_start=1
                     /transl_table=11
                     /gene="gmm"
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="AKI84597.1"
                     /translation="MNKRLETELFKSIVEHTPLISIDLIIRNEDGKALLGQRLNRPAQ
                     NYWFVPGGRILKDESFENAFKRVTFEELGVQISINEAKFLGIYEHFYSDNFSGTCFST
                     HYVVHGYEISLMPHQINYPTLQHSTYNWFDIAELLANSSVHQYTKNYFK"
     gene            complement(4286..5251)
                     /gene="fcl"
     CDS             complement(4286..5251)
                     /gene="fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="AKI84598.1"
                     /translation="MTKKRIFVAGHRGMVGSAICRQLLARNDVELIVKTHKELDLTVQ
                     KDVECFFEQERIDQVYLAAAKVGGIHANNTFPAEFIYQNLLIESNIIHSSYKAGIKKL
                     LFLGSSCIYPKFAEQPMKESALLTGTLEPTNEPYAISKIAGIKLCESYNRQYGCDYRS
                     VMPTNLYGMNDNFHPNNSHVIPALMRRFHEAKELGLNEVVVWGTGTPKREFLYVDDMA
                     AASVYVMELDDEIYKKNTQPMLSHINVGTGVDCSIREMAETMALVVGYDGKIVFDITK
                     PDGSPRKLMDVTRLENLGWKYRYNLKQGLELTYKWFINNLDSFRS"
     gene            complement(5255..6376)
                     /gene="gmd"
     CDS             complement(5255..6376)
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="AKI84599.1"
                     /translation="MCKKALITGITGQDGSYLAEFLLKKGYEVHGIKRRSSLFNTDRI
                     DHIYQDPHHRNLKLQLHYGDLTDTSNLTRILSEVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEVPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLYL
                     GNMDSLRDWGHAKDYVRMQWMMLQQDQPEDFVIATGKQISVREFVRMSAKEVGLELEF
                     SGTGVDEIAFVVNKTSDCAVGVNIGDVIVRVDPRYFRPAEVETLLGDPTKAKNVLGWE
                     PEITVEEMCAEMVASDLEKAKQHALLKSHGFDVAVSLER"
     CDS             complement(6387..7394)
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="glycosyltransferase"
                     /protein_id="AKI84600.1"
                     /translation="MKILYDGIVKSLQSYGGITVYFEEIIKRLKNDEYKFIQFNDKNE
                     NHNDDLHRPCRFLERYRCFEALKSDSDYSIFHSTYYRVPKDGEYAVVTTVHDFTYELY
                     MKGLRRNVHSLQKRWAVKNSDAIICVSNNTAKDLINFFKVDEKKIHVIHNGVSEDYRH
                     LNLEKENFVLFVGARSGYKNFELAVKAVSKLFDTNLKIVGGGGLNKAEMKLLSEYLPE
                     RFQYLGKVSNEKLNILYNSAYCLIYPSEYEGFGIPLLEAMRTGCPVIAVNRSSIPEVV
                     GDSAILVEEVSIESLFSALLSVEANANELKNKGLAQSNKFSWGKCFSETYNLYKSLSK
                     F"
     CDS             complement(7463..8479)
                     /codon_start=1
                     /transl_table=11
                     /gene="galE"
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AKI84601.1"
                     /translation="MSILVTGGAGYIGSHAILTLLQNGYDVISLDNYCNSSPKSLERV
                     EEICLSKIKKLRGDIRDRHILKNIFSKYNVSTVIHFAGLKSVNESIKKPLEYYDTNLL
                     GTLILLDEMQKANISNLIFSSSATVYGNPHYVPISEKHPVGEVINPYGRSKFMVEHIL
