LOCUS       KJ739596               13140 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  UNVERIFIED: Escherichia coli strain Pur599 O-antigen gene cluster,
            complete sequence.
ACCESSION   KJ739596
VERSION     KJ739596.2
KEYWORDS    UNVERIFIED.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13140)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13140)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Swimley,M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
REFERENCE   3  (bases 1 to 13140)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Swimley,M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
  REMARK    Sequence update by submitter
COMMENT     GenBank staff is unable to verify sequence and/or annotation
            provided by the submitter.
            On Dec 18, 2014 this sequence version replaced KJ739596.1.
            
            ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: 50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Pur599"
                     /serotype="OX21:H14"
                     /db_xref="taxon:562"
     misc_feature    1..13140
                     /note="O-antigen gene cluster"
     gene            398..1837
                     /gene="manC"
     CDS             398..1837
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AIG56898.1"
                     /translation="MMNHEKIFPIIMAGGAGSRLWPLSRELYPKQFIRLKGELTMLQA
                     TLSRLNTLMCNDPIIICNEQHRFIVAEQVRQLNKRTENIILEPVGRNTAPAVALAALA
                     AKRSRPDCDPLMLVLAADHVIQNEDAFREAVKVAIPYAENGKLVTFGIVPDTPETGYG
                     YIRRGEALFSVDEFKAFAVKTFIEKPNLETAIDYVSSGEYYWNSGMFMFRAGRYLEEL
                     EKYRPDILSACEKSMAVVDLDLDFIRVDEEAFLCCPEESIDYAVMEHTADAVVVPMDA
                     GWSDVGSWSSLWEVSDKTREGNVCTGDVISLNSGNNLIFSDTSLIATVGVHNLIIVQT
                     KDAVLIADRAAVQDVKKVVEKIKTDGRHEHHIHREVYRPWGKYDSIDTGERYQVKRIT
                     VNPGEGMSVQMHHHRAEHWVVVAGTAKVTINGEAKLLGENESIYIPLGATHCLENPGK
                     IPLDLIEVRSGSYLEEDDIIRFQDRYGRV"
     gene            1944..3344
                     /gene="algC"
     CDS             1944..3344
                     /gene="algC"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="AIG56899.1"
                     /translation="MEKLTCFKAYDVRGKLGEELNEDIAWRIGRAYGEYFKPRTIVLG
                     GDVRLSSKALKLALAKGLQDAGVDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPV
                     DYNGMKIVREGARPISGDTGLRDVQRLAEANDFPAVNEAKRGSYKQINLQKEYIDHLL
                     GYVNVANFKPLTLVINSGNGAAGPVVDALEARFKALNVPIKFVKVNNTPDGNFPNGIP
                     NPLLPECRSDTSNAVIELGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KHPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVTEMLCLKGKSLGEMVRDRMTAYPASGEINSTLAHPAEAIAR
                     VEQHFAKEALEVDRTDGLSMSFDEWRFNLRSSNTEPVVRLNVESRSNIELMEIKTKEI
                     MALLDNNNSGGRAENV"
     gene            3337..4590
                     /gene="wzx"
     CDS             3337..4590
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AIG56900.1"
                     /translation="MFSQLIQQLSSFVARILVSLISLLLSVIIVRLYTKDIVGDFFLL
                     TSYSTVLAQVFLFGMAPVLNIMSSKGVQLNKIVIEIAKKNVVSMLCMFLCGLIILAFF
                     AKVDYSSLIVLSLLSGVLLIISELMKGQGMYILSQIFNGGLNNILFLILIVVFIPNKE
