LOCUS       KP835691               10743 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain 8547 serotype OX43:H28 O-antigen gene
            cluster, complete sequence.
ACCESSION   KP835691
VERSION     KP835691.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10743)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10743)
  AUTHORS   Yan,X., Baranzoni,G.M., Fratamico,P., Debroy,C., Tebbs,R.S.,
            Liu,Y., O'Connell,C.D., Swimley,M. and Matheny,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2015) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
FEATURES             Location/Qualifiers
     source          1..10743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="8547"
                     /serotype="OX43:H28"
                     /db_xref="taxon:562"
     misc_feature    1..10743
                     /note="O-antigen gene cluster"
     gene            108..1193
                     /gene="rmlB"
     CDS             108..1193
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AKM71180.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFERADVCDAAAMARIFALHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLIICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANAKWVGNVKSGAYQSWIAQNYEGRQ"
     gene            1193..2092
                     /gene="rmlD"
     CDS             1193..2092
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AKM71181.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGNGDMPWLETDTTAPLNVYGETKLAGEKALQEHCPKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRMALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTVKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2150..3028
                     /gene="rmlA"
     CDS             2150..3028
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase 1"
                     /protein_id="AKM71182.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMEAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSDYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGEYLLKMIKGY"
     gene            3033..3587
                     /gene="rmlC"
     CDS             3033..3587
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AKM71183.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLDPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSADNKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPDAEGSLRWNDDDININWPSS
                     YGITLSKKDENAPYFNRIIGNGIL"
     gene            3584..4840
                     /gene="wzx"
     CDS             3584..4840
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AKM71184.1"
                     /translation="MSLFKNAGWNILGLVIPLIMAIPVMGIMARLLGVERFGLFTIAY
                     ALMGYASVFDGGISRAVIRFVSIYRNDNEKVNVILGSSIYTVTVLSILIALLTYLVSP
                     NLVVFLKVSQDIRHEVVIALQLLGACIPALLLTQVWRAYLEGVENFSSLNIQQVLTSP
                     FIILLPLTTTWFSQTLISAVLGLILGRWIVAFISWKLCSRHLKNFKRTFSRKALMELI
