LOCUS       KJ739599               13948 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain PF11-6E serotype OX38:H47 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ739599
VERSION     KJ739599.3
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13948)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Swimley,M., Burrell,A.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
REFERENCE   3  (bases 1 to 13948)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Swimley,M., Burrell,A.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
  REMARK    Sequence update by submitter
REFERENCE   4  (bases 1 to 13948)
  AUTHORS   Yan,X., Baranzoni,G.M., Fratamico,P.M., Debroy,C., Tebbs,R.S.,
            Liu,Y., O'Connell,C.D., Swimley,M. and Matheny,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2015) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
  REMARK    Sequence update by submitter
COMMENT     On Jun 18, 2015 this sequence version replaced KJ739599.2.
            
            ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: 50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PF11-6E"
                     /serotype="OX38:H47"
                     /db_xref="taxon:562"
     misc_feature    1..13948
                     /note="O-antigen gene cluster"
     gene            118..1239
                     /gene="wbnE"
     CDS             118..1239
                     /gene="wbnE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AIG56930.1"
                     /translation="MIKILHIHLSSKISGAQRVSLDEMKTLSNHYSQYMVCSKEGDFT
                     QEADRIGVKTYVIETLVREISPLKDFYSLIKLYKFIKQEKFDIIHTHSSKSGFLGRLA
                     AKLAGTKQIIHTVHGFAFPSTSNRVVKLIYFLMEYFASLCSSVIIVMNENDEKIARKY
                     FSSAPWTKVTLLNNAVDIKKFQKRYIGIESKSEINEQKKFKMVMIGRLCEQKNPLLII
                     NALKILGDHYYVDFVGDGPLRSDLESQIAKRGLEKRVRLLGWCSSVEEIIFKYDLFLL
                     PSKWEGMPLAILEAMASKVPVLCSNIDANAYLINKTSGFLFNNDDAKDLAKNIKYIFD
                     NVDVRRKVAEDAYLAVVKDFELSERTKILESIYTNNYFL"
     gene            1252..2376
                     /gene="gmd"
     CDS             1252..2376
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="AIG56931.1"
                     /translation="MDKNKIALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTE
                     RVDHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPE
                     YTADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYA
                     VAKLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCL
                     YLGNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAEQVGIKL
                     RFEGVGVNEKGIVVSVTGKDAPAVKSGDIIVKVDPRYFRPAEVETLLGDPTKAHEKLG
                     WKPEITLREMVSEMVANDLEAAKKHSLLKSHGFNVSLSQE"
     gene            2380..3345
                     /gene="fcl"
     CDS             2380..3345
                     /gene="fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="AIG56932.1"
                     /translation="MFLQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            3348..3848
                     /gene="nudD"
     CDS             3348..3848
                     /gene="nudD"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="AIG56933.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASENVHANSRVYFNNDPRAIIGLNK
                     KEVKNV"
     gene            3841..5289
