LOCUS       KT207929               14068 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain 7026N serotype OX28:NM O-antigen gene
            cluster, complete sequence.
ACCESSION   KT207929
VERSION     KT207929.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14068)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14068)
  AUTHORS   Yan,X., Baranzoni,G.M., Fratamico,P.M., Debroy,C., Tebbs,R.S.,
            Liu,Y., O'Connell,C.D., Swimley,M. and Matheny,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUN-2015) USDA-ARS, MCFP, 600 East Mermaid Lane,
            Wyndmoor, PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50x
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="7026N"
                     /serotype="OX28:NM"
                     /db_xref="taxon:562"
     misc_feature    complement(1..14068)
                     /note="O-antigen gene cluster"
     CDS             complement(195..1052)
                     /codon_start=1
                     /transl_table=11
                     /gene="IS"
                     /product="transposase"
                     /protein_id="ALP13866.1"
                     /translation="MRDCHSSDDKDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHS
                     LVLGQIKTDEKSNEITAIPELLNMMDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAV
                     KGNQGRLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFE
                     WKGLKKLCVAVSFRSIIAEQKKEPKMTVRYYISSDDLTAEKFATAIRNHWHVENKLHW
                     RLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLA
                     SVLAGSGLS"
     CDS             complement(1200..1331)
                     /codon_start=1
                     /transl_table=11
                     /gene="H repeat"
                     /product="H repeat-associated protein"
                     /protein_id="ALP13867.1"
                     /translation="MELKKLMEHISIIPDYRQARKVEHKLSDILLLTICAVISGAEG"
     CDS             complement(1905..3011)
                     /note="maturase"
                     /codon_start=1
                     /transl_table=11
                     /gene="orf3"
                     /product="reverse transcriptase"
                     /protein_id="ALP13868.1"
                     /translation="MTLYQALCRNYGNRSSQCQERNTNNRNYEYESIDARYRGRAVRS
                     SEEAFVMNVERRGSVGQSASQPNCKQEEAAGEQTKPFQVSKLHVVEAYRLVKANAGAA
                     GVDNQTLKDFERDLKGNLYKIWNRLSSGSWMPPPVRAVEIPKKDGSKRLLGIPTVSDR
                     IAQMTVLVTFEPLVERYFLNDSYGYRHGKSALDAIAVTRKRCWQYDWCLEFDIKGLFD
                     NIPHDLLLRAVDKHCADKWVRLYIRRWLTAPVQMPDGTLRERNKGTPQGGVISPVLAN
                     LFLHYVFDKWLTLHYPEIPWCRYADDGLIHCGSKQQAEKLLNKLAKRFQECGLELHPE
                     KTKIVYCKNSERQANHETAQFDFLGYNVQSAKST"
     CDS             complement(3396..4601)
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="ALP13869.1"
                     /translation="MSIRLSSLYLMILFLVYINISYSAGSDILVFAMCILVMSVPFIH
                     LKQSDCIFIGFLTTYYFIMLIFVWEAYGEISYLTGAIIQFSSSIFLGVCTYRLLLISK
                     LCRNKNILKRILIIYFIFLVLELFDVTRNVSNYYRQVFFGVTSDIIAMEHVRDLQMTK
                     WFIRPNVFSKEASLAYLFFFVITCCYLLNSRSGIVLAILVITNLILFIIFRTPSAALT
                     LLMYFSIVFFNKNIYFPSLTKKSKFIFNVFFIMFVFIFLFMISDFLLERFSDIDFNDA
                     NRNNSITIRVVAPLHFMFRVLSEYPLFGIGLGNLDVFRSDNVVVGNNATFYLISFLGI
