LOCUS       KJ778794               12491 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain 244-54 serotype OX6:H? O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778794
VERSION     KJ778794.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12491)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12491)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="244-54"
                     /serotype="OX6:H?"
                     /db_xref="taxon:562"
     misc_feature    1..12491
                     /note="O-antigen gene cluster"
     gene            99..1334
                     /gene="wzx"
     CDS             99..1334
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AIG62691.1"
                     /translation="MKQILISFSFRLMGPVIMLLTTPQLLSTLGNVEYGVWSLLITTI
                     SWLTICDFGISNQIRNESKILIVQDNKESIREIYTAAVIISFFITLLCCVIISFINID
                     LIYKLFLSCVFLFAIETNICRALCLSLNKPELASAVTNIPNLFLLIYVFCYSSFNINN
                     VMDIYCYGSAMMFVSIFIILYKFNIKLFWPVFHPSYLRFSIMKYSLLRGGGGFFMVQL
                     VSMGLIISDRYIISSIFGVEDVTKYDLIFKVSNFCIMFFSIVNMVLWSKFTEFWMQGF
                     YTKIKVIFKVFDLCFLLLVIVLILILNHIHWIFELWLNVTNENYNEVYITGIVLYLVG
                     YYWMSAYSSFLNGISIIKKQFIIQGGGLLIKAITLLIAMNILKVFKVEYIILLGGACL
                     FIVGFLFRKCAHDKINSCI"
     CDS             1309..1914
                     /codon_start=1
                     /transl_table=11
                     /gene="orf2"
                     /product="acetyltransferase"
                     /protein_id="AIG62692.1"
                     /translation="MIKSIVVYSFRIYYFINRWITTKINKLRLKANSVELGDSVMING
                     ILHIVNKGEIKIGNGVKINSSLRYNPISHHPCSFYTSENAVIEIGDKSGLSGCTLYSV
                     KKIHIGKRVLIGAGVNIYDTDFHSLLVDERLNGDSNIKSSPVYIYDDCFIGANVTILK
                     GVTIGAGSVVAACSVVTKNIGENELWAGNPARYIRDTIYGE"
     CDS             1856..2632
                     /codon_start=1
                     /transl_table=11
                     /gene="orf3"
                     /product="glycosyltransferase"
                     /protein_id="AIG62693.1"
                     /translation="MNYGQAIQLGILEILYMANDKITVITINYNNAEGLSKTIASVEK
                     QTNKEFEYLIIDGGSEDNSIEHAKKSVICTKIISEKDNGIYDAMNKGASLAKGEYIIF
                     TNSGDELRKNSIEILMNVLRETNTLYDIYHGILAFKKNDKVIYYRGRKSDMLNSSMIE
                     HPTVLMRKSTFNELKGFDNKWRYVADYDLMLRAYFTKRRFFFIEEVIADFDVSGVSSV
                     SIKAPLESLRLQRKYNLINTFEYFSKLFFIKMKKAVSCLR"
     gene            2620..3735
                     /gene="tuaC"
     CDS             2620..3735
                     /gene="tuaC"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AIG62694.1"
                     /translation="MLKVILLSSINPEQKTGYYNAVNDRVKSLKKEKGIKIWSYSIYK
                     STSLIRFSIKKNENHFFIEYPKAFPSHGLFSFITLWFIRCVILFLWVIIRPNMIHVHW
                     GYPLAYAAVIIKRLTKKTKVIVTFHGSDVHTHPARSKEIFVKTCNLCKRVDHVTAVSE
                     NLLLQVRNKFSVSESKSSITYNGVNLDETNCSFSSSNGLTFSFFGNLNMTKGADRIIP
                     LYNALSGLIEENFTFYVGGEGPCYKEIKQYIIVNGISNIKLLGFMPRADVKRIMRKSN