                     /gene="manC"
     CDS             3841..5289
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AIG56934.1"
                     /translation="MSEVPLIAVVMAGGTGSRLWPLSREHYPKQFLQLSGESSLLQST
                     LLRLSTLSCETPLVITNEQHRFVVAEQLRQIDQLRDNIILEPCGRNTAPAIALSAFTA
                     LKRNEQQDPILLILAADHIISKADVFCDAIQKSKTVAEQGNIVTFGIIPEYAETGYGY
                     IKKGDPFKILPNITHDKFYHVQQFIEKPNHSTAEEYVSTGEYLWNSGMFMFKASVYLE
                     ELKKHRPDIYEICEQTASLSYNDLDFIRLPKHIFQDCPSESIDFAVMEKTKNCIVRPV
                     DIGWSDVGSWHSLWDISDKTQDGDVCKGDILTYNTKNNYIYSESALVAAVGVEDIVIV
                     QTKDAILVSKKTEVQDVKKIVEMLKEQERSEYISHREEFRPWGKFDAIDQGERYKVKK
                     IVVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLNDKTVLLAANESIYIPLGATYSLENP
                     GIIPLNLIEVSSGDYLGEDDIVRQKERYRIDD"
     gene            5293..6672
                     /gene="algC"
     CDS             5293..6672
                     /gene="algC"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="AIG56935.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQITLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDVIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLADPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGNIALMNEQVEFI
                     TKFLNGDGL"
     CDS             6669..7226
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="putative O-acetyltransferase"
                     /protein_id="AIG56936.1"
                     /translation="MRILKIIISVLLFIKKKRKKIPDSAIINLTSHVDKHSVIGKCCY
                     IGKNVYITKSIIGNYTSIANNVSIGQGEHKVDAVSTSSMFYDNEFEDLTQNDCIIGND
                     VWLGTGVTVLRGVRIGDGAIIGANAVVTKNIPDYCIAVGVPAKVIKRRLPVNVTEKIK
                     ETKWWNDEYEVAKDKIICLTNELKL"
     gene            7223..8665
                     /gene="wzx"
     CDS             7223..8665
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AIG56937.1"
                     /translation="MNKKEIIILLIARILQILISLFTMRLMTTILSNEQMGEYYIFLA
                     IYMLASLGIINPIGQYVNRNTYLWWREGVLTQYINRYINFVLCFSLIYVILYGLGECL
                     FKGINTPFWMLFCYLYIVCLSLNQFLLHTLNILNFKIVFAIMTLMTSVLSLSGAYLFI
                     EYSIAKQTVSIYYWVAGIIIGNFISLCICVVYLRSKNTFQKSILKSINVSIKEVFNFC
                     CPIMITTLFIWFQSSGYRFAVEQMFGLKFLGILAVCFAVSGQIMSVIESLVTQIFQPT
                     LFKHMDNVKNRKQHLDFYINENIAIYASVLFFCTFFMREIFLVLVDNKYLPYFYIGII
                     ALWSEFFRISTNTLSLVFFCEKKMKVGIFPYLIGSLSLAFSIVLFRLLNVNLGILIAF
                     AIGVAQLASFIVCLITVKKISYFKINSIFLMKRIIIVLPSVLIAHCIPKFTFNNAIHF
                     IIIFGVGLFYLISLVTSFMFIKQPGGVYGS"
     CDS             8655..9506
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="glycosyltransferase"
                     /protein_id="AIG56938.1"
                     /translation="MEVKIIGGLGNQMFQYATAFAIAKRTHQNLTVDISDAVKYKTHP
                     LRLVELSCSSEFVKKAWPFEKYLFSEKIPHFMKKGMFRKHYVEKSLEYDPDIDTKSIN
                     KKIVGYFQTEKYFKEFRHELIKEFQPKTKFNSYQNELLNLIKENDTCSLHIRRGDYVS
                     SKIANETHGTCSEKYFERAIDYLMNKGVINNKTLLFIFSDDIKWCRENIFFNNQICFV
                     QGDAYHVELDMLLMSKCKNNIISNSSFSWWAAWLNENKNKTVIAPSKWFKKDIKHDII
                     PESWVKL"
     gene            9503..10369
                     /gene="epsE"
     CDS             9503..10369
                     /gene="epsE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AIG56939.1"