                     IGTITLFLALAIWLYKEKQFYFSDFIIIILFFICLTLQTGAILNFRLWFYFFSFVAYF
                     KTFKELEIN"
     CDS             complement(4606..5388)
                     /codon_start=1
                     /transl_table=11
                     /gene="orf5"
                     /product="glycosyltransferase"
                     /protein_id="ALP13870.1"
                     /translation="MPHVDDKPKISIIMPAYNAQYTIAESIESVLRQTYLDFELIIIN
                     DNSTDDTTRIIQAYQIQDSRVILINNFFDKGAAGARQSGICHSQGEFIAFLDSDDLWH
                     ESKLENTINYMIKKNVSMTYGDYAVFRHNRFVNTTYEVPEKLSYKDLLKYCPIGCLTV
                     VIRRNIIENIRFRNVPKEDYDFWLQILKNEHIAYKVPGCMAFYRVGRDTLSSNKIKEL
                     KKQFMLLRYIHKMPLIKVFFNLSFYAARGIAKNIRIKMSLTG"
     CDS             complement(5372..6496)
                     /codon_start=1
                     /transl_table=11
                     /gene="orf6"
                     /product="glycosyltransferase"
                     /protein_id="ALP13871.1"
                     /translation="MNKNKCYFISINSIFELTGGGIYARTILNALKYKYSEIVVINKK
                     IELTNESKKEYNGFIVQLEKTKIADIVSRIFISPSFLFFYITSIIAEIKKNKGECVDL
                     FFHNSRLGIVALILKLLFNVRVFFFIDNNESNLSKQQAVLANGFFEKIFRYYDYILIK
                     ISECFVKKISSHLFFITKNDANAFKLNDDVKYSIIPVCVDDLYEKEIINRKPACLFTA
                     SFTFSPNVLALKRLLKLAEYYPTIDFIVAGRGLSKYIYLNNLKNVKFICSPSEQYMRE
                     LFKTCKGYITLVEDGSGMKTKIAEALSAGMYIYSSWHSAIGYEAVIDSGCIYVYKDMI
                     ADFNTWYSNLSEYDPYLSIKAYRNNYTYSHAGDILNAACR"
     CDS             complement(6496..7494)
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="glycosyltransferase"
                     /protein_id="ALP13872.1"
                     /translation="MNDLDVSAVILNYNSITDTINCLVSLSQQTLKPREIVVVDNKST
                     DSSINVLWEWVKNNAVIINEEYGSIKHSNVDCKVSLFVFEKIDIYFIKSPVNGGYAFG
                     NNLGANYICKYGESKLILLANPDVLFSTDSIEKLRNEYISVQKKDKQVKIIGVQLVYL
                     DDPEKIQAIGGSFNKLWGVCKHVGRQQNVSDLDFIKESVRVDYPIGACLLIESECFIE
                     LGGLNEAYFLYYEELDLITRAAKKKIKFTITNSTTIYHKEGGSIGSSSKSFDAVSSIS
                     DKYLLQSRLLFIKRYYPQYLFTNLMVHIFYILKRLWVRDFVKTWNAIRALKVLVKS"
     CDS             complement(7487..8545)
                     /codon_start=1
                     /transl_table=11
                     /gene="orf8"
                     /product="glycosyltransferase"
                     /protein_id="ALP13873.1"
                     /translation="METLRIIVVHEYGALNHYTALKYFCDNNNYELKFHEFGITPQIK
                     AIIKKNRLKNLRRFFSNLCFLLRRLISNKRNEIIILGVAPLDLRLALLLWIFRNNKVY
                     IHNSWPEWQDGQFPKGNSCVAKKVWTYALKNNIAGFFAVSRKTYREFINYFCFDKYNS
                     EVVYHSLKQGWLMDEAEIILKEKKRHILFMGRLVKEKGLVNILKIAKACPRYTFHIIG
                     DGPDSYVLKNQKNIIIHGHLSELEKIKEIVDRCSFLLQPSISCKGWKEAFGLTVIEAM
                     SRGIPVIATNQPGPLEIIDNKQGFIFTEETYLSCAIEVIDTLDYETWKKMSLASYNRA
                     KDFTIDKLSEKWGNLLDE"
     CDS             complement(8533..9228)
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="O-acetyltransferase"
                     /protein_id="ALP13874.1"
                     /translation="MRVNKLSTKEKILSFILGFHKTLYLNFSCFSFWTAIKFPVLVAK
                     NVKLVGIGKIILDTEPAFGLIKIGFGDIGIFDGYNSKTIIKNDGTLIFRGKTNIGHGT
                     KININNGAKLIFGKDFIISGESSLICSSKIEFGINCLISWDVLIMDTDFHQVYSVLNN