                     CVFILSRNEGLGISAIEALAYSQLCIAPDIGGLSEVFRENPELLIKNDFNPSDYANKF
                     ISCHKDKFRVNRTYIFKNFSMKNIIQKEITLYNEIMK"
     gene            3751..4983
                     /gene="wzy"
     CDS             3751..4983
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AIG62695.1"
                     /translation="MNCKFNIWDIQLGLILISVLSYAFLNLGFLPLNISHLIILFVLI
                     TLSVNLLISQSIKITGLFLTLFVSFILLSAYSLLKYYDVQKVKNIINFFIFFSVAAIV
                     INNCSADKIIKFYYKLTKIFIFFALLQWVLYYLNIGTLYTYSFLGLKEVNISTSGYLI
                     RLFSIASEPAALCGILLPAIYLSINRIVNKGKEVTLSYSVIVLFVILNTFSLVGYIYI
                     IICLIVALYVGNKISLSKIFIFTICLALLVFILFQSDSIQQRLNEITSLDKMASSDNL
                     SVIAIYSNLQIALISLSDNLIFGGGIFSHPYTYDHYISQLYAGGGPRMELNKDDAASL
                     YIRALSETGILGFCILNGLIIYLMKRCDKSKINYPYNIAFTIAFALLGIRAGSVNYII
                     IWFYFFSAIRFVNEGRLK"
     gene            4980..6101
                     /gene="gumH"
     CDS             4980..6101
                     /gene="gumH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AIG62696.1"
                     /translation="MKMLSFLECMNSTYGGPAFSLPSLLHHLEKNYLVQSTVISLKKK
                     NEVYENNVAECLGINPIILNQVGPRKLKLTHEIAKVKKIMQDCDIIHTNNLWNYFSYL
                     PYNLSKNSKKPNVISVRGSLYAWSLEQSRLLKKISWFLFQKKALDSAGFIHVTCNDEL
                     TALRELGIKNKIVVSQHGVDVPDLIDKVPDSFYDKYKLSHNKKYFLFMSRLHTKKGLD
                     MLFNVWSRISHHYPDWELLVAGPDYGNYIPKITKLNAVKYMGMLSGEEKEYCFSIAQF
                     FVLPSYTENFGVVIGEAMARTVPVLTSTNTPWKDVNTNNAGVCFDLSTENLKRELEFF
                     FNLSDSTRKEMGRNARELIVRNYSWERVSYPFYDALKSL"
     gene            6113..7231
                     /gene="gmd"
     CDS             6113..7231
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="AIG62697.1"
                     /translation="MKKALITGITGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERVD
                     HIYQDRHNQNPNFFLHYGDLTDTSNLIRLIKEIQPDEVYNLGAQSHVAVSFESPEYTA
                     DVDAMGTLRLLEAIRICGLEKKTRFYQASTSELFGLVQEIPQRETTPFYPRSPYAVAK
                     MYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLYLG
                     NMDSLRDWGHAKDYVRMQWMMLQQDHPEDFVIATGKQISVREFVRMSAKEVGLELEFS
                     GQGVDEIATVVNKTSDCAIGVSIGDVIVRVDPRYFRPAEVETLLGDPAKAKKVLGWEP
                     EITVEEMCAEMVASDLAKAKQHALLKSHGYDVAVSLER"
     gene            7235..8200
                     /gene="fcl"
     CDS             7235..8200
                     /gene="fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="AIG62698.1"
                     /translation="MTKKRIYVAGHRGMVGSAICRQLSLRDDIELVVKTHKELDLTVQ
                     KEVDAFFEQEKIDQIYLAAAKVGGIYANNTFPAEFIYQNLMIESNIIHSAHKAGIQKL
                     LFLGSSCIYPKFAEQPMNESALLTGILEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGINDNFHPENSHVIPALMRRFHEAKESGAPEVIVWGTGTPMREFLYVDDMA
                     AASVHVMELDEAIYQQNTQPMLSHINVGTGVDCSIREMAETMASVVGYQGKIVFDVTK
                     PDGTPRKLMDVTRLKNLGWQYQYNLHEGLSLTYKWFIENINSFRG"
     gene            8203..8664
                     /gene="gmm"
     CDS             8203..8664
