LOCUS       KP710594                9772 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain F6432 serotype OX25:H8 O antigen gene
            cluster, complete sequence.
ACCESSION   KP710594
VERSION     KP710594.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9772)
  AUTHORS   Baranzoni,G.M., Yan,X., Fratamico,P.M., Debroy,C., Tebbs,R.S.,
            Liu,Y., O'Connell,C.D., Swimley,M. and Matheny,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2015) Molecular Characterization of Foodborne
            Pathogens, United States Department of Agriculture, 600 E Mermaid
            LANE, WYNDMOOR, PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F6432"
                     /serotype="OX25:H8"
                     /db_xref="taxon:562"
     gene            47..1270
                     /gene="wzx"
     CDS             47..1270
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AJR19413.1"
                     /translation="MIWKNFGYLSLFNLITIAFPLLTYPYLTQYLGADYFSKSIYIQT
                     IISYFTVFVGFGINISGTKIIAAHRNDKNEIDKIFWQFFVVKLFLASISTMLLVLVVI
                     SFEDCFFYFLSFPLIYAELFFPQWYYQGMERMKNITIINLVSRVMVFLMIIFYVKGPS
                     DVYVYLIIFSFIPFIVNFFSFSYLTFSDKILFKKEYCKISELKGIIKYSLPIFCSTFL
                     SVIKDRTNIIIIGALIGAHQVIVYDFLIKVINVISGIYSSFTNAQFPYFVKNKDEEKY
                     KKYTLYILISSLIMVLVSIIIINIIKSIGTLAGISILNILFFDEFNIIFYWLIWLVPL
                     RALSYQIGMCKLIPEGLENKYTFTLLISTIIYMAGNGIVYLLKVDNLNVILFVLILSV
                     FFEVIYRLLVSKLKS"
     misc_feature    47..9631
                     /note="O antigen gene cluster"
     CDS             1276..2442
                     /codon_start=1
                     /transl_table=11
                     /gene="orf2"
                     /product="glycosyltransferase"
                     /protein_id="AJR19414.1"
                     /translation="MNKKLLFDLTSTQASPEAMFHGGAEYAKLVFHKAVELRYAEQMV
                     CFYNGKWELDSDIKRKCGQYAIELISIDQKKDIDLLIKREDINGFYSAIPYHYHDLNM
                     GDKKFIGTIHGLREIECPGDFYEYKYRKGIISVTKAIIRSLVIPKCVRVRRVKENISK
                     IFAYKNLKIITVSQHSKYSILANFPEIKSEQIIVIDSPYEFQAKKANDKNSLEKGNSY
                     FLLVSGNRWVKNSYRAIQALDELITEKRLIQHKVIITGCDGYKLLQKVKNRDSFLFLG
                     YVSSKELRHLFQHASYFIYPTLNEGYGYPPLMAFEFGVPVLASGNTSIFEVCKNAAIY
                     FDAHSISEIKTRILRLYSDSHLREKLILNGYEIIEALKEKGDRAEDIVNEIFKK"
     gene            2423..3529
                     /gene="pglJ"
     CDS             2423..3529
                     /gene="pglJ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AJR19415.1"
                     /translation="MKYLKNDVVIVIPQLGKGGGEKVACNLANAFSRRGINATLLVYK
                     NNSSNDYKRFIDDSVNVVYLDFDKKISRSPIKFIYKLIRNINTINPTTVIFCSGTVNC