                     ETDLEDIIYFYALAYMCVIVGGSIYLRIKYKVSKSKGLIDDVITYSIILPIFISTVIV
                     YMFSQIDLWFVSRLFDNDVVAQYGLAIRLTAVLSFSTLSVRAIAAARIPKLMNDIQAL
                     QKDVSLSCLFSFLVSLVILSGIALFGKLFIGLVYGNSYELTWYVLLIFSFGQMINAAT
                     GPCDYLLSHTGHGRYLMIISLVSFLFLMTLLAIITIRFNSVFLFCGAVSFTICLQNLI
                     IMYVAFKKTGVLSLPLINFKRAYVS"
     gene            4587..5822
                     /gene="wzy"
     CDS             4587..5822
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AIG56901.1"
                     /translation="MTRKTNTAVLSFMMLYILTSLISVYQTLNQDLFPGELHRYVRTI
                     ADDEVVIITTLNIISFIICYITFLLFQKFRFRLNKNCHYELNDKRIHFIIFVVLISQL
                     IFLYYTGIGKVTVNVEQRSTSSFSAFFAILKPEPFIFFYFLYYRLKPGFTFIKNKIFI
                     FNMFLFIVFKLFQGWTSFLLILFFLEVHARVQFTKNRLRYVLFIPLIIIFFGGWVYQY
                     AYVLKNEIRGAKVESITYLQGVEHLASRLSMNPNSLGAYQNYSKVINLYQQEGLALKE
                     SKAFLRPITPGGLVDKNFRNINNNVMTSYTPDLNQFTSSDFGIVMYYSILFNASLPDF
                     VLSIIMTVLLLVIAKMFFDSISSIPGQCDILFFFVLFYTFYTVSLENVFGQNFIPYIF
                     SFVIYYFLGGIKKVHFVEN"
     gene            5840..6847
                     /gene="gumI"
     CDS             5840..6847
                     /gene="gumI"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AIG56902.1"
                     /translation="MIVKMWPLQKESTANQYTSLILHDVIEYKPHKWNLKNVLDMRFD
                     IFHMHWPETYLNLPKRYQQLIGTLCVVLFLVVCKLFSKKIVWTVHNFKPHENHNNIKI
                     SEFFRTFLVRMVDKFIFLTEVSKDEFLKIYNIDNSRLTVIHHPLYQVNECCHDISAAN
                     HSSINNKYLFFGLIRPYKNIELLMNEFIKYDSDSVLIIMGGCSNTLATELRNMKKKID
                     FKERIKFKFGYYNEQQLQQELNQCKGVIIPYSDIMNSGVLYRAITAGVNVLLPRIRYT
                     EEVVNSLKYQGAVFYDPPLDTNDIATFNGIPSVNHNNFDVDTYNTYIKNAHYELYQTI
                     I"
     CDS             6867..7970
                     /codon_start=1
                     /transl_table=11
                     /gene="orf6"
                     /product="glycosyltransferases"
                     /protein_id="AIG56903.1"
                     /translation="MKNILIVCDYFYPAFKAGGPIKSLGNIAKTFNDRNYITVLTYNQ
                     DIDGQIINKSGTEIFEKNIVVYYAQNLLNFVKLYLKLYKTADIIYLNSFFSSRTTFPA
                     LMLKKTSAKVLLAPRGELTLGALTLKPLKKKIYIKLFNIFANKNEIYFQFTSEEEKKE
                     SLISLKKGFSYTVIPNMHDPIPPYIYKHKKEGEIHISFISRISPKKNLKAVLLSLLNI
                     AQGDILLSIAGNIEDEKYWAECMLIINKLPQNVKVNYLGGIKPTQVIDLLKSSHLFFL
                     PTLNENYGHAIVESMINSNVVLISDQTPWSDVQWNGGYVAGYNDTDTFKKCITECMKF
                     DESQFNNKSRQVYDYARNALTKHEYKTSTLFDI"
     gene            8155..9186
                     /gene="capD"
     CDS             8155..9186
                     /gene="galE"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AIG56904.1"
                     /translation="MFKDKVLLITGGTGSFGNAVLKRFLDTNIKEIRIFSRDEKKQDD
                     MRKKYSNNKLKFYIGDVRDYSSVLNASRGVDFIYHAAALKQVPSCEFYPMEAVKTNII
                     GTDNVLEAAIANKVSRVVCLSTDKAVYPINAMGTSKAMMEKVIVAKSRNVPDEMVICA
                     TRYGNVMASRGSVIPLFVNQILTGKPITITDPNMTRFMMTLDDAVDLVVHAFKHGQNG
                     DIFVQKSPAATIEVLTHAILEIMKSADHTVNIIGTRHGEKLYEVLCSREEMLVAEDQG