                     GEYSESGVKEPINIGDDVWIGCRCLIMKGVNIANGCVISAGTKLHKKIVKERTVISGE
                     LNKIVRENILWKH"
     CDS             complement(9218..10492)
                     /codon_start=1
                     /transl_table=11
                     /gene="wzx"
                     /product="flippase"
                     /protein_id="ALP13875.1"
                     /translation="MQNKASRVKNYFHSKVIPFFLRGGGAALSFISTILVSRYLGAHD
                     SGIYYVSLNIISVLSVVANFGFNTMILKQVSDQYIDKSRESILISSSLYCALAIAFLF
                     FALIFLIANVNGEKDLYWTLLLMSPAIFFMACSNSFASYLQAKERFTPMVIIQSFAVP
                     AILIFCIWLLHFLNTIDYNKIIIISSIYLLSCILVFIYAICSAKAEGDFQFLKCSIKF
                     KHGTFKYAMINIIIIALSQGVVIINGYFLSYSELSKVIVAQKIALLFNFVTTTIVMLF
                     IPKMINSFKNNKKEFFKIARKCKKIAIITNALMAIVVYITADYIISIYGESFDGASVN
                     LKILVLFQLINAYCAVNGSILLYIDNENILLKIYVAALSISILIALTSPFLFGNSNVI
                     ACISLGLSVITVNVFSTIRLFFISPVKITNEG"
     gene            complement(10473..11036)
                     /gene="rmlC"
     CDS             complement(10473..11036)
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="ALP13876.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKIFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSTTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTAFYNKDYECGISWEDELINIKWPMC
                     ENPVLSEKDANLNKLRVEFIQNAKQSI"
     gene            complement(11044..11919)
                     /gene="rmlA"
     CDS             complement(11044..11919)
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase 1"
                     /protein_id="ALP13877.1"
                     /translation="MKTRKGIILAGGAGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTSQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKG"
     gene            complement(11977..12876)
                     /gene="rmlD"
     CDS             complement(11977..12876)
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="ALP13878.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLINATSVEAIAKAANEVGAWAIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFMTTAI"
     gene            complement(12876..13961)
                     /gene="rmlB"
     CDS             complement(12876..13961)
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="ALP13879.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIHKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
BASE COUNT     4427 a   3019 c   2153 g   4469 t
ORIGIN
        1 atcactgcca taccgacgac gccgatctgt tgctttgaca ttgtttactc ctgtcagggg
       61 gtgttcaccg cgatgtatgc gcgacttgaa tatgattgag atgttatctc aagttgcacg
      121 aaggtgtgta gtgctgaatt tcattaatgc aacgaatact tttcttcttc ctatacccca
      181 tcatcagggc aagattacga aagcccgctc cccgcaagga ctgacgccag gtagtttctg
      241 tccatggctg cttttcgcat cttacgtctt aaccctgcct tgaatacctt atcattcgtt
      301 aaaatattaa tagcgatgtg ccgtatccct gaaaataatt ctgctgcatt tcctcttctt
      361 attttgcagt cgtcttcatt cattaccacg tccagacgcc agtgcagctt attctccacg
      421 tgccagtggt ttcggattgc tgtggcaaac ttctctgcgg ttaaatcatc agaactgata
      481 taatatctga ccgtcatttt tggctctttc ttttgttctg ctattatgga ccgaaaggag
      541 actgccacgc ataatttctt cagccctttc cattcaaacg tgaaatcaat aagttcatca
      601 gggacatcgc aaacaatatg aagacggatt tcttctctgc cgtgactctt ttcactaatt
      661 gcgtaactgt catgctctgg attatttaat tctttcagcg gaaatttttc ctcaaaggct