                     IVSFFLWGLNKSIKKIARETNLPSLYSNILLRKLYGISYHKFDEIIVQSKEMYNDINA
                     ITKCKKKQIKLINNPLELDRIKEYREEYIDVPLFNKHAINIVTAGRLTYQKGYDLLIM
                     MVSNLVNLSKDIGFHILGAGELSDELRALVLKLNLADYIHFYGNVENPYPYYKKADAF
                     VSSSRWEGFPNAVIECLACGTPVIANHYPGGLTEIITNENGVIIDINDSKSFYEAIKY
                     VVNLEPQRIENTIKHLDSIEISKLIIKPEDVNDR"
     gene            3519..4568
                     /gene="wzy"
     CDS             3519..4568
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AJR19416.1"
                     /translation="MIDSTGIVYYVVLGFSAFVAFISDKAKSQMLSYYFAIISFLPIY
                     LLCALRYNVGSDYIAYQDIFYNIKYGYGSYVEPLYYSLNYLFSAFKNGWLGVFAISAF
                     ITFTPLFYIGCKEKILFWVVLLIFASGFLFRANNLIRQGIATAFFLLAIYWLHRGNNK
                     KFYTNIIVATLFHYTSIVFIASSLLRQKIKVHKNIYVLLATVLLIASFFINATDIIKR
                     IISLVPYYGEIYSIKLIDQSAGNGTNIVVYFWALVSIIMFYIRPNNIMALLFMIGSVS
                     SLYFLQLSLAFRFLSYLTDIKILLIGLYLKDFRVIKGYYAFTFVFVLMMLCLVFVNIT
                     NGISGSLPYRAFLSI"
     gene            4580..5617
                     /gene="cotSA"
     CDS             4580..5617
                     /gene="cotSA"
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein SA"
                     /protein_id="AJR19417.1"
                     /translation="MIYWVLGRRKNRNTGINKYSEGYIKNIRENIKVVYVPSVFEGWL
                     YPFGKYLYLPFYLFLKGFRRNKKDIVLPDESFSFLYYFLINWRHRYIIVHDYRDVEPK
                     TWKENVKAILMRFNYGMLFKYDKIITVSEYTREVIERKLKIPNKKINVIYNIIDFDET
                     NLISGCNKNKYGNKTIFLYVGSHESRKNTELLVRVFSQLPHLTLIMAGKIIDSSNNMK
                     INNLIKNCNNIYNLGEVDSDELQSLYYTANYFICVSSFEGFGRTPVEAQLYKLPVLST
                     WNTGLCEVLEKGSFYLIREPLNEVSIKTAISDFLNLSQEEINLFIKNGTRNAERFSSK
                     NLVKKMEKIIQ"
     gene            5614..6792
                     /gene="wfaV"
     CDS             5614..6792
                     /gene="wfaV"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AJR19418.1"
                     /translation="MRKILVLTPRFPYPVIGGDRLRIYKICEYLSKYFDLTLLSLCEK
                     KEEINMDVNDGVFKEIHRIYLPRFISYLNTIISVPTSTPLQVAYYKSYKYKKKLEELL
                     PKHDAVFAHLIRTGDYIKNKDTVKILEMTDAISLNYKRVRQNSITNFRSFVYSIEQKR
                     LEHYEKSMSEHFDLLSLISSIDKEFLFKDKMNVKIYGNGVDTNLLPFYKREIQEENVN
                     LIFIGNMHSLQNMDAVLWFAKCILPKINVKGKNVILKIIGRIKEADEIKLSKYSNVKI
                     IGAVNNVADASLDGHIGICPIRLGAGVQNKILEYMALGLPCITSSVGYEGIGASNKEN
                     ILIADTIDEYNHEINALVSDKKYYNFIAINARNFVESKYSWSAKLESMISDVTNLIEK
                     "
     gene            6794..8191
                     /gene="manC"
     CDS             6794..8191
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="AJR19419.1"
                     /translation="MILPVIMAGGSGSRLWPLSRTHYPKQFHCLVKETSLLIDTFKRL