                     NYYRIPCDKRDLNYEKYFEKGNKKVETIDDYNSHNTYRLNTQEMVSLLRKLTYINKIE
                     AGEKADPDE"
     gene            9179..10054
                     /gene="rmlD"
     CDS             9179..10054
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AIG56905.1"
                     /translation="MNKRILIVGANGMLGSSLLRYFTQYSRYDVLGTVRSNSAKAKLS
                     KQGFDNIISQVDVLEYETIENVISDWKPDFLFNCVGIIKQLDAAKNNILSLSINSLLP
                     HQLAKTCTRNETKLIHFSTDCVFSGKQGNYCEESFPDAYDLYGKSKQLGEVVYDGHLT
                     LRTSIIGHEISSQHSLIDWFLSQKKYIYGYSKAIFSGMPTVYVAEIIHDYILPNPNCC
                     GLRHLSVDPIDKFSLLKLVKKQYSHNIEIIESSDLEIDRSLDSFLLRQEVGFYPASWP
                     KLIEKMNDEYNKFFR"
     gene            10029..11183
                     /gene="wbpI"
     CDS             10029..11183
                     /gene="wbpI"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronate
                     2-epimerase"
                     /protein_id="AIG56906.1"
                     /translation="MNTINSSVKKMKVVTVVGTRPEIIRLSRVMAVLDQYTEHTIIHT
                     GQNYDYELNEVFFKELEIRKPDYFLNAAGSNPAETIGNIIIEADKIFDIISPEALLIL
                     GDTNSALVSIAAKRRKIPIFHMEAGNRCFDYRVPEEINRKIVDHIADINLTYSEIARE
                     YLLREGIPADQIIKTGSPMREVLNYYNGEINKSKALEKLNLTPNEYFLVSAHREENVD
                     SPEKLKIFINILNKISDKYDLPIIVSTHPRTRNRMDKLNVEISNNVYFMKPFGFLDYV
                     SLQKNARVVLSDSGTITEESSILNFPALNLREVHERPEGFEEAAVMFVGLDAVRIFQG
                     IEILDQQQRGPDNRDLYLVNDYSVDNVSLKVLRIIMSYTNFVNQKVWKKQ"
     CDS             11183..12376
                     /codon_start=1
                     /transl_table=11
                     /gene="orf10"
                     /product="glycosyltransferase"
                     /protein_id="AIG56907.1"
                     /translation="MRIALICDDYLPDSTRVSAKMMHELACGLLREGHEPVVFCPNGG
                     VGTELKVIYLDGICIYKYPNGQTKDISKIKRAFNETMLSFNAWHYLSSEIKRKKIDGV
                     IYYSPSIFFGAFVKKIKKYWKCKSYLILRDSFPQWLVDQRIIRRNGMLEKYFRFFEKK
                     NYTAANYIGVMSSKNKELFALQYPHYKNVDVLYNWADLNSTQDVSPSDILERLSLADK
                     VIFFYGGNIGHAQDMANLMRLAIGMRTINYVHFLFVGQGDEVELVKKTIAENALENCT
                     YLPAISQSEYKSILKVVHVGLFSLAKNHSVHNFPGKLLGYMANKLPILGSVNEGNDLM
                     QVLIGAKAGYVHVNGYDAELLQSAIKLATEEKLRNELGENGHSLLEKTFSTQSAVENI
                     LLRLK"
     CDS             12399..12896
                     /codon_start=1
                     /transl_table=11
                     /gene="orf11"
                     /product="conserved LPS biosynthetic protein"
                     /protein_id="AIG56908.1"
                     /translation="MSVTVFYKEQLNELSNLASNNPRLRAHLNLHKSYQDKIQRILIS
                     LKRGTYIPPHYHKYDHQWEHFQVIEGIVDLYIFNNSGTVLDIINLGDVNGALFAQIEP
                     YTIHTLVCRSPYASVIEIKEGPFVEAEAKIVPSWVYPEGCREYLRSDIVSMLETLKIN
                     EVYKL"
BASE COUNT     4131 a   2110 c   2637 g   4262 t
ORIGIN
        1 aggcatgcat gcagtgctct ggtagctgta aagccagggg cggtagcgtg tctgatgaat
       61 aaatcgattt gttcaatata gtaatctact agaaaaactt ttcatccctc ataattatcg
      121 atgtcaatat ttgaaatata attagcaacc aacagttgat taaaatagtt caatgtttct
      181 gcataataat tatattgatg gtaaaaaatt aaacataacg aaccctattg ttttatgcgt
      241 ttatttataa cgtaaggccc atttaatagc tctgtaaata atgtaattca cttaaatttt