      721 ttatttagcc gcccctggtt tccttttacc gcgaataaat aatcacctcc ctgtttttgt
      781 atcttctctg caatatcttt ctggcaaccc atcgcatcag ttgtgatgat ttttccttta
      841 atatccatca tgttaagaag ttcaggaata gctgtaatct cattagattt ctcatccgtc
      901 ttgatctgtc cgaggaccag actgtgcatt gttgagaacg cactaatgac atgaatagct
      961 cccctgcgac gactcttatc ataagagtgc cggagcgttt ttccatcaat tgcaatgacg
     1021 tctttatcat ctgaagaatg gcagtcacgc atccagttaa taaagcactc atgaaatttt
     1081 gcaggactga tacagggtac aactctggta atggtatcgt gaacaggaat accattttaa
     1141 aaatcaccat attgcttcaa aaaatcgaga tgtgtttcac caaaaccctc tatatcttct
     1201 caaccctctg caccagaaat aacggcacaa atagtcaaca gtagaatatc cgataattta
     1261 tgttccactt tcctggcttg tctgtaatcg gggataatag aaatatgttc catcaatttt
     1321 ttaagttcca ttttgttctc cttaattatg taaggagtat ttgatcatgt ataagcaata
     1381 aaaaacagct tcaggcaatg aggaatatct caatttttaa acataaaatg cgaattattt
     1441 agtacaaaaa gtgaacttct agtgagtagg ccaccggaac ctccccgaca gcctctcgca
     1501 gaaccgtacg tgaaactctc gcttcatacg gctcctgtca ggcaaacgcg cttcctggtc
     1561 ctgctttcca gtgggcaaat agctccggct tctctttcga cagtctttcc attaactgac
     1621 acgccttcgt ctttttatac cgcagggtct tgtatttctt tctcgcccac gcaatcagcg
     1681 tcagattaat atatctgcat actttcttca tcgctgattt gtggtatttc gcgtaataat
     1741 tcagccaccc cgatatcatc ggattcagcc ttttcgcaat atcttcaaga ctcatctcca
     1801 ccctgcatct gagccgcagt ttcctgagtt tttcgttcac gcctttctgc gcactatttc
     1861 tcaccgccgg gatgaaactc gtaaagaggt ttccccgcag gtggttacgt gctcttcgcg
     1921 ctctgaacgt tataccccag aaaatcaaac tgcgctgttt cgtggttcgc ctgacgttcg
     1981 ctgtttttgc agtaaactat tttcgttttc tccggatgta attccagtcc acattcctga
     2041 aaacgttttg ccagtttatt cagtaatttc tctgcctgtt gtttactgcc acaatgaatt
     2101 aatccatcat ctgcataacg acaccaggga atttccggat aatgcaacgt cagccattta
     2161 tcaaacacat agtgcagaaa taaattcgcc agtaccggac tgatgactcc gccctgtggc
     2221 gtacctttat ttctttccct cagtgttcca tccggcatct gcaccggcgc tgtcagccac
     2281 ctgcgaatat acagtcttac ccatttatcc gcgcaatgtt tatccactgc cctgagtaat
     2341 aaatcgtggg ggatattatc gaatagccct ttaatatcaa actcaagaca ccagtcgtat
     2401 tgccagcaac gttttctggt gactgctatt gcatccagcg ctgattttcc atgccggtat
     2461 ccgtatgaat cattcagaaa ataacgctcc actaatggtt caaacgttac cagcaccgtc
     2521 atctgcgcta ttcgatcgct tactgtcggt attcctaaaa gtcttttact gccatccttc
     2581 ttcggaattt ccaccgcacg cactggcggc ggcatccagc ttcccgacga taacctgttc
     2641 cagattttgt acagatttcc tttcagatcc cgctcaaaat ctttcagcgt ctggttatcc
     2701 actccggcag ctccggcatt cgcctttacc agccggtacg cttccaccac atgcaattta
     2761 ctgacctgaa acggttttgt ctgttcgccc gcagcctcct cctgtttaca gttgggctgt
     2821 gatgcagact gcccgacgct gccccttcgc tcgacgttca ttacaaacgc ttcctcacta
     2881 ctacgaacag ctctgccccg gtatcgcgca tcgatactct catactcata atttcggtta
     2941 tttgtatttc tctcttgaca ttgcgacgac cggttcccgt agttcctgca aagagcctga
     3001 tacagcgtca tgcctgctat acgccggaca ccatttacgc agcattcgga attcacccgt
     3061 aaattaatcc cggcggtatc acgaaacctc cggttttgat gtcacttagg actaacgacg
     3121 tttcaacaca ggttcgggtt tactcatctc actgtatctc acacaggatc agatcgcgtc
     3181 tgatcgttcc ccctgcgctc actaccatgg atctttacca cagcagccgg ggttgttagg
     3241 accgacttcc gacagcagcc tcgaatggcc aagcaccatc tctacacaag cttatgctta
     3301 ttacatgcta aacgtcccgt tttgcatctt tgggcttaca gcacagcgat tttttctatg
     3361 ataacaggta taatcatatt atcctcattc actcactaat taatttctaa ctctttaaaa