                     SFGGWVTLSNIIGPLMGFIDRFAVSHFAGANVVAYYSGPSDMVSRLTLFPGAVARALF
                     PKLSFETREESKVTYFRSLILVFCLSFLLALPFFLFSSDILGIWLGNEYRGDAAVVFR
                     ILLIGFVFNSIAQICFARIQSAGFAKWTGIVHIMEFIPYLVLLYLLTVKYSFFGAAFA
                     FSLRSLLDFIILFLVERIGIYNMDRI"
     gene            4842..5966
                     /gene="wzy"
     CDS             4842..5966
                     /codon_start=1
                     /transl_table=11
                     /gene="wzy"
                     /product="polymerase"
                     /protein_id="AKM71185.1"
                     /translation="MNVVQAMVIIYLCYLIICFFFRLEITHPAVVHTIIWLISASLAL
                     VFAEEKYLPNNYYIVIANSMFFIFSFIGGIGVFNKNKAFQLNIDKNSYVNALPFCLII
                     SFYLIILITYNVNVLSNISAFRDYLVADDGANYGLLGRIAMLSLFSSCFLLLRSRRLF
                     LLSAVLCIPIIIILSAKTLVLLYLITILILTPNRLKISKLLIFFSIFILFFIGIMRLR
                     YPEASFNLILYYLYNYISGGFLAFSQLPNSFNQVFGFYSFRNFYLWLNVLYPVEIANI
                     LQEWVNVPFPVNVYTYLRPYYMDFDYLSLFFPIIFGFFSGRIYMQKYKNRRIYYIVYP
                     ITFYAIAMQLFDDQYLTWLSNWILLIITGYIMTWEGVCRK"
     CDS             5954..6856
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="glycosyltransferase"
                     /protein_id="AKM71186.1"
                     /translation="MSKIAVLIVTYNPVLSVLDDLIGKIKKAKYNLEIFLVDNASENA
                     NILKAKFKDEPNILFLDENLGLAGAQNVGLKKILRTDCEAVLFFDQDTSPTDVFFTNL
                     IESMSKLALQGEKIGAIGPVFYDSRTGTKYPFILLKRIRAKKVFPTGNVPLKVSFLIN
                     SGMLVPVSTLKDVGLMKEELFIDYVDIEWCLRAASKKFNFYAIPDATMSHALGDERKS
                     ILGREISIHSPLRRYYLARNSIYMLRLPYVPLGYKIREILFSLLRTAIFISTVNNKKV
                     YVDFILKGWSDGIKKNYGKYVGIK"
     CDS             6904..7785
                     /codon_start=1
                     /transl_table=11
                     /gene="orf8"
                     /product="glycosyltransferase"
                     /protein_id="AKM71187.1"
                     /translation="MEKKVISSVTVTYNPDIECLRQQLLSVRKQVDLCVIVDNGSNNI
                     IEIKKLNYEFDFYLIQFDDNYGLAYAQNSGIEYAISSGTEYLLLLDQDSILKDEFSKN
                     MKHIYIKYNVGILGPSFYDPQNNKIYFGTCYKGPFISRKSIGEVTDVTFVIASGSFFS
                     SDVYKKIGGMKSELFVDYIDVEWSLRAKNMGYRVCMTNRALMAHTIGDSRITIFGRTI
                     SVHSPIRRYYLVRNSFYMLRLHYVPIGYKVREIFFNCLRSIISLIISDKKGNVITKLG
                     QGIYDGIRGKYGDYKDR"
     gene            8134..9270
                     /gene="yhhI"
     CDS             8134..9270
                     /gene="yhhI"
                     /codon_start=1
                     /transl_table=11
                     /product="H repeat-associated protein"
                     /protein_id="AKM71188.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLTDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSND
                     KDVIAIDGKTLRHSYDKNRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVINEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            9503..10594
                     /gene="wbbG"
     CDS             9503..10594
                     /gene="wbbG"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 1"
                     /protein_id="AKM71190.1"
                     /translation="MKKIIHIQVLPKMSGVQQVSFDILSGINDENVQKFILCGSPDGS
                     SDDDFKQKFTDIGVRVITMPSLKRNIGWHDFRCFIDLYNFFKKEKFDIVHTNSTKPGI
                     IARIAARLAGTKLIIHTVHGIAFHRKENTVRKIFYYCLENFATLFGSINVTVNENYLK
                     YYPFVKSHIVYNGVDFNVLCCNKKDHDFLHIAFMARLDKQKNPLEFIRAVNIIKKKLP
                     NERLKFTLAGCGELENECKKLIEHFHLTDVIDMPGWIVDKNTFYNSVDIICQPSNWEA
                     FGLVFVEAAFFEIPSVSRNIEGIPEVILDNETGLLYEGGEAELSEKLISLIHDKKKIS
                     WLGLNAKEYVLKHFTKDIMVEKYSKLYDL"
BASE COUNT     3301 a   1698 c   2245 g   3499 t
ORIGIN
        1 ctctggtagc tgtaaagcca ggggcggtag cgtgcattaa tacctctatt aatcaagcta
       61 agagccgctt atttacagta tgctctgaag taatatggaa taaaaaagtg aagatacttg
      121 ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata aataatacgc
      181 aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctcgaa tcacttgctg
      241 atgtttctga ttctgaacgc tatgtttttg aacgtgcgga tgtttgcgat gcagctgcaa
      301 tggcgcggat ttttgctctg catcagccgg atgcagtgat gcacttggct gctgaaagcc
      361 atgtggatcg ttccattaca ggccctgcgg catttattga aaccaatatt gttggtactt
      421 atgtcctttt ggaagccgct cgcaattact ggtctgctct tgatagcgac aagaaaaata
      481 gcttccgttt tcatcatatt tctactgacg aagtctatgg cgatttgcct catcctgacg
      541 aagtaaataa taaagaagaa ttacccctct ttactgagac gacagcttac gcgccaagca
      601 gcccatattc tgcttctaaa gcatccagcg atcatttagt ccgcgcgtgg aaacgtacct
      661 atggtttacc gaccattgtg actaactgtt cgaataacta cggcccttat cactttccgg
      721 aaaaattgat tccgctggta attcttaatg ctctggaagg taaggcatta cctatttatg
      781 gcaaagggga tcaaattcgt gactggctgt atgttgaaga tcatgcgcgt gcgttatata
      841 ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtggacac aacgaaaaga
      901 aaaacatcga tgtagtgctc attatttgtg atttgttgga tgagattgta ccgaaagaga
      961 aatcttaccg cgagcaaatt acttatgttg ccgatcgtcc gggacacgat cgccgttatg
     1021 cgattgatgc tgacaagatt agccgcgaat tgggttggaa accacaggaa acgtttgaga
     1081 gcgggattcg taaaacggtg gaatggtacc tggctaatgc aaaatgggtc ggaaatgtga
     1141 aaagtggtgc ctatcagtca tggattgcac agaactatga gggtcgccag taatgaatat
     1201 cctccttttc ggcaaaacag ggcaggtagg ttgggaacta cagcgtgctc tggcacctct
     1261 gggtaatttg attgctcttg atgtccactc cactgattat tgtggtgatt ttagtaaccc
     1321 tgaaggggtg gctgaaacag tcaaaagaat tcgacctgat gttattgtta atgctgcggc
     1381 tcataccgca gtagataagg ctgagtcaga acccgaattt gcacaattac tcaatgcaac
     1441 tagcgttgaa tcaattgcaa aagcggctaa tgaagttggg gcttgggtaa ttcattactc
     1501 aactgactac gtattccctg gaaatggtga catgccatgg ctggagacgg atacaaccgc
