LOCUS       KJ778795               15394 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain 3461-54 serotype OX9:H? O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778795
VERSION     KJ778795.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15394)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15394)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="3461-54"
                     /serotype="OX9:H?"
                     /db_xref="taxon:562"
     misc_feature    1..15394
                     /note="O-antigen gene cluster"
     gene            125..1201
                     /gene="rmlB"
     CDS             125..1201
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG62717.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEQSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRDQDGSYQCERLGLVK"
     gene            1198..2070
                     /gene="rmlA"
     CDS             1198..2070
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase 2"
                     /protein_id="AIG62716.1"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIQDILIITTPEDQQGFIRLLGDGSQFGINLTYAIQPTPDGLAQAFIIGENFIGNDTV
                     CLVLGDNIFFGQGFTPKLRDAAERTSGATVFGYQVMDPERFGVVEFDRNFKALSIEEK
                     PANPKSNWAVTGLYFYDNNVTDIAKSIKPSKRGELEITAVNEVYLKNNNLTVELLGRG
                     FAWLDTGTHDSLIEAGSFVETVQKRQGLMVACPEEIAWRNGWLNHDELNDKGEFLKKN
                     HYGQYLLRLIKSHE"
     gene            2063..2470
                     /gene="fdtA"
     CDS             2063..2470
                     /gene="fdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-4-oxo-6-deoxy-alpha-D-glucose-3,
                     4-oxoisomerase"
                     /protein_id="AIG62715.1"
                     /translation="MNNKAKLIQLDKKSDHRGALIVVEQNKDIPIEIKRVYYMFDTGH
                     DVRRGYHAHKTLTQIAIPVKGSCKFLLDDGKTIEHVTLDSPSVGLIIEPLVWHEMYDY
                     SPDCILMVLADDLYDESDYIRDYAEFIREITNE"
     gene            2463..2996
                     /gene="wbtB"
     CDS             2463..2996
                     /gene="wbtB"
                     /codon_start=1
                     /transl_table=11
                     /product="butyryltransferase"
                     /protein_id="AIG62714.1"
                     /translation="MSKLLLSTKNTELIPVEISDAEFIYSLRIDSRLNKHISSINGTI
                     IDQAKWIEDYKKREKNNEEFYFIIKRTDNDKKIGTIRLYDITKDNGFCWGSWILNEDK
                     MKSSAIESAYLIYKFGFFHKKYKFSYFQVDKKNKEVIAFHKKTGAAIIKEDEVNYHFE
                     FHMENFIKLKEKYSKYI"
     gene            3009..4112
                     /gene="fdtB"
     CDS             3009..4112
                     /gene="fdtB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
                     transaminase"
                     /protein_id="AIG62713.1"
                     /translation="MIDFLNLKRINAQYEMELKDACSRVIDSGWYIMGTELKEFENEF
                     ASWCGVKHTIGVANGLDALILVLRAWKEMGKLNDGDEVIVPANTYIASVLAITENNLV
                     PVLTEPDPLTFNLSASGILKVITGKTKAILPVHLYGQISPMAEIMEIASEFNLLVLED
                     CAQAHGSSINGRKAGCWGHAAGFSFYPGKNLGALGDAGAITTNDDQLADVLRAIRNYG
                     SHEKYKNKYQGLNSRLDEIQAAMLRVKLLHLDRENGLRRKIAHHYLENIKNNNIQLPV
                     VLTEEGHVWHLFVIKTEHRNKLQQHLKNCNIHTLIHYPLPPHKQKAYASYNSISLPIS
                     ESIHNQVISLPMDPNMTEEDILQVIKAVNIFKP"
     gene            4109..5362
                     /gene="wzx"
     CDS             4109..5362
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="AIG62712.1"
                     /translation="MKRLLKVSALSGMLTLLKMLMGFIIAKVIAVYTGPAGMAMLGQI
                     QNIINSLNGIVNSPVSNGVVRFTAENNKSGFYKCSPWWRASLEWLIILCVITIPLGFL
                     FSDYLSTWLFETKEFGWLIKLTVIFLPLTAFGTLISSVINGLENFRRYIILIMVSVLI