                     /translation="MMEDTKELITVIMPVYNAEKYIYESIKSILVQSYSNFEFIIIND
                     GSKDSSGKIIENFLDDTRIRYINRENKGLVFTLNEAISLSRGNYIARMDADDISHPLR
                     LEKQLNFLLENPDIAVVGCSSFIIDQNSKVINTRKPPLTPLVNKALLFFGPTLTHPSV
                     MFNKMLLGDQLYYSDKYLHVEDYDLWLRLINQYKIANIKDVLFYYRINESGVSQTNLF
                     EQKINAAKAYSEIKYDGKYKDLLEKLEVIHLRHEMEKSKVFQAVLYILLKYEHDSLLF
                     LKLGYILRWLIK"
     CDS             10354..11253
                     /codon_start=1
                     /transl_table=11
                     /gene="orf11"
                     /product="glycosyltransferase"
                     /protein_id="AIG56940.1"
                     /translation="MVNKIKIALISTEKANLPEIEAYKEYFSEPFDVSVVNKNDDLSC
                     YDVLWYFMGFKGNKINSKQFLIHEFSSISVPPFPKIKNVIKKFILPKPNLRVFLNKSV
                     QQQFDFNDRVPFCYRDMGVNYKPVVERNNKKYDILYVGAITGRKLDDALNLILSYKLD
                     CKIAIFGKVPEKFKEKYKNADVIFLGQVPYNRVATIIAESNLCLNWIPNIYPYNMQTS
                     TKFLEYLAQNKQIISNSYPWVDSYCQQHNIDYININDSNSIRQALNSVIIQKSTHIPP
                     SWREVIMNSGIENHIKSYFEVTK"
     gene            11250..12293
                     /gene="wzy"
     CDS             11250..12293
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AIG56941.1"
                     /translation="MISYGVLFLFSYINAVLQKRVTVTLLFFIMIIVSFFKYNIGFDW
                     GNYHEIYLQQGDGFFFYEPLFSLLIILSNKVALEYKYFISMCSLITLIVYYSGMRCFA
                     RDIKELALALTYFVVSYCYFEHFNIIRQTLAIALFFYSWKYIIETNFIMYSLFIFFAS
                     MIHAAAVVLLPFYFFLRIDNFKPVYYGIVVLFIIIVVRFDINSILLSSLNLFKNFDFV
                     ARYLANSTDLADFASKKSDLGLSKMYPAIIFVFCCFWGGIRLESRRDYVFFNAGFIAI
                     TLLLLSYQVDILFRFFSFFETSFVLLYCIIFSGFNKKSKIILHMVNLIVYTVMTFNML
                     SVMGLIPYESYIF"
     gene            12763..13782
                     /gene="galE"
     CDS             12763..13782
                     /gene="galE"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AIG56942.1"
                     /translation="MAILVTGGAGYIASHTILSLIEQGNEVIIIDNLSNSYYDSLLKI
                     KEITGCDCLFYKGDILDKKLLLKIFDENNITAVMHFAGAKSVSESVIRPLQYYRNNVS
                     GTFSLVEAMEESGVENLIFSSSATVYGEPKIIPVNESCAIGGTTNPYGTSKLFVENFL
                     RDYSKASPNFKTIVLRYFNPIGAHSSGKIGEDPNGIPNNLMPFICQVAIGKQKTLKIY
                     GNDYPTKDGTGIRDYIHVMDLAEGHVAALNNINQGANYRVYNLGTGIGYSVLELLEAF
                     QKVTTRRVPYVFTKRRSGDIAECWSDPTKAYEELGWKARRGLEDMIRDAWNWQQKNPN
                     GFKKV"
BASE COUNT     4352 a   2344 c   2874 g   4378 t
ORIGIN
        1 ctggtagctg taaagccagg ggcggtagcg tgtttatttc atattggtgc agctagttta
       61 ccattatata attgtaagtt aatcacttcg aatgtgcatt ttcaggaatt agatctaatg
      121 attaaaatat tgcatataca tcttagtagt aaaattagcg gagcccaaag agtttcttta
      181 gatgaaatga aaacgttgag taatcattac tcacagtata tggtttgctc caaagaaggc
      241 gattttactc aagaagcgga tcgtattgga gtaaaaactt atgtaataga aaccttagtc
      301 agagaaattt cacctctaaa agacttttat tcattaataa aattatataa atttatcaag
      361 caagaaaaat tcgatattat tcatacgcat tcctcaaaaa gtggttttct cgggcgatta
      421 gcagcaaaac tcgcgggcac aaaacaaata atccatacgg tgcatggatt tgcgtttcct
      481 tcaacatcaa atagagttgt aaagcttata tattttttaa tggaatactt tgcttcatta
      541 tgttcatcag tgattatcgt aatgaatgag aatgatgaga aaatagccag aaaatatttt