     1561 tccgctgaat gtttatggtg aaactaagtt agccggagaa aaagcattac aagaacattg
     1621 tcctaagcac cttattttcc ggactagctg ggtctatgca ggcaaaggaa ataacttcgc
     1681 gaaaacgatg ttacgtctgg caaaagagcg cgaagaatta gcggttatta acgatcagtt
     1741 tggtgcacca acaggtgctg aactgctggc tgattgtacg gcacatgcaa ttcgtatggc
     1801 actgaataaa ccagaagtcg caggtttgta ccatctggta gccagtggta ccacaacctg
     1861 gcacgattat gctgcgctgg tttttgaaga ggcacgaaaa gcaggtattc cccttgcact
     1921 caacaagctc aacgcggtac caacaacagc ttatcctaca ccagctcgtc gtccacataa
     1981 ctctcgtctt aatacagtaa aatttcagca gaactttgcg cttgtcttgc ctgactggca
     2041 ggttggcgtg aaacgaatgc tcaacgaatt atttacgact acagcaattt aatagttttt
     2101 gcatcttgtt cgtgatggtg gagcaagatg aattaaaagg aatgatgaaa tgaaaacgcg
     2161 taaaggtatt attttagcgg gtggttctgg tactcgtctt tatcctgtaa ctatggctgt
     2221 cagtaaacag ctgttaccga tttatgataa accgatgatc tattatccgt tgtctacact
     2281 gatgttagcg ggtattcgcg atattctgat tatcagtacg ccacaggata ctcctcgttt
     2341 tcaacagttg ctgggtgatg gcagccagtg gggcctgaat cttcaataca aagtgcaacc
     2401 gagcccagac ggtcttgcgc aagcatttat catcggtgaa gagtttatcg gtggtgatga
     2461 ttgtgctttg gttcttggtg ataatatctt ctacggtcac gatctgccga agttaatgga
     2521 agcagctgtt aacaaagaaa gtggcgcaac ggtatttgct tatcacgtaa atgatcctga
     2581 acgctacggt gtcgttgagt ttgataaaaa cggtacggca attagcctgg aagaaaaacc
     2641 gctacagcca aaaagtgatt atgcggtgac tgggctttat ttctatgata acgacgttgt
     2701 cgaaatggcg aaaaacctta agccatctgc acgtggtgaa ctggaaatta ccgatattaa
     2761 ccgtatttat atggaacaag ggcgtttgtc tgttgctatg atgggccgtg gttatgcatg
     2821 gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg caacaattga
     2881 agagcgtcag ggactgaaag tttcctgccc ggaagaaatt gcttaccgta aagggtttat
     2941 tgatgctgag caggtgaaag tattggctga accgttgaag aaaaatgctt atggtgagta
     3001 cctgctaaaa atgattaaag gttattaata aaatgaacgt aattaaaaca gaaattccag
     3061 atgtattaat tttcgagccg aaagtttttg gtgatgaacg tggtttcttt tttgagagct
     3121 ttaaccagaa ggtttttgag gaagctgtag gccgcaaagt tgaatttgtt caggataacc
     3181 attcgaagtc cagtaaaggt gttttacgcg ggctacatta tcagttagac ccttatgcac
     3241 aaggaaaatt ggtgcgctgc gtagttggtg aagtttttga tgtagcggtt gatattcgta
     3301 aatcgtcacc tacatttggg aaatgggttg gggtaaattt gtctgctgat aataagcgcc
     3361 agttgtggat cccggaaggg tttgcgcatg gatttttggt gttgagtgaa acggctgagt
     3421 ttttgtataa gacgacaaat tattatcatc ctgatgctga aggttcatta cgttggaatg
     3481 atgatgatat taatattaat tggccatcat catacggtat cactctatca aagaaggatg
     3541 aaaatgcacc gtacttcaac agaataatcg gtaatggaat tttatgagtt tatttaaaaa
     3601 cgctggctgg aatattttag gtctggttat tccacttatt atggcgatcc ccgttatggg
     3661 gatcatggct cgtttattag gtgttgaacg ctttggtctt ttcactattg cttatgcttt
     3721 aatgggatat gcaagtgttt ttgatggcgg aatatctcgt gcagttattc ggtttgtatc
     3781 tatttataga aatgacaatg aaaaagttaa tgttatttta ggttcttcta tatatacggt
     3841 aactgtttta agtatcctaa tagcattatt aacttattta gtatcaccaa atttggttgt