      301 ctttgtttta ccaaataccc tttggtttaa ccgaatttta tatttaacct tatcttcaag
      361 gtatgagtta tagctctggg ccttagaatg agttaatatg atgaatcacg aaaaaatttt
      421 cccaataatc atggctggtg gggcaggtag tcgtctgtgg ccactgtccc gcgagcttta
      481 tccaaaacaa ttcattcgcc ttaaaggcga actcaccatg ttacaggcaa ctctaagccg
      541 tttgaatact ttgatgtgta atgatccgat tattatatgc aacgaacagc accgctttat
      601 tgttgcagag caggtgcgcc agctgaacaa acggaccgaa aatatcattt tggagcctgt
      661 cggtcgtaac accgctccgg cagttgccct cgcggcgctg gcggcaaaac gcagtcgccc
      721 ggactgtgat ccgttgatgc tggttctggc tgctgaccat gttattcaga acgaagatgc
      781 atttcgcgaa gcggtaaagg tggctatccc ttacgcggaa aacggcaagt tggtgacttt
      841 cggcatcgtg cctgatacgc cggaaacggg atatggctac attcgtcgtg gtgaggctct
      901 ttttagcgta gatgagttca aagcctttgc tgtaaaaaca tttatagaaa agcctaatct
      961 tgaaactgcg atcgattacg tctcaagtgg tgagtattac tggaacagtg gtatgttcat
     1021 gttccgtgca ggtcgctacc tggaagaact tgaaaaatac cgtccggata tcttaagtgc
     1081 ctgcgaaaaa tcgatggcag tggttgatct tgatctcgat tttatccgcg tggatgaaga
     1141 agcctttctc tgctgtccgg aagagtccat tgactatgca gtgatggaac atacggcgga
     1201 tgccgtggtg gtgccaatgg atgcgggctg gagtgatgtc ggttcatggt cttctttatg
     1261 ggaagttagc gataaaactc gtgagggaaa tgtttgcact ggagatgtga tttcattgaa
     1321 ctctggcaat aatcttattt tctccgatac aagcttaatt gccacagttg gtgtgcataa
     1381 tctaattata gtacagacta aagacgcggt actgattgct gaccgtgccg ctgtgcaaga
     1441 cgttaaaaaa gtggtggaga agatcaagac tgatggtcgc catgaacacc atattcatcg
     1501 tgaagtctac cgtccatggg gtaaatatga ctccatcgac accggtgagc gttaccaggt
     1561 gaaacgcatc acagtgaatc cgggtgaagg aatgtcggtg cagatgcacc atcaccgtgc
     1621 cgaacactgg gtcgtggtgg ctggtaccgc caaagtgacc atcaatggtg aagcgaagct
     1681 gctcggtgaa aacgagtcca tttatattcc gctgggtgcg acgcattgcc tggaaaaccc
     1741 ggggaaaatt ccgctcgact taattgaggt gcgctctggc tcctatctgg aagaggacga
     1801 catcatccgc ttccaggatc gctacgggcg ggtgtagcac tccgcatcat gcccagtggc
     1861 gctgagcttg ctggatctac gaatgaaaaa atagatttga ccattcaagg gtcaggacta
     1921 attttgccta aaaaggagcc atcatggaaa aattaacctg ttttaaagct tacgatgttc
     1981 gcggcaagct aggtgaggag ctgaatgaag acattgcgtg gcgcatcggc cgcgcgtatg
     2041 gcgaatattt taagccacga actatcgtct taggcggcga tgtgcgtctg tccagcaaag
     2101 ccctgaagct ggcgttggcg aaaggactgc aggacgctgg tgttgatgtg cttgatatcg
     2161 gcctttccgg taccgaagag atctattttg caactttcca tttgggcgtg gacggtggta
     2221 ttgaagtgac agctagccat aacccggtgg actataacgg tatgaagatt gtgcgcgaag
     2281 gtgcacgtcc tatcagcggt gataccggcc tgcgcgatgt gcagcgcttg gctgaggcca
     2341 acgacttccc ggcggtgaat gaagccaaac gcggtagtta taagcaaatc aacttgcaaa
     2401 aagagtacat cgaccacttg ctaggctatg tcaacgtggc aaacttcaaa ccactcactc
     2461 ttgtaatcaa ttccggaaat ggagcggcag gaccggttgt cgatgcactt gaagctcgct
     2521 ttaaggcgct gaacgtgcct ataaaattcg ttaaagtgaa caatacgcct gacggcaact
     2581 tcccgaacgg tattcctaac ccgttgttgc cagagtgccg ttctgataca agcaatgcgg
     2641 tgattgagct cggtgcagac atgggaattg cctttgacgg tgatttcgac cgctgcttcc
     2701 tgttcgacga aaaaggacag tttatcgagg gttactatat agttggtctg ctggcggaag
     2761 cctttcttga aaaacaccct ggtgcaaaaa tcatccatga tccgcgtctc tcctggaaca