                     SSCLMIVMIIYAGLKGALIAAVFQSSMIGAVMLLTNIKQPWFSLTYWWGNTNSKARKD
                     IAGYILMAVTSAITAPLSLILVRNIIIYHSGWEQAGQWQAVWKISEVYLSIITISLST
                     YFLPKLASKADVGGIIQEINKTALIVIPLAIIMAVVVYAGRDIAIYILFTEQFRAARE
                     LFAIQLLGDIVKIISWLYAYPMLSRGATKWYVSTEILFSASFVILSWFLVGEYGNAHG
                     ANVAYLINYTFYFLFIIVNFKKIIK"
     CDS             5388..6617
                     /codon_start=1
                     /transl_table=11
                     /gene="orf7"
                     /product="glycosyltransferase"
                     /protein_id="AIG62711.1"
                     /translation="MQKIIIYHPSKYLGGTEILFSRVIQLLLSVKLLNIHVVDYPDGI
                     LMGKFHNAKVKYHDVINYNTKDVLEGALIISSARNIYRVFYECVKNDVREIKPMFWLL
                     HPSELYAGYCLGSTFLKKISYTVLKRYIAMLPALKFYINVIKELDINKNLWLMDDTSR
                     RESEWMLNYQFRDDAILPLITNLDCQSISYPVTTQNIKILVISRLDDFKVYGVEKLIT
                     DIIFNKENNNITIEIIGDGEAKNKLIERYNNELNIVFHGYVENAELNRMIENNEYSIL
                     FAMGTSALEGCSRSIPTVLLPCADKSIKGRNNVYKYIHMNDDFSLGEYIDTPFESTGY
                     ITFDDVISTYSKEKYYLREKTKKFFLEKYEKDKTNNDFLNALGQIKSLKITMMKTSLI
                     FEKYTEIIIKQRLKKGF"
     gene            6633..7976
                     /gene="wzy"
     CDS             6633..7976
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="AIG62710.1"
                     /translation="MPIAVYFIEFAYVMIVLLFILYYNSRNMPFIEGLFLILIICSPY
                     SISIIPFRQDMYEIYGIYSNNGILNIVNFYKIAGLSILDMMALLIIVININTVMKMPS
                     FFIMFYFLVGCFSIIGYSISSYLFWSPELDNIFNILSVFKSIIYIISVYCAIDRICKH
                     IGFDKFIKFLANLILCYCIINALILCFLPDWYTWVKYSFNYLFLDQTDQFLVFLYCLL
                     AVWGPLSGIHRYKIYALILCIMLLLSGGKGGLYSLVILAMAWLYKNSNINSAILSICF
                     FLIMILSWMLSYYIGFNVFDLSIYTRYFQVNQLFLNYMNNILTIFFGIGPSKAYFFYS
                     EPAILDPGAYTSSELNSSFKIGFQMPYLTWIKNFGLSGLILLYLSLNYVVKTARHSKS
                     NEAYFSGIVYALGLYFLLVGFMDFPSFGLKTIIPIGLYIYIVKMNATNIHCEGRK"
     CDS             7973..9010
                     /codon_start=1
                     /transl_table=11
                     /gene="orf9"
                     /product="glycosyltransferase"
                     /protein_id="AIG62709.1"
                     /translation="MIIIDGIIFSLQKAGGISVYFYELLKYIQKANKEYLHLLYNNQN
                     AYANGNGFIINSKTRLHISIERFRRVPIIGDETDIFHSSYYRLPERSFSGKIITTVHD
                     FTEEMYPRGIVSKIHHIQKRNAILGSDGIICISKNTMMDMHRLIPESIDIPTTVIYNG
                     VGDFKSKECLGEYEPYVIFVGARNGYKNFNMCISALKKINDIRLVVVGGGGFTDNELK
                     LLNTLIPGRYSHAGFITEIELNKLYQNSLALIYPSSYEGFGIPVIEAMKSGCPVIASN
                     SSSVQEISNNAALLIDSICDKKIADAIQSLRNPAFRQEKIKLGIMNATNYSWDKMANE
                     TLSFYNTIRGL"
     gene            9010..10128
                     /gene="gmd"
     CDS             9010..10128
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="AIG62708.1"
                     /translation="MKVALITGITGQDGSYLAEFLLNKGYEVHGIKRRTSFFNTTRID
                     HIYQDPHVNNPKLHLHYGDLTDTSNLTRILSEVRPDEVYNLGAMSHVAVSFESPEYTA
                     DVDAIGTLRLLEAIRFLGLEKKSRFYQASTSELYGLVQDVPQKETTPFYPRSPYAVAK
                     LYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAMANISQGIEKCLYLG
                     NMDSLRDWGHAKDYVRMQWMMLQQDQPEDFVIATGKQISVREFVSMSAKEVGLELEFT
                     GKGVDEIATVVNKTSDCAIGVNVGDVIVRVDPRYFRPAEVETLLGDPEKAKKVLGWEP
                     EITVEEMCAEMVASDLAKAKQYALLKSHGYDVSVSLER"
     gene            10132..11097
                     /gene="fcl"
     CDS             10132..11097
                     /gene="fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="AIG62707.1"