     3901 ttttttaaaa gtatcacagg atattcgaca tgaagttgta atagctcttc agctactggg
     3961 ggcatgtatc cctgcattac tacttacaca agtatggcga gcatatcttg aaggtgtcga
     4021 aaatttttct tctttgaaca ttcaacaagt tctaacaagt cctttcataa tattattacc
     4081 attaaccaca acatggtttt cacaaactct tatttcagca gtattagggt tgattttggg
     4141 acgctggatt gttgcattta ttagttggaa actttgttcg cgccatctta aaaatttcaa
     4201 aagaacattt agtcgaaaag ctttgatgga gcttattagc tttggtggat gggtaactct
     4261 gagtaatatt ataggccctt taatggggtt tattgatcgt tttgctgtct cacatttcgc
     4321 tggcgctaat gttgttgctt actattcagg gccgtctgat atggtatctc gacttacttt
     4381 atttcctggg gctgttgcca gggctctatt tccaaaacta agttttgaaa ctagagaaga
     4441 gagcaaagtt acatattttc gatctctaat tctcgttttt tgtttatctt ttcttttagc
     4501 attgccattt ttcttatttt caagcgatat attaggtata tggttaggaa atgagtatag
     4561 aggagatgca gcggtcgtat ttcgcatatt gttaattgga tttgttttta attcaatagc
     4621 acaaatatgt tttgctagaa ttcagtctgc tggatttgcg aaatggactg gtattgtgca
     4681 tattatggaa ttcataccat acttggttct tttgtattta ctgaccgtca aatattcttt
     4741 ttttggagct gcgttcgcct tttcattaag gtcattattg gattttataa ttctattcct
     4801 ggttgaaagg atcggtatct ataacatgga taggatataa tatgaatgtc gttcaagcaa
     4861 tggttataat atacctttgt tacctaatta tatgtttctt ctttaggctt gaaataacac
     4921 atccagcagt tgtacatacg ataatttggc ttataagcgc gagcttagct cttgtttttg
     4981 ctgaagaaaa atatttgcca aataactatt atatagttat agcaaactca atgtttttta
     5041 ttttttcttt cattggtgga attggtgttt ttaataaaaa taaagctttt cagttaaata
     5101 ttgataaaaa ttcttatgta aatgctttac cattttgttt aataatatca ttttatttga
     5161 ttatactaat aacctataat gtgaatgttt tatcaaacat atcagcgttc cgagattatc
     5221 tggttgctga cgatggtgct aattatggtt tattagggcg tatcgcaatg ttatcgctct
     5281 tctcttcttg ttttttactt ttgagaagtc gaaggctttt tttacttagc gccgtgttgt
     5341 gcattcctat aattattata ctaagtgcaa aaaccttagt tctattgtat ttaataacga
     5401 tactaatttt aactccaaac aggctgaaaa tatcaaaact actaatcttc tttagcatat
     5461 ttattctttt ctttattggg ataatgaggc ttcgatatcc agaagcatct tttaatttaa
     5521 ttttatatta tttatataat tatatttcag gtggattttt ggctttttca caattgccga
     5581 attcatttaa tcaagttttt ggtttttatt ctttcagaaa cttttacctt tggttaaatg
     5641 tgctctatcc agtcgagatc gcaaacattc ttcaagagtg ggtaaatgtc ccatttcctg
     5701 tcaatgtata tacgtatctt cgcccttatt atatggattt tgattattta tcattatttt
     5761 tcccaattat ctttggcttt ttttcgggaa gaatatatat gcaaaagtat aaaaatagac
     5821 gcatttatta tattgtatac ccgataacct tttacgcaat agcaatgcag ctatttgatg
     5881 atcaatatct tacatggtta tcaaactgga tattgcttat aattacaggc tatataatga
     5941 cttgggaggg ggtatgtcga aaatagcagt tttgatagta acgtataatc cagttttaag
     6001 tgtgttggat gatctaattg gaaagataaa aaaagctaaa tataatttag aaatatttct
     6061 cgttgataat gctagtgaaa atgctaatat attaaaagct aaatttaagg acgagcccaa
     6121 tatattattc cttgatgaaa atttgggatt ggctggggca caaaatgtag gtctgaaaaa