      601 tcttctgctc cttggaccaa agtgactctc ttaaacaatg ctgttgatat aaagaagttt
      661 caaaagcgat atattggaat cgaatcaaaa agtgaaataa atgagcaaaa gaaatttaag
      721 atggtcatga ttggacgctt atgtgagcaa aaaaacccac tattaattat aaatgcgtta
      781 aaaatattgg gtgaccatta ttatgtggat tttgttggtg atggcccact aagatcagac
      841 ttagaaagtc aaatagcaaa acgtggtctt gaaaaaagag tccgtttatt aggatggtgc
      901 tcgtctgtag aagaaattat ttttaaatat gacttgtttt tattgccttc aaaatgggaa
      961 ggaatgccat tagctatact tgaggcaatg gcatcaaaag taccggtttt gtgttcgaat
     1021 attgatgcta atgcatatct tattaacaaa acttcaggtt ttctttttaa caatgatgat
     1081 gctaaagatc ttgctaagaa tataaaatat atttttgata atgtagatgt acgtagaaaa
     1141 gttgcagagg atgcatattt ggcagtagtt aaggattttg agttatcaga acgtacaaag
     1201 atattagaat caatttacac taataattat tttttataag tgaggttatt tatggacaaa
     1261 aataaaattg cactcatcac cggtgtaacc ggacaagatg gttcttacct ggcagagttt
     1321 ctgctggaaa aaggttacga ggtgcatggt attaagcgtc gcgcatcgtc attcaacacc
     1381 gagcgcgtgg atcacattta tcaggatccg cacacctgca acccgaaatt ccatctgcat
     1441 tatggcgacc tgagtgatac ctctaacctg acgcgcattt tgcgtgaagt acagccggat
     1501 gaagtgtaca acctgggcgc aatgagccac gttgcggtct cttttgagtc accagaatat
     1561 accgctgacg tcgacgcgat gggtacgctg cgcctgctgg aggcgatccg cttcctcggt
     1621 ctggaaaaga aaacccgttt ctatcaggct tccacctctg aactgtatgg tctggtgcag
     1681 gaaattccgc agaaagaaac cacgccgttc tacccgcgat ctccgtatgc ggtcgccaaa
     1741 ctgtacgcct actggatcac cgttaactac cgcgaatcct acggcatgta cgcttgtaac
     1801 ggtattctct tcaaccatga atccccgcgc cgcggtgaaa ctttcgttac ccgcaaaatc
     1861 actcgcgcaa tcgccaacat cgcccagggg ctggagtcgt gcctgtacct cggcaatatg
     1921 gattccctgc gtgactgggg ccatgccaaa gactacgtaa aaatgcagtg gatgatgctg
     1981 caacaggaac agccggaaga tttcgttatt gccaccggcg ttcagtactc cgtgcgtcag
     2041 ttcgtggaaa tggcagcaga gcaagtcgga atcaaattgc gctttgaagg tgtcggcgta
     2101 aatgagaagg gaatcgttgt ttctgttact ggtaaggatg ccccagccgt taaatccgga
     2161 gatataattg ttaaggttga cccgcgctat ttccgtccgg cagaagtaga aacgctgctc
     2221 ggcgacccga ccaaagcgca cgaaaaactg ggctggaaac cggaaatcac cctcagagag
     2281 atggtgtctg aaatggtggc taatgacctc gaagcggcga aaaaacactc tctgctgaaa
     2341 tctcatggat ttaatgttag tttatctcag gaataactaa tgtttttaca aagaattttt
     2401 attgctggcc atcgcgggat ggtcggttcc gccatcaggc ggcagctcga acagcgcggt
     2461 gatgtggaac tggtattacg cacccgcgac gaactgaacc tgctggacag ccgcgccgtg
     2521 catgatttct ttgccagcga acgtattgac caggtctatc tggcggcggc gaaagtgggc
     2581 ggcattgttg ccaacaacac gtatccggcg gatttcatct accagaacat gatgattgag
     2641 agcaacatca ttcacgccgc gcatcagaac gacgtgaaca aactgctgtt tctcggatcg
     2701 tcctgtatct acccgaaact ggcaaaacag ccgatggcag aaagcgagtt attgcagggc
     2761 acgctggagc cgaccaacga gccttatgcc attgccaaaa tcgccgggat caaactgtgc
     2821 gaatcttaca accgccagta cggacgcgat taccgctcag tcatgccgac caacctgtat
     2881 ggcccgcatg acaacttcca cccgagtaat tcgcatgtga tccctgcatt gctgcgtcgc
     2941 ttccacgagg cgacggcaca gaatgcaccg gacgtggtgg tatggggcag cggtacaccg
     3001 atgcgtgaat tcctgcacgt cgatgatatg gcggcggcga gcattcacgt gatggagctg
     3061 gcgcatgaag tctggctgga gaacacccag ccgatgctgt cgcacattaa cgttggtacg
     3121 ggcgttgact gcactatccg cgagctggcg caaaccatcg ccaaagtggt gggttacaaa
     3181 ggtcgggtgg tttttgatgc cagtaaaccg gatggcacgc cgcgcaaact gctggatgtg
     3241 acgcgcctgc atcagcttgg ctggtatcac gaaatctcac tggaagcggg gcttgccagc