                     RDFCNANSDANITILRYFNPVGAHETGLIGEDPLGTPNNLFPYVAQVAIGKLPYLNVF
                     GNKYMTKDGTGVRDYIHVVDLVEGHLAALKNLNRNRGLKVFNLGTGQGYSVLDIIDAF
                     KLETGRDIPFRIVSERNGDVAECWSDPSLANEQLSWVAKRTLNNMVEDAWRWQTMNPN
                     GYKE"
     CDS             complement(8500..9567)
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="glycosyltransferase"
                     /protein_id="AKI84602.1"
                     /translation="MSIGVFKLYKNKIGGTEKAVENLIDILTKNSLQVRCFSAINAQE
                     IQVREDNRKNYLFQDNTAYEESKLRFYIKYLFFLSAKSKDLELIISTDIATSSLLLLI
                     GFFRYNKNKIICWEHYPFDKNSIFWRKVFKFLLSLNKKAVVVCNSHKEKDKFSFCKRV
                     VIIPNSIKKQKYKRIHQGKETLDLIYIGRIVKDKGVQRLINAVINHPSTKKYVLHIVG
                     GGQELEYLKSKYKNNNQIHFHGPSLNTDVFLAKSDVFVSGSYFECFPVTILEAMSYGL
                     PVISMDISGGTSSVISSSGGGFVCQSQIEFQEKLTYFEDNVKRTEHGNAGFLFVNRHY
                     SEEIVSAQWMSLINRANSQEN"
     gene            complement(9569..10804)
                     /gene="wzy"
                     /locus_tag="PROKKA_00010"
     CDS             complement(9569..10804)
                     /gene="wzy"
                     /locus_tag="PROKKA_00010"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AKI84603.1"
                     /translation="MINRNIKISASFISIIYLPLALLLAMIEMINGARWSVYVCSFML
                     GVLAFLLIPYENWDLTRHYETYLYVSQTSLNKVFENSDNRYITINTVIYLFKSLGIKK
                     EFLPFFSTFLSYIFYFNIYVNYIKKYNPELSHRCIYLYILISVIPFFAIASSLRQYLA
                     FSIMLYMIVQYFTSENNRYKSQKIIFSAIISCCIHPSAILLLGVFFVSKLIRLNKTIL
                     YLIILVLILNHDGLISSLLFQLIRPLLESLGFYFPEYMDSVTIGISNSLLSTNEYILN
                     KIILPSVFFIFTFIYLILFNKKMTRQESQIKNFCSLLLLLICLLSLSPDLFYRFSIFW
                     VLLYSYIFFSYDINKISKLCKHLIFLILIAACSIINLAQANMGKDIIYVSWCDILYKP
                     SLFLFNKEVKSAEYIRVSQ"
     CDS             complement(10801..11526)
                     /codon_start=1
                     /transl_table=11
                     /gene="orf11"
                     /product="glycosyltransferase"
                     /protein_id="AKI84604.1"
                     /translation="MNKPTLTIVTVVYNGEMYIEDTIKSVLSQNCNDDIEYIIIDGAS
                     TDNTLNIVNSYKNEISYIISENDNGIYDAMNKGVLKASGNYIGFINADDFYNKDVLCQ
                     VIKVMKENKYDVIYGNMSLVEADSLDVIQVRRPKDWKLHIDMNLSHPATFIKKDIHRE
                     NLFNTSYKIASDYDLLLKLKKQGYIFHYIDKNISSMRNAGLSAKNVKLALEEARQIRK
                     KRNGKLYAALADIYLFMRKVIKR"
     gene            complement(11535..12770)
                     /gene="wzx"
     CDS             complement(11535..12770)
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AKI84605.1"
                     /translation="MLKNIIYLLSVQGGNYIFPLVTLPYLVRILEPTGYGIYGYSFAV