                     /gene="gmm"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="AIG62699.1"
                     /translation="MNKRLERELFKTIVEHTPLISIDLIIRNDKGEALLGQRLNRPAQ
                     NYWFVPGGRIYKDESFEIAFKRITFEEVGVQISLNDALFLGVYEHFYNDNFSEAEFST
                     HYVVHGYEIQLKPQQLHLPTVQHNSYKWFDVVTLLNSTTVHQYTKNYFIPR"
     gene            8670..10076
                     /gene="manC"
     CDS             8670..10076
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AIG62700.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLNSRLTMLQETLRRL
                     DKVEHKPALVICNESHRFIVAEQLRKEGLKHSGILLEPVGRNTAPAVALAALQAMVTG
                     DDPILLVLAADHEIQNEDNFIDAVLAAKNFAEQGKLVTFGIVPTSPETGYGYIKSGEY
                     LDGKGYKVAAFVEKPELHVAQQYISDGGYLWNSGMFMFRASVFIDELKKFRPDILASC
                     QRSLSSSIQDLDFIRLDNASFSCCPEESIDYAVMEKTAEAVVVPLNAQWSDVGSWSAL
                     WEISSKDQSGNAIRGDVLVEDATDSYLYSQHRLIGAVGIKDLVVVETKDAVLVAHKDK
                     VQQVKNIVAQLKKNNRTEYLQHREIFRPWGSHDTIAEGPRFQVKHVIVLPGHITAKQI
                     HYHRTEHWIVVSGTAKVHLEDKTYLVSENESTYIPVGVPHAIENPGKIPLEIIEVRSG
                     VYLEEDDVIRVSSSGVGY"
     CDS             10076..10822
                     /codon_start=1
                     /transl_table=11
                     /gene="orf11"
                     /product="glycosyltransferase"
                     /protein_id="AIG62701.1"
                     /translation="MRISIITATYNSEKTLLDTLLSLEKQTYPDIEYIVVDGASNDNT
                     IKLIKSNCTKVSKIICEPDKGIYDALNKGIQAASGDVIGFLHSDDLLAYDDAIADIAK
                     TFEITGCDAIYGDLEYVAQNDTTKRIRLWKSGSFSRFKMKVGWMPPHPSFYMKRKCYR
                     QFGCFSLDYRISADYDSLLRYILKQRISIEYLPKVLVKMRVGGISNRSVSSMVKKSME
                     DIRVMNQNGIIWPIALVYKNISKLPQFIKK"
     gene            10828..12252
                     /gene="manB"
     CDS             10828..12252
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="AIG62702.1"
                     /translation="MLNVKKIINDSNIAFGTSGARGLVIDFSHDVCAAFTHAFLSVID
                     DKYNFNKVALAIDNRPSSYEIAQACAFAIKQHGFTVEYHGVIPTPALAHYSMQKNIPC
                     IMVTGSHIPFDRNGLKFYRPDGEITKEDELAIINSEYTFPPVGVLPHLETSTQGADYY
                     LERYVSLFNPEILKGKRIGVYEHSSAGRDLYAPLFNQMGAEVISLGRSDEFVPIDTEA
                     VSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKA
                     LAIPVSCNTAIEESNKFASIQRTKIGSPYVIAAFADLAKQFDSVAGFEANGGFLLASD
                     LQINGKELKSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRVKNFPSDSSQ
                     QIIKNAISSPNNFFNSLGYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPELRCYA
                     EASDENQAKQYVTNVLGNITSLIS"
BASE COUNT     3960 a   1889 c   2441 g   4201 t
ORIGIN
        1 agaatctacg cagtgcactg gtagctgtta agccaggggc ggtagcgtgt gcaaataaga
       61 atatgttccg acaaataaat ttcaaaaatc tagaaataat gaaacagatt ctaatttcat
      121 tttcttttag attaatgggt cctgttatca tgcttctgac cacgccacaa ttgttgtcga
      181 ctttgggaaa tgtggaatat ggagtttggt ctttattgat tacaacgatt agttggttga
      241 caatatgcga ctttggcata tcaaatcaga tacgaaatga aagtaagatt ttaattgttc