                     /translation="MIKKRVYVAGHQGMVGSAIYRQLSVREDIELVVKTHKELDLTVQ
                     KDVDAFFEQEKIDQVYLAAAKVGGIYANNTFPAEFIYQNLMIESNIIHAAHKAGVKKL
                     LFLGSSCIYPKFAEQPMNESALLTGILEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGINDNFHHENSHVIPALMRRFHEAKESGAPEVIVWGTGTPMREFLYVDDMA
                     AASVHVMELDEEIYQKNTQPMLSHINVGTGVDCSIREMAETMASVVGYQGKIVFDATK
                     PDGTPRKLMDVTRLKNLGWQYRYNLHEGLSLTYKWFIENINSFRG"
     gene            11100..11561
                     /gene="gmm"
     CDS             11100..11561
                     /gene="gmm"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="AIG62706.1"
                     /translation="MNKRLGCDLFKTIVEHTPLISIDLIIRNDKGEALLGQRLNRPAQ
                     NCWFVPGGRIYKDESFEVAFKRITFEELGVQISLNDALFLGVYEHFYDDNFSEAEFST
                     HYVVLGYEIQIKPQQLHLPTVQHNSYKWFDVVTLLNSTIVHQYTKNYFIPR"
     gene            11567..12973
                     /gene="manC"
     CDS             11567..12973
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AIG62705.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLNSRLTMLQETLRRL
                     DKVEHKPALVICNESHRFIVAEQLRKEGLKHSGILLEPVGRNTAPAVALSALQAMVTG
                     DDPLLLVLAADHEIQDEDNFIAAVLAAKNLAEQGKLVTFGIVPTSPETGYGYIKSGES
                     LDGQGYKVAAFVEKPDLHVAQRYISNGGYLWNSGMFMFRASVFIDELKKFRPDILASC
                     QSSLSSSIQDLDFIRLDNASFSCCPEESIDYAVMEKTAEAVVVPLNAQWSDVGSWSAL
                     WEISSKDQSGNAIRGDVLVEDATNSYLYSQHRLIGAVGVKDLVVVETKDAVLVAHKDK
                     VQQVKNIVAQLKKNNRTEYLQHREIFRPWGSHDTIAEGPRFQVKHVIVLPGHITAKQI
                     HYHRTEHWIVVSGTAKVHLEDKTYLVSENESTYIPVGVPHAIENPGKIPLEIIEVRSG
                     VYLEEDDVIRVSSSGVGY"
     CDS             12973..13788
                     /codon_start=1
                     /transl_table=11
                     /gene="orf14"
                     /product="glycosyltransferase"
                     /protein_id="AIG62704.1"
                     /translation="MRISIITATYNSEKTLLDTLDAHMKVSIITVTYNSEKTIKDTLD
                     SVAVQTYNNIEYIIVDGASSDATLHIINNSSASVAKCISERDKGIYDALNKGVHLATG
                     DIIGFIHSDDILARPDIIETIVIEFQKTKADIVYGDLVFVDRSDVNNIVRYWHSGSFR
                     KSKISLGWAPPHPSFYMKRSLYKEDGFFDLTFKIAADYDQMIRVLKRERIKVIYLPQV
                     FVKMRLGGESTKLDNTVASTKEIISIMKKHHINWYIAIVTRKFSKLTQLFRKS"
     gene            13819..15189
                     /gene="manB"
     CDS             13819..15189
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="AIG62703.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVEFIKVHNTPDGNFPNGIP
                     NPLLPECRDGTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
BASE COUNT     4852 a   2500 c   3213 g   4829 t
ORIGIN
        1 aggcatgcat gcagtgctct ggtagctgta aagccagggg cggtagcgtg cattaatacc
       61 tctattaatc aaactaagag ccgctaattt cacagcatgc tctgaagtaa tatggaataa
      121 aaaagtgaag atacttgtta ctggtggcgc aggatttatt ggttctgctg tagttcgtca
      181 cattataaat aatacgcagg atagtgttgt taatgtcgat aaattaacgt acgccggaaa
      241 cctggaatca cttgctgatg tttctgattc tgaacgctat gtttttgaac atgcggatat
      301 ttgcgatgct gctgcaatgg cgcggatttt tgctcagcat cagccggatg cagtgatgca
      361 cctggctgct gaaagccatg tggatcgttc aattacaggc cctgctgcat ttattgaaac
      421 caatattgtt ggtacttatg tccttttgga agcagctcgc aattactggt ctgctcttga
      481 tggcgacaag aaaaatagct tccgttttca tcatatttct actgacgaag tctatggcga
      541 tctgcctcat cctgacgaag taaataataa agaagaatta cccctcttta ctgagacgac
      601 agcttacgcg ccaagcagcc catattctgc ttctaaagca tccagcgatc atttagtccg
      661 cgcgtggaaa cgtacctatg gtttaccgac cattgtgact aactgttcga ataactacgg
      721 cccttatcac tttccggaaa aattgattcc gctggtaatt cttaatgctc tggaaggtaa