     3421 gttttaaaat atgcaacgaa tgaaaaaaaa taaaaccata atctaaaatt taagattgca
     3481 ccagtctgca aagtaagaca aataaaaaat agtataataa taatgaagtc gctaaaataa
     3541 aattgtttct ctttataaag ccaaatagcc aaggctaaaa ataaagttat agtgccgatg
     3601 atacccaaga atgaaattaa gtaaaatgtt gcattattcc caaccacaac attatcactt
     3661 ctaaagacat caagattacc taacccgatg ccaaaaagtg gatattcaga caatactctg
     3721 aacataaaat gcaaaggtgc aacaactctg attgttatac tattatttct gttggcatca
     3781 ttaaaatcta tatcagaaaa cctttctaag agaaaatcac taatcataaa caaaaatata
     3841 aaaacaaaca tgataaaaaa cacattaaat ataaatttgc ttttcttagt caaacttggg
     3901 aaatagatat ttttgttaaa aaaaacaata gaaaaataca ttaaaagtgt aagcgcagcg
     3961 cttggagtcc ggaagataat aaataaaatt aaattagtta taactaaaat ggctaatact
     4021 ataccactcc ttgaattaag gaggtagcaa caagttataa caaaaaaaaa taaataagct
     4081 aaggatgctt cttttgaaaa cacatttggt cttataaacc acttagtcat ttgtagatcc
     4141 cttacatgct ccatagcgat tatatctgat gttaccccaa aaaaaacttg acgataatag
     4201 tttgaaacat ttctagttac gtcaaataat tccaagacaa gaaaaataaa atatattatc
     4261 aatattcttt ttagaatatt tttatttcga cacaacttac ttatgagtaa caacctgtag
     4321 gtacaaacac caagaaatat tgagctggaa aattgtataa ttgcaccagt caaataacta
     4381 atttctccat aagcctccca tacaaatatt agcattataa aataataagt agttagaaac
     4441 ccaataaata tacaatctga ctgttttaaa tggataaatg gcacggacat tactaatata
     4501 cacatggcaa aaaccaaaat atccgaacct gcagaatatg atatatttat gtacacaaga
     4561 aatagtatca ttaaataaag agatgataac ctaattgaca tttaatcacc ctgtcagaga
     4621 catttttatt ctaatattct tggctatacc acgagcagca taaaatgaca gattaaaaaa
     4681 tacttttatt aatggcatct tatgtatata gcgtagtagc ataaattgct ttttcaactc
     4741 tttgatttta tttgaactta aagtatcacg ccctaccctg taaaaagcca tacagccagg
     4801 aaccttataa gctatatgtt cattttttaa aatctgaagc caaaaatcat aatcttcctt
     4861 aggtacattt ctaaaccgaa tattttcaat tatattcctt cgtataacta cggtaagaca
     4921 gccaattgga caatatttta gaagatcttt atacgacaat ttttcaggca cttcataagt
     4981 cgtatttaca aatcgattat gtcgaaatac tgcgtaatct ccgtatgtca tagaaacatt
     5041 tttcttaatc atgtaattaa tagtgttctc tagtttcgac tcgtgccata gatcatcact
     5101 atctagaaat gcaataaact caccttgtga atgacatatt cctgattgcc tagcacctgc
     5161 ggcaccttta tcaaaaaaat tattaatcaa aattactcgt gagtcctgaa tttgatatgc
     5221 ttggattata cgagtagtat catctgtgct attatcgtta attataatta attcgaagtc
     5281 taaatacgtt tgcctcagta cagactcaat agactctgcg attgtatatt gtgcattata
     5341 tgccggcatt ataatgctta tttttggctt atcatctaca tgcggcattc agtatatctc
     5401 ctgcatgcga atatgtataa ttatttctat atgctttaat agataaatat ggatcatatt
     5461 cagatagatt agagtaccaa gtattaaaat cagcgatcat atctttatac acatatatac
     5521 aaccactatc aatcacagct tcatatccaa tagcagaatg ccaactggaa taaatataca
     5581 ttccagcgga taaggcttct gcaatttttg ttttcatacc actaccatcc tctacaagag
     5641 tgatgtaccc cttacatgtt ttaaataact ccctcatata ctgttcactt ggagaacata
     5701 taaacttcac atttttaagg ttatttagat aaatatattt tgataatcct ctccctgcta
     5761 caatgaaatc aatagtgggg tagtactctg ctaatttgag aagtcgtttt aacgcaagca
     5821 catttggcga gaatgtaaag gaagctgtaa aaaggcaagc aggctttcta tttattattt
     5881 ctttctcata taaatcatca acacagacag gaataatact atacttaaca tcatcattta
     5941 gtttaaaagc attggcatca ttttttgtga taaaaaaaag atgactactt atttttttca
     6001 caaaacattc ggaaatttta atcaaaatgt aatcataata tcgaaatatc ttttcaaaaa