     2821 ctgttgatgt ggtgactgcc gcaggtggca cgccggtaat gtcgaaaaca ggacacgcct
     2881 ttattaaaga acgtatgcgc aaggaagacg ccatctacgg tggcgaaatg agcgcgcacc
     2941 attacttccg tgatttcgct tactgtgata gcggcatgat cccatggttg ctggtgacgg
     3001 agatgctgtg tctgaagggc aagtctctgg gcgagatggt tcgcgaccgc atgactgcct
     3061 atcctgcaag cggtgaaatc aacagcacct tggcacaccc cgctgaggcg atagcgcggg
     3121 tggagcagca ctttgcgaaa gaggcactgg aggtggatcg caccgatggt cttagcatgt
     3181 cgtttgatga atggcgcttc aatctgcgtt cgtcgaatac cgagccggtg gtacgtttga
     3241 acgtcgaatc ccggtctaat atcgagctaa tggaaataaa aactaaagag ataatggctc
     3301 tattagataa taataattca gggggccgag ctgagaatgt ttagtcagct aattcagcag
     3361 ttgagctcgt ttgtagcgag gatattagta tcattaattt ctcttcttct cagcgtgata
     3421 attgtacggt tatatacgaa agatatagtt ggtgattttt ttttgctaac cagttattcg
     3481 actgttttgg cccaagtgtt cttgtttgga atggcacctg ttttaaacat tatgtcatcc
     3541 aagggggttc agttaaataa aatagtaata gagatagcca agaaaaatgt agtatctatg
     3601 ctatgtatgt ttttatgtgg cttaattata ctggcttttt ttgctaaagt tgattattcg
     3661 agtttaattg tgctttcact tctttcagga gttctattaa ttattagtga actaatgaag
     3721 ggacaaggta tgtatattct ctcccaaatc ttcaatggag gtttgaataa cattctcttt
     3781 cttattctta tagtcgtttt tataccaaat aaagaagaaa cagatctcga ggatattatt
     3841 tatttttatg cgttagcata tatgtgtgtt attgtaggtg gttctatata cttgcgcatt
     3901 aaatataagg taagtaagag taaaggatta attgatgatg ttattacgta ttcaattatt
     3961 ttaccgatat tcatatcaac tgttattgta tatatgttct cacaaataga cctgtggttt
     4021 gtatctcgtt tatttgataa tgatgtagtt gctcagtatg gattagcaat tagattgacg
     4081 gcagtattga gtttttcgac tttatcagtt agagctatag cagcagcaag aatcccaaaa
     4141 ttaatgaacg acattcaggc gttgcaaaaa gatgtatccc tcagttgttt attttcattt
     4201 ttagtgagtt tagtgatttt aagtgggata gcattatttg gcaaattatt tattggcctt
     4261 gtttatggca acagctatga attaacttgg tatgttctac taattttctc atttggacag
     4321 atgattaatg ctgcaactgg tccctgcgat tatttactaa gtcatacagg gcatggaaga
     4381 taccttatga taatctcact tgtttcattc ttatttttaa tgactctatt ggcaataata
     4441 acaataagat tcaacagtgt gtttttattc tgtggcgctg tatcatttac aatctgcttg
     4501 caaaatctga ttataatgta cgtagcattt aaaaaaacgg gtgtactttc actaccctta
     4561 ataaatttta aaagagcata tgtatcatga caaggaaaac gaatactgca gttttatcat
     4621 tcatgatgtt atatattctt actagtctta tttcagttta tcaaacattg aatcaagatt
     4681 tatttcctgg tgaacttcat agatatgtaa gaactattgc tgatgatgag gttgtgatta
     4741 taacaactct gaatattata tccttcataa tatgttatat aacatttttg ttatttcaaa
     4801 aatttaggtt caggctgaac aaaaactgtc attatgaatt gaatgataag cgtatccatt
     4861 tcataatttt cgtagtgtta atatctcaac ttatttttct ctactacaca ggtattggta
     4921 aagttactgt taatgtcgaa cagcgtagta cttcatcatt tagcgctttt tttgcaatct
     4981 tgaagccaga accttttatt tttttctatt ttttatatta cagattgaaa cccggattta
     5041 cttttattaa aaataagatt tttattttta atatgttttt gtttattgtt tttaaattat
     5101 ttcaagggtg gacaagtttt ttgttaatac ttttctttct tgaagttcat gccagagtac
     5161 aatttactaa aaacagattg agatatgtac tctttatacc tttaattatt attttttttg
     5221 gtggctgggt ttatcagtat gcttatgtat taaagaatga aattaggggg gcgaaagttg
     5281 aaagtattac gtacttgcag ggagtggaac atcttgcatc acgtttatcg atgaatccta
     5341 actcgcttgg ggcttatcaa aattattcta aagttataaa cttatatcag caagaaggtc