      781 ggcattacct atttatggca aaggggatca aattcgcgat tggctgtatg ttgaagatca
      841 tgcgcgtgcg ttatataccg ttgtaaccga aggtaaagcg ggtgaaactt ataacattgg
      901 cggacacaac gaaaagaaaa acatcgatgt tgtgctgact atttgtgatt tgttggatga
      961 gattgtaccg aaagagcaat cttatcgtga gcaaattact tatgttgccg atcgtccggg
     1021 acacgatcgc cgttatgcga ttgatgctga gaagattagc cgcgaattgg gctggaaacc
     1081 gcaggaaacg tttgagagcg ggattcggaa gacaattaac tggtatttaa ataataatga
     1141 ttggtgccga cgagatcaag atggctctta tcaatgtgag cgtttggggt tagttaaatg
     1201 aaaggaatta ttttagccgg tggttctgga acaagacttt atccaattac aatgggggtt
     1261 tcaaagcagc tattacccat ttatgataaa ccgatgattt attatccatt atcggttttg
     1321 atgttagcag gtatacaaga tattcttatt attacaacgc cagaagatca gcaggggttc
     1381 ataagattac ttggggatgg aagccaattt ggtattaatc tgacatatgc aattcagcca
     1441 actccagatg gtttagccca agcatttatt attggtgaaa actttattgg aaatgacaca
     1501 gtatgccttg tgcttggaga taacatcttt tttggacaag gttttacacc caaacttcgg
     1561 gatgcagcag aacgaacatc aggtgccacc gtgtttggat atcaagtaat ggatcctgag
     1621 agatttggag tggtggagtt tgacagaaat tttaaagcgt taagtattga agaaaaacct
     1681 gctaatccaa aatcaaattg ggctgtcacg ggattatatt tttatgacaa taacgtaacc
     1741 gatattgcaa agagtattaa accatctaaa cgcggtgaat tagaaattac tgcagttaat
     1801 gaagtttatc taaaaaataa taatttaact gttgaattac taggacgtgg ttttgcatgg
     1861 ctggatacag gcacccatga cagtttgatt gaagctggca gttttgtaga gacagttcaa
     1921 aaaaggcaag ggttgatggt agcatgtccg gaagagatag catggcgaaa tggctggtta
     1981 aaccatgatg aattaaatga taaaggagag tttttgaaaa aaaatcacta cggacaatat
     2041 ttactcaggt taatcaaatc acatgaataa taaagcaaag cttatacagc tagataagaa
     2101 aagtgaccac cgaggcgctc ttattgttgt cgagcaaaat aaagatattc caattgaaat
     2161 taaacgagtt tactacatgt ttgatacagg acatgatgta aggcggggat atcatgcaca
     2221 taaaacactt acacaaatag caatacctgt taaaggatct tgcaagtttt tgcttgatga
     2281 cggtaagacg atagagcatg ttacattaga ttcaccttca gtcggattaa ttatagagcc
     2341 gttggtttgg catgaaatgt acgattattc accagattgt attttaatgg tgttggctga
     2401 tgacttatac gatgaaagtg attatattcg tgattatgct gaatttataa gggagataac
     2461 taatgagtaa attgttgctt tccacaaaaa acacagaatt aattccagtt gaaataagtg
     2521 atgcagaatt tatttattca cttcgcattg attctcgtct caataagcat atttcttcaa
     2581 ttaatgggac gattattgat caggctaaat ggattgagga ttataagaaa agagaaaaaa
     2641 ataatgagga gttttatttt atcataaaga gaactgataa tgataaaaaa attggtacaa
     2701 taagacttta tgacattacg aaagataatg gtttctgttg ggggagttgg attttaaatg
     2761 aagataaaat gaaaagttct gcaatagaaa gcgcgtatct gatatacaaa tttggttttt
     2821 ttcataaaaa atataaattt tcctattttc aagttgataa aaaaaataaa gaagtaatag
     2881 cgtttcataa aaaaacagga gctgcgataa tcaaagagga tgaagtgaat tatcactttg
     2941 aattccatat ggaaaacttt attaaattaa aagaaaagta tagtaaatat atttaagagg
     3001 gacagctaat gattgacttt ttaaatttga aaagaataaa cgcccaatat gaaatggaat
     3061 taaaagatgc ctgctccaga gttattgatt ctggttggta tataatgggg actgaattaa
     3121 aagaatttga gaacgaattt gcaagttggt gtggagtaaa gcacactatt ggtgttgcta
     3181 acggtttaga tgcacttata cttgttcttc gagcctggaa ggaaatgggg aaattaaatg
     3241 atggagatga agttattgta cctgccaata catatattgc gtcagtatta gcaataactg
     3301 aaaataatct ggtgcctgta ttaactgagc cagatccttt aacatttaac ttaagtgcaa
     3361 gcgggattct taaagtaatc acaggtaaaa caaaagcgat tttgccagta cacttgtatg
     3421 gacaaatatc tccaatggca gaaataatgg agattgcctc agagtttaat ttattagttt
     3481 tagaggattg cgctcaagct catggctcct caatcaatgg acgcaaagct ggctgttggg
     3541 gtcatgcagc aggattcagc ttctatccgg gaaaaaatct tggcgcttta ggtgatgctg
     3601 gtgcgataac aacaaatgat gatcaacttg cagacgtttt gagagctatt agaaattatg
     3661 gctctcatga aaaatataaa aataaatatc aaggtctaaa tagtcgtttg gatgaaatcc