                     VQYFILFIDYGFNYSAPKIISISRENTDSISKVFWNVTFIKIIAASLGLLIIYLISEL
                     NIIDYQIKFIILAYISVVGNLIYPVWFFQGLEKMLGIAISMFSARLLTLCLTFLLVKS
                     EKDLGIAITIQSSITVIAGIISTYFLFSLKKIIWVPPSLSKMKELIYDGWHYFISSAA
                     ISLYTTSSTIILGALSGTTAVGYFIAADKIRLALQGVIGPITQAVFPRVTYIMKDNHD
                     DGLLLARKVFRWQFSLMLVLSIFLYFFADDIILIMYGKTYHESLEILKVLAFTPAIVT
                     VSNYIAILVMIPIGMKKEFTKIITTSGLIGLLIIIYLTNEYSGVGTAIGVISTEFIIM
                     ARFYFLIKKTNKLNNLNLL"
BASE COUNT     4112 a   2545 c   2011 g   4213 t
ORIGIN
        1 catcactgcc ataccgacna cgccgatctg ttgctttgac attgtttact cctgtcaggg
       61 ggtggtcacc gccgagtgtg cggcttaaat gtggtataga tgttaactca ctcatagaga
      121 tggtgaatag cattaccttt gctataccac actgaatgtc tcaccttttc cgtcatttaa
      181 aacttattta tatgaaatta ctgaattact tgttcagtaa ctcaaggata agttttgtct
      241 tttcttccat cagcggcaca tcaccgtgag attccacatt caaccgcacc accggttcgg
      301 tgttggaggt gcgcaggtta aagcgccaat cggcaaaggt catgctgatg ccatcggtgc
      361 gatccaccgc cagcgcctca cgggtaaagt gctgttcgac gcggttaatc gcctcaacgg
      421 ggtgcgccag tttgctgttg atctcaccgc ttgccggaaa cgccgccatc cggtcgcgca
      481 ccagttcgcc cagcgttttt cctttcagac acaccagttc ggctaccagc agccacggga
      541 tcatgccgct gtcgcagtaa gcgaaatcac ggaagtaatg gtgggcgctc atttcgccac
      601 cgtagatggc gtcttccttg cgcatacgtt ctttaataaa ggcgtgtccg gttttcgaca
      661 ttaccggggt gccgcctgcg gcagtcacca catcaacggt attccaggag agacgcggat
      721 cgtggatgat cttcgcgccg ggattttttt cgaggaacgc ttctgccagc aggccgacaa
      781 tgtagtagcc ctcgataaac tgcccttttt cgtcaaacag aaaacagcgg tcaaaatcgc
      841 catcaaaggc aatgcccata tccgcgccgt gtttgatgac cgcattacgg gtgtcgtcac
      901 ggcattccgg cagcagcggg ttaggaatac cgttggggaa attgccgtcc ggcgtattgt
      961 gcactttgat gaattccact ggtgcgccga gggctttaaa tcgggcttca atggcgtcca
     1021 ccaccggacc cgctgcgccg ttcccggagt taatcaccag cttgagcggc gtgaggtttt
     1081 tgacgttgat ataaccgaac aggtgatcaa cgtaagcgtc acgcagattg atttgctgat
     1141 agcgaccgcg tttggtttca tcgacgggag gaaagtcgtt ggcttcagcc agacgctgga
     1201 catcgcgcag tccggtatca ccgctgatcg ggcgagcccc ttcgcgcacg agtttcatgc
     1261 cgttgtaatc catcgggtta tggctggcgg taacttcgat gccgccatcc acgccgagat
     1321 ggaacgtggc gaaatagatc tcttcggtgc cggacatgcc aatatccagc acatcgacgc
     1381 ccgcatcctg taaacctttc gccagcgcca gttttaaggt ttcgctggtg aggcggacat
     1441 caccgcctaa cacaatggtt ttcggtttga gaaattcgcc ataggcgcga ccaatgcgcc
     1501 acgcaatatc ttcattcagt tcttcgccta atttcccgcg aatatcatag gctttaaagc
     1561 aggttaattt tttcatataa aaaccttacc aataataaaa ttatttatgc gcaaacaatt
     1621 gcattagttt tgagattttt ctgataatga tagcaatttt ccaatttaca ttatggtttt
     1681 tcataacttc tacaatttcc tttgtacttg atatcgcgtt atcaattctg gtactttccc
     1741 cacccaatct catctttaca aatacttgag gaacataaat aactttaata tcatcacgtt