                     DNLQHKEPLIICNEEHRFLVAEQLRSEKRNTSGIILEPQGRNTCPAIALAAFYASRHG
                     DDPYLLILAADHAIGDEDKFIDSISTALACAANDKLVTFGIVPTAPETGYGYIKKGNK
                     IADCVFKVDSFKEKPCKELAQSYLESGDFLWNSGMFLFKASIFLNELKIKEPEIYSQC
                     LEAIINSHSDSNFTYIDHAIFSQCKDVSVDYAIMEKTDKCAVVPMNANWSDVGSWSSL
                     WEISSKDSMLNHTHGDVIAIDSKRNYIRSESAITATLGVNDLIIVNTHDALLVANRES
                     VQDVKKIISELKKDNRRELSLFKNEYRPWGEIERIDKNDGYIVNRITISPGANISKQV
                     HYHRSEHWIVVSGTAKVTIEDDLFILTENESTFIRAGQMHSITNPGIVPLIIIEIQAG
                     SYISDADIYRIAEGN"
     gene            8192..9631
                     /gene="manB"
     CDS             8192..9631
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="AJR19420.1"
                     /translation="MENKSNKICDIIHNSNIAFGTSGARGLVVDFKQDVCTAFIQSFY
                     HLIKDKFSFSHVALAIDNRPSSYSIVQYCASALRVLNIPVIYYGVIPTPALALKAKND
                     SVPCIMITGSHIPFDRNGIKFYTPDGELTKKDESILLNCEEEIVCGSDFDLSVDNGAC
                     DFYIKRYSELYDDKPLSGKLIGIYEHSSAGRDLYSIVFENLGAKVVPLDRSDVFVPID
                     TEAVSDSDRGKAKNWCQEFKLDAIFSTDGDGDRPLVADENGIWLRGDILGLLCAEFLS
                     IKNLATPINCTSAIDIRNSFSKIVRTKIGSPYVIEALSDMSCKDGGGIAGFEANGGFI
                     LGSDIIYNNRTLQALPTRDALLPAIIVMVESFKTSTISQCVSNLPQRFTFSDRITDYP
                     SNKSRNLIFYAINSPSDFINKIGLRDFCVLSVNQLDGVRITASNGDIIHLRASGNAPE
                     LRCYAESDTNEKAEKYVKWILKSIEDNFS"
BASE COUNT     3365 a   1289 c   1756 g   3362 t
ORIGIN
        1 actggtagct gttaagccag gggcggtagc gtgtcgaatt attaatatga tctggaaaaa
       61 ttttggctac ctaagtttgt ttaatttgat aactattgca tttccgttat taacttaccc
      121 atatttgact caatatctag gtgctgatta tttctcaaaa tccatatata ttcaaacaat
      181 aataagttat tttactgtat ttgttggttt tggcataaat attagcggga caaaaataat
      241 tgctgctcac agaaatgata aaaatgaaat agataaaata ttttggcagt tttttgtggt
      301 aaaattattt ttagcctcaa tatccacaat gttattagta ttagttgtaa tctcttttga
      361 agattgtttt ttttactttc tctcattccc attaatttac gcagaattat tttttcctca
      421 atggtattat caggggatgg agagaatgaa gaatattacc attattaatc tagtatcaag
      481 agtaatggta ttcttaatga taatttttta tgtaaaagga cctagtgatg tatatgtata
      541 tctaataata ttttcattca ttccttttat cgttaatttt ttttcttttt catatctaac
      601 atttagcgat aaaattttat ttaagaaaga atattgtaaa attagcgagc taaaaggtat
      661 aataaaatat agtttgccaa tattttgcag cacattttta tctgtgataa aggatcgaac
      721 taatattatt attataggcg cactaattgg tgcgcaccaa gtaatcgtat acgacttttt
      781 aatcaaggtt attaatgtaa tatcaggtat atatagcagt tttacaaatg cacaatttcc
      841 gtattttgtg aaaaataaag atgaggaaaa atataagaaa tacacactgt atatattaat
      901 tagtagtttg attatggtat tggtatctat tataattata aatatcatta aatcaatagg