     3301 acttaccagt ggttccttga gaatcaagac cgctttcggg ggtaatgatg tttttacgtc
     3361 aggaagactt tgccacggta gtgcgctcca caccacttgt ctctctcgac tttattgtcg
     3421 agaacagtcg cggcgagttt ctgcttggca aaagaaccaa ccgcccggcg cagggttact
     3481 ggtttgtgcc gggagggcgc gtgcagaaag acgaaacgct ggaggccgca tttgagcggc
     3541 tgacgatggc ggaactgggg ctgcgtctgc cgataacagc aggccagttt tacggtgtct
     3601 ggcagcactt ttatgacgat aacttttccg gcacggattt caccactcac tatgtggtgc
     3661 tcggttttcg cttcagagta gcggaagaag agctgttact gccggatgag cagcatgacg
     3721 attaccgctg gctgacgccg gacgcactgc tcgccagtga aaatgttcac gctaacagcc
     3781 gtgtttattt taataatgat ccacgtgcaa tcattggctt aaataaaaag gaagtcaaaa
     3841 atgtctgaag tacctttgat tgctgtcgta atggctggtg gtacggggag ccgcctttgg
     3901 cctctgtcta gagaacacta tcctaagcaa tttttgcaac tatcaggtga gagttcttta
     3961 ttgcaatcaa ctttattacg actgtcaact ctttcctgcg aaactccatt agtaataaca
     4021 aatgagcaac atcgctttgt ggttgcagaa caattacgtc aaattgatca gttaagagat
     4081 aatattattc ttgagccatg tggaagaaat acggctcccg ccatagctct ttcagcattt
     4141 acagctttga agcgtaatga acaacaggat cctattttgt tgattctcgc agctgaccat
     4201 attataagca aagcggatgt tttttgtgac gctattcaaa aatcgaaaac tgtagctgaa
     4261 caggggaata ttgtaacctt tgggattata cctgaatatg cggaaacagg ttatggttat
     4321 ataaaaaaag gagatccttt taaaatttta ccaaatatta ctcatgataa gttttatcat
     4381 gtccaacaat ttattgaaaa acctaaccat tcgacggcag aagagtatgt ttccacagga
     4441 gaatatctct ggaatagtgg aatgtttatg tttaaagcat cagtatattt agaggaattg
     4501 aaaaagcaca ggcctgatat atacgaaatc tgtgagcaga cagcttcatt atcctataac
     4561 gatcttgatt tcatacgatt gcctaaacat attttccaag attgtccgtc ggaatcaatt
     4621 gattttgcgg ttatggaaaa aacaaaaaat tgcattgttc gtccagtaga catcgggtgg
     4681 agtgatgtag gctcttggca ttctttgtgg gatattagtg ataaaacgca ggatggagat
     4741 gtctgtaaag gtgatattct aacttataac actaaaaaca attatatata ttctgaatca
     4801 gccctagtgg ctgctgtagg tgtagaagat atagttattg ttcaaacaaa agatgctatt
     4861 ctcgtgtcca aaaaaactga ggttcaggat gtaaaaaaaa tagtagaaat gcttaaggaa
     4921 caagagcgct cagagtatat atcgcatcga gaggagtttc ggccttgggg aaaatttgat
     4981 gcgatagatc agggagaacg atataaagtt aaaaagatag ttgtgaaacc cggcgaagga
     5041 ctatccttac gaatgcacca tcatcgttct gagcattgga ttgttctttc cggcacagca
     5101 aaagtaactt taaacgataa aacagtattg cttgctgcaa atgaatcaat ctacattcct
     5161 ctaggagcaa cgtatagtct tgaaaatcca ggaataatcc ctctgaatct tattgaggtt
     5221 agttcaggcg attatttagg tgaggatgat attgtacggc aaaaagaacg ctacagaata
     5281 gatgactaat atatgaaaaa attaacctgc tttaaagcct atgatattcg cgggaaatta
     5341 ggcgaagaac tgaatgaaga tatcgcctgg cgcattggtc gcgcctatgg cgaatttctc
     5401 aaaccgaaaa ccattgtatt aggcggtgat gtccgcctca ccagcgaaac cttaaaactg
     5461 gcgctggcaa aaggtttaca ggatgcgggc gtcgatgtgc tggatattgg catgtccggc
     5521 accgaagaga tctatttcgc cacgttccat ctcggcgtgg atggcggcat tgaagttacc
     5581 gccagccata acccgatgga ttataacggc atgaaactgg tgcgcgaagg ggctcgcccg
     5641 atcagcggtg ataccggact gcgcgacgtc cagcgtctgg cagaagccaa cgacttcccc
     5701 cccgtcgatg aaaccaaacg cggtcgctat cagcaaatta ccctgcgtga cgcttacgtt
     5761 gatcacctgt tcggttatat caatgtcaaa aacctcacgc cgctcaagct ggtgatcaac
     5821 tccgggaacg gcgcagcggg tccggtggtg gacgttatcg aagcccgctt taaagccctc
     5881 ggcgcaccgg tggaattgat caaagtgcac aacacgccgg acggtaattt ccccaacggt