     3721 aagcggcaat gctgagagtt aaattattac atttggatag agagaatgga ctcagaagaa
     3781 aaattgcaca tcattacctg gagaatataa aaaataataa tatacaatta cctgttgtat
     3841 taacagaaga aggtcatgta tggcatttgt ttgtaataaa gacagaacat aggaataaat
     3901 tacagcagca cttaaaaaat tgtaatatac atactcttat tcattaccca ctaccacctc
     3961 ataaacaaaa ggcatacgct tcctataata gtataagttt gccgatttct gaatcaatcc
     4021 ataaccaagt catttcactt cccatggatc caaacatgac tgaagaggat attctgcagg
     4081 ttattaaagc ggttaacatt tttaaaccat gaaacggtta ttaaaagtca gtgcgctaag
     4141 tgggatgtta acattactta aaatgttaat ggggtttata attgcgaaag taattgcggt
     4201 ttataccgga ccagctggaa tggcaatgct tggtcaaatc caaaatatta tcaacagttt
     4261 gaatggtatt gttaattcac cagtcagtaa tggcgttgtg aggtttactg ctgaaaacaa
     4321 taaatcagga ttttataaat gctctccgtg gtggagagct tcattagaat ggctaattat
     4381 actctgcgtt ataactattc ctttaggatt tttattttca gattatcttt caacatggct
     4441 gtttgaaaca aaagaatttg gttggcttat aaagctaacg gttatatttc tccctttaac
     4501 agcattcggt acgctaatat catcagtgat taacggtttg gagaactttc gccgatacat
     4561 tattttaata atggtgtctg tcctcatatc cagttgtctg atgattgtaa tgatcatata
     4621 tgcagggtta aaaggagcat taattgcagc tgtattccag tcatcaatga ttggtgctgt
     4681 aatgcttctt actaacatca aacaaccatg gttttcatta acctattggt ggggaaatac
     4741 aaacagtaaa gcaagaaaag atatagctgg ttatatatta atggcagtga catcagctat
     4801 taccgctcca ttatcactga ttttagtacg taacattatt atctatcatt caggctggga
     4861 acaagctggt cagtggcagg cagtttggaa aatatcagag gtttatttaa gtataataac
     4921 aatttcgtta agtacttatt ttctaccaaa acttgcatca aaagctgatg tagggggtat
     4981 tattcaggaa ataaacaaga ctgctctaat agttatccct ttagccatta ttatggccgt
     5041 agttgtgtat gctggtaggg atattgcaat ttatatttta tttactgagc aatttagagc
     5101 ggctagagaa ctttttgcga ttcagcttct tggtgatatt gttaaaataa taagttggct
     5161 ttatgcctat ccaatgttgt ctcgtggtgc aacaaaatgg tatgtgtcta ctgaaatatt
     5221 attttcagct tcttttgtta ttttatcatg gttcttagta ggtgagtatg gaaatgctca
     5281 tggggcaaat gtcgcatacc tgataaatta cactttctat tttttattta taattgttaa
     5341 ctttaaaaaa atcattaaat agtatagata taaatccagg agaacttatg caaaaaataa
     5401 taatatatca tcctagtaaa tatttagggg gcacagagat tcttttttca cgggtcatcc
     5461 aattgttatt atccgtaaaa cttttaaata ttcatgttgt tgattatcct gatggcattt
     5521 taatgggtaa gttccacaac gcaaaagtaa aatatcatga tgttatcaat tataatacaa
     5581 aagatgttct ggaaggagca ttaattattt ctagcgctag gaatatatac agggtattct
     5641 atgaatgtgt taagaatgat gttagagaaa taaaacctat gttttggctt ttgcatccat
     5701 cagaattata tgctggttat tgtttaggta gtacttttct aaaaaaaata agttatacag
     5761 tacttaaaag atatatagct atgctaccag ctttgaagtt ttatataaat gtgattaaag
     5821 aattagatat taacaaaaat ctttggttaa tggatgatac aagcagacgt gaaagtgagt
     5881 ggatgctaaa ttaccaattt cgagatgatg caatacttcc gctcattaca aatctcgatt
     5941 gccagtccat atcatatcct gttacaactc aaaatattaa aatattggtg ataagtcgtc
     6001 tggatgactt caaggtatat ggggttgaaa aattaataac agatattatc tttaataaag
     6061 aaaataataa cattactata gaaattatag gtgatggtga agctaaaaat aaattgattg
     6121 aacgttataa taatgagctc aatatagttt ttcatggcta tgtagagaat gcagaactta
     6181 atcgcatgat tgaaaataat gaatattcaa ttttatttgc tatgggaact tctgctttag
     6241 aaggatgttc aagaagtata ccaacagttt tattaccttg tgcggacaaa tctattaagg
     6301 gcagaaacaa cgtgtataaa tatattcata tgaatgacga tttctcgttg ggtgaatata
     6361 ttgatactcc tttcgaaagt actggttata taacattcga tgatgttatt tcaacttaca
     6421 gcaaagagaa atattattta cgggaaaaaa caaaaaaatt ttttttagaa aagtatgaaa
     6481 aagataagac caataatgac tttttgaatg cattaggaca aattaaatca ttaaaaataa
     6541 ccatgatgaa aacttcgctt atctttgaaa aatatacgga aatcatcata aagcaaagat