      301 aagataataa agaaagtata cgagagatat atacagcggc agttataatt tcattcttta
      361 taacattatt atgttgtgtg attataagtt ttattaatat cgacttgata tataaattgt
      421 ttttatcttg tgttttttta tttgctatag aaacaaatat ttgtagagcc ctatgccttt
      481 cattgaataa acctgaattg gcctctgcag taactaatat tccgaattta tttttattga
      541 tatatgtgtt ttgttattcg tcatttaaca taaacaatgt gatggatata tattgttatg
      601 gctctgccat gatgttcgta tcaattttta ttattttata taaatttaat attaagttgt
      661 tttggccagt tttccaccct tcttacttaa gattttctat catgaaatac tctctactta
      721 gaggtggtgg tggtttcttt atggttcaat tggtttcgat ggggttaata ataagtgata
      781 gatatataat atcatctata tttggtgttg aagatgtcac aaaatatgat ttgatattta
      841 aggtgtcaaa tttttgcata atgttttttt caattgtaaa tatggttctt tggtcgaaat
      901 ttacagaatt ttggatgcaa gggttttata caaaaatcaa ggttatcttt aaagtgtttg
      961 acttatgttt tttattgtta gttatcgtcc taattttgat tttaaaccat atacactgga
     1021 tttttgaatt atggctaaat gtaactaatg aaaattacaa tgaagtttat attacaggga
     1081 ttgtccttta tttggttggg tattattgga tgtcagctta ttcctctttt ttaaatggta
     1141 taagcatcat aaaaaaacaa tttattatcc agggtggagg gcttttgatc aaagctatta
     1201 ctttattaat tgctatgaat atcttgaaag tatttaaggt tgaatatatt attttgctgg
     1261 gtggtgcgtg tctatttatt gttggttttt tattcaggaa gtgtgcgcat gataaaatca
     1321 atagttgtat atagctttcg gatttattat ttcatcaata gatggattac aacaaaaata
     1381 aataaattga ggttgaaagc taactctgtc gaattaggtg acagcgtaat gattaatgga
     1441 atattgcata tagttaacaa gggggaaatt aaaattggta acggtgtcaa gattaattct
     1501 tcattacgtt acaatccaat ttcacatcat ccatgttcat tttacacttc tgaaaatgcg
     1561 gtgattgaga ttggggataa atccgggttg tcgggttgta ccttatattc tgttaaaaaa
     1621 atccatattg gaaaaagagt gttgattggt gcaggcgtaa atatttatga tacagatttt
     1681 cattctttac tcgtggatga gagattaaat ggtgatagta acattaaatc ctctccagtt
     1741 tatatatacg atgattgttt tattggagct aatgttacaa ttttgaaagg tgttacaata
     1801 ggggctggca gtgttgttgc tgcttgctcg gtagtaacga agaatatagg ggaaaatgaa
     1861 ctatgggcag gcaatccagc taggtatatt agagatacta tatatggcga atgataaaat
     1921 taccgttata actataaatt acaataatgc agaaggctta tcaaaaacga ttgcttcagt
     1981 tgaaaagcaa actaataaag aatttgagta tctaattatt gatggtggtt ctgaggataa
     2041 ttcaatagaa catgctaaga aaagtgtaat atgcacaaaa ataatatctg aaaaagataa
     2101 tggaatctat gatgcaatga ataaaggagc ctcacttgct aaaggtgagt atatcatttt
     2161 tacaaactct ggtgatgaat taagaaaaaa tagcattgaa attttgatga atgtattgag
     2221 agagacaaat acattatacg atatatacca cgggattttg gctttcaaaa aaaatgataa
     2281 agttatttat tatagaggtc ggaaaagtga catgttgaac agttcaatga ttgaacatcc
     2341 tactgtgtta atgcgtaaaa gcacatttaa tgaattaaaa ggatttgata ataagtggcg
     2401 atatgtagct gattatgatt taatgttacg ggcatatttt acaaaaagaa gatttttttt
     2461 tattgaggag gtgattgctg attttgatgt atcaggtgta tctagtgtga gtattaaagc
     2521 tcctttggag tctttacggt tacaacggaa atataattta attaatacat ttgaatattt
     2581 tagcaagtta ttttttatca agatgaaaaa ggctgtatca tgcttaaggt aatccttctt
     2641 tcatcaatca acccagaaca aaaaacaggc tactataatg ctgtcaatga ccgtgtaaaa
     2701 tctctaaaaa aagagaaagg aataaaaatt tggtcatatt cgatatataa aagcacgtca