     5941 attcctaacc cgctactgcc agaatgccgc gacgacaccc gcaatgcggt catcaaacac
     6001 ggcgcggata tgggcattgc ctttgacggt gattttgatc gctgcttcct gtttgacgaa
     6061 aaagggcagt ttattgaggg ctactacatt gtcggcctgc tggcagaagc attcctcgaa
     6121 aaaaatcccg gcgcgaagat catccacgat ccacgtctct cctggaacac cgttgatgtg
     6181 gtgaccgccg cgggcggcac tccggtgatg tcgaaaaccg gacacgcctt tattaaagaa
     6241 cgtatgcgca aggaagacgc catctacggt ggcgaaatga gcgcccacca ttacttccgt
     6301 gatttcgctt actgcgacag cggcatgatc ccatggctgc tggtcgccga actggtatgc
     6361 ctgaaaggaa aaacgctggg cgaactggtg cgcgaccgga tggcagcatt tccggcaagc
     6421 ggtgagatca acagcaaact ggcggacccc gttgaggcga ttaaccgcgt ggaacagcat
     6481 tttagccgtg aggcgctggc ggtggatcgc accgatggca tcagtatgac ctttgccgac
     6541 tggcgcttta acctgcgctc ctccaacacc gaaccggtgg tgcgcctgaa tgtggaatcg
     6601 cgcggtaata tagcattaat gaatgaacag gttgaattta ttacaaaatt tttaaacggt
     6661 gatggtttat gagaatatta aaaattatta taagtgtatt gttgtttata aaaaagaaaa
     6721 gaaaaaaaat accggactca gcaataataa acttaacaag tcatgtggat aaacattcag
     6781 taattggaaa gtgttgttat atagggaaaa atgtatatat aactaaaagt atcattggga
     6841 attacacatc tattgcaaat aatgtctcaa ttggtcaagg tgaacataag gttgatgcag
     6901 ttagtacaag ttcgatgttc tacgataatg agttcgaaga tttaacacaa aacgattgta
     6961 ttataggtaa tgatgtgtgg ctcggtacag gagttactgt attacgagga gtaagaattg
     7021 gtgatggtgc tattattggt gctaatgccg ttgtcacaaa aaatattcca gattattgta
     7081 ttgcggtagg agtacctgcg aaagtaatca aaagaagatt accagtaaat gttactgaaa
     7141 aaataaaaga gactaaatgg tggaatgatg aatatgaagt ggcgaaggat aaaattattt
     7201 gcttaacaaa tgaattaaag ttatgaacaa aaaagaaata attatcttgc ttatagccag
     7261 aattctacag attctcatta gtttattcac aatgagattg atgacaacga ttttaagcaa
     7321 tgagcaaatg ggggagtatt atattttttt agcaatctat atgttggcat cattgggtat
     7381 aattaatcca ataggacaat atgtgaatcg caacacttat ttatggtgga gagagggagt
     7441 actaacccaa tacattaatc gatatattaa ttttgttttg tgtttttcgc ttatttatgt
     7501 gattttgtat ggacttggtg aatgtttgtt taaaggaata aatacaccat tttggatgtt
     7561 attctgttat ttatatatag tatgtttatc tctaaatcaa tttctattac atactttaaa
     7621 tatcttaaat tttaagatag tatttgccat tatgacgttg atgacttctg tattaagttt
     7681 atcaggggca tatttattta ttgaatatag catagctaaa caaactgtta gtatatatta
     7741 ctgggttgca ggaatcataa ttggcaattt tatttcttta tgtatatgcg ttgtatatct
     7801 gagaagtaaa aatacgttcc aaaaatcaat tcttaaatct atcaatgtaa gcataaaaga
     7861 agtttttaat ttttgctgtc caattatgat aacaactttg ttcatttggt ttcaaagctc
     7921 tggatatcga tttgctgtcg agcaaatgtt tggcttaaag tttttaggta tactagctgt
     7981 atgttttgct gtgtcagggc aaataatgtc agtgattgag tctttagtga cacagatatt
     8041 tcaaccaaca ttatttaagc atatggataa tgtcaaaaat agaaagcaac atctagattt
     8101 ttatattaat gaaaatatag caatttatgc tagtgtatta tttttttgta ctttctttat
     8161 gcgcgaaata tttttggttt tagttgacaa taaatatctt ccttacttct atataggtat
     8221 aatagcttta tggagtgaat tttttaggat atcaaccaat actttgtctt tagtgttttt
     8281 ttgcgaaaag aaaatgaagg taggaatttt tccttattta attggctcat taagcctggc
     8341 tttttcgatc gtcttgttca ggttgttaaa tgtaaatttg ggtattttaa tagcttttgc
     8401 tattggggtt gcacaattgg cctcctttat cgtttgctta attacggtaa aaaaaatctc
     8461 ttattttaaa ataaattcca tcttcttaat gaagaggata ataatagtac taccctctgt
     8521 gttaattgcc cattgcattc ctaaatttac atttaacaat gctatccatt ttattatcat
     8581 tttcggtgtc ggtttatttt acttaatctc tttagttact tcttttatgt ttattaaaca