     6061 aaccatttgc taagacagcc tgttgcttgc tcaaattcga ctcattatta tctataaaga
     6121 aaaaaactct cacattaaat aataacttaa ggatcaaagc aacaattcct aatcgtgaat
     6181 tatggaaaaa taaatctaca cattcgcctt tatttttttt tatctccgct attatactgg
     6241 taatataaaa aaataaaaat gatggagata taaatattct tgatactatg tcagctattt
     6301 ttgttttttc taattgcaca ataaagccat tatattcttt tttgctttca ttggtcaatt
     6361 ctatcttttt atttataaca acaatttcag aatatttata ttttaacgca tttaatattg
     6421 ttcttgcata aatacctcca ccagtaagtt caaatataga gttgattgat ataaaataac
     6481 acttattttt attcattatg attttaccaa cactttaaga gctcgtattg cattccaggt
     6541 ttttacaaaa tctctaaccc acaatctctt caaaatataa aaaatgtgca ccatcagatt
     6601 tgtaaacaaa tattgcggat aatatctttt gataaaaagc aatcgactct gcaataaata
     6661 cttatctgaa atacttgaaa ctgcgtcaaa tgatttgctt gaagacccta tgctaccacc
     6721 ttctttatga taaatagttg ttgaattagt tattgtgaat tttatctttt tcttggctgc
     6781 tctagtaatt aaatcaagct cttcataata taaaaaatag gcctcattta acccacctaa
     6841 ctcaatgaaa cattcgcttt caatcaacag acatgcacct attggataat cgaccctaac
     6901 cgactcctta ataaagtcta gatcactaac gttctgctgt ctcccaacat gcttacaaac
     6961 accccacagt ttattaaaac taccgccaat agcttgaatt ttttcaggat catccaaata
     7021 aactaattga acacctatga ttttcacttg cttatctttt ttttgaacac ttatatattc
     7081 atttctcaat ttttcaattg agtcagttga gaacaggaca tctggattag caagcaagat
     7141 taatttactc tcgccatatt tacaaatgta atttgcacca agattatttc caaaagcata
     7201 gcctccatta actggggatt taatgaaata aatatctatt ttctcaaaca cgaataaaga
     7261 aactttgcaa tcaacattag aatgcttgat tgaaccatat tcttcattaa ttatgactgc
     7321 attattttta acccactccc ataaaacatt tatagagcta tctgtacttt tattatcaac
     7381 aactactatt tccctgggct ttaacgtttg ctgagataaa cttacaagac aattaatagt
     7441 atccgtaata ctattataat ttaatataac tgcacttaca tctaaatcat tcatctagta
     7501 gattacccca cttttctgat agtttatcaa ttgtaaaatc ctttgctcga ttataggagg
     7561 caagcgacat ttttttccat gtttcataat ctaatgtatc aataacttca atagcacatg
     7621 aaagatatgt ctcttctgta aatataaaac cttgcttatt atctatgatt tcaagaggcc
     7681 ctggttgatt tgttgcaatc acaggaatac ctcgggacat agcttcaata acagttaatc
     7741 caaaagcttc tttccagcct ttgcaactaa ttgatggctg caaaagaaat gaacatctat
     7801 caacaatttc ttttattttt tccagttcgc ttagatgccc gtgaataatt atatttttct
     7861 gatttttaag gacatatgaa tctggtccat caccaataat atgaaatgta tatcttgggc
     7921 aagccttagc gatttttaaa atatttacta agcctttttc tttaactaag cgccccataa
     7981 aaagaatatg acgttttttt tctttgagta ttatttcagc ctcatccatt agccatcctt
     8041 gttttaagga atggtataca acttctgaat tatatttatc aaaacaaaag taatttataa
     8101 attctctata tgtttttctg ctaaccgcaa agaaacctgc tatattattt ttcagagcat
     8161 aagtccatac tttttttgca acacatgagt tacctttagg aaactgcccg tcttgccact
     8221 caggccaaga gttatgaata taaactttat tattcctaaa tatccataat agcaatgcca
     8281 atcttaaatc aagaggcgca actccaagta taattatttc atttcgctta tttgatatca
     8341 aacgccttaa taaaaaacac aagttactaa aaaatcgcct cagatttttt aatctatttt
     8401 tttttattat cgctttgatt tgaggtgtaa taccaaattc gtgaaatttt aactcataat
     8461 tattattatc acagaaatat tttaatgcag tatagtggtt tagagctcca tattcgtgaa
     8521 ccacaattat tcttaatgtt tccacaatat attttctcgc actatcttat ttaattcacc
     8581 agatataaca gtacgttctt taacaatttt cttgtgtaat ttagtaccag cggaaattac
     8641 acacccattt gcaatattga cacctttcat tattaaacag cgacacccaa tccatacatc
     8701 atcaccaata ttaattggct cttttactcc tgactcgcta tactctccat tatttagcac