     2761 ttgataagat tctcaataaa aaaaaatgaa aatcattttt ttatagaata tcccaaagca
     2821 tttccttcgc atggtttatt ttcattcata actctctggt ttataagatg tgttatttta
     2881 tttctgtggg ttataataag acctaacatg atacatgtgc attggggata tccgcttgca
     2941 tatgctgctg taattataaa aagattaacc aaaaaaacaa aagtcattgt gactttccat
     3001 ggctctgatg tgcatacaca tcctgccaga tcgaaagaaa ttttcgtaaa aacttgtaat
     3061 ttatgtaaaa gagttgatca tgttacggct gtgagtgaga acttattatt gcaagtaaga
     3121 aataagtttt cagtgtcaga atcgaaatca tctataacat ataatggagt taacctagat
     3181 gaaactaatt gttctttttc atcaagtaat ggattgacgt tttcgttttt tgggaatcta
     3241 aatatgacta aaggtgctga taggataatt ccactttata atgcactttc agggttaatt
     3301 gaagagaact ttacatttta tgttggcggg gaaggacctt gttataagga aataaagcaa
     3361 tatattatcg taaatggaat ctctaatatt aaattgctcg gttttatgcc tcgtgctgat
     3421 gtaaaacgaa taatgcgcaa aagtaattgt gtctttatat taagtcgtaa tgaagggttg
     3481 ggtattagtg caattgaagc attagcttat agtcaattat gtatagcacc tgatataggt
     3541 ggattatctg aagtttttag agagaatcca gagttattaa ttaaaaatga ttttaatcca
     3601 tcagactatg ctaataagtt tattagttgt cacaaagata aattcagagt taatcgtaca
     3661 tatatattta aaaacttttc tatgaaaaac ataattcaga aagaaataac cttatataat
     3721 gaaataatga aataatgaaa caggcacaaa atgaattgta aatttaacat ttgggatatt
     3781 cagttaggtt tgatattaat atcagtacta tcttatgctt ttttaaactt gggtttttta
     3841 ccattaaata tttcccatct aataatttta tttgtgttga taacgctaag tgttaatctg
     3901 ttaattagcc aatcaattaa aataactgga ttatttttaa ctctttttgt gtcctttatt
     3961 ttactttctg cttattcatt attgaaatat tatgatgttc aaaaagtaaa aaatataatt
     4021 aattttttta ttttttttag cgttgctgct atagtgataa ataactgcag tgctgataaa
     4081 ataataaaat tttattataa attaactaaa atttttatat tttttgcttt acttcagtgg
     4141 gttctttatt atctcaacat tggaacacta tatacatatt cttttcttgg tttaaaggaa
     4201 gttaatattt caacaagtgg atatttaatt cggctattct ctatagctag tgagcctgct
     4261 gcattatgtg ggatattgct tccagctata tatctatcta taaatagaat agttaataag
     4321 ggtaaagaag ttactttatc atattcagta attgttttat ttgttatttt aaatacattt
     4381 tcgttggttg gctatatata tataatcatt tgtttaatag tagcgctata tgtaggtaat
     4441 aaaatctcat taagtaaaat ctttattttc acaatttgtt tggcattgct tgtctttatt
     4501 ctttttcaat cggatagtat ccagcagcgt ttaaatgaaa taactagtct agataaaatg
     4561 gctagctcag acaatctttc tgttatagca atttattcaa atttgcaaat agctttgata
     4621 agtctcagtg ataatcttat atttggggga ggaatattta gtcatcctta tacatacgat
     4681 cattatatca gtcaattgta tgctggtgga ggaccacgca tggaattaaa taaggatgat
     4741 gcagcttctc tttacatacg tgcattatca gaaacgggaa ttttaggttt ttgtattttg
     4801 aatgggttga ttatatatct gatgaaacgc tgcgataaat ctaaaatcaa ctatccatat
     4861 aacatcgctt ttacaattgc attcgcattg cttggtatta gagctggtag tgttaattat
     4921 ataatcattt ggttctattt tttttccgct atacggttcg taaatgaagg aaggttaaaa
     4981 tgaaaatgtt atcgtttttg gaatgtatga atagcactta tggagggcca gcattttcac
     5041 ttccttcttt acttcatcat ttagaaaaaa actatcttgt tcaaagtaca gttatttcgc
     5101 ttaaaaagaa gaatgaagtt tatgaaaata atgttgctga atgtttggga ataaacccga