      961 tactttggca ggtatttcga ttctgaacat attgtttttt gatgagttta atattatttt
     1021 ttattggcta atctggttag ttccattacg tgctctttcc tatcaaatag gtatgtgtaa
     1081 gttaataccc gaaggtttgg aaaataaata cacatttact cttttgatat caacgattat
     1141 atatatggct gggaatggaa tagtgtattt attaaaagtt gataatttaa atgttatatt
     1201 gttcgtttta atattatccg tattttttga agtcatttat aggttgttag tgtccaagtt
     1261 aaaaagttga tttttatgaa taaaaaatta ttatttgatc ttacaagtac gcaagcttct
     1321 ccagaagcca tgtttcatgg cggagctgag tatgcaaaat tggtgtttca caaagcagtt
     1381 gagctacgct atgctgaaca gatggtgtgt ttttataatg ggaaatggga gctcgatagt
     1441 gatattaaaa ggaaatgtgg gcaatacgct atcgaactta taagtattga ccaaaaaaaa
     1501 gatattgact tactaattaa aagagaagat attaatggat tctattctgc aataccatat
     1561 cattatcatg acctaaatat gggtgataaa aaatttatag gtactattca tggattaaga
     1621 gaaattgaat gtccagggga tttttatgaa tacaaatata gaaagggaat tataagtgta
     1681 accaaagcaa ttatcaggtc gttagtaata cctaaatgcg taagggtgag aagagtaaag
     1741 gaaaatataa gcaagatttt tgcttataaa aatcttaaaa taataacagt atctcagcat
     1801 tcaaagtatt caatattagc taatttccct gaaataaaaa gtgaacaaat tattgttatt
     1861 gactcaccat atgaatttca agccaaaaaa gctaatgata aaaattcttt agagaaagga
     1921 aattcatatt ttttacttgt aagcggaaat cggtgggtta aaaactctta tcgagcaatc
     1981 caggctctag atgaattaat aacagaaaaa agattaatac aacataaagt aataattaca
     2041 ggttgtgatg gatataagtt acttcaaaaa gtcaaaaatc gtgattcttt ccttttcttg
     2101 ggctatgttt ccagtaagga gttgagacat ttgtttcaac atgcatcata ttttatttat
     2161 cctactctaa atgaagggta tggataccca ccattaatgg cctttgagtt tggggttcca
     2221 gttctagcat ccgggaatac tagcattttt gaagtctgta agaatgccgc aatttatttc
     2281 gacgcacact ctatatcaga gataaaaaca agaatactcc gtctttattc agattcacat
     2341 ttgagagaga aattgatact taatggttat gaaattattg aagccttaaa agaaaaagga
     2401 gatcgggccg aggatattgt caatgaaata tttaaaaaat gatgttgtga ttgttattcc
     2461 tcaactaggg aaaggtggtg gtgaaaaggt agcatgcaat ctcgcaaatg catttagccg
     2521 caggggaatc aatgcaacat tattagtata taaaaataat tctagtaatg attataaaag
     2581 atttatcgat gactcagtaa acgttgttta cttagatttt gataaaaaaa tcagtagatc
     2641 cccaattaaa ttcatttata agttaattcg taatattaat actataaatc caacaacggt
     2701 tatattttgt tctgggactg taaattgtat agtatcattc tttctttggg ggttgaataa
     2761 atcaataaaa aaaatagcca gggaaacaaa cctgccttct ttatactcta acatcctatt
     2821 aagaaaattg tatggtataa gttatcataa gtttgatgag attattgtac agagtaagga
     2881 aatgtataat gatattaatg cgataacgaa gtgcaaaaaa aaacaaataa aattaattaa
     2941 taatccttta gaattagatc gtattaaaga atatagagag gaatatattg acgttccgtt
     3001 gtttaataag catgcaatta acattgtaac agcaggaagg ctgacatatc aaaaaggata
     3061 tgacttattg ataatgatgg taagtaattt agtgaattta agtaaagata taggttttca
     3121 tatcttgggg gctggggaat tatctgatga gcttcgtgct ttagttttaa aactaaatct