     8761 agagtaaacc tgatgaaaat cagtatccat gatcaataca tcccaagaaa ttaagcaatt
     8821 tataccaaat tctatcttac tagagcaaat caaagatgat tctccagata taataaaatc
     8881 ttttccaaat attaactttg ccccgttgtt tatatttatt tttgtaccat gtccaatatt
     8941 tgtttttcct cgaaagataa gagttccgtc attttttatg atcgtttttg aattataccc
     9001 atcaaatata ccaatatcac caaacccaat ttttattaga ccaaaagcag gttctgtatc
     9061 aagaataatt tttccaattc caactagttt cacattcttt gcaactaaca ctgggaattt
     9121 aattgctgtc caaaatgaaa aacagctaaa atttaaatat aatgttttat gaaaccctaa
     9181 tataaatgat aatatctttt cctttgtact caatttatta accctcatta gtaattttta
     9241 caggagaaat aaaaaataat ctaattgttg aaaaaacatt tacagttata accgaaagcc
     9301 ctaaagaaat gcatgcaatg acattggaat taccaaataa gaacgggctt gttaatgcaa
     9361 ttaaaatact gatcgataga gctgccacat atattttgag aagaatattt tcattatcaa
     9421 tatataatag tatgctacca ttcacagcac agtatgcatt tatcaattga aataatacaa
     9481 gaatttttaa attcacactg gctccatcaa aagattcgcc atatatagat attatataat
     9541 ccgctgtaat atatacaaca atcgccatca acgcgttagt tattattgct atttttttac
     9601 atttccttgc tattttgaag aactcttttt tattattttt aaatgaatta atcattttag
     9661 gaataaacaa cattacaatg gtggttgtta caaagttaaa gagtaatgca attttctgcg
     9721 caacaataac ttttgatagt tctgaataag ataaaaaata tccattgatt attacaacgc
     9781 cctgagataa tgctattata attatattga tcatggcata tttaaaagtg ccatgtttaa
     9841 atttaattga acactttaaa aattgaaaat ctccttcagc ttttgctgaa catatagcat
     9901 aaataaaaac caaaatacaa cttaaaagat aaatagaact aattatgata attttgttat
     9961 aatcaatggt atttaaaaaa tgtaacaacc atatacaaaa aattaatatg gctggaacag
    10021 caaacgactg aatgattacc attggtgtaa atctttcttt tgcttgtaaa taactagcaa
    10081 atgaattaga acatgccata aaaaatatag caggtgacat tagcaaaaga gtccaatata
    10141 aatctttctc cccgttaaca ttcgcaatca gaaagattaa tgcaaaaaaa agaaatgcaa
    10201 ttgctaaagc gcaatataat gatgagctaa tcaggatact ttctcgtgat ttatcaatat
    10261 attgatcact aacctgtttt aatatcatag tattaaagcc aaaattagct accacagata
    10321 aaacacttat aatattcaaa gaaacataat atattccact atcatgagcc ccaagatacc
    10381 gcgacactaa aattgtggat ataaaagata aggcagcacc tccacctcgt aaaaaaaaag
    10441 gaattacttt tgaatgaaaa taatttttta ctctagatgc tttgttttgc attttgtata
    10501 aattcaactc tcagtttatt taaattagca tctttttccg ataaaaccgg attctcacac
    10561 atgggccatt ttatatttat aagttcatcc tcccatgata taccacattc ataatcttta
    10621 ttataaaaag ctgtcgtctt atacaaaaac tccgccgttt cactcagcac caaaaaccca
    10681 tgcgcaaatc cctctgggat ccacaactgg cgcttattct cagcagataa attcacacca
    10741 acccacttgc caaacgtagt tgacgactta cgaatatcaa ccgcaacatc aaaaacttca
    10801 ccgacaacac agcgtaccag ttttccttgt gcataaggtt ctaactgata atgcagacca
    10861 cgtaaaaccc ctttggaaga tttagaatgg ttatcctgaa caaactcaac tttgcgacct
    10921 accgcttcct caaaaatctt ttggttaaaa ctttcaaaaa agaatccacg ttcatcacca
    10981 aatactttcg gttcaaaaat cagtacgtct ggaatttctg ttttaataac gttcatttta
    11041 ttactaacct ttaatcattt tcagcaaata ctgaccataa gcatttttct tcagcggttc
    11101 ggctaatacc ttaacctgct ctgcatcaat aaacccttta cggtaagcaa tttcttctgg
    11161 gcaggaaacc tttagtccct ggcgctcttc aatggtggca atgaagttgc ttgcttcaat
    11221 aagactctga tgtgtccccg tatccagcca tgcataacca cgccccatca tggcaacgga
    11281 taaacgcccc tgttccatat aaatacggtt aatatcggta atttccagtt caccacgggc
    11341 agaaggctta aggtttttcg ccatttcgac aacgtcgtta tcatagaaat aaagcccggt