     5401 ttgctctcaa agaatcaaaa gcatttcttc gacctattac accaggtggt ttggtggata
     5461 aaaatttcag aaatataaat aataatgtaa tgacatcata tacaccggat ctaaatcaat
     5521 ttacgagttc agattttggc attgtaatgt actattcaat attgtttaat gcaagtctac
     5581 ctgattttgt attaagtata ataatgactg ttttactgtt agtaattgct aaaatgtttt
     5641 ttgattcaat tagtagtatt cctggacagt gcgatatttt attctttttt gtattgttct
     5701 atacctttta tactgttagt ttagaaaatg tttttgggca aaactttatt ccttatatct
     5761 ttagttttgt aatatattat tttttaggtg gaataaagaa agtacacttt gtagaaaatt
     5821 aatttgagat tgtgtgatta tgatagttaa aatgtggcct cttcaaaagg aatcaacggc
     5881 aaatcaatat acttcgttga ttctacatga tgtgattgaa tataaacctc acaaatggaa
     5941 tctgaaaaat gtgttggata tgaggttcga tattttccat atgcattggc ctgaaactta
     6001 tttaaactta cctaaacggt atcaacagtt aattgggact ctgtgtgttg tattgttttt
     6061 agttgtatgc aagctttttt ctaaaaaaat cgtatggacc gttcataatt tcaagcctca
     6121 tgaaaatcac aataatatta agatatcaga attttttcgt acattccttg ttcgaatggt
     6181 agataaattt atttttttaa cagaagttag caaagatgaa tttctgaaaa tttacaatat
     6241 tgataattca agattaactg tgatacatca cccactgtat caagtaaatg agtgttgcca
     6301 tgatatttca gcagctaatc attcatcaat taataataaa tatctttttt ttggattaat
     6361 taggccttat aaaaacatag agttattaat gaatgaattt attaaatatg attctgattc
     6421 ggtattgata attatgggag ggtgctccaa cacattagct actgaattaa ggaacatgaa
     6481 gaaaaaaatt gattttaaag aacgcatcaa atttaaattt gggtactata atgaacaaca
     6541 acttcagcaa gagctcaatc aatgtaaagg tgtaataata ccatactcag acataatgaa
     6601 ttctggtgtg ctatatcgtg caataactgc gggtgtaaat gtgttactgc ctcgtataag
     6661 atatacagaa gaagtggtca attctcttaa atatcaaggt gctgtatttt atgaccctcc
     6721 acttgatacg aatgatatag cgacgtttaa tggcataccc tcagtaaatc ataataattt
     6781 tgatgtggat acatataata catatataaa aaatgcacat tatgaacttt atcaaaccat
     6841 aatttaagac ttaaattaag cgtaaaatga agaatatatt aatagtttgt gattattttt
     6901 atcccgcttt caaagcggga gggcctatta aaagtctcgg aaatatagcc aaaactttca
     6961 atgacagaaa ttacataacc gtattgacct acaatcagga tattgatggt cagatcataa
     7021 ataaatctgg cactgaaatt tttgaaaaaa atattgtcgt ttattatgct caaaacctgc
     7081 tgaattttgt taaattgtat ttaaaacttt acaaaacagc tgatataatc tatttaaata
     7141 gttttttttc gagtagaact acgtttcctg cattaatgct taagaagact agcgcgaagg
     7201 ttttgctggc ccctcgtggt gaactaacat taggtgcatt gacgttaaaa ccattaaaaa
     7261 agaaaatata tataaaatta tttaatattt tcgctaacaa aaatgaaatt tattttcaat
     7321 ttacatctga agaagaaaag aaggaatcat tgatatcctt aaaaaaagga ttttcatata
     7381 ctgtcattcc taatatgcat gatccgattc caccttatat atataagcat aagaaggaag
     7441 gtgaaatcca tatttcattt atttcgcgta tttcccccaa aaaaaatctc aaagcagtgc
     7501 tattgtctct tttaaatata gcccaaggcg acatactcct tagtattgca ggaaatatcg
     7561 aggatgaaaa atattgggcg gaatgtatgt tgataataaa taaattacca cagaatgtta
     7621 aagtaaatta tctcggtgga atcaagccta ctcaggtaat tgatttactt aagagctcgc
     7681 atttgttttt cttgccaaca ttaaatgaga actatgggca tgctattgtt gagtccatga
     7741 taaactcaaa tgttgtgcta atatccgatc aaacaccatg gtccgatgtg caatggaatg
     7801 gcggttatgt tgcaggttat aatgatactg atacattcaa gaagtgtata accgaatgta
     7861 tgaagttcga tgagtcgcaa tttaataata aatcacgaca agtctatgac tatgcacgaa
     7921 atgctttaac taaacatgaa tataaaacat caactttatt cgatatttag atgtaaattt