     3181 agctgattac atacactttt atggtaatgt agaaaaccca tacccatatt ataaaaaggc
     3241 tgacgcgttt gtttcatctt cacgatggga aggatttcct aacgctgtaa ttgagtgctt
     3301 ggcatgtgga acacctgtaa ttgcaaacca ttaccctggt ggattgacag aaatcataac
     3361 taatgaaaat ggtgttatta tagatataaa tgactccaaa tcattttatg aagctattaa
     3421 atatgttgtt aatctagaac cacaacgtat agaaaataca attaaacacc ttgattcaat
     3481 tgaaatttct aaattaataa ttaagccgga agatgtaaat gatagatagc actggtattg
     3541 tatattatgt agtattaggc ttttcagcct ttgttgcatt tataagcgat aaagctaaat
     3601 ctcagatgtt atcgtattac tttgcgatca tcagctttct accaatatat ctactctgtg
     3661 ctttaagata caatgttgga agtgattata ttgcttatca agatattttt tataatatta
     3721 aatatggata tggatcatat gtagaacctc tttactattc attaaattat ttgttttctg
     3781 cttttaaaaa tggatggtta ggtgttttcg caatatcagc ctttattact tttactcctt
     3841 tattttatat tggatgtaaa gaaaaaatac ttttctgggt agtacttcta atatttgcat
     3901 ctggtttcct gtttagggca aataatttga ttagacaagg aatcgctacg gcattttttt
     3961 tactggcaat ctattggtta cacagaggga ataataaaaa gttttacaca aatattattg
     4021 ttgcaacttt atttcactac acttcaatag tttttattgc atcatcttta ttaagacaga
     4081 aaataaaagt acataaaaat atttatgtgc tgcttgcaac agtcttgtta atagcatcat
     4141 tctttataaa tgccacagac attataaaga gaattatttc tctggttccc tattatgggg
     4201 aaatatatag tattaagctt attgaccaaa gtgctggtaa tgggacaaat atcgttgttt
     4261 atttctgggc gttggtttct attataatgt tttatattag gccaaataat attatggcac
     4321 tattatttat gattggctca gtgtcatctt tgtatttttt acaattgtca ttagctttta
     4381 gattcttaag ctatttgacg gatataaaaa tcttattaat aggtctttat ctaaaagatt
     4441 ttcgtgtaat caaaggttat tatgctttta catttgtatt cgtgttaatg atgctttgtt
     4501 tggtttttgt taatataaca aatggcatat ctggttcgct tccttatcga gcttttcttt
     4561 cgatttaaga gatatataaa tgatatattg ggtattaggt cgccgcaaaa ataggaatac
     4621 gggtattaat aagtattctg aaggatatat aaagaatatt agggaaaata taaaggttgt
     4681 ttatgttcca tctgtttttg aaggatggct ctatccgttt ggtaaatatt tgtatttgcc
     4741 tttttatctt tttctgaagg gttttaggcg caataagaaa gatattgtgc ttcctgacga
     4801 aagtttttca tttttatatt actttttaat taattggcgg caccgttata taattgttca
     4861 cgactatagg gatgttgaac ctaaaacatg gaaagaaaat gttaaagcga tattgatgcg
     4921 ttttaattat ggcatgcttt ttaagtacga taaaataata actgtctctg agtatacgcg
     4981 agaagtgatt gaaagaaaac tgaaaatacc caataagaaa atcaatgtca tatataatat
     5041 aattgacttt gatgagacca atctaataag cggatgcaat aaaaataaat atggaaacaa
     5101 aactattttt ttatatgtag ggtcacatga aagcagaaaa aacactgagc ttttggttag
     5161 agtattttct caattgccgc atcttacttt aatcatggct ggaaaaatta ttgattcttc
     5221 aaacaatatg aaaataaata acctgataaa aaattgtaat aatatatata atcttggaga
     5281 ggtcgattcc gacgaactcc aatctcttta ttatacagct aattatttca tatgtgttag
     5341 tagttttgaa gggtttggtc gaactccggt tgaagcacag ttatataagc tgccagtcct