     1801 tcaaaatcct ggccatctga tcatagtctg cagcaatcct ataggataaa tcaaaatatc
     1861 catcgtcttt atataattcc ctcctcatat agaaagatgg atgcggtggt gcccagccta
     1921 atgatagctt agaacgttta aaaggaccac tacgccagta tcgtttgatt atatcaattt
     1981 ggtttttttc gaaaaaaacc aaatcaccgt atacaacatc cgcttttgtc tcatggaaac
     2041 ggcttacaat agtttcaata atatcaggtc ttgctaaaag gtcatcagaa tgaacgaatc
     2101 caataatatc tcccgtagat aaatttatac ctttatttaa agcatcatag ataccgttat
     2161 cgctttctga tagacatttg gttactcttg ttgatacttc gttaattagt tttaacgtat
     2221 tatctgtaga accaccatca ataattatat actctatatc tttataggtt tgcaactcta
     2281 ttgactctaa agtatccctt aaagtctttt cactattata agtcactgta ataatactga
     2341 ctttcatttt aacatccaat aataatcata attaaataac gtcagtacag cattacgaat
     2401 aataatgttg cattagtatc cagcatcatt ttagattcta acaacatcat cttcatccaa
     2461 gtagacacct gaacgtacct caataatctc caaagggatc tttcctggat tctcaataaa
     2521 atgaggtaca cctattggta tataagttga ttcattttca gaaacaagaa atatttcatc
     2581 ttcataatgg acttttgctg ttccagaaac aacaatccaa tgctcagtac gatggtaatg
     2641 aatctgttta gcagttttat gtccaggatg aataacgaca tgtttaacct caaatcgaga
     2701 tccttccgct atagagtcat gactgcccca tggcctaaat atttcacgat gctgcagata
     2761 ttccgtacgg ttattaactt taagtttttc tacaatattt tttacctgtt gaactttatc
     2821 tttatgcgca acgagaatag catctttagt ttcaacaact actaaatctt taactcctac
     2881 tacaccaatt aatctatgtt gtgagtaaag ataactgtca gaagaatcat gaattaatac
     2941 atctcctcta attgcatttc cacttttatc ttttctgcta atttcccata atgcagacca
     3001 agaaccaaca tcgctccagt gggcatttaa tggaactacc acagcctctg ttgttttctc
     3061 cataacagcg taatcaatgg attcttcagg gcaacaacta aatgagtcat tatctatacg
     3121 gataaaatca agatcttgtg tagaggactt caatgcttgt tggcatattt ttagtatgtc
     3181 tggtctaaat ttattcaact catcaataaa cacagaagcc ctaaacatga atattccact
     3241 attccaaaga taatctccat cttcaagata ttttttggcc acatctagct caggcttttc
     3301 aacaaaagca gaaactttaa aaccatattc atttaaattt tctccggttt ttatgtagcc
     3361 atatccagtt tcgggtttgg ttggcacaat accaaaagtt accagcttac cttcctctgc
     3421 aaattctttt gcagccaata ttgacgatat aaagcgcttt tggtcttgta tttcatgatc
     3481 ggctgctagt acaagcagta ttggatcctc acctgatgaa attgctttaa gagcagcaag
     3541 cgcaactgcg ggggctgtat tacgacctat cggctctagt aaaatgccac tattcgctaa
     3601 atttacatta cgcatctgtt ctgcaacgat aaaacgatga acttcattac aaataaccag
     3661 agcaggacga tgttcaactc cttcaagcct tcttaaggtc tcttgtagca tagtcaaacg
     3721 actagttaac gataggaatt gcttaggata tagtgttcga gaaagtggcc acaatctagt
     3781 tccagaacca ccggccatta caacaggaag tagcatataa ataaccttca cttaaaataa
     3841 ttttttgtgt actgatgaac tgaggaatta gctaacaatt cggctatatc gaaccaattg
     3901 taagtactat gttgtagtgt tggatagtta atttgatgtg gcatcaaact aatctcatat
     3961 ccgtgtacga cataatgagt tgaaaaacaa gtcccagaaa aattgtcgct gtagaaatgt