     7981 atgctttttc attttactta agtttctatg taaacagtta ttcccaatta acggtgtagt
     8041 attatttaac atcttttatt taaaattttc aatgtggtaa tgaattgagt tttattgctc
     8101 gcttttcttc atataaaaac actttacgtg tttgctcact ctagaggaat tagtatgttt
     8161 aaagataagg ttttacttat tacaggtggc acaggttcat ttggtaatgc tgtattaaag
     8221 cgttttttag atacgaatat caaagaaatt cgcatttttt cgagagatga gaaaaaacaa
     8281 gatgatatgc gaaagaaata tagtaacaat aaactcaagt tttatatcgg tgacgtaagg
     8341 gattattcga gtgtgttaaa tgcatctcga ggagttgatt ttatttatca tgcggctgca
     8401 ttaaaacagg ttccttcttg tgaattttat ccaatggaag cagtaaaaac taatattatt
     8461 ggcactgata atgttctgga agcagctatt gccaataaag tgtcaagagt agtgtgtttg
     8521 agtactgata aagcagtata cccaatcaat gctatgggaa catctaaagc aatgatggaa
     8581 aaggtcatag ttgctaaatc tcgtaatgtt cctgatgaaa tggttatatg tgcaacacgt
     8641 tatgggaatg ttatggcttc aagaggttct gttattcccc tttttgttaa tcagatatta
     8701 actggtaaac cgattacaat caccgatcca aatatgactc gtttcatgat gacattagat
     8761 gatgcagttg atttggttgt acatgctttt aaacacggac aaaatggaga tatttttgta
     8821 cagaaatctc ctgctgctac cattgaagtt ttgactcatg cgattctaga aattatgaaa
     8881 tcggctgatc acactgttaa tattatcggt acacgtcatg gcgaaaaact atatgaggtt
     8941 ttatgtagca gagaagagat gttggtagcc gaagatcaag gtaactatta tagaataccg
     9001 tgcgataagc gagatttgaa ttatgaaaaa tattttgaga aaggcaacaa aaaagttgaa
     9061 acaatagatg attataattc acataataca tatcgtttaa atactcaaga gatggtttca
     9121 ttactacgta aacttaccta tatcaataaa attgaagctg gtgaaaaggc tgatcctgat
     9181 gaataaaaga atattgattg ttggtgctaa tggcatgctg ggcagcagtc tgctacgata
     9241 ttttacccaa tatagtagat atgatgtttt gggtactgta cgtagtaaca gtgcaaaggc
     9301 aaaactatct aaacaaggtt ttgataatat catatctcaa gttgatgtgc ttgaatatga
     9361 aacaatcgag aatgttattt cagactggaa accagatttt ttatttaatt gtgtaggtat
     9421 tatcaaacag cttgacgctg cgaagaataa catactttca ttatcaatca attctttgtt
     9481 gccacaccaa cttgctaaaa catgtactcg taatgaaact aaactaatac atttttctac
     9541 tgattgcgta tttagtggta agcaaggtaa ttattgtgaa gaaagctttc cagatgctta
     9601 cgatctatat ggaaaatcaa agcaacttgg tgaagtggtt tatgacggtc atttaacttt
     9661 acggacttca attattgggc atgaaatctc aagccagcac agtttgatcg attggttttt
     9721 atctcaaaaa aaatatattt atggatactc aaaagctatt ttttctggaa tgccaacagt
     9781 atatgtagcc gagattattc acgactatat tcttccaaat ccaaactgtt gtgggctaag
     9841 acaccttagt gttgatccta ttgataaatt cagcttattg aagctagtaa aaaagcaata
     9901 ctctcacaat attgagatta tagaatcaag tgatttagag attgatcgta gtcttgattc
     9961 atttctatta cgccaagaag tggggtttta tccagcaagt tggcctaaac tgattgagaa
    10021 aatgaatgat gaatacaata aattcttccg ttaaaaaaat gaaagttgtt actgttgtgg
    10081 gtaccagacc ggagataatt cgtttatcga gagtaatggc tgttcttgat cagtacacag
    10141 aacataccat cattcacact ggacagaact atgattatga acttaatgag gttttcttca
    10201 aggagttaga gatcagaaaa ccagattact tccttaatgc agcaggttct aacccagctg
    10261 aaactattgg taatataatt attgaagcag acaaaatttt tgacataatt tctccagagg
    10321 ctcttttaat acttggggat acaaatagtg cactagtttc tattgcagct aaaagacgta
    10381 aaattccaat atttcatatg gaagcaggaa acagatgttt tgattatcga gttcctgagg
    10441 aaattaatcg aaagattgtg gatcatattg ccgatataaa ccttacttac agcgaaattg
    10501 ctcgtgagta cttgttgcgt gaagggattc ctgcagatca gattatcaaa acaggtagcc