     6601 taaaaaaagg gttttaaatt aggaataaaa aaatgcctat tgctgtatat tttattgaat
     6661 ttgcttatgt aatgatagtg ttattgttta ttctttacta taatagcaga aatatgccgt
     6721 ttatcgaagg tctgtttttg atattgatca tatgctcacc ttattcgatc tctataattc
     6781 cattcagaca agacatgtat gaaatttatg gaatttatag taataatggc attttgaata
     6841 ttgtcaattt ttataagatc gctggtttat ctatacttga tatgatggca ttgttaatta
     6901 tagtgattaa tattaatact gtcatgaaaa tgccttcatt ttttataatg ttctattttt
     6961 tagtagggtg tttttcaata atcggttatt ctatctcatc ttatctattc tggtcaccag
     7021 agcttgataa tatttttaat attctttctg tcttcaaatc aataatatat ataattagtg
     7081 tttattgtgc tattgatcga atttgtaaac atattggttt tgacaagttt attaaatttc
     7141 ttgcgaattt aatattgtgc tactgtatta tcaatgcatt aatactttgc ttccttcctg
     7201 attggtatac atgggtcaag tattcattca attatttatt tctcgatcaa acagatcagt
     7261 ttctggtttt tctttattgt ttgctggcag tttgggggcc attgagtggt attcatcgat
     7321 ataaaatata tgctttgatt ttatgtatta tgcttttatt aagtggagga aagggggggc
     7381 tttattcatt agtaattttg gcaatggcat ggttatataa aaattcaaat attaacagcg
     7441 ctatcctatc aatatgcttc tttcttataa tgatattatc atggatgctt tcttattata
     7501 tagggtttaa cgtattcgat ttgtctatct atacacgtta cttccaagtt aaccagcttt
     7561 ttttaaatta catgaataat atccttacta ttttctttgg cattgggccc tcaaaggcat
     7621 attttttcta tagtgaacct gcaatattag atcctggagc atatacatct agtgagttga
     7681 attctagttt taaaattgga tttcaaatgc catatttaac atggataaaa aactttggtc
     7741 tatcaggatt gatcctttta tatttatcac tcaattatgt tgtaaaaaca gctaggcatt
     7801 caaaatcgaa tgaagcatat ttttctggga ttgtttatgc gctagggtta tattttttat
     7861 tggtcggctt catggacttt ccatcatttg gtttaaagac tataattcca attggtttat
     7921 atatttatat tgtcaaaatg aatgctacaa atatccattg tgagggcagg aaatgattat
     7981 tatcgatgga ataatttttt ctttacaaaa agctggtgga atatccgtat atttttatga
     8041 gttgctgaaa tatatccaga aagccaataa agagtatctg catctgctat ataataatca
     8101 aaatgcctat gcaaatggaa atggttttat aattaatagt aagactagat tacatatttc
     8161 aatcgaaaga tttagaaggg ttccaattat tggtgatgaa acagatattt ttcattcaag
     8221 ttactacaga ttaccagaga gaagtttttc tggaaaaata ataactacgg ttcatgattt
     8281 tacagaagag atgtatccaa gaggcattgt ttctaagatt catcatattc aaaaaagaaa
     8341 tgcgatatta ggcagtgatg gaataatatg tatatccaaa aacacaatga tggatatgca
     8401 cagactaatt cctgaatcta tcgatattcc gacgacagta atttataacg gtgttgggga
     8461 ttttaaaagc aaggagtgct tgggggaata tgaaccatat gtgatttttg tgggggctag
     8521 gaatggatat aaaaacttta atatgtgcat atctgcgctt aaaaaaataa atgatattcg
     8581 gttagtagtt gttggtggag gtggatttac ggataatgaa ttaaaattgc tgaatactct
     8641 tattcccgga cgttactcac atgcaggttt tataactgaa attgaactta ataaacttta
     8701 tcagaattcg ttggcattga tatatccttc gtcatatgaa gggtttggaa ttccagttat
     8761 tgaagcaatg aaatcaggtt gtccagttat tgcatctaat tcttcttcag ttcaagaaat
     8821 atctaataac gctgctttat taattgatag tatttgtgac aaaaaaatag cagatgctat
     8881 tcaaagctta aggaatccag cttttaggca agagaaaata aaattaggaa tcatgaatgc
     8941 cacgaattac tcctgggata agatggcaaa tgaaacattg agtttttaca atactatacg
     9001 aggtttataa tgaaagtagc attgattaca ggtattactg gacaagatgg ctcatatctg
     9061 gctgaatttt tattaaataa aggttatgaa gtgcacggta tcaaacgccg tacatcattt
     9121 tttaatacca cacgaatcga ccatatatat caagatcccc atgtaaataa tccaaaattg
     9181 catcttcact atggtgattt aactgatacg tctaatttaa cacgtatttt atcagaggtg
     9241 cgtccagatg aagtatacaa cctaggagca atgagtcatg tggctgtatc gtttgaatcg
     9301 ccagagtata cagctgatgt ggatgctata ggaactttgc gcttattaga ggctattcgt
     9361 tttttgggat tggaaaagaa gagtcgtttt tatcaggcgt caacatctga attgtatggg
     9421 ttggtccagg atgttccaca aaaagagaca acgcctttct atcctcgctc tccttatgca
     9481 gtagccaaac tatatgctta ttggatcact gtcaactatc gtgagtccta tggaatgtat