     4021 tcataaatcc caagaaattt tgcttcatta atgctaattt gaacacccaa ttcttcaaaa
     4081 gtaactcttt tgaatgcgtt ctcgaaagac tcatctttaa gaatccgtcc ccccggcaca
     4141 aaccaataat tttgtgctgg acgattaagg cgctgaccaa gcagcgcctt accatcttcg
     4201 tttcttataa taaggtctat cgagattagt ggagtatgtt caactattga tttaaataac
     4261 tctgtttcta atctcttgtt caatattagc tcctaaatga gtcaagatta tttataaacc
     4321 atttatatgt taattctagt ccttgtttta aattataacg atatttccaa cccaaattct
     4381 caaggcgtgt aacatccata agtttacgag gcgaaccatc tggttttgtt atatcaaaaa
     4441 caatttttcc atcatagcca acaactaaag ccattgtttc tgccatttca cgtatggaac
     4501 aatcaacacc agtacctacg ttaatatgag ataacattgg ttgagtattt tttttataaa
     4561 tttcatcatc aagctccatc acatatactg atgcagctgc catatcatca acatataaaa
     4621 actcgcgttt tggagttccc gttccccaga caaccacttc atttaaacca agttcctttg
     4681 cttcatgaaa acgtcgcata agcgctggaa ttacatgtga gttattggga tgaaaattat
     4741 cattcatacc atataagtta gtaggcatta cactgcgata atcacatcca tattggcgat
     4801 tataagattc acatagcttt atacctgcta ttttggaaat agcataaggc tcattagtag
     4861 gctcaagagt tcccgtcaaa agtgcagatt ctttcattgg ctgttctgca aactttggat
     4921 agatacaact tgaccctaag aataatagtt tctttatgcc cgctttgtaa gatgagtgaa
     4981 taatattgct ttcaataaga aggttctgat aaataaattc agctggaaaa gtgttattag
     5041 catgaattcc tcctactttt gctgctgcaa ggtaaacctg atctattctc tcttgctcaa
     5101 aaaaacattc aacatccttt tgaactgtca aatcgagttc tttgtgagtt ttgacaatta
     5161 actcaacatc attacgtgct aataattgac gacaaatagc agaaccaacc atcccacgat
     5221 gtccagccac aaaaattcgc ttcttggtca tactttaacg ctccaaagag actgccacgt
     5281 caaaaccatg gcttttaaga agtgcatgct gtttagcttt ttctaaatca ctagccacca
     5341 tttctgcaca catttcttca actgtaatct ctggctccca acccaagaca ttttttgctt
     5401 ttgttggatc accaagaaga gtttcaactt ctgctggtcg aaaataacga gggtcaacgc
     5461 gaacaataac atcaccaata ttaacaccaa cggcgcaatc tgatgtttta tttacaacaa
     5521 atgcaatctc atcaacacca gtaccggaaa actcaagttc aagtccaact tctttagcag
     5581 acatacggac aaattcacgt acagatattt gtttgccggt ggcaataaca aagtcttctg
     5641 gctggtcttg ttgaagcatc atccattgca tacgaacata atctttcgca tgcccccaat
     5701 cacgtaaaga atccatgttg ccaagatata agcatttttc tattccctgc gaaatattag
     5761 ctatagcacg tgtgatttta cgtgttacaa acgtttcccc acgacgtggg gattcatgat
     5821 tgaacaaaat accgttacat gcatacattc cataagattc acgatagttt acagtgatcc
     5881 aatatgcata cagtttagct acggcataag gtgaacgagg atagaacggc gttgtctctt
     5941 tttgtggaac ttcctgaact aatccataca attcagaggt tgacgcctga taaaatcggg
     6001 tttttttttc taatcccaaa aaacgaatag cttccaataa tcgtaaagta cctatagcat
     6061 caacatcagc tgtatattct ggggattcga aggaaacagc tacgtgactc attgcgccaa
     6121 gattataaac ttcatcaggt tgaacttcag ataatatccg agtcaaatta gaagtatctg
     6181 ttaaatcacc ataatgaagt tgtaatttta aattacgatg gtgtgggtct tgatatatat