     6181 aatattacga acagactgtg aagcagtcct tttttttgat caagatactt cccctacaga
     6241 tgtatttttt actaatttga tagagtctat gtcaaagtta gctctacagg gagaaaaaat
     6301 tggtgctatt ggacctgttt tttacgactc aaggacagga accaaatatc catttattct
     6361 cttaaaaaga atccgagcta agaaggtttt tccaacaggt aatgtcccgc ttaaagtttc
     6421 atttttaata aactcaggta tgctagtacc agtaagcaca ttaaaagatg ttgggttaat
     6481 gaaagaggaa ttatttatag actatgtcga tattgaatgg tgcctccgag ctgcaagtaa
     6541 aaagtttaac ttttacgcta ttcccgatgc aacgatgagt catgcattag gagatgagcg
     6601 taaatcaatt ttgggaagag aaattagcat acacagccct ttgagacgtt attatttagc
     6661 aagaaattct atttatatgc tgcgtttacc atacgttcct ttggggtata aaataagaga
     6721 aattttgttt tcattgttga gaacagcgat atttatatca actgttaata ataagaaggt
     6781 ttatgttgat tttattctaa aaggttggtc ggatggcatt aaaaaaaatt atgggaagta
     6841 tgttggaata aagtagatat tttttttgaa acgaataaac ttggacaaat ttaatccctt
     6901 tcaatggaga aaaaagtgat tagttcggtt acagttacat ataacccaga tattgaatgc
     6961 ctcagacagc aacttttatc agtacgtaag caggttgatt tatgtgtaat tgttgataat
     7021 ggctcaaaca atattattga gattaaaaaa ttaaattatg agtttgattt ttatcttatt
     7081 caatttgatg ataattatgg tctcgcatat gcacaaaata gtggtataga atatgccata
     7141 tcttctggta cagaatattt attattgttg gatcaagata gtatcttgaa agatgaattt
     7201 tctaagaaca tgaagcatat atatataaaa tataatgttg ggattttagg accttcattt
     7261 tatgatcctc aaaacaataa aatttatttt gggacttgtt ataaaggacc ttttattagc
     7321 cgtaagtcta tcggtgaggt taccgatgtt acttttgtca ttgcatcggg ctcctttttt
     7381 tcatcagatg tttataaaaa aattggagga atgaaatctg aactttttgt ggattatatt
     7441 gatgttgaat ggtctcttag agcaaaaaat atgggataca gagtgtgtat gactaacagg
     7501 gctttaatgg ctcatacaat tggtgattcc agaattacaa tttttgggcg gacaatatcg
     7561 gtgcatagcc ccataagacg ttattattta gttagaaata gtttctatat gctaaggttg
     7621 cactatgtgc ctattggata taaagttcgc gaaattttct ttaattgttt acgatcaata
     7681 atttctctaa taataagtga caaaaagggt aatgtaataa ctaaactagg tcaaggaata
     7741 tatgacggta ttcgtgggaa atatggtgat tataaagata gataatatta attcatccgt
     7801 tgcgtcacaa ttaaattaga attcatgtac aaattaatac aaaggaaaag taatacttaa
     7861 agtatattca acactaaata attcactttt cctatcatgg aaagatcatc ggtgtgctgt
     7921 aggcacaaag atgcaaaacg ggacgttttg catgtaataa gcataagctt gtaaagagat
     7981 ggtggcaggc agggaaagat catcagaagt tcactttttg tactaaataa ttcgcatttt
     8041 atgtttaaaa attgagatat tccttattac ctaaagctgt tttttattgc ttatacatga
     8101 tcaaatactc cttacataat taaggagaac aaaatggaac ttaaaaagtt gatggaacat
     8161 atttctatta tccccgatta cagacaagcc tggaaagtag aacataaatt gacagatatt
     8221 ctactgttga ctatttgcgc cgtcatttct ggtgcagaag gctgggaaga tatagaggat
     8281 tttggtgaaa cacatctcga ttttttgaag caatatggtg attttgaaaa tggtattcct
     8341 gtccacgata ccattgccag agttgtatcc tgtatcagtc ctgcaaagtt tcacgagtgc
     8401 tttattaact ggatgcgtga ctgccattcc tcaaatgata aagacgttat tgcaattgat