    10561 caatgcgtga ggttttaaat tattataatg gagagataaa taaatctaaa gcactcgaaa
    10621 agttgaattt aactcctaat gagtattttc tggtcagcgc acatcgagaa gagaatgtag
    10681 attctcctga aaaattaaaa atttttatta acattcttaa taaaatatcg gataaatatg
    10741 atttgccaat aatagtatct acgcacccac gaacccgtaa tcgaatggat aagcttaatg
    10801 tagaaataag caataatgtt tattttatga aaccattcgg tttcttagat tatgtttctt
    10861 tgcaaaagaa tgctcgcgta gttctgtctg acagcggtac aataacggaa gaatcttcga
    10921 ttttaaattt tcccgctctt aatttacgtg aagttcatga acgtccggaa ggtttcgaag
    10981 aagctgccgt aatgttcgtt gggctggatg ctgttcgtat ttttcaaggt attgaaattt
    11041 tggatcaaca gcagagagga ccggataatc gagatttgta tttggtcaat gattattcgg
    11101 ttgacaatgt atcgctaaaa gtccttagaa tcataatgag ctatactaat tttgttaacc
    11161 aaaaggtttg gaaaaagcaa taatgcgaat agcgttaatc tgtgatgatt atctgcctga
    11221 tagcacacgt gttagtgcaa aaatgatgca tgaattggct tgtggacttt taagagaagg
    11281 ccatgagccg gttgtatttt gccctaatgg tggtgttggt actgaattaa aagtaattta
    11341 tttggatgga atatgtattt ataaatatcc aaatggacaa acaaaagata tatcaaaaat
    11401 taagcgtgca tttaacgaaa ctatgctttc attcaatgca tggcattacc tttcaagcga
    11461 aattaaacgt aaaaaaattg atggtgttat ttattattca ccatcaattt tctttggggc
    11521 ttttgtaaaa aaaattaaaa aatattggaa atgtaaatca tatctaattt tacgtgattc
    11581 atttccccaa tggttagttg accaaaggat aattcgtaga aatggtatgc ttgagaaata
    11641 ttttcgtttt tttgaaaaga agaattatac tgcagccaat tatattggtg taatgtcatc
    11701 taaaaataaa gaactttttg cattacagta tccccattac aaaaacgtag atgtcttata
    11761 taattgggca gatcttaata gcactcaaga tgtttcacca agtgatattt tagaaaggct
    11821 atcattggct gataaagtta tatttttcta tggaggaaat ataggtcatg ctcaagacat
    11881 ggcaaattta atgagattgg caataggtat gcgtactatt aattacgttc attttctttt
    11941 tgttggacaa ggggatgagg tcgaacttgt taaaaaaaca attgccgaaa atgcacttga
    12001 gaactgtaca tacttacctg caattagtca gtcggaatat aaatcaattt taaaggttgt
    12061 tcatgtaggg ttgtttagcc ttgcaaagaa tcattctgtg cataattttc cgggcaaact
    12121 tctaggatat atggcaaata aattaccaat tttaggtagt gtgaatgaag gtaatgatct
    12181 tatgcaagtg ctcattggcg caaaggcagg atatgtgcac gttaatggat atgatgcaga
    12241 attattgcaa tcagctataa aattagctac ggaagagaaa ttaaggaatg aattaggaga
    12301 aaatggtcat tcacttttag agaagacctt ttctacgcaa tctgctgtcg agaacatttt
    12361 gttacggctt aagtgaatta ttaaatgtag gaattgatat gtcggttact gtattttata
    12421 aagagcagtt aaatgaattg tcaaatcttg caagtaataa ccctcggcta agggctcatt
    12481 taaacttaca taagagttat caagataaga ttcagaggat tttaatttcc ttaaagaggg
    12541 gaacatacat accgccacat tatcataaat atgatcatca gtgggaacat tttcaagtga
    12601 tcgagggtat agtcgattta tatatattta ataattcagg tactgtctta gatattatta
    12661 atttgggaga tgtaaacggt gctttatttg cacaaataga accctatacc atacatacac
    12721 ttgtctgccg ttctccttat gcttctgtaa tcgaaattaa agaagggcct tttgttgagg
    12781 cagaggctaa aattgttccc tcttgggtgt atccggaagg ctgtagagag tacttacgtt
    12841 ctgatatcgt atcaatgctt gaaactctca agattaatga agtatataag ctttaactga
    12901 ttaaggataa tatggaatca atttaattaa atcaatcacc acctccctag gatgtggttt
    12961 aggaataaca attataaaat ttcttaatgt tgaaagaggc tgcagtttcc ctctattttt
    13021 ttattaggac ctgagttagc atcaaagtta cattcaagcc gcatacatcg cggtgaacac
    13081 cccctgacag gagtaaacaa tgtcaaagca acagatcggc gtcgtcggta tggcagtgat
//