     5161 ttatattaaa tcaggttggc ccaagaaagt taaaattaac acacgagata gcaaaagtaa
     5221 aaaaaataat gcaagattgt gatataattc atacgaacaa cttgtggaat tatttttctt
     5281 atttgcccta taatttatca aaaaacagta aaaagccgaa tgttatttcc gtcagaggca
     5341 gtttatatgc ttggtctctt gaacagtcga gactgttaaa aaaaatctca tggtttcttt
     5401 ttcaaaagaa agcattggat tcagccggtt ttatacatgt tacttgtaac gatgaattaa
     5461 cagctttacg agaattaggt attaaaaata aaatagttgt ttcacaacat ggtgtggatg
     5521 tacctgattt gatagataaa gttccagata gtttttatga taaatataaa ttatcgcata
     5581 ataaaaaata ttttctattt atgtctcgac tacatacaaa aaaagggcta gatatgttgt
     5641 ttaatgtatg gtcgaggatc tctcaccatt atcctgattg ggaattactt gttgctggtc
     5701 ctgattatgg aaactatata ccaaaaataa ctaagttaaa tgcagtaaaa tatatgggaa
     5761 tgctaagtgg ggaagagaaa gaatactgtt tttctatagc tcaatttttt gttctacctt
     5821 catacactga aaattttggc gttgtaattg gtgaggctat ggcaagaacc gtacctgttt
     5881 taacttctac taatacacca tggaaagatg ttaataccaa taatgcaggt gtatgtttcg
     5941 atttatcaac tgaaaattta aaaagagaat tggagttttt tttcaattta tctgattcaa
     6001 ctagaaaaga aatgggaagg aatgcgagag aattgatagt tagaaactat tcatgggaac
     6061 gagttagtta tcctttttac gatgcactta aatcactttg aggtttaata gaatgaaaaa
     6121 agcattaatc acaggcatca ccggtcaaga tggatcttac ttagcagaat tccttcttga
     6181 taaagggtat gaagtgcatg gcattaaacg tcgagcatct tcatttaata cggagcgtgt
     6241 tgatcatatt tatcaagatc gccacaatca aaatccaaat ttctttcttc attacggtga
     6301 tttgacagat acttcgaact tgatacgtct cattaaagag atccaaccag acgaagttta
     6361 taaccttggc gctcaatctc atgtagcggt ttcattcgaa tcacctgaat atactgctga
     6421 cgtagatgca atggggactt tacgtctgct ggaagcgatt cgtatttgtg ggttagagaa
     6481 aaaaacacgc ttctatcagg catctacttc tgaactcttc ggcttagttc aggaaatccc
     6541 gcagcgagaa acaacaccat tctaccctcg ttcgccttat gctgttgcta agatgtatgc
     6601 atactggatt actgtaaact atcgtgaatc ctatggaatg tacgcctgta atggtattct
     6661 cttcaaccat gaatccccgc gtcgtggtga aacattcgta acacgtaaaa tcacgcgtgc
     6721 tattgcaaat atttcgcagg gaatcgaaaa atgtctctat cttggcaata tggattcact
     6781 gcgcgactgg ggacatgcga aagattacgt gcgtatgcag tggatgatgt tgcagcaaga
     6841 tcacccagag gattttgtta ttgcaacagg caaacaaatt tccgtacgtg aatttgttcg
     6901 tatgtcagct aaagaagtcg gtctggagtt agaattctcc ggacaaggtg ttgatgaaat
     6961 agcaactgtt gtgaataaaa catctgactg tgctattggt gttagtattg gggatgtgat
     7021 cgttcgtgtt gatcctcgtt atttccgtcc tgcagaagtt gaaactcttc ttggtgatcc
     7081 agcaaaagcg aaaaaagtat tgggttggga accagagatt acagttgaag agatgtgtgc
     7141 agaaatggtt gccagtgatc tggcgaaagc aaaacagcat gcactactga aaagccatgg
     7201 ttacgatgtt gcagtttctc tggagcggta aggtatgaca aaaaaacgta tctacgttgc
     7261 tggtcaccga ggtatggttg gctctgctat ttgccgtcaa ttatcactgc gtgatgatat
     7321 cgaattagtg gtcaaaacac acaaagaact cgatctaacc gtacagaagg aagttgatgc
     7381 attttttgag caagagaaaa ttgatcagat ttatcttgct gcggctaaag tcggtggcat
     7441 ttatgccaac aatacatttc cggcagaatt catctatcag aatctcatga ttgagagtaa
     7501 tattattcat tcagctcaca aggccggaat tcaaaaatta ctttttttag gctcaagctg
     7561 tatttatcct aaattcgcag agcagccgat gaacgagtct gcgcttttaa cgggcatact