     8461 ggaaaaacgc tccggcactc ttatgacaag aatcgccgca agggagcgat tcatgtcatt
     8521 agtgcgttct caacaatgca cagtctggtt ctcgggcaga ttaagacgga tgagaaatcc
     8581 aatgagatta cagccattcc tgaacttctt aacatgctgg atattaaagg aaaaatcatc
     8641 acaactgatg cgatggggtg ccagaaagat attgcagaga agatacaaaa acagggcggt
     8701 gattatttat tcgctgtaaa aggaaaccag gggcggctta ataaagcctt tgaggaaaaa
     8761 tttccgctga aagaattaaa taatccagag catgacagtt acgctattag tgaaaagagt
     8821 cacggcagag aagaaatccg tcttcatatt gtttgcgatg tccctgatga acttattgat
     8881 ttcacgtttg aatggaaagg gctgaagaaa ttatgcgtgg cagtctcctt tcggtccata
     8941 atagcagaac aaaagaaaga gccagaaatg acggtcagat attatatcag ttctgctgat
     9001 ttaaccgcag agaagttcgc cacagcaatc cgaaaccact ggcacgtgga gaataagctg
     9061 cactggcgtc tggacgtggt aataaatgaa gacgactgca aaataagaag aggaaatgca
     9121 gcagaattat tttcagggat acggcacatc gctattaata ttttgacgaa tgataaggta
     9181 ttcaaggcag ggttaagacg taagatgcga aaagcagcca tggacagaaa ctatctggcg
     9241 tcagtccttg cggggagcgg gctttcgtaa tctcgccctg acatggacct ctaaggcgat
     9301 attaattgtt tgaaaattat atctcaaatt taaaaatgag ctgtatgctg ttagagtata
     9361 aaatatgtgt ttttatattg gaaggttttt agtgagcatg attttaacca aaaatataaa
     9421 tactttactt tcatacacta taacatcaat taaggatgga ataaatggtg aaacggggga
     9481 atataaagga taacaactca ttatgaaaaa aataatacat atccaagtgt tgccaaaaat
     9541 gtctggggtt cagcaagtct cttttgatat tttgtcaggg attaatgacg aaaatgtaca
     9601 aaaatttata ttatgtggtt caccagatgg cagttcagat gatgatttta aacagaaatt
     9661 tactgatata ggtgttaggg ttattacgat gccttcatta aaacgaaata tcgggtggca
     9721 tgattttcga tgtttcattg atttatacaa tttctttaaa aaggaaaagt ttgatatagt
     9781 tcatacaaac tcaactaagc caggaataat agctagaata gccgctagat tagctgggac
     9841 aaaactcatt attcacacag tacatggaat cgcatttcat agaaaagaaa atactgtaag
     9901 gaaaattttt tactattgcc ttgaaaattt tgcaacactt tttggtagca ttaatgtgac
     9961 agtgaacgag aattatttaa aatattatcc attcgttaaa agtcacattg tatataatgg
    10021 agttgatttc aacgttcttt gctgcaataa aaaggatcat gattttttac atattgcatt
    10081 tatggctaga cttgataaac aaaaaaaccc tttggagttt ataagagccg ttaatattat
    10141 taagaaaaaa ttaccaaatg agcgtttgaa atttacattg gctggctgtg gtgagttaga
    10201 aaatgaatgt aaaaaattaa tagaacattt tcatcttaca gatgttattg atatgcctgg
    10261 atggatagta gataaaaaca cattttataa ctctgtcgat attatttgcc agccatccaa
    10321 ttgggaggct tttggcttag tatttgttga ggccgcattt tttgaaattc catctgtttc
    10381 aaggaatatt gaagggattc ctgaggttat tttagataat gaaactgggc ttttgtatga
    10441 gggtggagaa gccgagttga gtgaaaagct gatatcatta attcatgata aaaagaaaat
    10501 ttcttggtta ggtttgaatg ctaaagaata tgtcttaaag catttcacca aagatatcat
    10561 ggttgaaaaa tattcaaaac tgtatgattt ataagtatct aattaaattt cactatgatt
    10621 tttgcattat gatattatag acccgtgtat tttttcaagc cgcacgccct cgcggtgacc
    10681 accccctgac aggagtaaac aatgtcaaag caacagatcg gcgtagtcgg tatggcagtg
    10741 atg
//