     6241 gatcaatacg gtcagtatta aacaaggaag atcgacgctt tattccatga acctcatagc
     6301 cttttttaag cagaaattct gccaagtagg aaccatcttg cccggtaatt cctgtaatta
     6361 aagctttctt acacataaat aacctctcaa aacttagata gtgatttata tagattatat
     6421 gtttcggaga aacacttccc ccatgaaaat ttattagatt gcgctaatcc tttatttttt
     6481 aattcattag cgttagcttc tacacttaat aaagctgaga ataaactttc aattgatact
     6541 tcttcgacta aaatcgcact atcccccaca acctcgggta ttgatgatct atttacggct
     6601 atcacaggac agccagttcg catagcctca agcaagggaa ttccaaaccc ttcatattct
     6661 gaaggataga ttaagcaata agccgaatta tataatatat ttagtttttc atttgatacc
     6721 ttccctaagt actggaatct ttctggaaga tattcagata gtaacttcat ttctgcttta
     6781 tttaaaccac ctccgccgac tattttaaga ttagtatcaa aaagtttaga aactgcttta
     6841 actgcaagct caaaattttt atatcctgaa cgagcaccga caaaaagcac aaaattttct
     6901 ttttctagat ttaagtgtct ataatcttcc gatacaccat tatgaataac atgtatcttt
     6961 ttttcatcaa ccttaaagaa attaattaaa tccttggctg tattattaga gacacaaatg
     7021 attgcatcgc tatttttcac agcccatctt ttttgtaagc tatgaacatt gcgccgaagt
     7081 cctttcatat acaattcata tgtgaaatcg tggaccgtgg tcacaaccgc atattcccca
     7141 tctttcggaa cccgataata tgtagaatga aatatactgt aatctgaatc actttttaat
     7201 gcttcaaaac atctatatcg ttctaaaaac cggcatggcc gatggagatc atcattgtga
     7261 ttttcatttt tatcattaaa ttgaatgaat ttgtattcat cattttttaa tctcttaatg
     7321 atttcttcaa agtatacagt gataccacca tatgattgta aacttttcac aatcccatca
     7381 tataatattt tcattttttg ttttcatata tacagataac aatacagtaa gcaatgaaat
     7441 ggatgattat aattgtttca atttactctt tatatccatt tggattcatt gtttgccatc
     7501 tccatgcatc ttccaccata ttattaagag tccttttagc aacccaactt agctgctcgt
     7561 ttgctaagga aggatcggac caacattcag ccacatctcc atttcgctca gaaacgatac
     7621 ggaaaggtat atcgcgtcct gtttccagtt taaaagcgtc gatgatatct aacacactat
     7681 aaccttgacc agtgcctaaa ttaaaaactt taagcccccg gtttctattg agatttttta
     7741 aagcggcaag atgcccctca actaaatcga ctacatggat ataatctctt acacctgttc
     7801 catcttttgt catatattta ttaccaaaaa catttagata gggtaatttt ccaattgcga
     7861 cttgcgcaac atagggaaac aggttattag gagttcctaa aggatcttct ccaattaatc
     7921 cagtctcatg agcacctaca ggattaaaat atcttagtat tgtgatattt gcgtcgctat
     7981 tcgcgttaca aaaatcacgc aatatatgtt ccaccataaa ctttgaacga ccgtacggat
     8041 tgatcacctc accgacaggg tgtttttcag atattggaac ataatgagga ttgccataga
     8101 cagtagcaga cgaactaaaa attagattag aaatattcgc tttctgcatt tcatcaagta
     8161 atattagagt ccccaaaaga tttgtgtcgt aatattctaa tggtttttta atggactcgt
     8221 taacagattt taaacccgca aaatgtatta cagtagaaac attatatttt gaaaagatat
     8281 tttttaatat atgtctatct ctaatatcac cacgtaattt ttttatctta gataagcaaa
     8341 tttcctctac tcgttctaga gattttggag aggaattgca ataattatct agcgatataa
     8401 catcatatcc attttgcaat aaagttaata tagcatgtga gccaatatag ccagccccac