     7621 tgagccaact aatgagccat acgcaattgc taaaattgcg ggcataaaat tatgtgaatc
     7681 ttataaccgt caatatggcc gtgattatcg cagtgtaatg cctactaatc tttatggcat
     7741 aaatgataat ttccaccccg aaaactctca tgttattccg gcactcatgc gtagattcca
     7801 tgaggcaaaa gagagtggtg caccagaagt tattgtctgg ggaacaggaa caccgatgcg
     7861 tgagttttta tatgttgatg atatggctgc cgcatctgtt catgtaatgg aacttgacga
     7921 agcaatttat caacaaaata cacagcctat gttatctcat attaatgttg gtacgggtgt
     7981 agattgttcc atacgtgaaa tggctgaaac aatggcctct gtggtgggtt atcaaggtaa
     8041 aattgttttt gatgttacta aacctgatgg cactccgcgc aaacttatgg acgttacccg
     8101 gctcaaaaac ttgggctggc aatatcaata taacttacat gaaggcttat cattaacata
     8161 taaatggttt attgagaata ttaattcttt tcggggatag ttatgaataa gagattggaa
     8221 cgtgagctat ttaaaacaat agttgagcat actcctctaa tctcgattga tctcataatt
     8281 agaaacgata aaggagaggc gctgctgggg cagcgcctga atcgcccagc acaaaattat
     8341 tggtttgtgc caggagggcg aatttataag gatgagtcat tcgagattgc atttaagcgg
     8401 ataacatttg aagaggtggg cgttcaaatt agtcttaatg atgccttatt tctaggggtg
     8461 tatgaacatt tttacaatga taatttttct gaagcagaat tttctacaca ctatgtagtg
     8521 catggatatg aaatccaact taaacctcag caacttcacc taccaacggt ccagcataat
     8581 tcctacaagt ggtttgatgt agtaacgttg cttaatagca ctacagttca tcaatatacc
     8641 aaaaattatt ttataccaag gtaatagata tgctacttcc cgttgtcatg gccggtggtt
     8701 ctggtaccag attatggcct ctttcacgta cactttatcc gaaacaattt ctgtctttaa
     8761 atagtcgttt aaccatgttg caagagacat tgcggcggct tgacaaggta gaacataaac
     8821 ccgctttggt catttgtaac gaatcacatc gctttatcgt tgctgaacaa ttgcgtaaag
     8881 agggtttaaa gcatagcggt attttgcttg agcctgttgg tcgtaatact gcgcctgctg
     8941 tagcactcgc agcactacag gctatggtaa ctggagatga tcctattctg ttggttcttg
     9001 ctgctgatca tgaaattcag aatgaggata atttcattga tgcagttctt gcagccaaga
     9061 attttgcaga acagggtaag cttgttacgt ttggtattgt tccaacatcc ccagaaacgg
     9121 gctatggtta cattaagtca ggtgaatatc tggatggaaa aggttacaaa gttgctgctt
     9181 ttgttgaaaa accagagctt cacgtagctc agcagtatat atcagatggc ggttatcttt
     9241 ggaatagtgg gatgtttatg ttcagagcat ctgtatttat cgatgaactg aaaaaattcc
     9301 gaccagatat tttagccagt tgccaacgct ccttgtcctc ttcgatacaa gatttagatt
     9361 ttatccgtct ggataatgct tcattttctt gctgtcctga agagtctatt gactatgcag
     9421 ttatggaaaa aacagccgaa gctgtcgtcg ttccattaaa tgcacaatgg agtgatgttg
     9481 ggtcatggtc tgcattgtgg gaaataagtt caaaagatca aagcggtaat gccattcgtg
     9541 gtgatgtatt ggttgaagat gctacagata gttatcttta ttcgcagcat agacttattg
     9601 gtgccgtggg cataaaggat ttggttgttg ttgaaacgaa agatgccgta ttagttgctc
     9661 ataaagataa agttcagcaa gttaaaaaca tcgtcgctca acttaaaaag aataatcgca
     9721 cagaatattt acagcatcgg gaaatatttc ggccttgggg cagtcatgat actatagctg
     9781 aagggccgcg ctttcaagtg aaacatgtga ttgtattacc tggccacatt accgctaagc
     9841 aaattcatta ccatcgtact gagcattgga ttgttgtctc ggggacagct aaagtccatc
     9901 ttgaagataa gacttacctt gtttctgaaa atgaatcaac atatatacct gttggtgttc
     9961 cacatgccat tgaaaatcct ggcaagatcc cgcttgaaat aatcgaggtc agatcaggag
    10021 tctacctgga agaggatgat gttataagag tttcttcttc tggagtagga tactaatgcg