     8641 accaggaggt gtttatggaa gttaaaatta ttggggggct tggcaatcag atgtttcagt
     8701 atgcaactgc atttgctatt gcaaaaagaa ctcatcaaaa tttgactgtt gatatatctg
     8761 atgcagtaaa atataaaact catccattaa gattagttga attatcttgt agttcagaat
     8821 ttgtaaaaaa agcatggcca tttgaaaaat acttattttc cgaaaaaatc ccacatttca
     8881 tgaaaaaagg aatgttcaga aagcattacg ttgagaaaag tttagagtat gatccagata
     8941 ttgataccaa aagtataaat aaaaaaatcg tcggttattt tcaaactgaa aaatatttta
     9001 aggaatttag gcatgagttg ataaaggaat ttcaacctaa aaccaagttt aatagttatc
     9061 aaaatgaatt gcttaattta attaaagaaa atgatacttg ttcattacat attagaagag
     9121 gtgattatgt ttccagtaaa atagcaaatg aaactcatgg cacatgtagt gaaaaatatt
     9181 ttgaacgtgc cattgattat ttgatgaata aaggtgttat taataacaaa acccttctct
     9241 tcattttttc tgatgatata aagtggtgca gggaaaatat attttttaat aatcaaatat
     9301 gttttgttca aggtgatgct tatcatgttg aattagatat gttgcttatg agtaaatgta
     9361 aaaataatat aatttcaaat agttctttca gttggtgggc tgcttggtta aatgaaaata
     9421 aaaataaaac agtaattgct ccgagtaaat ggtttaagaa agatattaaa catgatatta
     9481 ttcccgaatc gtgggtaaaa ttatgatgga agatacaaaa gaattaataa cagtaataat
     9541 gcctgtttat aatgcggaaa aatacatata tgaatctata aaaagtattt tggtgcagtc
     9601 atattctaat tttgagttca tcattattaa tgatggctca aaagattctt ctggaaaaat
     9661 tattgaaaac tttctcgatg atacgaggat acgatatata aatcgtgaaa ataaaggttt
     9721 ggtcttcact ctgaatgaag ctatttcgtt atcaagagga aattatattg ctcgaatgga
     9781 tgctgatgat atttcacatc ccctacgatt agaaaagcaa ctaaattttc tactagaaaa
     9841 tccagatata gccgtagttg ggtgttcttc ttttattatt gaccaaaatt ctaaggttat
     9901 aaatacacga aaaccacctt tgacaccttt agtaaataaa gcattgctat tttttggccc
     9961 aacattaact catccatcag tgatgtttaa taaaatgctg cttggagatc agctttacta
    10021 cagtgataaa tatcttcatg ttgaagatta tgacttgtgg ttgaggttaa ttaatcaata
    10081 caaaatagct aatatcaaag atgttctttt ttactatcga attaatgagt caggtgtttc
    10141 acagactaat ttatttgaac aaaaaattaa tgctgctaaa gcatatagtg aaattaaata
    10201 tgatggtaag tataaagact tactggaaaa gttagaagtc atacacctga gacatgaaat
    10261 ggagaaaagt aaggtctttc aggccgtatt atatatatta ttaaagtatg aacatgattc
    10321 attattattt ttaaaattag gatatatatt acgatggtta ataaaataaa aatagcacta
    10381 ataagtacag aaaaagctaa tcttcctgaa attgaagcct ataaggagta tttttcggag
    10441 ccatttgatg tctctgttgt taataaaaat gatgatttaa gttgttatga tgttttatgg
    10501 tactttatgg ggtttaaagg taataaaata aactctaaac agttcttgat tcatgagttt
    10561 tcctcaattt cagtaccacc atttccaaaa ataaaaaacg ttatcaagaa atttattttg
    10621 cctaaaccta atttgagggt ttttttaaat aaatcagtgc aacaacaatt cgattttaat
    10681 gatagagttc ctttttgtta ccgagatatg ggcgtcaatt ataaaccagt agttgaaaga
    10741 aataataaaa aatatgatat attatatgtg ggggcaatta ctggtagaaa actagatgat
    10801 gcattaaatt tgattttgtc atataaatta gattgtaaaa tcgcaatttt tggtaaagtg
    10861 ccagaaaagt ttaaagaaaa atataaaaat gctgatgtta tatttcttgg gcaagttcct
    10921 tataatcggg tagccaccat tattgcagaa tctaacttgt gtttaaattg gattccgaat
    10981 atttatccat ataacatgca gacatcaaca aaattcttag agtatctggc acaaaataaa
    11041 caaataatat cgaatagcta cccttgggtt gactcttatt gtcaacagca taatattgac
    11101 tatattaata tcaatgattc caattctatt cgacaagcat tgaactcagt cataatccaa
    11161 aaatcaacac atatcccccc aagttggcga gaggttatta tgaatagcgg gatagaaaat
    11221 cacattaaat catattttga ggtaacaaaa tgatttctta cggagtgcta tttctcttct