     9541 gcatgtaacg gtattttgtt caaccatgaa tccccacgtc gtggggaaac gtttgtaaca
     9601 cgtaagatca cacgtgcaat ggctaatatt tctcagggaa tagagaaatg cctctatctc
     9661 ggaaatatgg attcactgcg tgattgggga catgctaaag attatgtgcg tatgcaatgg
     9721 atgatgcttc agcaagatca accagaagat tttgtgattg ccacgggcaa acaaatttct
     9781 gtacgtgaat ttgtcagtat gtcagctaaa gaagttggtc tggaattaga attcactggt
     9841 aaaggtgtag atgaaatagc aactgttgtt aataaaacat ctgactgtgc tattggtgta
     9901 aatgtaggag acgtaatcgt tcgcgttgat cctcgttatt tccgtcctgc tgaagttgaa
     9961 actcttcttg gtgatccaga aaaagcgaaa aaagtattag gttgggaacc agagattaca
    10021 gtcgaagaaa tgtgtgcaga aatggttgcc agtgatctgg cgaaagcaaa acagtatgca
    10081 ctacttaaaa gccatggtta tgatgtttcg gtttctctgg agcgttaata catgataaag
    10141 aaaagagttt atgttgctgg gcatcagggc atggtcggct ctgctattta ccgacaatta
    10201 tcggttcgtg aagatatcga attagtggtt aaaacacaca aagaactcga tctaactgtc
    10261 cagaaggatg ttgatgcatt ttttgagcaa gagaaaattg atcaggttta tcttgctgcg
    10321 gctaaagttg gtggcattta tgccaataac acatttccgg cagaattcat ctatcagaat
    10381 ctcatgattg agagtaatat tattcatgca gctcacaaag ccggagttaa aaaattactt
    10441 tttttaggct caagctgtat ttatcctaaa tttgcagagc agccgatgaa tgagtctgcg
    10501 cttttaactg gcatacttga gccaactaat gagccatacg caattgctaa aattgcgggc
    10561 ataaaattat gtgaatctta taaccgtcaa tatggccgtg attatcgcag tgtaatgcct
    10621 actaatcttt atggcataaa tgataatttt caccatgaaa actctcatgt tattccggca
    10681 ctcatgcgta gattccatga agcaaaagag agtggtgcac cagaggttat tgtctgggga
    10741 accggaacac cgatgcgtga gtttttatat gttgatgata tggctgccgc atctgttcat
    10801 gtaatggaac tcgacgaaga aatttatcaa aaaaatacac agcctatgtt atctcatatt
    10861 aatgttggta cgggtgtgga ttgttctata cgtgaaatgg ctgaaacaat ggcctctgtg
    10921 gtgggttatc aaggtaaaat tgtttttgat gctaccaaac ctgatggcac tccgcgtaaa
    10981 cttatggatg taacccggct caaaaacttg ggttggcaat atcgctataa tttacatgaa
    11041 ggcttatcat taacatataa atggtttatt gagaatatta attcttttcg gggatagtta
    11101 tgaacaagag attgggatgt gacttattca aaacaatagt tgagcatact cctctaatct
    11161 cgattgatct cataattaga aacgataaag gagaggcgct gctggggcag cgcctgaatc
    11221 gcccagcaca aaattgttgg tttgtgccag gtgggcgaat ttataaggat gagtcattcg
    11281 aggttgcatt taaacggata acatttgaag agttgggcgt tcaaattagt cttaatgatg
    11341 ccttatttct tggagtgtat gaacattttt acgatgataa tttttctgaa gcggaatttt
    11401 ctacacacta cgtagtgctt ggatatgaaa tccaaattaa accgcagcaa cttcacctac
    11461 caacggtcca acacaattct tacaaatggt ttgatgtagt aacgttgctg aatagcacta
    11521 tagttcatca atataccaaa aattatttta taccaaggta atagatatgc tacttcctgt
    11581 tgttatggcc ggtggttccg gtaccagatt atggcctctt tcacgtacac tttatccgaa
    11641 acaatttctg tctttaaata gtcgtttaac catgttgcaa gagacattgc ggcggcttga
    11701 caaggtcgaa cataaacccg ctttggtcat ttgtaacgaa tcacatcgct ttatcgttgc
    11761 tgaacaattg cgtaaagaag gtttaaagca tagtggtatt ttgctcgagc ctgttggtcg
    11821 taatactgcg cctgctgtag cactctcagc acttcaggct atggtaactg gagatgatcc
    11881 tcttctgttg gttcttgctg ctgatcatga aatccaggat gaggataatt tcattgctgc
    11941 agttcttgct gccaagaatt tagcagagca gggtaagctt gttacgtttg gtattgttcc
    12001 aacatcccct gagactggct atggttacat taagtcaggt gaatctctgg atggacaagg
    12061 ttacaaagtt gcagcttttg ttgaaaaacc agatcttcac gtagctcagc ggtacatatc
    12121 aaatggcggt tatctttgga atagtgggat gtttatgttc agagcatctg tatttatcga
    12181 tgaactgaaa aaattccgac cagatatttt agccagttgc caaagctccc tgtcctcttc
    12241 gatacaagat ttagatttta tccgcctgga taacgcttca ttttcttgct gtcctgaaga
    12301 gtctattgac tatgccgtta tggaaaaaac agccgaagct gtcgtcgttc cattaaatgc
    12361 gcaatggagt gatgtcgggt catggtctgc attgtgggaa ataagttcaa aagaccaaag
    12421 cggcaatgcc attcgtggtg atgtattagt tgaagatgct acaaatagtt atctttattc