     8461 cagtcacaag tatgctcata ctttaatagc tccaatacct taattttctt gactattggc
     8521 tcgattgatc aaactcatcc actgagcact aactatttct tctgaataat gtctattcac
     8581 gaataggaat cctgcatttc cgtgttcggt tcgtttaaca ttatcttcaa agtaagttag
     8641 tttttcctga aattcaattt gagattgaca cacaaatccg ccacccgatg aagaaatcac
     8701 gctacttgtc cctccagata tatccattga aattacaggt aatccgtagg acatagcttc
     8761 aagtattgta acaggaaaac attcaaagta tgaaccggat acaaaaacat cactttttgc
     8821 caaaaaaaca tctgtgttta aactcggccc atgaaagtgt atttggttgt tattcttata
     8881 tttagatttg agatactcca gctcttgacc acctcctaca atgtgtaaaa catacttttt
     8941 tgtagacggg tgatttatta ctgcattaat taacctttga actcctttat ctttaacaat
     9001 ccgaccaata taaatcaaat caagagtttc ttttccttga tgtatgcgct tatatttttg
     9061 ctttttgatt gaatttggta taattacaac ccttttacaa aaactaaact tatctttttc
     9121 cttgtgagaa ttacaaacta ctacagcttt tttattaaga gacaaaagaa atttaaatac
     9181 ttttctccaa aaaatactat tcttgtcaaa tggataatgt tcccagcaaa taattttatt
     9241 cttattatac cgaaaaaaac caatcaataa caataatgat gatgtagcta tatccgtgga
     9301 aattattaat tcaaggtctt tacttttggc actcaaaaaa aacaaatatt taatgtaaaa
     9361 ccttaatttg ctttcttcat atgcagtatt gtcttggaat aagtagtttt tccgattatc
     9421 ttctctaact tgtatctctt gtgcgtttat tgctgaaaaa catctcactt gtaggctgtt
     9481 tttagttaat atatcaatta gattttcaac tgctttctca gtgccaccta ttttattttt
     9541 atacagctta aagactccaa tggacatttt attgactcac tcttatatat tctgctgatt
     9601 taacttcttt attgaatagg aataatgagg gcttatacaa tatatcgcac caagacacat
     9661 aaataatatc tttacccata tttgcctgtg ccaaatttat gattgaacat gcggcaatga
     9721 gtattaagaa gatcagatgc ttacatagtt tgctaatttt attaatatcg taggagaaaa
     9781 atatataact atataataac acccagaaaa tggaaaatcg ataaaataaa tcaggcgata
     9841 acgataaaag gcatatcagc aaaagaagta aactgcaaaa atttttgatt tgtgactctt
     9901 gcctcgtcat ttttttatta aataaaatta aatagataaa agtaaaaata aaaaatactg
     9961 acggtaatat gatcttattc aagatatatt cattagtgct cagcaagcta ttgcttatgc
    10021 ctatggttac agagtccata tattcgggaa agtaaaaacc taagctttcc aataatggcc
    10081 taatcaattg aaatagcaaa gaagatatta atccatcatg gttaaggatt agaactaata
    10141 ttattaaata caatattgtt ttgttaagtc ttattaattt gctgacaaaa aaaacaccta
    10201 gcaatagtat tgcgctggga tgaatacaac atgaaataat tgcagaaaat ataatttttt
    10261 gtgacttata cctattattt tcactggtga aatactgtac aatcatataa agcataatac
    10321 taaatgcaag atattgccgc aatgaactag ctatagcaaa aaatgggatt acgcttatta
    10381 gaatatataa atatatacat ctatgagata attccggatt gtatttttta atgtaattaa
    10441 catatatatt aaaataaaaa atatatgata aaaatgttga aaaaaaaggt aaaaattctt
    10501 tttttattcc taaagattta aataaataaa taacagtatt tattgtaata tatctattgt
    10561 cactattttc aaaaactttg tttagtgatg tttgactaac atataaatat gtttcataat
    10621 gcctagtcaa atcccaattt tcataaggta ttaacaaaaa tgctagtacc cctagcataa