    11281 cttatatcaa tgctgtactt cagaagagag taaccgtaac attactattt tttataatga
    11341 taattgtttc tttctttaaa tataatattg gatttgattg ggggaattat catgaaattt
    11401 accttcagca aggagatggc tttttttttt atgaacccct attttctctc ttaattattc
    11461 tttccaataa ggttgcatta gaatataaat attttatatc aatgtgctca ttaattacac
    11521 tgattgttta ttattctggt atgcggtgtt ttgcaagaga tataaaagag ttagctttag
    11581 cattaactta ttttgttgtc tcttattgct actttgaaca ctttaatatt attcgacaaa
    11641 ctttagcaat cgctttattc ttttatagtt ggaaatatat tattgagact aactttataa
    11701 tgtatagcct ctttattttt tttgcatcta tgattcatgc ggcggcggtt gtattgctcc
    11761 cattttactt ttttcttaga attgataatt ttaaacctgt atattatggt atagtggtct
    11821 tatttattat tattgttgtt agattcgata ttaacagcat ccttttaagt tcacttaatt
    11881 tatttaaaaa tttcgatttt gtagctaggt atttagcaaa ttcaacagat ttggctgact
    11941 ttgcatcaaa aaaatctgat cttggattaa gtaagatgta cccagcaatt atatttgttt
    12001 tctgttgttt ttggggaggg attaggttag agtctcgaag agactatgtt ttttttaatg
    12061 ctggctttat tgcaataact ttactgctct taagctatca ggttgatatt ttgtttagat
    12121 ttttctcttt ttttgagact tcatttgtct tgttgtactg cattattttt tctggtttta
    12181 ataaaaaaag taaaattata ctgcatatgg ttaatttaat tgtatatacg gtaatgacat
    12241 ttaacatgtt atcagtaatg ggcttgattc cttatgaatc atacattttc taataagctt
    12301 ccctcagttg tttgttgtat agtaatattt taatatcatt gcagtgtgta attattgtca
    12361 ataagctaat ttctgttatt tgcatgatta attcagtttg caatgcttga tgtaaaaaga
    12421 taaagtacac ccgtttacac aatgtttatt ttggcatgtc aagtataagt tctaaatact
    12481 acgtttgtaa tgtttagcat gtaaacataa tagttaaata atattaaaca tgcaaataat
    12541 agaaattatt ccaataaaac atatgtgttg tatgaattat ttaatgtgga aagtggttgc
    12601 gtattataaa ttgttcatat tgacgtttaa gtcttttata actccatgct tgcatgttaa
    12661 aaatatttcg ctatatatta tggtaggtgg tttttatcta ggcagatttg tctatttaac
    12721 tctaaatcta aatcgcttaa gaaagtatga gtaggagtat ttatggctat tctcgttaca
    12781 ggtggtgctg gttatattgc atcacatact atactgagtt tgatagagca gggtaatgaa
    12841 gtaattatta ttgataattt atcaaattca tattatgatt cgttattgaa aataaaagag
    12901 attactggat gtgattgtct tttttataaa ggagatatcc ttgacaaaaa actcttgcta
    12961 aaaatatttg atgaaaataa tatcactgct gtgatgcatt ttgctggtgc aaagtctgta
    13021 agtgaatctg ttatcagacc tttgcaatac tatcgaaata atgtctctgg cactttttct
    13081 ttagttgagg caatggagga gtctggtgta gaaaatttaa tatttagctc ttcagcaact
    13141 gtctatggtg agccaaagat tattcctgtg aatgaaagtt gtgctatagg tggtactaca
    13201 aatccgtatg gaacgtccaa gttatttgta gagaactttt tgcgggatta ttctaaggca
    13261 tcccctaatt ttaaaactat agtattaaga tattttaacc ctatcggtgc tcactcatct
    13321 ggaaaaatag gtgaagatcc taacggaatt ccgaataact tgatgccctt tatatgccag
    13381 gttgctatcg gtaaacaaaa aacgctcaag atctacggaa atgattatcc tactaaagat
    13441 ggtactggga ttcgcgatta tattcatgtt atggatcttg ctgaaggtca tgttgctgca
    13501 ttaaataata ttaatcaagg agctaattat agagtttaca accttggaac aggaattgga
    13561 tactctgtgt tggaattatt agaagcattt cagaaagtta caactcggag agttccctat
    13621 gtttttacta aaaggcgttc aggtgatatt gctgaatgtt ggtctgatcc taccaaagcg
    13681 tatgaagaat taggatggaa agcaaggcga gggttagaag atatgattcg tgatgcatgg
    13741 aattggcagc aaaaaaatcc aaatggattt aagaaagttt aataaaaatg aaacgatata
    13801 ttaagtgtta gcttgtttaa ttaatttaaa gttaatatta tttacattaa tatgtaatga
    13861 aagtgatatt ctatgacagg agtaaacaat gtcaaagcaa cagatcggcg tcgtcggtat
    13921 ggctgtgatg ggacgcaacc tcgcgctt
//