    12481 acagcataga cttattggtg ctgtgggcgt aaaggatttg gttgttgttg aaacgaaaga
    12541 tgcagtgtta gttgctcata aagataaagt tcagcaagtt aaaaacatcg tcgctcaact
    12601 taaaaagaat aatcgaacag aatatttaca gcatcgggaa atttttcgac cttggggcag
    12661 tcatgatact atagctgaag ggccgcgctt tcaggtgaaa catgtgattg tattacctgg
    12721 tcacattacc gctaaacaga ttcattacca tcgtactgag cattggattg ttgtctcggg
    12781 aacagctaaa gttcatcttg aagataagac ttaccttgtt tctgaaaatg aatcaacata
    12841 tatacctgtt ggtgttccac atgccattga aaacccggga aaaatacctt tagaaattat
    12901 tgaggttaga tcaggagtct acctggaaga ggatgatgtt ataagagttt cttcttctgg
    12961 agtaggatac taatgcgaat ttctattatc acagctactt ataatagtga aaaaactctc
    13021 cttgatacat tggatgcgca tatgaaagtc agtattatta ccgtgactta taacagcgag
    13081 aaaacaatta aagacacttt agattcggtt gctgtgcaga catataataa tattgagtat
    13141 attattgttg atggtgcttc ttctgatgca acgttgcaca ttattaataa ctcttcagct
    13201 tcagtcgcca aatgtatttc ggaaagagat aagggtatct atgatgcact taataaaggt
    13261 gtacatttag caacaggtga tataattgga tttattcact ctgacgatat attggcgagg
    13321 ccagacatta tagaaacaat tgttattgag tttcagaaaa caaaagctga tattgtttat
    13381 ggtgatctgg ttttcgttga taggagcgat gtcaataaca ttgtaagata ctggcatagt
    13441 ggttctttta gaaaaagtaa aatatcttta ggatgggcgc ctccacatcc ttctttttat
    13501 atgaagagaa gtttatataa ggaagacggt ttttttgatt tgacctttaa aatcgctgct
    13561 gattacgatc agatgattcg agttctgaaa agagagcgta ttaaggttat ttaccttcct
    13621 caagtcttcg ttaaaatgag attgggggga gagagcacga aacttgataa tacagtagct
    13681 agtacaaaag aaattatttc tataatgaaa aaacatcata taaattggta tattgcgatc
    13741 gttaccagaa aattttctaa attgactcaa ctcttcagaa aatcataatc atatctatat
    13801 gcatggaatg tatatttaat gaaaaaatta acctgcttta aagcctatga tattcgcggg
    13861 aaattaggcg aagaactgaa tgaagatatt gcctggcgta ttgggcgtgc ctatggcgaa
    13921 tttctcaaac cgaaaaccat tgtgttaggc ggtgatgtcc gcctcaccag cgaaacctta
    13981 aaactggcgc tggcgaaagg tttgcaagat gcgggcgtcg atgtgctgga tattggcatg
    14041 tccggcaccg aagagatcta tttcgccacg ttccatctcg gcgtggatgg cggcatcgaa
    14101 gttaccgcca gccataaccc gatggattac aacggcatga aactcgtgcg cgaaggggct
    14161 cgcccgatca gcggtgatac cggactgcgc gatgtccagc gcctggcaga agccaacgac
    14221 tttcctcctg tcgatgaaac caaacgcggt cgctatcagc aaatcaatct gcgtgacgct
    14281 tacgttgatc acctgttcgg ttatatcaac gtcaaaaacc tcacgccgct caagctggtg
    14341 attaactccg ggaacggcgc ggcgggtccg gtggtggacg ccattgaagc ccgctttaaa
    14401 gccctcggcg caccagtgga attcatcaaa gtgcacaata cgccggacgg caatttcccc
    14461 aacggtattc ctaacccgct gctgccggaa tgccgcgacg gcacccgtaa tgcggtcatt
    14521 aaacacggcg cggatatggg cattgccttt gacggtgatt ttgaccgctg tttcctgttt
    14581 gacgaaaaag gtcagtttat cgagggctac tacattgtcg gcctgttggc agaagcgttc
    14641 ctcgaaaaaa atcccggcgc gaagatcatc cacgatccgc gtctctcctg gaataccgtt
    14701 gatgtggtga ctgctgctgg cggcactccg gtgatgtcga aaaccggaca cgcctttatt
    14761 aaagaacgta tgcgcaagga agacgccatc tacggtggcg aaatgagcgc ccaccattac
    14821 ttccgtgatt tcgcttactg cgacagcggc atgatcccgt ggctgctggt agccgaactg
    14881 gtgtgtctga aaggaaaaac gctgggcgaa ctggtgcgcg accggatggc ggcgtttccg
    14941 gcaagcggtg agatcaacag caaactggcg caccccgttg aggcgattaa ccgcgtggaa
    15001 cagcatttta gccgtgaggc gctggcggtg gatcgcaccg atggcatcag catgaccttt
    15061 gccgactggc gctttaacct gcgcacctcc aacaccgaac cggtggtgcg gttgaatgtg
    15121 gaatctcgcg gtgatgtgcc gctgatggaa gaaaagacaa aacttatcct tgagttactg
    15181 aacaagtaat tcagtaattt catataaata agttttaaat gacggaaaag gtgagatatt
    15241 cagtgtggta tagcaaaggt aatgctattc accatctcta tgagtgagtt aacatctata
    15301 ccacatttaa gccgcacact cggcggtgac caccccctga caggagtaaa caatgtcaaa
    15361 gcaacagatc ggcgtagtcg gtatggcagt gatg
//