    11401 taccgcataa ttactttttg gttgtagcgg tttttcttcc aagctgattg ccgtaccgtt
    11461 tttatcaaac tcaacaacac cgtagcgttc aggatcatta acgtgatagg caaataccgt
    11521 tgcaccactt tctttgttga cagcgacatc cattaacttc ggcaaatcat gaccgtagaa
    11581 gatattatca ccaagaacca aagcacaatc atcaccaccg ataaactctt caccgatgat
    11641 aaatgcctgc gcaagaccat ctggactcgg ttgcactttg tactgaagat ttagccccca
    11701 ctggctacca tcacccagca gttgttgaaa acgaggagta tcctgtgacg tactgataat
    11761 cagaatatcg cgaatacccg ctaacatcag tgtagaaagc gggtaataga tcatcggttt
    11821 atcataaatc ggtaacagct gtttgcttac agccatagtc acaggataaa gacgagtacc
    11881 agcccccccc gctaaaataa tacctttacg cgttttcatt tcatcattcc ttttaattca
    11941 tcttgttcca ccatcacgaa caagatgcaa aaactattaa attgctgtag tcataaataa
    12001 ttcgttaagc attcgtttca cgccaacctg ccagtcaggc aagacaagcg caaagttctg
    12061 ctgaaatttt tctgtattaa gacgagagtt atgggggcga cgggctggtg taggataggc
    12121 tgttgttggt actgcattga gcttgttgag tgcaagggga atgcctgctt tgcgcgcctc
    12181 ttcaaaaacc agcgcagcat aatcgtgcca agttgtggta ccactggcta ccagatgata
    12241 caaacctgcg acttctggtt tattcagtgc cacacgaatt gcatgtgccg tacaatcagc
    12301 cagcaactct gcgcccgttg gcgcaccaaa ctgatcatta ataacggcta gttcttcgcg
    12361 ctcttttgcc agacgcaaca tcgttttggc gaagttattt cctttacctg catagaccca
    12421 gctggtccgg aaaataaggt gcttcgcaca atgctcttgt aatgcttttt ctccggctaa
    12481 cttggtttct ccataaacgt tcagcggtgc ggttgcatcc gcctcctgcc atggtatttc
    12541 acctgttccc ggaaatacgt agtcagtaga gtagtgaata gcccaggcgc cgacttcatt
    12601 ggctgctttc gcgatcgctt cgacactcgt cgcgttaatt aattgtgcaa actccggttc
    12661 tgattctgct ttgtctactg cggtgtgagc ggctgcgttg acaataatat caggccgaat
    12721 gcttcttacg gtttcagcta caccctcagg attactaaaa tcaccacagt aatcagtgga
    12781 gtgaacatca agagcaatca aattacccag aggtgccaga gcacgctgta gttcccaacc
    12841 tacctgccct gttttgccga aaaggaggat attcattact ggcggccctc ataattctgt
    12901 tcaatccacg attgataggc accacttttc acattatcaa cccattttgt attggacagg
    12961 taccattcca ccgttttatg aatcccgctc tcaaacgttt cctgcggttt ccagcccaat
    13021 tcgcggctaa tcttctctgc atcaatcgca taacgacgat cgtgtcccgg gcgatcggca
    13081 acataggtaa tttgctcgcg gtaagatttc tctttcggta caatctcatc caacaaatca
    13141 caaatagtga gcactacatc gatgtttttc ttttcgttgt gtccaccaat gttataagtt
    13201 tcacccgctt taccttcggt tacgacggta tataacgcac gcgcatgatc ttcaacatac
    13261 agccagtcac gaatttgatc ccctttgcca taaataggta atgccttacc ttccagagca
    13321 ttaagaatta ctagtggaat caatttttcc ggaaagtgat aaggaccgta gttattcgaa
    13381 cagttagtca caatggtcgg taaaccatag gtacgtttcc acgcacggac taaatgatcg
    13441 ctggatgctt ttgatgcgga ataaggacta ctaggcgcgt aagctgtcgt ctcagtaaag
    13501 aggggtaatt gttctttatt atttacttcg tcaggatgag gcaaatcacc atagacttcg
    13561 tcagtagaaa tatgatgaaa acggaagcta tttttcttgt cgccatcaag agcagaccag
    13621 taattgcgag ccgcttccaa aaggacataa gtaccaacaa tattggtttc aataaatgcc
    13681 gcagggcctg taattgaacg atccacatgg ctttcagcag ccaagtgcat cactgcatcc
    13741 ggctgatgct gagcaaaaat ccgcgccatt gcagcagcat cgcaaatatc cgcatgttca
    13801 aaaacatagc gttcagagtc agaaacatca gcaagtgatt ccaggtttcc ggcgtacgtt
    13861 aatttatcga cattaacaac actatcctga gtattattta taatgtgacg aactacagca
    13921 gaaccaataa atcctgcgcc accagtaaca agtattttca cttaatttat tccatattac
    13981 ttcagagcat gctgtgaaat aagcggctct cagtttgatt aatagaggta ttaatgcacg
    14041 ctaccgcccc tggctttaca gctaccag
//