    10081 aatttctatt atcacagcta cttataatag tgaaaaaact ctccttgata cattactttc
    10141 tctagaaaaa caaacgtatc cagatatcga atatatagtt gtagatggag catcgaacga
    10201 taatacaatt aagctgatta agagtaattg tacaaaagtt tcaaaaatca tttgcgaacc
    10261 cgataagggc atttatgatg ctctgaataa aggtattcaa gcagcttcgg gtgatgttat
    10321 tggtttttta cattctgatg atttattagc ttatgatgat gctattgcag atatagcaaa
    10381 aacatttgaa attacagggt gtgatgctat ttatggcgat ttggagtatg ttgcccaaaa
    10441 tgatacgact aaacgtatta gattatggaa aagtggttca tttagtcgtt tcaagatgaa
    10501 agtgggttgg atgccgccac acccatcatt ttatatgaaa cgcaaatgtt ataggcagtt
    10561 tggttgtttt tctttagatt atcgtatatc tgctgattat gactcattat tacgatatat
    10621 tttaaagcaa cgtatttcaa tagaatattt accaaaagtc ttagtgaaga tgcgtgttgg
    10681 tggaattagc aatcgttcag tctcttcaat ggtaaagaag tcaatggaag acatccgtgt
    10741 tatgaaccag aatggtatta tctggccaat agctttagtg tataaaaata tatccaaact
    10801 tcctcaattt attaaaaagt aatcatcatg ttaaatgtta aaaaaatcat taacgatagt
    10861 aatattgcgt tcggtactag tggtgctcgc ggtttagtga tcgatttttc ccatgatgtt
    10921 tgtgctgcgt tcactcatgc gtttctttct gttattgatg acaaatacaa ttttaataaa
    10981 gttgccttag caattgataa ccgcccaagc agttacgaaa tcgctcaggc atgcgccttc
    11041 gctatcaaac aacatgggtt cactgtcgaa tatcatggtg taattccgac tcccgcatta
    11101 gctcattatt cgatgcagaa aaacattccc tgcataatgg tcacagggag tcatatccct
    11161 tttgatcgta atggcttaaa attctacaga cctgatggtg aaatcactaa agaggatgag
    11221 ctcgcaatta taaatagtga gtatacattt ccgcctgtag gtgtattacc tcatcttgaa
    11281 acaagcactc aaggtgcaga ctactacctt gagcgttatg tttctctttt taatcccgag
    11341 attttaaagg ggaaaagaat aggggtatat gaacattcta gtgcgggacg cgatttatat
    11401 gctcctcttt ttaatcaaat gggtgcagag gtcatttccc tcggcaggag tgatgagttc
    11461 gttccgattg atacggaagc agtaagcgat gaagatcgta tacttgcaag agagtggtct
    11521 aaaaaatata atcttgatgc tattttctca acagatggcg atggtgatcg tcctttagtt
    11581 gctgatgaaa atggtgaatg gttaagaggc gatattctgg gactacttac tgctattgaa
    11641 cttaatatca aggcgttggc tattccagtg agttgtaata cagcaattga agagtctaat
    11701 aaatttgcaa gtatacaacg aacgaaaata ggttctcctt atgtaattgc agcatttgca
    11761 gatcttgcta agcaatttga ttcagtcgca ggttttgaag ctaatggtgg ttttctcctt
    11821 gcctccgatt tacaaattaa tggcaaggaa ttaaaatcgt tgcctacacg agatgctgtg
    11881 ttaccagcat taatgctctt aatagcttct cgcaatagta ctatttctca actgattaat
    11941 aatcttcctc agcgattcac ttggtcagat agagttaaaa acttcccttc agattcaagt
    12001 caacaaatta taaaaaatgc catatcgtca cccaataatt tctttaatag tttaggttat
    12061 gaatcattat cctgttccgc tattgatgaa acggatggtg caagatttac tttaaataat
    12121 ggtgatatta tacatctccg tccttccggt aatgctccag aactccgttg ttatgctgag
    12181 gccagtgatg aaaatcaggc taagcaatat gttacgaatg tgctgggaaa tattacctct
    12241 ttgatttctt gatgttatag gtttatctac gcttatatgt gtgcgtaggt ttgattacac
    12301 gtagatgccg gtatacagaa ttgaagaacg gtatttgttg cattaatgaa attcagcact
    12361 acacacattc gtgcaacttg agataacatc tcaatcatat tcaagtcgcg catacatcgc
    12421 ggtgaacacc ccctgacagg agtaaacaat gtcaaagcaa cagatcggcg tcgtcggtat
    12481 ggcagtgatg g
//