    10681 atgaacatac ataaacactc catctggccc cattaatcat ttcaatcatt gctaataata
    10741 aagcaagagg caaataaatt attgaaataa atgaagcact aatttttatg tttctattaa
    10801 tcatcttttt ataacttttc tcataaatag gtaaatatct gctaacgccg catataattt
    10861 cccgtttctt ttctttcgga tttgtctcgc ttcctccaat gctaatttta catttttagc
    10921 gctaaggccg gcatttctca tcgaagagat atttttatca atataatgaa agatataacc
    10981 ttgtttcttc aattttagca aaagatcata atcagaagca attttatagc tagtattaaa
    11041 taaattttct ctgtggatat cttttttaat aaatgtggcc ggatggctta aattcatatc
    11101 tatatgtagt ttccagtctt tcggcctccg gacctgaata acatccaagc tatcagcctc
    11161 taccaaagac atatttccat aaataacatc atatttattt tctttcatta cttttattac
    11221 ttgacataat acatctttat tataaaaatc atcagcgttt ataaatccta tataatttcc
    11281 agatgctttt aacacacctt tattcattgc atcatatata ccattatcat tttcagaaat
    11341 gatatatgaa atttcatttt tgtaagaatt aacaatattt agagtattat ccgttgatgc
    11401 accatcaatt ataatatatt caatgtcatc attgcagttt tgtgataaga cactttttat
    11461 agtatcttcg atatacattt cgccattgta cacaacggta actattgtta aagttggttt
    11521 attcattgtt agccctacaa taaatttaaa ttatttaatt tatttgtttt cttaatcaaa
    11581 aagtaaaatc tcgccattat tataaactca gttgaaataa ctcctattgc tgtaccaact
    11641 cctgagtatt cattagtcaa ataaataatt atcagtaggc ctattaatcc actggtagta
    11701 ataatttttg taaactcttt tttcatacca atagggatca ttactaaaat tgcaatataa
    11761 ttacttaccg taactattgc tggagtaaaa gctaaaacct tgagtatttc taaagattca
    11821 tgatacgttt ttccatacat gattaaaatt atatcgtctg caaaaaagta aagaaatata
    11881 gaaagtacta acatcagtga aaattgccat cgaaacactt tcctggctaa taatagtcca
    11941 tcgtcatgat tatccttcat tatataagtt actctaggaa aaacagcttg ggttatagga
    12001 ccaataacgc cttgtagtgc aagcctaatt ttatctgcag cgataaaata tcccactgca
    12061 gttgttcctg ataatgcacc taatattatt gttgaactag tagtataaag gcttattgct
    12121 gcagaggaaa tgaagtaatg ccatccatca taaattaact ctttcatttt ggataaggat
    12181 ggtggtaccc atattatttt tttaagagaa aataaaaaat atgtagatat tatacctgca
    12241 ataactgtta ttgatgattg tattgttatc gctataccaa gatccttttc acttttaacc
    12301 aataaaaatg ttaaacatag tgtaagtaag cgtgcggaaa acatacttat cgctattcct
    12361 aacatttttt ccagcccttg aaaaaaccat actggatata ttaaatttcc tacgacagat
    12421 atgtatgcaa gaatgataaa tttaatttgg taatcaatga tattcaattc cgaaattaaa
    12481 taaataatta acaaaccaag agatgctgca attattttga taaacgttac attccaaaaa
    12541 actttactta tggaatcagt attttccctt gatattgata tgattttggg cgctgagtag
    12601 ttaaatccgt aatcaataaa taatataaaa tattggacca cagcaaaact atacccatat
    12661 atcccgtatc ctgtaggttc aaggatcctg acaagataag gcagtgtgac caatggaaat
    12721 atataattgc caccttgaac gcttaataaa tagatgatgt tttttaacat aacgagccta
    12781 cgtacttaac aagtcttatt ttgcacgata gtttacaatt tgttaagtaa tatgggataa
    12841 acatttaatt cacgctaccg cccctggctc aacagctacc ag
//