     5401 ttccacttgg aatacaggac tgtgtgaggt tttggagaaa ggctcctttt acttaatacg
     5461 agaaccatta aacgaagtgt caattaaaac ggctatatcc gattttctga atttatcgca
     5521 agaagagatt aacttattta ttaagaatgg tactcgtaat gctgaacgat tctcttcaaa
     5581 gaatttagta aaaaaaatgg aaaaaataat ccaatgagaa aaatattagt acttactcct
     5641 cgttttcctt atcctgtcat cggtggagat agactcagga tttataagat atgtgaatac
     5701 ctttctaagt attttgattt gacattatta tctctgtgtg aaaaaaaaga agaaataaac
     5761 atggatgtta atgatggtgt ttttaaagaa attcacagaa tctatttgcc aagatttata
     5821 tcttatctca atacaattat atctgttcca accagtaccc cgcttcaagt tgcatattat
     5881 aaatcatata agtataaaaa aaaattagaa gaacttttac caaagcatga tgctgtgttt
     5941 gcccatctta ttagaactgg ggattatatt aaaaataagg atacagttaa aatacttgaa
     6001 atgacagatg ctatctcttt aaattacaaa agagtacgac agaatagtat aactaatttt
     6061 cgtagttttg tttacagtat tgagcagaaa agactggaac attatgagaa aagtatgtct
     6121 gaacattttg atttgctttc gttgatttca tcaattgata aagaattcct ctttaaagat
     6181 aagatgaatg ttaaaattta tgggaatggt gtagatacaa acttattgcc attttataaa
     6241 agagaaatac aagaggaaaa tgttaatctc atttttattg ggaatatgca ttcacttcaa
     6301 aatatggatg cggtcctttg gttcgcaaag tgtattttgc caaaaataaa tgtaaaagga
     6361 aaaaatgtaa tattaaaaat aattggaaga ataaaagaag ctgatgaaat aaaattatca
     6421 aaatactcaa atgtaaaaat aattggcgct gtaaataatg tggcagatgc atctttagac
     6481 ggacatattg gtatttgtcc gatacgacta ggtgcaggag tgcaaaataa aatattagaa
     6541 tatatggcat taggtttacc gtgtattaca tcaagtgttg gatatgaggg aataggggct
     6601 tcaaataaag agaatatatt gatagctgat actatagatg aatataatca tgaaattaat
     6661 gcattggtta gtgataaaaa atactacaac tttatagcaa ttaatgcacg taatttcgtt
     6721 gaatctaaat attcatggtc agcaaaactt gaatcaatga tttcagatgt tactaatttg
     6781 atagagaaat aatatgatac ttcctgtaat tatggccggt ggttcaggtt caaggttatg
     6841 gcctttatct cggacacatt atccaaaaca gtttcattgt ttagttaaag agacaagttt
     6901 attaattgat acctttaaac gactagataa tcttcaacac aaagaacctt taattatttg
     6961 taatgaagaa catcggtttt tagttgcaga gcaattgagg tcagaaaaaa gaaataccag
     7021 tggaattatt ttagagcctc aaggtcggaa tacttgcccc gccattgccc ttgcagcttt
     7081 ttatgcttcg aggcatggtg atgatcccta tctattaata ttagccgcgg accatgctat
     7141 cggagatgaa gataagttta tcgattctat tagtacggct cttgcttgtg cagcaaatga
     7201 taaattagtt acttttggga ttgttcctac tgcaccagaa actgggtacg gttatataaa
     7261 aaaaggtaac aaaatcgctg actgtgtgtt taaagtagat tcttttaaag aaaaaccatg
     7321 taaagaactc gcacaatcct atttagaatc aggcgatttt ttatggaata gtggcatgtt
     7381 tcttttcaaa gcttctatat ttttaaatga gctaaaaatc aaggaaccgg aaatatactc
     7441 acaatgttta gaggctatta ttaattctca cagtgatagc aattttactt acatagatca
     7501 tgcgatattc agtcaatgca aagatgtttc agtagattac gcgataatgg agaaaacaga
     7561 caaatgcgct gtcgtgccaa tgaatgctaa ttggagcgat gttggttcat ggtcttcctt
     7621 atgggaaatt tcatcaaaag atagtatgct caaccatacg catggtgatg tgattgctat
     7681 tgatagtaag cgaaattata tacgctcaga atcagcaatt actgctactc taggtgttaa
     7741 tgatttaatt attgttaata cccatgatgc tttgctcgtt gcaaaccgag aaagtgtaca
     7801 agatgtaaaa aaaataatca gtgaactaaa aaaagataat agaagagagt tgtcactttt
     7861 taaaaatgag tatcggccat ggggtgagat agaaaggatt gataaaaatg acggttacat
     7921 tgttaataga ataactattt ctccgggggc taatatctca aaacaagttc attaccaccg
     7981 ttctgaacat tggattgtag tatccggtac cgcgaaagtt acaatcgagg atgatttgtt
     8041 tatcttgacg gaaaatgaat caacatttat tagagcaggt caaatgcatt ctataactaa
     8101 cccaggtatt gtcccactga taattattga gatacaggca ggtagttata ttagtgatgc
     8161 tgatatttat agaatagcag agggaaatta aatggaaaac aaatcaaata aaatatgtga
     8221 tataatacat aattcaaata ttgcatttgg aactagtggt gcaagaggtc tagttgttga
     8281 ttttaaacag gatgtttgca cagcttttat tcagtctttt taccatttaa taaaagataa
     8341 gtttagtttt tctcatgtgg ctttagctat tgataataga ccaagcagtt attctatagt
     8401 gcaatattgc gcttcagctt taagggtgtt gaatatacct gttatttatt acggcgttat
     8461 tcctacacct gctcttgctt taaaagctaa gaatgacagt gtgccgtgta taatgattac
     8521 cggaagccat attccctttg atcgcaatgg aatcaaattt tatacgccag atggtgagct
     8581 aactaaaaaa gatgaaagta tactccttaa ttgtgaggag gagattgttt gtggaagtga
     8641 ctttgacttg tcagtagaca atggtgcttg cgacttttat attaaaagat attcggaact
     8701 gtatgatgac aaaccattgt cagggaagtt gattggtatt tatgagcact ctagtgctgg
     8761 aagagattta tactcaattg tatttgaaaa tttaggtgct aaagtagttc cgctcgatag
     8821 aagtgacgta tttgttccaa tagatactga agcagtaagt gattctgatc ggggaaaagc
     8881 aaagaattgg tgccaggaat tcaaacttga tgctattttc tcaactgatg gtgatggtga
     8941 cagaccatta gttgcagatg aaaatgggat atggttacgt ggggatattc ttggattatt
     9001 atgtgctgag tttctttcaa tcaaaaacct tgcaactcca ataaattgta cttcagcaat
     9061 tgatataaga aattcttttt ctaaaattgt aagaactaaa attggatcgc cttatgttat
     9121 tgaggctctt tcagatatgt cttgcaaaga tggtgggggg atagcaggct ttgaagctaa
     9181 tggtggtttt attttaggta gtgatattat ttataataat agaacattgc aagctcttcc
     9241 aacaagagat gcccttttac cagctataat agttatggtt gagtcattta aaacttcaac
     9301 tatttctcag tgcgttagta atctgcctca aagatttact ttttctgata ggattacgga
     9361 ctatccatca aataaaagta ggaacttgat tttttatgcc ataaactccc cttcagattt
     9421 tattaataaa attgggctga gggatttttg tgtactatca gtaaatcaac tcgatggtgt
     9481 tagaataaca gcatctaatg gtgatataat tcatttgcgt gcatctggta atgcgcctga
     9541 attacggtgc tacgccgagt ctgatacaaa tgaaaaagct gaaaaatatg ttaagtggat
     9601 attgaaaagt atcgaagata atttctcata acccactctt attataaagc aatgcaatat
     9661 atcattttta attactgaaa tgaatatata tgccatatta ttactcagat ccccctgaca
     9721 ggagtaaaca atgtcaaagc aacagatcgg cgtcgtcggt atggcagtga tg
//
